LOCUS       DAPCMU010000001        77937 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPCMU010000001 DAPCMU010000000
VERSION     DAPCMU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 77937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.001"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..356
                     /locus_tag="RD744_000001"
     CDS             <1..356
                     /locus_tag="RD744_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDU0556038.1"
                     /translation="EIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKS
                     LEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGN
                     VFKIEYTKKRQRLAY"
     gene            591..827
                     /locus_tag="RD744_000002"
     CDS             591..827
                     /locus_tag="RD744_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556039.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            834..2786
                     /locus_tag="RD744_000003"
     CDS             834..2786
                     /locus_tag="RD744_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDU0556040.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="RD744_000004"
     CDS             complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="RD744_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDU0556041.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3717..4757
                     /locus_tag="RD744_000005"
     CDS             3717..4757
                     /locus_tag="RD744_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDU0556042.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4855..5043
                     /locus_tag="RD744_000006"
     CDS             4855..5043
                     /locus_tag="RD744_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDU0556043.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5101..5454)
                     /locus_tag="RD744_000007"
     CDS             complement(5101..5454)
                     /locus_tag="RD744_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556044.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5985..6407
                     /locus_tag="RD744_000008"
     CDS             5985..6407
                     /locus_tag="RD744_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0556045.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6404..6634
                     /locus_tag="RD744_000009"
     CDS             6404..6634
                     /locus_tag="RD744_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556046.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7132..7332
                     /locus_tag="RD744_000010"
     CDS             7132..7332
                     /locus_tag="RD744_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0556047.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7359..8552
                     /locus_tag="RD744_000011"
     CDS             7359..8552
                     /locus_tag="RD744_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0556048.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8620..10185)
                     /gene="guaA"
                     /locus_tag="RD744_000012"
     CDS             complement(8620..10185)
                     /gene="guaA"
                     /locus_tag="RD744_000012"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDU0556049.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10244..11167)
                     /locus_tag="RD744_000013"
     CDS             complement(10244..11167)
                     /locus_tag="RD744_000013"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDU0556050.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11363..11968
                     /locus_tag="RD744_000014"
     CDS             11363..11968
                     /locus_tag="RD744_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0556051.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12276..13292
                     /locus_tag="RD744_000015"
     CDS             12276..13292
                     /locus_tag="RD744_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0556052.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13433..14734
                     /locus_tag="RD744_000016"
     CDS             13433..14734
                     /locus_tag="RD744_000016"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDU0556053.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14780..15442
                     /gene="deoC"
                     /locus_tag="RD744_000017"
     CDS             14780..15442
                     /gene="deoC"
                     /locus_tag="RD744_000017"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDU0556054.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15463..15861
                     /locus_tag="RD744_000018"
     CDS             15463..15861
                     /locus_tag="RD744_000018"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDU0556055.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15927..17009
                     /locus_tag="RD744_000019"
     CDS             15927..17009
                     /locus_tag="RD744_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0556056.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17221..18783
                     /locus_tag="RD744_000020"
     CDS             17221..18783
                     /locus_tag="RD744_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556057.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18776..19909
                     /locus_tag="RD744_000021"
     CDS             18776..19909
                     /locus_tag="RD744_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556058.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19909..20865
                     /locus_tag="RD744_000022"
     CDS             19909..20865
                     /locus_tag="RD744_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556059.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20964..22130
                     /gene="deoB"
                     /locus_tag="RD744_000023"
     CDS             20964..22130
                     /gene="deoB"
                     /locus_tag="RD744_000023"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDU0556060.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22154..22972
                     /locus_tag="RD744_000024"
     CDS             22154..22972
                     /locus_tag="RD744_000024"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDU0556061.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22989..23696
                     /gene="deoD"
                     /locus_tag="RD744_000025"
     CDS             22989..23696
                     /gene="deoD"
                     /locus_tag="RD744_000025"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDU0556062.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23994..24680)
                     /gene="gpmA"
                     /locus_tag="RD744_000026"
     CDS             complement(23994..24680)
                     /gene="gpmA"
                     /locus_tag="RD744_000026"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDU0556063.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24852..25433)
                     /locus_tag="RD744_000027"
     CDS             complement(24852..25433)
                     /locus_tag="RD744_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0556064.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25566..26249)
                     /gene="rpiA"
                     /locus_tag="RD744_000028"
     CDS             complement(25566..26249)
                     /gene="rpiA"
                     /locus_tag="RD744_000028"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDU0556065.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26568..26981
                     /gene="rpsL"
                     /locus_tag="RD744_000029"
     CDS             26568..26981
                     /gene="rpsL"
                     /locus_tag="RD744_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDU0556066.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27065..27535
                     /gene="rpsG"
                     /locus_tag="RD744_000030"
     CDS             27065..27535
                     /gene="rpsG"
                     /locus_tag="RD744_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDU0556067.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27629..29716
                     /gene="fusA"
                     /locus_tag="RD744_000031"
     CDS             27629..29716
                     /gene="fusA"
                     /locus_tag="RD744_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDU0556068.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29888..31075
                     /gene="tuf"
                     /locus_tag="RD744_000032"
     CDS             29888..31075
                     /gene="tuf"
                     /locus_tag="RD744_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDU0556069.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31482..31790
                     /gene="rpsJ"
                     /locus_tag="RD744_000033"
     CDS             31482..31790
                     /gene="rpsJ"
                     /locus_tag="RD744_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDU0556070.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31821..32450
                     /gene="rplC"
                     /locus_tag="RD744_000034"
     CDS             31821..32450
                     /gene="rplC"
                     /locus_tag="RD744_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDU0556071.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32478..33101
                     /gene="rplD"
                     /locus_tag="RD744_000035"
     CDS             32478..33101
                     /gene="rplD"
                     /locus_tag="RD744_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDU0556072.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            33101..33391
                     /gene="rplW"
                     /locus_tag="RD744_000036"
     CDS             33101..33391
                     /gene="rplW"
                     /locus_tag="RD744_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDU0556073.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33430..34263
                     /gene="rplB"
                     /locus_tag="RD744_000037"
     CDS             33430..34263
                     /gene="rplB"
                     /locus_tag="RD744_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDU0556074.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34306..34584
                     /gene="rpsS"
                     /locus_tag="RD744_000038"
     CDS             34306..34584
                     /gene="rpsS"
                     /locus_tag="RD744_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDU0556075.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34607..34954
                     /gene="rplV"
                     /locus_tag="RD744_000039"
     CDS             34607..34954
                     /gene="rplV"
                     /locus_tag="RD744_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDU0556076.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34968..35624
                     /gene="rpsC"
                     /locus_tag="RD744_000040"
     CDS             34968..35624
                     /gene="rpsC"
                     /locus_tag="RD744_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDU0556077.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35627..36061
                     /gene="rplP"
                     /locus_tag="RD744_000041"
     CDS             35627..36061
                     /gene="rplP"
                     /locus_tag="RD744_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDU0556078.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            36051..36239
                     /gene="rpmC"
                     /locus_tag="RD744_000042"
     CDS             36051..36239
                     /gene="rpmC"
                     /locus_tag="RD744_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDU0556079.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36264..36530
                     /gene="rpsQ"
                     /locus_tag="RD744_000043"
     CDS             36264..36530
                     /gene="rpsQ"
                     /locus_tag="RD744_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDU0556080.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36588..36956
                     /gene="rplN"
                     /locus_tag="RD744_000044"
     CDS             36588..36956
                     /gene="rplN"
                     /locus_tag="RD744_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDU0556081.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36993..37301
                     /gene="rplX"
                     /locus_tag="RD744_000045"
     CDS             36993..37301
                     /gene="rplX"
                     /locus_tag="RD744_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDU0556082.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37329..37868
                     /gene="rplE"
                     /locus_tag="RD744_000046"
     CDS             37329..37868
                     /gene="rplE"
                     /locus_tag="RD744_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDU0556083.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37887..38072
                     /locus_tag="RD744_000047"
     CDS             37887..38072
                     /locus_tag="RD744_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDU0556084.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            38108..38506
                     /gene="rpsH"
                     /locus_tag="RD744_000048"
     CDS             38108..38506
                     /gene="rpsH"
                     /locus_tag="RD744_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDU0556085.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38538..39074
                     /gene="rplF"
                     /locus_tag="RD744_000049"
     CDS             38538..39074
                     /gene="rplF"
                     /locus_tag="RD744_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDU0556086.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39235..39591
                     /gene="rplR"
                     /locus_tag="RD744_000050"
     CDS             39235..39591
                     /gene="rplR"
                     /locus_tag="RD744_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDU0556087.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39612..40112
                     /gene="rpsE"
                     /locus_tag="RD744_000051"
     CDS             39612..40112
                     /gene="rpsE"
                     /locus_tag="RD744_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDU0556088.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40127..40306
                     /gene="rpmD"
                     /locus_tag="RD744_000052"
     CDS             40127..40306
                     /gene="rpmD"
                     /locus_tag="RD744_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDU0556089.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40350..40790
                     /gene="rplO"
                     /locus_tag="RD744_000053"
     CDS             40350..40790
                     /gene="rplO"
                     /locus_tag="RD744_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDU0556090.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40790..42085
                     /gene="secY"
                     /locus_tag="RD744_000054"
     CDS             40790..42085
                     /gene="secY"
                     /locus_tag="RD744_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDU0556091.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42147..42794
                     /locus_tag="RD744_000055"
     CDS             42147..42794
                     /locus_tag="RD744_000055"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDU0556092.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42985..43203
                     /gene="infA"
                     /locus_tag="RD744_000056"
     CDS             42985..43203
                     /gene="infA"
                     /locus_tag="RD744_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDU0556093.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43236..43352
                     /gene="rpmJ"
                     /locus_tag="RD744_000057"
     CDS             43236..43352
                     /gene="rpmJ"
                     /locus_tag="RD744_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDU0556094.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43370..43735
                     /gene="rpsM"
                     /locus_tag="RD744_000058"
     CDS             43370..43735
                     /gene="rpsM"
                     /locus_tag="RD744_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDU0556095.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43763..44152
                     /gene="rpsK"
                     /locus_tag="RD744_000059"
     CDS             43763..44152
                     /gene="rpsK"
                     /locus_tag="RD744_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDU0556096.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44232..45170
                     /locus_tag="RD744_000060"
     CDS             44232..45170
                     /locus_tag="RD744_000060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDU0556097.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45198..45578
                     /gene="rplQ"
                     /locus_tag="RD744_000061"
     CDS             45198..45578
                     /gene="rplQ"
                     /locus_tag="RD744_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDU0556098.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45850..46689
                     /locus_tag="RD744_000062"
     CDS             45850..46689
                     /locus_tag="RD744_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0556099.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46665..47534
                     /locus_tag="RD744_000063"
     CDS             46665..47534
                     /locus_tag="RD744_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0556100.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47524..48321
                     /locus_tag="RD744_000064"
     CDS             47524..48321
                     /locus_tag="RD744_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDU0556101.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48524..49270
                     /gene="truA"
                     /locus_tag="RD744_000065"
     CDS             48524..49270
                     /gene="truA"
                     /locus_tag="RD744_000065"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDU0556102.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49331..49537)
                     /locus_tag="RD744_000066"
     CDS             complement(49331..49537)
                     /locus_tag="RD744_000066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556103.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(49603..52272)
                     /locus_tag="RD744_000067"
     CDS             complement(49603..52272)
                     /locus_tag="RD744_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDU0556104.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52510..52698)
                     /locus_tag="RD744_000068"
     CDS             complement(52510..52698)
                     /locus_tag="RD744_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDU0556105.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52968..53330
                     /locus_tag="RD744_000069"
     CDS             52968..53330
                     /locus_tag="RD744_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0556106.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53382..55064
                     /locus_tag="RD744_000070"
     CDS             53382..55064
                     /locus_tag="RD744_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDU0556107.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            55181..57217
                     /gene="recG"
                     /locus_tag="RD744_000071"
     CDS             55181..57217
                     /gene="recG"
                     /locus_tag="RD744_000071"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDU0556108.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57266..58267
                     /gene="plsX"
                     /locus_tag="RD744_000072"
     CDS             57266..58267
                     /gene="plsX"
                     /locus_tag="RD744_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDU0556109.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58389..58631
                     /gene="acpP"
                     /locus_tag="RD744_000073"
     CDS             58389..58631
                     /gene="acpP"
                     /locus_tag="RD744_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDU0556110.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58899..59903
                     /locus_tag="RD744_000074"
     CDS             58899..59903
                     /locus_tag="RD744_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556111.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59904..60848
                     /locus_tag="RD744_000075"
     CDS             59904..60848
                     /locus_tag="RD744_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556112.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60848..61810
                     /locus_tag="RD744_000076"
     CDS             60848..61810
                     /locus_tag="RD744_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556113.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61837..62751
                     /locus_tag="RD744_000077"
     CDS             61837..62751
                     /locus_tag="RD744_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556114.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62777..64558
                     /locus_tag="RD744_000078"
     CDS             62777..64558
                     /locus_tag="RD744_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0556115.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64906..65592
                     /locus_tag="RD744_000079"
     CDS             64906..65592
                     /locus_tag="RD744_000079"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDU0556116.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65612..69193
                     /gene="smc"
                     /locus_tag="RD744_000080"
     CDS             65612..69193
                     /gene="smc"
                     /locus_tag="RD744_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDU0556117.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69202..70011
                     /locus_tag="RD744_000081"
     CDS             69202..70011
                     /locus_tag="RD744_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0556118.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            70022..71020
                     /gene="ftsY"
                     /locus_tag="RD744_000082"
     CDS             70022..71020
                     /gene="ftsY"
                     /locus_tag="RD744_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDU0556119.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71207..73474
                     /gene="gshAB"
                     /locus_tag="RD744_000083"
     CDS             71207..73474
                     /gene="gshAB"
                     /locus_tag="RD744_000083"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDU0556120.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73563..74015)
                     /locus_tag="RD744_000084"
     CDS             complement(73563..74015)
                     /locus_tag="RD744_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDU0556121.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            74165..75115
                     /locus_tag="RD744_000085"
     CDS             74165..75115
                     /locus_tag="RD744_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDU0556122.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            75108..76067
                     /locus_tag="RD744_000086"
     CDS             75108..76067
                     /locus_tag="RD744_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDU0556123.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            76064..76819
                     /locus_tag="RD744_000087"
     CDS             76064..76819
                     /locus_tag="RD744_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0556124.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76842..77795
                     /locus_tag="RD744_000088"
     CDS             76842..77795
                     /locus_tag="RD744_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0556125.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        25516 a        14022 c        16638 g        21761 t
ORIGIN      
        1 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
       61 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
      181 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
      241 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      301 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tattaagtag
      361 ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa tgagtatcaa attgcaactg
      421 aatttgatac tcgtttttta ttgttaaatc aacgtttgtt gttaatttac accacaaaat
      481 gtaagttaaa atgtatataa aaatgcgaac gtgaaataat tattgtagga atgcaaattg
      541 gtgtgttaaa atggttacaa tttactaagc aaaaaggggt gcattttcct atgcaatttc
      601 ttgagccaaa aaataagaac gctaagtctg tggattggga aatatcagag caagtaaggg
      661 tgatagttaa gcaatacgct gaatatgcag agcgtactga aagcgaagca gtggatgaat
      721 tcttattgaa cattttagac gataagaagt ttattgaatg gattgccaac aagcgaagta
      781 ataagcggat tgttgaaaaa atgggtatca aagatagagt gggttgatag acaatgacca
      841 agcttaaaag attagccacg aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa
      901 acgaagaatt aaaagatagg gctaaacaat atgatttatt aactccaaaa cgatttgcaa
      961 caaggtataa caaaatgttg ttcttgccaa cgtcttttaa atggaacggt tctgaatatc
     1021 ccattcaata taattactgc atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca
     1081 aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat gactggtagt tccaaagata
     1141 aaggtcataa aggtatgtac tgcaatgaca atcctattgg cagaggtgta tcgcaaaact
     1201 gtacagtaac ccctttatct aattggtctg ttgttgaaga aattaaacgc cttatagaaa
     1261 tcaatagcat ccaagatgtt gagccagatt atcaatttca taaagaaggc tgctcggaag
     1321 aagaatcaac cccattcaat gagccgaagc aattttacaa aagggggaaa agtaggggca
     1381 aatctcaacg ctatcaatgt aaggcgtgta agaagttcac taatgttcta ccaaaaagag
     1441 aagaaaccac tacttatcac caacaaaaga acaccattct tccaatgttc gctaagatgg
     1501 ttgttggaag ggtttcagtc agtcgtactt gtgatatttt aggaattgga gttggaacct
     1561 actatcataa gctggagtgg ctgtataggc gttgtttgga gtttttagaa cgatatgaga
     1621 cacaacccct tcaaacaaag aaatttaacg agatgtggct taatacggac aagatgcact
     1681 actacctaaa taacgtccgt aagaaaggac aaggttcaaa gaaatatacg gggtttgaag
     1741 accttaatat gcaaacgtat atagttgtat ctgctgaggt cttatcaaga tatgtgttcc
     1801 gttctgatgt tgcctatgat tggaatattt caatggatga attaaatgaa gatacaagaa
     1861 aattcaaaga agaccatctc aatactttta gtcgtaaaaa tgataggtta gattggtctt
     1921 attatccaca agaaccatcc gctaacgata gcgaaaatag gaatgcttat cttcacgaat
     1981 tagggaaaat tactaatagg agtagatttg tcgatggttt aaatgttgat gctccttata
     2041 ctacaactgc tcactattgg ttaattaaac aaatggttaa tgccgacgaa tggcgaatga
     2101 ttagtgatga tgacttctct attagaaacg ccttttatag agtattcacg aaagaattaa
     2161 ggttgagcga tgcacatcat tttatttgcc aagttaacaa gacgaaaagc agaaaacaat
     2221 gcttaaaaga attcggacaa gcaaaagcag aactgctcga ttggggagat atcagaggat
     2281 tcaaaacaaa gtttctacgg actttggcat ctcattatct aactgaatta ctaacaagtc
     2341 atcaattcca cgaagaggct attagtaaag atggagaaag atatcgcaaa tatgccgata
     2401 accctatcaa acatccatta gccactaagg ataaaggatt ttattcagtt gattgtagaa
     2461 cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc
     2521 attcgaccaa tagctttatt caacagataa gacgttatat atcatctctt gaaagaccat
     2581 taacaacagc tcgtggtgat aagaagagct atatttacgc taacttcaat ccgaagtatg
     2641 ctcaatttgc gattacaatt ctaagaactt actacaactt ttgtcgccct tttaaatcag
     2701 cggataaaaa ggttttgaca cctgctcaaa gattagggat tacagataaa caatttgatt
     2761 ggaaagatat aatttatttc aaatgacaaa aagagcttgg agtaatccca agctcttgtg
     2821 ctttatcttt actgttcttc ttcaattaac gcacccgtta actcaataag ttttttcttt
     2881 catatacatg aaaatagtag ttatacggat ttttatcatt ctttatccct ttttgggcaa
     2941 atacctcatt ccattcctca taattcactt ctggaaaaaa agtatctcct tcgaattcat
     3001 gatgtatttt tgtgatgtac attttctcaa cataagggaa aaacaaatta taaatctgtt
     3061 ctcctccgaa aataaaaatt tcttcttcgt ttttacataa ctcaaaaaca tcttctattg
     3121 aatgaacaat ttcacaacca ttaaaggtaa accccttatc tctcgtcaga ataatatttc
     3181 ttctgtcagg taaggctctt ccgattgatt caaggttctt cctacctaat attatcggat
     3241 gtccctttgt agtattttta acatattccc agtccttggg aatcctccaa ggaatgtcat
     3301 tctctttgcc aatcactcta ttcttatcca tcgcagcaat caaagaaact ttcatttata
     3361 acacagcctt tttaactaat ttaccatcta atttcttatt tctatcaaaa aaccttttct
     3421 cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt atcataatac
     3481 cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata aatcattttg
     3541 tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag tacattttgt
     3601 cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat agtggagtac
     3661 tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt
     3721 atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta
     3781 ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac
     3841 agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac
     3901 ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc
     3961 tacgatatta cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac
     4021 aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag
     4081 agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg
     4141 aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg
     4201 gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa
     4261 atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt tggaatgtga
     4321 cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt actccagcag
     4381 attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct ggaacagaat
     4441 tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt tcacgggcat
     4501 ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg
     4561 tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat gatttaactt
     4621 tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag gttaccactg
     4681 gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta agatatatgt
     4741 tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct tattaaataa
     4801 gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat tgttattata
     4861 aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg aatacgagtg
     4921 gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga aactttaatt
     4981 aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac gcagagccga
     5041 taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga attttactga
     5101 ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca ttaaggatag
     5161 ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag tgcttttaat
     5221 caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg atacaagatc
     5281 atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta aataacgtgg
     5341 gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca ttcgagcctc
     5401 tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac gcatactaaa
     5461 tcacctctat acattttact gttcctatat aaccataaac aggtatagaa aaacatgtta
     5521 tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga atccaatttc
     5581 aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta gtcatactcc
     5641 tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag aaagataatc
     5701 tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct gtactggata
     5761 gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa gcatatgaac
     5821 agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt ttaggatttc
     5881 tcttcattta tgcaacaaat cccccgttcg tgtcggggtt tagtatgaag ggagataaag
     5941 aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa ccatcttctt
     6001 ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt gaagatgaac
     6061 gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg ttttctgata
     6121 tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact gatttccaac
     6181 tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt gaagccctgc
     6241 gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg gatatgagtg
     6301 atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt catcgaataa
     6361 gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa cagcttatcc
     6421 aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag caattaatca
     6481 gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca tgacagacga
     6541 atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg aaacgaaact
     6601 catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg aagcgtgtcg
     6661 aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca tattgtgtgt
     6721 tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat aaaggcaatc
     6781 gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta caaaattagt
     6841 gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc atgactttct
     6901 tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg tcacaggcaa
     6961 tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt gctagagctg
     7021 ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat ttgatataat
     7081 tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat aatgataacg
     7141 gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc agacatcata
     7201 aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc tcgaaagcta
     7261 gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc aaatgaaaac
     7321 aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac ccaataaaaa
     7381 aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct aaagggaaaa
     7441 aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa gaatattcga
     7501 aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg acgtttaaac
     7561 attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc agaacatatg
     7621 aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg gctgtttccg
     7681 atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag tgtaagtctt
     7741 cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca attgtccttg
     7801 ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa aagtctaaaa
     7861 ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac aaagaagatt
     7921 actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga atgcgaattg
     7981 gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta ctaaccatca
     8041 acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca aaaacaaagg
     8101 cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc gaatggaagg
     8161 aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt attcctaccc
     8221 aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt atccatcgaa
     8281 tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg ggagaaaatc
     8341 cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta ggaacgtatg
     8401 gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga gccatttcct
     8461 ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact gtagactact
     8521 tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata agaagagcca
     8581 gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact ccactgttgc
     8641 tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat
     8701 acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc
     8761 gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat
     8821 aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag
     8881 tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt
     8941 gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg
     9001 ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa
     9061 taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt
     9121 atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa
     9181 cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc
     9241 aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc
     9301 tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca agccgaattt
     9361 tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac
     9421 aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga
     9481 atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt
     9541 ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc
     9601 aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa
     9661 ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct
     9721 tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca
     9781 tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa
     9841 caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt
     9901 gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg
     9961 atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc
    10021 ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa
    10081 gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa
    10141 tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat
    10201 ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga
    10261 aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg
    10321 taggcaagat atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca
    10381 ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt
    10441 ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg
    10501 gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg
    10561 caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt
    10621 cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt
    10681 cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag
    10741 gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc
    10801 cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca
    10861 gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag
    10921 gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt
    10981 ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca
    11041 tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt
    11101 gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat
    11161 cattcatatt agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg
    11221 caaaccccac cacttaagag agattgccga tctttttgta taatctttat aatagaaaga
    11281 aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa
    11341 agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc
    11401 gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga
    11461 ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt
    11521 cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc
    11581 gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga
    11641 acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat
    11701 ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa
    11761 tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg
    11821 gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc
    11881 gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata
    11941 ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt
    12001 ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa
    12061 aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg
    12121 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
    12181 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
    12241 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
    12301 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
    12361 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
    12421 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
    12481 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
    12541 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
    12601 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
    12661 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
    12721 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
    12781 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
    12841 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
    12901 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
    12961 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
    13021 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
    13081 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
    13141 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
    13201 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
    13261 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
    13321 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
    13381 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
    13441 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
    13501 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
    13561 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
    13621 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
    13681 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
    13741 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
    13801 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
    13861 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
    13921 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
    13981 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
    14041 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
    14101 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
    14161 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
    14221 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
    14281 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
    14341 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
    14401 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
    14461 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
    14521 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
    14581 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
    14641 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
    14701 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
    14761 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
    14821 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
    14881 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
    14941 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
    15001 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
    15061 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
    15121 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
    15181 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
    15241 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
    15301 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
    15361 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
    15421 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
    15481 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
    15541 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
    15601 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
    15661 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
    15721 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
    15781 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
    15841 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
    15901 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
    15961 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
    16021 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
    16081 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
    16141 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
    16201 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
    16261 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
    16321 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
    16381 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
    16441 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
    16501 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
    16561 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
    16621 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
    16681 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
    16741 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
    16801 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
    16861 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
    16921 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
    16981 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
    17041 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
    17101 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
    17161 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
    17221 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
    17281 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
    17341 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
    17401 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
    17461 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
    17521 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
    17581 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
    17641 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
    17701 ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat
    17761 gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg
    17821 cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa
    17881 ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt
    17941 ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg
    18001 tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac
    18061 ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg
    18121 gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa
    18181 ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg
    18241 ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca
    18301 ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg
    18361 aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt
    18421 cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac
    18481 cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc
    18541 gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc
    18601 agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga
    18661 gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg
    18721 gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa
    18781 tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt
    18841 gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc
    18901 tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac
    18961 gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat
    19021 tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt
    19081 tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg
    19141 tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat
    19201 cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt
    19261 gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc
    19321 actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt
    19381 tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt
    19441 tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa
    19501 acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt
    19561 tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt
    19621 tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc
    19681 gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc
    19741 gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct
    19801 gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac
    19861 aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc
    19921 gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg
    19981 tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc
    20041 atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt
    20101 tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg
    20161 attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt
    20221 aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa
    20281 actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg
    20341 gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt
    20401 gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt
    20461 ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca
    20521 ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt
    20581 tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg
    20641 atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct
    20701 caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg
    20761 ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct
    20821 tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa
    20881 aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa
    20941 ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag
    21001 taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct gatacattag
    21061 gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa
    21121 cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat
    21181 tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga
    21241 atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa
    21301 tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta
    21361 ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg
    21421 atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga
    21481 tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg
    21541 cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg
    21601 cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga
    21661 ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca
    21721 cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag
    21781 gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag
    21841 tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga
    21901 tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg
    21961 gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac
    22021 aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta
    22081 gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa
    22141 taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct
    22201 tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt
    22261 agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt
    22321 ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt
    22381 gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta
    22441 tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg
    22501 tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt
    22561 tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga
    22621 tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact
    22681 aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc
    22741 tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc
    22801 agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa
    22861 cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag
    22921 agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt aataggagga
    22981 acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac
    23041 ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt
    23101 gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt
    23161 ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc
    23221 agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg
    23281 tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa
    23341 aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat
    23401 atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt
    23461 ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag
    23521 tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa
    23581 gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc
    23641 aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag
    23701 gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca
    23761 tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa
    23821 gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt
    23881 tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa accgtttata
    23941 cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt tttttatagg
    24001 tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg ttgacctgtt
    24061 ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt agctagggcg
    24121 cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa tgctggagca
    24181 atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt ttctccacca
    24241 ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga accttcgtca
    24301 gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg cacttgttcg
    24361 tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc atagtgacgt
    24421 tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata ttctaagatc
    24481 aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc aaattcgatt
    24541 ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc tggtgctaga
    24601 tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag accgtgacga
    24661 gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata tttctgaaat
    24721 tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa aattagataa
    24781 gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac gcaactgttc
    24841 gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt gatatattta
    24901 taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt ttcttttttt
    24961 tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg tcttccgccg
    25021 atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt gatcaatgcg
    25081 cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat ttcgcgcgta
    25141 tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa ttgcgtgagt
    25201 tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac tagtgcatcg
    25261 ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc attttgttcc
    25321 acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg atcaacctga
    25381 gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt catggtgatt
    25441 cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat aaagtacgtc
    25501 aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga tcaagcagtt
    25561 tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga gccgacaact
    25621 actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag ttcatctaaa
    25681 taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat gatgtaatga
    25741 ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg ttggaatctt
    25801 ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc aactgggagt
    25861 gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat cgtttctttt
    25921 gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt gatcccttga
    25981 aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg gacttcatca
    26041 atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt tgaagtagta
    26101 actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc taccatataa
    26161 taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa tttcgcggct
    26221 tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta gtttgagcat
    26281 cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc ttttttattc
    26341 caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc attcgtgata
    26401 ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac tgtttatcaa
    26461 agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa aagaaccacc
    26521 tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg cctacaatta
    26581 accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca cctgctttga
    26641 acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct caaaaacgtg
    26701 gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct ttacgtaaat
    26761 atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca gggattggtc
    26821 ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa gacttaccag
    26881 gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac gaccgtaaac
    26941 aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta ttaaacaaat
    27001 gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga aggaggagtt
    27061 acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc ctatttataa
    27121 ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac gcgggattgc
    27181 agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta acgatccatt
    27241 agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta aagcacgtcg
    27301 tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc gcgttactct
    27361 aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa tggaagaacg
    27421 cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta aaaaacgtga
    27481 agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct ggtaagattc
    27541 tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt ttagcaacta
    27601 gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac aaaacactcg
    27661 taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg aacgtatctt
    27721 gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt cacaaatgga
    27781 ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta ctacagcaca
    27841 atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact tcacagttga
    27901 agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg cacaatcagg
    27961 tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg tacctcgtat
    28021 cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag tatcaacttt
    28081 acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg cggaagacga
    28141 cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa acgatcttgg
    28201 aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg aagaatggcg
    28261 cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga aatacttaga
    28321 aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa cagttaacgt
    28381 agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc aattactatt
    28441 ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca aagggattga
    28501 ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt tctcagcttt
    28561 agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc gtgtgtactc
    28621 tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac gtgaacgtgt
    28681 tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag tttacgctgg
    28741 agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac tatgtgatga
    28801 aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc aagtcgctat
    28861 tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa aactatctga
    28921 agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa tcatcgctgg
    28981 tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat tcaaagttga
    29041 agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg gcactcaagc
    29101 cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg tatggattga
    29161 atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg tcggtggggt
    29221 tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta tggaaaatgg
    29281 tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg gttcatacca
    29341 tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat tacgtgcagc
    29401 agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag ttgttatccc
    29461 tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgttgaagg
    29521 tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac ttgctgaaat
    29581 gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat ttacaatgac
    29641 atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta agaaaaacgg
    29701 cggaaaagct gaataatttc ttaaaaccgg atagtcctgc aaagagatta tccaaagaga
    29761 ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca tgtggtgtac
    29821 cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt taggaggaca
    29881 ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca ttggtactat
    29941 cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg tactatctaa
    30001 gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag aagaacgcga
    30061 acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac gccactacgc
    30121 tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg gtgctgctca
    30181 aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc aaactcgtga
    30241 acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct tgaacaaagt
    30301 agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc gtgacctatt
    30361 aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag ctttgaaagc
    30421 tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg cagttgacga
    30481 atacatccca actccagaac gtgacaacga caaaccattc atgatgccag ttgaagacgt
    30541 gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg gacaagttcg
    30601 cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa caacagttac
    30661 tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca acattggtgc
    30721 tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag ctaaaccagg
    30781 tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa aagaagaagg
    30841 tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc gtacaactga
    30901 cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg gcgacaacgt
    30961 aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta aattctcaat
    31021 ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag cttaattcac
    31081 ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg aaggtcttag
    31141 gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca ggagtattat
    31201 aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt tatgaaaaca
    31261 aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat aatgacaaag
    31321 gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg gctaagaaac
    31381 gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa attttcgtgg
    31441 agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa caaaaaattc
    31501 gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcggcggat aaaatcgtgg
    31561 aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca actgaacgca
    31621 gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa caattcgaaa
    31681 tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact gttgatgctt
    31741 tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa aactaaaaca
    31801 aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac
    31861 aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct actccaaacg
    31921 tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa gttggttacc
    31981 aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca
    32041 cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa tatgaagtag
    32101 gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta acaggtacta
    32161 cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc ggaccaatgg
    32221 ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg
    32281 tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact atccaaaact
    32341 tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt aatgttcctg
    32401 gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa ttgtaggaag
    32461 aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga agccaaaacg
    32521 gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt gttgtttacg
    32581 atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta
    32641 gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc
    32701 aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga ccaacaccac
    32761 gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt
    32821 cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc gacgcaccaa
    32881 aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa gtattggttg
    32941 ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca aacgtttctg
    33001 tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa
    33061 cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac tagacgtaat
    33121 caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga aatacacttt
    33181 tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag ctgcttttga
    33241 cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa
    33301 atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg aagattcaaa
    33361 agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat taaattagga
    33421 gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg taacatgaca
    33481 ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt acaaccattg
    33541 aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca aggtggcgga
    33601 cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt tgttgcaact
    33661 gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt acactatgaa
    33721 gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat gaaacttgtt
    33781 tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa cattccagtt
    33841 ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt gatccgttct
    33901 gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat ccgcttgaac
    33961 tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc tgtaggtaac
    34021 gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat gcgtaaacgc
    34081 ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg tggtgaaggt
    34141 aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc tcttggttac
    34201 aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg caaaaataaa
    34261 taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg tcgtagttta
    34321 aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca agcaggggtt
    34381 gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc aagttttgtt
    34441 ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat ccaagaagac
    34501 atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg acatgctgct
    34561 gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg cagaacaaat
    34621 cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag cccgtttagt
    34681 aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga aattcacacc
    34741 aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa
    34801 taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta acgaaggacc
    34861 tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca acaaacgtac
    34921 aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag
    34981 tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa tggtatgctg
    35041 aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt atttcaacta
    35101 aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca
    35161 tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa
    35221 gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat atcgtggaaa
    35281 tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt caattagaaa
    35341 accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg cgtgctggcg
    35401 ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc gctcgttcag
    35461 aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt gattacgcat
    35521 gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc tatcgtggag
    35581 aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt tagtacctaa
    35641 acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa
    35701 agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga tcacaaaccg
    35761 ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg
    35821 gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac gtatgggttc
    35881 tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga
    35941 aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt
    36001 accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta
    36061 aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag
    36121 aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact gcacgtatta
    36181 aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat
    36241 tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag
    36301 gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa acaaagaaaa
    36361 accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg catgatgaaa
    36421 acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca ttatcagcta
    36481 caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa taacacgaga
    36541 ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg atccaagcag
    36601 aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg atcaaagtcc
    36661 ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct acggttaaac
    36721 aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa
    36781 caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa aatgctgcag
    36841 tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca gttgcacgtg
    36901 aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt ttataatctt
    36961 gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta
    37021 aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa
    37081 aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag
    37141 ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct aacgtgatgg
    37201 tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac ggcaaaaaag
    37261 tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag gaaggagggt
    37321 cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca tcattgatgg
    37381 aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca
    37441 tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt gaagaattaa
    37501 ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc gctggattcc
    37561 gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa agaatgtacg
    37621 aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc catggtgtaa
    37681 gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa caattgatct
    37741 tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc gttattgtaa
    37801 caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc atgccattcc
    37861 aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac
    37921 gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga cgtccacatt
    37981 cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc tataaaggtc
    38041 aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa
    38101 aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc gtaacgcaaa
    38161 catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg acatcgctga
    38221 aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg acaaacaagg
    38281 cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca caaacttaaa
    38341 acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt
    38401 gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg ataaagaagc
    38461 tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa gtagaatcta
    38521 caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc cctgaaggag
    38581 taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt gaattgactc
    38641 gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc
    38701 caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca
    38761 tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg gttgggtacc
    38821 gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat ccagtagaaa
    38881 tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta attgttaaag
    38941 gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact cgtcctccag
    39001 aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt aaagaaggta
    39061 aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga aaaaggcatt
    39121 tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt ctatttttcg
    39181 aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac aattgtgatt
    39241 acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg taacaatatc
    39301 tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa catctacgct
    39361 caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt ggataaagaa
    39421 atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt tgctgaacgt
    39481 gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct ttaccatggc
    39541 cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta gaagaaggag
    39601 gaataccatt catggtttat atcgatccaa aacatttgga attagaagac cgcgttgttg
    39661 caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt gctgctttag
    39721 ttgttgtcgg tgacaaaaac ggacacgtag ggtttggtac aggtaaagct caagaagttc
    39781 ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa gtacctatgg
    39841 ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga atcctaatga
    39901 aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc gtattagaat
    39961 tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca atcaacgttg
    40021 ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc
    40081 gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg ctgaattaaa
    40141 agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata cagttaaagc
    40201 gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag caattaaagg
    40261 catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta gaaaaatcat
    40321 aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa cctgctgaag
    40381 gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac ggaaaaactg
    40441 ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg
    40501 aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc
    40561 gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac ggagctgaag
    40621 taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag
    40681 tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa ttctctaaag
    40741 cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa tgtttagact
    40801 attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta ctgtatttgt
    40861 gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt
    40921 gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg gtagtgcgat
    40981 gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt ctatcgtcat
    41041 tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt
    41101 tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg catttatcca
    41161 atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg tgaataatcc
    41221 taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga tgtttgttac
    41281 ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt
    41341 tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag attactttat
    41401 aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt tactagtagc
    41461 ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca agatcccgat
    41521 ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa
    41581 tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc caaatgctgt
    41641 tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga tgatggagat
    41701 ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg ttgccttcac
    41761 tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca
    41821 aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg tctcaagatt
    41881 actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat tgcttccaat
    41941 cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta caagcttact
    42001 gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg
    42061 taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca acacacgttc
    42121 tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg ccaggcgcag
    42181 gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac atctcaacag
    42241 gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa gcaaagtctt
    42301 atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac
    42361 tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt acgctagatc
    42421 aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat gctgtcatcg
    42481 atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt atctgcagca
    42541 attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac acatgtgatc
    42601 gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg gttaaaaatc
    42661 gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa gaacaaggtt
    42721 tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa
    42781 tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa
    42841 gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc tagatgatcc
    42901 attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt gaaaatgcga
    42961 aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt cgaaggtaca
    43021 gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt
    43081 cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc tggtgacaaa
    43141 gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta tcgctttaaa
    43201 taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca tcagtaaaac
    43261 caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg atttgcccag
    43321 caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata tggctcgtat
    43381 tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt atatctatgg
    43441 tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg aagatgttcg
    43501 cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag ataaattaaa
    43561 agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg
    43621 ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa
    43681 caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa aataattctt
    43741 aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa
    43801 gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca ctatcatcat
    43861 gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat taggtttcaa
    43921 aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg ctacaaaagc
    43981 agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg gttctggacg
    44041 tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt
    44101 gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc
    44161 gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg
    44221 taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag
    44281 attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact ttagggaatt
    44341 ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat attcaaatcg
    44401 atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc actcaaatca
    44461 tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa
    44521 tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat agtgatgtag
    44581 aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg ttccatgctc
    44641 gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa aaagaagata
    44701 tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc
    44761 aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg atggagattt
    44821 ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa atcatgactg
    44881 aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa atcatggtcg
    44941 aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt
    45001 ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa
    45061 ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca ttagaagaag
    45121 ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa aaccttttac
    45181 gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc caacgtaaag
    45241 cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg actactgaag
    45301 ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt aaacgcggag
    45361 atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg
    45421 aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac gatattgcac
    45481 ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa ccaagacgcg
    45541 gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat aacatcactt
    45601 tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag tctagctcag
    45661 cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag tgttttttta
    45721 taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca ttttaagcga
    45781 gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta ggatgaagaa
    45841 ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata ccagccagat
    45901 gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga gtggatcgct
    45961 attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa tggattgctt
    46021 ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga aaatatttgg
    46081 acgatccgac agatggttgg aatgattttt caaaatccag acaatcagtt tgttggttcg
    46141 acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag agaagaaatg
    46201 ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag
    46261 cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta
    46321 cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga aggtcgtgaa
    46381 gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt tatatctatc
    46441 actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag acaaggcgaa
    46501 cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact gatcgactta
    46561 ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg tggggttgat
    46621 gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac atccgttttg
    46681 aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc ttatttgaca
    46741 tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc ggaagcggga
    46801 aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt acagtccata
    46861 ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc atccgaaaaa
    46921 aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg gtagcaaaag
    46981 acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta gtcctagcaa
    47041 aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg ccatttgaac
    47101 tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg aggccggaag
    47161 tacttgtatt ggatgaacca acggcaggac ttgatcccca gggcagaaaa gaaatgatgg
    47221 agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt acgcatttaa
    47281 tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta
    47341 atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa aaacaactag
    47401 gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg aactttgcta
    47461 ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct ggaggaaatg
    47521 cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt gatccatcga
    47581 ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat ttttttagca
    47641 aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt cttcttgtca
    47701 aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct aatcctcttc
    47761 actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag ttggggtgtt
    47821 ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg
    47881 atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt atcggatgcg
    47941 attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga aatttcattg
    48001 atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga aaagatcatg
    48061 aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa
    48121 gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc ggaagatctt
    48181 gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa atatcgtgta
    48241 ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt aactgtgcta
    48301 ctgatctttt taagaggata aacgttcagt gggccgctat ctattctttt cttgctgatc
    48361 tgtttgcaag aaaggatgca agcagtgtat gacaagaaat attagggtct gtggcagact
    48421 aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa
    48481 tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac gttacaaagc
    48541 aatcattgcg tacgacggaa caaattttaa tggattccaa aaacagccaa atgggcgtac
    48601 tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag aaatcacggt
    48661 ctttggttcg ggaaggacag atgccggagt acatgcgatc gggcaagtaa tccattttga
    48721 ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc agtcaccaga
    48781 ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa gatatttggt
    48841 caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg
    48901 ccattatgca agttattttc cttacccact tgatttggaa aaaattcagc gggcgcttcc
    48961 tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct cgatagagga
    49021 taaaatacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag acgaattgtt
    49081 gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag taggaacgct
    49141 tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta ttgcgaaaaa
    49201 agatcgtaat gcagcaggac ctacagcaca ccctgaagga ctttatttgt atgaagtgaa
    49261 atatgaataa gaggttatga tagaagtggt cagcttcaag aaataagaag gtatttacga
    49321 aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaagaagct acttctggaa
    49381 caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa agagcaattt
    49441 caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc gttgataagt
    49501 gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac gttgttttga
    49561 ttgcccttgg tgcggacagt ctgaacaggt tccatcgttt ctttattttc tacgtcttct
    49621 tctgtgtgtg tttcgtggtt gttcttctat tgctggatca atcgtgccat aaaaggggcg
    49681 attgcggtca aggtaccaga cataaagaat aaacaatgca atacatagga caaaaaggat
    49741 tgcacctatc gtaaatcctt caggcaagta actgaaagtg acagtatgtt tacctgctgg
    49801 aacttttatc atcaagaatg cgtctttgaa attgctgacc ttgactggtt tcccatcaat
    49861 tttagctttc catcctttgt cataaggaat agttgtcacc aacatggaat cttcagcggt
    49921 atttacagtc ccagaagctt ttctgccagt ggcttccatt tcgacacctt tttctttgat
    49981 ctgattcatt gcggcttgat aggctacaac atctagacca acgactttag gctccatgaa
    50041 acttatttct ttcgtcccat aaaaacttgc agtaaatgtc actgttgtct cttgaggata
    50101 atagccaaga tcatagtatt gaccagaaat gttgatttgc gtttttcgcg atgtaccatt
    50161 gacagtgatc gtcgcactag aactttctaa ttgcgcaaaa ttggttggga acaaacttaa
    50221 gtaagcttgt gtatttgcag gcacatggac ttcccaagtg agctcttttg gctggttatc
    50281 tgctgtttct gagtaagtga cgccagtcgt tgtctttgtg atcgtcgtat tcaactgatt
    50341 cttcaaagta ggtgtataga atgtaaaata ctgttcattt agaccagaca atgtattgat
    50401 caagtttgtc tgactggtca aattatctgt cactggctgc tcaacatcat taacggacaa
    50461 cggtgcagta aatgctagtg gcaatgcatt gacattctga tacagtgtat agtcgccgct
    50521 ttgaccagct tggcggaagc catatttact gaacgtgctt tcgtttttag aaatgttata
    50581 tttgattcct gtaaacccat ccatcagcaa agtattattg ttataacgga tattcaaatt
    50641 tgtccctctt gaacggaaac ctaatttatc caggtaagag gaagaattac gatttcttat
    50701 agaagaaaac aaactgatac cactgtaacc atagttgatc gaatcgtttg cagatactgg
    50761 atccagattt tccaagcggt aaaacgagtc ggaatctttt tttgtttcat ttacaagagc
    50821 tttcaagtca ggataaggat cagtatataa actgcgggaa gcataattcc aatcatcaag
    50881 aatgccatgg atcatactgt tcgtattgac cagtgcttca gcagacatga tcaataataa
    50941 caatatactc agatgattga atgaaagttt tttatgctga taaaatccca cagcaaaagc
    51001 ataaatccct aagaaaacca ctgtcaatac aaaggaagtc atcgtcacat aggtgtagct
    51061 tccttcaggt ttgatgcccc atgccaccgc aaagagagca gctacgacca aaatgattcc
    51121 taacaacagc ccaccatcgt cttgttctat cttttcccat ccttttcctg caagcaggag
    51181 cactaagaaa gagaacagat aggcataacg gaataaaaac atattcggtg catgcatacc
    51241 atgccagaac aaattcaatg gaacaagata aaaacttgcg ataagtatgg cgaaaagact
    51301 gccgtacaaa aatttatttt tcaacggtac tttccgactg gcaaaataaa acagacagag
    51361 gactaatggt agcagacctg cgtaaacgaa cggaatcgat ccgtattttg tcgtgtcata
    51421 aacaccaatc atatttttca tgattagatc cagataagaa gtcgcttctg ttttcaacgt
    51481 agtgatctga ctcagtgcct cgccatttga ccgaagatcc agcactgctg gcaatacaat
    51541 gatcattgaa gcgccgcctg ccagtaaaga agtgatacca taaggaacaa tcgttttttt
    51601 atattctgac cagtttgtca atagacgggc gataaaatat aagaaagaaa atacgccaat
    51661 cataaaaccc atataaaaac tggataaaaa caagagtaaa taactgataa ataaaagttt
    51721 cggccgttta agatccataa cacgatgaat ccccaaaatc actaatggaa gataaacaaa
    51781 tgcatccagc cacatgatga tctccgaatg tgcagtgata aatgagatca atgcgtaact
    51841 gacgctaagt gcaacatgcc cccagcgagg aatcttgaac gtacgtgcaa gaaaccaaaa
    51901 agccaatccg gcacagccga ccttaagcaa agtcagaaca tacagtgcat ctggcatcat
    51961 ctggtttgga aaaaagaaca ccagtggcgt gaagatcccg cctagatagt aagaaattaa
    52021 agataaataa ttcaatccta gagaagcatt ccatgtataa aaaatacttt gctttccaag
    52081 aagaacattt cggaagctgg catgaaaatt agaaaactgt gaaaaggcat cacttgccaa
    52141 aatacttctg ctgcttccag gatagattcc gatacttaga taaacaagtg ctaaaatcaa
    52201 aaacggaatc aggaaactag ctgctgcgta gcgtccgttt tctctaaaga atgactttaa
    52261 attttttttc atatttattt tccaccaaat ttctatgaag ttgtattcag tttatcatat
    52321 ccttgttttt ctctgcttaa aaagagtcta gttttcacaa aaaaataaaa tttagaataa
    52381 ataacttcat aatttgttcg tataacaaaa ataaacgcaa aaaaagggta ggaatcaaaa
    52441 gtcccaccct taaaaatttt tattaaataa aaaaccgttc actatctgaa cggtccaagt
    52501 atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg acacccaaac
    52561 ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt taacagtacg
    52621 ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc gacctgtaaa
    52681 gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca atagaataat
    52741 tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac attttcatag
    52801 atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc tgaaattttg
    52861 caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta tgataaaata
    52921 tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg gctgtaaaaa
    52981 ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca gttgttggtg
    53041 gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa attaaagata
    53101 atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc cgccaagaag
    53161 ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa atctctgaag
    53221 tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta ggcgtaactg
    53281 ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag gcaggagact
    53341 ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta acagaaataa
    53401 gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa gtaaatgcag
    53461 aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga acaaatatga
    53521 atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa aaagtaggag
    53581 aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat tccggcgtta
    53641 ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc tcattaaatg
    53701 cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa gcagtaatga
    53761 aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct ggagaaaaga
    53821 aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa ggagcaaagc
    53881 gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt gtcgtagaca
    53941 gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg aatggagaat
    54001 tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg gtcaatgcag
    54061 aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt ggttactgta
    54121 cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc gattacgaag
    54181 aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat gatgacgaga
    54241 tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat ggacaaaaat
    54301 tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag acgattttgg
    54361 aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc ccgtatggta
    54421 tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta ggcgctaatt
    54481 atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt aaagcgattc
    54541 aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc ttcatggcag
    54601 ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca agaacgattt
    54661 cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa gaaaacaaag
    54721 aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac gcgatcagag
    54781 atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg atcgatggaa
    54841 aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta gaacaaatga
    54901 tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca gttgaacaag
    54961 caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc gagatccacc
    55021 aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg tgtcagaata
    55081 tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg tatgatcgct
    55141 gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt tagatgatcc
    55201 aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca gcttgggcat
    55261 ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata ttcaggaaag
    55321 aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg tagtctcgcc
    55381 tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta tgatgcaaga
    55441 acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa aagtcatttt
    55501 atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct tgaacgggat
    55561 gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg tcaacaaatc
    55621 aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat acggcacatt
    55681 gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg accgagcttc
    55741 tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg
    55801 tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga aaaaagcaga
    55861 aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga aacaatttac
    55921 tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg
    55981 ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag gtagcggaaa
    56041 aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt
    56101 gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac agctgtttga
    56161 tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag aacgacgtga
    56221 gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac atgcattgat
    56281 ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc agcaccgttt
    56341 tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg tcttatttat
    56401 gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga tggatgtatc
    56461 tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg taagaacacc
    56521 tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac atcaaatgta
    56581 tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg cagtggaaat
    56641 ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt tacatggtaa
    56701 aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc agatgcagat
    56761 attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga ccgtcatgct
    56821 gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg gacgtgttgg
    56881 acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg aactaggtgt
    56941 ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg aaaaagatct
    57001 ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc ctcaatttgc
    57061 gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag aggctgcagc
    57121 cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag cgcaaagcct
    57181 ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat actttatact
    57241 gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg gcggagatca
    57301 cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc cagaagtaga
    57361 attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg aaaaaaacat
    57421 cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa aagcaattcg
    57481 acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg gtgaagcaga
    57541 tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt tcatcgtagg
    57601 acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag tagggaaaga
    57661 aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac cagaacattt
    57721 gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta tcggacgtcc
    57781 tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag tgacgaaaaa
    57841 agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg tagaagctcg
    57901 tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag gcaacgctgt
    57961 tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat ctgctattct
    58021 aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct tacgttcttt
    58081 gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct tgaaagcacc
    58141 agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga tccgccaaat
    58201 ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg aaaaaagcga
    58261 agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg gacattcctg
    58321 aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga ttcttggagg
    58381 tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt tcaaatcatt
    58441 ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat ttaaaagctg
    58501 attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt acggaaattt
    58561 cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtagattat atcagcagcc
    58621 atcttaaata atttcaaata aaagcaaaga aggataaaaa gctgcatgca gctttttatc
    58681 cttctttttt taatcttttt gacacgtaaa agtcattttt ctgataattc tggcgataaa
    58741 cagaaattat gttgcaaagt aaagcggttt tctatataat tattaataat tcattacaca
    58801 aagataagaa taacctttgt tataaaacga tttattctgt gtaatgtcct tttttattaa
    58861 gaaaaacttc catttaggaa gcagagaggg gtattcgatt gtccgataac caattattag
    58921 aagttcagaa cctacataca ggattccgac tcaaagatga ctattacgat gcagtagatg
    58981 acgtttcatt tactttagac aaaaacgaga ttttagcaat cgttggggaa tctgggtgcg
    59041 ggaaaagtac cttggctact acgattatgg gattacttga tcctaataac acaaaaatta
    59101 caggtgagat tttatataac gatctgaatt taatcgacct gaacgaaacg ctctataata
    59161 aaatccgcgg aaatgatatc ggaatgattt tccaagatcc gttgtctgca ttaaatcctc
    59221 tcatgagaat cgaagaccag atcaaagaag gactttccta tcacacaaag atgaatgcag
    59281 aacagcgtca agctcgtgca ctagaattgc tagaacaagt agggattcct aatcctgcga
    59341 gagttggacg ccaatatccc catgaactat caggcggtat gagacaacgt gtcatcatcg
    59401 caatcgcaat cgcctgcaaa cctccaatcg tcattgcaga tgaaccgacg acagccctag
    59461 atgtaaccat ccaagcacag attcttgatt tactgaaaga ccttcaggaa gaaacaaaaa
    59521 cagggatcat cttgatcact catgacttag gagtcgtagc agaaatggct gatcgagtgg
    59581 cagtgatgta tggcggacag ttcgttgaag tagcaggtgt aaaagaactg tttgatcatc
    59641 ctaagcatcc ttatacacgc tctttactga actctatacc acaagaagga aatcatgatg
    59701 cagaactcca tgtgatcgaa ggtgtcgttc cttctctgaa aaacatgcct cgtaaaggtt
    59761 gtcggtttgc accacgtatt ccatggatac cagcaagcga tcatgaagaa gagcctatcc
    59821 ttcatgaagt cgcaccgaac cacttggtac gttgctcctg ctataagcat ttccacttta
    59881 gagacgaaaa aggagaggtg tagatgacag aattaatcac aatcaaagac ctgaaagttc
    59941 attaccctat tcgcagtgga ttcttcaatc gagtaacgga tcatgtatat gctgtagatg
    60001 gtgtggattt tattatcgaa aaaggaaaga catatggttt agtgggagag tctggctctg
    60061 gaaaatctac tacgggaaaa gccgttgtgg gattagaaaa ggtaacagcc ggacaaatca
    60121 tgtatgaagg aaaagatgta acgaagcgaa gcaaccgcaa aaaaatgggt tataacaagg
    60181 atgtgcagat gatcttccaa gattcaatgt ccagtttgaa tcctaaaaag cgagtgttgg
    60241 atatcattgc tgaacccatc cgaaattttg aacggttaag cgatcaagaa gaaaagaaaa
    60301 aggtaaaagg actgctagat atcgttggga tgccggaaga tgcgctatat aaatatcctc
    60361 atgaattttc aggcggacaa cgtcaacgtc taggagtagc ccgtgctgtt gctaccaatc
    60421 caaaactgat cgtagccgat gaacctgtat ctgctctgga tctatctgtt caagcgcaag
    60481 tattgaattt catgaaacga atccagcaag aatacggcct cagctattta ttcatttcac
    60541 atgatttagg tgtagtaaaa cacatgtgcg acaatatcgc aattatgtac aaaggtcgat
    60601 ttgtagaaat cggtacaaga gaagacattt ataatgaccc gcgacatatc tatacgaaac
    60661 gtctgctttc agctattccg caaatcgatg tagaaaatag ggaaagccac aaagaaaatc
    60721 gacgaagagt agaacaagag tatatccaaa accaaaaaga atactatgat gcgactggtc
    60781 gagtctacga tttgcgtacg atcacaacta cacacaaagt ggcgttgaaa gatggaggtg
    60841 caagctaatg tggaaaacga ttctgcgacg tgttttattg atgatcccgc aagttattat
    60901 tttaagtgtt ctgatcttct tattagccca agcgatgccg ggggatccat ttaccggttt
    60961 gatcaatcca aatcaggatc cggcagtgat cgaacagatg cgacaagcag ctggcttgaa
    61021 tgacccatgg tatgtccagt acttccgttg gatcggaaat gctttgcaag gcgattttgg
    61081 tcaaagtttc ttatacaaac gctctgttgc tgacttgatc ggcgaacgga tcatcaacac
    61141 tttatatcta tcgatcttga cagttgtcct tacttatttg attgctgtgc ctctggggat
    61201 gcttgctggc cggtatcaaa attcaatctt ggataagatt gtcgtaatct acaacttctt
    61261 tagttttgct gttcctttat ttatcttcgg cttgatcatg ctatttgttt ttggttatcg
    61321 tttaggctgg ttcccaacaa gtggatcaca aacactaggt ttgcaaggtg gatttatcac
    61381 tcaatggata gaccgtttga aatatattct gttaccatca attacccaag catttttggc
    61441 gactgccgtc acaatccaat atctgcgaag tgaagtaatc gattcgaaat cgctcgattt
    61501 tgtcagaaca gcccgttcca aaggggtacc gacgaataaa gtattcagtc gacatatttt
    61561 ccgaaatgca gcgttgccga tggcttctca aatggggtat gaaatcactt cgttgattgc
    61621 tggttctgta gttatcgaaa agatttttgg ttatccggga gtcggaaaac tatttattga
    61681 ttccatcggc caacgggact ataccgtgat cacatcctta gtattgattt taggaatcgc
    61741 gacgctagtg gggacattac tttcagatat catcatgagt atcgttgacc cgcgtattcg
    61801 gattcaataa aaccaatcag aaaggagaat ggatcgatga gtgaaaaaaa caatacagca
    61861 gttcaagaaa gtattcctcc tatggggata cgaatgattg ctcgagaatt taaaaaagat
    61921 aaaattgcta ttttttcatt aggtttgcta gtggttttat tattagtgat ctttatcggt
    61981 gcgatgctga ccgatcaaga caaagtgatg tatgttagta tcttcgacaa atacgctgca
    62041 ccaggaacag tttcgacaca aggacaaaag ttcattctgg gagcagatga aggcggacgt
    62101 gatgtattag gacaattgat cataggtgct agaaactctg ttacgatcgg atttgcgatc
    62161 acgatcctta cttcgatcat cggggttgga ttagggatca tctctggcta ctacggaggg
    62221 atcattgaca acatcttgat gcggatcgtt gattttatca tgattctacc gatcatgctg
    62281 atcattatcg tcttcgtatc gattatttcc agatacaatg tctggtcctt tatattcatc
    62341 atgagtgcat tttattgggt agctaaagcc cgattattcc gaagcaagac attatctgaa
    62401 gcaagaagag attatgtcag tgcctcaaaa acaatgggaa ccaatgattt taaaatcatg
    62461 ttccgcgaaa tcatgccgaa tctaagttca ttaatcatta ctaatttgac gatgaacttt
    62521 gcggcaaaca tcggtatcga gacaactttg actttcttag gtttcggctt gccggcaaat
    62581 gtacctagtt taggtacatt gatcggttat gcaagtaacg gagaagtctt agtgaatcgg
    62641 caatggatct ggttacccgc atcaatcctt attttagttt tgatgttgag tatcaattat
    62701 gttggacaag cattcaagcg ttctgcagat gcgcgccaac ggttaggcta gtatagaagc
    62761 gaagggggaa ataggaatga agaaaacgat ttttggattt gcagcgctga tgtctgctgt
    62821 cgttcttgca gcatgcgggg gcggaggtac agatacctct ggaaacaact ctggaggcaa
    62881 cggcaataac aaagaggaat taacttttcc aattgcaaca aaaaataatg atgatgcaat
    62941 cgaagacggc gaattagaag ctgcggtcgc aacggacagt cagtttaaag gattgttcca
    63001 gtgggagttt taccaagatt catatgatgc agcttttatg gctccttctc atgaaccgct
    63061 atttgcaaac gatgacgatt tcagaatcac agacgacgga gctgcaacgc ttgatctaga
    63121 tgaagatacg aaaaaagcga caatcaaagt aaaagacaac gtcaaatggt cagatggtga
    63181 agatgtaacg gcggacgatg tgattttccc ttacgaaatc attgggaata aagattacac
    63241 tggtatccgt tatgacgaca ctttccgtaa catcgtaggt atggaagaat atcatgatgg
    63301 aagtgccgac acaatctccg gaatcaaaaa aatagacgac aaaacagttg aaatcgaata
    63361 taaagaagta aacccaagca tgcttcaatc gggtggtggt atctggtcat atgcagcacc
    63421 aaaacatacg ttagaaggtg tagcaatcaa agatatggaa tcaagcgatc aagtacgtaa
    63481 aaatccagtg acttttggcc cttattacat gagtaatatc gttgcaggag aatcagtaga
    63541 atacttgcca aacgagcact actggaatgg tacgcctaaa ttgaaaaaga tcacgatgaa
    63601 atctgtaccg atggcaagtg caactgaagc attgaactct aaattatatg atatgatttt
    63661 ccaaatgcca acagatactt acgataccta taaagaaatt gctggctata caaacttagg
    63721 ccgtgaacaa acttcttata cttatctagg attcaagtta ggtaaatgga atgcagatac
    63781 aaagagtgtg gaatacaatc cagattcaaa aatggctgat aaatcattac gtcaagcaat
    63841 gggctatgca ttagataata atgcagtagg tgaacgtttc tatgcaggtt tgagaagcaa
    63901 tgcgacttca ttgattcctc cagtattcca gtcataccat gatgatgaga tgaaaggatt
    63961 cacacaagat ctagacaaag cgaaaaaact attagatgat gctggctaca aagatgtgga
    64021 tgacgatggt ttaagagaag acaaagatgg aaataaacta acaatcaact ttgcttcgat
    64081 gtccggtggg gaaacagcgc aaccattggc agattactat atccaacaat ggaaagaaat
    64141 tggtttggat gtccaataca caacaggtcg tctgatcgaa ttcaattctt tctatgataa
    64201 attagaaaac gatgatcctg aaattgatgt ttaccaagct gcttggaata caggaactga
    64261 tccaaatcca agtggactat ggggaccaaa tgctgcgttc aactacactc gttatgaatc
    64321 agaagaaaat acaaaattga tcaacgatat cagttcaagc aaagcatttg atgaagagta
    64381 ccaaaaagaa gcatatcaaa aatggcaaca atatgcattt gaagaagcat ttgcgattcc
    64441 aacgttgttc cgtaatgaag tattgccagt caacgaccgt gtcaaagact gggattggtc
    64501 ttatgatgta ccaaacccat gggcaaatgt ttctgttaca gcagattcac gttcataatc
    64561 actaaaaatg accgaaaaag ggcgatgtcc tttttcggtt ttttgatatt tttctgttta
    64621 tttctctaat attcaggtca aggcgctgat ttcatgactt ctccaataaa cggtgttcaa
    64681 agtccagttt ctcggtatta agtcaaattt atgcaaaaat atggaaagcc attttcacaa
    64741 aattttttct tcatactcga aactaaacga atttttcaca acttctcgtc aatcactagg
    64801 attattaaag ctttacttac cttttgaccg aaccatttca aatttgacaa aaaaccagta
    64861 taataaggaa gtatcgaaaa aatatccata aaaggaatgt ggaaaatgga caaccagctg
    64921 acaaaagaac taaaagaaaa ctataacatc gtttttcacg atctaaattt gcttgaacaa
    64981 gcttttaccc attcatccta tgtgaatgag catcgcaatc tgcaattatc ggataatgag
    65041 cgacttgaat ttttaggaga tgccgtttta gaactgatgg tttctcagta tttattccgt
    65101 ttatatcctc atttgccaga aggaaaattg acaaaaacac gagctgcgat cgttcgcgaa
    65161 gacagcttgt cgaaatttgc caaagaatgt cactttgatc aatatgtttt gttgggcaag
    65221 ggagaagaga attcaggagg acgtacgcga ccagcattgc tttgcgacct attcgaagct
    65281 tttttaggcg cgttatatct tgaccaggga ttcgacacga cccatgcatt catcgaaaaa
    65341 gtgatttttc ctaaagtcaa agcaggtgct ttttcacatg agatggatca taaaacaaaa
    65401 ctacaagaag tcttgcaaaa atcaggtgat gtatcaatcg aataccgatt gatcaatgaa
    65461 gaaggtccag ctcatgaacg agtattttgg atcgaagttt atgtagatga tcaattgatt
    65521 ggcactggac aagggaaatc gaaaaaatta gcagaacaag ctgcggcgga aaatgcattg
    65581 gcagcactgt aaaagtaagt gagggaaagc tttgtatttg aaacgaattg aaatagccgg
    65641 ttttaaatca tttgcagaca agacagtcat tgattttgaa aatagcgtca cagcagtggt
    65701 tggaccaaat ggcagcggaa aaagcaatat tacagaggct atccgctggg tattaggaga
    65761 acaatctgct aaaagtctgc gaggcggaaa aatgccggat atcatctttg ccggttccga
    65821 tacccgtaag cagctgaata ttgcagaagt cacagtgatt ttagacaata gtgatcatta
    65881 tctgccattg gagtacaatg agatcagtgt aacccgccgt tatcgacgga ctggggaaag
    65941 cgagtttttc atcaataaac aaagctgcag attaaaagat atccaagaat tgttcttaga
    66001 ttctggatta ggaaaagaat cgttctcaat catttctcaa ggaaaagtag aagcgatttt
    66061 cagtagtaaa ccggaagacc gtcgtgggat ctttgaagaa gcagctggtg tcttgaaata
    66121 caagcagcgg aaaaagaaag cagaacagaa gctttttgaa acagaagaca acctcagccg
    66181 cgtccaagac atcatccacg aattggaaga acagttaact cctttggccg cccagagtga
    66241 ggcagcaaag gagtttttac ggctaaaaga aacactgact caaaccgatg tctctttgat
    66301 ggttgctgaa atcaagacag caaaaaaaga ttgggacaac aaacaagcac agcttgcgaa
    66361 attcaatcta gaattaggca aactatctga gtcgatccaa gaacaagaat ccattttggc
    66421 gaagcagcgt aaagaaaatg cgcaagccga tcgtctgatc gaaaaaaatc agcaagtatt
    66481 attagatctt tcggaaaaac tgaagcagac agaaggacaa aaagatgttt tgcaggagcg
    66541 gacaaagcat acacaaaaaa gctcgcaaga atatcaaact tcattagcag aagcacaaaa
    66601 aaaggttaaa cattttgaaa agcttcaaga aagcttgatg aaagcagcag cagaaaaaga
    66661 aacagagatc caaaaagctg aagcaaatct cataaaaaca cagcaagaat tagaaaaata
    66721 ccaaaaatca acgaaagaat tattggcaga attaagagat caatatgttg atttgatgca
    66781 agaacaagca gctgtaggaa acgaactgaa gtatctagaa cgtcaatata tacaagaaac
    66841 agcaaaaagc aagcagacgt tagccaagca atcggaagta gaagcatcag tagaccgatt
    66901 gatcttacaa aaagaagaat tgacacaaaa gcaggcgcag ctaaaatcat ctttaactga
    66961 aacaaaagag aagttagaaa tgatccaaca aaacgggaaa aagttccaag aagcattagc
    67021 aaaagaacag cctaaaatgt atcaattaat gaatcaagtc cagcagctac gtgctcgtaa
    67081 gaaaagcctt caagaaatcc aagaaaacta ttttggtttt taccaagggg ttcgattggt
    67141 cttgcagcat aaacagcagc tttcgggtat tgtcggagca gtggctgaac tgatcgatgt
    67201 tcctgcagat ttcacgttag ctatcgaaac agctttaggt ggggcagccc agcatgtcat
    67261 cgttgaaaat gaaaaagatg ctcggcaagc catcacgtat ttgaaacagc aaagaggagg
    67321 aagagcaaca ttccttccac tgacgacaat caaaccaaga caattaccgg ctcatatatt
    67381 gacacaagca gcagctgtag aagggtttat agggattgcc agtgaacaag tcagttatcc
    67441 cgatcaaatc caaactgtgg ttcaaaacct gctaggaacg atccttttag ccaaagactt
    67501 aactagtgcc aatgcaatcg cgcaaacgat ccgctatcaa tatcgagtgg tttccttaga
    67561 aggtgatgtg atgaatgccg gaggatccat gacaggtgga gcgaataaac gaggaaacca
    67621 aggcagtttg tttgtacaaa atcaagaatt gaaacagttg actagtgaat ttgaagaagc
    67681 agataaacaa ctccaagcac aagaaaaaaa agttcaggag ctgcaacaag aaacagctcg
    67741 tttagccgaa gaacaagaag ttttgcgtac acgaggcgaa caattacgtt ttgaagaaca
    67801 agaagcgaca aatcagctac aaaacatcat aaatgaactt gaacgttttg aaaaagaaaa
    67861 gcaaatctca acttttgaga cacgtgaact gcagcagttt atcgaagatt acgaaaagca
    67921 aacgaacgaa ttgaaagaca aacaaacaga tcttgaatcg caacgacaaa aaatcgacga
    67981 agaaatcaaa tcgttaagtc aagaaagcga ccagatggaa gccagaagag cacaagtaca
    68041 aagtcaaaaa gcacaagaac aagcagatct tgctgtcttg aaagagcagt ttaatcatct
    68101 gcagatccag ctccgcggtg cccgagtcca aaaagcagag gcaaccgaac gtcaagaagc
    68161 gatagaaaag cagttggcga cattgacagc agacttttct gatcatgaag tcacagaaga
    68221 aagtttggaa aaacaaatta acgaattatc tgctcagcga gaaacattaa aagccgagtt
    68281 ggcaaaagcc aaagagcagc gagatagaag gcaaaaggag atcgataagc ttgaagcagt
    68341 cttggcagag cgtaatcgtg agcaaaaagc acgactgtcg gaacaatcca aattagaagt
    68401 ccaaaaagac cgagctgaaa tgattttaga taatcatttg acctatctgc agtcagaata
    68461 tcagctgaca tttgagaaag cttcccaaga ctatcaagaa acaaccgata tcgaagacag
    68521 tagaacaaag gtttcatcct taaaagaaca gattgaaaaa ctgggaccag taaacttaaa
    68581 ttcaatcgaa caatacgaac aagtcagtga aagacataca ttcttagcaa cacagcgaga
    68641 tgatctgtta gcagcgaaaa accaattgtt tgaaacgatg gatgagatgg atgatgaagt
    68701 gcggacaaga ttcaaagaag tatttgaagc tatccgtcaa gaattcaaaa ttgtctttcc
    68761 aaatatgttt ggcggcggta aagctgaatt agtactgacg gaaccaactg atttgctgaa
    68821 aacaggtatt gagattgaag tacaacctcc tggcaaaaaa ctgcaaagtc taagtttgct
    68881 ttctggagga gaacgagcac ttacagccat cgcgttgctc ttttccatta tccgtgtctg
    68941 tcctgttccg ttttgtatct tagatgaggt agaagcagcg ttagatgaag ccaacgtgaa
    69001 acgttttggt cgttacttga gcgatttcca agatgacaca caatttatcg tagtcacaca
    69061 ccgcaaagga acgatggtcg cagcagatgt actgtacgga gtgacgatgc aggaatccgg
    69121 tgtatcaaaa atcgtttctg tacggatgga agatatcaat gaagaggcga aaatcaacga
    69181 aacgaaagtg taggaacgaa aatgatcaaa ttagtagcaa tcgatttgga cggtacatta
    69241 ttaaatgaac aaaagctgat ttcagatgag aataaacaag cattagcaca agcaaaacag
    69301 caaggggtca agatcgttct atgtacagga cgccccttag ctgcgatggc gcattatctg
    69361 caagaattag gactggtaga tgaaggtgac tacagcatca ctttcaacgg cggtttggta
    69421 caaaaaaatg atactggtga aatcatggaa aagaaagtga tggaagtatc agatatccaa
    69481 cgcttataca aactggctca gcaattggat ctgccactag atgttttgtc tgacaatgtg
    69541 gttttacagc tgcctagtgc cccaaggaag caatccttgt acaatgtgtt gaataagctg
    69601 ctgcaattcc aaccagcgga tataagcgat attacagatg aatttgtgct taataaggca
    69661 gtaatcgctt atgaccaaga agaattagat cctaaaatca aagaaatacc agcagaattc
    69721 catgaaatgt atgagatcat caaaacaaga agtatgcttt tggaatttat gccaaaaggt
    69781 gttacaaaag cttatggtat ttctttattg gcaaaagatc ttggtttgaa acaagaagaa
    69841 atcatggcga tcggtgatga ggaaaatgat ttaccgatga tccaatatgc tggactaggc
    69901 gtagcgatgg agaatgctgt gcctttcgtc aaagaagcag cagatttcgt gacagccagc
    69961 aatgaagaaa atggcgtagc caaagtgatc caaaaatttg tattagagta gggggagtca
    70021 aatgggattt tttgataaaa tcaagcgtgc ttttactggc gaagagaaaa cagaagaagt
    70081 tgaagtacag gagaagtacg acaagggttt agaaaagacg cgtaaaacat ttggtgaacg
    70141 gatgaatgag ttgtttgcca attttcgaac agtcgatgaa gacttttttg aagaattaga
    70201 agaaacactt atcggtgcag atgtaggttt tgaaacagcg atacgaatta ccgaagcatt
    70261 acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca gaagtccaaa atgcgatcat
    70321 cgagaaactt gtggatctct atgaagcgga aggaatcaat gaagtaaatg aactaaatat
    70381 ccaagaagac ggactaacag ttattttgtt tgtcggtgtt aatggtgtag ggaaaacaac
    70441 aagtattggt aaacttgccc atcagttcaa aaacgaaggt aaaaaagtat tgatggcagc
    70501 agcagatacc ttccgcgctg gagcaatcga ccagttagtt gtctggggcg aacgcgctgg
    70561 tgttgaagtt gttcgtggca atgcaggagg cgatccagca gctgtcgtat tcgatgcttt
    70621 ggaaagagca aagacagagc aggcagatgt tttactagtt gatactgcag gccgactgca
    70681 aaataaagtg aacttgatga acgaattaga aaaaataaaa cgagtaatcc agcgggaatt
    70741 accagaagca ccccaagaaa cacttcttgt attagatgca accactggtc aaaacgcgat
    70801 ggttcaagca aaacaattca aagaaacaac agatgtgacc ggtcttgttt tgacaaaatt
    70861 agatggtaca gcaaagggcg gtatcgtact tgctatccgt aatgaattgc atttaccagt
    70921 gaaattggtc ggtcttggtg aaggaatcga tgacttggag ccgtttgatc caaatgattt
    70981 tgtcgttgga ttgttcaaag gattactaaa agaagaataa cccatgtcta aaaagcagga
    71041 gctgtgacaa aagttgcagc tccttgtttg aatgattgtt tttaacatag ctgtgttttt
    71101 gatgccttca tttttatact tatttgtaag cggttctcaa acaatttcgt tctttttctt
    71161 agactttttg attataatta gattcatatg aattggaggt cacttaatga tgaattttaa
    71221 gcaattatta ttgcatgtca atgcgcgccc ttttatcgac caagctcgat ttggtataga
    71281 acgggaagga cagcgggttg atcttgcagg aaatctagca aaaaccgatc atccagcaat
    71341 ctttggcgat cgatcctatc acccctatat ccaaacagat tttagcgaaa cacaaacaga
    71401 gatgatcacc cctgttaccg attctattcc cgaattattt cagtatctcg ctgctgttta
    71461 tgatgtgact gctcgttcta taccgaaaga agagatgatc tggccattaa gtatgccacc
    71521 tgccttaccg gaaaaagacg aagagatcat tattgcaaaa ttaaaaaatt tcgaagatgt
    71581 cttgtatcga cgttatttag caaaagaata cgggaagcga aaacagatgg taagcggtat
    71641 tcatttcaat tttgaatttg gcgatgagtt gctaagaaca ttgttcagcc atcaggaaga
    71701 atttcaagat ttttccgaat tcaaaacaga actttatttg aaaacagcga gaaactttat
    71761 gcgctatcgc tggatgatca cttatttatt cggtgcttca ccaatgagtg agaagaacta
    71821 ctttttagat gaatcacatc cgcaagaacc tgttcgcagt attcgcaaca gtgcgctggg
    71881 ctatacgaat catccaaatg tgaaagtttc ctatgcttct atgaaacagt atttagcaga
    71941 tatcgagcga atgatcgaag aagggaaact ttcggaggag aaagaatttt atacaccgct
    72001 tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca acaggtgttc gttatattga
    72061 attgcggaat atcgatttga atccttatgc aagattgggt atcaatccag agcaagttcg
    72121 gttcttacaa ttattcctga tgtacatgtt atggacagaa gaaaaagaag actgtgacca
    72181 atgggtagca gaaggtacga ctcgaaataa caaagtagca ctagaacaac catcagatca
    72241 aacagaattt catcaagaag gtagagaaat tcttgaagga atgaagcaaa tgctggttga
    72301 attggattgg ttggattctc tttatttagt agaggaagca ttgactcaaa tggatcatcc
    72361 tgaacaaacg ttagcagcga aactctatca agaagcacag ctatcgagtc agcaagaagt
    72421 tgctgtggca ttgggacatc aatattataa agaaagccat gaacgtccct atcagttggc
    72481 cggttttcgc gaaatggaac tttctactca gatatttatg ttcgatgcca tccaaaaagg
    72541 tgtccaagtc aaagtattag atgagtcgga tcaatttttg cgtctgcaat tccaagacca
    72601 tgtagaatat gtgaaaaatg caaacatgac aagtaaggac agctatatcg tcccacttat
    72661 catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa gctgggttcc gggtaccagg
    72721 aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct tatccgagat ttgccgagca
    72781 ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta ggtatcacga ttttcaagga
    72841 aggagctagt ctggaagatt atcaagcagg tttagccatt gcttttcgtg aagacagttc
    72901 ggttttagta gaagaattta tgccgggaac ggaatatcgt ttctttgtga tcgacggaga
    72961 agtccaagcc atcatgttgc gagtccctgc taatgttatt ggtgacagta tccgaacagt
    73021 gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt ggaacgaatc atcgtgcacc
    73081 tttagaattg atccaattag gtgaattaga acaattgatg ttgaaagaac aagggttgac
    73141 gatcgaatca gttccccaag ccaatcaaat tgtttatcta agggagaact caaatatcag
    73201 tacaggtggt gattcgatcg atatgactga tgaattttct gaagcttaca aaaaaatcgc
    73261 cgtttctgcg gtagaagcac taggagctaa gattagtggt atcgatttga ttataccaga
    73321 taaagaaatc gatcctacaa ctgataaaaa agcatatggg atcatcgaag caaatttcaa
    73381 tccagccatg catatgcatg tttatccttt tgcaggaaaa ggcagaagat tgacgatgaa
    73441 tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa aagcttttga cagattgtcc
    73501 tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg tgttttttcg
    73561 ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt ttcagctggc
    73621 gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt tactgcttct
    73681 tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc gttgactaca
    73741 gtgaaacggc tattgttttt cgttgctagc tggatatatt ggttttggac agaaaggggt
    73801 agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt taaaaagttt
    73861 tctttgtttt ttggttggat catttcagca acagtcatac taagatagca gcgttccctg
    73921 aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc ataaagtccc
    73981 ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct tcttctcttt
    74041 gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa aaagcggttg
    74101 cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta gaaaggtgga
    74161 ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta tttcattgtt
    74221 catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc aaagcttaca
    74281 aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg gagcgaccag
    74341 tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt caccaacgac
    74401 tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt tcctacctgg
    74461 tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag gaacattgct
    74521 ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc cgttgattgg
    74581 agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc aattccagtt
    74641 ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac gaggaaatta
    74701 tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg cttaccgatt
    74761 tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt accagaagat
    74821 gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga tcatggtagg
    74881 aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat atggagatca
    74941 agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag tattttgcga
    75001 tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga tcgttggaat
    75061 catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg cttaaaaaaa
    75121 agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga atgtacttga
    75181 cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag aaaatttttg
    75241 catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag acaattacgc
    75301 ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc cttgtccaaa
    75361 cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt acagtttttt
    75421 tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc tttcttttga
    75481 aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta ggtacgcttt
    75541 ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat gatttgcttc
    75601 aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg cctgcttctt
    75661 tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta gacgttttcc
    75721 atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt gaatttttga
    75781 ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt ccagttactt
    75841 ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat cgtcatggtc
    75901 agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga cagctcgcag
    75961 tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa
    76021 tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg atttacaaaa
    76081 ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa cgattcctga
    76141 aaagaaattg actgctttta tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat
    76201 gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata acgaagtaaa
    76261 ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat cgaatgaaat
    76321 aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt atacgaaagg
    76381 aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacacc ttgggttaga
    76441 agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac gtgcgtatat
    76501 tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt tgaacaatct
    76561 agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac aagaaatcgg
    76621 gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg cagacgaaat
    76681 tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa tgataaaaaa
    76741 agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg aaggccgcag
    76801 attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa ttagtagtag
    76861 gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac aacaccagtc
    76921 aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata acagatagta
    76981 atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc gacaatggtt
    77041 ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca gctacaagca
    77101 gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga ataaaagaac
    77161 cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct ggccgacaaa
    77221 gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct ttagatgcga
    77281 aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc tttggtaagc
    77341 aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag acgaaaaaga
    77401 aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg caattatcag
    77461 cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt gaacaagcag
    77521 atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat gtgctggaaa
    77581 aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga gacgacagca
    77641 aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa aatcagcaga
    77701 tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa tcaatgaaac
    77761 tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg attaaactct
    77821 catactatgt aacaataatg tttcttacat tcttaaatag caagagccta tcctcaaaga
    77881 agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt tttattt
//
LOCUS       DAPCMU010000002        68366 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPCMU010000002 DAPCMU010000000
VERSION     DAPCMU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 68366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.002"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            28..909
                     /locus_tag="RD744_000089"
     CDS             28..909
                     /locus_tag="RD744_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556126.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            922..2532
                     /locus_tag="RD744_000090"
     CDS             922..2532
                     /locus_tag="RD744_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDU0556127.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2609..3268)
                     /locus_tag="RD744_000091"
     CDS             complement(2609..3268)
                     /locus_tag="RD744_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDU0556128.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3551..4216)
                     /locus_tag="RD744_000092"
     CDS             complement(3551..4216)
                     /locus_tag="RD744_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDU0556129.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="RD744_000093"
     CDS             complement(4363..4968)
                     /gene="dhaL"
                     /locus_tag="RD744_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDU0556130.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="RD744_000094"
     CDS             complement(4996..6006)
                     /gene="dhaK"
                     /locus_tag="RD744_000094"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDU0556131.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5981..6427)
                     /gene="dhaM"
                     /locus_tag="RD744_000095"
     CDS             complement(5981..6427)
                     /gene="dhaM"
                     /locus_tag="RD744_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDU0556132.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6530..6850
                     /locus_tag="RD744_000096"
     CDS             6530..6850
                     /locus_tag="RD744_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDU0556133.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6962..7831)
                     /locus_tag="RD744_000097"
     CDS             complement(6962..7831)
                     /locus_tag="RD744_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0556134.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7927..9230)
                     /locus_tag="RD744_000098"
                     /pseudo
     CDS             complement(7927..9230)
                     /locus_tag="RD744_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9227..9736)
                     /locus_tag="RD744_000099"
     CDS             complement(9227..9736)
                     /locus_tag="RD744_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556135.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9911..12046)
                     /locus_tag="RD744_000100"
     CDS             complement(9911..12046)
                     /locus_tag="RD744_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0556136.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12129..13784)
                     /locus_tag="RD744_000101"
     CDS             complement(12129..13784)
                     /locus_tag="RD744_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0556137.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13786..14100)
                     /locus_tag="RD744_000102"
     CDS             complement(13786..14100)
                     /locus_tag="RD744_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDU0556138.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14270..14530
                     /locus_tag="RD744_000103"
     CDS             14270..14530
                     /locus_tag="RD744_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDU0556139.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14544..14858
                     /locus_tag="RD744_000104"
     CDS             14544..14858
                     /locus_tag="RD744_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDU0556140.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15588..16142)
                     /locus_tag="RD744_000105"
     CDS             complement(15588..16142)
                     /locus_tag="RD744_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDU0556141.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16268..18196)
                     /locus_tag="RD744_000106"
     CDS             complement(16268..18196)
                     /locus_tag="RD744_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDU0556142.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18502..19218)
                     /locus_tag="RD744_000107"
     CDS             complement(18502..19218)
                     /locus_tag="RD744_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDU0556143.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19219..21042)
                     /gene="glpO"
                     /locus_tag="RD744_000108"
     CDS             complement(19219..21042)
                     /gene="glpO"
                     /locus_tag="RD744_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDU0556144.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21056..22552)
                     /gene="glpK"
                     /locus_tag="RD744_000109"
     CDS             complement(21056..22552)
                     /gene="glpK"
                     /locus_tag="RD744_000109"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDU0556145.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22688..24148)
                     /locus_tag="RD744_000110"
     CDS             complement(22688..24148)
                     /locus_tag="RD744_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556146.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24138..25433)
                     /locus_tag="RD744_000111"
     CDS             complement(24138..25433)
                     /locus_tag="RD744_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0556147.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25765..26442)
                     /locus_tag="RD744_000112"
     CDS             complement(25765..26442)
                     /locus_tag="RD744_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDU0556148.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26472..27563)
                     /locus_tag="RD744_000113"
     CDS             complement(26472..27563)
                     /locus_tag="RD744_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDU0556149.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27664..28098)
                     /locus_tag="RD744_000114"
     CDS             complement(27664..28098)
                     /locus_tag="RD744_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0556150.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28682..30139)
                     /gene="ascB"
                     /locus_tag="RD744_000115"
     CDS             complement(28682..30139)
                     /gene="ascB"
                     /locus_tag="RD744_000115"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDU0556151.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30136..32031)
                     /locus_tag="RD744_000116"
     CDS             complement(30136..32031)
                     /locus_tag="RD744_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0556152.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32089..33864)
                     /locus_tag="RD744_000117"
     CDS             complement(32089..33864)
                     /locus_tag="RD744_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDU0556153.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33914..35410)
                     /locus_tag="RD744_000118"
     CDS             complement(33914..35410)
                     /locus_tag="RD744_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0556154.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35604..37226)
                     /locus_tag="RD744_000119"
     CDS             complement(35604..37226)
                     /locus_tag="RD744_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDU0556155.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37675..38988)
                     /locus_tag="RD744_000120"
     CDS             complement(37675..38988)
                     /locus_tag="RD744_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDU0556156.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39233..39457)
                     /locus_tag="RD744_000121"
     CDS             complement(39233..39457)
                     /locus_tag="RD744_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556157.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39477..39899)
                     /locus_tag="RD744_000122"
     CDS             complement(39477..39899)
                     /locus_tag="RD744_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDU0556158.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40043..40753)
                     /locus_tag="RD744_000123"
     CDS             complement(40043..40753)
                     /locus_tag="RD744_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556159.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40771..41268)
                     /locus_tag="RD744_000124"
     CDS             complement(40771..41268)
                     /locus_tag="RD744_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0556160.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41305..41757)
                     /locus_tag="RD744_000125"
     CDS             complement(41305..41757)
                     /locus_tag="RD744_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0556161.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41892..42497)
                     /locus_tag="RD744_000126"
     CDS             complement(41892..42497)
                     /locus_tag="RD744_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDU0556162.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42947..43567)
                     /locus_tag="RD744_000127"
     CDS             complement(42947..43567)
                     /locus_tag="RD744_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0556163.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43569..44438)
                     /locus_tag="RD744_000128"
     CDS             complement(43569..44438)
                     /locus_tag="RD744_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDU0556164.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44461..44868)
                     /locus_tag="RD744_000129"
     CDS             complement(44461..44868)
                     /locus_tag="RD744_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDU0556165.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44887..46176)
                     /locus_tag="RD744_000130"
     CDS             complement(44887..46176)
                     /locus_tag="RD744_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0556166.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46203..46505)
                     /locus_tag="RD744_000131"
     CDS             complement(46203..46505)
                     /locus_tag="RD744_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0556167.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46530..48623)
                     /locus_tag="RD744_000132"
     CDS             complement(46530..48623)
                     /locus_tag="RD744_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0556168.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49112..49876)
                     /locus_tag="RD744_000133"
     CDS             complement(49112..49876)
                     /locus_tag="RD744_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDU0556169.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49987..50856)
                     /gene="mdcB"
                     /locus_tag="RD744_000134"
     CDS             complement(49987..50856)
                     /gene="mdcB"
                     /locus_tag="RD744_000134"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDU0556170.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50881..51957)
                     /locus_tag="RD744_000135"
     CDS             complement(50881..51957)
                     /locus_tag="RD744_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDU0556171.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51981..52574)
                     /locus_tag="RD744_000136"
     CDS             complement(51981..52574)
                     /locus_tag="RD744_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDU0556172.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52578..54254)
                     /gene="mdcD"
                     /locus_tag="RD744_000137"
     CDS             complement(52578..54254)
                     /gene="mdcD"
                     /locus_tag="RD744_000137"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDU0556173.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54244..54543)
                     /locus_tag="RD744_000138"
     CDS             complement(54244..54543)
                     /locus_tag="RD744_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDU0556174.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54645..56291)
                     /gene="mdcA"
                     /locus_tag="RD744_000139"
     CDS             complement(54645..56291)
                     /gene="mdcA"
                     /locus_tag="RD744_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDU0556175.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56298..57209)
                     /locus_tag="RD744_000140"
     CDS             complement(56298..57209)
                     /locus_tag="RD744_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDU0556176.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57329..58186
                     /locus_tag="RD744_000141"
     CDS             57329..58186
                     /locus_tag="RD744_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0556177.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58373..58735)
                     /locus_tag="RD744_000142"
     CDS             complement(58373..58735)
                     /locus_tag="RD744_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDU0556178.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58924..59106)
                     /locus_tag="RD744_000143"
     CDS             complement(58924..59106)
                     /locus_tag="RD744_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556179.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59081..60520
                     /locus_tag="RD744_000144"
     CDS             59081..60520
                     /locus_tag="RD744_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556180.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60527..63079)
                     /locus_tag="RD744_000145"
     CDS             complement(60527..63079)
                     /locus_tag="RD744_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDU0556181.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63193..64524)
                     /locus_tag="RD744_000146"
     CDS             complement(63193..64524)
                     /locus_tag="RD744_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0556182.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64490..65968)
                     /locus_tag="RD744_000147"
     CDS             complement(64490..65968)
                     /locus_tag="RD744_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0556183.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66273..68108)
                     /gene="tdc"
                     /locus_tag="RD744_000148"
     CDS             complement(66273..68108)
                     /gene="tdc"
                     /locus_tag="RD744_000148"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDU0556184.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19443 a        14587 c        11702 g        22634 t
ORIGIN      
        1 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
       61 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      121 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      181 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      241 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      301 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      361 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      421 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      481 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      541 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      601 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa
      661 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      721 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      781 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      841 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      901 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
      961 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1021 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1081 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1141 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1201 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1261 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1321 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1381 tttttccaag cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1441 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1501 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1561 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1621 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1681 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1741 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1801 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1861 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1921 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc
     1981 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2041 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2101 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2161 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2221 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2281 ctattcggat ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt
     2341 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2401 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2461 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2521 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2581 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2641 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2701 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2761 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2821 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2881 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     2941 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3001 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3061 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3121 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3181 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3241 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3301 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3361 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3421 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3481 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3541 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3601 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3661 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3721 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3781 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3841 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3901 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     3961 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4021 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4081 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4141 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4201 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4261 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4321 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4381 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4441 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4501 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4561 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4621 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4681 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4741 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4801 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4861 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4921 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     4981 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5041 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5101 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5161 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5221 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5281 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5341 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5401 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5461 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5521 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5581 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5641 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5701 atccgcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5761 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5821 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5881 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     5941 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6001 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6061 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6121 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6181 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6241 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6301 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6361 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6421 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
     6481 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6541 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6601 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6661 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6721 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6781 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6841 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6901 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     6961 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7021 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7081 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7141 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7201 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7261 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7321 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7381 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7441 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7501 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7561 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7621 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7681 tttcagcacc agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt
     7741 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7801 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7861 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7921 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     7981 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8041 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8101 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8161 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8221 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8281 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8341 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat
     8401 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8461 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8521 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8581 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8641 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8701 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8761 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8821 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8881 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     8941 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc
     9001 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9061 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9121 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata
     9181 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
     9241 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
     9301 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
     9361 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
     9421 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
     9481 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
     9541 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
     9601 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
     9661 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
     9721 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
     9781 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
     9841 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
     9901 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
     9961 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    10021 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    10081 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    10141 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    10201 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    10261 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    10321 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    10381 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    10441 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    10501 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    10561 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    10621 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    10681 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    10741 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    10801 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    10861 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    10921 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    10981 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    11041 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    11101 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    11161 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    11221 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    11281 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    11341 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    11401 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    11461 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    11521 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    11581 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    11641 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    11701 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    11761 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    11821 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    11881 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    11941 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    12001 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    12061 ttttttaaag tggtggaccc gattagttta cctcttttaa gagtcgaatg caagcttttt
    12121 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    12181 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    12241 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    12301 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    12361 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    12421 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    12481 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    12541 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    12601 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    12661 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    12721 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    12781 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    12841 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    12901 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    12961 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    13021 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    13081 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    13141 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    13201 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    13261 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    13321 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    13381 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    13441 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    13501 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    13561 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    13621 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    13681 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    13741 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    13801 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    13861 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    13921 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    13981 tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa
    14041 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    14101 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    14161 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    14221 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    14281 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    14341 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    14401 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    14461 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    14521 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    14581 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    14641 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    14701 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    14761 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    14821 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    14881 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    14941 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    15001 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    15061 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    15121 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    15181 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    15241 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    15301 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    15361 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    15421 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    15481 caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg
    15541 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    15601 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    15661 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    15721 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    15781 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    15841 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    15901 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    15961 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    16021 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    16081 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    16141 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    16201 aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt
    16261 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    16321 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    16381 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    16441 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    16501 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    16561 caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg
    16621 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    16681 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    16741 cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat
    16801 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    16861 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    16921 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    16981 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    17041 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    17101 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    17161 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    17221 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    17281 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    17341 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    17401 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt
    17461 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    17521 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    17581 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    17641 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    17701 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    17761 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    17821 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    17881 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    17941 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    18001 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    18061 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    18121 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    18181 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    18241 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    18301 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    18361 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    18421 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    18481 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    18541 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    18601 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    18661 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    18721 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    18781 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    18841 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    18901 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    18961 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    19021 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    19081 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    19141 acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg
    19201 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    19261 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    19321 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    19381 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    19441 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    19501 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    19561 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    19621 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    19681 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    19741 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    19801 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    19861 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    19921 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    19981 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    20041 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    20101 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    20161 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    20221 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    20281 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    20341 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    20401 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    20461 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    20521 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    20581 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    20641 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    20701 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    20761 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    20821 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    20881 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    20941 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    21001 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    21061 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    21121 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    21181 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    21241 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    21301 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    21361 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    21421 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    21481 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    21541 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    21601 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    21661 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    21721 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    21781 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    21841 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    21901 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    21961 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    22021 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    22081 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    22141 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    22201 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    22261 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    22321 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    22381 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    22441 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    22501 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    22561 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    22621 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    22681 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    22741 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    22801 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    22861 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    22921 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    22981 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    23041 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    23101 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    23161 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    23221 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    23281 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    23341 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    23401 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    23461 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    23521 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    23581 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    23641 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    23701 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    23761 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    23821 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    23881 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    23941 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    24001 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    24061 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    24121 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    24181 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    24241 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    24301 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    24361 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    24421 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    24481 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    24541 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    24601 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    24661 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    24721 ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    24781 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    24841 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    24901 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    24961 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    25021 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    25081 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    25141 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    25201 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    25261 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    25321 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    25381 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    25441 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    25501 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    25561 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    25621 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    25681 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    25741 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    25801 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    25861 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    25921 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    25981 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    26041 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    26101 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    26161 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    26221 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    26281 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    26341 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    26401 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    26461 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    26521 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    26581 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    26641 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    26701 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    26761 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    26821 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    26881 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    26941 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    27001 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    27061 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    27121 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    27181 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    27241 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    27301 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    27361 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    27421 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    27481 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    27541 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    27601 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    27661 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    27721 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    27781 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    27841 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    27901 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    27961 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    28021 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    28081 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    28141 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    28201 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    28261 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    28321 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    28381 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    28441 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    28501 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    28561 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    28621 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    28681 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    28741 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    28801 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    28861 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat
    28921 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    28981 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    29041 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    29101 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    29161 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    29221 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    29281 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    29341 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    29401 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    29461 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    29521 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    29581 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    29641 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    29701 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    29761 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    29821 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    29881 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    29941 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    30001 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    30061 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    30121 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    30181 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    30241 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    30301 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    30361 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    30421 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc
    30481 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    30541 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    30601 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    30661 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    30721 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    30781 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    30841 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    30901 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    30961 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    31021 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    31081 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    31141 cagctgactt gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac
    31201 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    31261 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    31321 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    31381 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    31441 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    31501 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    31561 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    31621 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    31681 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    31741 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    31801 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    31861 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    31921 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    31981 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    32041 accccttata atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    32101 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    32161 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    32221 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    32281 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    32341 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    32401 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    32461 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    32521 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    32581 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    32641 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    32701 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    32761 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    32821 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    32881 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    32941 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    33001 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    33061 ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat
    33121 aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac
    33181 gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag
    33241 aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg
    33301 ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct
    33361 ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa
    33421 tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt
    33481 tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa
    33541 gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac
    33601 ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg
    33661 tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt
    33721 ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg
    33781 attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt
    33841 acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc
    33901 agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga
    33961 taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat
    34021 cattttgtct atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg
    34081 atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg
    34141 cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc
    34201 gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta
    34261 attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc
    34321 aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc
    34381 cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg
    34441 caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg
    34501 cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga
    34561 catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat
    34621 cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat
    34681 ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac
    34741 aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag
    34801 ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa
    34861 aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt
    34921 caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg
    34981 gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga
    35041 ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa
    35101 atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac
    35161 gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg
    35221 attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct
    35281 ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg
    35341 caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt
    35401 cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat
    35461 tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac
    35521 tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa agaaaacact
    35581 cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt
    35641 aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa
    35701 gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat
    35761 tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat
    35821 tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac
    35881 atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt
    35941 ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat
    36001 tgcataggcg tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct
    36061 tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct
    36121 tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa
    36181 ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac
    36241 acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa
    36301 ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca
    36361 gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg
    36421 tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg
    36481 atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt
    36541 gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta
    36601 tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg
    36661 atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa
    36721 tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc
    36781 caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca
    36841 cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac
    36901 ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc
    36961 tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct
    37021 tgataattac tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc
    37081 caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa
    37141 tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact
    37201 gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt
    37261 tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt
    37321 gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg
    37381 ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac
    37441 ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37501 tttttcgttt ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa
    37561 cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta
    37621 ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt
    37681 cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga
    37741 tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc
    37801 aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat
    37861 cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac
    37921 cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa
    37981 caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac
    38041 ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc
    38101 cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc
    38161 tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt
    38221 cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg
    38281 ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg
    38341 cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat
    38401 gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa
    38461 ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat
    38521 gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc
    38581 atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat
    38641 caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc
    38701 cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc
    38761 aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt
    38821 cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa
    38881 taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt
    38941 ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc
    39001 taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg
    39061 ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc
    39121 cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt
    39181 acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt
    39241 gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag
    39301 gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct
    39361 tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat
    39421 acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa
    39481 tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa
    39541 gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt
    39601 tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac
    39661 ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca
    39721 atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct
    39781 acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt
    39841 atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt
    39901 atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt
    39961 ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc
    40021 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    40081 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    40141 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    40201 tttcttccgt cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    40261 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    40321 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    40381 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    40441 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    40501 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    40561 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    40621 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    40681 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    40741 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    40801 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    40861 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    40921 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    40981 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    41041 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    41101 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    41161 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    41221 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    41281 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    41341 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    41401 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    41461 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    41521 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    41581 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    41641 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    41701 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    41761 atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat
    41821 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    41881 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    41941 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    42001 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    42061 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    42121 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    42181 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    42241 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    42301 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    42361 cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    42421 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    42481 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    42541 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    42601 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    42661 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    42721 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    42781 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    42841 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    42901 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    42961 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    43021 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    43081 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    43141 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    43201 accgacgttg acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    43261 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    43321 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    43381 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    43441 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    43501 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    43561 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    43621 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    43681 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    43741 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    43801 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    43861 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    43921 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    43981 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    44041 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    44101 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    44161 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    44221 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    44281 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    44341 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    44401 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    44461 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    44521 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    44581 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    44641 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    44701 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    44761 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    44821 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    44881 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    44941 tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac
    45001 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    45061 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    45121 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    45181 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    45241 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    45301 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    45361 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    45421 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    45481 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    45541 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    45601 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    45661 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac
    45721 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    45781 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    45841 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    45901 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    45961 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    46021 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    46081 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    46141 caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt
    46201 atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt
    46261 gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac
    46321 tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa
    46381 atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac
    46441 atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg
    46501 ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt
    46561 ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc
    46621 aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa
    46681 aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta
    46741 gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg
    46801 tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca
    46861 tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc
    46921 tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac
    46981 ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac
    47041 ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga
    47101 gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt
    47161 ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa
    47221 agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta
    47281 tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt
    47341 tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact
    47401 gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg
    47461 acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga
    47521 acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat
    47581 ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag
    47641 agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca
    47701 aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga attggagata
    47761 tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca
    47821 ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt
    47881 atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc
    47941 cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac
    48001 ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag
    48061 attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga
    48121 ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc
    48181 agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac
    48241 cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt
    48301 agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc
    48361 cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc
    48421 ttttccagct tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta
    48481 ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg
    48541 aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt
    48601 atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc
    48661 gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc
    48721 aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta
    48781 ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa
    48841 gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta
    48901 tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc
    48961 ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta
    49021 ttgccgatag gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt
    49081 ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat
    49141 cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact
    49201 gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac
    49261 agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta
    49321 cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca
    49381 gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag
    49441 tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg
    49501 ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct
    49561 tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta
    49621 atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat
    49681 aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa
    49741 atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag
    49801 cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat
    49861 aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat
    49921 agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg
    49981 tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc
    50041 tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg
    50101 gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact
    50161 agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac
    50221 gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata
    50281 tgaatcatta tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa
    50341 tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt
    50401 ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag
    50461 tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa
    50521 tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat
    50581 tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc
    50641 atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt
    50701 aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc
    50761 aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg
    50821 ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta
    50881 ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc
    50941 aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg
    51001 cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata
    51061 cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac
    51121 aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg
    51181 gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga
    51241 acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc
    51301 caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact
    51361 agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt
    51421 ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac
    51481 atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg
    51541 gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt
    51601 cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca
    51661 aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa
    51721 aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat
    51781 aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt
    51841 tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac
    51901 agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc
    51961 tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt
    52021 ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc
    52081 cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga
    52141 attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt
    52201 actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat
    52261 cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc
    52321 tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca
    52381 tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt
    52441 agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga
    52501 acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca
    52561 tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt
    52621 gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg
    52681 tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca
    52741 ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt
    52801 tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt
    52861 tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta
    52921 atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca
    52981 cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt
    53041 gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag
    53101 ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct
    53161 ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca
    53221 acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg
    53281 atggcttctt ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt
    53341 ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt
    53401 tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct
    53461 tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa
    53521 taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca
    53581 gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc
    53641 cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga
    53701 acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt
    53761 gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt
    53821 cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt
    53881 tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt
    53941 acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca
    54001 gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata
    54061 gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca
    54121 ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca
    54181 atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca
    54241 aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc
    54301 tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa
    54361 tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac
    54421 agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac
    54481 tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc
    54541 catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt
    54601 cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg
    54661 aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt
    54721 gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc
    54781 acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt
    54841 gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac
    54901 aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact
    54961 ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag
    55021 actggttttt tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc
    55081 cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg
    55141 ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg
    55201 gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca
    55261 tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca
    55321 aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta
    55381 tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat
    55441 acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac
    55501 aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct
    55561 gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga
    55621 ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct
    55681 acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg
    55741 atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg
    55801 aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt
    55861 ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat
    55921 ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag
    55981 aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata
    56041 ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct
    56101 gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca
    56161 atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag
    56221 ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt
    56281 ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca
    56341 tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc
    56401 tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc
    56461 aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga
    56521 atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca
    56581 tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag
    56641 agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg
    56701 gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga
    56761 caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc
    56821 tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct tggataagct
    56881 tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat
    56941 cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga
    57001 ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg
    57061 ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt
    57121 tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct
    57181 tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc
    57241 actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa
    57301 caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa
    57361 agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc
    57421 cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag
    57481 cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac
    57541 attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac
    57601 ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt
    57661 tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat
    57721 taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat
    57781 catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca
    57841 tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt
    57901 ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac
    57961 gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt
    58021 cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga
    58081 tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat
    58141 caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg
    58201 ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt
    58261 agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat
    58321 ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg
    58381 ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta
    58441 gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
    58501 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat
    58561 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
    58621 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
    58681 gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct
    58741 tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga
    58801 atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt
    58861 tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc
    58921 cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc
    58981 agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt
    59041 tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa
    59101 aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca
    59161 tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt
    59221 ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca
    59281 agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt
    59341 tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc
    59401 tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac
    59461 taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc
    59521 ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt
    59581 cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt
    59641 tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg
    59701 ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca
    59761 taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga
    59821 aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg
    59881 gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt
    59941 agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa
    60001 aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat
    60061 attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata
    60121 tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat
    60181 caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc
    60241 tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc
    60301 gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga
    60361 actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc
    60421 tctagattct ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct
    60481 caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt
    60541 ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta
    60601 caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac
    60661 ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag
    60721 caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg
    60781 gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca
    60841 gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa
    60901 ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt
    60961 gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg
    61021 cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag
    61081 gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg
    61141 gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg
    61201 cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta
    61261 cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat
    61321 catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa
    61381 ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa
    61441 tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt
    61501 ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt
    61561 tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg
    61621 gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt
    61681 cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa
    61741 attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa
    61801 ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg
    61861 ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta
    61921 gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt
    61981 ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc
    62041 aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg
    62101 tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga
    62161 tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag
    62221 ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat
    62281 gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa
    62341 tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt
    62401 gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta
    62461 caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct
    62521 ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt
    62581 ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg
    62641 ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag
    62701 ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca
    62761 aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga
    62821 tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag
    62881 aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc
    62941 gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc
    63001 gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg
    63061 tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag
    63121 ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa
    63181 atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa
    63241 tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct
    63301 gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt
    63361 ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag
    63421 aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa
    63481 aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg
    63541 tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg
    63601 atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa
    63661 aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac
    63721 taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat
    63781 cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt
    63841 cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact
    63901 gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc
    63961 gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa
    64021 tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga
    64081 ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc
    64141 gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat
    64201 tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa
    64261 aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact
    64321 atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat
    64381 caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa
    64441 taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt
    64501 ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga
    64561 cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa
    64621 tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc
    64681 cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc
    64741 tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca
    64801 atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat
    64861 ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga
    64921 ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct
    64981 tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg
    65041 ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa
    65101 acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg
    65161 cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca
    65221 aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa
    65281 ctggattttc caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca
    65341 tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc
    65401 atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc
    65461 ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc
    65521 cgatttttgc catatcaaat ttcaaattca aaagagtgat gatccaataa acgactaaaa
    65581 tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa
    65641 acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca
    65701 gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac
    65761 cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt
    65821 acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg
    65881 gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga
    65941 tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt
    66001 tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca
    66061 aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga
    66121 aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat
    66181 gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg
    66241 tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt
    66301 tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa
    66361 ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc
    66421 gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat
    66481 gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga
    66541 tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg
    66601 aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt
    66661 cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt
    66721 gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc
    66781 cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata
    66841 tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc
    66901 atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga
    66961 ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg
    67021 tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca
    67081 tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg
    67141 gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct taattggacg
    67201 attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct
    67261 agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc
    67321 attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata
    67381 tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt
    67441 tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta
    67501 aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac
    67561 atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa
    67621 cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat
    67681 ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa
    67741 gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa
    67801 gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa
    67861 tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata
    67921 tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata
    67981 tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac
    68041 attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata
    68101 tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta
    68161 tctgtaacac ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat
    68221 ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca
    68281 aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta aaaaaagcga
    68341 acaaacaaat aaaatgactg tccttc
//
LOCUS       DAPCMU010000003        48221 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPCMU010000003 DAPCMU010000000
VERSION     DAPCMU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 48221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.003"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     regulatory      72..169
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            285..866
                     /locus_tag="RD744_000149"
     CDS             285..866
                     /locus_tag="RD744_000149"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDU0556185.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            869..2176
                     /locus_tag="RD744_000150"
     CDS             869..2176
                     /locus_tag="RD744_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDU0556186.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2469..2957
                     /gene="purE"
                     /locus_tag="RD744_000151"
     CDS             2469..2957
                     /gene="purE"
                     /locus_tag="RD744_000151"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDU0556187.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2950..4074
                     /gene="purK"
                     /locus_tag="RD744_000152"
     CDS             2950..4074
                     /gene="purK"
                     /locus_tag="RD744_000152"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDU0556188.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4087..5382
                     /gene="purB"
                     /locus_tag="RD744_000153"
     CDS             4087..5382
                     /gene="purB"
                     /locus_tag="RD744_000153"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDU0556189.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(5562..5939)
                     /locus_tag="RD744_000154"
     CDS             complement(5562..5939)
                     /locus_tag="RD744_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556190.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            6195..6923
                     /locus_tag="RD744_000155"
     CDS             6195..6923
                     /locus_tag="RD744_000155"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDU0556191.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            6923..7177
                     /gene="purS"
                     /locus_tag="RD744_000156"
     CDS             6923..7177
                     /gene="purS"
                     /locus_tag="RD744_000156"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDU0556192.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            7179..7850
                     /gene="purQ"
                     /locus_tag="RD744_000157"
     CDS             7179..7850
                     /gene="purQ"
                     /locus_tag="RD744_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDU0556193.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            7851..10073
                     /gene="purL"
                     /locus_tag="RD744_000158"
     CDS             7851..10073
                     /gene="purL"
                     /locus_tag="RD744_000158"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDU0556194.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            10058..11497
                     /locus_tag="RD744_000159"
     CDS             10058..11497
                     /locus_tag="RD744_000159"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDU0556195.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            11520..12572
                     /locus_tag="RD744_000160"
     CDS             11520..12572
                     /locus_tag="RD744_000160"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDU0556196.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            12569..13147
                     /gene="purN"
                     /locus_tag="RD744_000161"
     CDS             12569..13147
                     /gene="purN"
                     /locus_tag="RD744_000161"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDU0556197.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            13144..14679
                     /gene="purH"
                     /locus_tag="RD744_000162"
     CDS             13144..14679
                     /gene="purH"
                     /locus_tag="RD744_000162"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDU0556198.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            14695..15969
                     /gene="purD"
                     /locus_tag="RD744_000163"
     CDS             14695..15969
                     /gene="purD"
                     /locus_tag="RD744_000163"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDU0556199.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            16091..17005
                     /locus_tag="RD744_000164"
     CDS             16091..17005
                     /locus_tag="RD744_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0556200.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(17138..18703)
                     /locus_tag="RD744_000165"
     CDS             complement(17138..18703)
                     /locus_tag="RD744_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0556201.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(18696..20147)
                     /locus_tag="RD744_000166"
     CDS             complement(18696..20147)
                     /locus_tag="RD744_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0556202.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(20161..21177)
                     /locus_tag="RD744_000167"
     CDS             complement(20161..21177)
                     /locus_tag="RD744_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0556203.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            21397..22485
                     /locus_tag="RD744_000168"
     CDS             21397..22485
                     /locus_tag="RD744_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDU0556204.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            22539..24065
                     /locus_tag="RD744_000169"
     CDS             22539..24065
                     /locus_tag="RD744_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDU0556205.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            24067..25272
                     /locus_tag="RD744_000170"
     CDS             24067..25272
                     /locus_tag="RD744_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0556206.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            25529..26506
                     /gene="guaC"
                     /locus_tag="RD744_000171"
     CDS             25529..26506
                     /gene="guaC"
                     /locus_tag="RD744_000171"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDU0556207.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            26770..28194
                     /locus_tag="RD744_000172"
     CDS             26770..28194
                     /locus_tag="RD744_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDU0556208.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            28655..29764
                     /gene="pdhA"
                     /locus_tag="RD744_000173"
     CDS             28655..29764
                     /gene="pdhA"
                     /locus_tag="RD744_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDU0556209.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            29767..30744
                     /locus_tag="RD744_000174"
     CDS             29767..30744
                     /locus_tag="RD744_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDU0556210.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRKLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            30769..32412
                     /locus_tag="RD744_000175"
     CDS             30769..32412
                     /locus_tag="RD744_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDU0556211.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            32420..33826
                     /gene="lpdA"
                     /locus_tag="RD744_000176"
     CDS             32420..33826
                     /gene="lpdA"
                     /locus_tag="RD744_000176"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDU0556212.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            33988..34263
                     /locus_tag="RD744_000177"
     CDS             33988..34263
                     /locus_tag="RD744_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDU0556213.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            34269..35036
                     /locus_tag="RD744_000178"
     CDS             34269..35036
                     /locus_tag="RD744_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDU0556214.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            35204..37033
                     /gene="typA"
                     /locus_tag="RD744_000179"
     CDS             35204..37033
                     /gene="typA"
                     /locus_tag="RD744_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDU0556215.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            37294..39066
                     /gene="recQ"
                     /locus_tag="RD744_000180"
     CDS             37294..39066
                     /gene="recQ"
                     /locus_tag="RD744_000180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDU0556216.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            39180..39482
                     /locus_tag="RD744_000181"
     CDS             39180..39482
                     /locus_tag="RD744_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDU0556217.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            39485..40648
                     /locus_tag="RD744_000182"
     CDS             39485..40648
                     /locus_tag="RD744_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDU0556218.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            40674..44102
                     /locus_tag="RD744_000183"
     CDS             40674..44102
                     /locus_tag="RD744_000183"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDU0556219.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            44123..45247
                     /locus_tag="RD744_000184"
     CDS             44123..45247
                     /locus_tag="RD744_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556220.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            45237..45674
                     /locus_tag="RD744_000185"
     CDS             45237..45674
                     /locus_tag="RD744_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDU0556221.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            45658..45996
                     /locus_tag="RD744_000186"
     CDS             45658..45996
                     /locus_tag="RD744_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDU0556222.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            46115..46675
                     /gene="rsmD"
                     /locus_tag="RD744_000187"
     CDS             46115..46675
                     /gene="rsmD"
                     /locus_tag="RD744_000187"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDU0556223.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            46672..47163
                     /gene="coaD"
                     /locus_tag="RD744_000188"
     CDS             46672..47163
                     /gene="coaD"
                     /locus_tag="RD744_000188"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDU0556224.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            47153..48196
                     /locus_tag="RD744_000189"
     CDS             47153..48196
                     /locus_tag="RD744_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDU0556225.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        15813 a         8263 c        10818 g        13327 t
ORIGIN      
        1 acatttttta ttcaaatatg gcaaactgcc ttcacgtgca aaacagttgc aagaaaatga
       61 atttacacta ttattagttt tacataccta cttatatatc gtcataatat ggatgacagt
      121 ttctagccag taccgtaaat gctggactat aagtaaaaga ttggctattt cacccttctt
      181 ttcttatgat aaagtggagg gtgtttttct ttaacagaaa catagtaggt ttttgcacaa
      241 aagcggttat ttcggtatta ataaatgata aaggagcaaa taaagtgaaa gaattagttg
      301 aacgtattca aaaagacgga cgtatattag gcgagggcgt attaaaagtc gacagcttcg
      361 tcactcacca agtagaccct gtattgatgg aacaaatggg aaaacggttt gcagaagtat
      421 ttaaagaaca aggaatcacg aaagttgtga ccattgaagc ttctgggatc gcaccagctt
      481 tatttgctgc aagagaatta gatgttccaa tgatcttcgc tagaaaagca aaaagtttga
      541 cgatggatga agaattattg acagcttctg tttattcttt tacgaaacaa gtgacaagta
      601 cgatttctat ttctcgtaaa tttttatctt cagaagacaa agtattgatt atcgacgatt
      661 tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt atgccagcaa gctggagcgc
      721 aagtagaagg aatcgggatc gtcatcgaaa agtctttcca agatggacgt cagttacttg
      781 aagaaatggg attgagagtc gtttctcttg cgcgtattgc ttccttgacg aatggacaag
      841 ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa acacaaaatg gaaaagctgc
      901 ggttttaggg ctgcagcatc tgctagcgat gtatgcagga gcagtagcgg tgcctctttt
      961 gatcggtaca ggactaggct ttaatcaaga acagatgact tatttgattt caattgatat
     1021 ctttatgtgt ggattagcga cattattgca attgacagtt aaccgatttt tcggaattgg
     1081 cttgcctgtc gttttaggat gtgcgatcca agcagtagct ccactgatct tgatcggaac
     1141 caatgaagga gtaggagcta tctacggatc tattatcgct tctgggatct ttgttgtgtt
     1201 agtttctggt gtcttttcta aaattaaaaa actattccct cctatagtaa caggaacggt
     1261 tatcaccgtt attggtttga ctttgattcc tgtagcaatc gagaaaatgg gtggaggtaa
     1321 tgcaacagca gctgattttg gtgacaaaac aaatctgcta cttgcatttg tgacgattct
     1381 cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt cgttcaatcg cggtattgat
     1441 tggtttagtc ggaggaagca tcttagctgc tttcatggga atgattgatc tgagcgcgat
     1501 tggagatgcg ccgttgttcc atgttccaca acccttttat tttggtaaac caactttcga
     1561 cgtatggtcg attttactaa tgatcatcat ttccatggtc agcatggtag aatcaacagg
     1621 tgtatatttt gcacttggtg acattactgg gaaaaatgtt ggagaagaag aattgaaaaa
     1681 gggatatcgt gcagaaggac ttgccgttat cttaggcggt attttcaata cattccctta
     1741 tactggcttt tctcaaaatg tcggattggt tcaactttcg ggaatcaaaa caagaaaacc
     1801 catctatttc tctgcattct tcttgattgt tttaggatta ttccctaaaa tcggtgcgtt
     1861 agcacaaatc atccctgagc cggtacttgg tggaggaatg ttagtcatgt tcgggatggt
     1921 ggcagtccaa ggaatccgta tgctgttgga agttgatttc agcaatgata aaaatctatt
     1981 gattgttgct gtttctgttg gatttgggct gggtttcaat gttacgccga cattattcag
     2041 tcaaatgccg caaacattgc aaatgttcac tggaaatggc atcgtaatga gtagcattac
     2101 ggctatcata ttgaatttga tctttaatgg attgaaaaaa gatgaagaaa tattggaaga
     2161 agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa tcagcgtttt gacctgattg
     2221 ttagagaaac aaacggaaaa atagttcctc gtattttgcc gtttgtttga gctaataccg
     2281 agggtcagct gattgaacac cgtttatcca taaaaaggaa tgtgacctaa cttttgtcac
     2341 attccttttt ttcatcttta ataaacacga acaataaaat gtttttcttt gttaacattc
     2401 gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat gaatgtagaa aaactgggag
     2461 gaaaaacaat gacagcaaaa atctctgtga ttatggggag tacttcagat tgggaaacaa
     2521 tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc ttatgaaaaa aaggtcgttt
     2581 ctgctcatcg aaccccagat ttgatgtttg aatatgcaca aaacgctcga aaaaaaggaa
     2641 tcaaaatcat tattgcagga gcagggggag ccgcgcatct gccgggtatg gtcgcatcaa
     2701 aaacaacttt gccggttatc ggtgtaccgg tccaatcacg aacattaaat ggtttggatt
     2761 cgcttctttc gattgtccaa atgcctggag gagtacctgt agctactaca gcaatcggaa
     2821 aagcgggagc aattaatgct ggactacttg cgatacagat gctttccatg tatgatttag
     2881 aattggaggc taaattggct gaaagacgtt caatgttggc taaaactgtt atagaaagta
     2941 gtgatcaact tggttaaacc tttgttgcct ggttcaacga tcgggatcgt cggtggcggt
     3001 caattaggtc gtatgatggc gattagtgca aaagaaatgg gctttaaagt aggtgtgctg
     3061 gatcctgtga gcgattgtcc agccgcacaa gttgcagatt ggcacattga aagtgtctat
     3121 gatgacacct ttgcgctgaa agagcttgcc agaagaacag atgtcattac ctatgaattc
     3181 gaaaacgtca gtgtagaggc gttaaatgcg attttaccca tgtcatttat tcctcaaggg
     3241 acagacttgc ttgcgattac gcaagaccgc ctgttagaaa aatctttttt ggaaacgaat
     3301 aacatcgtga ttgctcctta tgcaacgatc gtcagtccga cggatatcca agatgcgatt
     3361 gacgggatcg gctatccttg tgttttgaaa accactcgag gcggctatga tggcaagggg
     3421 cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca tggacctttt aagagaagga
     3481 acctgtgttt tagaagcatg gatcccgttt gaaaaagaga tttcgatcat ggtggcagga
     3541 aatggtcaag gagatttcac gacttttccg gtcgtggaaa acatccacca caacaatatt
     3601 cttcatgaaa cgattgctcc agccgcgatt gatcaggatg tgatcgagga ggctgagcgt
     3661 attgcccgtg tgattgcaga agcagtgagc ctttcaggtg ttttaggggt agaaatgttt
     3721 ttaacaaaaa caggcgggct atatgtgaat gaattagctc ctcgtcctca caattctgga
     3781 cattattcta ttgaagcttg ttctatgagt cagttcgatg cccatattcg aggtatttgt
     3841 ggttggccat taggagaaaa agtacgtttg ataagcaatg cagtaatggt caatgttttg
     3901 ggagaacaac tgctggaaag ctatcgcttg attgagaata agccagactg gcaattccat
     3961 ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg gacatatcac tatcttgaca
     4021 gaagacatcc agcaaacgct ggaagaaatc aaacaaacga atatttggga ttagaggaga
     4081 aaaaacatgt tagaacgtta tacacgccct gaaatgggga atatctggac ggaagagaac
     4141 aaataccgtg cgtggctcga agtcgagatt ttagcagatg aagcttgggc agaacttggt
     4201 gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg catcatttga tatcgatcgg
     4261 atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg cgtttacacg agcggtttct
     4321 gaaacattgg gagaagaaag aaaatgggtc cattatggtc taacaagtac ggatgtcgta
     4381 gatactgctt atggctatct catcaagcaa gcaaatgata ttttacggga agacttgaaa
     4441 cgttttacgg aaatcattgg cgaaaaagca aaagaacaca aatatacagt gatgatgggt
     4501 cggacacacg gtgtccatgc tgaaccaact acttttggat tgaaacttgc cacatggtat
     4561 tcagaaatga aacggaacat cgaacgattt gaacatgcgg caaaaggagt agaagcagga
     4621 aaaatctcag gagcagtggg aacgttcgca aatatcccgc catttattga agagtatgta
     4681 tgtaaacaat tggggatccg tccacaagaa atctctactc aagttctccc tcgggatcta
     4741 catgctgaat atttctcagc gatggcgctt attgctacaa gtattgaacg ttttgcaaca
     4801 gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag aagaattttt tgcgaaaggt
     4861 caaaaaggtt cttctgctat gccgcacaaa aggaatccga tcggttcaga aaatatggct
     4921 ggtctagcca gagtggtgcg cggacacatg gtcactgcct atgaaaatgt cacattatgg
     4981 catgaaagag atatttctca ttcttcagca gaacgaatca tcttgcctga taccacgatt
     5041 ttattgaatt acatgttaaa tcgttttggc aatatcgtta aaaatctaac ggtctttcct
     5101 gaaaacatga agcggaatat gtctgcaaca tttggtttga tttatagcca gcgcgttatg
     5161 ctgaaattga tcgataaggg aatgacaaga gaagaagctt atgatcttgt tcagccaaaa
     5221 actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc tgttagaaga agatccaacc
     5281 atcacatcgg tattatctaa agaagaaata gacgatgcct ttgattatca tcatcatttg
     5341 aaaaacgttg acgtgatatt caaacgagta ggtttggaat aaagaagagt gagttgcaaa
     5401 attcaattaa aatagaatag caaaaataac caaaaaagaa gcagatccgt tagcggttct
     5461 gcttctttta gtttattata ttttcttatc aataatagga acctgatttt aaaagcggct
     5521 acctctttac cgtgcctctt tacatacaaa aaatagagga attaagcgta aatggcttgg
     5581 ctattataac taaaatgctg gttatggaga tgttgcagca agttttcgag aaaagcgtat
     5641 tccatctctt gtccattgaa taagatctgg atacgataat gatttttgaa acgcttctgt
     5701 tcatgaatct gactgattga aagttcgcct ttgtgttctg tctgataaag aaaagtcctt
     5761 ttacggcatt cagcacagcg cttggttgct cggcggattt ttcggaatgt ataaaaacta
     5821 gtatctttat tgcataaata aacgtctttg atcgacagca aatcattata tagacaggaa
     5881 cgttcttcag ctgttaattc tgataaaaaa agctgacctt gttttattgc atcttccatc
     5941 ggcaagtgcc tcctcgaacc cattatccaa atttttagtt aattctaata taccatcatt
     6001 aagtataaat gtaaacgctt gttgatggta taagattttc gtttctgata aaacacgaac
     6061 attaaaagaa tctaatgcct taaatgtaca tatttttgtt gatttataaa aagatgattg
     6121 ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa
     6181 aaggagagac gtacatggag aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat
     6241 atcttacttc tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga
     6301 atggcgcgag gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg
     6361 caattttccg ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta
     6421 aaaccgaaca attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata
     6481 ttgcagcagg aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac
     6541 caatcattga attctactat aaggatgacc agttggatga tccatttatc aatgaagagc
     6601 atatctattt cttacaactt gcaaaaccgg aagaaatcag tgagctgaaa gcccaagcgt
     6661 tggctattaa tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt
     6721 tcaagctaga atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg
     6781 atacttgccg attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca
     6841 gagatctagg tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa
     6901 ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc
     6961 agtgttagat cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac
     7021 aatcgaagat atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc
     7081 aatcgataca gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac
     7141 ataccgatat gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt
     7201 ttccaggttc aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg
     7261 atgccgaatt tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc
     7321 cgggaggatt ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa
     7381 tcatggaaga agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg
     7441 gattccagat attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt
     7501 tgcattttat ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag
     7561 agtatgaagc cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct
     7621 gtgacgaaga aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg
     7681 acaacatcaa cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt
     7741 taggaatgat gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga
     7801 aacggttctt tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat
     7861 tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga
     7921 ctgatgaaga atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg
     7981 aaactggact gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg
     8041 ttctacgcaa atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag
     8101 gcatcgtcga catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc
     8161 cttcagccgt ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata
     8221 ttttcagcat gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag
     8281 ataatccaag aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca
     8341 attgtatcgg cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa
     8401 acccattagt caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc
     8461 aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta
     8521 tccacggagc aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg
     8581 ctgtacaagt aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa
     8641 tcttgcatca ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt
     8701 catctagtgc ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag
     8761 tcccacaaag agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac
     8821 ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt
     8881 atgatctaga tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc
     8941 atcaaggaga agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt
     9001 atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc
     9061 agccagttat cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg
     9121 cttcaaaacg ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc
     9181 gtccaggaag cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga
     9241 ccacagactg caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag
     9301 tggcagaagc tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact
     9361 gcctgaacta tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg
     9421 acggaattgc tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt
     9481 tatataatga aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat
     9541 tgattgagga tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct
     9601 atatattagg taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg
     9661 gaagaatcga aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt
     9721 tagtattgac agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg
     9781 gcttggcaat tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt
     9841 ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg
     9901 caccggaaaa taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta
     9961 aggtgacgga tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa
    10021 ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt
    10081 aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata
    10141 cttcgggctc catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga
    10201 tcgaggaaag cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca
    10261 aagaaaattg cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc
    10321 aggcaatgga agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat
    10381 cggcttagcg cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga
    10441 aaatggggcg atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg
    10501 aagtgaagaa acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg
    10561 ctttgcctat ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt
    10621 tcgtccatta gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg
    10681 tgcattagaa gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat
    10741 catcaatgac gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg
    10801 ttcgatggaa tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca
    10861 tactgcacgg aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat
    10921 ggtcgttggc gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg
    10981 aatcccttat gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc
    11041 aacgcctgag ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt
    11101 ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg
    11161 gatcgtccat cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc
    11221 tcctttgaaa tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc
    11281 ggcaaatcac acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt
    11341 aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt
    11401 gtgtatggct tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata
    11461 tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata
    11521 tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag
    11581 tagtagaacg gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta
    11641 taggaggatt cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg
    11701 tctcggggac cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg
    11761 atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag
    11821 agccgttata tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa
    11881 atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag
    11941 aaacagctga aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg
    12001 tagggatagc tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt
    12061 tgcttggaat agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt
    12121 tctttgagca gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg
    12181 gcgaagaatt gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag
    12241 aaaagaaaat aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc
    12301 gaatgttacc agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa
    12361 tattcggctg tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca
    12421 atatgggcat cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg
    12481 aaattgagca agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac
    12541 atcgtgtggt gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg
    12601 aagcaacttt caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga
    12661 ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt
    12721 accggcagat tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat
    12781 tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat
    12841 cattggaccg gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct
    12901 tttacctgcc ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga
    12961 aacgggtgtc acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca
    13021 ggaaaaagtg agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag
    13081 agtagagcat cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct
    13141 gaaatgacaa gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa
    13201 ggtttgattg aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat
    13261 aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat
    13321 ggccgtgtca aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc
    13381 cctagccata tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc
    13441 aatttatatc ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt
    13501 gaaaacattg atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt
    13561 gtcacctctg ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat
    13621 gggcatactt cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg
    13681 gcatatgatg cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa
    13741 aaattgacac ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa
    13801 gccgctgcct tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa
    13861 ctccatggga aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc
    13921 atgaaggaat atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt
    13981 gggacagcat ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca
    14041 atctttggtg ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg
    14101 cataagctgt ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta
    14161 ctgcaaaaaa agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa
    14221 aatcgagaag aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca
    14281 atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca
    14341 aaagcattag cgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg
    14401 gcaaacgaac gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta
    14461 aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct
    14521 tattttccaa tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt
    14581 cagccaggag gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta
    14641 gcaatggtct ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac
    14701 attttggtga tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt
    14761 ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc
    14821 aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag
    14881 gctattgatt ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat
    14941 tttatggcgg aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt
    15001 tcgaaaagtt ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga
    15061 agttttgaat gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta
    15121 gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa
    15181 gcgctggatg cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa
    15241 atcgtcgtag aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat
    15301 accaaggtgt atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa
    15361 gggccaaata caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg
    15421 attgaaaaag ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga
    15481 cgctctttca cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg
    15541 atcgaattca atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag
    15601 agtgactttg cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg
    15661 cagcaagaag gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat
    15721 gagacaggaa tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga
    15781 gtttcctcaa ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca
    15841 tacggtgaga cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg
    15901 acagatggaa cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc
    15961 gtgaagtaaa tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg
    16021 attttagtga agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg
    16081 aggagttcag atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat
    16141 cttaggctgt atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta
    16201 tgaaaacggc attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga
    16261 aatctttgca gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac
    16321 aaaatgtgga atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc
    16381 tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg
    16441 tccagataca ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc
    16501 aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa
    16561 aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg
    16621 aatgatcgat caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg
    16681 tagtgtactt gattattccc gattgcatga tatgacgatc caagcatggt caccatacca
    16741 gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac
    16801 attagaagaa ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat
    16861 tttacgccat ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa
    16921 ggaaatcgct caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg
    16981 tgctgctgga aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt
    17041 aaaaagacag aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt
    17101 gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga
    17161 aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga
    17221 aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt
    17281 gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt
    17341 ggttaaacga ttgagaaaaa atcgtactga aatgagaggg acttaatgca acttgttgag
    17401 cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat
    17461 ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag
    17521 aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt
    17581 gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat
    17641 gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt
    17701 gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat
    17761 caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat
    17821 cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga
    17881 tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa
    17941 aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg
    18001 tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa
    18061 cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat
    18121 gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt
    18181 tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg
    18241 agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag
    18301 aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt
    18361 tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc
    18421 ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta
    18481 gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca
    18541 gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt
    18601 cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat
    18661 tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt
    18721 gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca
    18781 atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa
    18841 agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct
    18901 gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca
    18961 tcaattttgc atgcggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg
    19021 tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt
    19081 gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact
    19141 agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt
    19201 aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag
    19261 tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc
    19321 tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc
    19381 cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat
    19441 tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac
    19501 atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt
    19561 ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc
    19621 gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct
    19681 tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg
    19741 gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt
    19801 gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta
    19861 tttttgatct ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca
    19921 tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc
    19981 ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc
    20041 tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct
    20101 ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag
    20161 tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat
    20221 aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt
    20281 attggctaca taccagccga catcaggttt acactcgact acacaattga cgattcctga
    20341 gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc
    20401 aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg
    20461 gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc
    20521 ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt
    20581 atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc
    20641 ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat
    20701 ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc
    20761 tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg
    20821 tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca
    20881 cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg
    20941 tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc
    21001 ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt
    21061 tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca
    21121 ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt
    21181 aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc
    21241 aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac
    21301 acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc
    21361 tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt
    21421 gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga
    21481 tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga
    21541 tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat
    21601 cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca
    21661 aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta
    21721 gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca
    21781 gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct
    21841 tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt
    21901 ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta
    21961 caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa
    22021 atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc
    22081 gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta
    22141 ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg
    22201 attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca
    22261 caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca
    22321 atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa
    22381 actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag
    22441 ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag
    22501 gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa
    22561 tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga
    22621 ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga
    22681 tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg
    22741 gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc
    22801 atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg
    22861 aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc
    22921 tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc
    22981 aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg
    23041 atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag
    23101 agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg
    23161 atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg
    23221 aaatgatcag cgaagagcat attattcgcg ggatggtggg cagagattta gttgatcgtt
    23281 atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct
    23341 atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag
    23401 gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc
    23461 tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga
    23521 tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc
    23581 gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt
    23641 tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa
    23701 atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta
    23761 gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc
    23821 tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga
    23881 ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg
    23941 aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag
    24001 tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg
    24061 gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa
    24121 agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt
    24181 attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt
    24241 gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta
    24301 ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg
    24361 atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc
    24421 gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc
    24481 gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa
    24541 tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta
    24601 agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg
    24661 ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa
    24721 tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact
    24781 tatatctttg cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt
    24841 gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc
    24901 ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta
    24961 ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat
    25021 gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt
    25081 ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg
    25141 gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag
    25201 gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa
    25261 cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac
    25321 agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata
    25381 tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga
    25441 gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc
    25501 tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt
    25561 aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg
    25621 taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac
    25681 aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc
    25741 agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt
    25801 tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc
    25861 tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca
    25921 gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga
    25981 agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg
    26041 aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc
    26101 tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac
    26161 acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt
    26221 gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa
    26281 agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa
    26341 gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga
    26401 ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga
    26461 ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat
    26521 attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata agaacagcca aacaaaaata
    26581 gttctttata tgttttgttt ggctggaatt attatcgcag atacaagctt ttgaacatca
    26641 ttattcagaa taagggactg tgataagatt tttgtcacag tcccttatgt tttgcaaaaa
    26701 aagtttttct tttttctcgt aaattcggta aaatcaaaag aaaaaataga caatagaaaa
    26761 gagaagctta tgtgggaaaa agtagagaaa aaacaaggac cgatttatcg tcaaattatg
    26821 gaacagatta tgagagaaat cgaaaacgga aggatcaatc ctggaagtca acttgaatca
    26881 gaacgaaaac tagggcaact ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa
    26941 gagcttaggg agcttggggt actaacgagc aaacgtggca gcggatggtt tgttaatcag
    27001 actgaatggg ggaaattcgc tgttccaaga atcgattggc ggcaaatgat ctctcctcgt
    27061 tatgagcaga cggactggta tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca
    27121 tcgtttcttg atttatacgc tagcgagatg ccagatgagc ttttgccgaa ttttgagttt
    27181 ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag aaatggatct tttaggctat
    27241 actccgttac gtaacaaaat caaacggtat ttggaaagta aatttgaatt ggcattttcc
    27301 cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa tctttttgat tttacaaacg
    27361 attctttcag ctggagaggg gatagctgta gagtcacctt ctttttttta ccgattacct
    27421 cttttcaaag cttcaggaac gcgtttattt gggataccga tggatgaaga agggatcgac
    27481 ttagaaaaac tgaaagaatc gatccataaa aataaattga aagcagtgct tatcaacccg
    27541 aattttcaaa accctacagg aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa
    27601 ttgtgtcgac agtatcggct tccaattatt gaagacgatg tatttacgga tctttcattt
    27661 ctaaaccaag aagaagtcct tcctatacga acgattgatc cggataatgt gttatacgtc
    27721 ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg gttgggtgat aggacctgag
    27781 acttttatct cgcaactagc tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt
    27841 acacaaatca cagtggctaa tgtatttaat gaagcaattg atttgaaaat gcaagatgta
    27901 agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt ggggagattc acaatcgttt
    27961 ttcaagcttc attcaatttt aggtggttac tatgcctgga ttacttggga tgggaaaaaa
    28021 ctagaccccg aactagcaga aaaagtagta gatatgggtc ttggaattgc accaagtcta
    28081 ctttttgggc aagatataaa tggaatcaga atcaattaca gtaggctgga tgaaaaccaa
    28141 ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt ggttaacaga ataatttcac
    28201 aaagtaaata gattaaagaa tataacagct tggatttaaa tttcaagctg tttctttttt
    28261 ttcggaaaaa gaatacgata taaagaaact aagaaaaaac cattatttgt acgtccgttc
    28321 acgatgtgta aataaaataa atgtttgcaa caaaactgta taacaccaac gtttttaaat
    28381 agaaacagat ggttgtaagt ttgattagaa aatagaaaaa atgcagatga aaaataaaac
    28441 gctttcctta ttgtgatttt attcactaat gctgttaata tgcggattgg cataatttcc
    28501 tttcatcgtg aaaatttgtg cttttagctt gtatttctca taaatccatg ataatttatt
    28561 aatgaagacg gttaaatagt gatacagttt tattcactgt ttcaatcgca gaatgaagag
    28621 attttcttat agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga
    28681 cttccaagca ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca
    28741 agatggaaac gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga
    28801 attaatgact catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg
    28861 ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag
    28921 tgcttacgca tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact
    28981 agtaaaacat ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg
    29041 taattcttat ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata
    29101 tgtacaagca gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt
    29161 cacttatact ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc
    29221 tggcgcttac catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac
    29281 acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat
    29341 tcctggtatt caagtcgatg gaatggatcc gcttgcagta tatactgtat ctaaaatggc
    29401 acgggaatgg gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta
    29461 tggaccacat acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga
    29521 cgattggcag aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact
    29581 ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc
    29641 tattgctgaa gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt
    29701 cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa
    29761 ataatcatgg cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta
    29821 gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc
    29881 cgggcaactg aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta
    29941 gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt
    30001 cctgaaatcc aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg
    30061 gctcgtacaa gatatcgaat gagcggcact agaaaactac ccatcacaat tcgttcgcca
    30121 tttggtggcg gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct
    30181 cagtctcctg gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg
    30241 atttcttcta ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc
    30301 tcattccgag aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt
    30361 actcgtgagg gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc
    30421 aaagcagcag acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca
    30481 gttgcgccat tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt
    30541 gttgtccaag aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca
    30601 gaacgcgcta tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc
    30661 ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa
    30721 gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa
    30781 tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc
    30841 aaaccaggag atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca
    30901 gtagaagaaa tcccgtcacc tgttacagga acggttaaaa atgtgattgt tccagaaggt
    30961 accgtagcaa atgttgggga tgtattagta gaaatcgatg caccaggaca cgaagacaat
    31021 gaaggggaca gtggtgtagc cgcagaatcc caaactccag ctaaaccggc tgctgaaccc
    31081 actgttgata cagaatcagc tggatcgtca agcgaaggag tcttccaatt taagttaccg
    31141 gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat
    31201 acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatt
    31261 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
    31321 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
    31381 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
    31441 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
    31501 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
    31561 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
    31621 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
    31681 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
    31741 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
    31801 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
    31861 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
    31921 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
    31981 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
    32041 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
    32101 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
    32161 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
    32221 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
    32281 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
    32341 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
    32401 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
    32461 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
    32521 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
    32581 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
    32641 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
    32701 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
    32761 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
    32821 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
    32881 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
    32941 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
    33001 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
    33061 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
    33121 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
    33181 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
    33241 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
    33301 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
    33361 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
    33421 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
    33481 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
    33541 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
    33601 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
    33661 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
    33721 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
    33781 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
    33841 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
    33901 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
    33961 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
    34021 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
    34081 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
    34141 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
    34201 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
    34261 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
    34321 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
    34381 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
    34441 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
    34501 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
    34561 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
    34621 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
    34681 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
    34741 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
    34801 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
    34861 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
    34921 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
    34981 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
    35041 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
    35101 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
    35161 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
    35221 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
    35281 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
    35341 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
    35401 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
    35461 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
    35521 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
    35581 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
    35641 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
    35701 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
    35761 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
    35821 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
    35881 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
    35941 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
    36001 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
    36061 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
    36121 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
    36181 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
    36241 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
    36301 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
    36361 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
    36421 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
    36481 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
    36541 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
    36601 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
    36661 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
    36721 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
    36781 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
    36841 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
    36901 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
    36961 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
    37021 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
    37081 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
    37141 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
    37201 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
    37261 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
    37321 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
    37381 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
    37441 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
    37501 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
    37561 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
    37621 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
    37681 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
    37741 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
    37801 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
    37861 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
    37921 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
    37981 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
    38041 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
    38101 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
    38161 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
    38221 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
    38281 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
    38341 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
    38401 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
    38461 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
    38521 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
    38581 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
    38641 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
    38701 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
    38761 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
    38821 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
    38881 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
    38941 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
    39001 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
    39061 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
    39121 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
    39181 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
    39241 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
    39301 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
    39361 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
    39421 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
    39481 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
    39541 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
    39601 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
    39661 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
    39721 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
    39781 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
    39841 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
    39901 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
    39961 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
    40021 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
    40081 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
    40141 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
    40201 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
    40261 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
    40321 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
    40381 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
    40441 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
    40501 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
    40561 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
    40621 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
    40681 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
    40741 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
    40801 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
    40861 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
    40921 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
    40981 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
    41041 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
    41101 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
    41161 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
    41221 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    41281 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    41341 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    41401 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    41461 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    41521 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    41581 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    41641 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    41701 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    41761 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    41821 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    41881 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    41941 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    42001 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    42061 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    42121 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    42181 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    42241 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    42301 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    42361 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    42421 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    42481 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    42541 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    42601 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    42661 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    42721 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    42781 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    42841 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    42901 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    42961 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    43021 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    43081 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    43141 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    43201 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    43261 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    43321 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    43381 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    43441 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    43501 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    43561 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    43621 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    43681 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    43741 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    43801 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    43861 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    43921 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    43981 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    44041 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    44101 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    44161 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    44221 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    44281 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    44341 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    44401 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    44461 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    44521 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    44581 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    44641 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    44701 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    44761 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    44821 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    44881 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    44941 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    45001 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    45061 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    45121 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    45181 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    45241 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    45301 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    45361 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    45421 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    45481 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    45541 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    45601 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    45661 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    45721 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    45781 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    45841 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    45901 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    45961 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    46021 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    46081 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    46141 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    46201 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    46261 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    46321 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    46381 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    46441 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    46501 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    46561 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    46621 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    46681 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    46741 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    46801 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    46861 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    46921 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    46981 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    47041 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    47101 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    47161 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    47221 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    47281 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    47341 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    47401 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    47461 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    47521 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    47581 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    47641 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    47701 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    47761 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    47821 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    47881 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    47941 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    48001 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    48061 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    48121 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    48181 agagaaaatg aaatgaaact aaataattaa agaggggttg t
//
LOCUS       DAPCMU010000004        46082 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPCMU010000004 DAPCMU010000000
VERSION     DAPCMU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 46082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.004"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            102..1172
                     /locus_tag="RD744_000190"
     CDS             102..1172
                     /locus_tag="RD744_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556226.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1187..1855
                     /locus_tag="RD744_000191"
     CDS             1187..1855
                     /locus_tag="RD744_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556227.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1875..2453
                     /locus_tag="RD744_000192"
     CDS             1875..2453
                     /locus_tag="RD744_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0556228.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2856..3767
                     /locus_tag="RD744_000193"
     CDS             2856..3767
                     /locus_tag="RD744_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDU0556229.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4023..4940)
                     /locus_tag="RD744_000194"
     CDS             complement(4023..4940)
                     /locus_tag="RD744_000194"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDU0556230.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5142..5579
                     /gene="zapA"
                     /locus_tag="RD744_000195"
     CDS             5142..5579
                     /gene="zapA"
                     /locus_tag="RD744_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDU0556231.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5637..6184
                     /locus_tag="RD744_000196"
                     /pseudo
     CDS             5637..6184
                     /locus_tag="RD744_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            6299..8659
                     /locus_tag="RD744_000197"
     CDS             6299..8659
                     /locus_tag="RD744_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDU0556232.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8746..9060
                     /gene="trxA"
                     /locus_tag="RD744_000198"
     CDS             8746..9060
                     /gene="trxA"
                     /locus_tag="RD744_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDU0556233.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9242..11020
                     /gene="uvrC"
                     /locus_tag="RD744_000199"
     CDS             9242..11020
                     /gene="uvrC"
                     /locus_tag="RD744_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDU0556234.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11165..12064
                     /locus_tag="RD744_000200"
     CDS             11165..12064
                     /locus_tag="RD744_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556235.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12057..13319
                     /locus_tag="RD744_000201"
     CDS             12057..13319
                     /locus_tag="RD744_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556236.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13482..15380
                     /locus_tag="RD744_000202"
     CDS             13482..15380
                     /locus_tag="RD744_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDU0556237.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(15679..16398)
                     /locus_tag="RD744_000203"
     CDS             complement(15679..16398)
                     /locus_tag="RD744_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDU0556238.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            16735..17067
                     /locus_tag="RD744_000204"
     CDS             16735..17067
                     /locus_tag="RD744_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556239.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17054..19057
                     /locus_tag="RD744_000205"
     CDS             17054..19057
                     /locus_tag="RD744_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDU0556240.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19076..19546
                     /locus_tag="RD744_000206"
     CDS             19076..19546
                     /locus_tag="RD744_000206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDU0556241.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19567..20151
                     /locus_tag="RD744_000207"
     CDS             19567..20151
                     /locus_tag="RD744_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDU0556242.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20166..21152
                     /locus_tag="RD744_000208"
     CDS             20166..21152
                     /locus_tag="RD744_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDU0556243.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            21142..21453
                     /locus_tag="RD744_000209"
     CDS             21142..21453
                     /locus_tag="RD744_000209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDU0556244.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21475..23256
                     /locus_tag="RD744_000210"
     CDS             21475..23256
                     /locus_tag="RD744_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDU0556245.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23249..24625
                     /locus_tag="RD744_000211"
     CDS             23249..24625
                     /locus_tag="RD744_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDU0556246.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24632..25267
                     /locus_tag="RD744_000212"
     CDS             24632..25267
                     /locus_tag="RD744_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDU0556247.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25297..26652
                     /locus_tag="RD744_000213"
     CDS             25297..26652
                     /locus_tag="RD744_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDU0556248.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26844..28238)
                     /locus_tag="RD744_000214"
     CDS             complement(26844..28238)
                     /locus_tag="RD744_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDU0556249.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28330..29301
                     /locus_tag="RD744_000215"
     CDS             28330..29301
                     /locus_tag="RD744_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDU0556250.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29298..30080
                     /locus_tag="RD744_000216"
     CDS             29298..30080
                     /locus_tag="RD744_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDU0556251.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30249..30620)
                     /locus_tag="RD744_000217"
     CDS             complement(30249..30620)
                     /locus_tag="RD744_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0556252.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30754..31197
                     /locus_tag="RD744_000218"
     CDS             30754..31197
                     /locus_tag="RD744_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDU0556253.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31240..32049)
                     /gene="yidA"
                     /locus_tag="RD744_000219"
     CDS             complement(31240..32049)
                     /gene="yidA"
                     /locus_tag="RD744_000219"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDU0556254.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32068..33435)
                     /locus_tag="RD744_000220"
     CDS             complement(32068..33435)
                     /locus_tag="RD744_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0556255.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33419..34249)
                     /locus_tag="RD744_000221"
     CDS             complement(33419..34249)
                     /locus_tag="RD744_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDU0556256.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34412..34849
                     /locus_tag="RD744_000222"
     CDS             34412..34849
                     /locus_tag="RD744_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDU0556257.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34925..35545
                     /locus_tag="RD744_000223"
     CDS             34925..35545
                     /locus_tag="RD744_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDU0556258.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35734..36240
                     /locus_tag="RD744_000224"
     CDS             35734..36240
                     /locus_tag="RD744_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDU0556259.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36257..37156
                     /locus_tag="RD744_000225"
     CDS             36257..37156
                     /locus_tag="RD744_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDU0556260.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37243..38127
                     /locus_tag="RD744_000226"
     CDS             37243..38127
                     /locus_tag="RD744_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDU0556261.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38319..38390)
                     /locus_tag="RD744_000227"
     tRNA            complement(38319..38390)
                     /locus_tag="RD744_000227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38355..38357),aa:Arg,seq:ccg)
     gene            38450..39784
                     /gene="rpoN"
                     /locus_tag="RD744_000228"
     CDS             38450..39784
                     /gene="rpoN"
                     /locus_tag="RD744_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDU0556262.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39933..41024
                     /locus_tag="RD744_000229"
     CDS             39933..41024
                     /locus_tag="RD744_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDU0556263.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            41065..42066
                     /gene="gap"
                     /locus_tag="RD744_000230"
     CDS             41065..42066
                     /gene="gap"
                     /locus_tag="RD744_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDU0556264.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            42176..43366
                     /locus_tag="RD744_000231"
     CDS             42176..43366
                     /locus_tag="RD744_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDU0556265.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43470..44225
                     /locus_tag="RD744_000232"
     CDS             43470..44225
                     /locus_tag="RD744_000232"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDU0556266.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44341..45639
                     /gene="eno"
                     /locus_tag="RD744_000233"
     CDS             44341..45639
                     /gene="eno"
                     /locus_tag="RD744_000233"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDU0556267.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        15309 a         7975 c         9828 g        12970 t
ORIGIN      
        1 taatgaaata gcggaagaat tttttgagca taatattcgc caaaaattta tcaagtgata
       61 aaattgttgt ataaaatgat tatttcagga ggaggagtta aatgttttta gcattaaatg
      121 aaattatgca ttcaaagtta cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct
      181 acctggtatt ttttttgact gggttagctt atggcttagc tcaagataat cgaacagcag
      241 tcgacaagtg ggaggcagat tcgattgtgc tttcgaaaga tgcaaattca aatctaggga
      301 tgtccatgat tactaagaag atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg
      361 cccagacacc tggagtcgtg actagtaaag acagtacaga agaaggaaaa atcaatgtta
      421 gcttcttcgg tatcgataaa aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt
      481 ttgataacga tgatgaggca gtaggcgata ttagtttgaa agaagaatat ggtctagccg
      541 taggagatac agttaagtta tctggtagtg acaagacgtt taaactaact ggctttacgg
      601 atcatgccaa attcaatgtc tcaccagttc tctatacaac catcaatgct taccaagaga
      661 tccgcttcga aaaagaagat acaagtgaaa atgcacggat taacgcaata gttgtcagag
      721 gtaaaatcag taatttacct gaagatttag aacagatcaa aatttctaaa ttcatcaatg
      781 aattacctgg atataatgcg caagtattga cttttggatt tatgatcgga tttttgatcg
      841 ttattgcggc tattgtgatc gggattttca tttatgtact gacgatgcaa aagattaata
      901 tctttggtgt aatgaaagca caagggatca caggaggatt tattgcgcgt tctgtagtgg
      961 cacaaacatt cattttatca tttgttggta ttcttcttgg tctattagga acagtgggaa
     1021 catcccttgt tctacctgat gcggttccat tccaaagcaa ttggctgttt tttggcgtca
     1081 ttagcttgtt gatgctggta gtagctgtgt taggcgcact attttctgtt cggacaatcg
     1141 taaaaatcga tccgttgaag gcaattggat aagaaggagg aagagcatga aagcaataga
     1201 atttaaagaa gtagaaaaaa gtttccaaga tggcgaccaa gtagttaaag cattgaaaga
     1261 aacaaccatg agtatcgata aaggggaatt tgttgcaatc atcggtcctt ctggttcagg
     1321 taaaagtact tttttgactt tggcaggcgg attgcagacg cctagcagtg gctctgtatt
     1381 tatcaatgat caagaattca gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa
     1441 agaaattggc tttattttgc aagcttccaa tctggttcct tttctgacag taaaaaagca
     1501 gttattatta gtagataaga tcaaacatga acatcgtcaa aaagaagcaa aagaattatt
     1561 tgagcagctt ggcgtggata agttgatcaa caagtatcca gaagaactat ctggtggaga
     1621 acggcaaagg gtcgcaatcg ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga
     1681 tgaaccaact gctagtttgg attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa
     1741 agaatcaaaa gacaaaaata aagcaacgat catggttact cacgatacac ggctgattga
     1801 caaatgcgat agaatattta tgatggaaga tggtgtattg aaagaaaaag aataaccaaa
     1861 aagcaggtgg aaaaatggtc catcgataca acaaagatac acagcaaaaa attttgaaag
     1921 aagcgactga cttgtttatg tccaagggat atctcggcac ttcaacaaga gagatcgcac
     1981 aaaaagttgg gatcacccaa cccaatctct atcattattt cggtgataaa gaaaaactat
     2041 atacggcagt tttagaaaac cacttaaaag atgttggaaa agctttgaga gaaatcgttc
     2101 aaacaagcga aacgggattt caagtaacct tgacaaaaat ggcgcagttt ttgattgaga
     2161 cacatctggt cgatttgttt ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa
     2221 caagagatca tcttttcttt ctatggaaga aaaattatcg tgaacctttt gaagaaatct
     2281 tttcagcaaa ccaatctgtg ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt
     2341 ttttcttagt gttagcacct tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa
     2401 atgtggcgca attgatcgat ttatatttga atggtgtatt aggagaagcg taaagctgtt
     2461 atatagggag aaaaatagtt aggtgaagaa gcaagatatg acgaagatcg ttatatcttg
     2521 ttttttttat ataggtagtt tttcataaat acaaatttgt tattctctca tgattatcag
     2581 ggtatatttt tatttattat gtcggtgttc caggaagtgt tttaatttaa taacgaagaa
     2641 attaattgtt tgtagaatga aggaaactat ttattgggta gatgaatcac cacgaatagt
     2701 caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc attttagcag taatgttatt
     2761 ttctacggtt atatctttta cagccccttt tttgcgtact taagctgatt ttaagttttg
     2821 acccttagtc tggatctacg aggtgagagt caagaatgaa aaaaataatt ggagcttttg
     2881 tatttgcaat cattatcatt attatctgtt ttcaaataga tgtttttggt caaaaaaaga
     2941 tagttggaca aacggtcagc agtacgaaca gtacggttac aaagacaaag gaatcatttg
     3001 atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag
     3061 atgactcgct aactacccca cttgcaacta taaacggcgg agagctggcg gagtttcttt
     3121 ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg ctatactgga tatttagccc
     3181 tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa accggaagag ctatctgatg
     3241 cagtgattgt attagatcct gggcatggcg gaaatgatac tggggcatta agcaatgatg
     3301 aacaaacaga agaaaaagat ataacattaa gtacagccat aaaagtaaaa aaggcgctag
     3361 aggatgctgg agcaacggtt tatttgaccc atactacaga tgaattagta cagcttggag
     3421 atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt tatcagttta cacgcagact
     3481 ctacggaata tgcgaatgaa gcaactggaa tcacgacata ttattactat ggacaagagg
     3541 aaacattagc acagacaatt gctgactcct tcactgatct gcctctggat tcaagaggaa
     3601 tctccactgg gaactatcaa gttcttcgag aaaatcttca accttctatt ttaatcgaga
     3661 tgggttatat gaataatgat agtgatttaa aggaattagt aacagacagt tatcagcagc
     3721 agattgctgc aagtttgaca caagcattga ccacttattt tcagtaataa ataagcccag
     3781 ccaaacaaaa atagctcctc atatttttcg tttggccggg cttattatcg cagatgcaag
     3841 cttttgaaca ccgtttaagg aagaggttgt gagcctgccg tttaactccg cgtcacaagg
     3901 agcaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg accgaggagt
     3961 tggcatgaga acaccgttta ttcggaacaa gggactgtga catgactttt gtcacagttc
     4021 cattattttt ttagcagctt caaagctttt tctgtattgg caaaatgaag tttggcatat
     4081 ttttcaagca taggcatgcc gcctttctcc aatagttttg ccgctacttg gtcagatttc
     4141 gttccagctc ctgatggaag ggtgattcca gcttcagctg atgctttgtg taactcttcc
     4201 aaaaagctgg cacgactgat gatagaagca gcagcaacag ctaaatgata ttgttctcct
     4261 ttagtaacga agtacagttt ttcttgtaat tgattttttt ctaaacgcgc atatttttta
     4321 aaatttgctt ccggcgtaaa ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt
     4381 ttttttagca gcaaatggat cgcttgattg tgcaaagcga ctttcatccg aacggcattg
     4441 tattttggtt ggatctcatt gtatttttct ggagataaga caagcagttt ataggggatg
     4501 gtttttttta agatgtcagc aagctggatg atttggctat cttttaactc ttttgaatct
     4561 ctaacaccaa gtttacgtaa ttctgcaagc tgatcacgtt tcacataagc agcacatact
     4621 gttagcggac caaaatagct gccatttccg acctcatcac tacctaaaac ggataattga
     4681 ctgatatttt ttggcaaaga agaggctgtt tttgctggct tggcttgttt atcctgtgcc
     4741 tccccccatt tatcagcttc tattgctgca ttcgtacctt gaaacatcac ttttccggat
     4801 gtataagcgg taatggtcgt cccgtctttt tttgcagcaa agctagtata aggaatctgt
     4861 ttatttaaca aataagagcg atagtatgct ttcatttgac taagttggtc gtttgttgct
     4921 ttgataatga cgttgctcat aaaattctcc tcgtttttat acataaaact ataccgcaac
     4981 tcacgacttt tcgttttcta agagattgcg agacatagca tttttttaaa gtttatcata
     5041 taatagttaa gtatgtgaaa aatatttgtg gagaacggtg aaagaaccaa cttcatggta
     5101 cactaaataa aactttcatc aaataggagg aaatgattcg tatggcgcac gaaaaaacac
     5161 gctacaaagc agtgattgcc aaccaaacat atactattat cggacgggaa accaagcatc
     5221 atatggatat cgtaacgaag ctgatcaatg aacagttagc agaattgaaa caattatcac
     5281 cacaaatgga taatgaacaa gcagcaatct tgatggctgt caatgcgctt tcggatcaat
     5341 tgaagaaaca agaaagaatc ttggagctgg aagaagaaac agcagaatta aagaaaaaaa
     5401 tgatcaaatt tacagagctt gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag
     5461 ctcgggaagt attgaaagaa aatggtcaag cagatcatac cattaaaaat catgtggaag
     5521 cacaacagat tttaaacgag aaacgaaaag gacaaattaa acaaaaatct tctaattaag
     5581 atccttttat caaaataaga aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc
     5641 tgagtttgct gattttattt ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt
     5701 ttgctttgca aatcatattc gcgattggat atgtattgtc atttattgct gcccagcatt
     5761 tttacaaacc attagcaagt catttggaat tatacattcc atacccagct gtcaccccaa
     5821 catcaaagtt agcatttttt gatcaggtat ttgcttttca cctagatgaa gctttttatg
     5881 caggaactgc ttttctgatt attttgctaa ttggctggct gcttacccga tttgtggggg
     5941 tttttgtcca tggattgacg tatgtgccga tcttgagaca ggtagactgg attgcaggag
     6001 gcatcctgtc actaatcatg gcttacgtta ctatattttt gattttgcag ttacttgcct
     6061 ttgttccatt agtgtggtcc aaaatcaatt cggcgagcat agtttcgctc gttttatcgt
     6121 tgagagaacg ccatttctta ccaacaagat gtatgagctt tgggtaacga atattttgaa
     6181 ttaaggaaaa tagtgctgga aaatatttcg ttttttgtac agagtagttt atttaagaag
     6241 gctgcgtagc gtatgtccgg cacagctctt ttttcatagt gaaagataag gtgataaaat
     6301 gaataaccga atccttgaaa cattggaatt tgaaaaagtc aaacagatgg tcagacaatt
     6361 tgtagtgact gcacaaggaa aagaagaact ggcagaattg gtaccagtga gtgagaaaca
     6421 aacgatcaca aactggcttc aagaaactga ggatggattg aaagtgcaac gtctgcgagg
     6481 cgggatccct atacctaaac ttgaaaatat ccgtccccat atgaaacgga tcgagatcgg
     6541 agcagacttg aatggagtgg agcttgcaca agtttccaga gtattgtcta cgacaagcga
     6601 attgaatcgt ttcattgatg atctttctga cagtgaaatc gaatttgcac gtttgtatat
     6661 gtgggcagat cagttagtca cgatccctgt tttgagccgc cgtttaaagg aagcaatcga
     6721 tgaagacgga cgcgtgactg atgacgcttc tcctgagtta aagagcataa gacagaacat
     6781 tcggcgaagt gaacaagctg ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc
     6841 aaagtattta agtgatgcga tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa
     6901 acaagaatac cgcggtgtat ttggcggtgt agtccacgat caaagtgctt caggacagac
     6961 tttatttatt gaaccaaaac aagtggtgga tttgaataat cgtttgcgtc aataccaaat
     7021 cgctgaacga aatgagattc aacgtatttt aagtgaactt tctgctgaac ttgttcccca
     7081 tcgtcaagaa atcatccaca atgcttatgt gattggtaaa atggatctga tgaatgcaaa
     7141 agcacgattt ggtaaagaag taaaagccat cgtgccggga atcagtgaag acaatcatgt
     7201 cgtcttgaaa caagcaagac atccactgat cgatcaggaa aaagtagttt ctaatgacat
     7261 cacaatcgga aaagactatc aagccatcgt cattacagga cctaacacag gtgggaaaac
     7321 gatcacgttg aagactttag gtctgttaca actgatgggg caagcaggat taccgattcc
     7381 tgcaggtgaa gaaagtcaaa tcggtatttt tgaggaagta tttgcggata taggagacga
     7441 acagtcgatt gagcaaagtc tatcgacctt ttcttctcat atgacgaaca ctgtagatat
     7501 tttatcaaaa gtcaatgaaa aaagtctggt attatttgac gagctcggtg caggaacaga
     7561 tccgcaagaa ggtgctgctc tagcaatcgc aatcttagat gatcttggta aaaagtctgc
     7621 ttatgtgatg gcaacaacgc attatcctga attgaaagtt tatggataca atcgggcaaa
     7681 tacgattaat gccagcatgg agttcgatgt ggatacacta agtccaacgt atcgtttgct
     7741 gattggcgtt cctggacgaa gcaatgcgtt tgaaatctct agccgattag gtcttgatac
     7801 agaagttatt gatgaagcga agcagttgat gaatgatgag agccaagatt taaacgaaat
     7861 gatcacagat ttagagaatc gccggaaaat ggcagagacc gaatatctgg aaatgcgtca
     7921 ttttgtttct gaggcacaag aacttcacga tgatttgaaa gaagcttaca gctatttctt
     7981 tgaagaacgt gaaaaagaaa tggaaaaagc aaagaaaaaa gcaaacgaag tcgtgtcaga
     8041 ggcggaagaa aaagcagaaa aaatcattgc tgatattcgt aaaatgcaac agcagattgg
     8101 gcaaggaaat gtgaaagagc atcagttgat cgatgcaaag acccaattag caaatctaca
     8161 tcaagaagaa actttgaaga aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt
     8221 gaaaccggga gacgaagtgt tggttacgac ttacggccaa agagggacgc ttttacgtaa
     8281 aaacgggaat cagtggcaag tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga
     8341 gctgacgcct gttgctcctc aaaaagagcc gacacaacga gttatccatg ctgtacgatc
     8401 tgaatctagt agtcacgtgc cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc
     8461 gctaagtgag gtagatcaat atttggattc tgccattcta gctggttatc ctcaagtgac
     8521 tattgtccac ggaaaaggaa caggcgcact tcgcaaggga attactgatt atcttaaaaa
     8581 ccatcggagc gtcaaaagct ttgagtttgc accggcaaac caaggcggaa atggtgcgac
     8641 aattgttaaa tttaagtaaa gcttactaaa ggtaagcaga tggttcgcta tccaagtata
     8701 gaaatgctat actaatcata gattttaaaa atggaggtct ttgatatgtc acaagtaatt
     8761 acagataaga catttaatga agaaacagat aaaggacttg tattgattga tttttgggca
     8821 acttggtgtg gtccatgtcg tatgcaagca ccgattttgg atcagttgga acaagaatat
     8881 gatgaagaag aattcagaat tgctaaaatg gatgtggatg aaaatccaga aactcctcaa
     8941 caattcggta tcatgagcat tccaacatta atgttgaaaa aagatggtca agtagtagaa
     9001 aaagctgttg gtgttcattc aaaagaacaa ctacgtcaaa tgatcgatca atatttataa
     9061 aaacttacag caaaaataag ctgagggtgg gactgaatcc agttccgtcc tcggtttttt
     9121 tgcggttgag ttaaggttat tcaaattaat attttttgag tataattacg ttgtcaggct
     9181 ggattgtttt tttgacagac atgctataat aactttaatt gaatatgaag gggagtacca
     9241 gatgaacgaa cgaatcaaaa acaaattagc tttacttcct gatcagcccg gctgttactt
     9301 gatgaaagat aaaaatggaa cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg
     9361 tgtccgctca tattttactg gcagccataa tacgaaaacg gaacggcttg ttagtgaaat
     9421 cgaagacttt gaatacatcg ttacagaatc gaatatcgaa gcattgctct tagagatcaa
     9481 tctgataaag aaaaatgatc ctaaatacaa cattatgtta aaggatgata aaacctatcc
     9541 tttccttaag ataacgaatg aaaaatatcc ccgtttggtg attactcgta aagtactaaa
     9601 agacaaagca ctgtattttg gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa
     9661 gatcctcgat cgattatttc ctctgagaaa atgcaaacct agtcagacga aggagccttg
     9721 tctttactat catttgggac aatgtctgtg tccttattat ttcgacgtag atccgaaagt
     9781 ctatacgaaa atcgttgatg aagtgaaaaa gtttttaaac ggaggacacg aaaaaatcga
     9841 gtcagagatc catcagaaaa tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc
     9901 agaataccga gaccagatcc gtgccattga gacaatcatg actcgccaaa aaatgacaaa
     9961 tactgattta ctggatcgag atgtatttgg ttatgcgatt gacaaaggat ggatgtgtgt
    10021 ccaagtgttt ttcgtaagac aaggaaagct aattgaacga gatgtttcaa tgtttccttt
    10081 ctataacgaa gcagaagaag atttcttgac ttacctcggt cagttttatc aggaaaatga
    10141 gcatttcatt cctaaagaag tcttgattcc tgatacgatc gacaagccca gtgtagaagc
    10201 tttgttagct acgaaagtgc tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt
    10261 ggcgaataag aatgcctcag ttgctttgaa tgaacggttt gatctgattg ccagaaaaca
    10321 ggaacggacg atcggagcag ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat
    10381 acgtatcgag tcttttgata actctaatat catggggacg aatcctgtgg ctgcgatggt
    10441 cgtattcatt gatggaaaac cggcaagaaa agaatatcgg aaatataaaa taaaaacagt
    10501 caacggaccg gatgattatg catcaatgag agaagtcatc tatcgcagat attcacgagt
    10561 gattcgcgaa gggttgcctt tacctgattt gatcttgatc gatggaggaa aaggacaagt
    10621 agatgtggca aaagatgtct tagaaaatca attaggaatc gatattccta ttgctggcat
    10681 ggcaaaaaac gataaacata aaacaaatga gttgctattc ggtatagatt tggctgtagt
    10741 accgcttgag cgaaattcac cagagttttt cttactccag cgaatccaag atgaagtcca
    10801 tcgattcgca atcactttcc atcggcagct acgcagtaaa aacagttttg cttcccgatt
    10861 agatgaaatt gaagggttag gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc
    10921 gttgaaaaat atcacagcag ccacgacaga agaattacaa gaaatcggac tgccgaaaaa
    10981 tgtcgcacaa aacgtatttg ataaattaca tcaggaatga ggaaagggca ctttacaaag
    11041 aaagtaaagt gtcctatcat tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa
    11101 ataataaatc gcttacgtct ggtgtctaag gatatatgtg atttcttgaa gaaggagtga
    11161 agaaatgtta gaagtcaagg atcttgttaa aacttttggg aactacacag ctgtcgatca
    11221 tgtctctttc caaatccccg atggaaaaat catggggcta attggccaaa acggggctgg
    11281 gaagacaacg acttttcgtt tgatcttgga ttttttgaca caagaccaag gagaagtgtt
    11341 atggaatggc catcatcttg gggagaaaga ttatgatgtc atcggttatc ttccggagga
    11401 acggggactt tatccgaaag taacgattca agaccaactg ctttattttg cagaacttag
    11461 agggaaatca agaaaagaga ttgaaccaaa aatcgatttt tggatggaaa aatttcaggt
    11521 aaaagggaaa aagacggata aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca
    11581 gctgatcgcc acgttaatcc atgaacctaa attgatcatt ttggatgagc cattcagcgg
    11641 gttagatccg gtaaatgctg aattgctaaa agatggaata ttggaattga aaaaacatgg
    11701 ttcatgtgtc attttttcta gccacaatat ggataatgta gaaaaaatct gcgatcattt
    11761 gatcatgttg cgaaacggaa aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc
    11821 atttggccgt acgaagttat tcttggaatc tggtttaagc caacaggaag tagaagaaat
    11881 cgaaggcgtg aaaaaagtga tccttcatga agacaatacg ttagagatta ccttagaaga
    11941 tcctgaagct gggaaagaaa tctttactcg tgcgactcag ttcggctaca ttcctatgtt
    12001 taaccagcaa ccgccaactt tggaagagat tttcaaattg aaagcaggtg aaatcgatgc
    12061 gtaagttttg gattattacc aaagatgtct atttaaaaaa tgttaagtcg atttctttct
    12121 tgatcatgat attagtccct tttatcatgc tgggtatcat ttatgtagca ggaaactttg
    12181 ctcaacaaaa tagtgatgtc aataaaatcg gcgtattggc tgataatgag caaatcagtc
    12241 agcaaatgag ccaagtcaaa acagatgatt ttgagttcaa agcagttgat tctgaagaaa
    12301 aagcacaaaa agagctttcg gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga
    12361 atgataagat aaatgggatg ctttatagcg aaaattcatt aggacaaagt acacaactac
    12421 tgatacaaca acaactgact ggcttgcaat cgatgcttcg agcaagtcaa ttaggcattt
    12481 caccagaaga agtagctagc ttgagccaac aagcaagttt gactcgacaa aaagtcaatt
    12541 ttgattcaaa aggaaaaatg acagttggag aagataactc gacagtccaa tatgtcgtaa
    12601 gttatgtagc aacgattgtc ttgttcatta ttattttgac gtatgcgcag atcattgcac
    12661 aagagatcgc ttctgaaaaa ggcacgcgga tcatggaagt gatcctgtcc agtacgactg
    12721 cacaaaagca tttttacgga aaactaacag gtgttttact tgttgcatta acgcaaatgg
    12781 cattatacgg tattattttt ggtataggat acagtcaatt caaagatatg gaaatggtca
    12841 aaaatatatt agacggtatc tcattggata acttgattgg accattcttg tggtattctc
    12901 ttctattcat gatctttggc attttgattt tttctgtttt agcagcttta tgtggctctt
    12961 tggtcaacaa agcagaagat acagcaaaag caattttgcc agtgacttat ctatctctag
    13021 gcggatatat gcttggattg atactaggag cttcagatcc aaacaatatc gttattcgga
    13081 tcacttcgta tattccattc ttatcttcat atattatgcc ggttcgctta gcaaatgaga
    13141 cagtcggtat gggtggagca ttgatttcct tgttggtact tgtagccgta acattcttat
    13201 tgatgatctt gtctgcaaac atgtacaaat cgaatgtact cgtgtatagc gaaggcggat
    13261 tatggagttc attgaaacaa tcgatttcaa ttatgagaaa tgaacgaaaa aaagcataaa
    13321 atatagaaaa atatagaagg aagctggaca tttattgttc ccagcttctt ttttgtatag
    13381 acctatttct atttttttat tatattggat atatatattc attgaaaata gagcataaca
    13441 actttataat gttgagtata ccaatttttg gaggcgtttt aatggaccgc tatttagatt
    13501 tactttcaga gaagttccct acaacggaag atgtcatcac ggaaatcatc aatctcgaag
    13561 caattttgca gcttccaaaa ggaacagagt tatttttgag cgatatacat ggcgaatttc
    13621 ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag atcaaagacc
    13681 ttttctctga acaaatgacg gaaacggaga tcagccaatt cactattttt acggcttatc
    13741 cagaatatgt gttgacgact gattggtaca aaaaacaaga taaatcccag ctgatccatc
    13801 gtttgatcga tcttttacgg tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag
    13861 gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat gatcgtatca
    13921 acgggaaaaa agactatgta aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag
    13981 aaaaattttt aacaaaactt gcgcagacga tccaaaaatc tgtcatcgat catttgcata
    14041 tcgtgggtga tatttttgat cgaggaactc aagcagccaa agtaatggat cgaatcatgg
    14101 atttttcctc tgtagatatc cagtggggga atcatgatat tttgtggatt ggtgcgtatt
    14161 gcggctctga ggcatgttta ttgacgcttt tacggattgc cgcacgctac aactatttat
    14221 ttgaattaga gaaagaatat ggattaaatt tacgtccatt attttctttt gcccatcaaa
    14281 cctatcagaa gaatccagta tttaccccta aaaacaggaa agatctatct gaaagagagc
    14341 aagaagaatt agaaaagatc catcaagcac tttctatcct ccaattcaaa tcagaacatc
    14401 agttgttgaa tcgacgacct gaatttaaaa tggacgatcg taaattgttg gaaaaaatca
    14461 actatgagga atcaacgatc gttttagaag gtcagctcta tgagttgaaa gggacttgtt
    14521 ttcaaacgat ccagccagat gatcctaaaa aattcttgga agaggaacaa aaagtgatcg
    14581 atagtttgat gtattcattc cagcactcgt taagaatgca aaaacacatg acgtttttga
    14641 tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt actgttccat ggctgtatac
    14701 ctgtagatga aaaggggcag ctcttagcat ttgaattaga ccaatcttat cgcggaaaag
    14761 aactgctggg attttttgaa aaacagataa ctgaaagcgc aaaagatttg actgcaaatg
    14821 aagatttggc gaccgatttg atttggtatg cgtggagcgg gccattttcg cctttattcg
    14881 gcaagagtaa aatggcaact tttgagcgct attttctcac agagcctcat acgcacgtcg
    14941 aaaaaagcaa tccctattat cacttgcgag atgaagagtg gttcagtgaa aaaattttgg
    15001 cagaattcga tgcaaaagaa gaaggttcag caatcatcaa tggacacacg ccagtaaaag
    15061 taaaaaaagg agagtcaccg atcaaagcaa acggaaaagc atttgttatt gacggaggtc
    15121 tatccaaagc ttatcaaaaa acaaccggga ttgctggcta ctcattacta tgcaattctt
    15181 acggtttcca aatcgtgaca cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa
    15241 aatctgatca aacgaatgta aaaaaagtga tcgagcagca gctcccacgc aaacttaatc
    15301 ggaatacgac aaaaggaatg gaattacagc aacagattac tcaactccaa aggctgttgg
    15361 attatcgaaa ggacgattga gaaaaatgct agccaacatt gctcaataga ctaaaaagcg
    15421 actgcaacaa aaggcgtgtc gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat
    15481 tagaagctat ttttctgttt atttctctaa tactcaggtc agggcgctga tttcacgacc
    15541 tcttccttaa acggtgttct cctgccaact cctcggtcac aagtcacaat atccaacaaa
    15601 catgagaagc tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg
    15661 acctaatcag aaaaacgatt aaaattcatt cacaaagaaa tcgaataaac gctgttcaga
    15721 tgaatctaca cgatgagaaa gttccggatg ccattggaca ccaaggattt tgtgcatatt
    15781 atctttactt tggactgctt caatgagtcc atcgttggaa aaggcgatgg cttttaacaa
    15841 aggagaaaga tctttgactg cctgatgatg ataggaattc acaaagtatg aatcagggag
    15901 aaggtcactt aataaactgt ccgaaacgat tttgacttcg tgagttgcaa actgcggggc
    15961 agtgggctgt tgttcgtgtt ttacggacca ttttggatat aatgaaagat cttgataaag
    16021 tgttccctcc aacacgacat tcaatagttg aagtcctcgg cagactgcaa aaatcggttt
    16081 tctttgtttc aatgcttcca agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt
    16141 ttccgtcagt tttggatgag ggtcttccat gtaaagttgc ggactaacat cctgaccgcc
    16201 tgcaagaatg agtttgtcga ttttctcaat tagttgaggc gctgaatctg gagacatgac
    16261 tggcagaata agaggtaatc cttgagcatc taaaactgca tccacaaatc cctgcggtgt
    16321 atagcttact tgatttcctt ggaaagtatc tgtcgctcga atcagttgat tcccggcaat
    16381 gccgatgatt ggtttcattt ttattccccc taaagtagac taattgtaac aaggatttaa
    16441 aaaaatttaa actatgtaag acttgcccct atacttatct gttcttgctg tgttactatc
    16501 tgtaaagaga attttcgtgt gaaattcatg acgatagttg aaaatattgc ttaatttgtt
    16561 ataataaaaa gtaagcagaa ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc
    16621 aaggggtgag aaggacattt tattgaaggg gcctgcctat actaagttat ctttagaggt
    16681 acatactttc aatgaaaaaa gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag
    16741 caagtgttag aagctttgga acaaatccga aaagcagaag aagaaaatga gaagcgtgct
    16801 cgaaccctac ggcagcagtt gaagacctat gcagaagaaa aagagcaaga attggagata
    16861 atccaaacga acagtcggca acagctgaaa cagttggtag aagaaaaaga acaattcgaa
    16921 aagcaagaat tagaaaaaga aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca
    16981 actttgaaag accaatatca aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg
    17041 gtgaaagaaa cttatggccg tcactaagat ggaaaaagtg accctcattt cagataaaag
    17101 aaatcaggag aggattttac aagcagtaca aggaatccaa aatgttgaaa tccgggatct
    17161 gtatcaaaat agaacaaaca accagtgggt agaaaaatat tttccagata ccacgattat
    17221 tgataaagaa gcaaaattaa gcgctttagg caatcggctg aaagacatcc aagaagctat
    17281 ccaattcata caacaccacg gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga
    17341 actttcctta caagacttgg aagcaaatta ttccgaagaa aatttcatga ataaattaga
    17401 agaagtcctt gatttaaaag aaaagtggga acagctaact gaaaaacgtg atcgtttaga
    17461 agaagaggaa gaatggcttc ttcattggca acagctggat attgctccaa gtatgtatgc
    17521 aagtaagacg accatttttg agatggggac tgtgagtgca gcaaattttg aaagtttcaa
    17581 ggaagaacta agtaagcttg atgacgttta tatggaagaa atagattata cgccaaaaca
    17641 agtttatctt tcgtatgttg ctttaaaaaa atcagttgct gctatagaag aaattgctgg
    17701 gaaatacggg ttcaataaag aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca
    17761 agaaaccaaa cagcaattat ccattgttca cgagcagcaa aaaaagataa cgacagcgtt
    17821 aggtcagtgc agcggctata tccaagaatt tgaatgggta gaagaagtca cacttgccgt
    17881 tgcagaacgg gaaaaagtaa aagaacggtt tatccatgcc acttatctga tcgttttgca
    17941 aggttgggta gatgcagaag aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc
    18001 agaagaagat atatacgtta gtttcgaaac accaaactca gaagagattc agcaggaagt
    18061 ccctactaaa ttgaaaaacc atccgcttat cgaacctttt gaattgctga ctgaaatgta
    18121 cagtctgcca aaatatgaag aagtagatcc aacgccttgg atgacaccat tttatcttgt
    18181 cttcttcggg atgatggtcg cagatgttgg ttacggattg ctgatgctca ttgggacaat
    18241 actagctcaa aaactatttg ttttaccccg cgggatgaaa cgtttcgtaa aattctttga
    18301 gattctctcg gtaccatcga tcatctgggg attgatctac agttcatttt tcggacaggc
    18361 attaccaaaa gaactatttg gtatccgctt gcctttccca atcctatcta cgacggatga
    18421 cgtcaatacg attttgattt tatccgtgat tttcggtctg atacagatct tggttggatt
    18481 attcgtggca gcaaaggaaa atatcaggag gaaagattac ttgagtgctg tcagtgatgg
    18541 ttttgcctgg caagggatcc tgattgggat cgttattgca ttgattggtg tcatgttgat
    18601 aaaaaataca gcatttgttt atcttggcgc ggggctatcg atcattgctg ctttatgtat
    18661 cttaatcgtg ccgattatcc aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta
    18721 taatctttac ggattgactg gttatatcgg tgatttagtc agttatacac gtttgatggc
    18781 actaggtatt tcaggcggaa gtattggcgc ggcattcaat atgctagttg ctttcatgcc
    18841 gccagccgct cgattcagtg tagggatcct tttgatcatc gctttacacg cactgaatat
    18901 gtttttgaca ctgttaagtg cctatgtcca tggtgcacga ttgcagtatg tcgaattttt
    18961 tggaaaattt tatacaggag gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata
    19021 cgtcaacatc aatcataaga aaaaaaacaa taaataattg gaggattttt atacaatgat
    19081 ggattactta attagtcaaa atggtggaat ggtatttgca gtattagcga tggcaacagc
    19141 gacgattttt tcagggattg gttcagctaa aggtgttgga atgacaggtg aggcagcagc
    19201 agcgttaaca acaagtcaac ccgaaaaatt cggacaggct ttgattctgc aattgcttcc
    19261 aggtacgcaa gggttgtatg gattcgttat cgcctttttg atttttatca acttaggaaa
    19321 tgatatgtca gttgtacaag gattgaactt tctaggcgct tctctgccga ttgcttttac
    19381 aggtttgttt tcaggtatcg cccaagggaa agttgcagca gcaggtatcc aaattttagc
    19441 gaaaaaacct gaacatgcta caaaaggaat catttttgct gcgatggttg aaacatatgc
    19501 gattttaggt ttcgttattt ccttcctatt agtattgaat gcctaagcat aaaggaggaa
    19561 aagcagatga acgccattga aaaaatcatc tctcagatga atgaagcagc agagcaagaa
    19621 agagcggcgt tagagcaaga agagcgaatg aagatcgatc aaaactttga acaaaaacgt
    19681 acgcaaatcg aaacagaaca tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa
    19741 aaataccgtc agcttaggaa tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg
    19801 aagcaggaat ttttacgacg attgtttgca gatgcaatag ctgaaatgga gaattgggat
    19861 gaatctgaac aaatccagtt tatcaaaaat tcattgtatt cattaccttt gacaggcaaa
    19921 gtaacattca ttgctggaga aaaatcagca gcttatcttt ctcagacatt gctggatgaa
    19981 tggaacaatg aacttccctt tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg
    20041 ggttttctga taaacgatca aggagttcag tataattttc ttttcagcag ccttgtacaa
    20101 gatatccaag gaacgatgag ttttgagatt gccaatcaat tattcgaata aggagggatc
    20161 agtgtatgga ataccacgaa ctaaatccct tgattcgcgg aagagagctt gaacttttgt
    20221 ccaatgagac atttgaacgg atgattcaaa caaattctgt cgaagcactt ggtgaaatat
    20281 tgaaatccac gatttacagt ccatatattt atgacggata tgaatatgac ttcgaacaga
    20341 atcttttaaa ggagcagagc cgtctatttc gctggttaaa ggaatcagca ccggagcctg
    20401 aaatcgtttg gatctatacg atgcgctata ctttccataa tctgaaggtt ttgacaaaag
    20461 ccgagatgac aaatcaaaat ctggaccatt tgtatatcga cgatggattt tattcattag
    20521 aaaccttgaa ggatgccatt catacgcaag tttcaaatga actccctgaa agcattatgg
    20581 acagtatacg agaagtacgt gattattttg aagagtctac cattcttcag ggaattgacg
    20641 tgatttatga tcgacagttt ttgacagagc agcgacggct tgccgataag ctaggatatc
    20701 aagaattgct gacagaagtc attgctttta ttgacttgac caatatcaca acgatggctc
    20761 gaggaatatt gcagaaaaga acatatgggt tcatgacgac ggtactttcc agctcgggaa
    20821 gtatcgaaaa agaagtattt ttgaactttg tagataaaga tatggaagat tacacgaatt
    20881 ttctattaga gacagattat gctgattttc tgcgccctgt catccgtgag ggaaatcttg
    20941 atctcgtaaa gcttgaacag ttgaaagatg attatctaag ttcattgtat caagaagcac
    21001 aaacccaggc tttcggacca ctgccactac tagccttttt gaacgcaaaa gaggtagaaa
    21061 gcaaaaacct tcgtttactg gttatcggca aaagaagcca tttcgaacca gaaacaatca
    21121 gaaagcgggt gagacaagtc tatgacgcat aagatcggtg tagttggcga caaagattct
    21181 gttttgccat tcaaattgtt tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt
    21241 cgtcaggcaa ttgaagaaat ggcaaagaat gagtatggcg tgatttatgt cacagagcaa
    21301 tgtgcagcat tgagcccaga aacgatcgaa cgttacaagg aacaaatgat acctgctatc
    21361 gtattgattc ccaatcatca aggatcatta gggatagggc tggaagaaat tcaaaaaaac
    21421 gtagaaaaag cagtaggtca aaatatctta tagtatgaaa aggagataga aattttgcaa
    21481 attggaaaaa tcataaaagt ctccggacct cttgtaatgg ctgagaacat gtctgatgcc
    21541 agtatccaag acatgtgtct agtgggagat ctaggagtta tcggagaaat tatcgagatg
    21601 cgtggcgatg tggcgtctat tcaagtatat gaagagactt ctggaattgg tccaggcgaa
    21661 ccggttcgct ctactggtga agctttatct gttgaattag gaccagggat catttcacaa
    21721 atgtttgacg gaatccaacg gccattagat acctttatgg aaatcacaca aagtaatttc
    21781 ttagggcgcg gagttcaatt gcccgcattg gaccatgaga aaaaatggtg gttcgaacca
    21841 acagtagaag ccggagaaac ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca
    21901 aaagttataa aacataaaat catggtgcca aatgggataa aaggaacgat caaaacaatc
    21961 gaaaaaggtt cattcactat tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac
    22021 aaaaatttga cgatgcttca aaaatggcca gtaagacgta gtcggccaat caaagaaaaa
    22081 ttaaacccgg atgcacccat gatcactgga cagcgagtga ttgatacatt cttcccagtg
    22141 acaaaaggag gagcagcagc tgtacctggt ccttttggtg cagggaaaac agtcgttcag
    22201 catcaaattg caaaatgggc cgatgtggac atggttgtct atgtgggatg tggcgagcgt
    22261 ggaaatgaga tgacggatgt tttaaatgaa ttccctgaat tgattgaccc taatacagga
    22321 gaatcgttga tggaacgtac ggtattgatt gctaatacat caaacatgcc agtagcagct
    22381 cgagaagctt ctatctatac gggaatcaca attgcggaat atttccgtga catgggttat
    22441 gacgttgcta tcatggcaga ctccacttca cgatgggcag aagcacttcg tgagatgagc
    22501 ggacgtttgg aagaaatgcc tggtgatgaa ggttatccag cttatcttgg ttcacggctt
    22561 gcggaatact atgaacgatc tggaagagtc attgcattag gatcagaagg tcgcgaagga
    22621 agtataaccg caatcagtgc tgtatccccg tctggagggg atatttcgga accagtcaca
    22681 caaaatacat tgcgggtcgt aaaagtattc tggggtcttg attccagttt agcccaaaaa
    22741 agacattttc cttcgatcga ttggatacaa agctattctc tttattcaac agaagtaggg
    22801 aagtatatgg atcatatcct tcaagctgac tgggcatcca tggtcaccga gggcatgcgt
    22861 attttgcagg aagaacagca gctgaatgaa atcgtgcgtt tggtagggat tgattccctt
    22921 tcagataatg accgtctgac attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag
    22981 caaaatgctt ttgatgatgt cgatacattc acttcaagag agaaacaatt caaaatgttg
    23041 aagatgatct tgacctttgg tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta
    23101 agtgagatca tggacggtac cgtatcgatt cgtgaaagga tcggacgaag caaatatatc
    23161 ccggaaacag aattagaaaa actagatcaa attattggtg aaatcaagca aacgatgcaa
    23221 acgatcgttt cagaaggagg catgagcaat gattaaagaa tatcgtacga tcggtgaagt
    23281 cgtcggtcca ttgatggcgg ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga
    23341 agtccgtatg caaaatggcg agattcgtcg tggacaagta ttggaagtcc aagaagataa
    23401 agcaatggtc caaatattcg aaggaacaag tggtatcaat ttacgtgact cttctgtacg
    23461 atttttaggc catcctttag aactaggtgt atcggaagac atgatcggtc gagtattcga
    23521 tggtttggga cgaccgaaag ataacggacc agagatcctg cctgaaaagc atttggatat
    23581 taacggagaa gtaatcaatc cagtagctcg ggattatcca gatgagttta tccaaacagg
    23641 tatctctgca atcgatcatt tgaatacatt agttcgtggg caaaaattac cagtattttc
    23701 tggctctggt cttcctcata aagaattagc tgctcagatc gcgcgacaag caacggtact
    23761 cgattcatca gatgatttcg ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc
    23821 tgaatttttc atggaagatt ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat
    23881 gaatttagca aacgaccctg caatcgaacg tatcgctact cctcgtatgg ctttgactgc
    23941 tgcagaatat cttgcttatg aaaagggaat gcatgttttg gttatcatga cagacatgac
    24001 caactatgca gaggcattgc gagagatttc tgcagcacgg cgagaagttc caggaagaag
    24061 aggatatcct gggtatcttt acacgaactt agctacttta ttcgaacgag caggaagaat
    24121 ccgcggacta aaaggatcgg ttactcaaat tccgattcta acaatgccag aagacgataa
    24181 aactcatcca atccctgact tgactggtta tattacagaa ggacagatca tcttgactag
    24241 agagctttac aagagtggga ttcagccacc aattgatgta ctcccgtctc tttcgcggtt
    24301 aaaagataag ggaactggac ccggaaagac aagagaagac catgcagcta cgatgaatca
    24361 gctttttgca gcctatgcac aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga
    24421 atctgcttta tctgatgtag acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga
    24481 atatgtcaat caaggattct acacgaaccg ttcgattact gaaacacttg atctagggtg
    24541 ggaactgcta tcgatgttgc caagaaccga attaaaacga atcaaagacg acatgctgga
    24601 caaatatctg ccagaaggga agtgagttct gatggcacga ttgaatgtga atccgacccg
    24661 catggaattg actcgattga aaaaacagtt gacaactgcc acaagagggc ataaattatt
    24721 aaaagataag caagatgaac tgatgcgtca attcatcctg ttgattcgaa agaacaacca
    24781 gctgcgtcaa gaaatggaag agtctatgac agaagcaatg tctgattttg tattggcaaa
    24841 tgcttcgatc acggaacctt tcatcgaaga attatttgtt ttgccggcgg aaaacgttga
    24901 tttgtctatc gtagacaaaa atatcatgag tgtaaaagta cctgtgatga attttgaata
    24961 tgatgaacag ctgacagaca cacctttgga atacgggtat ttgaattcca gtgccgaact
    25021 ggatcggtca atcgatcggt ttactaccct gcttccaaaa cttttggaac tgacagaaat
    25081 cgaaaaaact tgtcaactga tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc
    25141 attagaatat atgacgatcc ctcaattaga agaaacgatc tattatattc gtatgaagct
    25201 ggaagaaaac gagcgcgcag aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac
    25261 tgactaagtg gtatcttttt ttaaaagagg aaaacgatga ttaaacaaag caaacaaata
    25321 aagaaacgtt tgccggctgt tcagcttctc gcattaggat tttttctcct gattttagtt
    25381 ggcggcggcc tacttaccct accatttttt agtaatagtg ggcatgggac aaattttatc
    25441 gatgcgcttt tcactgcaac ttctgctgtc tgtgtaacgg gattgacaac attgaataca
    25501 gcagagcatt ggaacagtgc tggtcaattt ttgatcatgc ttttgattga gatcggcggt
    25561 cttggtttta tgatggttcc gattctatgc tttgcattga taaagaagaa agtcagtctc
    25621 agcacacgta ttatattaaa agaagcgctg aatctagaag aagtatcagg tgtgatgaag
    25681 ctcatgcttt acatcctaaa attggccttt gttatccaat tgattggtac ggttgcttta
    25741 tcttttgtat ttgtgcctga atttggctgg ttgaaaggta tctggtacag tttgttccat
    25801 gcaatctcta gtttttgtaa tgcagggttt gatttgttag gagacagttt agccaatcac
    25861 cagcaaaatg tttatttgat catggtggta tctgctttga tcatttctgg tgggttaggc
    25921 ttcatcgtat ggcgtgattt actggaatat catcagaaaa agaagatgac gattcattca
    25981 aaaatcgcat taaccgttac aggtaccctt ttattaggcg gattcgttgt gttctttttc
    26041 acagagcgca atggggcaac gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc
    26101 ttttttatga gtgtgacacc tagaacggct ggatattatt cagttgatta cctccatatg
    26161 agccatgcag gtctgatttt aacgatgttt ttgatgtaca tcggcggaac atctgggtcg
    26221 acggccggcg gattgaaaac aactacgtta ggtgtgttgc tgatccagat gcgttcgatg
    26281 ttgaaaggac gtacgagagc ggaagcattt ggccgtacca tccgacaagg cgcagtgttg
    26341 cgggcactta ctttattttt tgttacgttg acgttgtgtg tagtcgccat catgatttta
    26401 tcggtgactg aaacgatacc taaaacttct ggcatcgaat atattgcttt tgaggtcttc
    26461 tctgcgtttg gtacagtagg tcttacgatg ggattgacgc cagacttgac tgcaataggg
    26521 aaattgatca ttatagcgct tatgtacatt ggacgagttg gtgtattgac tgtggtcttc
    26581 tctctgatga caaaagcaca aagaacacaa tcaaaataca aatatccaga agaaagtgtc
    26641 atgattggat aatttcatct tctgacagaa acctagtgat ttagtaaaat aagaaaataa
    26701 aataattttt cttgcttctt ggatacttta agtgcaaaat gttcgtttat tttttgaggc
    26761 actacccgtt gttgaacctc gggtagtgcc tcttttttat tgaaaggaat caataagatt
    26821 tgattccttt atttatcaat aggttataat tcatattttt ttattttttc ttttacgata
    26881 cttagtccag aagctaaaat ttcaaggtta ttagaggata gggaaattcg aacgaatgca
    26941 tctggttgcg cacgtgtagt aaagcgtctg gaatgataaa gtcggatacc gttagcaaga
    27001 aagtcattct caattatctt agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa
    27061 gggacacgat tcgttggttt ttctactttg aaaagagaat caaaaagctg attggctctt
    27121 tttgtaagac ttaatttagt ttctaagatc ctatttgctt catttgaatg gaggatttga
    27181 gtgatgactt ccacatctaa tccagaagtt ttcacattta tattaaaaat tgcttgttct
    27241 atcttgcttt ggacatgtac tggaactaca agataagcaa ctcgcaatcc agaacaaata
    27301 aattttgtca ttccagctaa gtagattgtt tggttaggta ataaattttg gaaaggagga
    27361 atgatcgtat gttgatagta agttgttaaa aatgaatgga tatcatcttc aataacccat
    27421 agaccttctt tttcaatcac ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac
    27481 cctataggat tattacaaga aggcataaga aagacaccat gaatcttctt ttttctacat
    27541 tcctgaatca atatatcagg tcgcattcct tgctcgtcaa aatcaattgg aacgatctct
    27601 aaatgatata actgagccaa ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt
    27661 ctatcacctg gagagaaaag tgcagaaagt gtgatagcca atccattttg aacaccggaa
    27721 acaatagata gagattctaa ggacggaatg actccttgag tttttaacca atttgctgca
    27781 gtagtcaatt gaaaatttgt gcctttggga tcttggtaat taagcaaacc aacaagattt
    27841 gggttattac tagttgattg aataaacggg acaacgaatt tattacattc ttcaaaagaa
    27901 ctaaccagac caaaatcaat gatatgttcg gttacttcat tggtagagat ggtgatggac
    27961 tcaaagacat tgggagaaat aaatgtgcca cttccaatat tcgtatacag caaacctttc
    28021 tcaattccat atttataagc cttgccaacg gtagtaaaat ttaaatctaa ataatcagct
    28081 agctctcttt gggagggtaa ctgagtgttt ttctgtaatt tattctttaa aatatcttgt
    28141 tctaattgtt gaatcaacga tcgataataa ggacgcttca atttttgttt gtctggtcgc
    28201 caagttaacg ggtaattgtc ataagaatta ataggcattt tctccactcc ttaaaattgt
    28261 ataccataca attttaccat tgattgtatt gattttagaa gttaatcttt tgtgaggagg
    28321 gaattgtcta tgaaaattat taattttgag gaagtagtag cttcttttag ttttgaagaa
    28381 gcgattcaag taatgaaaaa atgttttttt gattatgaaa gtggtaaaat cagccaaaat
    28441 cctcgattgg tggaaatttt acctgacgga aaaggcaaaa atatgtttgc gatgatgcct
    28501 gcttatttgg gggagaacag atattttggc acaaaagtca ttaccgcttt tccggataat
    28561 catcagtatc atttaccttc tcatttagga gagattttct tgtttgatgc tcaaaacgga
    28621 ctacccaaag cattgataaa cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct
    28681 gcgctggcaa cagattactt agcaaatccg catgcagaaa cacttgcttt aattggagct
    28741 ggtcaacaag cattgtcgca tttgactgcg atttctacca ttcgtcccat cactacagtg
    28801 tttgtttacg atattttaga agaaagacga aatactttta tcgctaaagc taaaaaacag
    28861 tatcctaaaa taacgtttat aaatgcaata tcagtagaag atgcagtacg tccagcggaa
    28921 atcatttgta cgttgacctc tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa
    28981 gacgcacata tcaatgctgt gggcacattt actcctgata caagagagat aagtacatcg
    29041 ttgatggaaa cttctcaaat ctatgtagat gattactcag ctgccttaac agaaagcggg
    29101 gacctgctta tagccatggc agaaggagat ttagaggaat cttgtattgt tggtacatta
    29161 ggcgaattag tttctgagaa aaaaacattt tataaaggag aatcgaggcc gacgattttc
    29221 gacgcagttg gtctagctat tgaagatctt tgttgtgcag aatatattta taacaaatta
    29281 gagaaagggg ctttaaaatg aacttagatt attacgtagt tgatgcattt gcagatgagg
    29341 tgttcaaagg aaatccagca gcggtttatg tgctagaaga atggctgcca gaaggtacga
    29401 tgcaaaaaat tgccattgaa aacaatttat ctgaaacagc atttacagtg aagaaaaacc
    29461 aagaatttga gcttagatgg tttacgccag atcgagaaat cgatctttgt ggtcacgcga
    29521 cattagcaac agcatttgtt ttatttaact attacaaaat acctgatgaa acgattaaat
    29581 tttctactca aagcggtaat ctatttgtga caagaattaa agattattat tacatggatt
    29641 ttcctagtat tatgccgaaa aaagtgccga tattgtctga atatgaagaa gcgattgggg
    29701 ctaagattaa agaagcctac ttagcacgtg atttattttt cgtattagaa gatgaaaaaa
    29761 cggtggctaa attgcagcca gattttacag ctatcaaaaa ttttgaatta ggaataggag
    29821 tcatcgtgac agcagaaagt gtgcaaaaag actttgtttc tagaaccttc tttccaaaat
    29881 taacaatcaa tgaagatcca gtatgtggat cagcacattc taatcttatt ccttattggg
    29941 cagaaagaat gaataaaaca aaattgtcag cttatcaaat gtcatcaagg ggcggggatc
    30001 tagaatgtga attaaaagaa gatcgtgtga taataggagg aaaagcagtt ttgttttcta
    30061 aaggtacctc gtttatttga tttctttcaa atgaattaaa aataaattgg tggagagtgg
    30121 caaccttata gctacaatgg ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa
    30181 gtttgcgtct cattttaatt attggtgttt gttttttatt caatcaagag taaaatgccc
    30241 catactaact atcctcggat gacttcgatt ttataaccat cagggtcaat gacaaagtag
    30301 taagaaggcg cagtgccagg taatcctttt aagtcagtaa tctcaagacc ggcagcttta
    30361 tgttgctcgt gtaatttttc tagatcatca gctgctatag cgatatgacc atagccgtct
    30421 cctagatcat acgcaccatg atcatagttg taagtcaact ctaattcata gtcatcccca
    30481 ggaagtgtca aataaacaag tgtaaatttg ttttctggaa aatcacggcg gcggctttct
    30541 tcaaaaccaa atgccttttg ataaaattcg attgatgctt caagatcttt cactcgaaca
    30601 catgtgtgag ccattttcat agattcatct ctccttttcc ttttactcaa tcataacaaa
    30661 aagaagtaag gaaatagttg atttgtgctt gttcatgaga aatttctcat gtaaaatgga
    30721 tagaaaataa aactgttaag gtggaaacta ttcatggata aaccaatttt tggtgaaaaa
    30781 aagcaatttg ctgattatca aacccgttat gcagcatata ttgtcataga aagagatcag
    30841 cagattgcag taatcgaggc tccaaatggt gctttttttc ttcctggcgg ggaaattgaa
    30901 ggagaagaga cgaaagaaga agcgattgtc cgtgagctga tagaagaaat gggaattgtg
    30961 gcagaaattt cttcttatct gggagaagct gatgagtatt tttactcgaa ttatcgtgca
    31021 acttattatt ataatccagg atatttcttt gttgctgatt cttggaagcg tataggagaa
    31081 ccaactgaaa agaccaataa aatttggtgg gtgagccctc aagaagcgct tgtgaaattg
    31141 aaacgtggta gccaccgatg ggcagtcgag agatggatcg aaaaaaataa aaactgaaag
    31201 aaagcgagta gatcagcttt ctctcagttt ttttatttat tacagtacat attttttgat
    31261 tgcttcagct acaccgtgct cgtcgtttga tgaagtgatg acatttgctg cttctttgac
    31321 aagagaaacg gcatttccca ttgctactcc tagaccagca tattcaatca tggataagtc
    31381 gttctcgtta tctccgatag ccatgatttc atcttgatca ataccaaggt gctgtgcgag
    31441 attcgcaact gccgcacctt tacttgcttc tttatttagt acttcgaagt aatagggcgc
    31501 acttttgacc gttgtgtatt tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc
    31561 taaaatttct ggttcgtcga tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc
    31621 ttcaggtgtt cgatatttta gcggcatctt gactaaactt gcttcgtgga tcgtataagc
    31681 gctgatatct cgattaggcg tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc
    31741 gacacggcgt gccatcactt cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc
    31801 gatgatttta cctgatttcg tttgttgtac gagtgccccg ttataagtaa taacgtaatc
    31861 attatcttga aaaaggttca actcgtttaa ctgttcctgg acacctggta agggacggcc
    31921 agtgcataaa acgatcgaaa caccttttgc aacagcagcg gcaatcgctt cttttacttc
    31981 ttttgttact tctcttttct catttaataa agtaccatcg atgtcgatag cgactagttt
    32041 gatagacata atttcctcca atatatttta atttatttca atcaatgcgc cattacggat
    32101 atggctggca aattcttgat aagtttcgtc aaataaatca taatgatcac gcaaggacgg
    32161 atcaaccatt tcttttggaa agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc
    32221 agcaacaagc tgactaacct gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc
    32281 gatttgtgtg cgatgtctgc cttgttctgg gcgataaaaa tcataaggta gatcgtagct
    32341 agaattcaca gcagtgtaat aaacaggatt atatccgacc ttttctacta acaaagtgag
    32401 ttcttggact aattttgaat cccggttgcc agaaaaagta gcagatttca gtggtttacg
    32461 attcaaaaaa cgtttagcta aatcatttaa aataggatca gcagattcag accattgagt
    32521 aaagtaagtg cccaagaccc catcatcaag tttgaggtat tcttctaaag taaagcttcc
    32581 gtcaagaaat ggcagaagta attgagaatg gagttcaaaa ctgctggcat cttcttgata
    32641 caactcgtgt gcgcgatgaa gcagatgctc caaaattact tccataccac gggaaacagg
    32701 atggaagtac acttgcacat acatttgata tcggctaacg atataatctt ccactgcatg
    32761 catgccactc atggaaaaag cgatcccgcc ttcataagga cgaatcacac gtaatattcg
    32821 ggtaagatca aatgttccat attctgttcc agtgaaataa gcatcccgaa gcaaatagtc
    32881 cattcgatct gcatcgatct gactagaaat catttggaca acttgtgggt tggggtaagt
    32941 tttttggatc acactagcta ccttttcagg aaaaccttct tctactcggt taagaatctg
    33001 atacacttct gtttctggag aagtgatgat ctctacagtg atggcttcat gattcgtatg
    33061 gaagatatgt tcaaatgtat gagagtaagg accatgaccg acatcatgca acagtgccgc
    33121 acataaagtc accagccgtt cctgatcatc ccagcctccg ttgcctattt tttcttttga
    33181 aaaatttcga gaaaagatat cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg
    33241 agtaaaacgg ctatgttctg ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat
    33301 gcgtcgtaga cgttggactt ctttcgaatt gatcaaatct aagattactt gatgctggac
    33361 atggatatag ttatggactg gatcacgaaa aactttttcc ataggcagca tttgatattt
    33421 ataaggggtt gtcataatgt agctccttta tgttattacg ttgttccatt ctctggattt
    33481 ggttgttcca ttgctccagc tggatttctt tttttagcca gtcggtagaa tcctgtaaat
    33541 cacttgcaga atacagcttt tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa
    33601 aaaggggttg gtcaagcagt tcttcaagtg ttgccattgt agcaggatcc actgcaggat
    33661 agccatttgt accaaagtct tcttttaaag cagattgata aaatcgcttc atgacttgcc
    33721 cgcgtttttc ctgattaccg ttgacactta agtacatcat aacagaaatc ccatttttga
    33781 tccgacgctg agcgataccg gcaaattttt tgccatggat gcttaaatca taagttccag
    33841 gacaataaga atgggtgatt tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag
    33901 tcaataggag catttgctcg taagcgtcat ctatggttat tttttttgtg tcactttggg
    33961 ggaaaatcaa agaaatattc aaaattcctt catctgatac gatccctaat ccacctgaat
    34021 ttctgatcac tggagcgtaa cctgcatctt tcagtgtttg gatcccgtca cttagaaaag
    34081 gagttcgaac atccttcata cctaagatga ctgtgcagtc atattgccag aaatgtaaaa
    34141 agatttgctg gtgttcttta gaatattccg ttaatatatc tgttaatgca aaaggggaaa
    34201 agtcgttttt ttgataaatg tgctgatcta gtcgaatcgc agggttcatt caaccggctc
    34261 ctttgtataa attcatattt tctacaatat tatactctaa aatcaaagag ttgttttgga
    34321 aaaaaggttt tctgtaaaaa tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa
    34381 tgtagctatg agaagtaaag gagaatgaat aatggaatta tcacaaggaa caccgatttc
    34441 aatcaaattg cgtacaaaag taaaacagca aggggaagtg caagattttt attttgattt
    34501 gaaagggcaa atggtgacaa ttggcgatac gctgtacata cgttataaag agattcagga
    34561 ggagacgggc gatgagattc ctgtaacgat caaattagtt ccagatggtc atgtacagct
    34621 gatccgtgca ggagaaatgc ggatgcgttt gaaatttggc tataaagaac ggttagaaac
    34681 atcctatcga acaccatacg gtatgatcca gattgctact tttacgaaag aactgcatgt
    34741 tagtttgaaa gaccgtccca caggagggaa ggttcgaatc gattatgatc tgttcatggg
    34801 accggaaaaa atcggcgaat attatttgac cttggatttt acagcgtaaa tataaaagaa
    34861 aagtttgatt ttttgtgtct gattttgtat gataaggagt agactgtgaa aggacgtgtc
    34921 ctgattggaa attagtgtat ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt
    34981 agcacacgcg atcttagaac aacgtggcga tgtcatggat ttttctgatt tagctaacca
    35041 gatccaaaac tatcttggga aatcagacag tgaaattcgt gattcactcg cacaatttta
    35101 tactgacttg aatattgacg gcagctttat ctcattaggg gataatcgtt ggggcttgcg
    35161 ttcatggtat gctatcgatt cgatcgatga agaagtaaac catggaatgg acgaagagga
    35221 tgaagatact ccacgtcgcc gtaaacgcaa gaaagtcaac gcctttatca atgatgatga
    35281 agacgcgatc gattacaatg acgatgaccc agaagataca gatctgacag aagaagatga
    35341 cgatgatctg tttgatgacg atgatgatga agatgaagaa atcgctgctt ataattctga
    35401 cttgcaagaa atcggagcag atgacccgac agatgatgaa gaagacttgc caggaaatat
    35461 cgaagaagat ttaagtatca tagacgaaga cgatgatgat gaagattatg aagaggaaga
    35521 attttctgac tctgaaaaag aataaacatt attttcttac gagaggttgt gacagagacg
    35581 atcagcctat cagaataagg aggtgtgaca cgatttttgt cacaccttct tttttgtata
    35641 taaaaatccc aagtaaatac ataaaaaata aaaaaattga aacttttagc gcaccttgta
    35701 cgtcatatta agtgaggcag aaaggagtgg agaatgattt ttactggata taagaaaaaa
    35761 cggcagatcg aaaaataccg tcacctgctg aatggactca tcgaaactga ttatcaaaaa
    35821 atgttccgta tcacgatgaa ttacgtacat gataaagaag aggccttaga tgtgatgcaa
    35881 gatagttttc ataaagccct gaactatttt gaaaaagaaa aagagatcga gcattttagt
    35941 gcatggtttt atcggatatt gatccgcacg gcattagata gttggcgcag acaaaaaagg
    36001 acgcctgtgg acttatttga aattcaagat atcccccaac agttaccttt agaaacttcc
    36061 gttgcagagc tccatatgat tttagataaa atagacagtc cggaaaaaga gattctgatc
    36121 ctctactttt ttgaaggatt tcgtttgaaa gaaattgcga ctatcttgga tatgaatgaa
    36181 aacacagtta aaacgaaaat gtaccgctca ttgaatgctt tacgtcaaat cctagaatga
    36241 aggagagaag agtgccatgt attcccaaaa agaaaattta aaaaaactaa agaaaactta
    36301 taaagaacaa gcaattccca aagggcttaa agaggagatc cgccagcgtt ttattatcga
    36361 agagcaatca tttataaaag caaagcggag aaagagacgt ctccaagggt tcggagtatc
    36421 tttggctatc gtaatgatta caacaagtag ccttcttttt aatagtcaag ttcgaagctt
    36481 tgcagaagat ttacccatct taggatcagt cattgaattg atcttaggcg aacgctttac
    36541 tgatcgatct gaaaaaatcg atattcaggt accgaagatc aatacacaaa atgagaaaga
    36601 aaataaaaca attcatggat taaatcaaaa atactttcgc gaaggacaag cagattttga
    36661 aaaagctgaa aaagaatatg gaaattttga aacggatcat taccaagttt tgggagatta
    36721 tcagaaaatc gtagacgata accggtttct cgtgattgaa aggcagatca cccaaacagc
    36781 agcagatagc catgttgaaa aaagatacga cacaatcgac aagaaaaaca gtgtccagct
    36841 ctcacttcca cttttattca aagatgacac ctatctatct gttttgacaa aagaagtcaa
    36901 acggcagatg gccgaacaag tcaaggaaga tccatctaag tactattgga ctgagcaaga
    36961 tattcaagaa gggacgatag aaaaaccaac acttgttacg cctacacgaa gtttttattt
    37021 gaacaaagag catcaattag ttttgacttt ttctcaatac gaaattgctc ctggttatat
    37081 gggtacacca gaatttgtca ttcctaagtc cgtgacaaaa acgattttgg catcggaaga
    37141 ttatttggat cattagacga gaagtagttg cgactagcag tttcaatttt caagtaagta
    37201 caagtaaata ataagaagag aaaaagagaa agaagagata tagtgaaaag aaattattta
    37261 gttatcagta ttattgcttt atccgtgctt ttgtttttcg aaattacttc ggtggcctcc
    37321 ttgatcaccg aagtaaaagg acaaacacac tcttcggagc attcgtcaca atctttcaaa
    37381 caatcagaac aatcaaccat taaaagccaa tcaactgaaa cagctaacgt caaagaagcc
    37441 attcaagaat cagcggaaaa ggtacaaagt cagtcatcac aatcaacaca agacagtaca
    37501 aaaacgagcc gagatccggc tgtcaaaagc gtcgcaatca gctttgatga tggaccagga
    37561 gcgaccacaa caccgcagct tctgcgaatc ttaaaagaaa aaaacgtgca tgccactttt
    37621 ttcgtcttag gagaaaatac ggcgcagcat cctgaaatcg tgaagcagac agctgaagca
    37681 gggcacgaga ttggcaatta cacatacgat catcagaatc tggccactct ttctgcccag
    37741 tcgatgacgg aggaagtgac gaaagcagac actgagatca aaaaagtcac tggcaaaacc
    37801 cccacttttg ttcgtccgcc ctatggttct gtcacgaatg taggagcaac gatcatccag
    37861 cgaccgatca tcgaatggtc tgttgattca gaagattgga agacaagaaa tcctgatttg
    37921 attttgcaga agatccaagc aacagtatac gatggggcaa ttattttatt ccatgatatc
    37981 tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga gcaagggtat
    38041 cgcatcacga cagttgggga tttattaggg catccaacgg ctgtcgagaa ctattatggc
    38101 agaaacgatc atcggccagt tcagtaagtg agacaagtat ctttaataaa taacacattt
    38161 taataagtag ttgaacacat aactacctta cttgcaaagg cttaacaata tctatacagg
    38221 tacaacaaaa aactgtccac caattttctt tggcggacag ttttcactta aaaataaaga
    38281 agcaactatt cgtaaattat gctaaaatag cagtagaacg cgtcctgagg gaatcgaacc
    38341 cccgtctcaa gaaccggaat cttacgtgat atccactaca ctaaggacgc aagcactggg
    38401 ataattgtaa ctaaacagat aaaaaattac aaggggattg gacaaaaaaa tgaaatttga
    38461 gcaaaatttt tcacaaaaac agcagcagac acagaaacta gccatgacgc aacagttgca
    38521 acagtccatc caagttttac aatataacag tgaggagctg ttggcattcg ttgagaatca
    38581 agcaatggaa aatccattgg tggaagttgt tgagccggaa tggcagccag attacataaa
    38641 agcttcttca tcttcttatg aaggagaaga gacaaattac ctaaatcaga ttcctgatac
    38701 aaaagggtct ttatttgatt cattgatcga acaagtccat ttgaattacc gagatacttt
    38761 tttgaggaaa atcgttcttt atcttgtaga atacatcgat ttgaacggat ttttgacgat
    38821 tagtttagaa gaagccggaa aagagattgg agctacgcct atccaaatgc tcgatgcgtt
    38881 gactctgatt cagcaattag aaccagctgg agtaggtgca cgtagtttgc aagaatgtct
    38941 catgcttcaa acagaacgag atgattatgc gccggaatta gcttatatcg tgttggaaga
    39001 atgttttgag gaactggtgg agcgaaaatg gaaagaaatc gcacaacgtt tttctgttga
    39061 tctacatgct gttcagcaga tattcgacta tttacaaacg ttgagtccaa gccctggccg
    39121 gattttcgac cgtagcagcg aattgtacat tattcctgat gtccgggtat tagtggacga
    39181 cgataaaaat gtacaagtta tctcaaaccg aaaaaatcag ccaaatatac gctttcaaga
    39241 gagttacttc aaacaaatgt cacaacaagc agataaagaa actgaaaatt atttaaaaga
    39301 acggaaacag gaattcgaat ggttgaagaa aacgatccta caacgtgggg atacaatttt
    39361 gcgggttgct caagtcattg tttcccggca aaaagctttc tttatcgaca aggaacgtcc
    39421 aatcaagcct cttacgttaa aggaagttgc aactgagata gatgtgcatg aatccacggt
    39481 cagtcgtgca gtcaacggga aatatttgga aacagatttc ggtgtatttg aattaaaaaa
    39541 atttttcact acacgaattc ctacgaatag tacagaacag acagaagacc tttcagcaga
    39601 tacagcgaag aaaaagctgc aggaattagt cgatcaagaa gacaaaaaca agccgttgtc
    39661 ggaccagaaa ttggttgaat tgctgaaaaa agatgagata gcgatctcaa gacgaacagt
    39721 agcaaaatat cgtgatttat taggaatccc gagttcatca aaaagaaaac gttatgataa
    39781 ctaatcagtc aaaagactag aaaaagtatt tcactttttc tagtcttttt agttgaaaaa
    39841 cgagagcgaa actgttagac ttacagtcgt gaggttgaga tttcccttta ttgatgtgaa
    39901 attcgaatag ttaatttctt tttttatcgt atatgggtcg ttttaagtct acattggacg
    39961 taaagtgtcc aactaaggag atcgctatgc tggatgaaat aaaaatgatt gaatctgttg
    40021 caccagatat tatcgaagtg ttgcaagaac gctataaaat tttacgaaat atctattgga
    40081 tgcagccgat tgggcgtcga agtttatcgg aaagtatggg gatcacagaa cgagtcttac
    40141 gaacagaaac agaccttttg aaaaatctga atttgatcga cacctcaaaa agcggtatga
    40201 cgctgacaac taagggcgaa gaagtttatc aaagccttga aaacttcatg gatcagcttt
    40261 taggtacgca tcaaaccgaa caacagttgg cagaatattt tggtaaccaa cgctgtatcg
    40321 ttgtttcagg aaacagcgaa gagcagacca aagtggctga tgcattcggc gaagcgctga
    40381 gcgaagcctt agaccgacta cttccagaag gcgagaatat catcgctgtg atgggaggaa
    40441 ctactatggc tgtggtagcc gaacaattgt ctaatctgga aaataagaaa cggcataacc
    40501 tgtttgtccc tgcaagaggc gggattggtg aagcgataac tgtacaggca aattctgtca
    40561 gtgcaagaat ggctgctaaa gcaaacggta accaccgcgc actttatgtt ccggaacaat
    40621 taagtctggc aacttacaat tctttactca atgagccatc tatccaagaa gtcttaaact
    40681 tgattagtga agcgaattgt gttattcata gtattgggcg tgcattacac atggcagcac
    40741 gtcgtaagat gactgaaaaa gaattagtca tgctgaaaca agcaaatgct gttgcggaat
    40801 cttttggcta ctttttcaat gaaaaaggtg aagtcgttta taaggtccct agaatcggca
    40861 tagaattgca agatttggaa aaagtcccga tcatcatggc aatcgcgggc ggaaaatcaa
    40921 aagctaaagc aattcgagct tatatgaaaa atgcaccaaa acaaacgtgg ctcatcactg
    40981 atgaagctgc tgcaaatgag attttaaaag gggtaaccct ttaaaataaa tatttttttg
    41041 attttcataa ggaggaaatc ttaaatgaca gttaaagtag gtattaatgg ttttggacgt
    41101 attggacgtc tagcattccg tcgtatccaa gatgtagatg gaatcgaagt agtagcaatc
    41161 aacgacttga cagatgctaa aatgttggca cacttgttaa aatacgacac aactcaagga
    41221 cgtttcaacg gaacagttga agttcatgaa ggttcattca acgtaaacgg aaaagaagtt
    41281 aaagtattag ctaaccgcaa cccagaagaa ttaccatggg gcgaactagg cgtagatatc
    41341 gttctagaat gtactggttt cttcacttct aaatcagcag ctgaaaaaca tttaacagct
    41401 ggtgctaaac gcgttgttat ctctgctcct ggcggaaacg atgttccaac aatcgtttat
    41461 aacacaaacc acgaaacatt aactggtaaa gaaacagtta tctcaggtgc ttcatgtact
    41521 actaactgtt tagctcctat ggctaaaaca ttgaacgaca aatttggtgt tgttgaagga
    41581 ttaatgacaa ctatccacgc ttacacaggt gaccaaatga ctctagacgg acctcatcct
    41641 aaaggtgact tccgccgtgc acgcgctgct gctgcaaaca tcgttcctaa ctcaacaggt
    41701 gctgctaaag ctatcggttt agtaattccc gaattgaacg gtaaattaga cggagctgct
    41761 caacgtgttc ctgtaccaac aggttcatta acagaattag taacagttct tgaaaaagaa
    41821 gtaactgttg acgaaatcaa tgcagcaatg aaagaagctt caaacgaatc ttacggatac
    41881 aacacagacg aaatcgtttc ttcagatatc gttggtatga cttacggttc attatttgat
    41941 gctacacaaa ctaaagtaat gacagttggc gacaaacaat tagttaaaac tgttgcttgg
    42001 tacgacaacg aaatgtcata cactgcacaa ttagtacgta ctttagaata cttcgctaac
    42061 ttataagata gacaaacgta caagtgtaaa ttatagacat gtacatttaa aagcggggaa
    42121 gcaatcgcgc ttcttccgct ttttttgata ataagtttat tcaggaggaa ataaaatggc
    42181 taagaaaaca gtgaaagata tcgatttaaa agacaaaaaa gttcttgtcc gtgttgactt
    42241 caacgttcct ttgaaagacg gcgtgatcac tgacgacact cgtatcaaag cagcattgcc
    42301 aacaatcaac tacgttctag aacaaggcgg aaaagcaatc cttttctctc accttggacg
    42361 tgtaaaaaca gaagaagata aagaaggaaa atctttagct ccagttgcaa aacgtctagg
    42421 cgaattatta ggtaaagaag taacattcgt acctgaaact cgcggagaac aattagaaga
    42481 agcaatccgc aacatgaatg acggcgacgt tgtcgtattc gaaaatacac gatttgaaga
    42541 tattgacggt aaaaaagaaa gcaaaaatga tacagaacta ggtaaatact gggcttcatt
    42601 aggcgatgta tttgttaacg atgctttcgg tacagctcac cgtgcacatg cttctaacgt
    42661 aggtatcgct tcaactggta tcccaacagt tgctggattc ttgatggaaa aagaaatcaa
    42721 gttcatcggt gaagcagtag aagaaccaaa acgaccaatg attgcaatcc ttggtggcgc
    42781 aaaagtttct gataaaatcg gcgtaatcga aaacttgatt cctaaagcag ataaaatctt
    42841 gatcggtggc ggaatgactt acacattcta cgaagcaaaa ggaatcaaga tcggtaactc
    42901 tctagtagaa gctgataaag tggaactagc aaaagaatta atcgaaaaag caggcgacaa
    42961 acttgtcttg ccaatcgaca atgtctgtgc accagaattt tcaaatgatg ttgaaacaca
    43021 agtaatcgaa ggagacattc ctgatggttt gatggcttta gacatcggcc cagcatcagt
    43081 aaaattattt gctgatacat tacaaggtgc aaaaacagtt gtttggaacg gacctatggg
    43141 tgtatttgaa atgagtaact ttgctaaagg tacaatcggt gtttgtgaag caatcgctaa
    43201 cttagaaggc gcaacaacaa tcatcggtgg cggagactca gccgcagcag cagaacaatt
    43261 aggatttgct gacaaattca cacacatctc aacaggtggc ggcgcaagct tggaattgct
    43321 tgaaggtaaa gaattaccag gactagcagc aatcaacgat aaataaacga ccaagtttgc
    43381 tcaattctga cgagtaatcg aacaaaaggt gaagggagtt ttcatgactc cctagccatt
    43441 tttcaataaa ttaaggagtg tgttcctcaa tgcgtaaacc tattatcgca ggtaactgga
    43501 aaatgaacaa aactgcatca gaagcaaaag aatttgctga agcagtaaaa aacaaaatcc
    43561 cttcaaatga caaagttgat tcagtcatcg gatctcctgc attattctta caagaattaa
    43621 tgacatcagc tgaaggtaca gaactaaaaa tctcagcaca aaactgctac tgggaaaact
    43681 caggtgcgtt cactggtgaa acttcaccag cagctcttgc tgatctaggt gttcattatg
    43741 tgatcatcgg acattcagaa cgtcgtgaat atttccatga aacagacgaa gacatcaata
    43801 aaaaagcgaa agctatcttt gcaaatggta tgacaccaat cctatgctgc ggcgaatctt
    43861 tagaaaccta tgaagcaggc aaaactgctg aatggatcga aggacaaatt acagctggtt
    43921 tagctgatct ttcagctgaa caggtgtcaa acatggttat tgcttatgaa ccaatctggg
    43981 caatcggtac tggtaaatca gctgacgcac aaatcgctga cgaaatttgt ggagttgtac
    44041 gtcaaacagt tgaaaaactt tatggaaaag aagtatcaga agcagttcgt atccaatacg
    44101 gcggttctgt aaaaccagaa aatattgctg aatacatggc aaaagaaaat gttgacggtg
    44161 ctttagtcgg tggcgcaagc ttgcaagcag attcattctt gtcattgtta gacgctgtaa
    44221 aataattgtt gaaaatttgc gaaaaatgca agaaatgttt gcactaatga gcaaaattaa
    44281 ctaaaatcgt atatgaggga aatcccttag gaaaaaacaa actcaaagga gagacaaaac
    44341 atgtcaatta ttactgatgt ttacgctcgc gagatcttag actcacgtgg taacccaaca
    44401 atcgaagtag aagtatacac agaaagcgga gcttttggcc gcggaatggt tccttcaggt
    44461 gcttcaactg gtgaatacga agcagttgaa ttacgtgacg gagacaaagc tcgttatggc
    44521 ggtaaaggtg taactaaagc tgtcgacaac gtaaacaaca tcattgctga agcaatcatt
    44581 ggctacgatg ttcgtgacca aatggctatc gacaaagcta tgatcgcttt agacggaact
    44641 cctaacaaag gtaaattagg tgcgaacgct attttaggtg tttctatcgc tgttgcacgt
    44701 gcagctgctg actacctaga agtaccttta taccactacc taggcggatt caacacaaaa
    44761 gtattgccaa caccaatgat gaacatcatc aacggcggat cacatgctga taactcaatc
    44821 gacttccaag aattcatgat catgcctgtt ggcgctccaa cattcaaaga agctctacgt
    44881 atgggtgctg aagtattcca cgcattagct tcaatcttga aagctcgcgg attagcaact
    44941 tctgttggtg acgaaggtgg atttgctcca aaccttggtt caaacgaaga aggttttgaa
    45001 gtaatcatcg aagcaatcga aaaagctggc tatgtacctg gtaaagacgt tgttcttgct
    45061 atggatgctg cttcttcaga attctacgac aaagaaaaag gtgtttacgt actagctgac
    45121 tcaggcgaag gcgaaaaaac aactgacgaa atgatcaaat tctacgaaga attagtttct
    45181 aaatatccaa tcatctcaat cgaagatgga ttagacgaaa acgactggga cggattcaag
    45241 aaattaactg acgtattagg cgacaaagtt caattagttg gtgacgactt gttcgtaaca
    45301 aacactcaaa aattatctga aggtatcgaa aaaggaattg ctaactcaat ccttatcaaa
    45361 gtgaaccaaa tcggtacatt aacagaaact ttcgaagcta tcgaaatggc taaagaagct
    45421 ggctacacag cagttgtatc tcaccgttct ggtgaaacag aagattcaac aatctctgat
    45481 atcgctgttg caacaaacgc tggccaaatc aaaactggtt ctctatcacg tactgaccgt
    45541 attgctaaat acaaccaatt actacgtatc gaagaccaac ttggtgaagt tgcagaatac
    45601 aaaggtttga aatctttcta caacttaaaa aacaaataat tcttttctaa tgaagataca
    45661 tgatcgacta ttgaaagata gtcgagtatg atcacttagt taagtaatcg aactctctac
    45721 ttgctgaaat atcagcaggt agagagtttt tttgcaacta gcttctgtat atgatgttta
    45781 caaagaatgg tacaagaaaa gatcatcctt gaaaaaggaa agctaacaat aactagcatc
    45841 atataagagc ttttaaggat agaacagtag aataggaatc tatgggcaag tcctctcgta
    45901 taaataaaat aggagggaag tagcagcata tttttcataa agtggctctc tttcttcatt
    45961 tttactttgg ttgctcctat ttgaaaattc gagcacactc aaatacaata aaaagaaatg
    46021 gcaagggaga ggattagatg aaaaaagaag aaaaaatcgc gattttacaa gagattatcc
    46081 ga
//
LOCUS       DAPCMU010000005        43646 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPCMU010000005 DAPCMU010000000
VERSION     DAPCMU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.005"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="RD744_000234"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="RD744_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDU0556268.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="RD744_000235"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="RD744_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDU0556269.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="RD744_000236"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="RD744_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDU0556270.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="RD744_000237"
     CDS             1642..2274
                     /locus_tag="RD744_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDU0556271.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="RD744_000238"
     CDS             complement(2276..3181)
                     /locus_tag="RD744_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDU0556272.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="RD744_000239"
     CDS             complement(3266..3925)
                     /locus_tag="RD744_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDU0556273.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="RD744_000240"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="RD744_000240"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDU0556274.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="RD744_000241"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="RD744_000241"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDU0556275.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="RD744_000242"
     CDS             6168..7253
                     /locus_tag="RD744_000242"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDU0556276.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="RD744_000243"
     CDS             7250..8314
                     /locus_tag="RD744_000243"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDU0556277.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="RD744_000244"
     CDS             complement(8563..9366)
                     /locus_tag="RD744_000244"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDU0556278.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="RD744_000245"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="RD744_000245"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDU0556279.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="RD744_000246"
     CDS             complement(10244..11251)
                     /locus_tag="RD744_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDU0556280.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="RD744_000247"
     CDS             complement(11258..11902)
                     /locus_tag="RD744_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDU0556281.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="RD744_000248"
     CDS             complement(12002..12760)
                     /locus_tag="RD744_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDU0556282.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="RD744_000249"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="RD744_000249"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDU0556283.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="RD744_000250"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="RD744_000250"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDU0556284.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="RD744_000251"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="RD744_000251"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDU0556285.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="RD744_000252"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="RD744_000252"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDU0556286.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="RD744_000253"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="RD744_000253"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDU0556287.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="RD744_000254"
     CDS             18158..19027
                     /locus_tag="RD744_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDU0556288.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="RD744_000255"
     CDS             complement(19153..20052)
                     /locus_tag="RD744_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556289.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="RD744_000256"
     CDS             complement(20217..21041)
                     /locus_tag="RD744_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDU0556290.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="RD744_000257"
     CDS             complement(21052..22347)
                     /locus_tag="RD744_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDU0556291.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="RD744_000258"
     CDS             complement(22363..22842)
                     /locus_tag="RD744_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDU0556292.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="RD744_000259"
     CDS             complement(22848..23876)
                     /locus_tag="RD744_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDU0556293.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="RD744_000260"
     CDS             complement(23893..24999)
                     /locus_tag="RD744_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDU0556294.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="RD744_000261"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="RD744_000261"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDU0556295.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="RD744_000262"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="RD744_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDU0556296.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="RD744_000263"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="RD744_000263"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDU0556297.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="RD744_000264"
     CDS             29970..30668
                     /locus_tag="RD744_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDU0556298.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="RD744_000265"
     CDS             complement(30836..33475)
                     /locus_tag="RD744_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0556299.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="RD744_000266"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="RD744_000266"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDU0556300.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="RD744_000267"
     CDS             35318..36493
                     /locus_tag="RD744_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0556301.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="RD744_000268"
     CDS             36497..37138
                     /locus_tag="RD744_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556302.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="RD744_000269"
     CDS             37135..38052
                     /locus_tag="RD744_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0556303.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="RD744_000270"
     CDS             38049..38720
                     /locus_tag="RD744_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556304.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="RD744_000271"
     CDS             complement(39261..39749)
                     /locus_tag="RD744_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDU0556305.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="RD744_000272"
     CDS             complement(39950..40216)
                     /locus_tag="RD744_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDU0556306.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="RD744_000273"
     CDS             complement(40216..40479)
                     /locus_tag="RD744_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0556307.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="RD744_000274"
     CDS             40955..41140
                     /locus_tag="RD744_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0556308.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="RD744_000275"
     CDS             complement(41282..43105)
                     /locus_tag="RD744_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDU0556309.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT        12464 a         9468 c         7978 g        13736 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tttatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatt
//
LOCUS       DAPCMU010000006        43086 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPCMU010000006 DAPCMU010000000
VERSION     DAPCMU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.006"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            212..604
                     /locus_tag="RD744_000276"
     CDS             212..604
                     /locus_tag="RD744_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0556310.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="RD744_000277"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="RD744_000277"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDU0556311.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="RD744_000278"
     CDS             3165..4775
                     /locus_tag="RD744_000278"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDU0556312.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="RD744_000279"
     CDS             5067..5936
                     /locus_tag="RD744_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDU0556313.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="RD744_000280"
     CDS             6101..7372
                     /locus_tag="RD744_000280"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDU0556314.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="RD744_000281"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="RD744_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDU0556315.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="RD744_000282"
     CDS             8763..9026
                     /locus_tag="RD744_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDU0556316.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="RD744_000283"
     CDS             9471..9920
                     /locus_tag="RD744_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0556317.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="RD744_000284"
     CDS             9977..11395
                     /locus_tag="RD744_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0556318.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIVIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="RD744_000285"
     CDS             11395..12255
                     /locus_tag="RD744_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDU0556319.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="RD744_000286"
     CDS             12429..13202
                     /locus_tag="RD744_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0556320.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="RD744_000287"
     CDS             13349..13714
                     /locus_tag="RD744_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDU0556321.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="RD744_000288"
     CDS             complement(13862..15070)
                     /locus_tag="RD744_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0556322.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="RD744_000289"
     CDS             complement(15089..15367)
                     /locus_tag="RD744_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556323.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="RD744_000290"
     CDS             15521..16216
                     /locus_tag="RD744_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDU0556324.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="RD744_000291"
     CDS             complement(16658..17455)
                     /locus_tag="RD744_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDU0556325.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="RD744_000292"
     CDS             complement(17452..18636)
                     /locus_tag="RD744_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDU0556326.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="RD744_000293"
     CDS             complement(18661..19908)
                     /locus_tag="RD744_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDU0556327.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="RD744_000294"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="RD744_000294"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDU0556328.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="RD744_000295"
     CDS             21888..22202
                     /locus_tag="RD744_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDU0556329.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="RD744_000296"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="RD744_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDU0556330.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="RD744_000297"
     CDS             22887..23531
                     /locus_tag="RD744_000297"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDU0556331.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="RD744_000298"
     CDS             23546..23875
                     /locus_tag="RD744_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDU0556332.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="RD744_000299"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="RD744_000299"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDU0556333.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="RD744_000300"
     CDS             24863..25687
                     /locus_tag="RD744_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDU0556334.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="RD744_000301"
     CDS             25680..26027
                     /locus_tag="RD744_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDU0556335.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="RD744_000302"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="RD744_000302"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDU0556336.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="RD744_000303"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="RD744_000303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDU0556337.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD744_000304"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD744_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDU0556338.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD744_000305"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD744_000305"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDU0556339.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="RD744_000306"
     CDS             31657..32451
                     /locus_tag="RD744_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556340.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="RD744_000307"
     CDS             32441..34483
                     /locus_tag="RD744_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556341.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="RD744_000308"
     CDS             34764..34913
                     /locus_tag="RD744_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDU0556342.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="RD744_000309"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="RD744_000309"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDU0556343.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="RD744_000310"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="RD744_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDU0556344.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="RD744_000311"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="RD744_000311"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDU0556345.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="RD744_000312"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="RD744_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDU0556346.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="RD744_000313"
     CDS             39330..40412
                     /locus_tag="RD744_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDU0556347.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="RD744_000314"
     CDS             40412..41215
                     /locus_tag="RD744_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDU0556348.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="RD744_000315"
     CDS             41285..42025
                     /locus_tag="RD744_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDU0556349.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="RD744_000316"
     CDS             42036..43040
                     /locus_tag="RD744_000316"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDU0556350.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13854 a         7527 c         9006 g        12699 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgtgatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 tttttt
//
LOCUS       DAPCMU010000007        42094 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPCMU010000007 DAPCMU010000000
VERSION     DAPCMU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.007"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            151..798
                     /locus_tag="RD744_000317"
     CDS             151..798
                     /locus_tag="RD744_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDU0556351.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            813..2339
                     /locus_tag="RD744_000318"
     CDS             813..2339
                     /locus_tag="RD744_000318"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDU0556352.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="RD744_000319"
     CDS             complement(2388..5186)
                     /gene="ileS"
                     /locus_tag="RD744_000319"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDU0556353.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5448..6155)
                     /locus_tag="RD744_000320"
     CDS             complement(5448..6155)
                     /locus_tag="RD744_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDU0556354.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6176..6958)
                     /locus_tag="RD744_000321"
     CDS             complement(6176..6958)
                     /locus_tag="RD744_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDU0556355.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7007..7294)
                     /locus_tag="RD744_000322"
     CDS             complement(7007..7294)
                     /locus_tag="RD744_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDU0556356.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7309..7908)
                     /locus_tag="RD744_000323"
     CDS             complement(7309..7908)
                     /locus_tag="RD744_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDU0556357.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7922..8599)
                     /locus_tag="RD744_000324"
     CDS             complement(7922..8599)
                     /locus_tag="RD744_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDU0556358.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="RD744_000325"
     CDS             complement(8616..9857)
                     /gene="ftsZ"
                     /locus_tag="RD744_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDU0556359.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="RD744_000326"
     CDS             complement(9880..11205)
                     /gene="ftsA"
                     /locus_tag="RD744_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDU0556360.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11361..12581)
                     /locus_tag="RD744_000327"
     CDS             complement(11361..12581)
                     /locus_tag="RD744_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDU0556361.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12596..13684)
                     /gene="murG"
                     /locus_tag="RD744_000328"
     CDS             complement(12596..13684)
                     /gene="murG"
                     /locus_tag="RD744_000328"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDU0556362.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13710..15071)
                     /locus_tag="RD744_000329"
     CDS             complement(13710..15071)
                     /locus_tag="RD744_000329"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDU0556363.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15085..16047)
                     /locus_tag="RD744_000330"
     CDS             complement(15085..16047)
                     /locus_tag="RD744_000330"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDU0556364.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16073..18265)
                     /locus_tag="RD744_000331"
     CDS             complement(16073..18265)
                     /locus_tag="RD744_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDU0556365.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="RD744_000332"
     CDS             complement(18266..18667)
                     /gene="ftsL"
                     /locus_tag="RD744_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDU0556366.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="RD744_000333"
     CDS             complement(18672..19631)
                     /gene="rsmH"
                     /locus_tag="RD744_000333"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDU0556367.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="RD744_000334"
     CDS             complement(19652..20083)
                     /gene="mraZ"
                     /locus_tag="RD744_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDU0556368.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20253..20621)
                     /locus_tag="RD744_000335"
     CDS             complement(20253..20621)
                     /locus_tag="RD744_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDU0556369.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20792..21748
                     /locus_tag="RD744_000336"
     CDS             20792..21748
                     /locus_tag="RD744_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDU0556370.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21821..21937
                     /locus_tag="RD744_000337"
     CDS             21821..21937
                     /locus_tag="RD744_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDU0556371.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21992..23674)
                     /gene="recN"
                     /locus_tag="RD744_000338"
     CDS             complement(21992..23674)
                     /gene="recN"
                     /locus_tag="RD744_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDU0556372.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23692..24141)
                     /locus_tag="RD744_000339"
     CDS             complement(23692..24141)
                     /locus_tag="RD744_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDU0556373.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24248..25066)
                     /locus_tag="RD744_000340"
     CDS             complement(24248..25066)
                     /locus_tag="RD744_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDU0556374.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25075..25965)
                     /locus_tag="RD744_000341"
     CDS             complement(25075..25965)
                     /locus_tag="RD744_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDU0556375.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25967..26191)
                     /locus_tag="RD744_000342"
     CDS             complement(25967..26191)
                     /locus_tag="RD744_000342"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDU0556376.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26196..27533)
                     /locus_tag="RD744_000343"
     CDS             complement(26196..27533)
                     /locus_tag="RD744_000343"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDU0556377.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27534..28388)
                     /locus_tag="RD744_000344"
     CDS             complement(27534..28388)
                     /locus_tag="RD744_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDU0556378.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="RD744_000345"
     CDS             complement(28485..28934)
                     /gene="nusB"
                     /locus_tag="RD744_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDU0556379.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28927..29352)
                     /locus_tag="RD744_000346"
     CDS             complement(28927..29352)
                     /locus_tag="RD744_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0556380.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29598..30662)
                     /locus_tag="RD744_000347"
     CDS             complement(29598..30662)
                     /locus_tag="RD744_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDU0556381.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30834..31079)
                     /locus_tag="RD744_000348"
     CDS             complement(30834..31079)
                     /locus_tag="RD744_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0556382.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31218..31592)
                     /locus_tag="RD744_000349"
     CDS             complement(31218..31592)
                     /locus_tag="RD744_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDU0556383.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31675..32589)
                     /locus_tag="RD744_000350"
     CDS             complement(31675..32589)
                     /locus_tag="RD744_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDU0556384.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32610..33413)
                     /locus_tag="RD744_000351"
     CDS             complement(32610..33413)
                     /locus_tag="RD744_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDU0556385.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33446..34435)
                     /gene="manX"
                     /locus_tag="RD744_000352"
     CDS             complement(33446..34435)
                     /gene="manX"
                     /locus_tag="RD744_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDU0556386.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34538..35029)
                     /locus_tag="RD744_000353"
     CDS             complement(34538..35029)
                     /locus_tag="RD744_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0556387.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35220..38027)
                     /locus_tag="RD744_000354"
     CDS             complement(35220..38027)
                     /locus_tag="RD744_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0556388.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38461..38754)
                     /gene="rpmA"
                     /locus_tag="RD744_000355"
     CDS             complement(38461..38754)
                     /gene="rpmA"
                     /locus_tag="RD744_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDU0556389.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38767..39111)
                     /locus_tag="RD744_000356"
     CDS             complement(38767..39111)
                     /locus_tag="RD744_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDU0556390.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39138..39446)
                     /gene="rplU"
                     /locus_tag="RD744_000357"
     CDS             complement(39138..39446)
                     /gene="rplU"
                     /locus_tag="RD744_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDU0556391.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39466..39542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39728..40369
                     /locus_tag="RD744_000358"
     CDS             39728..40369
                     /locus_tag="RD744_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDU0556392.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40431..42062)
                     /locus_tag="RD744_000359"
     CDS             complement(40431..42062)
                     /locus_tag="RD744_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDU0556393.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT        11860 a         9168 c         7155 g        13911 t
ORIGIN      
        1 acgaaagaat gagtcttctc tatcatcttt cgttttttgt tgtaaaaacg tctaaaaatc
       61 ctttgttttt tatttagaaa aatagtcaat taacgttttt tttgttacac ttactaatga
      121 tggtttttta ccatgaaagg atgatcgaat atgacaccaa atcgagaaga ttatttgaaa
      181 atcattttag aactaggtgg agatacaacg aaagtcaaca ataaacaaat cgtatctagt
      241 ctagatgtct cagctgcatc tgtcagtgaa atgatttcta aacttgtgaa agaagagctt
      301 gtagaacatt ccccttatca aggtgtacaa ttgactgcaa gcggactgca aaaagctagc
      361 agtctgattc gcaaacatcg cttatgggaa gtttttttag ttgaacattt agactatagc
      421 tggaatgaag tccatgatga tgctgaagta ttggaacatg tgacttcgga acatcttgca
      481 gatcatttag aaaactattt gaatcatcct gaacattgtc ctcatggagg atcgattcct
      541 aaaaaagagg aagttgtcca tgaagaacgc cgacaaacat tggagtccta tcctgtcggt
      601 acaaaagtgc gtatcgcacg agtgcttgat gaaaaagaat tgttggatta cttagtcact
      661 attgacctga atatcaacga agaatatgag atcgttgaca tcgcagctta tgaaggaccg
      721 atcactatcc aaaatgatag aaagaaaatc cccgtcagct ttaaggcggc cagcacgatt
      781 tttgtcgata aaatataaag gagtgtatcc acatgtctaa tgaaaaaaat gtattgttta
      841 cgatattcgg cggtacaggt gacctagccc agcgtaaatt gtacccgtct ttgttccgcc
      901 tttacagaaa aggaaattta ggcgagcatt ttgctgtaat cggaaccgct cgccgtccct
      961 ggagcgatga gcactaccgt gaagttgtca aagaaacaat ccgtgcattg aatccttcag
     1021 atgaagaagc aactaccttt gcaagccatt tttactatca atcccatgat gtaaatgatt
     1081 caacgcatta tcagacatta aaagaattgt ctgaccgatt agatggacac tatcaattaa
     1141 acggtaaccg tgtctattat ttggctatgg cacctcaatt ttttggaacg atcgtttctc
     1201 atttgaaatc acaaaatatt atgacaaaaa atggcttcaa tcgtttgatc atagaaaaac
     1261 cgttcggttc tgattaccag tctgcttttg aattgaacca gcaaatcaga gaagtattcc
     1321 cagaacaaga tattttcagg attgatcatt atttaggaaa agaaatgatc caaaacatct
     1381 cagctatccg ttttgccaat aatatttttg aggctcaatg gaacaatcgc tatatcgaca
     1441 atatccaaat tacttttggc gaaaacctcg gtgtagaaga tcgcgggggc tactacgatc
     1501 atagtggcgc acttaaagac atggtgcaaa atcatatttt acaggttgta gcattacttg
     1561 caatggaacc gccagtagca ttttccgaaa aagaaatccg tacagaaaag atcaaagcat
     1621 tgaaagctat ccgcatttat aacgaagaag aagtgttaga aaacttcgtt cgcgggcaat
     1681 atgctcaagg tgaattggat ggtgtacaat tcaagggcta tcgcgaagaa gacaaagtgg
     1741 atgctcaatc agagactgaa acattcgtcg caggcaaatt cactatcgat aacttccgtt
     1801 ggtcaggtgt tcctttctat gtacgtacag gcaaacgttt gactgaaaaa gggactcgga
     1861 tcaacatcgt attcaaacaa gttcctgtta atgtattcaa aacatctgtc gatgaaccat
     1921 gtgacgatac gacacttcca cctaatgttc taacgatcta tattcagcca actgaaggat
     1981 tttctttatc cttgaatggg aaagaagtag ggcaaggatt tgaaacggaa ccaatcaaat
     2041 tggaatttag aaatagtgca gaaatggttg aaaatagccc agaagcttac gaaaaattac
     2101 tattagatgc gttgaatggt gatggaacga acttctctca ttgggaagaa gtcgctcaat
     2161 cttggcatat cgtggatgtc attcgaaaag cttgggacca aacacaaccg gattttccca
     2221 actacaaagc tggatcaatg ggacctaaag cagctttcga actacttgaa aaagacggat
     2281 tcgaatggat ctggcaacca gataactggt atcgtgaacg tggaaaatta aacgattgat
     2341 aacaaaaggg ggtgtgacaa agaacatgtc acaccccctt ttccctttta cttttcttcg
     2401 aatccttcag ctacagcttc aggatagaag tcttcaacga tttttgcaca acgaccgcat
     2461 agatgaggaa gtttttcatc tactcctaca tctttacgaa cttggcgaca gcgatcgcaa
     2521 gtttccccgt ctgctttttc cactgtaata gcaacatcat caaataccat agcgttatct
     2581 ggcacttgtt catctgctgc cataacttca aagtagtctg gtgaaacgat caataattgt
     2641 gcaatatctg catccaaagc ttttaacatt tcacgaactt gttgttttgg ataaacagtt
     2701 agttttgctt ccaaagattt accaatcaat ttgctgtttc ttgcttcttc caatgctttt
     2761 aagacattat cacggaagtc catgaaagca gtccaggtat ccatcaattc ttcttggttt
     2821 gcaaactctt cataaccagg aaactcgctt aactgaacgt actcctcttc ttctttcagg
     2881 aatgaccaga tttcttctgc tgtatgcgga atgataggag tcaataattt tgtcaaagct
     2941 acagctgttt gatagaaaac agtttgcatg caacgacgtt gataatcatc ttctgcttca
     3001 atataaacta catctttagc aaaatccagg tagaatgacg atagatcaac cgtcaagaag
     3061 ttcataactg tacggtaaat atgcatgaag ttatatttgt cgtacccttc ttctcggatt
     3121 tctttgatta cttggttcaa tcgaacggtc atatatttgt ctacagaacg taaatcattg
     3181 tacgaaacag tatctttttt aggatcgaaa tcttctgtat tcgctaataa gaaacgcatc
     3241 gtatttctga ttttacgata tacttctgcc acttgactta agatatccat agaaacacga
     3301 acatctgctt cataatctac actagatacc cataagcgaa ggatatctgc accaaattgt
     3361 ttgatcactt tttctggtgc aatgatgttt cctaaagatt tactcatctt acggccttca
     3421 ccatctaaag caaatccttg agaaataact gatttgtatg gtgctacacc attgatagcc
     3481 acactagtcg tgatacttga gttgaaccag ccacgatatt ggtctgatcc ttctaaatac
     3541 atatctgctg ggaatgataa ttctggtcgc tgacgtaaaa cagcttcatg agaagaacca
     3601 gaatcaaacc agacatccat gatatctttt tcttttgtaa attcgccatt aggagaacct
     3661 gggtgggtaa atccttcagg aagaagttct ttcgcttctt tttcaaacca gataattgat
     3721 ccatgttcag caaataattt tgccacatgt tcgattgttt ctggtgtgat gattgcttct
     3781 ccattttcag catagaaaat cggaagtggt actccccatg cacgctgacg ggaaattacc
     3841 caatcgccgc ggtcacggat catattatac aaacgtgttt taccccatgg gataacccac
     3901 tctacttttt ctacttcatc caaaatattc tgacggaatt tatcgataga tgcaaaccat
     3961 tgtggagttg cccgatagat cacaggcttt ttcgtccgcc aatcgtgagg ataactatga
     4021 gtaaagaaat ctagcttcag caaagctcct ttttctttta atagatcagt gatcatagga
     4081 tttgctttgt cataaaagat accttcaaat ccaggtgctt cttctgtaaa caatcctttt
     4141 gagtctacag gtgacactac agatagttta tatttgctac cagcgatata gtcatcttcc
     4201 ccatgtcctg gagcagtatg gactaatcca gtaccagcgt ctaaagtaac atgatcgcct
     4261 aaaatcacaa gtgattcacg ttcatagaat ggatgacttg cagtcatata ttccatttct
     4321 tgtccagcaa attcttttag aactgttaca ttttcccaac cgatttctga ttggactgtt
     4381 tctagcaaat ctttcgctac gacaaactta cggccatcgg cttccaccaa tacatacgta
     4441 aattttggat ttacagcaat accttggttt gctggcaatg tccatggtgt tgtcgtccaa
     4501 atgacaaaag ctgtatcttc atcaagaata tctttgccgt ctttgacatt gaaagctaca
     4561 aaaatagatg gagattttac atctttatat tcaatttcag cttccgctaa agaagactca
     4621 cttgacggag accagtaaat tggtttcaat cctttgtaaa tatagccttt ttcagccatt
     4681 ttaccaaata cgcgtacttg tgcagcttca taagaaggat ctaatgtaac ataaggatgt
     4741 tcccaatctc ctgcaactcc taggcgtttg aaatctgctc gttgcttatc tacttgggaa
     4801 agtgcatatt cacgacattt ttccaagtat tctgccatgg acatttcttt acgtttcacg
     4861 cctttattag ccaatacttg ttcaatcggc aatccatgtg tatcccatcc tggaacatat
     4921 ggtgcacgat agccagacat tgattttgaa cgaatgataa tgtctttact gattttattc
     4981 aatgaatgtc ctaaatggat attaccattg gcatatggag gtccatcatg aagaacaaag
     5041 ctaggttttc cttcgttcaa ctcttgtctt ttttggtaga ttcgattttc ttcccattct
     5101 ttttgccact cgccttctcg gtttggcaaa ttaccacgca tagggaattt tgtttttcct
     5161 aattgcaagg tttctttcat tttcattcaa ccatacactc ctttatttgt tttccggaca
     5221 caaaaaagca tgttcagccg caagggacga acatgccgtg gtaccaccca aatttagttg
     5281 catctatcta gtgcaaactc tctgtgacca ataacgaggt cagccgctgc ttcttactct
     5341 aatttcggaa aagcaggtct caagtagagg atctttcacc atgcagtgtc ttaccgaact
     5401 tccactctca tcggctcgct ttaagaataa caaagtgctt ttctgttcta ctcgttttca
     5461 ttttcaccag gcacgatcga gatatcaata gcctgtgtat caaatgccgt cataggtgct
     5521 tctatgactc ctgtgtctga gtttacatca gtatcatttt cattgtcaag ctgttttgct
     5581 aaaacttctt tgatcacttc atggctgtca gtcacgtagc tggagaatgg ttgtaaaatt
     5641 tcatcccatt ccggactttt cacttgttct aattgagact caagcatcaa gctgatacgt
     5701 tgatggaata ctcgtgtttt tttcttaaga tcttcagttt ctgtcgcaag ctgtcttgct
     5761 ttttctgttg catctgtcaa gatttttctt gctttttctt ctgcatcagt tgttaattga
     5821 tgtgcacgtt tttcagcgtt cgcaactaac tcatctgctt tgttttgtgc agaagtaacg
     5881 atcacttcgg attccttgct tgcacttgtt tttactttat cagccgtatc ttgagctacg
     5941 atgattgatt ggttcaaagc atcttttaat tcattgaagt actcaagttt ttcttcagaa
     6001 tgtttgactg ctttttctaa ttctcgattt ctttgagtca attcctcata gtcacgaaca
     6061 actaaatcta agaaatcatc tacttcgtct tgattatatc cccgcatttt tgtcgggaat
     6121 gtcttatttt ggatatctaa tggagttaaa gccatgagta aaccaccttt ttattttatt
     6181 tacgtaatac accgaacagt accctgtact tttctttttt cgtcttcccc tcaagttctt
     6241 ggatctggac tctgccataa cctctgactg aaataatatc taatagttca agtacaaaat
     6301 caggtcttgt attttctgtc cagttcactt ttactttacc agcttcgatc agctgcttgg
     6361 accgctgtct ggaaatatta taaatctcag agatcacact gtctagtcgc atggaactaa
     6421 ctgttcctct ttcttgtgtc cagctatctt tcggcataat catttctgtg tatttgattg
     6481 gttccaagcg tacagcaacc tttccaatct ttgttacttg tgcagcaaca aaatgatcca
     6541 cctcatttgc aataaatact tgccagcgat caccgtccga gatgatatcg ccaaagtaag
     6601 agcgtttgat ccccgttccc actaaagttc caaggatttt cccatgactt aggctggcaa
     6661 atttgctagg ataatggatt tcatacaatg ctgtttcaaa atcttcctct acaggttcgt
     6721 aataatcggg gaaaatcaaa catctttttc tttctgcctg ttcatatccg ccataaaaca
     6781 tgaaacgcaa atcactgctt tgtcgaatca gtgtttctaa aatataggat tgtcttgggt
     6841 caagaaattc ggtcagatag ggtgcatatt gtccttgaac ctgttcaatc caatcgccta
     6901 ccgaatcgat aaatgtacgt tcatctgccc gaaagtgctg gtatacattt gcatccaaaa
     6961 ttttcccctc ctatctattc tgagaataaa gattttgttg cttcaatcaa taaactaaat
     7021 ggacagttat ccatataacg atctgtgcta aaccttgtac ggctaagttc aagatcagga
     7081 ttgcccctaa aattgtgaaa tccaagggtc caatcctcaa aggcagacga tcgaacaaac
     7141 tgatatacgg ttcgcagatc ttagctaata atcgtcctag ccctgattga tacccgcctg
     7201 gaaaccaaga gagcaatgca tataccatca agataatgct atagaaatag gctgcccgtt
     7261 gaagtaaatg aagtaatagt gcaatagtat aaatcgtagt tcctcctctt acagatcaaa
     7321 caaattcgca tctgcaaaag attgagcggt tgaactgtca atctccatcc cagtcggtgt
     7381 acaaagaaaa atctcatctc caacacgttt gatatctcca tctagtgcat aaactgttcc
     7441 tgttagaaaa tcaacgattc ttctcgcttg tttttcttct actaaatgaa aattcactaa
     7501 ggttgcttct ccagcaatga ttcgtttagc aatcgccata gcttcagaat aagctcttgg
     7561 ttccattaca gtgatttttc cagcaagatt tgctttaggt tgtgtagagg ttcttgaagc
     7621 tgttctttct ttcgctacag tttttgtagg tgattgatgt agttcaatta cttttttctt
     7681 ttcagccggc tgaatacttt catgcgttac aggacgtgaa gtcgccgttt tttgtttttc
     7741 atgagtattc gcagcagata cagaatgagt tgattgattc tgtgttgtat tttttttagt
     7801 tggctgtaca gccttttttg ttgtttcagg ataatcttgg tattcgtact cttcctcatc
     7861 agctaatccg aaaaaattag caattgctcc tctgtttaaa agcggcattt ttgttcctcc
     7921 tttattcgtc tttgaatagc gctgttccta cacggataaa tgttgcacct tcttcaatcg
     7981 caatttgaaa gtcttgactc atccccatac ttaattcact acatggtgca tgggcataac
     8041 cttttgcttg aacaagatca cgcaattctt tcagcgtacc aaacacttta tggatttgct
     8101 catcagaagc ttctttaggc gccatagtca tcaacccaac aaccttgatt tttgtgcagg
     8161 cagctaaaga ttccacaaac ggtaaaagct catctggtga aattccatgt tttgattcct
     8221 caccagaaac atttacttca ataaaacagg ataattcttt ctctaaccgc ttctggattt
     8281 cttctgctaa tcttaaactg tccaacgcat gaaagtaatc aatttcattt acaatcaatt
     8341 ttacctttct gcgctgtaaa ttaccaatta aatgccactt tatttcagga aaatcaaata
     8401 aatcttgttt cttttctaat aatttatcta ctcggttttc tgcacaattc ataacgccag
     8461 ctctaaccaa ttcttcagct tgagcggatt caacagattt tgttacagct actaatgtaa
     8521 cttctttcgg atcacgatga acaagagcac acgaatcctg aatttcttgc ttgattttct
     8581 gcaagttgtc agcaatcatg tgctcttcct ctcctttatt ttttacgacg gaaaaacggt
     8641 ggcgtattta attcatcatc gccatgagat tttgtttctt cgcggttgaa tgtatcaaat
     8701 tctttttttt cgatattgtc aaaattggaa tcatcaactc taggacgaac attttgttca
     8761 cgtctgatat cccaatcgcc aaaactgttt tcttcttctg aagaagttgg tttagcttga
     8821 tccatatcta atacagtctt ttgagcaggt gattgcattt gtgcttgtct agctggtcgg
     8881 ctagacttgc gttccttttt tgattcatcg atacctgttg cgatgaccgt cacacggatt
     8941 tcatcgccca tttcttcatt gatagatgta cctagaatga tatttacatc gccagttgct
     9001 gcatttgcta cgatatctga agcatcttgt gcttcgaaca atgtcatatc caaaccgcct
     9061 gtgatgttaa gaagtacttg ttcagctcca tcaatagatg tttcaagcaa tggagaggag
     9121 attgcttttt tcgttgcttc tactacgcga tcttctccac tagccacacc gatccccatc
     9181 aatgctgtac cttggttttc catcactgtt ttcacgtcag caaagtcaag gttaacgtaa
     9241 cctggcgctg tgatcaaatc agagatacct tgtacacctt gtcgtaaaac attatctgct
     9301 tcacggaaag cttcaagcat cggtgtcttt ttatctacaa cttctaataa acggttattt
     9361 gagataatta ataatgtatc cacattttct ttcaaacgag cgataccttc tgcagcaaaa
     9421 cggccacgtt tcggtccttc aaaagtgaat ggacgtgtca cgacaccaac agtcaatgcg
     9481 cccaattctt tcgcgatacc tgcaacgatt ggtgcagcac ctgtaccggt tcctccaccc
     9541 attcctgcag tgatgaaaat catatctgca ccgtctaaag cttcgcgtaa ggattgttca
     9601 ctttcttctg ccgctttttg tccaacttct ggctgagaac cagcacctaa accacgagta
     9661 tatttggggc ctaattggat aactgtttca gcttttgaat tttttaatgc ttgcacgtct
     9721 gtgttggctg taatgaattc aacaccttta acgttttctt caatcattcg gtttacagca
     9781 tttccaccgc caccaccgac tccgatgact ttgattactg cgccgtcatt gatattatta
     9841 tcaattgaga attccatatc ttattcctcc caggatgaat taatcaaaaa tatttgtaaa
     9901 gaaacccttg attttatttt ttacattttc tccggactct tttggttccg gctcatcgta
     9961 gattgtttct tcaggtgctt catagctctc atatgtgttt gttgtttctt gttcaactac
    10021 catttgacga gctgctgagg tttcacccgt aacagcaatt tttgccaatt gatacacttc
    10081 actcaagttt gctgaataat ccacgatact tattacattt gtaaataccg ggtttctcag
    10141 ccccatgtga tttggaacat acaattttac atttacgccg aagatttctt gtgccaagtc
    10201 cacaacacca ggaagactag cagctccgcc tgtcaatacg acgccacctg gcaactctaa
    10261 tgcttcgatt tggtccaatg catcttttgc tttgttgaaa atctgttcca ttcgagctga
    10321 aataatttct gacaagtagc gttcatcgac tttcactggt tctgactgac caatcacatc
    10381 tactggaaat tcttcatctg gtgatgtccg ttctgggtat gcatccccgt agtttatttt
    10441 caacgcttct gcattgttaa atgacgtatt taatacaata gagatatctt tagtgacaaa
    10501 ttcgccacct tcttggtcaa gattggtaaa cttcaattgc ttatcatgca taacagccgt
    10561 tgttgtttgg ccgccaccca tatcaatgac gatcgtacca aaatcttttt ctccgtcaga
    10621 aagaattgat tcagtcagcg ctaaaggagt aatcaccatt tcgttgacga ttaatccggc
    10681 attttccacg catttacgta tgttatggat gatcgtttta ggtcctgtaa acaacagacc
    10741 gtacatttct aatcttacgc cgatcattcc acgaggatct ttgattccct cgaacccatc
    10801 gacagtaaaa tcttgtggca atattgacac gatttgacgt tcaggaggta ttgaacgaac
    10861 aagggcagct gaagccacat ttctgacatc ttcatcggtg atttcctttg aatctccgtt
    10921 aactgcaatc attccctggc aattttctac ttcaagcatg tttgctggca aaccgacact
    10981 tacgcctttg atctggatac ctgctttttc ttctgcttgt cgtactgctc gttgtatcgc
    11041 ttggaccgtt ttatcgatat caacgataat gcctcggtta atcccttctg attttgcgtt
    11101 tcctactcca ataatattca tttggctgtc gatatattca gccacgacaa ctttaacaga
    11161 tgtcgttcca atatcaaggc ctacatacat tcctgttttt gccatgaatg ggattccctc
    11221 ctacttacta ttccatatac aagcgaacaa ctagcgtccg ttttaaaata tacttattaa
    11281 tagatatatt cactgcttcc aattctacca taaattactg gtttttagaa agtacattct
    11341 tactttttaa agttgtttta tcaattttca tttaaacttg acggagttgt acttggatta
    11401 tcttctattt gacttgtttc acttgaatca tttgaatcag atgatccgtt tgtgctagac
    11461 gatccaggtg tttgtgcttt ttgcgtttct gcctcttcac ttgattcgtt tgtttgatcc
    11521 gcattttctt cactttcagt tgtttcttcc gattggcccg attcttccgt ttcttgagtg
    11581 ctaccgtaag gatgcgagaa gataccaact tccatgtcga tcacaccttt ttcatccata
    11641 tctttggcta cttgcgaata atatttcatt tggttcgcaa gattattgat atttacaata
    11701 acttgattgc cgtcattcat attcaattgg atcagatttt tattgcttgc tcttggcgtg
    11761 tacttgattt cagaaattgc tttttgtaat tctgatgata actgattgta ttgctttgtt
    11821 aattctttga tttttgcaga gtctttgaac tcttccaaga tagggagatt ttttgtagga
    11881 tttgctactt tttcatctaa tactgttcca ttttcaatca ctgggtagta ttgattgttt
    11941 tttgcgatca aagcaatctc tttatattct gttacatcca aatcgaattc attcatccct
    12001 ttgaaatgta tatttgctgt ttcgattctt ggctgtgctt ttttaaggcg atcgatataa
    12061 gtagatcgat gaaaatactg tccccaaaca ttttctccta atgacaattt tgtatcagag
    12121 atgatttctt ggctgttgac tgtcttattg cctgatactg caactgcttg taatttgcta
    12181 taaggagaaa cataatagat caaaaaaatc agcggtatac ccaatacagt gatgatcaaa
    12241 gctaaccgac gaaatagtac tttgtgccgt tgacttttta aattgggcag ccggttcaaa
    12301 aaagaaccgt tatatggtcc accgtttttc ttttgcttga tctcttcaaa aaattcgatt
    12361 tttttctcgg actggctttc ttcttgctgt tcctcttgaa ccgttttttc ttcttcagaa
    12421 tctgaaaggg gtgtatctaa tgttatgtct tctttttttg gctcttggtt tttcatctct
    12481 tgttctgcca gatattttcg gttagccttt tgccaaggag tcaaattgtt ttcatcagaa
    12541 gacggatgag acaatttttt tggttctttt ttcttactca ctaagaccac cgcctttatt
    12601 ttttgattat ttgttttact aagtcataaa gacgttttga agcatcaggg attccttgtt
    12661 ctttagaagc tcggcacatt tgcttttgca attcttcatc acccatgatt tcgttgactg
    12721 tctgacttag tgattcacct gttagttcat tatctgcaat catttttgct gctcctgcgt
    12781 gaacaagact cattgcattt ttagtctgat gatcattcgt aacatatggg ctgggaacca
    12841 acactgccgg taaccccaga gctgtgaatt ctgcgatcga agttgcacca gcacgcccca
    12901 ccagcagatc actggaagcc atgacctctg ccatattttt gatataaggc tggacactaa
    12961 tattttgtat ttcaggattt attttattta ggatctcttc ataatatcta tctcctgaag
    13021 catacaaaac ttggtaagaa gaaggaatat attcacttaa aaaaactgat actgcttgat
    13081 tgattttaag tgctccttga ctaccgccaa aaatcaagac tgttttcttt tctggatcca
    13141 aatcaaatgt tttcaaaata tctgattttt tagtatctgc tacttcttgt gctcgaggat
    13201 tacctacaag aactgctttg ttttcaggaa aaaaggaagc cgcatcttta aatgaaagag
    13261 cgatccgatc cacatatcgg cttaaaaatt tattggtaac acctggtaca ctgttttgtt
    13321 catggataac tgttggaata cccattttgg ctgcagcgta cactaccgct ccggaaacat
    13381 atcctccagt accgataacg atatctggtt gaaaatcact gaggattttt tttgcttgac
    13441 ggatactctt aagaaacaat cggaccgtct tgatgttatg catggaaatt tttcgtttaa
    13501 atccttgaat ctccaaggta tggaaaggca ttcctgtatc agggacgatt ttgttttcta
    13561 gacctcgttt tgctcccaca tacatgaatt cagcattcgg ttcttgagtc tttacataat
    13621 tgacaaaggc aagagctgga taaatatgtc cccctgttcc tccgcctgta accaaaattt
    13681 tcatttttta tcgctcgctt ctactttttc tattttaatc ggttcacagc ttccatgaat
    13741 ctgtttcctc tagtttcaaa atttgggtat tgatcccagc tggcattcgc tggtgataat
    13801 aaaactacat caccatctgt actgttttct aatgctaaag gaactgcttg ttctacagaa
    13861 tcggctgtta ctattgtctc gatgcctgct ttttttccag catccatcaa cttttctttt
    13921 gtttggccaa atacgaccat tgctttgatt ccttttaatg aaggaattaa ttcatcaaat
    13981 gtatttcctc tgtctaatcc gccagccaat aacacgacct tagacgtttc aaatccactt
    14041 agagccattt tagtcgctaa aatattggtt gcttttgaat cattataaaa ttttctgcct
    14101 tggatttctc ctacatattg cgtacgatgt ggtacgccat ggaaaaaatg caacgtttct
    14161 cgtatagctt catttgatat accatacaat tttgctacag aaatagcagc tagtgcattt
    14221 tctacattat gcttacctgg tacgccaagt tcagatattt ccattatttt ttctttttta
    14281 tagtatattg agtaatcgtc agcatacaca ccgtcttcca acacttcttt tgtcgagaaa
    14341 ggcagtacgc gtgcttttgt tctttgcgct aattcttgta gttctgactg attccagtta
    14401 aggatcagat aatctttttc tgtcatgttt ttttgaaggt tccatttagc tttgacatat
    14461 tcttttcttg taccatgata atcaatatgt gcttcataga tattggtaat caccgctata
    14521 tgagggcgga aatctgtgat tcccatgagt tggaagctag acaattccat cacaatttta
    14581 tcttctgatt ttgcttcttg cgctacacca cttgctggat acccaatatt acccgctaaa
    14641 cgtgcaattc cttgatctgc tcctgcattc agtagtagtc ctgtcatcgt tgttgtcgtt
    14701 gtttttccgt tcgttcctgt aatcccaata attgggcatt ccgctacttc gtaagctagc
    14761 tcaacttccg taattaccgg aatacccatt tcttgggctt ttgctactaa aggatgactg
    14821 tagggaatcc ctggattttt gaccatcaat gaaaattctt catccaacaa ttcgattgga
    14881 tgactacctg ttattacttt tatccctaaa gaaagcagct cttgtgcttc cggattttcg
    14941 tcaaaaggtt ttccatcatt aacggtgaca agtgcgccca attcatgtaa taatttggcc
    15001 gcacttactc cgctttttgc gagtcctaag acaagcactt tcttattttc atatgttgat
    15061 atttttttca tttcccgtca tctcctacca aacgatccat aatgtgatca acgaagtcac
    15121 aatgctgatc aaccaaaaaa ctgtatcaat cttccactcc gaccatccgc acatttcaaa
    15181 gtgatgatga attggagaca ttttaaaaat tcgttttcct gtaagcttaa aggaagtaac
    15241 ttgaagcatc acacttgctg tttccataac atagatcaaa ccaatcaaaa gcaatgtcca
    15301 ttcttggttt agcattatag aaattgccgc taaaagtccg cccaacgcta atgaaccgac
    15361 atctcccata aaaatcttgg caggttttct gttgtagacg aagaaaccca gtaaacctcc
    15421 taaaacactt aagcaaatga ttaatacatc atactgatgc tgatgccaag caattagacc
    15481 gtaagctgca aaagagatac ttcctaagcc agcaactaat ccatcgatgc catctgtcag
    15541 atttactgca tttgaaaatc caacaagcca gaagagagcg aaaataccat aaatgattcc
    15601 taaaggtaga tctagtccga agaaattcaa tacacctgga taaccttcag aacggtaaac
    15661 aagataaaag atgattcccc ctagaatctg tccaatcaat ttctgcttcg agttcagtcc
    15721 catgtttctt ttcttgaata tttttataaa atcatcaaga aagccgatta ctccgtataa
    15781 cgctaataca aaaagcaaga tgaaaagagt aggagttagc tgtttttgcc aagctccaac
    15841 ccaaattcct gtcaaaatag agccgatcag aaacactaag ccgcccattg taggagttcc
    15901 ggctttgcta ttgtgccact ttggtccctc ctcacgaatc gcctgacctt gctttttcat
    15961 ttgaaaataa ccaataaata gcggcattgc ggcaattgtc attgcacaac tgctgacaat
    16021 gggaatcaat gcttgtgtcc attccatgct cttttctctc ctatttctct tcttattctt
    16081 ctaaagtaaa actaagtttg tcttcagtga ttttttcata tggagcaagt tcttgtttga
    16141 cacaataacc atctccatca aaactaactt caataccgag gattttcccc agcttcatga
    16201 tatctgcttt tgaccatcct gttacgtcag gcatcagctt atcaccacca gtatatagga
    16261 tcagtttttc tccagagatc aactgatcac cgttggcagt agattgtttt tgtacttttt
    16321 taccattacc gatcactaca ggttgcagtc cacttttttg agcatcagct gctgcaacgt
    16381 ccgcttcaag attacgataa tcggctacgg atactttttc tgtttttgtt tctgtatctg
    16441 aatcttcttc agtttcttta aaatccattg cccgtttcat caatggattt gcgattttag
    16501 ccaatgccat tcgatcatat gtcttagggt gtttcattgt tagatacaag acatagtctg
    16561 gatcttcaga aggaaccatc tccacaatcg aatacaggta ggctgtatct cctgtttgat
    16621 atccccctgt atcagaagcg atttgggctg ttccgttttt tgccgataca ttgtatccag
    16681 gtacactata tacaccataa gcactaccgt agttttcgct ttcgacaaca tctctcatat
    16741 attcacggac tttttctgtt gtttcttttg aaaaaggttg ccctagaact tctgtttgag
    16801 ttgttcgctc ttctccagtt tggggatcca ccactttgct gatatatcga ggttgaatca
    16861 ttgtaccgtt attcgcaata gaagtgaaag ctttcatcat ttggaaattc gtcacaccta
    16921 ccgcttgtcc ataagcactc attgctcggt cgacaatatt taatgtaggt aaagcaccgt
    16981 ttacttcatc atctaatcca gaatgagtac tttgtccaaa tcccagtttt tgtaaatagt
    17041 tataccatcg tccgcctaat cgctgttcca gtatgaccat cccgacgtta cttgaccatg
    17101 aaagagcttg acgcatggtc agcacacctt tttcaccaaa gtcatggtca ttgatcgtag
    17161 catcttctac ttggattttt ccagattgga aggtttcatt ctcgttgaat tctccttctt
    17221 cgatagcagc tgcagtagtg aaaactttca tggttgatcc tggttcataa ctatcttgta
    17281 ccaagaaatt acgccagata gcatcttttc cagtcaatcc ttccattgtt tcaggattga
    17341 aagtaggtcg ttgtcccatt gccaagattt ctccggtttt tgctttcatc aggactgctg
    17401 tcaattcttc tggctgatat tcttcattta cttgatccat cagtgtttct aaataacttt
    17461 gtagtctgct gtctaaagtc gtgtagatat cttgtccgtc gacagctttt tcttcttctg
    17521 ccactgtacc tggcagagga ttttgaaaat tatctttttg ataaataatc ttaccatctt
    17581 taccgctcaa gatatcattg taagcagact ccaaacctag tttaccaact aagccattct
    17641 catctttgtc tggaactgcg tatccaataa aatgagaaga aaagacgcca tttggataca
    17701 tccgagcctg atggtcgaca aaataaagac cagcgacacc ttcttttttc atgtccgctt
    17761 cgatgttttg cttggtttct tgagagatat ttttcccata gctaccaaat tcaacttgat
    17821 acagctcttt ttttgcgccg tcctctaaaa tattcagtgc atcttttttg tctatagaaa
    17881 gattcttatg caggatttct gctattttat caaaattctt ttcttcaaca tacagttttt
    17941 tatccccaga ggtatatgtt tttgagagta tcgctttaat tgaataagag gaggcatctt
    18001 ctgcgagcgc cacaccgtta cgatcataaa tcgttcctct ttttgctttt actacttcac
    18061 ttccttggta caactgtttt gttttttcag ctaatgaggt accagcaaca tgacctccaa
    18121 taacgatata cgagaaacgg acggcaaata aaaagaacaa tccaatactc gtagcaaata
    18181 aaatgatacc tacttttttg cgattgttca tcggatttag atttttcttt tccataaacc
    18241 gccgaatttt atttttaaga ctcatttact tcactttcct taaattgtcg tcattaatag
    18301 acaatccttt ttcttctgcg atttgtttaa tccgttcagt acgagaaagt tcgttttttt
    18361 cttgttctaa actcgttttt tcttgctttt tatcattgat ttctgtttgt attgacgtga
    18421 tttctttttc agctccgctg atatttgtgc gcagcataac tgttaaaaca cataaagcaa
    18481 caatagcaat cgccacgctt gccacagcaa ttttttccaa agcagaaatc tgctttaatc
    18541 gttttgccgg tgattgtgga agaatgattt cttgattcga agaagaatca ggcatttgtt
    18601 gttccggctg atccaccaat tcaggtaaat catattcgta atcttgcatt ttctttaatt
    18661 ctgccatcat tctactcctc tttcattctt attttttctg caatacgtaa ctttgcactg
    18721 cgtgatcgat tgttttctgt tagctcttcc tctgaaggaa ggattggttt acgagtaatg
    18781 actttcagtt ctggttggaa ttcgtcaggt atgacaggaa gtcccggtgg tagatcttgt
    18841 accgtactat attctttaaa catacttttg acaattctgt cttccaatga atggaatgtg
    18901 attacgctga ttcttccatt gactttcaat aaccggattg cctgttctaa agaggcctct
    18961 tcggcaccga gttcgtcatt tactgcaatc cgcacagctt gaaaaattct ttttgcagga
    19021 tgcccgcctt ttcttcttgc gggagctggg atgacttgtt taatgatttc gacaagctca
    19081 ccagttgttt ggattggttt tgtttttcgt actcgttcga tttcacgagc aatttgtttg
    19141 gaaaatttct cctcgccata gcggaagaaa atctttacta attcatggta ggaatattca
    19201 ttaattacgt gataagcgga taaaggagct gactgatcca tgcgcatatc caacggtgca
    19261 tcttgatgat aactaaatcc acgttctgct tcatctagct gaggagagga aacccctaga
    19321 tcatacaaaa tgccgtctac cttatctaca tgctcactca agcgttcttc taattctctg
    19381 aagttcgctt tgataaatgt gaccatacct tgttcgacat aagaagctaa acgcagcttg
    19441 gcgtgatcga ttgctttttg atcttggtca aacgcataca gatgcccttt agaacttagt
    19501 tggcttaata aatattcact atgtccggca ccgccgagtg tacagtctac ataaacgcca
    19561 tccggtttga tgttcaatcc atctaccgtt tctttcaaca tgactgtata gtgttgaaat
    19621 gtttcagtca ttagtcagcc tcttcctatt cttatagtcc gaaatcaatc atactttcag
    19681 caatttcgtc aaagttctct tctgctgctg cagaaaaatc ttgccatcgt gtctcatccc
    19741 aaatttcaat tcggttagat acaccgatga ctacgcaacc ttttgtcaaa gctgcataat
    19801 ttcttaacgt gctgggtata ttgattcgtc cttgtttatc aagttcgcat tcagtggcag
    19861 ccgaatagaa gaatcgcaca aaagtacgtg cgtctttttt agccaacggc atttcgttca
    19921 gtttagcttc cagattttcc cattcgctca tcggatagcc gaaaagacag ccatccaatc
    19981 cgcgtgtcag aacaaacttt tcgccaagtt cttcacgaag ttttgagggc acgattaaac
    20041 gtccttttgc atcaatatta tgttggaatt cacccatcaa cattgaaagc cccccattcc
    20101 tccactaact aaaattagtc taccacaatc ccccactttc taccaccttt tctgggaaat
    20161 ccgggaacaa ctttttttat tcataaacat ttttttaaaa atcaagctgc aaaaagcctt
    20221 ttaaaagact ttttacagct tttctatttt tttcataggt aagaaagtat gttcacaatg
    20281 atcaagacag cgtacaagac gattgtcaac agaaaaacca aacgccaagt catcttgaaa
    20341 taacgcggat aaataatttc ttcataataa tatgcttgga aaacagcaat acaaattccc
    20401 attagcaaaa cagaaataaa gaaaaacggt aagatagaaa ttgtccctgt atttctagac
    20461 aatgcatgca taccgcccca caaaaaagga atcgccaaat caggggcttt tattttaaaa
    20521 cgacgattta acgaaaataa ttttacaaga aattggcaac caaacaaaac gattaccggg
    20581 taaatatacc aaaacaagat aagtggtgtg aatgtgccca tgaacgtaaa ctcctttttt
    20641 atcgttgctt caagtatact gtatatactt tacacaactg tttcatttta tttaaattta
    20701 tgcagttata ttctatatct tacaaacaca ttccatgtat tccaattccc tatccgttat
    20761 aataaaagaa acaggcaaca ggaggaaaat catgatcaaa tattttacat tagataatga
    20821 gaaactgcgt aataccggaa aggaaactga ccaaacgatc tggtattgtg tagaacgtcc
    20881 taatcaagaa gagattcatc agctgacaca tcaatttgaa attccgtcag attacatcac
    20941 ttccatattg gacgatgcag aaaattcccg tacagaagaa ttcaatcaat caaagttgac
    21001 aaagccagcg ttattattgt tacaatatcc ttttgctaaa acaagtccta gcggttattt
    21061 tcaagtggat acatacccgc tctctattat tttgacgccc aaaaagaagc ttatcacaat
    21121 tacgaatcat gaaccactgt ttttaaagaa agtctttagc cagacctttc caaaaagtga
    21181 tatttcgcct aatctcaata tcttctttca attcatttgg caggtcacac tctcttacaa
    21241 cagttattta gctacgcttc aatctcaatg cgatactttg gaagccaaac ttcaagtatc
    21301 cactgaaaac aaacaacttt atcaaatcat ggatatccaa aaaagcctcg tttatttcag
    21361 agaagcgact tctgctaatt tagatgcatt gaaagaacta gcgcaaaata aacttttcca
    21421 agaaaatcat gctttgaaaa atcatttacg tgatgtattg gtggaaacag agcaggcaat
    21481 gactacttct cgcattcaat taaaactagt agaacaaatg aatcagactt tttcagcaat
    21541 cgtatcgaat aatctgaata atattatgaa aattttaact tctttaacga ttattttaac
    21601 tataccgaca atcgtcggca gtatctatgg aatgaacatt aagttaccta ttgctgaaag
    21661 ggatgatgcc tttatatggc tagttctatt aacattattt atttgtatcg taactaccta
    21721 ttatttaaaa aaagggaagt ttttataaaa aagccctctt gtggaaatgg aaaaaagatt
    21781 gtacacttga atcaactaat taatgacgag gttgaaaaca atgcgtgaaa aaaaacaaac
    21841 aagtactttt gctaaagtaa caaaatttgt catttggaca atgctgattt taacgattgg
    21901 atctgttgta ttgactgctg taatgagtgt catgtaaaaa taaaatatag ctatcagctg
    21961 ctgaaaagga cctgaaccac tgcgaggtgg ctcaggtcct ttttgcgagt ttcaatagtt
    22021 cttttgcatg ttcgagtgtg agttttgtaa tctcacttcc agccagcata cgtgcaattt
    22081 cttcttctct ttcttttgtt ttcaactctg aaacagaagt ttgcgtacgt ccatcaatca
    22141 ctgctttcac aatataatat tgatagtcgg cgactgcagc aacttgaggc agatgagtga
    22201 tgcacaaaac ttgagagtat ttcgatattt gagaaatttt atctgcgatg gcctgtgcaa
    22261 ctcttccgct cacacctgta tccacttcat caaaaataat acttgtcaca ccttgctcag
    22321 aagaaaaaat agttttcaaa gcaagcaaca tccgagacag ttcgccacca gaagcgactt
    22381 tcaccaaagg cttcagtggc tctcctggat tggtcgtaat gtaaaattcg atttggtcaa
    22441 atccatcatg attcaattgt ctattttctt cttttaagaa acgtacctca aactcagtgt
    22501 tttccatata cagacttttc aattcatgca gaatggattt ttccaattct tttgcgattt
    22561 ttttacgcgc ttcatgaaga tctgcggctt gttgctgaag tagaagctct ttttgctcta
    22621 gttcctcttt cattttgtcc aattgacctt ctgaaaaatc agaagaagct agctcttcgg
    22681 tgatttcgtc atagtaagcc aatattgact caatggattc gccgtattta cgtttcaact
    22741 gtcggattaa ttctaaacgt tgcgtaactt cttctaaacg actctcatct aactccaata
    22801 aatcaatctg acgactcata tcgccgattg catcttgcag caaatagtaa gcactttgga
    22861 tattttcata aattgcttca tactccacat ctagatgggc gatcccttgc agttctgaaa
    22921 cggccaagcc aacaccatct acactgtttt gttcattatt cgtcagtgct ccgtaacctg
    22981 cagctaatcc atcagcaatt ttttgataat tgctaagttt ttctctttct tcttttaatt
    23041 tctcttcttc tccgacttgc aaatcagcag cagctatttc ttcttgctgg aaatggagca
    23101 tatctattct ctgaacatag gatttttcat tttgctgcac ttttcgtacc cttttttcca
    23161 gctcacgata agagatatat tcttgctgat aactcttttt cttctgttgg aaagctacat
    23221 ctccaaaacg atccaataaa gcaagatgtg attcaggctg gagcagttcc tgatgttcgt
    23281 tttgtccttg gatatctact aaataactgc cgattcttct cagattagct aatgtgataa
    23341 tatgaccatt cactcgacaa acattttttc cagttaagga catatcccgt cgaacaatca
    23401 gattgtcctc atctgtttcg atccctaatt caaccatcag ttcagagaat ccttcttgtt
    23461 ttggtagttc aaacaaccct tcaagcacac atttttctgc tccttgacgt atataatcgc
    23521 ttgaacctct tcctcctgct aacagaccta aagcatcgat aataatggat ttacccgcac
    23581 cagtctctcc ggtcagcgcg gtcattcctt cgtgaaaaga aagccgcaat tccggaataa
    23641 tcgcaaagtt agtaatactg atttcttgta gcatcacatt cgctcctcaa cttaaaggta
    23701 atgcaggaag tctttcttca ataaaactgc ctggtcttca gttcgtgtaa tcatcaaaac
    23761 attatcgtca tcattgataa tcgaaaataa ttctttcttg taacgtttat caatcaaatt
    23821 tgctgctgcc gaagcatttc ccggcaatgt tttcaaaata acaaattttt ccatttggtc
    23881 aactgccacg aaagcgtctt ttaatagttt ttccaattta gtatttacat cttcgtttgt
    23941 ttcagctggt atgctataac ggtagccgcc atctgaagcc ggtaccttga tcagtttcag
    24001 ttcctttata tctctggaaa tcgtagcttg agtaacagat attcctttgc ttttcaggat
    24061 ttcaacaaat tcttcctgct ttcggatatc ttgctcactc aacaatcgag taatcaaacg
    24121 atgccgatct ttttttctca ttacgtcttc gctccttagc atattcttct taatcaatga
    24181 tagcttattt ccttggtata ataaagaaga atccgcatac ttctcacttg attttcacag
    24241 ttacaattta tttatggtca aactgttcgt gtgcatttga aaccacttct gccatattaa
    24301 tagctgaatt gattgttccg ctttcgggta ctttcctcaa gtgagccaag aactcgatat
    24361 ttccttctcc ccccgtgatc ggagaataac ttaattccag cacatcatat ccaccttgtg
    24421 ccgcaaatga caaaatgtct tctaaaactt tttgatgggt ctgagggtca cgaacaattc
    24481 cgtttttccc tacagcttct ctgcctgctt cgaattgggg tttgattaaa gcaatcactt
    24541 ctccctcttc ttttaaaata gcatgtaatg gaggcagcat cagttttagt gaaataaaag
    24601 aaacatcgat aacagcaact tctggaattc ctaaggtaaa gtcttcgggt tttgcataac
    24661 gaaaattcac tctttccatt acgacgactc taggatcttg tcttattttc caagctagct
    24721 gattgtagcc aacgtctaaa gcataactca tttttgcgcc attttgcaaa gctgcatcag
    24781 taaatccgcc agtggatgca ccaatatcta acaatgtttt tccttcaaca gatagatcaa
    24841 atgctttgag tgctttttct aattttaatc cccctcgaga aacataagga agttttttac
    24901 ctttgactct taattcacta ctcactggaa ttttttctcc cggtttatca aaccgctcgt
    24961 ttttttcgtt gtaaatcaaa ccagccatca ctgaacgttt ggcttgttct cttgtttcaa
    25021 ataatccttg tttgaccgct aatacatcta cgcgttcttt ttccattctt tccctcattt
    25081 ctccacatca aattgcttga tcatctggcg catcagcccg ccatcaaaag aaaaaacgtc
    25141 ctcttctaat ttgtctatga tcttgttggc ggataacagc tcaatctcca acgcctcacg
    25201 tgtcttttct aggcctaata aacgcgggta agtatttttt tccatacgtt catctttacc
    25261 agctgttttc cctaatgttt tcgttgtacc aatcacatcg agtagatcat ctcttatctg
    25321 aaaagctaaa cctaaatgtt cggctaatcg ttgaagtagc aataagattt cttcaggttg
    25381 cttcgctaat atgccaccag ccagcaaggc ataacgaatc aaatgccctg tctttctttc
    25441 atgaataaat gctaattctt ctaaagaaag ctttttcgat tcgccctcga tatctgcagc
    25501 ttgcccagca accatacctt gtgatcccgc acaaacagct aattgctgaa gcagcagaag
    25561 cttaggagaa ttacctaagt gtgccatact gattagctgg aatgctcctg taagcaaacc
    25621 atctccggcc aatatagcta atgcttcgcc gaatacttta tgattggtag gttttcctct
    25681 acgaagatca tcattgtcca tcgcaggcaa atcatcgtgg atcaaggaat acgtatgcac
    25741 catttctaaa gcagctgcta cttgatagac gggaacatca ataggctcat caaaggaagc
    25801 aacggccgtt aaaagtaata agggacgtat tcttttcccg cctgctttca ctgaataact
    25861 catcgcatca tagagtcctc tatccgttgt atgctcatct aaaaaagaca agatttcttt
    25921 ttctacagca ggtagatgaa tagctgaaaa gtcagttaat ctcatctcag ctttcctcgt
    25981 ttccgtcaaa atcaacttct tcatttgatt ctgtcatcat tttcgtcaag gtctcttctg
    26041 ctttagctaa tgtatcttgg caatgtttgc ttaattccat tcctcgttta aacgaatcta
    26101 gagctgattc taatggaacg tctccttgtt ctaactgcat gacaattttt tctaattctt
    26161 gcagtgactc ttcaaaagtt ggattttcca tatttttatt ctcctttgtc tatctcttgt
    26221 acttgtcctt taacgattcc atcttgataa tggatcgtca gcaaatcccc ttgatgaatc
    26281 tcggtgacac tttttactac ttgatcattt aacgtcgtat agctgtagcc tcttcccatg
    26341 attttcaatg gactcaacaa atcaagagat tgaacagctt gagcaaaacg ctgctgctgt
    26401 tgtttcatat attgttggat tttctcttct aagcgttttt ctagataagc agtttcttgg
    26461 cgagctgctt taactcgata aagcggagcc acttgttcca attggtggct taatgcgatc
    26521 gtttgttttt ctttcgcatg ataaatttgc tgtgtagctt gatataatcg ctgtttcaac
    26581 tggtctaatt tgatggtctg accttcatac aatctttcag gctgccgaaa tacataggaa
    26641 cgtcgagcac gctcgaaccg ttcttgtttg cgctggatct ggcgtacaaa cgcctgctgc
    26701 aatctagctt gccgttcttg aatgcgcatc aactcttctg acaaaacagg aacagctaat
    26761 tctgcagctg ctgttggagt ggcagcccga acatctgcta ctaaatcagc gatcgtggta
    26821 tctgtttcat gtcctacaga tgaaatcacc ggcgtattag cttgataaat cgcccgagca
    26881 acccgctctt cattaaacgg ccataaatcc tcaatcgaac cacccccacg accgatgatc
    26941 attgtatcga aattgcccat ctcttctact ctctggatat tcctgacaac attgtccgct
    27001 gcttggtttc cttgtacaac tgttgggaac aaaaccaatt ggacaattgg atatcttctt
    27061 ttcaccgtcg tgatgatatc gcggataacc gctccgcttg gactggtaag gaccgcgatt
    27121 cgctttggga aacgtggcag cattttttta ggcccttgaa aaaggccttc cttcatcagc
    27181 ttttcttttc gttcttccaa ttcttgatat aaagcaccta ttccatcagg ctccatgtgc
    27241 tctacataga tttgataaga acctcccgct tcatataatg aaatacgccc aacgacaagg
    27301 actttcattc cttcttttgg ctggaatcgt aatttttgga atgctccttt gaacataata
    27361 gcagatattt ttgcatggtc atcttttaag ctgaaatatt gatgggcatt ggcacgtaaa
    27421 cggaaattcg atatttcgcc tgttaaatat acgcgttcta aataaggatc ggcatcaaat
    27481 tttcttttca aatacttcgt taatgtagtg acagttaaat attcttgtgc catctattca
    27541 cctgctccaa aagcttcggc agattttacg gtttgataca tcagcatcgt gatagtcatt
    27601 ggccctacac cctttggcac aggagtgatc cagcctgcaa taggctccac ttcatcaaat
    27661 ttcacatcac cgatcagctt accgttagca tcacgattca tccctacatc tatcactaca
    27721 gcacctggtt ttacgaattc ttttgtgaca aaatgtccac gtcctattgc tacgactaaa
    27781 atatctgctt cttgtgccag ttcagccaga ttttcggttt tagaatgagc aacagtaaca
    27841 gttgcatctg ccatcaagag tagctgagcc atcggtttac cgacgatatt gcttcgtccg
    27901 ataactactg ctcgtttacc cgccggatcg atagaataag cttcaaacat tttcatgatg
    27961 ccgtatggag tacaaggaat cttgtccggt ttacccgcaa ggagtctgcc taaattcatc
    28021 ggatgaaaac cgtctacgtc tttttcaggg cggatagcta acaggatctt ttcttcatct
    28081 atatgctttg gcaatggtaa ttggaccaat ataccatgaa aactaggatc ttgattgtat
    28141 ttttcaattt ctgcaagtag ttcgtcttca gaaatcgtct caggataacg ctctaccaat
    28201 gaatggattc cgatttcttt tgctacacgt tctttatttc ttacatagat ttggctggct
    28261 ggattttccc ctaccaataa aacgactaat ccgggatgga taccattatc tttcaatttt
    28321 tggatttttt cagcaatctc tgcctgcatt ttttcagcta attcttttcc gttaatcaat
    28381 tctgccatct aaccactccc agatattttt tgtgttttca ttctaccata gaattttccc
    28441 gatttataac aggtttatga tgattcatag aaaaaagcta ggacttacgc cccagcttct
    28501 aattctttca atacattgga taaaacaccg ttgacgaatt tacgtgaacg gtcatcacta
    28561 aatgtttttg ccaactcgat tgcttcattt aaagcaacag ccgctggtac atctgtcaca
    28621 taagtcattt caaaaatcgc gatacgcaag atcaccaaat ccatttttga gatccgattg
    28681 atgtgccaat tgttttttaa atgtttttca atcactttat ccaattcatc tttttttgca
    28741 cagacaccgc caactagcgt atcaagataa acaggaacaa actctgattc atcttcgttg
    28801 atcatgtcgc ggtggtctaa ttcgattgcg ttgaaaatcg catcttcttt tgttaggtct
    28861 gcattgaagt ctaatggaaa taaagcctgc aatgccattt cccgaatctc gtgtcttgat
    28921 aattctttat tcattctctt cctcttcgtc ttcaaaaagt tcttcgattg ttggttgttc
    28981 taatttttca ggaacaacag ctactacatg gatattgact tcagataaat cgatatccgt
    29041 catgaaaagg acttgctggc tgacccgttc ctgcatttcc attgctactt taggaactga
    29101 aacaccatat tctagataac agtaaagatc tactttcaag ccatcttcct cgtttgctaa
    29161 gtaaacacct tttccgtgag ctgcacgtcc taataattca gtgacattgc tggcaaaagt
    29221 tccgcgcatt ccgtatacgc cttctacttt agaagctgca atcccgataa ttacttcgat
    29281 gacttcagga gcgatcacga tctctcctaa atcttggttt gcgtttaaaa tcaaattttt
    29341 ttcatctgcc atttatttat tcctcccttt gcctaagcac gagatacatc cgaattctct
    29401 atgatgcggc atagtgatca cgtggtaaga aaactgccgc cgatcagctg caaactgcta
    29461 tgaattggag aaaacaccca actttattat ctcttacagc ttaaaagttc ttctatagtg
    29521 tatcattaaa tcgagtgatt ttctataaca aagtctgtga acactgattt ttacgcttac
    29581 ttcttctatt gatgaaacta taagatgatc aattcttttg gtgaacgagt caacacacga
    29641 tttccatctt ctgtgatcaa caagtcatcc tcaatacgta ctccaccgat tcctggtaag
    29701 tagatccctg gttcatcagt gatcacattt cctggtacaa attgcttttc tgcacgagaa
    29761 gaaacgtttg gtccttcgtg gatctctaaa ccgatcccat gtcctgtaga atgtccaaat
    29821 gcttccccgt aaccatgttt tgaaatataa tctctagcaa ctgcatctaa ttggacacca
    29881 gtcattcctg gttttgcagc atcaataact gctaattgag catctaagac tatttgataa
    29941 atttctttta atttttcccc aggatcacca atagcgaatg tacgtgtcat atcggaaacg
    30001 tacccttcgt aatagcaacc aaagtccaat gtgatcaagt caccttgttc gatgattttt
    30061 ttgcttgcta caccatgcgg catagccgaa cgcaatccgc tagcaacgat cgtatcaaaa
    30121 gaaacacctg aagcgccaag agaacgcata taaaagtcta gttgatttgc gacttcaatc
    30181 tcagtcatgc ccggttggat cattttcagg atgtgatcat atgccatatc cgcaatatga
    30241 catgcttttt cgataatagc gatttcttct tcatctttca cttctctgag ttcttcgatc
    30301 atctgtgaaa caggtactaa ttccgcatcg atgatttctt ctaaaaccga atattctaaa
    30361 aatgagactg ttgtttcctc aaaagctaat gagtgcagtt cttctttgcg tactaagttt
    30421 gctacctcat caaaaatagg tccgacattt ttaataattt caaacccttg agcctgcgct
    30481 gccgcctgct cggtataacg aaaatcagta ataaaaaatg ctttttccaa agtaataaca
    30541 gctaatcctg tcgtacctgt aaagttcgtt aagtaacgaa gattgtacgg actggtaacc
    30601 aaaaatgaat ccaaattttc ttcttgcatt tttttgcgca atttttctac acgtagcatc
    30661 ataatgaaac tccctctttc tttgcacgct tttttccatt atagcaaact tttcttcgta
    30721 cacgacagtt aaataaaaat ttccggaaaa cttcttttta ttttaaataa ataaagccaa
    30781 aaaggagtca cagtctcgct gcaactcctt ttagtttact attttgtaaa aacttatgat
    30841 cgttttccaa agaaaaatga gacaactgct accaaaatca ctgcgcctaa aatagatgga
    30901 ataagtgcca tacctgcagc ttttggtccc cattcgccga aaatcgcttg tccaatactt
    30961 gaaccgacta gtccagcaat gatattcgtt acacagccca tcgaatttcc tttactggtg
    31021 atcgcacctg caattgctcc gatcacggct ccaacaatca atgtccataa ccaactcata
    31081 atataacctc cttctttgct aatcaatttt agcatatggg atttaaaggg gacaaataat
    31141 tggttttgct atttcatatt ttcttctgca aagtaaaaaa ataaagaaaa cctcctctta
    31201 gaagttttct ttatttttta cttgcgttta aacatactac gaataccacg tttcactaca
    31261 tcaaagaaac tcaatgattg taccattcta tcttcatcta catattctct gatcgcacga
    31321 agcacttttt taggttcttt ggaagaaaat gtgtatgtac catttttttt cgtttgaatg
    31381 gcatatcgag gaatccattt tccctttagc atgactgatg ctagtacata atccacttct
    31441 tcccatggaa tttggatata tttgcgtcta tctcgcgcat ggtaaaactc aaaaccatta
    31501 tcacctatca tgatttgccc gtaatctgtc agacccgtaa acgaagtagc atcaattacc
    31561 aagtctactt ttgtattgat tgattgaacc attttctccc tccagatatt tgtttatgaa
    31621 cagtatacta tataaaaaaa gctcgggaca acgcccgagc tatattttta tggattataa
    31681 aagtccgatg acgtgtccaa caacaccgac tacgaataaa ccaaggatga taacgattgg
    31741 agaaaccttt ttcttaagca accacataca taacaatgtt aatcctaatg cagctaaacc
    31801 aggaatcaat tggtctaagt tgtcttgtaa tgttgttacc ttttcaggag aaagggctaa
    31861 gccattatta acttgttcaa atgcactctt gattccttca ccacctgaag gcaatttatc
    31921 ccattcgatg taagcacctt tatctaattt tacagttgat acaactggta agaatttgat
    31981 tgatacccat ctttgtacaa gggcagctaa tacgaacatc cctaaaattg aagcaccttt
    32041 tgttacgtct tgtaacaatc cgccagaaag gtcatcagta attttagaac cagctttgta
    32101 gccaaattct tgtgtatacc acatgaatgc ccaacgaata atgttccaac ctaagaagaa
    32161 gatgattggt ccaaggatat ttcctcccat agctaatgaa gcacctagtg cacctagcat
    32221 tggacgtact gtaaaccaga acactgggtc accaacacca gctaaaggac ccatcatacc
    32281 tactttaacc ccttgaatcg ctacatcatc aacaggcgcg ccattcgcac gttcttcttc
    32341 cagagccaat gtaaccccta aaattggaga agcaatgtat gggtgtgtat taaagaattc
    32401 caagtgacgt tttaatgctg cagaacgttc ttcttttgtt ttgtataatt ttttgattgc
    32461 tggaatcata gaaaatgccc agccaccgtt ttgcatacgt tcgtagttcc atgaaccttg
    32521 aatgaaggta gagcgccacg caacagctaa acgatctttt tttgataatt tgattttttc
    32581 ttctgccatt ttattttcct ccttcaagat taatagtcat tcaaaatgtc gcctaaaggg
    32641 tcaccagagt tgcttccgcc tccattattt gatgaaccac ccattttaga aaggttcaag
    32701 taaataagag ctaatgcaac acctaaagca cctagagcaa tcaatgttaa ttgagaaatt
    32761 gcagctacaa cgaaaccaat aacgaagaat ggccatactt cttttgtagc catcatgttg
    32821 attaccaaag cgtaacctac agctacgacc attcccccac cgatagccat accatctgtt
    32881 aaccatgctg gcattgattc aaggaatgat tgcacagttt gtgctgggat aaatagcaac
    32941 gctgctgctg ggaccgcgat acgaacccct tgtaaacata cagcaatgat gtgccacatt
    33001 tcaaccttac ggatgtttcc ttcttcagca gccgcatcca tcaaatgaac gattggaaca
    33061 gcgattgtac gtacgatcat tgttaagaac aaccctgcaa cagccaaagg aacggcaatc
    33121 gcgatcgcag aaggaacacc tttaacacct tgtccaccta aaactaaaat aattgctgat
    33181 gctactgatg ctaatgcagc atctggtgct acagcggcac cgatatttgc ccaacctaaa
    33241 gcgatcatct gaagcgtacc gccaagtacg ataccagctt cgaggttacc ggtaactaaa
    33301 ccgatcaatg tacatgccac tagtggttga tgaaattgaa attcatcaag aataccttcc
    33361 ataccagcta gaaaggcaac taatacgact aaaattattg aaataataga catgataagc
    33421 ctcctataaa ttttaatatt atttattacg cttttgctaa ttcagctttt gcttttttca
    33481 aaatatcatc catgttatcg cgtgaatcat taggtacttt acgtacatcg aatttcacac
    33541 ctttttgttc aagcttctca aacgcttcaa cgtcttcttg tcccatagac aatactttac
    33601 ttacaacgac tttaccgacc gaatgcgcca ttgacccaac atttaattct ttgatgtcta
    33661 cgccaccttc aacagctgtc aatacgtctt caggattttc aaataaaagc aatgctttgg
    33721 tatttccaaa acgagggtct tttgctactt cgatcatttt gctaatagga ataacatttg
    33781 cttttacacc tggaggagca gcttgttcga ttagtctctt acgcagatca tcctttgata
    33841 ctgcatctga aacaacgata atacggtttg gttgcactgc ttttgtccaa gctgtcgcaa
    33901 cttgtccatg taacagacgt gagtcaacac gtgctaatac atatttgatt tttccgtctc
    33961 cgaccacagt tccttctgga agtgctcctt taggttgtgc gtcttcaact gctgctttcg
    34021 gtgcttctgc cggttctaat tcttccggtt ttacttttac accgtctttt gctgtttcaa
    34081 tgatgtgtgc tgcaatttca tgagcagatt ccattgaaaa gcgtgatgcg taggcttcaa
    34141 tcaacattgg taaattcaaa ccgctgacga ttgcccattt atctttgtgc tcttcgaata
    34201 atgtgtttga ttgattgaac ggtgttccgc cccataaatc aactaagaat aacacttcgt
    34261 cttgattatc aaaagaagca accgcttctt ccaatttggc tctcaagtca tctggacctt
    34321 cacttggttt caaaatcacg gctttgacgt tttcttgttc gccaaagatc attgaaccgg
    34381 attgtaagat tccttcagca aattgtccat gacttgctag gataatccct accatattta
    34441 tacctcctat ttacttttgt tgtaacaaat tcaaaaatat taataattgc taaccattac
    34501 aacagaatta tttgaccttt tccaacaacc attcttgcta cacctttttg attttcgcta
    34561 gtagttccat caaaaggatt ttcctgtcag caggtacttt tcgaatctct aactccactt
    34621 cttgactgtt caagtaacga aaggaatcaa tatcttgttg atttactgaa acaaaattgg
    34681 taatcattga tttgccttca gtaaaactca taccgccgat attcaacgac ttcaactgta
    34741 caccgcagcg gacaaccgct tcaacatctt ggggattggt gaataaaagc atcacttttg
    34801 ctcgtgataa taatccacta tgatagattt gaatcatttt ctgcacggtt attacatttg
    34861 ctgtgatccc cggcggtgca gcttctttta gtagaaactt tctcaattta tcttgagcga
    34921 ctacatcgct gatcacaatg atacgttcaa cacctgtttg cttcgaccaa actgttgcga
    34981 cttgcccgtg gattaaacga tcatctatac gtgccaatcg aatatccatc tgcaaaactc
    35041 cttcttcctc actacttcac agaaacccct atcatttctg tgtgaacaac cacctcctag
    35101 cacacactac tataagcaaa aagcgtgcca acttttttac gatacactaa ttgaatttaa
    35161 aggattctta accaatacac aaaaacgata cactataaaa gtgtatcgtt tttgtgtatt
    35221 catggcacag tgtattcttt tttatctaac tgattatgga ctagacgtag cagataatac
    35281 atttcttcta tcggcacttt tacttttatc tgatcttcca acagaacaac tgaggctgcc
    35341 aattggctat aaagaggatc attcgttgta tttgtcaatt cgttttcttc cacagtcaat
    35401 ggttcattcc gaataatacg ttcgatcatt cctgctgtgt gcatcactag gttgattttg
    35461 aatccatatt cttcatcgcc gattcccgtc gtctgagaga tttgtgtaga aaattgccac
    35521 attggatcga ttaacttact accattaata aagatgaagt tatcagagat aaactcaaca
    35581 cacgtatttt tagctgactc ttcatctagg gagatgaatt cagtttcctc caattctgat
    35641 tccatcagca actgatccaa aatgacttcg atattttgat caataaaacg ttctaaagga
    35701 ataaatgggg cttggatatt caaatcagta atcccagtag tagctatgat ctgatattct
    35761 ttttggataa ctggcagttc ttctttcact tccacaatcg acaaagctag aacttctaat
    35821 tctgtttctt gacgcttgtt taccgctcgt tcgatcaatt ctttgattct ttgtgctgta
    35881 ccttcgccag atgcgcagat agccaaaaca gcttttttct tatgaatagg ttttgcttcc
    35941 tgaacagatt ctacagaaga tatttctgcg taacctcgaa aatttttcaa tgattcatgc
    36001 aacatatcta gatctgtccc taatactgag acctttcgta cagtttccaa aactagagaa
    36061 gttgttacca tatcaacagt acgtactgga attccagtgt ctctttgaat ttgcgaatta
    36121 aacgacgcaa gggaccccat atctactaag agtatgaccc cgcttccttc atctacttct
    36181 tgtacattgc gttcaattcg gtccaatgct gttttcggat ccatatccaa aggcatgtct
    36241 actgctcgta cgttctctgc atctaacaac tgttggacaa cttggaccat actgcttgct
    36301 gtgctgtttc cgtgagctgc aacgactaca ccgattctgc cgcttgcttg atttgcccgt
    36361 aacgagacga gtagaacggt caagtaatag tcttcacttt ctggaatctc cacttgaaaa
    36421 tacgtttcga tataactacg cacttctttc gcaacagcat attcatctgg aaaatcaaaa
    36481 gccatctcac ggatgttatc attcgtatgc cgttcttctc ctaagttgat ttttttcaaa
    36541 aaagaactga tatgcaagct catagcataa ataaagtttt gctgaaactc atatggtaaa
    36601 gatcttttga ccattacata aatctgattg gtcacttcta tgattttcgg atctacaaat
    36661 tctgctaatt tgctgtctgc attaaaagaa aaaccatggt ctttatagaa agacttcaga
    36721 tggatgttga tatctgtaga aatgaaatga ttgattgctt cttgatccaa accatccgac
    36781 ttcagtaaag ctgctttatc tccgatgata tcatatagat tataaggtaa ctcataggag
    36841 tccgattgga ttttcatgat cgtatcattt ggtgctacag ttatctttgg ctccaatatt
    36901 ttagaaagct ctgccattgc actgcggttg cttgctaatt gaatcaatcc gctacgaatc
    36961 ccttctgtca aatcatcgat tgtgatcgag atttcgggtg attgcatatg atttagaaat
    37021 cctcgtgcac atactaactg gatatttgac ttcaattgtc ctatatttcc ataggtcaca
    37081 ctgccgatca atgcttttgc tacatcttct gtcaaagaaa tctttcgctg cgtgcggttt
    37141 gcttcgtggg caatcatgat ttttactaaa tcgactttct catttgcagg acgtttttca
    37201 aaagccggaa gtttgatatt gattgggata cgccgaacaa aagtttctaa tagagaagaa
    37261 cctgggtctt ctgtggtcgc acctacgatc cgtacatttg cttcatggga tttcgttgtt
    37321 tccccaagac gactgtaagt tccatgatcc ataaagtaaa agatcatttc ttgaccttca
    37381 ggcggcaacc ggtgaatctc atccaaaaac agcataccgc catccgcttg atcgataatc
    37441 cccatttttt cttcatctgc gcctgtaaaa gcaccttttg cgtacccaaa taaatgactc
    37501 atcaaaagct cagggttatt ggcataatca gcacaattga aaacaatcag ttccgtttcc
    37561 tcatctatta catgatgcgc ttttgcaaaa tgatacatcg cgtgtgcaaa ataagtcttt
    37621 cctgatccgg taggtcctgt aatcaaacaa tttaatcctc ttggcggata caaaatcgca
    37681 gcttttgcct gttccactga atttttcatg ctgccgttag ctccaatgat ttttgcaaag
    37741 atatccttag tatctatttg gagtgtattt ttttcttcag agaccggcgt ttctttgtaa
    37801 ctgactacat gttttgtaac tggctttacg tgcgtaacga gtgtttgata tatgtaacgt
    37861 actggtcgtc cgtctgtctt tatcagtttt ccttcgcgca ctagctgatt cagatcttta
    37921 ctgctatttg ttcgctgaat ccctaacact tcagcaattt cctgtgttgt tactccttga
    37981 tcatattctg cctttgttaa atgagcggtt ttttctttga catacatata aattcggtca
    38041 atgcgttttg ccaatggtat cgctcctttt ctatagacta ttgtacttag gcttcaaaac
    38101 ataatactat gtatttattt ctgtttcgtc aactatttac ctgattttgt tttgttgata
    38161 acacgcggat agatagaaat gttcaattta aaagaacgaa aaatcccgat acgattttta
    38221 taatggaaac aagaaaaaga acactgttta ccatataatt atttcactga tagcaaacaa
    38281 tatagcttcc atcaattgaa aacgatagtt atcaaaacat cctgattcca aattggcaaa
    38341 aaaatattaa gtgtaaaaag acaaagtaaa cttcgtagcc tccactacaa cttttatttt
    38401 tataaacaaa aaaggctgtg acaaaagtct tgtcacaacc tctttaatca taagattcaa
    38461 ttaagcttct tgagctactg gataaacaga cacttgtttt ttgttacggc ctttacgttc
    38521 gaaacgtact acgccatcaa cttttgcaaa taaagtgtcg tctccaccga tacccacgtt
    38581 tacacctgga tagatttttg taccgcgttg acggtaaaga attgatccac ctgtaactgt
    38641 ttgtccatca gcacgtttag cacctagacg ttttgattct gagtcacggc cgttagatgt
    38701 agaaccgcca ccttttttgt gggcaaataa ttgtagattc atagataaca acatggtctg
    38761 cacctcctat ttatcattaa ttgttttaat ttggatatat tctgagtttt cagcttctat
    38821 cgattgtaat cctaataaaa gattttctaa tagaatctca gcaatattgt tttgttcttg
    38881 tgtcattcct gaagtgactt cggtataaag ataaccgcct tcttcttcat tcatttcaac
    38941 aatcggctca aaaccggcta atgaggcaat cccgttgaca gtgctgatcg ctaacgcaga
    39001 aacacccgca caaacgatat cacttccata aggaccagca tcagcatgcc cggtcaaagt
    39061 gaatgaaacg atctgacctg cgtcatttcg cttaaatgtt cctttgatca tatataggcc
    39121 ttctttctca aacgctatta agcgttgatc gcgtcgataa ccacttttgt atatggttga
    39181 cgatgacctt gtttacggtg tgaatgtttt ttaggtttgt atttgaaagt aacgactttc
    39241 ttttgtttgc cgtgtttttc aacagtacct tcaacagttg cacctgcaac agttggagcg
    39301 cctacttttg tagattctcc gcctactaag ataacttcgt caaaaacaac tttttctcct
    39361 gcttcaacgt caagtttttc aacatagatt gcttgtccca cttcaacttt tacttgttta
    39421 ccaccagttt taataattgc gtacatgcta taaagcacct ccttctgata tacttagact
    39481 cgccatccga agtgacagca tatgctgtgc ttattacttg ttctgagcgg ttgtagctgt
    39541 ggagtccaca attacaacag tactagctta ccagaacagc aaccgttagt caactaaatt
    39601 taaaatcaaa aagtaaaaca aaaattcaag caatacttct gcttttctat cctcacagtt
    39661 ctaccaaata aaatagtcgc attgtgtttc ttttattctt ataataaaag aaaaaggagg
    39721 agcataaatg gaatacccaa aagaactgat acaagaagct tctttaagaa tgaacggccg
    39781 tcctgtagaa ggatttatcg ctgggcaagg acctcttcat cctaaactga tgctcgtggg
    39841 agaagcccca gggaaaacag agattgatac gcatgttcct tttagcggtc aagctggaaa
    39901 agaattgatg caggcacttt cttcaactgg cttaacacga gaagaggtgt atatcaccag
    39961 tgctgtaagg agccgcccgt atcgcgtgac tcatcggatc aataaacgga cacaacagtc
    40021 agaaactgtt tatccaaatc ggacacctac tcgttccgaa gtatttgcgc atgcaccaat
    40081 attggattac gaactgatga aggtccagcc aaaattaatt gcgacacttg gaaacattgg
    40141 tcttcaacgt ttacttggca aagaatatac ggttaccaaa aatcatggtc aactctatac
    40201 agggcctgtc ttacaattaa cagaacaaaa ggatgcctac gaatggtcag aaaaaaacta
    40261 tcgaattctt ccactttttc atccagcagc tattttctac aatcgctccc ttgtacctgt
    40321 tatccatcaa gactggcaag tactagcttc acttttacag caatattaaa aagaacagac
    40381 aagaccataa aatcttcagt ttacagtctt gtctgttctt tttcattgtt ttaatggcta
    40441 attgcagtat atttcgcatg taaagctgtc aacgcatgag caactgtttg atgttgatgc
    40501 gtgaatgtat gttcccacaa gtgagaaagt tgtttttcta cttctggctg caaggaagta
    40561 tagataatcg tttgtcctct ttgctctgct ttttcttgaa attcatcaaa aactgcacgt
    40621 gccgtcaaat caatacttgg tacgcctcgc atggagaaaa tgacaccatc gtgatgattt
    40681 agttcttcca atttattttt gagtttttct gaagacataa aaaacagtgg accactaata
    40741 tacactactg tccagttatt cagcttttcc gtttctggca gccccattct ttgccaatcg
    40801 atcgtttcta atgaaatcgt gatagcagcg cttttgacaa caaaaaatac acagccgccg
    40861 atcaccccga tcacgatagc aatacttaaa tcaaagataa ctgtacaaat catcgtaagg
    40921 ataaacaata gaactgctga ccaatattta ttagtaaata gctccttgat cgtttcccat
    40981 tcattcatac gccaagccgt cacaatcaaa acacctgcaa gtgctggcat tggaatctgt
    41041 gacatcactg gcgcaaaaac aagcatggag agcagtaaga caactgcatg tatcatacca
    41101 gctaatctag tttgtgcacc agatttgatg gccacgcttg tacgggcgat ggccgcagta
    41161 gcaggaatcc cgccaaagaa aggaagcaac agatttccaa ttccttgcgc cattaattct
    41221 tgattgctat ctaagggctt atttgccatt cggccagccg aagcaccaca tagcaagctt
    41281 tcaatcattc ctaataaagc aatgctaata gctggaactg ccacttgttg caaggcagaa
    41341 aagctaaaat ctcccatatt caaacgatta ctactgatca atgtttgtgg aatcttaccc
    41401 acagttgcta ctggtaaatg gaacaatagc ataactgctg ttgccaaaat gatagccagc
    41461 agcgaactag gcattcgttt cgcccatttt tttggataga taaacatccc taaaatgact
    41521 agccctccca tgatcaaaga ggctgtgttt atcggaaatc ctatcgtttg ataactactt
    41581 agtttttcaa taacgtttgt accttccgag tgaacaccaa ataaattatc gatctgacct
    41641 aatgcaataa tgatagcaat acctgaagta aaccccgtaa ttacgggtgc tgggataaaa
    41701 gaagtcagtg ttcctaatcg gaaaagtcct gctgcaagta aaaatagacc cgctaaaaaa
    41761 gtagcaagca atacaccttg cattcccttt gttgccgcaa tagacatcaa gatagctgcc
    41821 atcgcacctg tcggacctga tatttgataa aaaccgcctg ataaaccgcc aataacgata
    41881 ccggcaatga tagccgtaat caaacctgcg gccgcatcag ctccgcttga taccccaaaa
    41941 gccaaggcta aaggcaatgc tacagcagct actgtgatcc cagccaaaag atctttctga
    42001 aatcttgaaa gatcataacc tgaaaattct ttttttagtg aggaaacata atttttgaac
    42061 acctatctaa ctcctttttc atgaaatttt ctaa
//
LOCUS       DAPCMU010000008        41407 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPCMU010000008 DAPCMU010000000
VERSION     DAPCMU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.008"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..1256)
                     /locus_tag="RD744_000360"
     CDS             complement(<1..1256)
                     /locus_tag="RD744_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556394.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLK"
     gene            complement(1267..1935)
                     /locus_tag="RD744_000361"
     CDS             complement(1267..1935)
                     /locus_tag="RD744_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDU0556395.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2065..2649)
                     /locus_tag="RD744_000362"
     CDS             complement(2065..2649)
                     /locus_tag="RD744_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0556396.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2980..3213)
                     /locus_tag="RD744_000363"
     CDS             complement(2980..3213)
                     /locus_tag="RD744_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDU0556397.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3228..4178)
                     /locus_tag="RD744_000364"
     CDS             complement(3228..4178)
                     /locus_tag="RD744_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDU0556398.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4175..5017)
                     /locus_tag="RD744_000365"
     CDS             complement(4175..5017)
                     /locus_tag="RD744_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDU0556399.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5019..5738)
                     /locus_tag="RD744_000366"
     CDS             complement(5019..5738)
                     /locus_tag="RD744_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDU0556400.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6430..6588
                     /locus_tag="RD744_000367"
     CDS             6430..6588
                     /locus_tag="RD744_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556401.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6660..7886
                     /locus_tag="RD744_000368"
     CDS             6660..7886
                     /locus_tag="RD744_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0556402.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7974..8366)
                     /gene="rpsI"
                     /locus_tag="RD744_000369"
     CDS             complement(7974..8366)
                     /gene="rpsI"
                     /locus_tag="RD744_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDU0556403.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8382..8825)
                     /gene="rplM"
                     /locus_tag="RD744_000370"
     CDS             complement(8382..8825)
                     /gene="rplM"
                     /locus_tag="RD744_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDU0556404.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9344..9811)
                     /locus_tag="RD744_000371"
     CDS             complement(9344..9811)
                     /locus_tag="RD744_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDU0556405.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9931..11469)
                     /locus_tag="RD744_000372"
     CDS             complement(9931..11469)
                     /locus_tag="RD744_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDU0556406.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11549..12235
                     /locus_tag="RD744_000373"
     CDS             11549..12235
                     /locus_tag="RD744_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDU0556407.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12436..12963)
                     /gene="msrA"
                     /locus_tag="RD744_000374"
     CDS             complement(12436..12963)
                     /gene="msrA"
                     /locus_tag="RD744_000374"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDU0556408.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13047..13724)
                     /gene="pgmB"
                     /locus_tag="RD744_000375"
     CDS             complement(13047..13724)
                     /gene="pgmB"
                     /locus_tag="RD744_000375"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDU0556409.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13721..14851)
                     /locus_tag="RD744_000376"
     CDS             complement(13721..14851)
                     /locus_tag="RD744_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDU0556410.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14851..17034)
                     /locus_tag="RD744_000377"
     CDS             complement(14851..17034)
                     /locus_tag="RD744_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDU0556411.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17031..18056)
                     /locus_tag="RD744_000378"
     CDS             complement(17031..18056)
                     /locus_tag="RD744_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0556412.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18101..18928)
                     /locus_tag="RD744_000379"
     CDS             complement(18101..18928)
                     /locus_tag="RD744_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDU0556413.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18925..19992)
                     /locus_tag="RD744_000380"
     CDS             complement(18925..19992)
                     /locus_tag="RD744_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDU0556414.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20018..20854)
                     /locus_tag="RD744_000381"
     CDS             complement(20018..20854)
                     /locus_tag="RD744_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0556415.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20851..21720)
                     /locus_tag="RD744_000382"
     CDS             complement(20851..21720)
                     /locus_tag="RD744_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0556416.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21790..23073)
                     /locus_tag="RD744_000383"
     CDS             complement(21790..23073)
                     /locus_tag="RD744_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDU0556417.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23087..24772)
                     /locus_tag="RD744_000384"
     CDS             complement(23087..24772)
                     /locus_tag="RD744_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDU0556418.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25047..26072
                     /locus_tag="RD744_000385"
     CDS             25047..26072
                     /locus_tag="RD744_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0556419.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26667..30341)
                     /gene="rpoC"
                     /locus_tag="RD744_000386"
     CDS             complement(26667..30341)
                     /gene="rpoC"
                     /locus_tag="RD744_000386"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDU0556420.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(30399..34016)
                     /gene="rpoB"
                     /locus_tag="RD744_000387"
     CDS             complement(30399..34016)
                     /gene="rpoB"
                     /locus_tag="RD744_000387"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDU0556421.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMYQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34744..35712
                     /locus_tag="RD744_000388"
     CDS             34744..35712
                     /locus_tag="RD744_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0556422.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35730..36635
                     /locus_tag="RD744_000389"
     CDS             35730..36635
                     /locus_tag="RD744_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556423.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36632..37438
                     /locus_tag="RD744_000390"
     CDS             36632..37438
                     /locus_tag="RD744_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0556424.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37574..38563
                     /locus_tag="RD744_000391"
     CDS             37574..38563
                     /locus_tag="RD744_000391"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDU0556425.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38880..39404)
                     /locus_tag="RD744_000392"
     CDS             complement(38880..39404)
                     /locus_tag="RD744_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDU0556426.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39691..41388
                     /gene="scm"
                     /locus_tag="RD744_000393"
     CDS             39691..41388
                     /gene="scm"
                     /locus_tag="RD744_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDU0556427.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT        12237 a         8573 c         7348 g        13249 t
ORIGIN      
        1 tcttttaagt ctttaataag gtcagcaatc ctttccatat taatataatc caacccactt
       61 gtaggttcat caaatagaaa taaattagtt tttgacaatg aagctataga aaccaataat
      121 ctttgttttt ctccaccgga taaatcaaac ggtaattgat ctttcatttt ataaagttcc
      181 atttttttta aaattttttc ttgctgatct ttactaacta tttcattccc cagggctaac
      241 tcgctcctca ctgattctgt gaaaaattgg taatccattt cttgcattac taaaaacggc
      301 gatgacaaat tttttttatt ttcgattatc tttcctttgg tagggcgaat aatcttcatg
      361 agtattctta gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc aataacatct
      421 ccaactttga catcaaaatt tatgtctttt agaatatttt cataagtaat ttcttcaact
      481 tttaatacat ctgcacttac cttttcgata tagggaactt caatcgaact attaaaaatg
      541 tcaaatttcc ttaatgcgtt attgttttca gtttttaaca actgaatagt tgtagttttt
      601 acagttctat ttttaacaaa tagaacttga tcaattaatt ctttcaaata gtaaaaacga
      661 tgttcagcta taacaatagt aaatccttta ttttttaaat cacaaagtaa gtcagcaagt
      721 ttcttagtcg ctttgaagtc caaatttgaa gacggttcat ccaatataat taatttagag
      781 tttagcgata aactacatcc aatagaaatt ttctgccgtt caccacttga tagcgaaaaa
      841 atgtttttat ctaatatatt tgaaagatct aataattcag ctaattcatt gattctttta
      901 tccatatgct cttttgtaaa tccaaagttt tccatcgaaa atactaactc actcgttgta
      961 tttgttgtaa agaattgtcc tctaggatct tgacttacgt agccaatatt ttttgatata
     1021 ctttcttgtg ttaaggattg tagcgagtta ttttcaaata aaatttcccc aaccaatttt
     1081 ccgtcataaa gtcttggaat aagtccattc attaacttta atagtgtgga tttcccactc
     1141 ccagaagaac cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca
     1201 agcgcttgtt ccttaacatc cttatatgaa aaagaaacat tgttaaattc aaacaattct
     1261 ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc
     1321 taataagctc atgtttgttt tatagtaaga acttcttttc acaggagatt ctatcccctt
     1381 tgttattgca ttagcggtca tttcttcagc aatcatagtt gcacgtataa gaacgggaac
     1441 taacagaaat tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt
     1501 taaagattct ttaatcattc ttatttcaga acgataagtc ggaaagaatc tgatcactac
     1561 tgtaatactt aacattattt ttttcggtaa atgtattttt tctaaagagg ataataattc
     1621 attgctagta tagacactaa ccaacaacca tgagatcatt acgaccggca acattcgaaa
     1681 tacaatatat aaggatgtcg caaaaaatat agtaaaaaac tctatcttga tagcatcaat
     1741 aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac atgtctttat
     1801 tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt gaaatatatt
     1861 tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg
     1921 attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga
     1981 taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca
     2041 attctcaatg caagcttttt ttaattaact cagtaattca atccacccag gaacagaaaa
     2101 ttgatacaat cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat
     2161 atcatagaat gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac
     2221 taaattttga ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt
     2281 cgtcacaaat gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa
     2341 taaaatcaaa tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc
     2401 attgaagttc tctctccaac ttttctctat atctatattt tctaagttta agaggtattt
     2461 ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc
     2521 aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt
     2581 caggcacagt tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc
     2641 actttctatt aacttttggt gagttataat ataatctctg gtttctatgg gtacttcctc
     2701 ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt
     2761 ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca
     2821 aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat
     2881 ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc
     2941 caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta
     3001 atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc
     3061 atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc
     3121 taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata
     3181 aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg
     3241 ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt
     3301 cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt
     3361 gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt
     3421 gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg
     3481 ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat
     3541 ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct
     3601 tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt
     3661 ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg
     3721 ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag
     3781 taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca
     3841 tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa
     3901 aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg
     3961 ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg
     4021 gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact
     4081 ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc
     4141 cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta
     4201 aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa
     4261 caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg
     4321 aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat
     4381 atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc acactttgca
     4441 tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat
     4501 tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa
     4561 atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat
     4621 cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa
     4681 ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat
     4741 ctccttttat tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa
     4801 atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca
     4861 aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac
     4921 ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt
     4981 gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat
     5041 atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg
     5101 tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt
     5161 aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc
     5221 ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa
     5281 tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat
     5341 tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact
     5401 ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata
     5461 gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca
     5521 tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac ctctccctga
     5581 tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca
     5641 ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa
     5701 tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata
     5761 atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata
     5821 gtttagattt ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta
     5881 tagtaagtag ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta
     5941 aaactacttt attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt
     6001 tgataagcat caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat
     6061 atgaaaatca gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat
     6121 cgttattaat agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt
     6181 ttgaataacg atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg
     6241 acaaatactt agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca
     6301 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
     6361 tacccaaagc aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc
     6421 agagtctacc tgaataaaaa acaaacctgt gcatttctag gaatcgataa taatactcta
     6481 gataagtgga ttcgtatata taatttccca gtaataagag ttggtggtgt agttcgcttt
     6541 gacgtagaag atattaaaga ctttatgaat acatttaaaa taacttgaaa ttttcttctc
     6601 gccgcatata ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt
     6661 tgcctacaaa gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta
     6721 aagaaataac aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg
     6781 cgattaaagc cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg
     6841 caaactcatt agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc
     6901 ttcctagata tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca
     6961 ttagtaatac aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa
     7021 tgaattatct aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag
     7081 aatatgcaaa tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa
     7141 tactaaatta tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta
     7201 aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag
     7261 aacttactaa ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc
     7321 atgattacac actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg
     7381 ctttgcaatg gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag
     7441 ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag
     7501 aagttgtaat gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat
     7561 taaaaattaa gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag
     7621 taaattgtcc tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc
     7681 atcctgactt agttcattta tctccacaca aactccgtca tacttatgca actatagcta
     7741 gacaaggtgg agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta
     7801 caacaaagat atatgtgaac acaccagata tagttgataa ggctgttttt gaagcttttc
     7861 agagaggatt gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag
     7921 aagcactttc caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt
     7981 tttgagaatt gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata
     8041 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
     8101 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
     8161 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
     8221 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
     8281 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
     8341 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
     8401 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
     8461 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
     8521 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
     8581 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
     8641 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
     8701 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
     8761 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
     8821 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
     8881 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
     8941 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
     9001 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
     9061 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
     9121 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
     9181 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
     9241 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
     9301 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
     9361 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
     9421 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
     9481 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
     9541 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
     9601 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
     9661 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
     9721 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
     9781 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
     9841 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
     9901 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
     9961 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
    10021 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
    10081 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
    10141 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
    10201 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
    10261 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
    10321 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
    10381 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
    10441 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
    10501 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
    10561 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
    10621 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
    10681 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
    10741 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
    10801 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
    10861 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
    10921 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
    10981 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
    11041 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
    11101 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
    11161 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
    11221 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
    11281 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
    11341 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
    11401 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
    11461 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
    11521 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
    11581 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
    11641 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
    11701 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
    11761 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
    11821 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
    11881 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
    11941 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
    12001 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
    12061 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
    12121 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
    12181 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
    12241 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
    12301 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
    12361 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
    12421 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
    12481 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
    12541 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
    12601 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
    12661 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
    12721 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
    12781 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
    12841 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
    12901 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
    12961 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
    13021 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
    13081 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
    13141 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
    13201 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
    13261 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
    13321 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
    13381 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
    13441 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
    13501 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
    13561 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
    13621 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
    13681 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
    13741 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
    13801 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
    13861 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
    13921 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
    13981 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact
    14041 ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca
    14101 gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa
    14161 atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc
    14221 ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat
    14281 cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg
    14341 tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa
    14401 aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa
    14461 cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc
    14521 ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga
    14581 tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt
    14641 tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg
    14701 atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa
    14761 aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt
    14821 tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc
    14881 tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt
    14941 ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa
    15001 aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt
    15061 cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc
    15121 agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc
    15181 ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat
    15241 cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga
    15301 aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg
    15361 cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct
    15421 gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc
    15481 ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg
    15541 acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata
    15601 atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc
    15661 aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc
    15721 tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc
    15781 ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc
    15841 tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc
    15901 ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct
    15961 tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac
    16021 ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg
    16081 ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg
    16141 gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga
    16201 atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg
    16261 ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg
    16321 gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt
    16381 ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc
    16441 tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga
    16501 tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc
    16561 aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa
    16621 cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag caacacgaca
    16681 agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc
    16741 tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa
    16801 atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc
    16861 tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt
    16921 ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg
    16981 actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta
    17041 ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat
    17101 ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga
    17161 gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt
    17221 ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa
    17281 aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg
    17341 atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt
    17401 ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac
    17461 gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga
    17521 atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag
    17581 cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca
    17641 tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc
    17701 aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata
    17761 gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg
    17821 aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt
    17881 tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc
    17941 tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa
    18001 gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat
    18061 ttctctctcc tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat
    18121 ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt
    18181 tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt
    18241 gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat
    18301 gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa
    18361 tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt
    18421 gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc
    18481 ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac
    18541 gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg
    18601 gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct
    18661 cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga
    18721 taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt
    18781 ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt
    18841 attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa
    18901 atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt
    18961 gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa
    19021 tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca
    19081 acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga
    19141 tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc
    19201 agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctat
    19261 taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg
    19321 tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta
    19381 atcggcaccg tgtttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat
    19441 ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc
    19501 gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa
    19561 ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag
    19621 tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac
    19681 taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat
    19741 catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg
    19801 caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa
    19861 gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt
    19921 cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac
    19981 taattttttc atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga
    20041 ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg
    20101 tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt
    20161 tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat
    20221 aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca
    20281 atggtataat gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt
    20341 cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa
    20401 gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg
    20461 aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa
    20521 agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc
    20581 tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga
    20641 aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc
    20701 attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg
    20761 tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt
    20821 aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag
    20881 caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag
    20941 atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt
    21001 cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt
    21061 tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc
    21121 accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa
    21181 tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct
    21241 agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa
    21301 cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga
    21361 aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa
    21421 cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa
    21481 gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc
    21541 aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa
    21601 aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat
    21661 gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat
    21721 tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag
    21781 aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt
    21841 tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat
    21901 tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc
    21961 aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag
    22021 ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc
    22081 catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc
    22141 gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg
    22201 tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat
    22261 gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt
    22321 cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc
    22381 tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt
    22441 tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc
    22501 aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc
    22561 ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc
    22621 ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag
    22681 gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc
    22741 cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat
    22801 tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc
    22861 ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag
    22921 ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt
    22981 ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc
    23041 tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat tttattattt
    23101 tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa
    23161 tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat
    23221 tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa
    23281 aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt
    23341 ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct
    23401 gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc
    23461 ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga atataaattg
    23521 ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc
    23581 attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg
    23641 gactggtcgt tccatctggg tttttcttgt agtttactcg ggcaccctct ttcttcagct
    23701 gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga
    23761 taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg
    23821 tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag
    23881 gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca aaataactga
    23941 tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat
    24001 cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag
    24061 ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt
    24121 ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca
    24181 agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa
    24241 tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca
    24301 gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc
    24361 aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc
    24421 tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa
    24481 cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg
    24541 tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt
    24601 caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc
    24661 ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt
    24721 tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc
    24781 cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc
    24841 aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta
    24901 tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta
    24961 aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg
    25021 ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag
    25081 ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata
    25141 aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg
    25201 caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc
    25261 atacgaccta tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg
    25321 aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa
    25381 aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga
    25441 tggatgaaac acttcttttt gaattggatc aatataatat tcctattgtg gtaatcggaa
    25501 aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc caggatagtt
    25561 acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc
    25621 cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc
    25681 accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca
    25741 tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg
    25801 atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg
    25861 atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt
    25921 tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc
    25981 aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc
    26041 tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact
    26101 gaggataagt cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg
    26161 gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga taactataat
    26221 gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta
    26281 taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg
    26341 gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat
    26401 tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat
    26461 caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg
    26521 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
    26581 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
    26641 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
    26701 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
    26761 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
    26821 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
    26881 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
    26941 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
    27001 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
    27061 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
    27121 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
    27181 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
    27241 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
    27301 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
    27361 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
    27421 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
    27481 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
    27541 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
    27601 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
    27661 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
    27721 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
    27781 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
    27841 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
    27901 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
    27961 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
    28021 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
    28081 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
    28141 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
    28201 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
    28261 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
    28321 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
    28381 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
    28441 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
    28501 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
    28561 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
    28621 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
    28681 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
    28741 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
    28801 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact
    28861 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
    28921 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
    28981 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
    29041 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
    29101 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
    29161 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
    29221 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
    29281 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
    29341 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
    29401 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
    29461 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
    29521 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
    29581 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
    29641 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
    29701 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
    29761 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
    29821 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
    29881 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
    29941 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
    30001 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
    30061 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
    30121 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
    30181 aacaaccctt cacgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
    30241 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
    30301 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
    30361 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
    30421 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
    30481 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
    30541 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
    30601 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
    30661 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
    30721 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
    30781 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
    30841 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
    30901 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
    30961 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
    31021 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
    31081 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
    31141 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
    31201 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
    31261 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
    31321 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
    31381 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
    31441 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
    31501 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
    31561 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
    31621 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
    31681 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
    31741 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
    31801 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
    31861 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
    31921 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
    31981 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
    32041 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
    32101 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
    32161 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
    32221 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
    32281 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
    32341 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
    32401 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
    32461 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
    32521 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
    32581 tttgtttgat acatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
    32641 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
    32701 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
    32761 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
    32821 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
    32881 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
    32941 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
    33001 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
    33061 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
    33121 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
    33181 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
    33241 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
    33301 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
    33361 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
    33421 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
    33481 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
    33541 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
    33601 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
    33661 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
    33721 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
    33781 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
    33841 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
    33901 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
    33961 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
    34021 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
    34081 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
    34141 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
    34201 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
    34261 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
    34321 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
    34381 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
    34441 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
    34501 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
    34561 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
    34621 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
    34681 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
    34741 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
    34801 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
    34861 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
    34921 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
    34981 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
    35041 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
    35101 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
    35161 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
    35221 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
    35281 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
    35341 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
    35401 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
    35461 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
    35521 ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca
    35581 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
    35641 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
    35701 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
    35761 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
    35821 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
    35881 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
    35941 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
    36001 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
    36061 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
    36121 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
    36181 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
    36241 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
    36301 ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc tttgctgatt
    36361 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
    36421 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
    36481 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    36541 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    36601 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    36661 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    36721 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    36781 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    36841 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    36901 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    36961 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    37021 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    37081 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    37141 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    37201 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    37261 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    37321 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    37381 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    37441 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    37501 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    37561 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    37621 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    37681 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    37741 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    37801 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    37861 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    37921 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    37981 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    38041 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    38101 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    38161 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    38221 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    38281 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    38341 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    38401 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    38461 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    38521 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    38581 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    38641 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    38701 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    38761 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    38821 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    38881 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    38941 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    39001 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    39061 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    39121 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    39181 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    39241 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    39301 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    39361 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    39421 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    39481 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    39541 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    39601 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    39661 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    39721 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    39781 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    39841 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    39901 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    39961 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    40021 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    40081 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    40141 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    40201 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    40261 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    40321 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    40381 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    40441 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    40501 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    40561 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    40621 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    40681 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    40741 agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc
    40801 acggatacat cagcttcaag cgacagtaca gatactacct ctaccacaag tgatagcacg
    40861 gacacatcag cttcaagtga cagtacagat actacctcta ccacaagtga tagcacggac
    40921 acgtcagctt caagcgacag tacggataca tctaccacaa caagtgatag cacggacacg
    40981 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
    41041 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
    41101 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
    41161 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
    41221 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
    41281 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
    41341 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
    41401 gataaaa
//
LOCUS       DAPCMU010000009        39015 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPCMU010000009 DAPCMU010000000
VERSION     DAPCMU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 39015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.009"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(77..517)
                     /locus_tag="RD744_000394"
     CDS             complement(77..517)
                     /locus_tag="RD744_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDU0556428.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="RD744_000395"
     CDS             complement(646..1572)
                     /gene="dnaI"
                     /locus_tag="RD744_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDU0556429.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(1572..2945)
                     /locus_tag="RD744_000396"
     CDS             complement(1572..2945)
                     /locus_tag="RD744_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDU0556430.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="RD744_000397"
     CDS             complement(2968..3459)
                     /gene="nrdR"
                     /locus_tag="RD744_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDU0556431.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(3771..4103)
                     /locus_tag="RD744_000398"
     CDS             complement(3771..4103)
                     /locus_tag="RD744_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDU0556432.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(4155..4784)
                     /locus_tag="RD744_000399"
     CDS             complement(4155..4784)
                     /locus_tag="RD744_000399"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDU0556433.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="RD744_000400"
     CDS             complement(4748..5584)
                     /gene="mutM"
                     /locus_tag="RD744_000400"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDU0556434.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(5629..8274)
                     /gene="polA"
                     /locus_tag="RD744_000401"
     CDS             complement(5629..8274)
                     /gene="polA"
                     /locus_tag="RD744_000401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDU0556435.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(8400..9083)
                     /locus_tag="RD744_000402"
     CDS             complement(8400..9083)
                     /locus_tag="RD744_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDU0556436.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(9076..9561)
                     /locus_tag="RD744_000403"
     CDS             complement(9076..9561)
                     /locus_tag="RD744_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDU0556437.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(9989..11323)
                     /locus_tag="RD744_000404"
     CDS             complement(9989..11323)
                     /locus_tag="RD744_000404"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDU0556438.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(11454..12215)
                     /locus_tag="RD744_000405"
     CDS             complement(11454..12215)
                     /locus_tag="RD744_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDU0556439.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(12231..12713)
                     /locus_tag="RD744_000406"
     CDS             complement(12231..12713)
                     /locus_tag="RD744_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDU0556440.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(12733..14913)
                     /locus_tag="RD744_000407"
     CDS             complement(12733..14913)
                     /locus_tag="RD744_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDU0556441.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(15057..15788)
                     /locus_tag="RD744_000408"
     CDS             complement(15057..15788)
                     /locus_tag="RD744_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDU0556442.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(15857..16798)
                     /locus_tag="RD744_000409"
     CDS             complement(15857..16798)
                     /locus_tag="RD744_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDU0556443.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(16978..17345)
                     /locus_tag="RD744_000410"
                     /pseudo
     CDS             complement(16978..17345)
                     /locus_tag="RD744_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(17956..19347)
                     /locus_tag="RD744_000411"
     CDS             complement(17956..19347)
                     /locus_tag="RD744_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDU0556444.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(19560..19946)
                     /locus_tag="RD744_000412"
     CDS             complement(19560..19946)
                     /locus_tag="RD744_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDU0556445.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(20002..20625)
                     /locus_tag="RD744_000413"
     CDS             complement(20002..20625)
                     /locus_tag="RD744_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDU0556446.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(20689..21357)
                     /locus_tag="RD744_000414"
     CDS             complement(20689..21357)
                     /locus_tag="RD744_000414"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDU0556447.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(21369..21809)
                     /locus_tag="RD744_000415"
     CDS             complement(21369..21809)
                     /locus_tag="RD744_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556448.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(21868..22540)
                     /locus_tag="RD744_000416"
                     /pseudo
     CDS             complement(21868..22540)
                     /locus_tag="RD744_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(22725..23123)
                     /locus_tag="RD744_000417"
     CDS             complement(22725..23123)
                     /locus_tag="RD744_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDU0556449.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(23120..23893)
                     /locus_tag="RD744_000418"
     CDS             complement(23120..23893)
                     /locus_tag="RD744_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDU0556450.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(23890..24573)
                     /locus_tag="RD744_000419"
     CDS             complement(23890..24573)
                     /locus_tag="RD744_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDU0556451.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(24740..25627)
                     /locus_tag="RD744_000420"
     CDS             complement(24740..25627)
                     /locus_tag="RD744_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0556452.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(25624..26493)
                     /locus_tag="RD744_000421"
     CDS             complement(25624..26493)
                     /locus_tag="RD744_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0556453.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(26630..27916)
                     /locus_tag="RD744_000422"
     CDS             complement(26630..27916)
                     /locus_tag="RD744_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0556454.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            28189..28767
                     /locus_tag="RD744_000423"
     CDS             28189..28767
                     /locus_tag="RD744_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0556455.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            28767..29696
                     /locus_tag="RD744_000424"
     CDS             28767..29696
                     /locus_tag="RD744_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0556456.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29693..31429
                     /locus_tag="RD744_000425"
     CDS             29693..31429
                     /locus_tag="RD744_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDU0556457.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(31492..32328)
                     /gene="pheA"
                     /locus_tag="RD744_000426"
     CDS             complement(31492..32328)
                     /gene="pheA"
                     /locus_tag="RD744_000426"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDU0556458.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(32325..32834)
                     /locus_tag="RD744_000427"
     CDS             complement(32325..32834)
                     /locus_tag="RD744_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDU0556459.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(32827..34122)
                     /gene="aroA"
                     /locus_tag="RD744_000428"
     CDS             complement(32827..34122)
                     /gene="aroA"
                     /locus_tag="RD744_000428"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDU0556460.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(34135..35226)
                     /locus_tag="RD744_000429"
     CDS             complement(34135..35226)
                     /locus_tag="RD744_000429"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDU0556461.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(35243..36409)
                     /gene="aroC"
                     /locus_tag="RD744_000430"
     CDS             complement(35243..36409)
                     /gene="aroC"
                     /locus_tag="RD744_000430"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDU0556462.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(36411..37475)
                     /gene="aroB"
                     /locus_tag="RD744_000431"
     CDS             complement(36411..37475)
                     /gene="aroB"
                     /locus_tag="RD744_000431"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDU0556463.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(37508..38530)
                     /gene="aroF"
                     /locus_tag="RD744_000432"
     CDS             complement(37508..38530)
                     /gene="aroF"
                     /locus_tag="RD744_000432"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDU0556464.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT        10861 a         8551 c         6812 g        12791 t
ORIGIN      
        1 tcccatatct ccttaaaaaa tatacctttt cgttctataa aacaacattt tcttattttt
       61 gaaaaaatgt tattggttat ttgttcaatg aaacatcaat aatcgaatct ccaggaactt
      121 gcggtgggat agacatcgtc atgatcggca tctcaactaa tgtaaattgg actttgtcac
      181 cgattttcaa ttcatttatg tccacgctta ttttttcagc agtagcattt aagatcactc
      241 cgtcattgct gaatgacgtt cccatattcg ctggatcttc tttggccatg acatcgacga
      301 gtttcaactg aagcgtttct tcttctgctt ctacttgagt aatactttca atcgttccga
      361 caaatatgct ttgtctctcc gttttcgtac tgcttgtttt tactgtttga cccgcatcaa
      421 tcgaatcaac agaacctgtg ttgctatctg cgctaccgca gcctgctaaa agaaaaaata
      481 gaccaactac agatatacta gaaaaaatag ccctcataaa aaatcctccg tttaagtttc
      541 tgcattactt gttgcggtga ttgtctccgg tcatttctgt ccatataatg attcgctgac
      601 ctgcttttac tactaggtca aatgtcagtt ggacttgtca actgttcaac cattgcgtcg
      661 gttgcgacca gtcatttcga tttcttttgt caaataacgt atgcgttcca ttatccgttt
      721 cgcttttagc ggctcttcat ctccacgttg agtgacggac aaatgctgtt ccaattcatc
      781 cattgtaaaa ttcgatgaga agaaggttgg taactgttcc tgcatccgat attgaaggat
      841 aacaccgaat acttcgtctc gtatccaact actcatcgca tctgcaccaa tgtcatcgat
      901 catcaaaata ggtgaacgtt tcactgcatc taacttttct gccacttgat cttttccgat
      961 tgcctgtttc atctcaacgg caaatgtcgg aaaatggact agcgtcgtgg tgaatccagc
     1021 aatcgctaat tctctcgctg ttgctcctaa taaatatgtt tttccaatac caaaacttcc
     1081 tactagatac agtcctttat gatacccttt tggatcagca gtatatttct ctacaaaatc
     1141 tagggcttct aagctcgcat atcctcgtcc atctgtacgt tcaaagtttt caaaagaagc
     1201 cgattgaata tcttttggca tatccattgc ttttactcgg cttctgattt cttcttgtct
     1261 ttgccgtgct aacaatgctt ccgttggtac ataagtgaca tccacataat gaaaattcaa
     1321 cgtcagcttc ggctcatagc cgggagcgat catcgttgga tcgttttttt gatatttacg
     1381 cttttcttgc acgaattcat acaatttagc gtaacttttt tcaatatccg cttgtgtcaa
     1441 ttcttcttgg tgtgcgctta gaaaagcttg aacatccgca tcatttaaga cttcagcgat
     1501 catttcagca tacttttctt gataattgcg tttgctgatg atcctcttca attctttgcc
     1561 tacgtcttcc actagatatc accctccttt tttgccaggt attctttaaa acggcggtca
     1621 atctctgctt ttttctcgct gctgatctgt ttttcatctt ttggctggtt gacccaatca
     1681 ggaagctttt cccgtcgaac atttcgcttg ccagtttgat aatttgtttg cttcttttgt
     1741 tttgattgtt ttgcttgatt acttatttgc cgaatgtgct ggatcgcttc ttctgctgtg
     1801 gcgattttct tttgtgccca ttcatttgcc actgtattta cataactggc atttaagcta
     1861 gcattattct tgatcactag tacataattc agcaaaacat tgatcacgct actggaaaga
     1921 cctgattgag aaacaagatc ttgcagtaac cagttttctt gtttagaaac atatccgcct
     1981 ttttctgact tgattgcttc cagatatttc atcggtggaa tgctctgtgc ttcttttatc
     2041 agctgaagcg ctgcgtttgt caaatgttcg tttgacgagg gttcattact gttttcagaa
     2101 accgcaactg tttcttttgt ttgcttcacc ggacgtctct gcattgccaa aatggctttt
     2161 tgcagttctt tttcagatac tttttgttca ctaaagtcat aagattgact gatcagttcg
     2221 ccaagtgtca attcatcgaa accgtaaagc tgatgataca tgtcgacttg tttgatgatt
     2281 ggatcatcaa gacttgcaat atgttttttt gccagccaat cattcaaaaa ctgccaatcg
     2341 aatgtcgaat ctgcttcaag taatgctact gattcagatt cttcaaaaga tttattcagc
     2401 gattcgatgt tttcttgatt agcagcgtac actgcttcat tgaagcgata aacatctaaa
     2461 aatttttttg tgattttttg atatccctct gctgtatgcc ttttaggttc aaagaattgg
     2521 gtaagcttct ggaatcgtcg ttcaccaact ttttccagaa gaagaaaagc cagaacagag
     2581 tctttgaaaa atgctttagg aatgagtggt tctaacagct cataaataaa acgaggtccc
     2641 agatcactat cttctttaaa ataaacatcc agtaagccga ttccttctag cttttttctt
     2701 gcatcataaa attgcgggat accgcagcct agactactaa gcagatctgc atgcatccct
     2761 tcaggacctt tgccagattc ttgagagatt tctgataaca gggtgaaata gatactcaag
     2821 gcttctccgc cgattagtgg ctgataaaga tacagcaagg cgttcttgct gtcttctctc
     2881 agcggagccg cttttcttcc ttggtaaata ttttttggtt gaagctcttt ccaagcattt
     2941 tccaaggaaa tgcctccttt ttgtttctta attgttttct ttattattct ctttattatt
     3001 ttcttttgct ttatccacga tatcttgcaa ctcttttaag aaaacagaca tatctttgaa
     3061 ctggcgataa acacttgcaa aacgaatata agcgatttcg tctaaatcaa ctaaatcttc
     3121 catcacatat tcaccaatta gattcgtcga tacttcgttt tctccgagac tgcgtacgcg
     3181 gttttcgaca tggttgacga tttgttccat ttgttccata gctacaggtc ttttttctgc
     3241 tgaacggatg agtcctctta aaattttatc gcgattgaac tcttcgcgtt ccccgttttt
     3301 cttgattact aataacggag cagcttcaat tcgttcaaat gtagtgaatc ggaacgaaca
     3361 attctcacat tcgcgacggc gacggatcgc acgtccatca tctgcttgac ggctgtcgat
     3421 tactcgtgaa ttattatgtt gacatctggg acatctcatt ttgtctcacc tcttgcatcg
     3481 cattttatat aaaggatgtg tctttcttta caactagttg tattataaca cgaaacaaat
     3541 cacaaacgcg actgtcctac ccaaaaaaca cggatataag aacaattttt tctactttca
     3601 tgcgtaattg tgtcccctgt ccttactaaa tttcaatttt ccaatactga tcaccttgat
     3661 tactgagaaa taacttacac aaaagctgat ttagtcatct agcacaaaaa acttgaagca
     3721 gatgaacacc ttctcatctg cttcaagtca catctcgatt ttttactatt ttataacttc
     3781 tcttcgattc ctgtatagtg tttcattcgc tgttcctcga ttttttgctc atcggaaatg
     3841 tggtcacttt ctgggagcgg tgttttacta cgatcaggtg tcggattttt tcgttcttga
     3901 gtcaaatctt gccgcttcgc ttcttcttct gcctgctggt cttctatttc atcgattctt
     3961 gcttggtact gcttttcttt ttcttctgct gtttctgatt gcgtacgacc ttcatcgatt
     4021 agattttctt tattgagcat ctcgccgatt ttttcttttg cgttacctac gactttatcc
     4081 ttttgatttt ttaatgatcc catcgctatc cctcactttc tatatactca ggatagcaaa
     4141 attgacacag atactcaaag aaagcgatta gcaaaaagcc aattttcgat ttgtcccaca
     4201 agtttttctt ttgttccatt gttgtcgaac aaaatatccg cacgctgctt tttttcttcg
     4261 attggaagtt gactactaat tctctgctgc gcttgttcta gcgtcagttg attacgtttc
     4321 atcaaacgct gaagctgagt ttctggcgta gtataaacga cagctactgc atccatatag
     4381 tggtcataat gaccttcgta gagcaatggg atatctacaa ttactaaagg atgattggaa
     4441 cttagagagg cgatttgatc ttttatcgct tttcggataa acggatctaa tagctgatca
     4501 agtttttgcc gtttttcctt tgatggaaaa acaatcatcc caagtttccc tcgatctagt
     4561 tgcccatcag gctgcaatac ctcttggcca aacactgata caattttcct cagagcaagc
     4621 gttccaggct caacagtttc tctggcaatc acatcgccat cgatcaaagg aaatccatga
     4681 gactgaaaga ctttggctgc cgtactttta ccggtagcta ttccacccgt caaacctaaa
     4741 acaaatccta ctttgcgatt tttagccttt gacaattcgg acaatagtgc gtcccccttt
     4801 gtgccacttt tgttttcacg atcggcgttc cacaacgtat acatggtttg ccagtttgct
     4861 gatagacatg cagtgctact tgaaatccgc ctgattctcc taatgcattc aaataactgc
     4921 gaatcgtggt acctccggct tctattgcac gatccaaaac atcaatgatc gatagacgta
     4981 attgttctac ctctttgggg cgcagtgtgt tcgccggttg ttctggatgg atcttcgctt
     5041 cccataatgc ttcatctaca taaatattac ctagaccagt cactagacgt tggtctagca
     5101 gcaatggctt gattgctttg tgggactttt tcagtccatt tgcgaaatct gctaataaga
     5161 agctatctgg caaaggctca ggtcccaatt tcatgattcc tcggtaagtt gcacttgctc
     5221 ctttttcgac gattgtcata cgaccaaatt ttcgtacatc gttgtagcga agttggcttc
     5281 catcctcaaa aaagaaaagt acatgcgtat gtttatcgat tggtgtattt tctttcgtaa
     5341 attgatattt tccttccatt cgcaagtgtg agatcagttc acaatgatcg agatgaaaaa
     5401 ttaaaaactt ccctctacgt ccaatggatt ggatggtttc gccaacaaga gaagcctcaa
     5461 agatgggtgt ctctggttgc tcgatgatct tgggccacaa gacctgaact ttttgaatcg
     5521 tttttcctac tactaaccgt tccaatcctt tacgaacggt ttctacttct ggtaattctg
     5581 gcattttttc ttttcacctt cattatattg ctctgtctta aacaatgttt attttgcttc
     5641 gtaccacgtt tttccccaac tgctgtcagt gatcagtggt acatgaagag aaacagcttg
     5701 ttccataact tccttcacca atttatctaa tgattcaagt tctttttccg gtacctcgaa
     5761 gacaagttca tcatgaactt gtaacagcat cgtagcttga agacctgttt cttttaatcg
     5821 tttatccaat tcgatcatcg cgattttcaa aatatcagca gcacttcctt ggattggtgt
     5881 attgatagct gttcgttccg caaaagaacg gatattgtaa tttcttgaat tgatgtctgg
     5941 cagatatcga cggcggtgat acagcgtttc gacgtagcct tggtcttttg cttcgcgtac
     6001 gattctttcc atatattctt tgacacctgg gtatttttca aagtacgtat cgatgtattg
     6061 ctgtgctgct tttcggctga ttcctagatt ttgtgaaagt ccgtaatcag agatgccata
     6121 cacgatacca aaattcactg cttttgcctg cctacgcata tttggtgtga catcctctgc
     6181 cttttcgatt ccaaatacac gcatcgctgt gctggcatgg atatcttgac cttctaggaa
     6241 ggcagctttc aaatgttcat catttgagat atgcgcaagt acgcgtaact cgatttgtga
     6301 atagtcagaa gaataaatca gccagtcttt ttctctagga acaaatgcct gacgtatctt
     6361 tctcccttca tctaatcgaa tcgggatgtt ctgcaaatta ggatcaacag agcttaatcg
     6421 tcctgtttgg gtcaatgtct ggacataacg ggtatgcact tttccatctg attggatcac
     6481 tttcagcagt ccctcgacat aggtagattg aatttttgca atttgacgat aggtgaggat
     6541 atcctctacg attggtgctt gttcacgaag ctgttccagt acatctacgg ctgtggaata
     6601 tccagttttt gtttttttga taacgggtaa tcccattttt tcaaataaga tcactcctaa
     6661 ttgctttggt gaattcaaat tgaattcttc gccggcttct tcgtagattt tttgttcgat
     6721 ctctcttaag cgagcagaga actcgccctt catttcttgt aagcgagtcg catctacttt
     6781 gatcccgctt atttccatct ctgccaaaat gatagacaat ggcagttcca ttttccggaa
     6841 aagatcttct tggtttttat ctgccagttc ttggctcaat tttggtgtca acgcattgat
     6901 tgcagctact tttcgcgcta aatgagcaaa aaagagttcc tcttcttctg gtaaaccttt
     6961 tttcgcccct ttgccgtata cagcttcatc tgattggata tcgttgtatc cgtaatgagc
     7021 agctattcct tcgatatctg tgcttttttc attcgtatcg agaaggtatg cacctagcaa
     7081 gacatcaaaa tcgattcctt tcgtttttcc agtatagcgg tttaaagcaa cataagttcg
     7141 tttcgcatca tatacttttt tcagccgagt cggatcagtg atccaagaat ggaacgctcg
     7201 gttttcgaat acagatagat cgttcgtgac ataaattttc ttttctgtcc cccacgctac
     7261 tccgacgatt tcctctgtat gataattgtc gcccatcatt tccacgtaca acgccatatc
     7321 cgtagtaaac atttcctctt tgaattcatg aacgacttca aacaaaatat catccatttt
     7381 tacttcttct tctacgatat tcaatttaga aaggaactga ttgaaatcca tttctttata
     7441 aaacggaacg agtttatcga gatttttccc ttcatacttc aatgagtcga tagagacatc
     7501 cacaggcgct gaagtattga tggtcgccaa ttttttcgat aagaaagcct gctctttgtc
     7561 atttatcaga ttttctttca ttttgctttg cttcatttca tcgatatgtt cataaattcc
     7621 ttcgactgag ccatattgtt tcaatagctt gatagctgtt ttttcaccga ttttagtcac
     7681 accggggata ttgtcagagg catctccggc tagtcccttc atgtcaataa tctgttttgg
     7741 cgtcaaccca tcatattttt ccgctacatg ttctggtgta tagctttcga tatcgctgac
     7801 accttttaca gtgatatcca cttttacttc tttactagct aattgagtca aatcccgatc
     7861 acctgaaagc acgacgacat caaattgatc tttgtctact ttttctgcca aagtaccgat
     7921 aatatcatct gcttcataat tgggcaattc gtaatactgt acccctagtc ctgtcaacaa
     7981 atcacgtata tatggcattt gttctttgaa ttcgccaggt gttttggatc ttccaccttt
     8041 gtactccgca tacatttctg tccgaaaagt cgtttttcct gcatcgaacg caaccagaac
     8101 atgagtcggt tgctcttttt gcataacatt ttcaaacatc gtattgaatg catagattgc
     8161 atttgtatga agaccgtttc tatttttgaa acgttcaaga gaattgtgaa gcgcaaagaa
     8221 tgcgcgaaaa gccacactgt tcccatcaac gagtaataat ttattttttg tcatcttctt
     8281 aactccttac tgtaaagttc ttattcattc taacaaagaa tcctgcggaa tgcgaaaaga
     8341 aaaagcggga ccataccaaa taattcggta tagccccaac tgatgaattt ttttcttttt
     8401 tagttgttcc gtccaaagat tctcagaaac gccaagaaca agttgataaa gtccaaatac
     8461 agcttcaatg ccatgaatac agcaattcca ctatgtgctg ttccgtttgt ctggacatag
     8521 atattacgga tgctttggtt atcataagct gtaatccctg caaagatgac gactaacagt
     8581 agggagatga acagctcaac tacgctactt ctcaaaatga agatgttcaa gactagtgca
     8641 atgatcactc cgattaaagc tgcctgtcct gctcttccaa ccccgctaag atctcttttt
     8701 gttgttactc cgacaacaga catccccatg aacatcaaag tagctgtcaa gaaagcttgc
     8761 gttactgttc caatatcgta gtatgcaaga gtaaccgcta atgttatgcc attcaaaaga
     8821 gagtaggcaa taaatccacc gattgctaag ctcggattct tttgtgcttt tacgcctaga
     8881 accattacta aaatcagttc aacgatccac agacccacaa agcctagtgg aaaattattg
     8941 ataaaagttg ctaattgcaa aggaaatacc caaagagcca agtaagaagt gactgcacta
     9001 agggcaagtc ccatacctaa aaatccataa acttttgcat aaaaacgatt gatacctgac
     9061 attgatgtac ttggattatt cattcaattc accttcactt tcgattgcta tttccaattc
     9121 aggaacttgt ggcggttgga ccataacgac agcatccaac acacgatctt cgttcgtatt
     9181 ctccgcttct acagaaatcg tcaccacatc cttcatctta tctactttta tgacctcaaa
     9241 ctgaaaagtc agtgtttcat aatggaaaac cggttcaata aaattgacag aaaaattgac
     9301 aacatgtgat cctggtccag gaagatgctt tgaaattgca cttgtgatga tccccatcag
     9361 catcactgat ggaacgattg gctgttgata ttctgttttt tgtgcaaagt catgttggat
     9421 atacaaagga tttgcatcat tcgtcaatcc caaatacagc aaaagctggt tatcttcaat
     9481 agattctgtt aaggataacg agtcgccttc ctcgatttca tttattgttt ttcctagttt
     9541 tctcggctta cctatgttca agatagaaac cctccagttt atttttaatc atacattatt
     9601 gtaccaaaag tagcgtaaaa ggcaagttct gctcccttga tttaacagat tcctatagtt
     9661 tacagaggtc gtgaaaaagc tgtcctgcat caaataataa gaacagccaa gaaaaaacag
     9721 cttctcatat ttttcatttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt
     9781 ttatctgagg tcgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagaaaaaca
     9841 gcttctcatg tttttgattt gattgggctt attaccgcag atgcaagctt ttgaacaccg
     9901 tttatcataa gttgcaaaaa agggggcttg gcaagatttt tgccactccc cctctttcta
     9961 tttttataag gacactgcag taattgtatt acagtacatt tttcgtcgtg ctgcttaaaa
    10021 gtttttcata cgcttgttca aatttttgaa catctccagc acccatgaag attactactg
    10081 catcatgata atctaacaat ggagatacat tattttcttt gatgacttct ccaccttttt
    10141 tgattttcgc tccaaggtct tcaatcttaa cgtttccttg ttcttcccga gccgaaccaa
    10201 aaatatcgca taagtaaact ttatcagcta aatcaagtgc ttctgcaaat tcatccatca
    10261 atgcaatcgt acgcgtaaac gtatggggtt ggaaaactgc aattatttct ttatctggat
    10321 atttttggcg tgcgccatca atcgttgctt tgatctctgc agggtgatgc gcataatcat
    10381 cgactacggt catgtctgct acgatctttt cactgaatcg acgttttact cctgggaaag
    10441 tcagcatttc ttctgcaact tctttcagat ccagtttttc gaaataagct actgcaataa
    10501 cacctaaagc atttaagata ttgtgtttac cgaatgctgg tacagtgaag tgcccaacaa
    10561 attcatcccc atgatagaca tcaaaagcag aaccagaagt tgtgcgttcg atgtttcgtg
    10621 cttggatatc atcgttttct gtgacaccat aataataaat cggcacattt gcttttaatt
    10681 tgcgtaaata agcatcgtca ccatacgcaa aaatagcttt tttcacttgt ccagccattg
    10741 tttggaaagc tgtaaataca tcgtcgatac ttgtataata atccggatga tcaaaatcga
    10801 tattggtcat gattgcatag tctggtgaat aggcaaggaa atgacggcga tattcacagg
    10861 cctcaaatga aaagaattcc gcttgcggat caccatgtcc tgttccatct ccaattagat
    10921 aacttgttgg acggacacca ctcaatacat gagagagcag tccagtcgta cttgtttttc
    10981 cgtgagagcc tgttacagcg atactcgtat aattttggat aaaatgacca ataaagtcgt
    11041 gatagcgaat cacttccaaa cctaattctt ttgctcgctg gatttcttca tgtgaatcag
    11101 gaaatgcatt tcctgcaatg atcgtcatcc ctggttttac attatctgcg ttaaatggca
    11161 gaatcgtgat atttgctttt tctaaatctc tttgtgtaaa gaaatatttt tcaatgtctg
    11221 atccttggac atttagacct tgttcatgta aaacaagagc caaggagctc attcctgaac
    11281 ctttgatacc aacaaaatga tacaatttat tttgattttc catttgaatc ctcttctctt
    11341 atctagcgtc cattctttta gctattacat tcaacacgct cctcattatc ttataaacga
    11401 ttgaaaattt caaccaaatt tatttggaaa ggaggattcg cttgctaaaa tggtcaagta
    11461 cgggaaaaat ccgtttttat tgactcttta attctggtgt aagaagacca aacttcctct
    11521 gatatatcaa tgtgaggaat aaaatctttt cctttcggac catagccgtt ctcatcgtca
    11581 tggagacgtt ccatttctcc taatactagc gcatggaaat attccttagg ccattgatga
    11641 ttcagttctt ttgctgtaaa gatgatctct tcagaaaagt gaaggatttc cggcacaaat
    11701 ggcacagcat tcgcgaaaat agtttgttca ttttgccata ctttctcaaa agttgctcga
    11761 gtcctgcgtt gcagtgtcgg atcattcatc aataagattt tttctggtac agctcctaag
    11821 gaatgaagaa tctccagtga aaaagtggcg ttctctccag aatttgtaga cttcgattct
    11881 atcagaaaag ccttatcggg caaatcatag acttctttta gatactgacg acagatttca
    11941 ctttcactca tgccatcttc aaaatgaaat ccttgctttt caaaattctc ataaaggatt
    12001 ctagtcgcat gccccacccc tccgaccaaa aacaattgat ctacttggcc atttaaatac
    12061 atttgtgctg ctttatcagc taagatagga agacagtttc cagctaaaat aagtgtaggt
    12121 gccgtcagtt cctcaaaatt tccctcttca ggtgcggcta aaaaatttaa tagctgattc
    12181 cagtctttta gattcctctg ctcacttttt tccaaaggcc ttctatcctt tcatctgatt
    12241 ttttgtgctt cattaaagtc ttctcttttt gtctcaatcc atttgatttc tccatgacat
    12301 cttccacaac gatatttgcg tacatcgatt cttctcttgc ggcagatcgt ggcagaacaa
    12361 tcttggcatc gataatactc catctttatc tgtttcttcg tcaaggctgg tgcatagcgc
    12421 aaccctcctg tttttttcaa aagatacttg aaatccatat ctttatgctg ataccctctt
    12481 ccagctagat gaagatgata atggcaaagt tcatgtttga tgattcctgc aatcgtctct
    12541 tcgtctgact cttcaaatag cttcgcatta aaatctaaat gatgatcgtt taaatgatag
    12601 cgcccgccag ttgtacgtaa gcgtggattg aacattgctt ggtgcttgaa aggaagctgg
    12661 aagcttttta atgatatctc ttctactaac tgctgcagtt cttcttgtgt caatttttgt
    12721 tactcctcat tgttactctt cataagggga aagcatcgtc aaactgattc tgcctttttt
    12781 cgtatcgact tgttctatcc aaactgttac gatatctcct actgacacga catctgtagg
    12841 atgcttgacg aattttttgc tgagtttcga tatatggaca agtccgtctt gcttcacacc
    12901 gatatcaaca aatgcgccga agtcaatgac attacggacc gttccagtca attccatccc
    12961 tggtttcaag tcttccatac tcaatacatc tgtacgtaat aagggcgctg gcatctcatc
    13021 acgcatatct cgtccaggtt gtgtcagccc agctaaaata tcggctaacg tttcttctcc
    13081 gactgatagg ctttcagcca gtttctctac ggataatcgt gtcaattttt ctactgcttc
    13141 ttctgtacca agctcttttt ctgaaagttg gactgaagtt aatatttctt ttgcaataga
    13201 ataactttcg ggatggatac ctgtgttgtc caaaatgttt ttcccaccag ggactcgcaa
    13261 gaatccgatt gcttgttcat atgcttttgg tcctaagcgt ggcacttttt tcaattgagt
    13321 tcgtgcggtg aattctccgt tttcttcacg ataaatgaca atgttttggg cagttgtttt
    13381 attcaatcct gatatatgct gcaagagctg cgggcttgct gtattcacat ccacacctac
    13441 ttggttaacc gctgtttcca caacaaaatc caattgttca gccaaacgtt tttgagaaac
    13501 gtcatgttgg tactggccta ctccgacagc tttaggatcg attttgacta attcagctaa
    13561 aggatcttgc aggcgacggg caatagaaac agcacttctt tcttcaactt gtagatgcgg
    13621 aaattctttt cgtgcaattt cgctagcgga gtaaacagaa gcgcctgctt cgttgacgat
    13681 agcgtaatag gttttatggt cggcggattt caactgctca gccacaaaaa gttcagattc
    13741 ccgactggct gtaccatttc cgatagctac catatctact tgatatttat taattaattg
    13801 gataaaagct ggtccagcag cttctctttt tgcttgcgct gcaggtttat gaggataaat
    13861 aacttcgatt gctaaaactt tccctgtcgc atctacgact gctagtttac atccagtacg
    13921 ataagccgga tcaaatccca ggacaacttt accttttagt ggcggttgca acaaaagatt
    13981 acggaggttt tcgccgaaaa tagcgattgc ttgctcatct gccttttcag tcaattcatt
    14041 gcgcagttct cgctcgattg ctggctgaat aaagcgttta taactatcct gataggcttc
    14101 tctgacgaat gaagaagaag gagaggcagg atttttgact atttggcgat ccaagtaagc
    14161 taaaattgct gcttcatcta cttgaaagaa tactttcaga atatcttctt tttctccacg
    14221 attagtagct aatattcgat gagaaaccat tttatggacg ggttctgcaa agtcataata
    14281 catttcatat acgccttttt catctgcttg ctcatccttg acattgctga catacatacc
    14341 tttgttatac gtataagacc gaatccaagt ccgaaatttt gcattatcgc tgacttgttc
    14401 agcaataatc tcatgagcac cgtgcaacgc ttcttcggca gaagagactt ctttttcttt
    14461 gtcaatatat ttttctgctt cagattgcac ctctccatct gtaagctgca tcagccactt
    14521 cgcgagtggt tcaagtcctt tttctttagc aatcgttgct tttgtccgtc gtttttgttt
    14581 gtatggacga tacaaatctt ccacttgttg cattttcacg gcttgggtga tttctgcttc
    14641 taactcggga gtcagtttcc cttgttcatc gattagccga atcacttcag ttttacgttt
    14701 ttctaagttt tcaagatagg cataccgctc ttcgatttca cgaatctgga cttcatcaag
    14761 acttccagtc atttcttttc ggtaacgcgc gataaaagga acagtgtttc cttcatttaa
    14821 caaattaagt acagtagtca actgcttagg acgatagtct gaaagttctt tttgtacaag
    14881 ctgaatgatc gtctggttca aattttccgt cattaaaatg cctcttttct gctaaaaatt
    14941 gcttctttat tctatcatac tttttatcct gggttaaact actgaaagaa acaatattct
    15001 ctataaaaat aaaaagagcc gcaacaaagt cacagctcct tcattctttc cattagtcag
    15061 attccatgcc aaggtccttc gttgttctga tattcaatat cttgaagaat accttccttg
    15121 tcaaaaacaa ccccatgcaa tgaccctccg aatacagccc caccgtccat tccgatttta
    15181 tgatcctgaa tccagaggtc tgttgtctgc atatctccat ggagcattgg tgtaatcgta
    15241 tgtccaaata caattgtctt acctgtattg tttttccctt catgaaatgg ttcccttatc
    15301 caaataaagt ctcttagact tgtcttacgc cagtctttct ttgtcaaatc cacaccggca
    15361 tggacaaaaa gataattttc ccattcataa tacaaggggc gctggactaa aaatttaatc
    15421 agttctgaat atctcgattg gatcatcatc gagatttcag ttggggagta ttcctccgat
    15481 gcccctgggt gaatcaggct ttcaaacgtt tcttttccac catttctcat ataaggcgca
    15541 taccagtcct ccggtgattg cagaaattgc aaaaaatatt cttcatggtt tccccggagg
    15601 taaatggctc ctgtttcttc gactaattgc tttcctaata aaaaacattc ttttgtacga
    15661 ggacctcgat cattgagatc ccctaataaa acaagctgat ggatctttgg atcataatag
    15721 tgaataagtt tttcaaaaag ctcatgtttg ccatggacat ccccaatagc ataaatcaat
    15781 gatttcatgt ggatcaccat cctcaacgct agtatagcgc atattggaaa tggtcgacaa
    15841 tcaaaactat tgttttctat ttaattaaag ctgtaaaccg cagttctgct tcttctggag
    15901 agattccatg gtcttgaaca tagcgattgc gcggatgttt cgcacactct atgctacatg
    15961 ctcctaaatg tttttcttca ttttcaatgg aagcaagaat ctgacgattg cactcaggat
    16021 tgctacaatt gatatatcgt tcacatggtg ttccgtcaaa ccagtcgcga ccgacgattt
    16081 gtttatctac ttggttgata tcgacagcga tccgttcatc aaacacatac atcttgccgt
    16141 cccataattc tccttggact tcaggatcct taccataagt agcaataccg ccatgcagct
    16201 gtccgacatc tttgaatcct tcacggacaa gccaaccaga aaacttctcg caacgaatgc
    16261 caccagtaca gtaggtcact acccgttttt ccataaattc ttctttgtgc tcgcggatcc
    16321 agtctggaag ctctcgaaaa ctgcgtatat ctgggcgtac agcccctcga aaatgtccca
    16381 agtcatattc ataatcattc cgtgcatcaa tcaccactgt ctgttcatct aaaatagctt
    16441 ctttaaattc ttgcggtgaa agataggcac ctgtcacttg gtttggatca atgtcctctt
    16501 ctagctttaa acttacaagc tctggacggt atcttacaaa catcttctta aaggcatgtc
    16561 cttcgcttgg atcgatcttg aacatcgtat cagcaaatcg aggatcagct ttcattgctt
    16621 tcatataggc agtcgtgttt tcaacggttc cagacacagt accatttatt ccttcttcag
    16681 ctactagaat acgaccttta agatccaaag atttgcaaaa ggctaaatga tttttggcaa
    16741 attcctctgg ttcttcgatt tttgtataat tgtaataaag cagtacttga taatccatgt
    16801 atttcttcct tccttctttt cgttcctaaa cagtataaag aaggcgttcg catttgacga
    16861 tttatttgct tgtgtataat tacgcatgga aagttttttg tttacataat attattattt
    16921 gaattaaaaa gacttcgctt tcctgtttcg cacagaaaaa caaagtcttt atttctatta
    16981 tttacctgta accgcaaagt agttgccatc gggatcagca aagttaaaga catattcttc
    17041 tccaagctgt accatatcac ctaatgtgac tgattgcgtc tgcatttttt tatatagtcc
    17101 aaagatatct ttactgtaaa acatcaaaga tggggcacta gtagctgtgc caggtgaatg
    17161 ggactcaaca aatgctcgat catataacag aaatgaggtt tgactgtcct caactcctac
    17221 ttcaattata agcgtcccat ccatttcttg acgatctttt tccgaaagcc aactgctctc
    17281 caaaacgttg catttttttc tacatcgtcc acataaagca tgatttttat ttcattcgta
    17341 aacatctgtt tcctcctaaa cgatctgttc taacattctc aactttgtca gtttaccata
    17401 ggaaaatggg aaaatctacg aaagctctca acagaatcgt cctacttaaa tacgcttttc
    17461 tttaataata aataagttgg ttgtttttgt tatttacgct ttcacttttc ggaataaata
    17521 atgtgtattt atatggaata aataaaaaaa gaggtcgtga ctaaaaacaa gtcacaacct
    17581 ctggtaaacg gtgttctcct gacaactcct cggtcacaag acacaatatt caacaaacat
    17641 gagaagctat tttttgaaat tgctctttgt gactcagagt gaaacggcag gctcacgacc
    17701 tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagctcaac caaacgtaaa
    17761 atatgaggaa ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    17821 aacctcttat tcacggtgtt tcagaagtag cttctcggaa ataagccgaa atttcacaaa
    17881 aatttgagaa actattttta ggaaacttct tcttatttct taaagctgac cacttctatc
    17941 acaacctctt tttgactatg cattcacatc gatcacttta gaatcatcat tgatgacatc
    18001 gcgatctaaa tctttgatgc gtgcacttgt cacgatacca gctaccatgc taccgctgac
    18061 gtttactgct gtacgtgcca tatcgattaa tggttcaaca gagataacta acccaacgat
    18121 tgctactggc aagttcattg cccctaaaac gatcagtgaa gcaaatgttg cgcccccacc
    18181 gactccagct acaccaaaag aactgattgt tacaattgcc acaagcatca aaataaattc
    18241 caaactaaag acattcactc cagctgctgg tgcgacgatc gttgccaaca tagctggata
    18301 aacgccagca cagccgtttt gtccaataga caaaccaaaa cttgctgaga aattagctgt
    18361 tgcatcatct actcctaaag cttttgtctg agtctcgata ttcaatggca aagctccagc
    18421 acttgaacgt gaagtaaacg caaagctcaa taccggaaag gcttttttga aatagttgat
    18481 tggattgact tttgcaccaa tcaaaatcag tgagtggaca atcaaaacag caagcaatgc
    18541 tacgtaagaa gcaacgacaa atttacctaa attgacgata gctgcaaaat cacttgttgc
    18601 caaaacgttt gtcatcaaag caaatactcc ataaggtgtc aaacgcaaaa caagcgtcac
    18661 gatccgcata atgattttat acagactgtc gactaaatct gaaaagaatg ctgcttcttt
    18721 tggcgctttt cttttcacgc ctagataagc gattccgaca aatgccgcga aaataacgac
    18781 tccaatcgta cttgttgcac gactacctgc taaatcagca aaaacgtttt tagggataaa
    18841 ggataaaatc tgctgtggaa tcgataagtt ttcaactgtt tcctgacgtg ttgccagttc
    18901 agagattcgt gctgtttctg cagctccttg tgtgaattta gctccttgga gtccgaacag
    18961 cattgttgaa ccaattccga tcaatgctgc aatagctgtt gttccaagta aagaagtcaa
    19021 tacagtcgca ctaattttcc ctagcttttc agaaactttc atttttgtga atgcaccaat
    19081 gatcgaaata aacactaaag gcataactag catttgcaag aatgatacat atccttgacc
    19141 tacaatcccg atccattcca ttgattgagt aacgactttg tcgtctacac caaagatcaa
    19201 ttgaagcaga ccgccaaaga cgataccaac tcctaatgct gtaaagacgc gtgttgaaaa
    19261 ttttgtatgg cgcttttgca tacgatagaa cacatataat aatgccgcaa aaactagaac
    19321 cactaaaact gtgatgagtg ttgtcatttt tctcctcctc atattattaa tgacaagtcc
    19381 atcgaaggaa gtaaaggttc ccgagaatga ttcttcaacc atcctcctga aaaaaatttt
    19441 ttcagtgact tttatatttt agacgatttt tatcgaaatg acattctttt tgcttacaat
    19501 tagtaataaa aaaacatacc tgtgatagct ttaatctatc gcaggtatgt ttttactgtc
    19561 tattcttgtt tttctgattg aaaattcaat tttaccccag gctgatctaa ggcgatctct
    19621 gtcacctcat aagacattcg gcgtacgata tcgaataacg gtgctactag ctcgtccact
    19681 tcttcttgtg tgacatcatt ttgaccttca attttccgat tgaggatatg attgatctga
    19741 ctcacacttc cagataaaac atatgtatca aacactaaac ggaactctaa gcgtgctcct
    19801 acgatgctat gttctttcgg atagttttcg atcgtttctt ttaaaggcga gaagcctact
    19861 ttcaaatctg tttctacttc ttggttcggc aatctcatat cataatgaaa tgcttctaca
    19921 acttcttttt gtcttctgat ttccatataa ttttatcact cctttatgaa aattataaca
    19981 tatctccttt gaaaggggat actacttttt aatgatgctc tgcaaggtaa ggatttcatt
    20041 cagatttcct accacataat ctgccgggat ctcacccaga aaatgatcga tgtcgtaaag
    20101 acaagtagcg actcctgcat ttttccctgc ttcgatatct aaacgacgat cgccgatcat
    20161 catcgtgtct gttctttcta aatgaaaagt atcgatcaaa taatttaaag atgctggatc
    20221 aggtttacga ggaaagtcct gatcgatccc tactacttcc tcaatcagat gtgccaatcg
    20281 atgttctttt aacaggcccc atgttgattc tgtcaaacgg tgggtcagga tgaaatgacg
    20341 accgccgttg tcttttagca tttccagtac ttgtttcgtt tctggaaatg gacgggaaac
    20401 ttccgtactt tctttttcat atcggtgaaa taattcatta aactctctgg cattcaagtt
    20461 tgattcattg atcaaataac tagtagaata ctttttcatt ttgaagtaga tttctttttt
    20521 gtccatactg ataccaaaat cttttagtgc ttgccatgca ccatctacca ttgcgggata
    20581 cgtatcaaac aatgtaccat caaagtccca tatataattt ttcatgttcc ttttgttcca
    20641 ttcctttgct tcttttttct atctctcact tttagataaa ggtgttcttt aaagtatttt
    20701 tttgtccaga attgtcaaat catctgctaa tgtatactgg attggttgtg catttgggat
    20761 atcgatcgta tccatttgat cttcaggaac atcttctaaa tattttgtca gtgctcggat
    20821 gctattacca tgacccacaa ctagtaaatc ttgccgatct tttagttttg gtgctagatg
    20881 atcttgccat aagggaatga tccgattcac tgtcatttcc agactttctc cgtatgggat
    20941 caaatgcgga tccaagtgat catatctgcg gtcaaatcta ttttctgcgg ttaacggcgg
    21001 aacttccgta tatcctctgc gccatgtttt tacttgatct tttccgtata tttctttcat
    21061 cttatctttg tttttaccca caagatcccc ataatgccgt tcatttagac gccatgtttt
    21121 tattatagga agccataact gacccatttc ttctagtgcg acttgtccgg taagaatcgc
    21181 acgtttcaaa acagaggtat aaacggcatc gaattgaagt ccggcttcaa gcattttttg
    21241 accggcaact gctgcttgtt cttttccttt ttctgttaag gcgacatcta gccatcctgt
    21301 ccagtaattt tcaaaatttg cttcgctttc gccatgacgt aataatacta atttcattct
    21361 tttctccctt aaacattcgt ttcgaatttt ataccacatg ttttgcatgt cacacgtatt
    21421 cgtccttttc ctttaggtgc acgctgcttt tgatgacaat tcgggcactc aaaaaaagta
    21481 taactttttc gatccatttt gacttgcttt ttgtttttta tctgatgata tttttctttg
    21541 actctatcaa gatatttcaa ataacgttga ttctcattta gtctggggta gatttttttt
    21601 gataggaaac gaaatatcat ccatccgaaa aagacgatga acaacaattg tccgattcta
    21661 tagggcaacc aacgacttag caaactaaag acaagcgcag cgatcaatag accgcgattc
    21721 aactgatcaa atttcgcata tcttccctgc ataagtttgt gatagttctg ctgaaatcgt
    21781 attagccttt ctaaccatgt ttgcatcata ttatctcctc catcatctaa tatttattat
    21841 gacaaaaaaa tccaaaaaga aaaagcctta attggccttt tccattgcaa cttggtgaag
    21901 gacattccga tttctggtaa agtcaatcac aatcactttc tttttatctt ctctgttgac
    21961 tactcgaaat gtgtccgtca atggaagaaa cagcttctta gatggtagct gttgcatacg
    22021 tataaaacga tggatcatcg taatcaattg aggaaattga atagacgtgt gaagcagacc
    22081 tggatatttt ttcaacagct gtgtcgtttt gatcagattc cgttttttta ctgatcactt
    22141 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
    22201 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
    22261 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
    22321 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
    22381 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
    22441 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
    22501 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
    22561 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
    22621 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
    22681 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
    22741 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
    22801 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
    22861 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
    22921 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
    22981 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
    23041 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
    23101 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
    23161 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
    23221 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
    23281 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
    23341 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
    23401 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
    23461 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
    23521 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
    23581 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
    23641 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
    23701 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
    23761 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
    23821 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
    23881 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
    23941 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
    24001 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
    24061 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
    24121 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
    24181 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
    24241 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
    24301 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
    24361 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
    24421 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
    24481 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
    24541 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
    24601 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
    24661 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
    24721 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
    24781 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
    24841 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
    24901 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
    24961 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
    25021 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
    25081 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
    25141 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
    25201 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
    25261 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
    25321 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
    25381 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
    25441 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
    25501 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
    25561 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
    25621 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
    25681 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
    25741 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
    25801 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
    25861 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
    25921 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
    25981 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
    26041 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
    26101 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
    26161 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
    26221 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
    26281 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
    26341 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
    26401 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
    26461 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
    26521 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
    26581 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
    26641 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
    26701 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
    26761 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
    26821 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
    26881 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
    26941 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
    27001 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
    27061 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
    27121 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
    27181 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
    27241 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
    27301 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
    27361 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
    27421 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
    27481 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
    27541 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
    27601 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
    27661 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
    27721 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
    27781 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    27841 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    27901 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    27961 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    28021 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    28081 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    28141 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    28201 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    28261 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    28321 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    28381 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    28441 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    28501 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    28561 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    28621 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    28681 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    28741 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    28801 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    28861 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    28921 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    28981 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    29041 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    29101 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    29161 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    29221 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    29281 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    29341 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    29401 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    29461 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    29521 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    29581 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    29641 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    29701 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    29761 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    29821 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    29881 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    29941 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    30001 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    30061 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    30121 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    30181 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    30241 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    30301 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    30361 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    30421 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    30481 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    30541 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    30601 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    30661 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    30721 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    30781 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    30841 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    30901 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    30961 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    31021 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    31081 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    31141 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    31201 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    31261 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    31321 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    31381 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    31441 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    31501 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    31561 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    31621 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    31681 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    31741 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    31801 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    31861 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    31921 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    31981 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    32041 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    32101 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    32161 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    32221 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    32281 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    32341 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    32401 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    32461 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    32521 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    32581 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    32641 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    32701 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    32761 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    32821 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    32881 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    32941 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    33001 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    33061 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    33121 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    33181 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    33241 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    33301 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    33361 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    33421 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    33481 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    33541 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    33601 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    33661 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    33721 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    33781 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    33841 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    33901 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    33961 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    34021 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    34081 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    34141 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    34201 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    34261 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    34321 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    34381 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    34441 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    34501 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    34561 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    34621 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    34681 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    34741 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    34801 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    34861 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    34921 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    34981 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    35041 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    35101 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    35161 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    35221 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    35281 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    35341 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    35401 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    35461 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    35521 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    35581 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    35641 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    35701 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    35761 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    35821 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    35881 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    35941 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    36001 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    36061 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    36121 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    36181 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    36241 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    36301 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    36361 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    36421 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    36481 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    36541 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    36601 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    36661 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    36721 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    36781 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    36841 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    36901 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    36961 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    37021 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    37081 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    37141 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    37201 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    37261 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    37321 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    37381 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    37441 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    37501 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    37561 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    37621 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    37681 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    37741 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    37801 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    37861 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    37921 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    37981 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    38041 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    38101 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    38161 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    38221 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    38281 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    38341 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    38401 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    38461 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    38521 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    38581 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    38641 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    38701 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    38761 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    38821 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    38881 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    38941 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    39001 tttatgaaac ttttt
//
LOCUS       DAPCMU010000010        38599 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPCMU010000010 DAPCMU010000000
VERSION     DAPCMU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.010"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(211..2286)
                     /locus_tag="RD744_000433"
     CDS             complement(211..2286)
                     /locus_tag="RD744_000433"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDU0556465.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2288..3205)
                     /gene="glyQ"
                     /locus_tag="RD744_000434"
     CDS             complement(2288..3205)
                     /gene="glyQ"
                     /locus_tag="RD744_000434"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDU0556466.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3591..4403)
                     /gene="recO"
                     /locus_tag="RD744_000435"
     CDS             complement(3591..4403)
                     /gene="recO"
                     /locus_tag="RD744_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDU0556467.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4546..5445)
                     /gene="era"
                     /locus_tag="RD744_000436"
     CDS             complement(4546..5445)
                     /gene="era"
                     /locus_tag="RD744_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDU0556468.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5460..5855)
                     /locus_tag="RD744_000437"
     CDS             complement(5460..5855)
                     /locus_tag="RD744_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDU0556469.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5839..6315)
                     /gene="ybeY"
                     /locus_tag="RD744_000438"
     CDS             complement(5839..6315)
                     /gene="ybeY"
                     /locus_tag="RD744_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDU0556470.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6332..8524)
                     /locus_tag="RD744_000439"
     CDS             complement(6332..8524)
                     /locus_tag="RD744_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDU0556471.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8548..9519)
                     /locus_tag="RD744_000440"
     CDS             complement(8548..9519)
                     /locus_tag="RD744_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDU0556472.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10225..11604
                     /gene="brnQ"
                     /locus_tag="RD744_000441"
     CDS             10225..11604
                     /gene="brnQ"
                     /locus_tag="RD744_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDU0556473.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11760..12206)
                     /locus_tag="RD744_000442"
     CDS             complement(11760..12206)
                     /locus_tag="RD744_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDU0556474.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12233..12409)
                     /locus_tag="RD744_000443"
     CDS             complement(12233..12409)
                     /locus_tag="RD744_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDU0556475.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12571..12999)
                     /locus_tag="RD744_000444"
     CDS             complement(12571..12999)
                     /locus_tag="RD744_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDU0556476.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13091..13951
                     /locus_tag="RD744_000445"
     CDS             13091..13951
                     /locus_tag="RD744_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDU0556477.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14045..14395)
                     /locus_tag="RD744_000446"
     CDS             complement(14045..14395)
                     /locus_tag="RD744_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDU0556478.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14411..16138)
                     /locus_tag="RD744_000447"
     CDS             complement(14411..16138)
                     /locus_tag="RD744_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDU0556479.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(16352..17278)
                     /gene="trxB"
                     /locus_tag="RD744_000448"
     CDS             complement(16352..17278)
                     /gene="trxB"
                     /locus_tag="RD744_000448"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDU0556480.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17423..18859)
                     /locus_tag="RD744_000449"
     CDS             complement(17423..18859)
                     /locus_tag="RD744_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDU0556481.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     ENEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18856..20259)
                     /locus_tag="RD744_000450"
     CDS             complement(18856..20259)
                     /locus_tag="RD744_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDU0556482.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20489..21391
                     /locus_tag="RD744_000451"
     CDS             20489..21391
                     /locus_tag="RD744_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0556483.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21432..23318)
                     /locus_tag="RD744_000452"
                     /pseudo
     CDS             complement(21432..23318)
                     /locus_tag="RD744_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
     gene            complement(23411..24262)
                     /locus_tag="RD744_000453"
     CDS             complement(23411..24262)
                     /locus_tag="RD744_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0556484.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24588..26372)
                     /locus_tag="RD744_000454"
     CDS             complement(24588..26372)
                     /locus_tag="RD744_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556485.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(26372..28123)
                     /locus_tag="RD744_000455"
     CDS             complement(26372..28123)
                     /locus_tag="RD744_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0556486.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            complement(28265..28489)
                     /locus_tag="RD744_000456"
     CDS             complement(28265..28489)
                     /locus_tag="RD744_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDU0556487.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28740..30326
                     /locus_tag="RD744_000457"
     CDS             28740..30326
                     /locus_tag="RD744_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDU0556488.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(30392..32167)
                     /locus_tag="RD744_000458"
     CDS             complement(30392..32167)
                     /locus_tag="RD744_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDU0556489.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(32380..33282)
                     /locus_tag="RD744_000459"
     CDS             complement(32380..33282)
                     /locus_tag="RD744_000459"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDU0556490.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            33561..35906
                     /locus_tag="RD744_000460"
     CDS             33561..35906
                     /locus_tag="RD744_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDU0556491.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(36243..37244)
                     /gene="ccpA"
                     /locus_tag="RD744_000461"
     CDS             complement(36243..37244)
                     /gene="ccpA"
                     /locus_tag="RD744_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDU0556492.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            37454..38560
                     /locus_tag="RD744_000462"
     CDS             37454..38560
                     /locus_tag="RD744_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDU0556493.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT        11034 a         8190 c         6971 g        12404 t
ORIGIN      
        1 taatttaaaa gtaaataaaa cagtaacaag atcatgatta atcgttttca tttttatttc
       61 ctctcttttc atgagcattt tgaaaattcg aacaaagaat aaaataacaa aaattattaa
      121 aatagaatta ataacagcta tttatattta taacaaaaaa acagagacac agcacgtttc
      181 gtgcatgctc tctgtttttc aaacaaaaga ctattttgtg atgatttgat ccaagcttgc
      241 taatgacaaa gccatggcag ctatttgact aagttgtgtt agtcggttct tacgtactgc
      301 tggttcatca gccatgacca tcgtttgatc aaaataattt tcgatcagtg gacgaagagc
      361 gacaagctgg tcgtagtttt cgtggatcgt ttggtcagca aaacttgctt tcattgcttc
      421 tactgctttg tgtaattctt tctctgcgtc gttttcgaac agctcaggat ctacttctga
      481 ttgtttttca agctgcgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccattga
      541 aggtttgaag tcttcatctg caagatgttg tttgaacaat tgtgctgctg caaatacttt
      601 tgtcagatct ttttgttccg cattcaaaac agcatcgatg acatcatgac gaatattctt
      661 cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacttgtt gttgcccttc
      721 tgataaacca atgccgaaac gatccgtatc tttactgata acatcgctaa tcgtttcttg
      781 cagaacagac aaaggaaagg cccatttttt cgattcgatg atccgaacga caccgtatgc
      841 ttggcgacgt aaggcataag gatcgtttga accgcttggg atcatcccaa cagcaaagaa
      901 agaaaagatg ctatctaatt tatctgcaat cgctaaaaca gaacctactg tagactgagg
      961 tagctctcct tcactggata taggcatgta atgctcacga atcgctgtcg ctacagcagg
     1021 tttctcgcct ttcagcagag catatttttc gcccatgatc ccttgcagtt caggaaattc
     1081 ccctaccatg ttggttacta gatcaaattt atagatttca gcagctcgtt gcagatccat
     1141 caattcttcc tcagatagtc ctacttgttc gccaatgata cgggcaatga cttgtacccg
     1201 ttgcattttc tcatagatac taccgatttt ttcatggaaa gtaacagttt ttagtttttc
     1261 aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa
     1321 acgagccgtc aatacttttt gattgcctcg aaccacattt tccaatttta cccgatcacc
     1381 attacgagca gaaataaaat gaggaagcag cataccttga tcattccgta cctcgaaata
     1441 acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagaaa gatatttttc
     1501 ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt cttctagtaa
     1561 atcttcgtct agatcaataa cccaatgatt tttttcggct aattcattgg cttgtgtaac
     1621 gatcatttct tgtctttttt gcggagacgc aatgacgaat tgttcattca atttttcttc
     1681 atattctttt gcatgcgcaa atgtcacatc ttcgcctaag aaacgatgac ctcgagaggc
     1741 tctgcctgtt ttgacatcca agatttcgaa tgggattact tcgtcatcta acaatgccac
     1801 gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca tcgttacagg
     1861 gaaagtcagg cttttaatga catctgctaa acctgtcaat acttctttgg attccttacc
     1921 atgagtgtat ttcgttacat agacatattc cacacctttt aattctctaa aagtgatagc
     1981 gtctgtcgtc agaccttgtc cgcggacaaa tccttccgct gctttcgtcc agtttccatc
     2041 tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt ccgcttgtcg
     2101 ttctggaatg tttgttactt tgattgctaa acggcgcggt gtggaaaatg tttcgatcgt
     2161 atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat
     2221 gcttggtgtg acaacatgag caggcatttc ttccagcccg atttcaagta ataagtcttt
     2281 tgccatctta ttcagcctcc ttcacgtttt ctggatttgc tgtgttttta ttcaacaaag
     2341 ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc
     2401 ggatgcgaga aagataaccg gcacgctctg ttacggatac tgcaccgcgg gcgtctaata
     2461 agttgaatgt atggctacat ttcaagatat aatcatacgc cggatgaaca agattttcat
     2521 cgatacagcg tttagcttct ttttcaaaac gatcaaagtt gcttagtagc aagtcttggt
     2581 cgcttacttc aaaagaatat ttagaatgtt catattctgg ttgtttgaag atctcgccat
     2641 atttcacgcc atcagcccat tctaaatcat agacactttc aacttcttgg atataagaag
     2701 caagacgttc taatccataa gtgatctcag aagttactgg tttacaaggc agtccaccaa
     2761 cttgttggaa gtacgtgaat tgtgtgattt ccattccgtc aagccatact tcccaaccca
     2821 caccggcaca gcccatggat gggttctccc agttgtcttc aacaaaacgg atgtcgtgtt
     2881 ctagtggatc gattcctaaa aggcgcaagc tttctaaata aagctcttgg atattctcag
     2941 gtgaaggttt catcacgact tggaattgat gatgctgata caaacggttt gggttttcgc
     3001 cataacgtcc atcagctggt cggcgagatg gctctacgta agcagcattc catggttctg
     3061 gtccgattgc ccgtaaaaag gtatatgggc tcatcgtacc tgctcctttt tctgtgtcat
     3121 acgcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat
     3181 cttggacggt caatttttta ctcatctgtt ttgccccttt caattttaaa gcgttgctgt
     3241 caaataagaa tacaggaaag aaaacgacaa aaaatcccta tgtctgatta cagacatagg
     3301 gacgaataat tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg
     3361 tactcgcaag tgcctcttca aaaatcgttt gggtccggct cccactctcc cgaactcgct
     3421 aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca gttttcttgt
     3481 ttctataata gatcctctca gtgaacgatc tatttgatct acggttaata atagacggaa
     3541 ctccctgtgt ttgtcaaatg gttttgattt tttgttgtat gtttcattta ctattcttct
     3601 tttttaggta gatctttttc agacatgtct ttctctgacg tctttaatac gtgtgcccaa
     3661 gacttcattt gatcaatgaa ctttttgctt ttcaaatgga taccgacata ttcatcatac
     3721 agctggtcga tgagttcacg gattgcttgt ttggtttctt ctttcagatg gattccactg
     3781 attttatcat aagaaatagc tgaaaacatc cgaacgaaat aaacagctcg aggatctgca
     3841 tgataacggt gatcatccat cgaccagtgt ctttcgcata tcacaccacc atatttggaa
     3901 gaatagtcaa attttccttt tgtttcccca catacgacgc agtgtgtcca gattggtgca
     3961 attccgaagc gttgcagtat ttggacttca aaaatattcg tgatgatttc tgcatcatct
     4021 ccttggtcca tcagttgcag tgcttgttgc gtaaactgga acaaatgcgg gtcgtactga
     4081 tgatcttcga ttgccgcatc tactagattc aataaatatg tgccatagcc gctcaaaaag
     4141 atgtcttctt ggatgtgtcg aaacgcgtgg acatctttga cactgcttaa aaatgacaaa
     4201 ccttcacttc gaaaattccc gatataaact gcttcagtaa aagggagcaa agccggagct
     4261 aagggctgat tttgccgatg gatccctttg atataaaaca tcattttgcc agaagattcc
     4321 gtaaagattt tgactaattt atctttttct ttgtaattac ggctagataa aatcaagcct
     4381 tttgattctg caagctggct catattctcc ctcctaaaaa ataaatctgg aaaagcagat
     4441 tctgccgatc cagatcattt atgttgcgat cttccgcagc ttttctcaac acgcggattt
     4501 ttatccagtt tatttctata cctaacttac aagaagctca gaatatcaat attcgtcttt
     4561 tcggtagccg aagtcttgca gatagacttt tttgtctcgc cagtcttttt gaactttgac
     4621 ccaaagttct agaaagactt tgtctcctaa aagattttcg atatcttttc tcgctttcgt
     4681 tcctacttgc ttcagcattt ttccgccttt gccgataatg atcccttttt ggctatctct
     4741 ttcgacaata atcgtagcct ggatatggat tttatcattc tcgtttcgct tcatcgaatc
     4801 aacaacgact gcgacagaat gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg
     4861 gatcaattca gaaacgatga agtattcagg atgatcagtc acttgatcat ctggaaaata
     4921 ttgtgggccc tctggcattt cactgactaa tacatccatc aaacgttcga cgttgtttcc
     4981 ttctgtagca gaaattggaa tcacttgtgc aaacggcatc tgcgttgaat aatcttcgat
     5041 gattcccaac aactcgtctg gatggatttt atcgatttta ttgataatca gataaacagg
     5101 agaatgcacg ttttttagac gttcaataat aaaatcgtct ccccgaccgc gtttttgatc
     5161 agcgctgatc ataaataaga ccgcgtccac ttctttcaac gcactgtaag ctgcttctac
     5221 cataaaatct cctaaacgat gctttggttt atggattcct ggtgtgtcaa taaaaatcaa
     5281 ctgagcttct ggtgtagtat atactccttg gattttattt ctagttgtct gcgccttatc
     5341 actcatgatg gcaatttttt gacccacgat tcgattcaat aatgtggatt ttcctacatt
     5401 cgggcggccg acgattgcga caaaccctga tttatgttct ggcatatacg ttctcctttt
     5461 taattaaaca acaaatgata tattttaggg acaaataaaa tcaacccaac gatgacggaa
     5521 aaacaagcgg tcactaaaac tgctccagct gccatatctt tgattttttt cccaatcgga
     5581 tgaaaatgaa aatccgtaaa catgtccacg acattttcaa aaacagtatt gatgatctct
     5641 actatccata cgagaaaaac agctaaaagc agccataacc attctatccg atcaagctgg
     5701 aaaatcacgc ccataaacaa agcgaggatt ccgaacgcaa catgtttcct catgttgcgt
     5761 tcttcctcaa aaacggtttt gactccttgg acggcaaatt ccacggacat caggaaatgt
     5821 ttgtttttct ctacgttttt atcttttaag tccataggca tctaagatct ctttctgtaa
     5881 accaaacatt tctttctcat cctctggtgt catatggtca tacccgttga tatgcaagaa
     5941 tccatgaacc gctaaaaagc caagttcccg atcatacgaa tggccgtaag cttctgcttg
     6001 ctctgcagca cgttcagtag agatcatgat gtctcccagt acacgaggca atggattctc
     6061 tccatcttca aaaatcactg ggatttcgtc ttcgccctct tcttccaagg caaaactgat
     6121 gacgtctgtc ggtgcatctt ttccacggta atcacgattg attacttgga tcgctgcatt
     6181 gtccatgaaa gtaacagaca tttctgtttc ttctggcaac tctaaatatg atgctgcaaa
     6241 ttgcagcaag tcgtcgattt ctttcatttt ctcttctgag acttggtttg tttcatccat
     6301 gaaagtaata tccattatac atcgcttctt tctattcttc ttgttcttct tttatcttct
     6361 caacttccga tttcatttta ggatatttga tccgagaatg gaaggtactg cttaaaccgc
     6421 tgatcaatga tttttctact ttacggattt cttttaacgt caatccactg tcgtccaatt
     6481 gtccatcgga aatacgctct tcaataagat tatggacaaa actttcgatt ttttcgctcg
     6541 ttggatggtc cattgctcgt actgccgctt cacaactgtc agctatattg acgattcctg
     6601 cttcacggct ttgaggtttt ggtccaggat aacggaattg ctcttccgtg acttccgggt
     6661 tgcgttcctt tgccttaacg tagaaataac gcatcagcgt ggttccatga tgctgtctgc
     6721 aaatatcgat caccatttgc ggcatattat aatcttccaa tattttggca ccatctgtca
     6781 catgtccaaa gataatatgt ttactgtctt ctggcaataa gaaattgtgc ggattttccg
     6841 ctcctgaagg caagttctca acgaaaaagc tggcgtgttt gatcttgccg atatcatgat
     6901 aatagcaagc tactcgagtt agtaaagaac gtccaccgat ttctgctaca gcattagcac
     6961 ttagattggc taccatcatg ctatggtgat acgtgcctgg tgcttcttcc agcaactgtt
     7021 tcaataaagg atgattagga ttgctgagtt catttaatac gatcacacta tcatcattga
     7081 ccatcaattc aatatagggg tgcagaccta tcgtcagcaa gaatgagaac agtgcacctg
     7141 tgaatccgca tactagcgtt aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga
     7201 taatcaaaat gacatccatc atcacaggaa aaataatgac ccacatagca gcggataacc
     7261 cttgttctga gatccgtttg cgtctgacga tcgttgccat gagaccagaa aatacatagg
     7321 acatcaaaat aactgtcaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg
     7381 atactgcttg aaaaatacca gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa
     7441 caagtggagc aaaagccgcg gggtaaaaca gcggaatata agcagcttgt tctgtttgga
     7501 agagttggaa aaatttcatt agtacgacgc ttactgacat tgccgtcacg tagaaaagta
     7561 cgtattttgt tctttctcct gcattttgga attgaagtga attataaatc agtacaccga
     7621 tttgaagcaa aacagctaaa accatggcaa tgagtgggaa aatagaagtc gtttgattcg
     7681 tcaatcctag taattccaat tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa
     7741 caataatttc tccttggaag atcatcactg gttgtacagc ttctcgtgca gattgtttta
     7801 attcttctgt ctttttctca ttcaatacat cattaactac aatgccttga tccactaagt
     7861 agcgaatcat ttgttgctgc tctgttgtga cattcaaata ttgaatctga tcttctgctt
     7921 cttgtttaaa agcttccaat tcgctttcac gaacttgttt actcatctgt tcgtcaatga
     7981 gcttcaaact ttcatcccta accgtgtgaa tttccgtttc agtcatttca aatatcgtcg
     8041 aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa
     8101 aatttttctt cagtgtagca atccgctctt ctgtagtagg tttcgggata ttttcatctt
     8161 ccttcgcctt atctaccttg tcattataag atttgttgat ggtatcattc gtcttatcga
     8221 tcaattcgaa taattgcttg atccgattgt tctggtcatc tgccagatct ttttgataag
     8281 tatattctgg agtcactgct tctgctgcca gttttcttct ttgttcggtt tcttccgtgt
     8341 tctcaatcgt tttattcgca cggatactct cttctgctac ttggccttct ctgaaatcat
     8401 tggccttttg tttgacacta ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca
     8461 gaaggaaagg caatgctgcc tttccgagtt ttttttgtag tttctgcagt gttttattca
     8521 acatgtgctt atcctcctaa gcaaagctta ttcttgatga agatccgcct tttcataagc
     8581 tttgatgatt tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa
     8641 atcgattttt ttgatttgct ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc
     8701 acgaggcaag tctatctgac ttgtatcccc attgacaatc atttttgatt gataaccgag
     8761 acgggtcaag aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac
     8821 gaatgcatca tccaatgtcc gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc
     8881 tcgttccatc aatcggttcg tatgatccaa tccaaaaatt tggtataaag catcataaac
     8941 aggccgcaga taaggatcca ctttttcttt cagatccccg ggtagaaagc ctaggttttc
     9001 ccctgcctcc acagcgggcc gcgtcaaaat gattttttgc acttcgcctt tttttagggc
     9061 agcaattgct aatactacgg caagaaacgt tttccctgtt ccggctggcc ctacaccaaa
     9121 tacgatgtca tgtttggcta ccgattcaac gtatttcttt tgaccgctgt tttttacccg
     9181 tataggtttt ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc
     9241 taatgtacct ttttgcccca tcttcaatgc cgtcacaaca tctggcgtac ttacgtggat
     9301 tcctcgtttg atcaattcct gtaatgtccg aatgactgag gcagccaagg aaatctggga
     9361 ttgctcacca atgatctgaa tgatttctcc acgtgtatgg atggttgttt ccgttgtttc
     9421 ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc
     9481 tgtcaatcta atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt
     9541 cgattcattc tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg
     9601 ttgtaaaaga gtcgtttaac catacgtacc aagaaacgtt aaaaaatagc cgtttccatt
     9661 cgctaaaggt ctcgttttga taaaaagaag ctgagaatgt aagttatatg aaagtggaga
     9721 acaaaatatt ccgtttcatt cagcaggtat tctctaggta tttttcttgt ttcaaaaaaa
     9781 gaaaacaaaa tagttaaaat attcttgcat ttcaaaaaaa acaggtgtat tattacgtca
     9841 acaaataaac atacagcaaa gcgtcgagga aaacagtagg ctattcaacg atttattccc
     9901 agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc
     9961 aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat
    10021 caaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga
    10081 atctagagcc cttttcccaa gtggaaaagg gcttttttta tattttaagg tggtggtttt
    10141 catggaatct gatattagtg gcattactaa ttgctgcaac gtttatatcg tcaatcaaaa
    10201 tgataattat tggaggaaaa atttatggaa gaaacaaaaa cttattcatg gaaacaacta
    10261 ttgatggtca gttcacttat cttcgggatg ttcttcgggt caggtaatct aatctttcct
    10321 gtccacttag gtcaaatggc tggcgcaaac tgggtcagtg ctggcgtagg atttgccatc
    10381 tcgggcgctc ttttcccatt actagctatc ttagctgtag tagtgacaaa aagtgatggt
    10441 ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg ccttgttctt aatccttgtt
    10501 catttgacga tcggaccatt tttcggtaca ccacggacag ctgctactgc ttttgaaatg
    10561 gctgctaaac catttttgcc tgaaaaatat aattcattag gtatgttttt attctctgct
    10621 cttttctttt tacttgctta tttccttact gtccatccca cacggttggt caaatacgtc
    10681 ggaaagtatc taaactcctt gtttcttgca ttgttagcaa tcatttttgt cgtttcttta
    10741 ttccacccaa tgggaaatct aaatcaatct gctgcagata cttaccaaac aaacgcttta
    10801 acaaatggtg tcctagaagg ttataacacg gttgatgcag tagccttgct tgccttaagt
    10861 gtcacttttg tccatgcagt ccgtgacctt ggtttcaaag ataaaaaagt cacagaatta
    10921 acagcaaaag ctggtagcct gagcgttgcg ctagaagtca tgatctattt tggtcttgtt
    10981 cttctcggtg cgatgagttt gaataagatg tctttatcag aaaatggcgg aactgcactt
    11041 tctcagatca ctaattttta tttaggtaac ttcggcgcta gcttcttagg agtgatggtc
    11101 acattaggtg tcttcactac cgctatggga ctcgttgttt cttttgcgca agatttccat
    11161 aaactgttcc ctaaagtcag ttatatgaca tggctgcggt taacgacttt cgtctctttt
    11221 gtcgttgcaa atgctggttt agacaatatc atccaatggt ctttaccagt attgatgctt
    11281 ttgtatccat tgtcgcttgc gttgatcttg ctttccttaa cagcaaaatt tttccaaaag
    11341 acaccatttg tctatcaagt aacgatgttg tttgctgctg ttcctgccgt attggatatg
    11401 ctggcaaatt cgcctgcttt agtgagtcaa caaagagttg tagcaagcat gctcgaattt
    11461 tatcatcatc acgttccttt tgctgcatta ggacttggct ggatggtccc aacgttactt
    11521 ggttacgctg gcagcctact gttttattac gcctatcgcc tatcagggta taaacaagaa
    11581 gcaaatgaac taccagaaga ataaccaaat caaacacaat ttaacataag aaatattaaa
    11641 caggaaaaaa ccagctagat gaaagaatcc tattctttcg tctagctatt tttttgttct
    11701 cttttttacg cgcacaaaaa aactgcggac aatgattgtc cgcagtttaa atgataaaat
    11761 taagattgta atagttcttt cacaatggca tttacttggt ttccatctgc ttttcctttg
    11821 actttaggca tgatcgcacc catgactttg ccaaattctt tcattgatga agcaccagtt
    11881 tgtgtgattg cttcttgaac aagttgtcga atttcttcgt cggaaagttg ttccggcatg
    11941 tatttttcga ctatcacaat ttcacttttt actttttcag ctaaatcgtc acgtcctgct
    12001 tcttcaaact catgcaaaga atcacgtcgc tgtttcattt cgcgagacaa caccgttaat
    12061 tcttcttctt ctgtcaagtc gtgaccttct ttaatctgct cgttttgtat agatgacttg
    12121 atcattcgga tgacttgtaa agattcctta tctttcgctt tcatcgctgt tttcatgtca
    12181 tcgtttaatc tacttagtag tgacacacta ccaactccaa ttcatcacag aactagaatt
    12241 ttttgcgttt tctagctgct tcagattttt tcttgcgtct tacacttggt ttttcgtaga
    12301 attcacgttt acgagattct tgtaaagtac ccgcttttga cacggaacgt ttgaagcgac
    12361 gaagagcatc gtcaagtgat tcgtttttgc gaacgactgt ttttgacatg ttaattccct
    12421 ccctccgagc ttaaaaagta accgtaattg ttattgaaat atatctcaaa aaacaagact
    12481 gtcctgtaaa tattatatct aatgctgttt tgaccgtcaa ggaaatcttg ctttttttta
    12541 aaattatcaa atgttatttt atttaaatcc ttattgacaa ttttcacatc ttccgaaaat
    12601 ctcgaatcta tgcccttcga tcgtacatcc accgagctgt tcttcgaaat aattcattgg
    12661 acacatatgg atttctttcg ttgttccgca caccgtacag ataaagtgat ggtgatggcc
    12721 gatttcccct tgacagcaat gaaaacgaaa cttcatctct ccatttaaat cggtttcttc
    12781 cagtaaatcg atttctgcaa aatcatggag atttcgataa atcgtatcgt aacttactcc
    12841 tttgaattcc tgattcatat agtcaaaaac ttctttagcg gatacgtagc gattgcgctt
    12901 cactaaataa gttaacaatg cttctctttt tttagtatat ttaaaaccgt tatttttcaa
    12961 tgtttctagt gattgttcaa tcaatgtctg ttttttcatg acttattcct ccaaatcaaa
    13021 atgattacga agtacacatt ctagagtatg gtataataaa cataaaaaaa atgcaagtga
    13081 ggttttatcc atgtcttccg attctacaga aatcgttact ttttttgtca tctctgactc
    13141 tgctggagaa actgcgacaa aattagcgca agccactatg gcccaatatc ctaccgttga
    13201 gttcaattta ttccgcagaa catttgtgac agatgagaaa acattgatgc aggcactgca
    13261 agatgcacta aaagaaaaag cccttgtcct gcacacattg atcaatcaag aattgatcga
    13321 gatgacgcgt tctttttgtg aagaacatca attatttagt tttgatgtga tgactccgcc
    13381 gatatcagaa atcgagcgct taactggtat caagcccatt cggcaacctg gcgcccttca
    13441 tttattgaat gagaactatt tcaaacggat caaagcaatg gaatttgctg taaaatacga
    13501 tgacgggaaa gatcctcgtg ggtttctaga agcagatgtc gttttgctag gcgtatccag
    13561 aacttccaaa acgcctttga gcttgttttt agctaacaaa aatttaaagg ttgccaatct
    13621 acctttgatc ccacaagcac atattcccaa acagctttgg gagatcgatc ctaaaaaaat
    13681 cgtgggactg acgaataacc cagatatttt aaacaatatc cgtaaagaac ggatgcgctc
    13741 ttacggatta aatcctgata ctgcttattc cgatatcgaa aaaatacgag ctgaactcga
    13801 ttttgcgaat gatctttatg aaaaattggg ctgcgtagta atcaatgtcg cttctttatc
    13861 tatcgaagaa acagcttcac ttattttgaa tgctttagac ctagaagatc atagttatta
    13921 tggaactgaa accaccgaag aaacagacta acagtttttc ttgtcttttt cttaaaaaaa
    13981 ctggactgtg tcagcgaaga agattcgttg atacagtcca gtttgtctct atttgcacct
    14041 atcgttattg atgttccaca tgtttgagcg tctggttcaa aatatccagt acgtgagaat
    14101 catcaagact atacaaaatc gcttttccct cacgatggga cttgacgaca tggttttctc
    14161 ttaataaacg cagttgatgc gatacagcag attgttccat ttctactgct tgggtgattg
    14221 ctgtcacatt ttgttccccg tctttcagat acagcagaat tttgagacga gtggggtcac
    14281 tcaacatttt gaatagttgg ctgactttct ctacttttgt tttttgcgtg tctggcaatc
    14341 cattcacctc caacttgaaa aaagactgac ttctaaagtt taccactttt tccatcagtc
    14401 tcaagttttt ttattcttct gtcatttttt tgataatcgc ttcataagcg gctacttttt
    14461 cagctgcgtc ttctgaagag tttcctttta caccgatata aaatttgatt ttcggctcag
    14521 ttcctgaagg acggatagcg atccagccat tttcttccag cgtatacttc aatacgtcag
    14581 atggcggtgt cgttagtttt tctacgtttc cttcacggtc tgttctcgtc aactctttga
    14641 agtcttctgt ttggatgact tttgctccgg caaattctgt cggtgcttct tcacggaatt
    14701 tttccatcaa agcagagatt tttgcagaac cttcttgtcc gctcattgtc acagaaatcg
    14761 ttttttctgc gtagtagcca tatgtttcaa aaatcgtttg caagccgtca tacaacgttt
    14821 tgccttgttt tttatagtaa gctgctactt ctgctaataa gagcaatgct tgaatcgcgt
    14881 ctttgtcgcg aacgaaaggt ttaactaaat aaccgtaact ttcttcgaat ccgaacataa
    14941 aagtatggga atgatcttct tcaaattgtt gaattttttc agcgatgaat ttgaatcctg
    15001 tcaatacatt gaacatggta gccccatagt gttccgcaat tgctgtaggc aattcgctag
    15061 aaacgattga tttcaatacc gctgcatttt caggtaaagt gcctcgcgat tggtgtgctt
    15121 ccaaaatata ttgagcaaac agggctccga tttggttccc tgtcaatact tcataggagc
    15181 catttggcaa acgaacggct gcgcctaaac ggtctgcatc aggatctgtt gcaatcagta
    15241 agtcagcgcc ttctttctcg ccaagtcgca tcgcatactc gaaagcagag tgttcttctg
    15301 gattcggtga tttgactgta gtgaaatccg ggtctgcgac agcttgttct ggaactagta
    15361 cgaattgttc gaatccagct tgtttcaacg cacgttcgcc caacattttt cctgttccgt
    15421 gaagtggtgt gtacacaagt ttcaaatctt tacccattgt ttccaccaat tcatgatcaa
    15481 tcgttactgc tttgatttct tctagataag cggcatccac ttcttctccg atgatcgtga
    15541 tcaatccgct atgttcagct tcgtcgtcag ataatacttc aacttttaac ggattgctga
    15601 cttcacggac atatttcgtc aatgcatctg cgtctgcagg aggcatttgt cccccatctt
    15661 ctccgtagac tttatatccg ttgtaagctg ctggattgtg ggatgcagtt accatgattc
    15721 ccgcaaacgt cttcaaatga cggactgcaa aagaaagctc tggtgttgga cgtaaacttt
    15781 cgaatacata agaagggata ttgtgatgtg ctaatgtttt ggctgcttcc atggcaaatt
    15841 ctggtgattg atggcgtgag tcataagcga tagcgacacc tcggcgtttt gtttcttcac
    15901 cttgtgtatc catgaaacgt gcaagtcctt ctgttgcttg tcttaccgta tagatgttca
    15961 ttcgattgat cccaggtcct aagattcccc gcatgcctgc agtaccgaat tccaacggtg
    16021 cataaaaagc atcttcaagt ttttcaggat cttcacccag atcaattaat tgttttttta
    16081 agttttcttc taaattttct tcatgtaccc attgttcata cacttgttcc caagacatga
    16141 aaataacacc tctttctgaa atatggttct taataagtat agcattcact tattagatat
    16201 gaaagtacga ggtctaaaaa acgcttaaat caagaaagga ttcttttatc aagcacggtt
    16261 gcaagtattc gcataaatcg gattcagtag atttcacgcg aaaaaaaaga gtaaaagaac
    16321 aattgaaacc gttcttccac tctttttaaa cttatgcagt tgtttcttct aaagattcca
    16381 agtatttata cacttgttgt ccagcgatcc caccatcacc aacagcagta gtgatttgtc
    16441 gcaaatcttt ttcacgaaca tctccgatgg caaaaacgcc tggtatattc gtcttcattt
    16501 cgttgtctgt tggaatccag ccagcttcgt tcgtcaaccc tgcttctcta aatggtgcag
    16561 ttaatggatc cagtccaaca tagatgaata ctccgtctgc tgccaaatca aactcattgc
    16621 cggttttgac attacgtgct ttcacacctg tcactaccat ttcattccca acgatttcgt
    16681 caacgactgt atcccacata aaggaaattt tttcattcgc aaatgcacgg tcttggatga
    16741 tcttttgtgc acgtaattca tctctacggt ggacgatgac tacttcagaa gcaaattgtg
    16801 tcaaatagat ggcttcttct actgctgagt ctcctcctcc gatcactact agacgtttgt
    16861 tacggaaaaa cgcaccatca caaactgcac agtaagaaac gccacgtcca gcaaattctt
    16921 cctcgccagg tacgcctaat ttacggtgga tacagcctgt tgcgataatg actgtttttc
    16981 cttcgtaagt tttatcttca caaataactt ctttgtggct gccgtgatcc ttgatatctt
    17041 gcacgattcc ataagcattt tctgtcccaa atcttgaaac accttcatac atcttataag
    17101 ctagatcagg acccatgatt gaatcgaaac caggatagtt ttcgatttct gccgtattgt
    17161 tcatttggcc gccaggggcg ccttgttcga tcatcaatac ggataagttt gaacgtgaag
    17221 catataaagc agcagtcatc ccagcaggac ctgcgccaat aacgattaca tcatacatat
    17281 gtagattctc ctttacttaa tcaagtaagc atactttaca gccaatgcga aaaaaagtct
    17341 attgttctgc ttgcgtttct cgaatagctt tattatactc gaaaaatccg ctgaaaaaca
    17401 atagactttt acaaatcatt gatcaatcat tcgaaagatt ttctccattt gaatcaatta
    17461 cttttttata ccagttgaat gatttcttct tcgaacgggc taatgtgcca tttccttcgt
    17521 tgtcacgatc gacataaatg aatccgtacc ttttcttcat ctcacctgta ccagctgata
    17581 ctaaatcaat acatccccaa gtcgtatagc ctaatagatc gactccatct aattctactg
    17641 tatctttcat tgcctggata tgtgctgcca gatagtcgat tcgatagtca tcttccacat
    17701 aaccattctc atcgggcgta tcgactgcac ctaagccgtt ttctacaata aatagtggtt
    17761 tttgataacg atcataaata tcattcatcg ttattctcaa gcctaatgga tcaatttgcc
    17821 aaccccattc acttgcttcc agatatggat ttttgacaga agcaaagata tttcctgcag
    17881 tctgttccaa gagttcgttg tcagtagtcg aaactcgaga agaataataa gaaaaggaga
    17941 taaaatcaac agtatgttct ttcagcaatg cggcatcccc aggttccatt ttgatctgaa
    18001 tgccatttcg ttccatttct ttcaatgcat aagcgggata ttctcctcgt gactggacat
    18061 caatgaagaa ataattttct cgatctgatt ttctagaagc ccaaacatct tcaggattgc
    18121 aagtgtatgg ataatatgct cctgctgcca gcatacagcc tactttgttc tccggatcta
    18181 cttcatgagc aatcttcgtt gcgatggcac ttgcaaccaa ctcatgatgt gccgcttgat
    18241 atttgatttg ttcttcattt tctccttctt caaaatacag ccctgcgccc atgaatggtg
    18301 catgaaggat catattgatc tcattgaatg tcaaccaata tttgacaagt cctttatagc
    18361 gattgaagat gactcggcac agattttcat aaaaaccaac gagttctcta gaacgccacg
    18421 caccatattc tttgatcaaa tgcatcggac aatcaaaatg tgtaatcgtc acaagcggct
    18481 cgatcccgta cttttgacat tctttaaaga catcctcata aaatttcaaa ccagattcat
    18541 ttggctcatt ttcatcgccc attggaaaaa tacgactcca agcaatggac atccgataag
    18601 tcttgaaccc catttctgca aacaaagcaa tatcttcctt ataatgatga tacatatcga
    18661 tcgcaatttt tgctggataa aaatgatctt tatcaaattc aaacattttc ttttttccag
    18721 taatgacagg aaaacggtct tctccgattg gcacaacatc tacgttcgct aacccacgtc
    18781 cgccttcatc ataaccaccc tcgcattgat tggctgctgt agcaccgccc cataaaaaat
    18841 cttttctgaa tcccatcata cttcctccat tacatgtgta ttgcttgaca attctcttgc
    18901 cagttcatct tggacttcga ttccttctgc ttttaaccgg ctgattgttt ccttgaattg
    18961 cggtaaatac tgctgatgtg caataaacaa ctcatccatg atccgttttg ctgtctttcc
    19021 tgatcgtata agcggattga tcgtaaacgc ttgtaaagct gttccataat caccagtcac
    19081 ggctgcttcg atcgtcagca attccatatt cttcattact tgcaaccagc ctctttcagc
    19141 agcaggaagg gaaccaaacg ctacatttcg tgcgccttgt gcgccaacat aggcagtgac
    19201 ttctactaca caatctgccg gcagatcagg aacagctcca tcattctttg tagacaccac
    19261 gatctgtgtt tctttatttc catagatcga agcaatcgtt tcacaggcag catctgaata
    19321 ataagcacca cctcgttttt gcaattgttc aggtttatga tccaattctg gatctttgta
    19381 taattcaaac agctctgctt ctgtttcttt cacttgttgc gctcttgtac caatcgtttg
    19441 atattcttct aaagcatgtt ccaacatttc ttcttcacgg tagtaatagc ggtggtagcc
    19501 acaagggatc atgttcattt gttttaattg ttctttgaaa aacgggatgt caaagatatt
    19561 tttcggcaat ccattgtctt catcgtataa acgatcaatc aaatctgctg tgcggtcatt
    19621 tcctttatta tctgctactt tgtgccaatg gaagtgattt agaccggcaa acttgtagat
    19681 cagctcttct tttttcttgc caagcattgc tggttctgtc atcgtcgcca ttactggcac
    19741 attgcataat ccgatcactt tatcccattt gccatatctg ataactgctt ctgtcaccat
    19801 cccgcttggg ttcgcgaaat tgatcagcca agcatttgga cataaacgct tcatgtcttc
    19861 aacgattgcc aaaaccactg gaacggtacg aaaagctttg aatatcccgc cagcaccatt
    19921 tgtttcttgc ccaatcatcc cataataact agggatccgc tcgtctttga ttcgtgcatc
    19981 taattgaccg acacgaaact gagtcgtaac aaaatccgca tctttcaatg cttcttcccg
    20041 atccaaagtt gcatagatct tgacttcata cggagaagca tcccacatac gctgagacat
    20101 ctgactgacg atctcaagct tttcttttcc ttcttctaca tctacgagcc agatttctcg
    20161 tatcggtaat tcctcatatc ttttgataaa tccttccatc aactcggggg tgtaactgct
    20221 gcctccacca atcgtagcga ttttgattcc ttttgccatg ttctcactcc tttgcttttt
    20281 acacttttat tatctttttt gtaaacaaaa caaacaatag aaattcgttt ctttccagta
    20341 acatttacaa aagttagtgt tttctactgg atttttgtaa atatcttgtc aaaaaatgac
    20401 tttattaagc tatataacat aaatccgttt acaaatcatt ttcaaaaaag ttataattta
    20461 cgtaaaaaaa ctagaagaag gtatctacat gttattacgt gaaaaaatca aagaaatctc
    20521 cttttctcct tcagaaaatg aagtgattca gtttctctta agcgattctc cagcacttgg
    20581 cgaaatgacc atccaagaga ttgctgagcg atgttatgtc cacccgtcca cactcatccg
    20641 tgtagcaaaa aaaatgggtt ttgatggctg ggtcagtttt cgtaatgctt ttttagctga
    20701 aacagcctat cttgaaggga attttcaaga cgtagatgcg aatcttcctt tttccaccac
    20761 agacggtatt atgacgatcg caaataaaat ggcttcttta gaacagatga cgattcaaga
    20821 tacactttct ctcattcatc atgaccaact gcaaaaagcc aaacaactaa tgcttcaagc
    20881 aaaaaaaatc gtgattttcg ccagtaactc gaatactttg atttctcaag atttcatgct
    20941 gaaaatgaag cgcatcaaaa tggatgtcac gattgtcacc acttttggtg aatcgttcta
    21001 tgaagcgtac aattgcacga gtgatatgtg cgggattctg atttcttata cgggtgaaaa
    21061 caatgtgatc cttcgtaccg cagaaatatt gaaacaaaat catgttccaa ttttagcgtt
    21121 gaccagtatc ggtgattcaa cgctatcctc aatgagtaca gccgctttgc gacttacgac
    21181 acgcgaacga ctgtattcaa aaattggtaa ttttacgatc aatacatcga tttgttatct
    21241 tctagacgtg ttatatagtt gtgtttttgc agaaaattat caacagaatt tagtccatct
    21301 aatttctgta ggacaaaaat ttgataaacg ccctatcagc tcagccatca tgaaagagcc
    21361 tttcaatttt gaaaataaag aagtggacta aaaagaatag ttaagtctat tccattcttt
    21421 cacctaaccg ttcaggtcag tgcggtgata agtgcatcgt ctggcccgac cgtctcccct
    21481 tgattttcca aaatatccaa ataatccatg taattggtga cgattacagg tgtttctaaa
    21541 ctgtatcctt ccttttcgat tttctttttg tcgaagcgga gcaacagatc gccttgtttc
    21601 actctttatc cttgctgcac taagctttca aaatatttcc cttccaactg cacagtatcc
    21661 aatccgacat gaatcagcaa ttccagccct gtttcagaaa ccattccaat tgcctgtttc
    21721 gtcgggaaca gggtcatgac tgttccgtca aaaggggcac gtacttctcc tttttcaggg
    21781 tagatcaaga ttcctcgacc tagtgttcct tgtgcaaaag ctgcatcctt tgctgcattt
    21841 agtggagcga tttgtccttg tatcggtgtt tttacaattt ctttacctga ttttttctgt
    21901 atatcatctg tttcacctgc ttcatccttc caaaagaaaa aagtcagcgt aaaggatacc
    21961 accatcgcaa tggcgatccc aatcaataca ttaatcacat ctgttaaatc accatctggt
    22021 ctaatatagt tgggaattgt aaagatgcct agtccacctt gattccaaga agttaaattc
    22081 attaacccac tatacgcgcc ccctaacgca ccaccgacac aggtaaaaat aaatattttt
    22141 ttacgtggta atgtaaaccc ataaatagct ggctctgtaa tccccacaat accagaaaca
    22201 atggctggta ttgcaattgc tttgtctttt ggatttctaa gcttaaagta gagtgcggta
    22261 agtgctgcag tttgtccaaa agacactact gcaacaggtg tcataatatt ttgccaacca
    22321 ttcacagaaa attgcatcaa tcccattggt acgattgccc aatggagacc aaacatcacc
    22381 agaatttgcc agaagaagcc tacgattgct ccataaataa ctggcgaaag tgctaaaagt
    22441 gtttcaaatc cagccattaa aatatcagtt gcgaatgtga atactggtcc aacgagtaaa
    22501 aaacctaaag gcattgtaat aagaacggtg aagaatggaa cgaagaaatt cgcgatcatc
    22561 tcaggtacta actttttaat tggtttctga atttggctag ctaacaacac aataaagata
    22621 atgggaatga cagaagaagc gtagttacgc gcaatccagg gaatgccgaa tactgtttga
    22681 taaataggtg cttcaaaaat agtgccagaa aatatggtcg ttagtggtgc ttctgctgca
    22741 ccactcaaag cttccagcga caagctagga ttcattaatg ctgcgccaag caacatccct
    22801 acgaattcat ttactttaaa tttacgggca gctgttaaac cgataataac aggcataaat
    22861 tggaatacag aatcaccgat attatcaaac ataacaaatg tgccagaacc gcggtcaaac
    22921 gcgcctaaga agacaaggaa tgctgtcagt ccacgaatca tcccgccagc agctaggata
    22981 gcaaggaatg gctggaaaca gcccgatatt ccatcaataa gcatatcaaa cggtctcatt
    23041 ttttctgttg aaacagcctc atcaaaagta aggtttcctg cttttataac ctcttcaaac
    23101 acgactggca catgattccc aatgacaact tgatactgcc ctcccgctcg cattaccgtt
    23161 acaataccgt ccatattttt caagatctca tcatttgctt tttcttcgtt ttttaatttg
    23221 aaacgaagcc ttgtgataca atgtgtcaaa ccattgatat tttccttccc gccgacattt
    23281 tctacgattt ttctcgctaa ttcttggtat tttcccattt ctattccctc caatttatta
    23341 gtggaaggtt tagccaactg aatgtcacta cccttatttc atacgttctt gttaatttat
    23401 cctccttttt ttatttacaa cgattctcat gcactaaacg cgcagtatgg atcgttaaat
    23461 acattttttc gtccgtggac atctggtagt tgaacgtctc ggctaaaaag tctgctattt
    23521 tctcaacgca accaaacgta tccttatatt ttttttgaat aacctgaaac aattcctcag
    23581 aaacttctcc cacttctgat ttttctccat ctaggacgcg tgtcagaaaa aaacgtaagt
    23641 gtgtgacgaa tcgaaaaaaa tggactgaat cttcatcaaa tgtaatctta tagtaatagc
    23701 ggacgatatt tagaatatcc tgtattgtta tagtaaacag gtaggtattg tcttgctggc
    23761 tttgaatgcc ttgtgcattg actaaatgca atgcgataaa tccggcttca tcttctgata
    23821 acttgatgtg gtatttatcc gagattttct tcactgtttt tttcccgatg gcatactctt
    23881 taggaaatag ttgtttgata tcatacagca atacatttgg tacaaatatt cccattttgt
    23941 gacgttcgat agccgtatac aaatgatccg tcaatgcaat atacacagaa tcattcaact
    24001 ggacttgtag agcctctttt gtatattcaa tgatttcaca agaaatttct aagtactcaa
    24061 gaggaatcgc ttgtgccaat tcttggaact gttgtgaaag cccgtcattt gtcaactgaa
    24121 attttttgtc gatgcatgag tcaaaaatcg ggtcacccac tttctttttg aatgccaaac
    24181 cttttcccat cacgatcatt tcctgatttc tttcatccct ggttattacg acgttgttat
    24241 tcagtacttt ctcaatcagc atgttttgct cctttccatg atgtataggg ctgtatccaa
    24301 aacaaaaaga aaaacccaaa aagccgtcca tgaaaccatg ggagactcat caggtttagc
    24361 ttgcatcagg caatcactat ccaagttgta tcgttttctt tatattatcg aaagcaaatg
    24421 cttttgtcaa cgctttctta gtggctatta ggcacttctg actagctgtc gaatcttttt
    24481 ttaaatctgg tgactacgac actaactctc acagatgaaa aatatgagaa gctatgtttt
    24541 attttcactc tagtgcttag gtcaaaattc tactgtcaca acatctttta tataccactg
    24601 ctttgcagtt tgtacatatc tgcatacaaa ccattttgcg ctagaagtgt ttcatgattc
    24661 ccgcgttcta ctatatgacc tttgtctaaa actaaaatca attctgcatc tcgaatcgtt
    24721 gacagccgat gggcaatcgc aatcgttgtc cgtccttgac gcatttttgc caatccttct
    24781 tggatcaatc cttctgtttc agtatcgata tttgctgttg cttcatccaa tactagaatt
    24841 ttaggatcag tcacgattgt tcgcgcaaaa gtgatcagct gtcgttgtcc actagaatag
    24901 ctggcaccat gttcgatcac ttttgagtga tatccttttg gtaattcatc gatgaatttg
    24961 tttgcttgaa caaactcagc cgcatggcgt atctgttcgt ctgtgatagc tggattcatc
    25021 atccgaatat ttcctgcgat atccccataa aacaagaaag catcttgcaa gaccagcccc
    25081 attttcttac gtaactcttc cattggatat tctcgaatgt catgatcatc aatcaatatc
    25141 tgaccttcat aaaattcgta aaaccgcatc aatacgttga tgatcgagct ttttccgctt
    25201 ccggtgtgtc ccactaatgc aacggtctct cccggattag caacaaaaga gatattgttt
    25261 aatacattgt gctctccatc ataagaaaag cttacattgc gaaactcaat cttacctgaa
    25321 gtaatcattt gatctgctcc agggttttgt tgtggtgcat attcttccgt atccataatc
    25381 ttcaagatac gactaccagc aaccatccca tctgtgaaga tgctcaaaaa gtccatcatt
    25441 tgagacattg gattaaaaaa tccctggaca taggtcgtaa atgcatagat cagtccaact
    25501 tctacgggag aatgcaacgc atcgatccca aacaaagtca acaccaacgc aatcgccaat
    25561 gcatatagaa aattgatgat cggacttaac agaatcgaat tcgttttgat catcgcataa
    25621 cgcgtgtcta aatactcatt gttcacttgt tcaaattctt tagctaaacg agcttcttgg
    25681 cgaaactgct ggataatacg catgccggaa atcgattcat tcaatttcgt gttcagctga
    25741 cttaattttt cccgcatatt tcggtaaata cgagaactga atttctggta ataccagatg
    25801 acgattccta aaatcggcaa aaacaccaaa gtccacaagg caatttctcc attgatttga
    25861 aacatggcaa ggaatgaaga aacgactgca aagatcccag tcaataccat caaaaagacg
    25921 taccaaaatt caaatagcgt ttctgtgtca ttcgtcactc gagaaacggt tgatcctgcc
    25981 ggtgtctgat caaaataacg catacctagt gtatgcagct tttcaaataa ttttacccta
    26041 atatactgat aggtcttcaa tgaggccatg gaatacaaat accattggaa aaaccaaatg
    26101 atacttttta ctatgactcc aaataagtac ataccagcaa aaaacaaaat aacttgtgtc
    26161 gtagccgact gtttagccag atgatcatcc ataaaagttt ggatgatcct tggcaatagg
    26221 acattgatca cagacaaagc aaacgcaaat aagatagcaa cgatgaacgt tcttctaaaa
    26281 ggtttggcga acttcatcag tcgtttgatg atctttttct gttctttaag tggaatcgat
    26341 tttgtccatt ccgattgata attttcatcc atcaggcctc gcctccttct atcttcgctt
    26401 ctaattgctg tttctcccac atttgttcat accagccgcc tagtgcaagc agctcatgat
    26461 gggttcctcg ttcaatgatt ttcccttgat cgacaacaat gatttctttt gcatgcatca
    26521 cgctacttaa acggtgagct gcaatgatag ttgttttctc ttttcttatt tcttttaaat
    26581 tatgcaagat agcttcttct gttttggcat ctactgcaga gagtgcatca tctaatatta
    26641 ataattcagg atttttgatc aatgctcgag caatcgaaat cctttgtttc tgtccgccag
    26701 ataaagagac tccacgttct ccaactaagg tatcataacc ttttggtaat tccttgattt
    26761 cattgttgat aaatgctaaa gaagccgcat gctctacttc ttcttctgaa agttttggat
    26821 tagaaaaacg gatattatct cgaatggtca tcgaaaacaa gaagtgatcc tgaggcacat
    26881 agccgattga tcctaataaa gcattcaatg aatattcttt gatcgaacgt ccgccaaatt
    26941 ggattttccc ttgataatca tcgtattcac gcatcagtaa tttgatgatc gtcgtttttc
    27001 ccgcaccagt tttcccaaca atacctaatg tttctccttc ttggatcgca aacttgattt
    27061 gttctaatgc tggtgattct tctttcggat aattaaaaga ggaaatagct acattcagct
    27121 cgccttttgc cggggtctgc actgcaccca gttgttccac gatatgcgtc ttttcatgca
    27181 aaagttcatt gacgcgatca taagaagcat ttcctctttc caatacgttg aacaatcgac
    27241 caatcgcaaa catcggccaa acaagcatac cgatataact aataaatgag accatctgcc
    27301 cgatcgagat cgtgccttcc aagacatact ttccacctaa aataattgtt gcaacataag
    27361 acagaccgat aatcaaggta atgaatggat caaataagga gtctaaaaaa ttgactcgtt
    27421 tgtttttaat aattgcatct tcaatctttt cttcaaaatc cgctagatct tctttttctt
    27481 gtccaaatgt tttaatgact ttgatcccag tgatactttc ttgtgtctta tcattgatac
    27541 tagaaaaagc ttcttgcgaa tcacgaaaag catcatggag ttttgacccc agcactcgtg
    27601 aagtgacggc cagcaaaggc agaggaatca gcgcaatcag tgttaagcgc caatcaataa
    27661 acaaaatcat cgcaatgatc gttgttccgc ccgtaatcac tgaatctgca aaggtcaaga
    27721 taccagctcc tgctacattc tggatcgcat tcaaatcatt cgttgcgtga gccatcaaat
    27781 ccccagtccg atatttttgg taaaagagat tgtccatttc tgtaaaatgt ttgaataact
    27841 gctgccgaag ttctttctcc aatcgagctg cacttcccca aatattcgtc cgccagatat
    27901 aacgaaaaag atattgtaaa aaagcagcga taatcaatag gaagatccac ccgaaaatga
    27961 tctttattga taaattttta tcggcgatct catcaatgat gatcccaatc acttttggag
    28021 gaactaactg taccaaggcc actgcgaata gcgagaaaac cccaattaaa taatgtttct
    28081 tttcctgacg aaaaaaccag cctaattttc taaaaatcga cattttactc ctcctttgtc
    28141 tttatcaaga gcctaggatt gtctgtgctt gtcttttttg ctgaataatc tgaatagtct
    28201 aacaaattac gaaataaaaa acggactgaa aaaatcagcc cgtaaaaagt cgttgggtca
    28261 gaacctattt tttcccttgg ttcttcattt gctgcatcat ctggcgaatt ttcttctcag
    28321 atggtttttg ccccattgac atcatcattg atcgcaacat gtcctcatta acgggaggat
    28381 ttttttctaa atagtctttc atatatttac gcgctaggaa aaagccacct actgcgccga
    28441 ttagcaaagc aataatagca ataagtacga caatacctgt tgataacata taaatctctc
    28501 ctttctattt ccgaattgct ctcaatgact tattttacta aagtttattg aaaaaagaaa
    28561 ggcgtattct ttagtttcat gcaaaaaata gtggtttttt tagaggagaa caaaatttct
    28621 ttcgtcatct agcaagacgc gccatgaaaa aaaattaggc gtaccgaatc tttttcttta
    28681 attttttctt tttgaggtgt agaatgtaat cgtagataaa taggaaaggt ggttttttct
    28741 tgaataatca atccaataaa caatttcata agcatcgact tattgaacat acaaatggtt
    28801 tatctttagc tgaagtaaat ggaacagttg atgtaccaaa ggggaaaagt ttttttaaaa
    28861 cacttcttgc ttactctgga cctggtgcgt tagtagcagt tgggtatatg gacccgggta
    28921 actggtctac ttcgatcacc ggaggacaaa atttccaata tttattgatg tcagttattt
    28981 tgatgtctag tctgattgct atgcttttac agtatatggc agcaaaatta ggaatcgtga
    29041 gccaaatgga tttggcacag gcaatccgag cacgcacaag taagacatta ggtgtcattt
    29101 tatggatctt gactgaatta gccattatgg caacagatat tgcagaagtc attggtgcag
    29161 caatcgccct ctacttactt tttgatattc cattggtgat tgccgtcttt attactgtat
    29221 tagatgtttt cgttctctta ctactgacaa aagtcgggtt tcgtaagatc gaagcactgg
    29281 ttgtctgctt aattttcgtc attttattcg tttttgtcta tcaagttggc ttgtctaacc
    29341 cagattggca cagtttattt gctggcttta tccctaacag tcatacattc tcagactcac
    29401 cggtcatcgg cggcgaaact ccgctgactg gagctttagg gattattggt gcaacagtca
    29461 tgccgcataa cttatatctt cattcagcag tttcacaaac tcggaagatc gatcaccaaa
    29521 acgaagaaga tgtagcacaa gctgtacgtt tttctacttg ggattctaat atccagttgt
    29581 caatggcttt tctagtcaat gcactgctat tgatcatggg tgtcgctgtc ttcaaaaccg
    29641 gttctgtcca agatccttcc ttttttggtc tgtatgaagc tttatctgat ccatcagcta
    29701 tgagcaatgg tattttgatt gctgtagcaa aatctggtat cttgtccact ttatttgctg
    29761 tagcacttct tgcttctggc cagaactcta ctattactgg tacgttgacc ggtcaagtca
    29821 tcatggaagg attcatccat atgcgtatcc cgatttggct aagaagactg gtcacacgat
    29881 tgctttctgt gattcctgta ttgatttgtg ttttgtttac aagtacaaaa ggaacgatcg
    29941 aagaacatgc cgctttgaat aacttaatga atgaatccca ggtgttcttg gcttttgcgt
    30001 tgccgttttc gatgattcct ttactgatga tgaccaacag ccaagcggag atgggtagcc
    30061 gatttaaaaa ttcattcatc gtaaaacttc ttggctggtt ctctgtgatt agtctaactt
    30121 acttgaactt acgaggatta ccaggacaga tcgaaagttt cttcggcgag caagccactt
    30181 ccgctcaact agcattggct gatcacatcg cttatgtaat cattgttctt gtccttctcc
    30241 tgttggcatg gacgattggt gatttatata aaggaaacaa aaaatatgaa cagcttttgc
    30301 aggaaacggt agaagaaatc gaataaaaac tgatttttcc acacaaagac ccttgatagt
    30361 taggttaaaa atctagctac caagggtctt tttatttaga agataaagct tttttcagat
    30421 ttaatttact gaaaactagc atgcttgtta ctacgatgaa ttgcgttaat ggcacgcctg
    30481 caccagtgta gacacttaaa aaatagtcct ttttttctgg cgcataagaa caaaaccatg
    30541 agacaccgaa aagaaaaacc aattgtgtca gtattaatcc ggtgaccaca atcaaagagg
    30601 aactataata ggcacgatca gcttttgcaa atacggttcc tagtatcata agtaatatcc
    30661 ctgctattga agcaatcagc acgggaatag ccagaacttg gtaaattttt atgatgacat
    30721 tcgcgacttt tgctgatttt gttaattgga aattaggtgt tctcggttca ttactatcga
    30781 tccaattctc cattagcgga aggtttaata attcttgagc ctgtttgact tgttcttgcg
    30841 ttccgaaact ataatttcct ccttgatgga aatctttata tcgccatgca taagcgtatc
    30901 ctgattgaag gaaatcgaat acgattggca gatcactcat cgtctttcca tctcctgatc
    30961 ccatcagata gaactctttt ttctttttga ttgttccctt gtgataggct tcttctaatt
    31021 cttggttgac ttttttccaa aaatcattgg tttcttttgc attgttttgg taataaccag
    31081 attcgcttac cacatctcgc aatgcccaaa agatgatatc tccgggtaat tcttttactt
    31141 ttccaccttg agtccacgcg tgagtcgtat ataattcgtc aatacgggta gaaacagtat
    31201 ggaatgtagg agatgcggct aatgctttat ttagtgcagc atgtgagacc catatctcac
    31261 tgttttcttt attcaagttc gtttcatcat ctattcggat cagttgtttc atcaaacgtc
    31321 caaattctcc actggaacgg tcattgatgg ttgcaatacc gtatgttgtc tcatttttat
    31381 attcgatcca ttgatagacg atggaaaagc caactatagg aagtaaaaac aatgaaagtt
    31441 tcagccattc ccgtttcgag agaagaaaga gtcgtttttt taacttcttt acttctttga
    31501 aagaaaagtc ttgccctagc aagatctgga cgaaactgat acccaaagct actacagtaa
    31561 aaggaagaag ccagatagaa tcttcgcgaa tgaaccaaaa gaaaggaaaa gcgacagaaa
    31621 gccctaaaaa ccatggcagc aattttctgc tagaaccgta tttgcggtaa taaatagcta
    31681 ataaacaagc gataatgatc tcaacagccg gaatcacgag ggcattacga tatgttcgag
    31741 tggaatattc atgtgtaaaa ctaatcggag aaaacaaaaa ataaaaatag atacttaaaa
    31801 gaagatactt gttcttcaca atcggtttaa acgcgagtgc aaccaatata ctggcaaaaa
    31861 tactgaacat gccgaacaaa actgcataag aaagatgcag tttatgtgca agtactaaaa
    31921 ataagctgta ggagattcct ttggaaagtg ttgtagtcgt atactcccct agccatttac
    31981 cgtccaaaat attttgagca tagcgaatca gcagctggtc atcataggtt gccgacatgt
    32041 ctgttccata gattgcttta ctatataaat acaaacgaat aattgtaaat aatagggcta
    32101 tgccaagaat aaagtatgta tgtttctttt ttagttggtt gatcaagggt tttaaagtgt
    32161 tcagcatctt ttcgtccttc caatcacgtt ttgccacttg cttgtgaaac ttttcgtttt
    32221 tttacttttc taccagtata ccttgtttgt ctaaatttaa aaagaggggg gagaagatgc
    32281 atgatgaaaa agactgcaaa aagtactcct ttgtttctta aacaaaaaag gacaaggcaa
    32341 tgtgcctggt ccttttttgc tgctgtcaga tagaaaatct tagagtatca cttcttctgc
    32401 taataactca ggaaacactt ctggatcgaa tgtttgcgct ttgaacatgg cgatttcttt
    32461 tccatatggt gcgtaagctt tctttttatc tttgcctaca tagggagttt ccaagatttt
    32521 cgaaatattg tcaaacattg gatcatggac gatcttattc agcgtgtcga atccgattgt
    32581 tccaaaacca atattggcgt gccggtcttt atgcgaacct tgcggatttt tagaatcatt
    32641 gatatggagt actttaagcc gatctaaccc gatgatctta tcaaactctt ccattacacc
    32701 tgaaaagtct tctttaatat tataaccggc atcattcgta tgacaggtgt caaaagtaac
    32761 ggataatttt tcattcaatt ttactccatc gatgatttgc gctaattcct caaatgttcg
    32821 accgatttcc gtacctttac ctgccattgt ctccaaagcg atttgtgccg tctgttcttt
    32881 tgtcaaaact tcattcaagc cttcaataat tctagcaatt ccggcttctg ttcctgcccc
    32941 tacatgagca cctgggtgaa gtgtaatttg agtagcgcct aaagcttccg cacgaacaat
    33001 ctcttctctc agaaattgga cggcaaaagg aaacatttct ggtttgatcg tattccccaa
    33061 attaatgata taaggggcat gaacgacgat atttgttaga cctttttctg ccatgtacgc
    33121 ttttccggca tcaatattca tctcttcgat ggcttttctt ctagtgtttt gaggggcgcc
    33181 tgtatagatc ataaaagtcg aagatccata actggctgct tcttctgctg ctcctaacag
    33241 cattttcttt ccgctcatgc tgacgtgtga gcctaataac atggatggat tcctccttga
    33301 aaggcaattg ttactaacaa ttcctatata ttggtaagat gatatacaaa gaaaatgatt
    33361 gatttttctt tgccctgttc tccatagtaa ctgaaatttt ctctaaagaa aagcttaaga
    33421 actcttttat aataaagatc attttctcac gttttaactc ttttaaaaaa gaaattcctg
    33481 ccaaaaacag ccttgtctgt ttttttgttc tattatttgc tataattagc gaaataaaac
    33541 cattagttga ggtgaccctt ttgccacgca aaaatactcg aaaaaaacag aatagaagaa
    33601 aaaaagaaaa atggtttgtt cctaagatta tttttagagt ttttcaatca ttgactgttt
    33661 ttattactgt tcttcttttg atgttcgcag ctctaggtat tggaataggc gctggctatt
    33721 ttgcctatct cgtcgaagac acacaattgc cgactaaaaa agcactgcag acagaactag
    33781 gaaacattac agaaacgtca aagattgttt atgcagataa tacagagata tcaaaaatcc
    33841 agacagatct gatgcgcacg actatttcat ctgataagat ttcaccttta ttgaaaactg
    33901 caattatcag cacagaggat gaatacttcg ataaacacca aggttacgtt cctaaagctg
    33961 ttttacgagc attagtatct gaagctactg gtattggctc ttctggtgga tcaactttga
    34021 cacagcaatt agtcaaacag cagatcctta cagatgaaac aacattcaaa cgtaaagcaa
    34081 acgagatcat tctttctgca caagtagaaa aatacttttc aaaagatgag atcattgcta
    34141 cgtatttgaa tgtctcacca tttggtcgta ataataaggg acaaaacatt gctggagtcc
    34201 aggaagctgc aaaaggaatc tttggtgtag atgcaaaaga tgtgacgctg ccgcaagctg
    34261 cttatatcgc tggattaccc caaagcccga tcacgtatag tccatacacg aacacaggcg
    34321 cgctaaaaga agatctttct gccggccttg ccagaaaaga ttttgtttta ttcagtatgt
    34381 atcgtgaagg ccaaattacg aaagaacaat acgaagaagc aaaagcctat gaccttacaa
    34441 aagactttct accacaacaa atcgcagaac agactgacag agaattcttg tattatactg
    34501 tgatgaatga agcaacaaga atcattgcac aacaattggc tgaaaaagac aacgctgata
    34561 tgtctgattc atcggtcagt gacgcttatt atcaaaaggc acagcaaacg atccaaaata
    34621 aaggatatac aatccattcg acaatcgata aagatgtcta tgctgcattg caaaatggcg
    34681 ttgaaaatta tggatacatg ttagatgatg gtgctggcag tcaagtagaa accggaaatg
    34741 tattgatgga taacagaact ggccgcatct acggattcgt tggcggacgg aattacagtc
    34801 agaatcaaaa taaccatgcc ttcgatacag aacgtcaagc tggctcatca atcaaaccag
    34861 ttcttgttta tggacctgca atcgacatgg ggcttatcgg atcagaatcc cgtgtatcag
    34921 attatgcaac aacttggcaa gaaggagaaa atgctggcga gaaaatcgtc aatgctacta
    34981 acgaaggatc gaacaccttc caaaccgtcc gtgagtcact agaatggtcc aacaatatcc
    35041 cggcttatca cttatatcaa gatgtgttga attccggtgg atcgaaacaa tatgcttacg
    35101 agcactactt ggcaaagatg aactacccgg caaatgacaa ttggggcgtt gaatctgctc
    35161 ctttaggtac agtagatgtc acgacgcttc aacagaccaa cggatttcag gctttagcaa
    35221 acggcggcgt ctatgaagaa ggctatatca tcgattccat cacagataac gcaggaaatg
    35281 taatttataa acacgaaagc aactctgtcc ggatctattc ggaagcaacg gcatcgatca
    35341 tgaatgacat gatgcgtagt gtgatcaatg caaaaatcac tacaccattc aaagatgcca
    35401 tctcaagctt aaacggaaat ctaggaaaag cagattgggt cggaaaaact ggttctacca
    35461 atgaataccg agattcatgg ctaattgtct ctactccgtc tattaccatc agtagctggg
    35521 cagggcacga tgacaatact ggaatggaca gcaaagcaag gatacgttcg gctaattatc
    35581 ttgccaatct gatcaatcaa gcttaccaag caaaacctga catttttggc actgatgaga
    35641 aattcgaatt atcatctgat gtgatgaaga aaaaagtatc tggatttact ggtcaaacac
    35701 ctggaaaagt tactgtcaat aaaaaatcca tccaaacacc aggaaaaaca gttgaatcgc
    35761 tatgggcgaa aaatggaccg aagaaaagcg cctttaaatt tggcgttggc ggtaccgatg
    35821 aaaactatac agattactgg aacaccgctt cagcttatgc aagagcgaat caagcaacta
    35881 aagaatcaga tgagaaagat gattgattta atagaataca cagtaataag ggattgtgac
    35941 aatagtattg tcacagtccc ttattttttt gatggtggtg aacaaaatgc agcatctata
    36001 cacggattgt tttatatacg ataaatgtaa tttcaaccac tttctctctt ttttaagaca
    36061 caaaaaaagg attataacaa aattttgtca taaccccttt tcccaaataa acggtgttct
    36121 cctgccaact ccgaatcacg agtcacaata ttcatcaaac atgagatgct gttttttgaa
    36181 attgctcctt gtaacgcaga gttaaacggc aggcacacaa cctctgaatg gttgtccttt
    36241 tattattttg tagatccttt ttcttcgatt ccataaggca agataatcgt tttttcttcg
    36301 acttcttctt tattcatcat tttcgtcaat aggcgcatag ataccgcccc taaatcgtat
    36361 aatggttgtg tgatacttgt taaacgagga cgagctactt ctgtcagtag tgaattgtta
    36421 cttgtgatga tttcgaattc ttccggtact ttcacgccac ggtctaataa accatccaaa
    36481 ataccgatgg ctaattcatc atctgaaaca tagacagctg ttgcaccgct attatggatc
    36541 cgatcgacta gagatagacc atctttgaag ttgtattgtg cttcaaagat caatccttcg
    36601 ctatatggta ggttattttc gcttaatgct tctttatagc ctttcagacg attttgtcca
    36661 ttgatgggat cgatcaacgc accactgaca aaagcgattt ttttattgcc gctttttgcc
    36721 aatttgttta ctgcatcttt tgttgcggat acataatcaa tgtttacgct acctacttgt
    36781 tcatctggat caatagagcc agctaataca acaggagttt ttgaacgtga aaattctcca
    36841 cggatttcgt ctgtgatatg gtgacccatg aagatcacgc cgtccacttg cttagccagt
    36901 aagttattca atacattgac ttctttttgg tcgttgccat ctgaatttgc taagataata
    36961 ttgtatttgt acattgttgc cacatcatcg ataccacgtg ctaaagaagc aaagaacata
    37021 ttgctgacat cagggatgat tacaccaact gttgttgttt tcttacttgc caagccacgc
    37081 gcaactgcgt ttggacgata atccaagcga tcgataactt ccaagacttt ttttcttgtt
    37141 gctggtttaa cgtttggatt gccatttacg acacgtgaaa cagtagccat cgaaacattt
    37201 gcctctctcg ctacatcata gatagtgatt gtttgctttt ccatttgtgt tctcctattc
    37261 atattttata tgttttctga aaatacgtct tacatttcta aaatagaata tcaaataatc
    37321 tcaagaattg caaccgtttt atctctgttt tcatgaaaat ttttactttt ttcagttttt
    37381 agaacgttgg tcttttaata aatcagtggt acaattattg aatacaaaat taatttagaa
    37441 agtaggttgc caaatgaata acgtaaaaat caatgaacta cgtcaatgga tggcacaaca
    37501 ccaagtcgaa ttggcttata tcagcgaccc tggccacatc gcttactttt ccggatacga
    37561 aagtgatcct catgagcgtg tactagctct ttttatccct cttgaacaag atcctttcct
    37621 tttcactcct gctttggaag ttgaagatgc tcaaaacagt agttgggcgt atgatgtccg
    37681 tggctattta gacagcgaaa acccttggga gatcatcgga aaagaacttc gtgctcgcta
    37741 tggtcagcct ggcaatgtag ggattgaaaa gaacgccttg actgtcgagc gcttcgatgc
    37801 tatctctgat atcttgacgg gaacggattc ttacactgat ctaacttcca tcatccaacg
    37861 tctacaactg atcaaaactc tagaggaaaa agacaaactg atcgaagctg gtaaatgggc
    37921 cgatgttgca tttgagattg gctttaaagc agtgaaagaa ggcgtgactg aacaagcgat
    37981 cattgcagaa attgaatacc aattgaaaaa acaaggtgtt tcacaaatgt ctttcgacac
    38041 attagtcctt gcaggagcaa atgctgcaag ccctcatggt acaccaggaa atacgaagat
    38101 ttcaccgaat gaacttgtgt tattcgatct tggcgttgtt tggaatggct attgcagtga
    38161 tgctactcgt acaattgctt atcaaaaacc aaccgacttc caagaaaaaa tctacaacat
    38221 cactcttgaa gcacagcttg ccgcacaaga agccgtccgt ccaggtgtga cagcaggtga
    38281 acttgatcaa atcgcacgaa acgtgatcaa cagctatggc tacggcgaat atttcaatca
    38341 ccgtttaggt cacgggatcg gaacaaccgt ccacgaattc ccttcccttg tagaaggaaa
    38401 tgacttagta atcgaagaag ggatgtgctt ctctcttgaa ccaggaatct atattcctga
    38461 aaaagtaggg gtacgtatcg aagactgtgt atatgtgaca agcgatggct gtgtgccatt
    38521 cacgactaca ccaaaagaat tacttgtttt agaaaaataa gagtaaaaaa ggtgagagct
    38581 agtccgctcc caccttttt
//
LOCUS       DAPCMU010000011        37208 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPCMU010000011 DAPCMU010000000
VERSION     DAPCMU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.011"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            53..802
                     /locus_tag="RD744_000463"
     CDS             53..802
                     /locus_tag="RD744_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0556494.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1226..1396)
                     /locus_tag="RD744_000464"
     CDS             complement(1226..1396)
                     /locus_tag="RD744_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0556495.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1590..1769)
                     /locus_tag="RD744_000465"
     CDS             complement(1590..1769)
                     /locus_tag="RD744_000465"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556496.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1779..1955)
                     /locus_tag="RD744_000466"
     CDS             complement(1779..1955)
                     /locus_tag="RD744_000466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556497.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2156..3268
                     /locus_tag="RD744_000467"
     CDS             2156..3268
                     /locus_tag="RD744_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0556498.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3346..3639)
                     /locus_tag="RD744_000468"
     CDS             complement(3346..3639)
                     /locus_tag="RD744_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDU0556499.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3807..4121
                     /locus_tag="RD744_000469"
     CDS             3807..4121
                     /locus_tag="RD744_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDU0556500.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4142..6121
                     /locus_tag="RD744_000470"
     CDS             4142..6121
                     /locus_tag="RD744_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0556501.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6134..6460
                     /locus_tag="RD744_000471"
     CDS             6134..6460
                     /locus_tag="RD744_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDU0556502.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6476..6994
                     /locus_tag="RD744_000472"
     CDS             6476..6994
                     /locus_tag="RD744_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556503.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6994..8346
                     /gene="celB"
                     /locus_tag="RD744_000473"
     CDS             6994..8346
                     /gene="celB"
                     /locus_tag="RD744_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDU0556504.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8430..9935)
                     /locus_tag="RD744_000474"
     CDS             complement(8430..9935)
                     /locus_tag="RD744_000474"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDU0556505.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10027..10617)
                     /locus_tag="RD744_000475"
     CDS             complement(10027..10617)
                     /locus_tag="RD744_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0556506.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10796..12442
                     /locus_tag="RD744_000476"
     CDS             10796..12442
                     /locus_tag="RD744_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0556507.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12491..13210
                     /locus_tag="RD744_000477"
     CDS             12491..13210
                     /locus_tag="RD744_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDU0556508.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13689..14636
                     /locus_tag="RD744_000478"
     CDS             13689..14636
                     /locus_tag="RD744_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0556509.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14648..15568
                     /locus_tag="RD744_000479"
     CDS             14648..15568
                     /locus_tag="RD744_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0556510.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15609..17066
                     /locus_tag="RD744_000480"
     CDS             15609..17066
                     /locus_tag="RD744_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0556511.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17411..18025
                     /locus_tag="RD744_000481"
     CDS             17411..18025
                     /locus_tag="RD744_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0556512.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18018..19742
                     /locus_tag="RD744_000482"
     CDS             18018..19742
                     /locus_tag="RD744_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0556513.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19739..21190
                     /locus_tag="RD744_000483"
     CDS             19739..21190
                     /locus_tag="RD744_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0556514.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21663..23816)
                     /locus_tag="RD744_000484"
     CDS             complement(21663..23816)
                     /locus_tag="RD744_000484"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDU0556515.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23818..24864)
                     /locus_tag="RD744_000485"
     CDS             complement(23818..24864)
                     /locus_tag="RD744_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0556516.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25014..26303
                     /locus_tag="RD744_000486"
     CDS             25014..26303
                     /locus_tag="RD744_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDU0556517.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26316..29018
                     /locus_tag="RD744_000487"
     CDS             26316..29018
                     /locus_tag="RD744_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDU0556518.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29003..29860
                     /locus_tag="RD744_000488"
     CDS             29003..29860
                     /locus_tag="RD744_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0556519.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29931..30830)
                     /locus_tag="RD744_000489"
     CDS             complement(29931..30830)
                     /locus_tag="RD744_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDU0556520.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            30999..33170
                     /locus_tag="RD744_000490"
     CDS             30999..33170
                     /locus_tag="RD744_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDU0556521.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33238..33579
                     /locus_tag="RD744_000491"
     CDS             33238..33579
                     /locus_tag="RD744_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDU0556522.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33855..35609)
                     /locus_tag="RD744_000492"
     CDS             complement(33855..35609)
                     /locus_tag="RD744_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556523.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35620..36456)
                     /locus_tag="RD744_000493"
     CDS             complement(35620..36456)
                     /locus_tag="RD744_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDU0556524.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36458..37141)
                     /locus_tag="RD744_000494"
     CDS             complement(36458..37141)
                     /locus_tag="RD744_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDU0556525.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12004 a         6542 c         7629 g        11033 t
ORIGIN      
        1 tgaattagat gcgacaccga tcgagtttga gatcgaaaga agtcagacag cggtggtaga
       61 attgacgaaa gaaaaccgac ttacaccagg cggtgtcgtg ttgacaaaga tcgatgacca
      121 aagcggggaa gtcctgcaag gagctgtttt tgagttacaa gatgccaatg gaaccgttct
      181 gcagtcaggt ctaaccacag gagcagatgg aaaactggcg attgacggct tagcaccagg
      241 agtgtatcag ctggtagaaa cccaagcgcc aacggggtat gaattggatg cgacaccaat
      301 cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag aaaatcgact
      361 tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggagaag tcctgcaagg
      421 agctgttttt gagttacaag atgccaatgg aaccgttctg cagtcaggtc taaccacagg
      481 agcagatgga aaactggcga tagatggctt agcaccagga gcatatcagc tggtagaaac
      541 tcaagcgcca acggggtatg aattggatgc gacaccagta actttcaaga tcgaaaaaga
      601 acaagaagtg gcgatctttt taacaaaaga gaatagaaaa gttgctgata gctcagatat
      661 aagaggaaac aatccaacaa gcagtggaaa taagcatctt ccaaaaactg gagaaaccat
      721 cattagccag tttatattgt caatattggg tgtgctactt gttttcatca gtataaaatt
      781 tagaaagaaa cgaaatatat gagctaaaat aaaaaattga ataagcatag tttttaaaat
      841 caactagtac ccatcaattg aatggaaact accgatttta gaaggggtat acaaacaaat
      901 attagtatcg gagtagagaa ggttgaaccc aaccagtaaa taagtaaaga caattttgtg
      961 ctgcgttgag aagtgaaaaa taaacctttg tagtaatact agttaataaa aaatatacaa
     1021 aaacactgcg aaaatcattc ttgagtttcg cagtgttttt tgcctaatga ctcaagttta
     1081 ttgggtaata ctttgatttt ctttctacaa ataatcagcg atcagttgta attttttctc
     1141 tagttttaat ggctctttgt caataaggag aaaaacaagt cgggtctttt gtctcggaaa
     1201 gcttcaacca actcgtgaat aaaggttacg cctccttgaa aggcgtggaa aaaccaagta
     1261 atcgatcaat caccaatttc gcatttggta gaacatgttt ggtgagctga aagtaggtgg
     1321 cgttcatgtc tgtaactaag aattctattt ctttaggatt agtgcagcta aggaaatagt
     1381 ttgttaatcg aggaattttt tgtgtaggta aattttctcg attaattttt ctgtctcgcc
     1441 atccgcggaa ataaaactca gtttatcctc aacggaaacg tgagaacgaa attcacaacc
     1501 ctcaacactc aagacagaat tcttttggaa gactttggta aatagcttct caactctttc
     1561 aatgtgcgaa taacggttgt caaagatacc taacaacttt tcgaaataaa aaataacgac
     1621 actttttcag tcagtaaaga agtgatttta tgtctgacat gatttgaaat cgagtgcctt
     1681 ggacggataa aataactttg agcgttccaa tggcttcgac agtttttaca ggtatagcgc
     1741 tgctttttta ggcgtataac cattggcata tgattatatt attcaaagtg gacaatggtt
     1801 tccttcttct tgtttttcac caccattaat ttcctatttc catctaccac agcagaacca
     1861 caatttctac agacacatgg agcagggaag agaactgcag caacgatcaa tgtttttttc
     1921 ttctgtaagg tttcgtaaga gacctctgta atcatcaaat ttttgtctat tgatctcaga
     1981 atttttttga tagaaccatt cataaagcat atcgttctct cagttgttta ttttgtggtg
     2041 atttaatcat acaagaaaac gatatgtttt tttataaaaa catgaatagg aaaaatagtt
     2101 gacaagaaaa tgacaacgcc atatgatgtt ccatagtatt tacaacctgt catagatgac
     2161 tgtaaaaaat aaaagtaaaa agaaaggacg acaacaggtg gatttttatc agtctctaca
     2221 actagatccg tttattttaa aacaaaaaat acgagaagca gcttctaaaa aagaaaagtg
     2281 catgtatatt tgttcattgt ttttacgatc attattcatc gtattgtttg ccatttgttt
     2341 tatcattata attacaacat tatttgaatc gaagcataaa ccatacgctg tggttttatt
     2401 ctgtatgttg atgagtattc gttttgttga ctttggttat aagatttccc attcgatcat
     2461 aggtttagca atcgtcatgt tctcactatt agtagcaccg tatgtccagc tgatcaaatg
     2521 gtcagttatg ggcatgttga ttcattttat tttactaagc agcatattaa tggcaaccgc
     2581 tagtgatccc aaaatgggga atgcttcttt gtacggcttt tcatatctct ttattgttta
     2641 ttcgttacct aaagattcgt tgaacaagaa tttctttaca caaacaagta gcttattatt
     2701 tttgtttttt tgttggtttt cagttctcct ttacagaaaa catcgagaaa agaacaaaga
     2761 caaaagcttg tttaaagaga tttttttgaa aggcatgtat tcgcaggaaa aaatttggat
     2821 gctcatctat gcatttggca ttagtctttt gatcgttgca ggagagtatg tcccttttca
     2881 gcgattgatg tgggcagggt ttgcattttc ttctattgta tctagctatg gattgatgtc
     2941 gattggtttc aaagaacgag cagtagaccg aatcattggc tcattgattg gatgtgtttt
     3001 atttattggt atcagccaat ttattccttt taactgggtc ggtatattag gtggtctagc
     3061 tttaggtgtt tgttctacct atagatataa aacagttttt aattcttttg gtgctttggc
     3121 aatcactgcc agtttattcg gggttccagg agcagtaacg atccgcattt ttgagaatat
     3181 ccttggggta tgtttaggaa tcatgtatat tggggtgact gaaatactta tccgaaaaat
     3241 aagagaaaaa cacggtttga atcattagat gagatcgaat aaaaaataag gaaaaagtga
     3301 agatacacat ggatgataaa ttgctatcat tccatgtgta tctttttacg gtaaatcagt
     3361 gaaacgttcg actttcattt ttaatttata ctttttacgg agttcgttta tttgagccat
     3421 catttcggat ttgtgatgct tttcgattgc ttcttcatct ttccaacggt cgatcagtaa
     3481 aagagattct ggatcttcta gtggaaagta atagtcatat ttctcgtttc cttcttctgc
     3541 acgaatcgct ttgacaatgt ttctttcgac catttcttct gcaaattttc tggcttgtcc
     3601 atttgtactt gtatacaaaa tattcactgt gatactcact aaatcccctc ctgaaaaaat
     3661 tctatcacta gtatatcacg gtgccagaaa ttattattca agatatgata cacatttttt
     3721 ccgcaatgct acggaaatat atattttgaa tagaaaacgc ttacaagata tgcttggttt
     3781 ataaaaataa aaggagtgat tgatttatga aaaaagcatt gattatttgt gcggcaggaa
     3841 tgtcatcctc gatgatggcc tcaaagacaa cggatttctt taagcaaaaa agagaagagg
     3901 tactcgtaga tgcagtttca gctacagaag gcgataatat gattaagaat agtgattttg
     3961 atttatttct gatcagcccg caaacgacga tgtttttgga taaattcaaa aaattaggag
     4021 aatctgttgg aaagcctgtt gttagtatcc ctttccaagc gtatgtccca atccctacag
     4081 gcatccagaa gttagcagag gtgatcgaag agaacatatg atggaatagg agtggatgat
     4141 aatgaaacag aaggaaaaat tgctgttgag atatttgatt gatcacaaga aggagtatgt
     4201 caccagtcaa cgtttagctt ctgaattgtt attatctgat cggacaatcc gtaattactt
     4261 gcagcggatc aaagagttag tagaaaaaaa cggaggtaag atcattgcta aaccaggtta
     4321 tggctaccaa ctgcatattt tacaaagact gacatttgac ttgtttttga gtcggcaaga
     4381 aatagtacct ggatatgcta gagaaccgca aggattttat gagtccgaag atcgacaaaa
     4441 gtatatattg aaccaattgc ttttggaaga tgccattctt tttatggatg atttggcaga
     4501 agagctttat atcagtcgtt ccagtttgac aaaggacatg caagaaatca aggagcgatt
     4561 gattccatat tcactaaaaa tcgtttctaa gcatggtcaa gggacatgga tcgaagggga
     4621 agaaagaaat cgacggcatt ttatcatgga tacttttttt ggaaaccatt atggaaattc
     4681 attgaaagaa tatttagggc aaagccgttt tttcccagac atcagttttg aagagctagt
     4741 cattatcatt ctcgatgaaa caagagaagc taagttaaaa gtttcggatt ttatcataca
     4801 gaatctgact ttacacttgg ctttggggat caaacggctc cgagaagggt ttgaaatcaa
     4861 ggaattaggc atcgaacaag aagtttctaa gcgagtagag tatcaagtgg cgcagcggat
     4921 cgttcaacgt attgaggtaa tcgcaggtgt atcgtttccc gaagaagaaa ttgcttattt
     4981 gacattacat ctcatggcta agtcgaatca caaagaaaat caaaatgatc aggaactatc
     5041 aaaggaatta gcgaaagttc tgaaaagatt gtctcaggaa tttcattttt cattagtaga
     5101 ggatctccag cttttcaatg gactactcga tcatttgtca ccgatgatga ttcgattgag
     5161 ccgaggtatt cgcattgaga acccattgac tgaggagata aagaaagaaa atcccaaagt
     5221 ctttgacgca gtaaaaaggc atttcagcaa catgccggct ttgaaaaact ataccatcaa
     5281 tgaagatgaa tgggcctatt tggctttgca tctcatggcg gcattggaaa aagaaagagc
     5341 tgcgcataaa ctacatgcat tgattatctg tgcaacgggt tacggcagtg cccaactatt
     5401 aaagaatcgc gtagtcagtg aattcggtaa aaatattaca gtggtaagtg taaaaggcta
     5461 ttatgagatc aatgaaacaa ctttaaaagg aatcgacctg attatttctt ctattgatct
     5521 ttcgacaatg tttttcaaaa ttccagtttt acatgtcagt atttttttga atgaagatga
     5581 tgttcgtaaa atacgaaagg tagttggaga atctataccc tatcattcct cagatagacc
     5641 gataccagca ttttctctac agcaaaaaaa gcagatttac accgaacagt tgtctgaaaa
     5701 atttttcaag acttatacgt acgaaccgaa aaaggaagaa attcttcatg atttgctgca
     5761 aaagctgagc ataaatgaag aagaaggata tataagagaa atgcgaaagc agatccagca
     5821 acgtgaaaat atgggacaaa tcatattcag tgatacagtg gcagtcccac atcccgctgt
     5881 tccagttgga atcgaaacaa aggtagccgt tgcattgatt ccttctggga tgcagtggga
     5941 tcaatatcaa gaaattcatt ttgttttttt agtttcgcct tcttatgtcg aaaatgaagg
     6001 aattacggtc gtgactaaag caatcgttag gttagttgac cagctaaaag tacagcagga
     6061 aatcttagct gaacctattt ttgagaattt cagcagtaaa tttatcaaac tgatcgaata
     6121 aggaggaaaa cagatggctg aaaaaataaa cagtgaaaca ctacaagtga cttcttttga
     6181 tatcattttc catagcggaa atgcacggac actgatacat gaagcatttc gctcgatgcg
     6241 taaagagatg tttgaagaag tagaagagaa gttgcgacaa gcaaatgaag agattcttca
     6301 tgcgcatcag tcacaaactc aacttctgaa agaatatgcc agcgggacaa aaatagaaat
     6361 ggaaattatc atggtccatg cacaagatca tctgatgacg acgatgacat tattggaagt
     6421 tgcacaggaa atgaattatt tgtatcaaaa aaataaatag aaaggaagga tcattgtgca
     6481 ggatacagta aaaaggaaaa tcgaattaga aaaaaagcaa cttagtttga aaaacatgta
     6541 tttcaatcgt tatctaatcg tacgttacct gactgccttt ttctttttca tgaacatgca
     6601 atggatgatt ctgcttctat cagctaaaag tttaggaagt agtttaccga tgattctgat
     6661 tttagctatt ttgcctgcag taggagaaca agtaaagctt tatcgaaaac accaaacgaa
     6721 cgttccatgg acaaagtggt attttttgtt ccagagtgtc tttaatttat tagtgattcc
     6781 agtgttgttt acctcaggat ttacattctt gtatcctttt atggcaaata acaatggagg
     6841 acagttattt gttttcattc ttgttgtaag cggtattttt gccagtgcat ttatacagta
     6901 tcgtttaaag aaaatttcac tcaaccaaga ccaacagtat atccgtatta aacaatatga
     6961 aaaagcgtta tatttaggaa aggagaataa ctaatgtttg attttttgca gaaatacctg
     7021 atgggaccta tggggaaaat tgcgcaactt aaaatcgttc gggctgtcat ggcagcgggg
     7081 atggcttcta ttcctttcac gattgtaggc tcgatgtttt tggttttaaa tattttgcca
     7141 ttaccgttcc catttttaga aggatttttt aatgcaactt ttttcaaagt tagtgacttg
     7201 tacatgatcg tcaatacaat gacgatgggg attttatcta tttactttgc cattgtgttt
     7261 gcttatgaat taacgtcaat tgaacgagat gaacaaaaat taaatgttaa tccgctcacg
     7321 ggggcattac tatctgtttt tgcattcttt atgtgtatcc ctgaattgat catgactgat
     7381 ggtaaaatca gcttgattgc ttctatcaat gagacagaaa ctgtagtaaa tggtattagg
     7441 atgggctctt ttgtcgaacg tttaggaact tcaggaatct ttacagctat tattatgtcg
     7501 attattgcgg tcgaattgta ttgtatgtgt gtcaagagaa attgggtcat aaaaatgcca
     7561 gatgttgttc cgccaggagt ttctcgttct ttcactgctc tgatcccaac atttgtcatt
     7621 gcgtttgtgg tgatgatcat caatggatta ttagttgcac taggaacgga tatcttcaaa
     7681 atgattgcta tcccatttgg gtttgttacg cagttgacga atacatggct tggtgtcatg
     7741 gtgatttatt tcttgatcca tgcattgtgg atcgtaggga tccatggagc gaatatcatc
     7801 acttctttct tgaccccgat cgtattagct aatatggcag ctaacgcaca aggcgcaaat
     7861 tatccgttgg caggagaatt caacaattct tatgtaaccg ttggtggatc aggtgcgact
     7921 ttaggattga tcatctttat tgcattcctc gcgaaatctg atcagctgaa agtgttagga
     7981 aaagcctcac ttgtaccagg attattcaat atcaatgaac caatcatttt cgggatgcca
     8041 atcgtctaca atccctattt agcggttccg tttttcttag ctccgatggc ttctgctaca
     8101 ctcgcttatt ttgccattcg gctggaaatc gtcaaaccga tgcttgcaca gatgccgtgg
     8161 ccttcaccag tcgggatcgg agcatttgtt ggtagtggtg gtgattggaa agctgctgtg
     8221 ttagcagtca tttgtgcaat catcgcattc attatctggt tcccatttat caaattctat
     8281 gataataaat tattaaatga agaaagagaa aaggcagcag aattagctag tgaaaagtta
     8341 aaatgatttc taagaaaaaa ggaactgtga caaaaaatca cagtcccttt ttttcttaga
     8401 aatgggaaac cactagtata taaataccat tattcttcaa tcaaccgctt cacgattgca
     8461 taatcccctt cataaggttc atctacacca ttgatttttt gatagggatc cacgcctttt
     8521 cccgcaataa cgactgaatc ttgcggagcg ctttctgcta aagcgcgtcg aatcgcttca
     8581 ggacgatcca tttcataatg aacgatcaaa tctggattag taattgcttc attgatttct
     8641 tccgcgattt ttttcggatc ttcaaatgct gggtcatctg ccgtcaaaaa ggcgatgtca
     8701 gctgtttctg atagcacttt tccaaaatca tgtcgacggg agattgcttt gtttcctggg
     8761 ctgcctaata caacaattac acggccatct ggatgttcat ttttggcaaa tttcagcaat
     8821 gttttcaagc ttagataatt atgtgcatag tccacataga catgcgctcc attggattgg
     8881 atcaattgat ccattcggcc tggtacacga gcttctttta atccgtcttg tgcatcttga
     8941 ctggaggcac ctgttagtgc tgcggcaatg atcgcactgg ctgcattttc ttggttgaat
     9001 cctccagcca gcaaaatatc ataagcagta tcgcagatag ctaagcgatc ttcatgagaa
     9061 gataaagtaa aaccgtgagt acctttatct gaacggatca cttgataatt gctttcttcc
     9121 gaacgaccat aagtgatcgt tggaatctgg aatagatcgg ctgtttccct aagtaaatca
     9181 aaatagtcac tttcatgatt taaaacaact gtttttgaat tcaagatcaa ttgtcgcttg
     9241 caatagaaat agtcatcaaa tgtcggatgt tcgatcggac tgatatggtc aggcgagata
     9301 ttcaagaaga ttccgacatc taaagtcaag ccgtatacgc gttgtgtttt gtaagcttgt
     9361 gaagaaactt ccatcactag atgagtcata ccattttcaa ctgcttctga catcatacga
     9421 tataaatcta atgactcagg cgttgtcaaa tgggatttga aatatgtttt gccatctaac
     9481 gttgtattca tagtagaaag caatgctgtt tttttatttg ttgtgtgatc caagattgct
     9541 tttgtaaagt acgcagcagt tgttttacct tttgttccag tgaatccgat cactttcaat
     9601 ttattttgcg gatagtcgta aaaagccata gcaagaagcg ccattgcttt acggatatcc
     9661 gttacgatga ttcctactgc tgaattttcg tatggttgtt ctgatacata atattgaata
     9721 ccctgttcga ttgctgaatc caaatattct tctttgaaat tcattccttt acagaaaaac
     9781 aaagtagaag catcagcttt tcttgaatca taacttaact gaagaaattc agctttttct
     9841 ggtacatcaa gatgccagcc tcttgttgaa acaaattctt ttaatagatt ttcttttaat
     9901 agtaaatgac ggatgtcttc taaagagaag ctcattgatt gatccctcgc tttattcaat
     9961 ttcttggttt attatagcac actcttttta atatatcgtt tgtttccgct atgacaaaat
    10021 ccagctttat tcaaattttc ttgacacagg ggacaagcgg atgagtattt tcataaacaa
    10081 tgaactgact gtaatagata ggagaacgaa tagtgtctcc aacaatccaa aaacataata
    10141 attcaaaaag acgataagac tattggtaag taaaagtcct tgcggcattg gaagccgaaa
    10201 aaatttgtgg aaaataatcg gcggtatcgt tataccgcca tttgaggccc cgactgtgta
    10261 caatgtgtaa accccaagac tgaacaaaag acttcctgaa atcagtgaaa agaaatgtga
    10321 ggagatcatt tccacttttg gaacaatctc taaaacaaca ggaaaaagca gacttcccgc
    10381 tacagtctta aaaaatactg attttcctaa taggacagcg gctagaagca gcatcataag
    10441 attgatgctc caaacagtaa ctgagcgttg gatagaaaag gccgtttcaa ctaaatgtcc
    10501 gatggcgccc actccggcgg aagctatatc gtgagggagc aagaaccaat tcaatccaac
    10561 tgtgataaag aaaagaccta aaaatataac gggtagtttt tttatttctt gtgtcattga
    10621 tatctcctat aaatgttagc cataaaaggc tgtatatttc tttactttac caaaaaaacg
    10681 aaaccgaatt caaaaaaaca attgttctta taaaagttat ataaaatcga ttgccagttt
    10741 ctgttttacc aattaaaatg atagtaatgt tttgtagaaa gttggaatct gaccgatgga
    10801 taatcaaaga aaaccagcat tagatcaatt aacaccgcaa gaaaaaatgg cacgaggctc
    10861 tgcttggatg accgccagta atatcatttc aagattgcta ggcgctgtct atatcattcc
    10921 ctggtatgct tggatgggcg aaaatgctaa ggctgccaat ggattattca atatgggata
    10981 caatatatat gcattgtttc tactgatctc tacagcagga atacctgctg cgattgcaaa
    11041 acaaactgca cgctataatt cattgaatga atatggcact tcacgacgat tatttatccg
    11101 tgcattacag atgatgggcg ggttaggcct gttgtttgct ttgttcatgt acatcgcctc
    11161 tccttggcta gctcatgctt ctggtggcgg agaagaactg attccaacga tgcgttcttt
    11221 gagtatcgct gtattggttt ttccttgtat gagtgtgata cgagggtatt tgcaaggcaa
    11281 tcaagaaatg atgccttatg cgctgtcgca gattgtagaa caggtggcac gtatttttta
    11341 tatgctattg gctacattca tcatcatgaa agtcatggat ggtgactatg tgacggcggt
    11401 cacgcaatca actttcgcgg catttatcgg tatgctagca agtattcttg tccttttgta
    11461 ttttttgaag aaacatcaag cgtatacatc ggcgtttatt cactacagcg aaaacaaaat
    11521 aaatattgca acgaaggaat tgttgttaga taccgttaaa gaagcgattc cgtttattat
    11581 tgtgggttca ggggtaacga tcttcaaatt agtcgatcag ttcactttta tgaagatcat
    11641 gtctgcatcc accgagtatt ccaatgcaca gttactggat cttttttcga tttttagtgc
    11701 caatccagat aagttgacga tggtagttat tgcattggca acatcgatcg cgtctactgg
    11761 gttaccgttg attacagaag ctgttacgtt gaaagatcga gtaggtctgg caaaattgac
    11821 aagcggaaat ttacagttgt tttcattttt tatgtttccg gcgacatttg gtgttatgtt
    11881 acttgcctat ccgctaaata cattatttta tacaccggat tctctaggaa gtaatgtact
    11941 gatccaagca agttttgtag gactcttcct aggactatat atgctagttt ccaacatgct
    12001 gcaaggaatg tttgaaaaca aggcggcaat taattatttg gttattgggt ttctagtgaa
    12061 actccttctt caatacccag ccattcgttt atttgaagta tacggccctt tattggcgac
    12121 aatgatcgga tttgctgtgt cttgtacctt gatcttaaag agaatccatg aagtagcccg
    12181 ctttaaacca agttttgtct ggcgcaggac attgctgatc tttattctta ccttgattat
    12241 gcttttggct tcgtggatca caaagatgat ttttggtgct tttttaaacg aagatagcaa
    12301 attccagtct ctattgttga ttcttgtagt agcaggtata ggggctttag tgtatggtta
    12361 tttagctttg aaaatccgct tggcagaaag actactagga ccaggaatag cgcgtttacg
    12421 acataaatta aaaataaaat aagtgaatca gaagagctag attgatgctc ttttgaaatg
    12481 ggggaaacgc atgcgtttag ataagttttt agcagatatg ggacttggca gccgcaaaga
    12541 agtcaaagga ttaatcaaaa aaggaacgat cgaagtcaat ggacaagtgg tgaaaagcga
    12601 taaagcgcaa gtaaatgaat tcgaagatgc ggttacttat ttaggagaaa tcattacgta
    12661 tcaaaaagat ttttactaca ttttgcataa acctgcagga gtcatttctg ctactcagga
    12721 taatcatgat caaaccgtaa tcgatttatt ggaagaccag gattatcggg aagatctttt
    12781 tccagtcggc cgattagata aagacacaga gggcctcttg attttaacga atgacggggt
    12841 gtttgcccat cagttactat cacctaaaaa acatgtggag aaagaatatc tagctgaggt
    12901 tagcggtata atgacagaag aagatatccg tttatttgca gaaggactga ttattgatgg
    12961 cgatgagcag acattgccgg caaaactcca aattgatcaa gtagatcaag agacagaaac
    13021 ttcaattatc cgtctgattt tgcatgaagg aaagtttcac caagttaagc gaatggtcaa
    13081 agcagtagga aaagaagtct tatatttaaa aagaatccgt atgggaaaat tttggctgcc
    13141 taaagatttg aataaagggc aatatcgacc catgaccaaa gaagagctag aacaagtgag
    13201 agaaaaataa ttgaatggat agctaaaaga ataagcagag tagttgtagc gccatctgaa
    13261 aagcagactt gtccgtctga ctttcggatg gcttttttgt gaaatttcgg cttatttccg
    13321 tagaagctac ttctggaaca ccgtttgttc ggaataaggg actgtgacaa gacttttgtt
    13381 acagtcccca ttgttccttg tgactcagag ttagatagta aggacacgat ctccctaata
    13441 ctcaggccaa aacgctgaat tcacaacctc tgataaacgg tgttcaatca gctgaccctc
    13501 ggtattagct caaaaaacgg caaaatatga ggaactattt tcccgttttt ttctctaata
    13561 ctcaggccaa aacgctgatt tcacaacctc tcctatactt tgtttcattt tccctatact
    13621 ttgtttattt attctcatta ttgaaagcgc tataattacg ttgcgaatga atcgaaaggg
    13681 gtttttagat gaaaaaacga aaggggttca atcatttttt tatgaatcta tggcgttata
    13741 aagcacttgt cctgatggct gtaccgggta tgctttggat gatttttttc ttttacatac
    13801 ctgtaatggc gaatgtcgta gcattcaaaa attttcatat ttcagcagat gggttcttgg
    13861 ctagcttgag ggaaagtcct tgggtgggtt gggaaaattt caaattttta ttttcttcgg
    13921 atcaagcatt tctcatcact aaaaatacaa ttttatataa tgtagcattt atccttttga
    13981 atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat aaacgactcg
    14041 ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc attatcagtt
    14101 actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg atcactggac
    14161 atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt atcttggtgt
    14221 tcattggtac atggaaaggg attggttata acagcattat ttactttgca tctgttatgg
    14281 gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa tggcagcaga
    14341 tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg attttagccg
    14401 taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg aattccggtg
    14461 cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg acatccacag
    14521 gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc tttgtcttgt
    14581 tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta ttctaaggag
    14641 gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca ataagacggc
    14701 taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt tgccatttat
    14761 atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg ggtacagttt
    14821 ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga tgcaggataa
    14881 aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac tgatcaatag
    14941 cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat tccgtcgttt
    15001 ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg ccaattatct
    15061 tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct tgccaatggc
    15121 tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg tctctgattc
    15181 gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg ttcagatcgt
    15241 tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat tgggttactg
    15301 gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc cactacagta
    15361 tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag gagcaggaag
    15421 ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg cgatcgtagt
    15481 catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt ttgtcaaagg
    15541 tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata aaattaggag
    15601 gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg ttgttgttgg
    15661 gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct agcaattcgg
    15721 acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa ttgatggaag
    15781 tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat tacattggct
    15841 ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat tatgatatcg
    15901 cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat ttgactgaat
    15961 tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat attaaaggaa
    16021 atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt gctcaacaag
    16081 tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat ggaatccaat
    16141 cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca aacacggcag
    16201 cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct ttagggaata
    16261 cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac caatacgata
    16321 atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa ggactcattc
    16381 caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact tggctaatgc
    16441 gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat gcagcaaaac
    16501 aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa gcacaaatgg
    16561 caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa cttctagggc
    16621 tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag ggagaagcat
    16681 gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat cagccaaata
    16741 cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa gagtctatta
    16801 cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact tcaccgattt
    16861 taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt tctaatgtca
    16921 tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa acattaccga
    16981 aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa atgcaaaaac
    17041 aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg tgacaaaaga
    17101 cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct cggtattaag
    17161 ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac tactcgaaac
    17221 taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc ctcggtattc
    17281 gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta ataatcaggt
    17341 caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact taatgtttgg
    17401 aggagaagat atgttaggga aggttttgga agagttattt atccggatct gggtcattat
    17461 aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct ttggattagg
    17521 tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt ataaagaaat
    17581 cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca atctgctatt
    17641 tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat ctgttcaaat
    17701 caaagggctc atttttttag ctattgattt tattttactt tttgccttgc tctgcctatt
    17761 tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg ttcctaatct
    17821 gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa aaataattat
    17881 tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc tgtttggtgt
    17941 cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg aagaattaga
    18001 taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga accttactaa
    18061 atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact gctaccgtta
    18121 tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg acaagtgatg
    18181 ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg atcatcaaaa
    18241 accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta acaaaaccca
    18301 tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa ttagtttctt
    18361 tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg atatatatcg
    18421 tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt ggggaaataa
    18481 aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc acacaaggag
    18541 gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat atgattttag
    18601 aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg acaaatcgac
    18661 tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa gaaatgatca
    18721 tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa aaaaacaatt
    18781 ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta gataaaaaaa
    18841 gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc attattttag
    18901 acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag caagtatcta
    18961 tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg atcgacataa
    19021 caaacacgga atatgaactg agggaacttt caatcggtat caacgaaatg ttggatagca
    19081 tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac gcacatatgc
    19141 gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa tatatccgaa
    19201 tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg ttttccgctt
    19261 tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa ctggactttt
    19321 gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc tatcatttta
    19381 tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag ccattagttg
    19441 aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta agtgtcaaag
    19501 tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa gggatgactg
    19561 aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa cttcacggtt
    19621 ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc agttattata
    19681 tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc aaggagggat
    19741 gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg ggttaaaaaa
    19801 gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga atgccaaaga
    19861 aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg tgacaatgcc
    19921 agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata attttgaatt
    19981 tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc aattaggcgc
    20041 cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc tcaagaaaat
    20101 caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct atcaagaact
    20161 gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag aaataatagg
    20221 acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa ctcatgatac
    20281 tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac gaaattacgg
    20341 agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct tctgttgttt
    20401 tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag agacagattc
    20461 tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt tgcatcaatt
    20521 ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa aagatcaaac
    20581 catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag tcgctcaaaa
    20641 aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg aagatatacg
    20701 atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc tggaagatga
    20761 ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag aacttcagca
    20821 gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg acaatgtgga
    20881 aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca aagaagtgtc
    20941 agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag aaacgaaaaa
    21001 aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac tgcttttgca
    21061 cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca ccacgtattt
    21121 ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag aaaacagtgg
    21181 agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg tctagaaatc
    21241 taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc tctcgataaa
    21301 cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc
    21361 attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga cctctccact
    21421 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa atatgaggaa
    21481 ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac aacctctcaa
    21541 taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga aaaatatgag
    21601 gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
    21661 tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt cttagggtga
    21721 gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg atgggtaaaa
    21781 tataatttcg tatattctct gtccatccgt ttgattttat ggataaattg ttgctgcggc
    21841 cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa aacagccttg
    21901 tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg ataaaatccc
    21961 agactattga agatatacca agcggccata cttccattgt cctcatctcc agggtaaccc
    22021 attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg tgccatttca
    22081 ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt tgaaatagct
    22141 aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa accatatcct
    22201 cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt atatctctca
    22261 ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa gctgctttgc
    22321 caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg ttctctgaac
    22381 ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat attttgatag
    22441 ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac cttggcaact
    22501 tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga ttcgtggtag
    22561 tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc ataatttgga
    22621 tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg catcaagtct
    22681 cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt cccaggcata
    22741 agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct atagctgttc
    22801 aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata aagaggataa
    22861 accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg ttttatagat
    22921 cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata aaatgtttgt
    22981 ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg ttgatcatgg
    23041 tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg agcaaggtaa
    23101 gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc cgagctgata
    23161 aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa cgggatcgag
    23221 tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc aaatctcaat
    23281 ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat ttttccttcc
    23341 actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa aagtaaaccg
    23401 ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg tatcaatgta
    23461 gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa tatactttct
    23521 tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt tccattgatc
    23581 ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg ggtaagacgg
    23641 tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg atccgtagtt
    23701 tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca gttcccatta
    23761 gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat attcatttta
    23821 tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat cggatggtcg
    23881 atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta actgttcaaa
    23941 atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga tatcatcaaa
    24001 tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga gattcatggt
    24061 ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact tagtgatata
    24121 agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga aagtcaaacc
    24181 agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt gtggatgagt
    24241 ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat aagaggcagc
    24301 aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact catagatttt
    24361 tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta agtcaagatg
    24421 ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta gctcttttgc
    24481 tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt tatcttgcat
    24541 cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat atggaagaag
    24601 aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag aagcggattt
    24661 aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta attcagtcaa
    24721 tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt tttcagttgg
    24781 tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat cattaaatat
    24841 tgtctggtac aatggttgag tcatataaac acctcttaat gatatatcat attcaaataa
    24901 taccataatg gatgaaaaag taaataaaaa gatatttttt gaaagcgtag acataattgg
    24961 ctgtatgaag tataatcttt aaagatatat caaatagaaa gaaggtttta ttagtggttt
    25021 ataaagatat accaaaatcg gtgcagaaat ttatggaaga tatcacacaa aaatgtgggg
    25081 atatccatgc ggactgggca aagaatttta cagcagcatt tgccaatact ctgttgacaa
    25141 cagtcaaaaa gcaaaatgac ggaaccacct ttttattgac gggagacatt ccagcgatgt
    25201 ggttaagaga ctctactgca caagttcgtc cttatttagt gattgcaaaa gaagatgagg
    25261 aattagctga catgatcagc gggttagtca aaaagcaatt tttttatatc aatatcgatc
    25321 catatgcgaa tgccttcaac gaagaagcta acggtgctgg ccaccaaaca gatcatacaa
    25381 aaatgaacga ttggatctgg gaaagaaaat atgaaattga ttctttatgc tacccaatac
    25441 agttggccta tcttctttac aaaaatacag gaaaaactga tcaattcaat caatctttta
    25501 ttgaaggtat caaaaaaatc ttggacgtgt tcaaaacaga gcaagatcat gaacactctc
    25561 cttatacatt tgtacgagac acgacaagaa aagaagatac gctgatcaat gacgggcgag
    25621 gtgcaaaaac agcttttaca ggaatgacat ggtctggttt ccgtcctagc gatgatgctt
    25681 gccgttatgg atatctggta ccatccaata tgtttgcggt ggtcgtgtta ggttatgtgg
    25741 aagaaatttt tcaaacgata ctcgatgata aagaaattgt acagcaaaca cagaaattga
    25801 agaaagagat ccaagagggg attcagaatc acggacgaac ggttaatcaa aaagggcaaa
    25861 cgatttatgc atatgaagta gatggaatgg gaaacgccac agtcatggat gacagtaatg
    25921 tgcccaatct gatcgccgca ccttacttgg gatattgttc tgaacaagag gaagattatc
    25981 tgcaaacaag aagaacattg ttgagtaaag aaaatccata tttctatgaa ggaaagtacg
    26041 caaaaggaat tggatcctcc catacgccag aaaaatacgt ttggccgatt gcattagcta
    26101 tggaaggaat gacaacgtca gacaaatctg aaaaagagcg gatattgaat ttacttgtag
    26161 caactgacgc aggaacacac cttatgcacg aaggattcga tgtgaacaat cctgaaaatt
    26221 atacgagaga atggttttct tgggcaaata tgatgttttg cgaattagtc atggattact
    26281 ttgatattag agtagaaaag taggaggtat cgtaaatgaa aaagaaagta tatatcattt
    26341 ctcatagcca ctgggatcgt gaatggtaca tggcttacga acagcatcac atgcgactga
    26401 ttgaattgat agacgatcta ctagagctat ttgaagttga tccgtccttc aacagttttc
    26461 atttggatgg gcaaacgatt attttagatg actatcttca agtaaggcca gaaaaaagac
    26521 aggcaatcca acaagcaatc aatgaaggaa aattgagaat tggaccattt tatatcttgc
    26581 aagatgattt tcttatcagc tcggaatcga acgtgagaaa tatgctgatt ggcatggaag
    26641 aaagccgtaa atggggaacc cctgtcatgt taggctattt tcccgataca tttggaaata
    26701 tgggacagac gcctcaatta atgaaacaag ctggtatttc agcagcagcg tttggccgtg
    26761 gtgtaaaacc aattggcttt gataatcaag ttttggaagc agaaaactat tcttcccaat
    26821 actcagagat gtggtggaaa ggccctgatc aaacagcaat ctttggtctg ctatttgcca
    26881 attggtatag caacgggaat gagattccag tagaaaaaga agcagctctt gccttttgga
    26941 agcaaaaatt agcggatgca gaacaatatg cctctacgaa ccacttattg atgatgaatg
    27001 gtgttgatca tcaaccggta caaaaagata tatcaaaagc aatccatctc gcaaatgaac
    27061 tgtttccaga ttacgagttt attcattcta attttactga ctatctggaa gcagttcaaa
    27121 aagatgtgcc agaagactta ggctcagtag aaggagaatt gacaagtcaa gaaacggatg
    27181 gctggtatac tttagccaat acagcatctg ctcgggttta cttaaaacaa tggaatacca
    27241 aggtccagcg acagctagaa aatattacag aaccacttgc gacgatggct tatgaagtta
    27301 gtggaaacta tccccatgat caattagatt atgcttggaa gacattaatg caaaatcatc
    27361 ctcacgacag tatttgcggt tgttcagtcg attctgtcca tcgggaaatg atacctcgtt
    27421 ttgaaaaagc agatgaagtt ggaaagtatc ttgcacaaga ttccttagaa cagctgaccg
    27481 cagcaatcga tacgaccgga ttcccaaaag acagctttcc atttgtcatt gtaaatactg
    27541 caggaatgga taaaactggt gaagcagaaa taaccatcga attagaaaga aaaagattcg
    27601 ctgaaggaat acctgaacaa ctatatcaag agttagaaaa tttgcctaaa cgtaagtatc
    27661 atgtagaaac aaaaagtggg gccactatac cagctatttt atctgaagaa actgtccaat
    27721 ttggttatga tttgccgaaa gaccgtttcc gtgtgccata tatggcaaga atgatcaaag
    27781 tgactttacc attagaaaac atgccagcat tttcttggga aacattcgcg cttgtagaag
    27841 gggaagcgaa agcagaagaa aaggaaacaa tgattcatca gagtggacga atcatcgaaa
    27901 acggccctct acatttgacg attgaaaaaa atggtaccat tacaatggaa gatcgcaaaa
    27961 acaaacgaaa gttgaataat ctgcatattt ttgaagatat cggcgatata ggaaatgagt
    28021 atatctttaa acaacctttc tgtgatcagc caatcctatc cagtaacaaa gaaaactctg
    28081 aagtaaaagt attagtagat acaccggaaa tagcaaaaat aagtattcta caggaaatgg
    28141 aaatccctgt ttctgcagac gagcgtttag aaaaagaaca gcagatggtt gtggaatttc
    28201 gttatcgaaa agcagaacga tccaaagaaa aacgaatcct tcaaataaaa acaatcatga
    28261 caatcagaaa agatagtaaa aaaatcgact ttgaaacgac tctcgacaat cagatgaaag
    28321 atcatcgctt acgtgtcttg tttcctacaa agttacatgt ggaacatcac gaagcagata
    28381 gtatttttga agtggtgaag cgcccaaacc atgtgtctaa aagttgggaa aaccccacga
    28441 atccgcaaca tcagcaagca tttgtcaata ttcatgatga agagtacggt gtaacagtcg
    28501 gaaactttgg tttgaatgaa tatgaagtaa cagaagacgg acaaatcgca gtgacgctac
    28561 ttcgcagtgt gggagaacta ggcgattggg gatattttcc aacaccagaa gcacagtgtc
    28621 taggtgagca tcggttcaat tacagcattg aactacatgg accagaagaa aaattctcta
    28681 cttatttgca tgcatacgca gcacagattc ctttttccac tcaacaaatc aaacatcatg
    28741 aaggaacatt gatttcaaaa caacaatatt taacgataaa aagtgaaacg tttgcaatta
    28801 cagctttgaa acgcagcaag tttagtgata aagtagtagt aagaggtttc aatatgtcta
    28861 gtcatttaga aaaattggaa ataactaaag acaatggaaa aactgtcata ttgaatctgc
    28921 ttgaagaacc aacgaagcaa gcggttgtcc caataatcca accttatgag attcggacaa
    28981 tcggattcga ggaggaaaac gaatgtatgg cggaatagaa gcaggcggaa caaaatttgt
    29041 gtgtgcggtt tcagatggtg gagaaatttt agaaaaatta agtatcccta ccacaacacc
    29101 agaagaaaca ttggatcagg tatttgaatt ttttgatcgt tatgaattga agggaatcgg
    29161 gattggctca ttcggcccga ttggtatcga caaaacaaaa gataattacg gatacgtttt
    29221 ggcaactccc aaaaaagggt gggcacaatt cgattttctt ggcacaatca aggaacgcta
    29281 caatattcct gctgcttgga caacggatgt caatgctgct gcatatgggg aattgaagca
    29341 aggagcagca caaggaaaaa atagttgtat ttaccttacc gtgggaacag gaatcggtgc
    29401 aggtgtcgtg atcaatgaag aaattttttc agggatcgca catcctgaaa tgggacatat
    29461 ttgggtgaaa cgccatccag atgaccatta tgaaggtact tgtccttatc ataaagattg
    29521 tttagaagga ttagcagcag gaccgtctat tgaggcacgc acagggatca aagggcaaga
    29581 tctgccgcaa gatcatcctg tttgggctat tcaggcttat tatattgctc aagcactgat
    29641 caactatacg ttgacgttag cacctgaaaa gattatttta ggcggtgggg taatgaatca
    29701 ggatcatctc cttcaaaaaa tccgtcagca atttgttgaa ttgatgggtg gttatatgga
    29761 aacacctcaa gtaagtgaat atatcgttcg ttggggactg ccgaatgaaa gtgggattat
    29821 cggtagtttg ctgttagcag aacacgaaca tcaaaagtaa gcagacaaat tacacaaaaa
    29881 gaacagagtc ttatcattat ctgctgataa gactctgttc ttttttattt ctattcccat
    29941 aatccaggta aagctttacc gactattttt tcttgagtaa atggatggat catgtgcagt
    30001 tctaatgcat ggagcatcag tcgttcagct gttctatttt ggtagagagg gtctccaacg
    30061 atcggatgtc ccttatgagc taaatgaact ctgatctggt gtgtacgtcc ggtttccaaa
    30121 acacaataga cttgtgtttg ttcagaaagt actttgttga cagtgacatg tgtcaaggcg
    30181 ctctgaccat ttttatcatc tacgaccctt tttctccgat catggcgatc tcgtccaatt
    30241 ttattggtga tggtcatatc tttggcaatt tctccccata cgatggcttg atatctccga
    30301 tagatcattt tttgttccaa cattcttcca agtatcggca agacaaatgg attttttgca
    30361 aataaaacgg caccgcttgt ttctttgtcc aagcggtgga caacgtaagg tcgttgctgt
    30421 ttagtgtcga gataagcagc taaatgattg agtaaggtac tgttttctgt cggttcattt
    30481 ggatgagtct tcattcctgc aggcttatcc actataatca aatgctcatc ttcatataaa
    30541 acacggatgt gcttcgcatc tcctaattga acttcttgat aattataatc tgtttcttcg
    30601 aataacaatg tgatctggtc attgcccttt acttcatgat ggaacaaagc aggttgttga
    30661 ttgatccaaa cgtttttacg tatacgcaaa aagtgtcgaa cctttctagg aactaaccat
    30721 tccttttcaa gcaagtcgcg aattgttgtt tcaggatggt tcataggtaa agtgatcgtg
    30781 attttcacaa gtgtactcct ttcagtgcaa ttgcagacta attgtaccat ttttaaaaca
    30841 tatttaagaa aaaacttaca tttttctgtt cattcttatg ttactctact tgcactaacg
    30901 taaaaaaaca tattgaacta caacttaagc aggaagaaaa cactctttca ctatggtaaa
    30961 ataagacgat tgaatcaaat agacaggaga agaagcaaat ggattttcaa agcattttag
    31021 aaaagataaa agctgctttg atcagttttt ggcgttggat cagaccatat ctcggacaat
    31081 tccatcgatg gcgaaagcgc atctggaaaa aatatcatgt gaacaaattg attttgctga
    31141 ttggtttgat ctgtgtctta gtgacaagta tctacctctt tattttagca aaacaagcaa
    31201 atgtcgaaac gttaaaatca ggattaagcc aatcgacagt catttatgat aagaaaaacg
    31261 aagaagctgg cacactgtac gcccaaaaag gaacatatgt agaattaaac gcgatttctc
    31321 cctatatcca agatgcagta atctcgacag aagataggaa tttttacgag catcacgggt
    31381 ttgatatcaa agggattgcc cgtgcagcag tccgaatggt cttgtctggc ggaacatcag
    31441 ggggcggagg aagtacgatc actcagcagc ttgccaaaaa tgcgtatctg acattagacc
    31501 aaacgtttga tcgaaaagcg aaggaattat ttttggcaat cgagatcgaa aagaagtaca
    31561 gcaaagaaga aatcttgacc atgtatctta acaatgccta ttttggcaac ggggtatggg
    31621 gtgtccaaga cgcttccaga aagtattttg gagtagatgc aacgaatgtt acgctgtctg
    31681 aggctgctac tttggcagga atgctgaaag gtcctggtat ttataatccg atcgatcacc
    31741 caaaaaatgc gaatgatcgg aaaaacacag tactaagtgt aatggaagaa aacggaaaaa
    31801 tcacaaaaga acaagaacaa gcagaaagcc agacagatat cagcgcgtat cttaatgaca
    31861 catacgataa ttcagattct ggttatcgtt atccttatta ttttgatgca gtgatcgatg
    31921 aagcagttaa cgagtatggt cttgatgaag aagatgtcat gaataaaggc tataaaatct
    31981 atacagcttt agatcaggat tatcaaaagc aattagaagc tacttatcaa aacagtgcat
    32041 tattcccagc aaatgcttca gatggtgcca tggtccaatc tgcttcagta gctttagatc
    32101 cgaatacagg tggtgtacaa gcactagtag gacgaagagg agatcatgtc tttagaggat
    32161 tcagctttgc tacacagatg cgtcgttcac caggatcaac gatcaaacca ttaagcgtat
    32221 atacaccagc actggaagct ggatacaagc cttccagtat cttgaaagac gaaccgcaaa
    32281 gttattacga tgcgcataat tttgacggga cataccaagg agaagtaccg atgtatcaag
    32341 ctgttgcaca aagtttgaat ttgcctgctg tatggctttt gcatgagatc ggtttacaaa
    32401 aaggatatga taaagcagaa gaattcggcc tgcctttagc aaaatctgat aagtattatg
    32461 gcttagccct tggtggactg gaaaaaggag tatctccatt gatcatggcg ggtgcttaca
    32521 gtgcatttgc caatgatggt cagatgtata cgccgcatct tatcacgaaa atcgttgatt
    32581 cgactggagc ggtgatcgtc gataacacaa acaaaaaacc aaaacaggtc atatcaaagg
    32641 aaacagccga tgaaatgacc agcatgcttc ttggtacatt ttcaaatgga acagcggctg
    32701 ctgcgaatcc tgcaggttat acgatagcag ggaaaacagg aaccacagaa actaatttcg
    32761 atgccacaaa agcaaatgac caatggatga tcggttacac gccagacgta gtgatttcga
    32821 catggatggg ttacgaagaa tccagcaagc tgcactattt ggaagggaca agtggaaatg
    32881 ttgtcgggaa agtcttcaaa tcagcggcag aaggcatcct tccgtatact tcaaaaacag
    32941 gatttagtgt agcagatgcc tatgccacag gaggacaagt ggtagctgca gatcaagttg
    33001 gaaacacagg aaattctaat ggagagtctg gaaattggca agataacttg aatcaatatg
    33061 gtgaacaagc aaaagaaggg ctgaaaaact tcggcgatat ggtgaaagaa ggcgttcagg
    33121 gaattggtga agcggctaaa gacctctggc ggaaatatca aggagagtag cccctttgaa
    33181 aacgaataac cccatgttac aatagagagt aactactaaa agaaaagagg gcataacatg
    33241 tcaaacatct acgatagcgc gaatcaaatc gaacgtgaga tccgtgaatt accagaattc
    33301 aaagcgttga aagaagcgta cgagaaagta caagcaaacg aagaagcaca caaactgttc
    33361 aaagacttcc aagcgatgca aatcgaatta caagaaaaac aaatgagtgg acaagagttc
    33421 agcgacgaag atgcaatgaa agcacaagaa atggctgtga aaatccaatc agaagaagta
    33481 atcaatgact tgatgcaaaa agagcaaggc ttcagtacaa tcatcaatga cttgaaccgt
    33541 atcatcatga caccagttcg cgacctttac agcgaataaa actattttat gaaacaacag
    33601 agggtgtgaa agagacgttc ggccttgagc gatgacaaaa ttgcttctta ttgccgatag
    33661 gctgtctctt gaacaccatt taccaagagg tcgtgagcct accgtttaac tctgagttac
    33721 aaggaacaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact tgtgaccgag
    33781 gagttggcag gagaacaccg tttattcaga aataggagct acgacaaaag tcttgtcata
    33841 gctcctattt tttgtcattc atttttcaag cggtagaaat agcctttttg agctaacagt
    33901 ttttcgtaag tacctatttc aacgattttt ccttcatcga tcacaacgat tttatctgcc
    33961 tctttgatcg ttgaaagccg atgagccaca ataaaagtag tgggtgtttc tgtcaggtaa
    34021 ttcagggctt gctggatttt tttctctgac tggttatcta aagctgaggt tgcttcatct
    34081 agcaaaatga tttttggctg acgtatcaac gctcgtgcaa tggagataca ttgcttttgg
    34141 ccaccagaca gcttgttgcc atgttcgcct actaaggtat ccagaccatc aggaagctgg
    34201 tcaatcagtg aagaaagttg cgcagcttcg atgacttcat ctaaacgttc ttttgatacg
    34261 tttttcaagc cataagtgat gttctctttg atcgaagcgg agaacaacaa agtggtttga
    34321 ggaacaacgg ctaaataatt tcgcacactt cgcatatcca gttcatccaa aggctgatca
    34381 tctagaaaaa tttttcctga agtcggctgg ataaatccaa tcaataagtt aaggagtgta
    34441 gattttccag agccagatgg cccaacaaaa gcaattgttt ctccagcttt cacctctaga
    34501 gaaaaatgat cgataatcgg tttgagtgag tccggataaa gaaaagaaac atcctcaaaa
    34561 cgaaaatttc catccaaatg ttggattttt ttcttaccgt gataagtttc tacatcgcca
    34621 gcaatcaaca gttcgccaat agaagtgata gattccatgc cttttgaaat cgtaggaatc
    34681 agcgtgatca ttccagaaac ggaattgacg atcgtcgtga aatacgtctg atacatcacg
    34741 acatcgccgg gttggatgcg cccttgtaaa gctaatatcc cagtgaaaat caaacaaatc
    34801 agctgaaata tctgaaaact agcccagctg actgatccga aaacagattg caggatatct
    34861 aagtggaatc ctttttcata gatttgagta aaatgcttat ttaagcgagt tgtttcgtgt
    34921 ttttctaacc catgagctcg agcaatcggt gctaattcga tcacttcgat cacttttgca
    34981 gaagtctctt ccatttcttg cctgaagtct gagttggact gtctgatcct tttacggaaa
    35041 aagagagtaa cgaatgcggc gagaggaaca gtcaataaga aaaacatgaa aacaacagga
    35101 cttttataaa gcgtgatacc taaagctacg aataaattga cagcgatatt taatccgctg
    35161 acaaatactt gagaggacaa cgtttggata gcttctacat cacggatgat ctttgactgg
    35221 attcgtccag attgcaattc tttttgataa ggaatagata gttcttggat tttcttgata
    35281 agggtcgctc ttagtccagc ttctacttga cggatcgaag tgctatggta ttttacatga
    35341 aaataattcg tcaatatatt ttgaccaatc agacaagcca tcaaaaaagc gttgaaataa
    35401 atggcctgca actgagaaga atctttcgat gaagctgcat tgattacatt ggcaatagcg
    35461 atcggcatta accaagatgg aagatgtttg ataataaaaa agagtcctga catggcaaaa
    35521 cgccaataat ttccctgata aagatgcagc aatgtctgaa aagcatttgc atgatatttt
    35581 tgataacttt tctgtattcg ttcctccata atcttctcct taacaatctt ggtaaataaa
    35641 ctgcaagtgt tctattgctt caggaacata aatctcagga gtagcttcaa gagtggcgta
    35701 cattagagga cgctcatatt tgatataatg cagtaccggg cggaaatcta aacagccctt
    35761 gccaacaggt accatgcgga tctttccatt ttccatgata tagtctttga gatgaagcgc
    35821 tgtaatataa ccatgtaaat tgtccaaggc acctcggatc actttttcct gatcagcatg
    35881 attttcttta tgcatcaaat tcgcacaatc taaaatgatt tttaaatttg gtgaagctac
    35941 ttcatcgatt aaacgtttag ctaactgata gttatagatt ggatgattga tccctgcttc
    36001 aacagcaaca gttacgccaa aattttctgc atgagcgact aattcaatga tagaatttct
    36061 ggcgatctga taagcttctt cagtaaaatt ttcttttgtg taaccgacag agacactacc
    36121 ggtttccgta gcaatcatct tggcttgata ggcagagcac agagcaattt gctgtttgaa
    36181 agttgccaat gcttgtgcac gaacttcttg atttttgctg gataggttga tatagcatcc
    36241 taaaatggaa agttcgatat tgtttttttg gaagtattca ccaaaatagg cagcactccc
    36301 aagtgtcatg tcagttaatt taagttgttc aggaaatgct ttttgtggtg caaattggat
    36361 atgggacaag tgaagctgtg atactttttg aacgatttct tcacgatttc gaacatctaa
    36421 atcgtgggca cggatgccta aatttaatgg aaacatctca aactcctttt tgtaaataat
    36481 tagcaagagg cagacgggat tcttttatca attcaacaat tattcgggca actttatcag
    36541 cgccttctgg actaaagtgg gtattgtctt ttagtccttc tgggtaattt ttacaagtat
    36601 caggcataag atgcaggtag aatttttttg tctcttcttt tgagaaggtc tggaacaatt
    36661 cttgtgatct tgagaacaca tctaaaagag gaatatggtg tttctcagca aatgcacgca
    36721 tcgcttttgg atagtctcca agtatatctg gattcaatgt tccgttttct aagtaatctc
    36781 gtcgcgtcac agatgtcagt aagaccattt ggacgttcgc tttttttgcg cattcaaaaa
    36841 atatctgcag attttttaaa tagctaccga agggaagcgt acctctttct tcttggattt
    36901 tctggtcgtt atgtccaaat tgtacaaata agaaatcatt cggctgaaac tgtgaagcta
    36961 gacgggctaa ccttccttcc tggataaatg atttggtgct gcgtccattc tttgcttggt
    37021 tattgatctg gatctcgggt gaaaaataag cagagatttt ttctccccaa cctgtctcgg
    37081 gacgcttgtt ttcatctttt tctgccgcag tagaatcacc ggcaatatgg atagtaggca
    37141 tgttatctct tctttcataa aggtttatag gcaatgaagc agaacagcaa gagaagaaga
    37201 cggagcaa
//
LOCUS       DAPCMU010000012        36852 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPCMU010000012 DAPCMU010000000
VERSION     DAPCMU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.012"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(27..1391)
                     /locus_tag="RD744_000495"
     CDS             complement(27..1391)
                     /locus_tag="RD744_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0556526.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1388..2947)
                     /locus_tag="RD744_000496"
     CDS             complement(1388..2947)
                     /locus_tag="RD744_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556527.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3003..5483)
                     /locus_tag="RD744_000497"
     CDS             complement(3003..5483)
                     /locus_tag="RD744_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556528.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5467..5802)
                     /locus_tag="RD744_000498"
     CDS             complement(5467..5802)
                     /locus_tag="RD744_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556529.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5816..6850)
                     /locus_tag="RD744_000499"
     CDS             complement(5816..6850)
                     /locus_tag="RD744_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDU0556530.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6940..7620)
                     /locus_tag="RD744_000500"
     CDS             complement(6940..7620)
                     /locus_tag="RD744_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDU0556531.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7904..9007)
                     /locus_tag="RD744_000501"
     CDS             complement(7904..9007)
                     /locus_tag="RD744_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDU0556532.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9636..10106)
                     /locus_tag="RD744_000502"
     CDS             complement(9636..10106)
                     /locus_tag="RD744_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDU0556533.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10436..12076
                     /locus_tag="RD744_000503"
     CDS             10436..12076
                     /locus_tag="RD744_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDU0556534.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12230..13552
                     /locus_tag="RD744_000504"
     CDS             12230..13552
                     /locus_tag="RD744_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDU0556535.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13620..14054)
                     /locus_tag="RD744_000505"
     CDS             complement(13620..14054)
                     /locus_tag="RD744_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0556536.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14119..14956)
                     /locus_tag="RD744_000506"
                     /pseudo
     CDS             complement(14119..14956)
                     /locus_tag="RD744_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(15525..15830)
                     /locus_tag="RD744_000507"
     CDS             complement(15525..15830)
                     /locus_tag="RD744_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDU0556537.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15855..16286)
                     /gene="ruvX"
                     /locus_tag="RD744_000508"
     CDS             complement(15855..16286)
                     /gene="ruvX"
                     /locus_tag="RD744_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDU0556538.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(16283..16549)
                     /locus_tag="RD744_000509"
     CDS             complement(16283..16549)
                     /locus_tag="RD744_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDU0556539.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16804..17529)
                     /locus_tag="RD744_000510"
     CDS             complement(16804..17529)
                     /locus_tag="RD744_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDU0556540.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17890..17960)
                     /locus_tag="RD744_000511"
     tRNA            complement(17890..17960)
                     /locus_tag="RD744_000511"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17926..17928),aa:Gly,seq:ccc)
     gene            18077..18829
                     /locus_tag="RD744_000512"
     CDS             18077..18829
                     /locus_tag="RD744_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDU0556541.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18845..19324
                     /locus_tag="RD744_000513"
     CDS             18845..19324
                     /locus_tag="RD744_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDU0556542.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19575..21155)
                     /locus_tag="RD744_000514"
     CDS             complement(19575..21155)
                     /locus_tag="RD744_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDU0556543.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(21148..21891)
                     /locus_tag="RD744_000515"
     CDS             complement(21148..21891)
                     /locus_tag="RD744_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556544.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(22067..22843)
                     /locus_tag="RD744_000516"
     CDS             complement(22067..22843)
                     /locus_tag="RD744_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0556545.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(23091..23927)
                     /locus_tag="RD744_000517"
     CDS             complement(23091..23927)
                     /locus_tag="RD744_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDU0556546.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23938..24867)
                     /locus_tag="RD744_000518"
     CDS             complement(23938..24867)
                     /locus_tag="RD744_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDU0556547.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            25060..25413
                     /locus_tag="RD744_000519"
     CDS             25060..25413
                     /locus_tag="RD744_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDU0556548.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(25487..26143)
                     /locus_tag="RD744_000520"
     CDS             complement(25487..26143)
                     /locus_tag="RD744_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDU0556549.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            26369..28303
                     /locus_tag="RD744_000521"
     CDS             26369..28303
                     /locus_tag="RD744_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0556550.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(28389..29612)
                     /locus_tag="RD744_000522"
     CDS             complement(28389..29612)
                     /locus_tag="RD744_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDU0556551.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29606..31504)
                     /locus_tag="RD744_000523"
     CDS             complement(29606..31504)
                     /locus_tag="RD744_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDU0556552.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(31498..32616)
                     /locus_tag="RD744_000524"
     CDS             complement(31498..32616)
                     /locus_tag="RD744_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDU0556553.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32699..33532)
                     /locus_tag="RD744_000525"
     CDS             complement(32699..33532)
                     /locus_tag="RD744_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0556554.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33525..34442)
                     /locus_tag="RD744_000526"
     CDS             complement(33525..34442)
                     /locus_tag="RD744_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0556555.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(34465..34644)
                     /locus_tag="RD744_000527"
     CDS             complement(34465..34644)
                     /locus_tag="RD744_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556556.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(34669..35793)
                     /locus_tag="RD744_000528"
     CDS             complement(34669..35793)
                     /locus_tag="RD744_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0556557.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35976..>36852
                     /locus_tag="RD744_000529"
     CDS             35976..>36852
                     /locus_tag="RD744_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0556558.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGL"
BASE COUNT        11177 a         7339 c         6312 g        12024 t
ORIGIN      
        1 cacacaataa aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct
       61 ctcttagcta gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca
      121 gacagaaatc caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata
      181 tccgcaagca ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt
      241 atttctaatg tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg
      301 tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc
      361 gcttcatctt ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt
      421 atcttttgtc tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt
      481 gccatatgta cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa
      541 ctgactggga cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca
      601 ttgactatca cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta
      661 ggacgagttt gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt
      721 acggatacta ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa
      781 ttgatttgct tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat
      841 cttgataaaa cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa
      901 gcatctgcta attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct
      961 tttaaagcgg caagtacttg cttagattgc actagatttt tataggtgta gacattttca
     1021 gcttcaattt gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca
     1081 taggttatgg tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga
     1141 tcaagttcag ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc
     1201 atgttatggg caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca
     1261 tttgaaacat agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc
     1321 ggttgaagtt ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc
     1381 acgattttca tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa
     1441 attggttcaa ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag
     1501 cggacttctg tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt
     1561 ctttctcgta ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt
     1621 ctttcacgat atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta
     1681 attctaactg cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc
     1741 gggcattttc ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc
     1801 tttcacataa aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt
     1861 cttggccaaa aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca
     1921 ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt
     1981 ctatcgattg tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc
     2041 ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga
     2101 aaagataaaa aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac
     2161 gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa
     2221 gattgctata atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa
     2281 gccatatttc aaatttcaat cgtccttctt ctgtaatagt aaaactaaac tctttttgca
     2341 gcagatctaa aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagataag
     2401 gacgtccaaa ccaaaataat tggaaataaa aataacaaat ttgactttct ggaccggtca
     2461 gccgcccgct ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc
     2521 tgcgagacaa agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg
     2581 agccattttc caacagataa agaagtatct gatattttgt agaatagcgc aagtagcaat
     2641 gataataaac atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt
     2701 ggataacaat ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt
     2761 ctttcaaggt agcccgatca actgataagc gggtaagtac gttattaacg gaaatctctc
     2821 ctttttcctc taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca
     2881 aactgaaaag ttccaagtcc actcctccaa gcatttctga ttttaacagc agaagtttcc
     2941 ttctcatcct tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc
     3001 acttagctgg ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca
     3061 ggttgagcat tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca
     3121 acgagaacct ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc
     3181 tgaatcaatt gttttgatcc agaatgattg aagtataatt ttgttccgct taattcttga
     3241 ccttgatcat tcttaaataa agaagtcaat gtccctgtca gttgccaagg agacgtgctt
     3301 ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc
     3361 ttcaacggtt tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt
     3421 aattcttgtt tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc
     3481 ttagcaccat ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg
     3541 acggattgta cagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta
     3601 tctcctattt tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct
     3661 gcgatagttg taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt
     3721 gagatcgtga tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca
     3781 ttttcttgaa tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg
     3841 ctaaggtttt ttagctgact atctgcactc acagatggaa aattagccac tgttatttga
     3901 ggaagagtgg tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg
     3961 ttagtaagta catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca
     4021 ggtagtgtag aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt
     4081 tgatctgtcc atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc
     4141 gaaccctttt ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa
     4201 aggagctttc cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc
     4261 ttgtactgct ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc
     4321 ccactgtaca cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc
     4381 agtattgctc cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga
     4441 ctaaatggca actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg
     4501 gtagagcctg gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta
     4561 ttatattttc ctgttgaatc acttgttgag atgtaactat tccacgtatt tatctctgca
     4621 cttgcagaaa atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat
     4681 ctacctgcag ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa
     4741 tcataaaaat caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg
     4801 gtcaaaattt tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca
     4861 ttagctgtaa catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata
     4921 atagcaataa tatctgccaa tagagtattt gtatcccacg accccatccc aaaacgcaag
     4981 gtatggcttc ctgctgataa taaactaccg ctagtaacct tgatgggggc accggcaata
     5041 tacaagtagg cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc
     5101 ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt
     5161 acatttgtta gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga
     5221 tcatttgaac catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt
     5281 agttggacaa gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt
     5341 tcatacgtgt atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa
     5401 ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat
     5461 gaaaatttat tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa
     5521 ccacgcaaga agccataatt ctataccaat gatcaaataa agaaaacgga tcttttcttc
     5581 attcgtctcc gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg
     5641 ctgttttgat gcgccattga ctgaaccact tggatgcgga actggactta gatcataaaa
     5701 tgtaatcgta cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag
     5761 caaacttgca ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg
     5821 ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca
     5881 ctagaactgc agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa
     5941 aaacggaatt ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa
     6001 atcgccattc attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt
     6061 ttgctttatc accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa
     6121 attcattaga cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata
     6181 tcaaattgct gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg
     6241 cgttgacctc gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac
     6301 tttcttgtaa aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg
     6361 tagcattttc tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc
     6421 ccttaaagct ttttgctggg attttcactt ctataggtat agttgtttct gcatgtgctg
     6481 atacattgat ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct
     6541 catctaacgt tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg
     6601 gattgacaga gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta
     6661 gtgtctgatt ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt
     6721 ggttctctgg aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga
     6781 ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac
     6841 tttgattcat tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta
     6901 tagtattttc accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac
     6961 gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt
     7021 gatatttgct ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt
     7081 aaatattggt gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc
     7141 agtggattcg ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc
     7201 ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg
     7261 tgcttggcca tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt
     7321 ttttgaagcg tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg
     7381 caattcagga actacatcca atgttaaaga acctgtctgg ccagttgctg gattttccgg
     7441 atctgctggc tctactggtt ttgaaggatc agtagggtta agaggctcaa cggtatttgt
     7501 tggttcagta aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt
     7561 tgccaagccg cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat
     7621 aataaatcac tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc
     7681 tcatgaattc atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca
     7741 ataaaatcgt ttacatgaat attcttcctt ttttattaag tatgaaaaaa tatttcaatt
     7801 ttattttttc gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta
     7861 cggcatcatc tgccgcactc ctattttttt cgtcatttat ttcttaaata aaacgattct
     7921 cacgcaattt tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca
     7981 tttccaattc tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag
     8041 acaatccata aataacgggt ctgccaatag caacaagatc ggcacctgat gcgattgctt
     8101 tgaaaacatg ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta
     8161 ctgcttctgc cacatattgc aacgaatcaa acgcagcagg ccccccatct aattgacggc
     8221 caccatggtt agatacccaa attccaccag caccagattc taatgatcga tagacatctt
     8281 cttctgattg aacacctttc acgtagactg gcaaatcaga atgagcagcg ataaattcta
     8341 catcttttgg actaagtttt tgtttggaag atttatatac agcatccatc gtttggccta
     8401 ctcctgattg ataagcctga acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc
     8461 ggtctgtttc acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc
     8521 cattacgctt ggccacttca agaatatcca aattgattcc attatctttg ctcatataaa
     8581 attgaaacca ttgcggtgct ttctccccgc ctgcttctct tatctcttct aaagtacaag
     8641 aagcgtaaga acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt
     8701 tctccgcctt cacatgagct aatccatgag cagcaactgg tgccatgatg attggtgcag
     8761 tcagcatttc ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct cttaatacat
     8821 gaggaataat caaacgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc
     8881 cagcaccact gctgatgtaa ccataaccac cagctggaat cacttgtgaa gccgcgattt
     8941 ctaaatcttc catattgata aaatctacta ttccttcatt tgtacctgct tgatatgttt
     9001 tttccatctc aatgccttct ctctataaaa tttgaatgat gaacaatatg atagcgttta
     9061 cactttttat attactcttc gacattgata caggcaaata atctgtttac atttcagcca
     9121 ttccagatgt cactactctg gaagaaatga ttgtaataaa aatagataaa aaaccgatgg
     9181 gactttgaca aaagtagtgt caaagtccct ttttttgaat aaacggtgtt caaaagccat
     9241 tttctcggta ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
     9301 cttactactc gaaactaagc gactttttca caacctctcg ataaaacggt gttcaatcag
     9361 ctgaccctcg gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc
     9421 tctctaatac tcaggtcaga acgttgactt cacagtctct tccttaaacg gtgttcaaaa
     9481 acttgcatct gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg
     9541 gctgttctta ttacttgatg cagggcaggt ttttcacgac ctctgattca cggtgtttct
     9601 ttttcatcaa catttttacg aaatactgtg gaagactatt gaaataagtg tggattgttt
     9661 ctttgtgttt caatcgtttc ttgccattct tcatttaatt ttggattacc gttttcttcc
     9721 ttgaactcat aaggtcctaa ttcaaaaatc ccagacagta tttgattcac gatttcatat
     9781 agttcagtgt gatcagacac atgattcttg aagatcatct cattatcacc acgtttgaac
     9841 ctcacattcg ggctctcatg atgaacctct atgcattttt ctcctttttt taactgacgg
     9901 acttctgaaa acatctcata aatatcttct ccagtaaaac cttcagcaag cgccagactg
     9961 tccacaagac cgcctactgt cagttcagag attattgctt gattggaatc taattttaat
    10021 gtatagcttg tcgcttctac tacgccacat gcattgcaac cgccttctga tagttcgctt
    10081 tttctaatca attgaacttc tttcattttc tcttcctcct cttaattttc tacttatgta
    10141 tttttatcac ctgacatagg tgactcctat caacgctatc atgtgctttt ttcctcagaa
    10201 atcctattta tccattccat ctatccctta ttcttctact tttctttctc tagcatataa
    10261 gagatacgca tagtattcaa gcaaggattg tcgatataaa aagaacaaat actgctattt
    10321 tgatttcttt tggctatatt acatataaat aaaatttatc atacctgctc tttttgacta
    10381 tatcatcatc tgacttctat acttcgtttg tattaaaact taggaggaat cattcatgct
    10441 tacagggtta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
    10501 agaacgggca aaatatggaa tcactggtat gcttccaagt actgtccaaa cactggaaca
    10561 acaatctgtt caagcttatg gtcaatactt aagcaaacaa acagatttgg aaaaacgtat
    10621 cttcttaatg aatctattca atacgaatcg gacattgttt tataaattga tgggcgaaca
    10681 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
    10741 caacgaaatt ttcttgaaac cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa
    10801 catcaaagca agtttgaaga acgcggcaga tggacgagac attcgtttga ttgttgtgac
    10861 tgatgcagaa ggaatcttag gtatgggcga ctggggtgtg aacggtgttg acatcgctat
    10921 cggcaaattg atggtctata ctgctgctgc tggtattaat ccagcacaag ttttgcctgt
    10981 atcgatcgac gctggtacta ataatgaaga attattgaat aatcccttat acttaggcaa
    11041 tcgtcatgct cgtgtagaag gcgaaactta ctatgaattc atcgatcaat tcgttcaatc
    11101 agctaccgaa ttattcccag aattgttatt gcattgggaa gattttggac gaggaaatgc
    11161 tgcagcgatc ttagaaaaat acgaagatca aatcactact ttcaacgacg atatccaagg
    11221 tacaggaatt gttgtcctag caggtgtatt aggcggattg aatatatccg gtgaacaatt
    11281 gaaagatcaa ataattttga catttggtgc tggtactgct ggtgtgggta tcgcaaacat
    11341 ccttttagat gagatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
    11401 tcaagtagat aaacaaggtt tattgtttga agatacagaa ggattgactc ctggtcaaat
    11461 cccattcgct cgcaaacgct cagatttcac taatgctgat gaattgacaa acttagaagc
    11521 agttgtaaaa gcgatccatc cgacaatcat gattggtact tctacccaac ctggagcatt
    11581 cactgaagaa atcgtgaaag aaatggctgc tcatacacca cgtccgatca tcatgccttt
    11641 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttaatagaat ggacggacgg
    11701 aaaagcatta gttggaacag gtatccctgc tgctgatgtt gaatataatg gtgtaactta
    11761 ccaaatcggt caagcaaata atgctttgat gtacccaggt ttaggacttg gtttgattgc
    11821 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
    11881 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
    11941 attttctcaa acgattgctg aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg
    12001 tgaagaaatc acggatatca aagaagcagt tgaatctgct aaatgggttc ctgaatataa
    12061 atctttagaa gattaagatc gttatagtaa aagaacatac agaaactttg tatgacgtct
    12121 aaaaagaaaa aagcgactgc ggcaaaacag cggccagcca actcctgctc ttttgtcgct
    12181 tttctttggc ttttttcata tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
    12241 aacaacaact gcaactgcag ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat
    12301 caccggcgta agtttgccac tttatttgat catggtcctt gtcctgatca ttgcaatggc
    12361 tttagggaaa ttaccgacga atatgattgg accaatcgca gttttggtta ttttcggtaa
    12421 tttatttcat tttatcggga caaaaatccc tatcgtcaaa agttatttgg gtggtggttc
    12481 cgtttttgcg atcttcgctt ctgctgcgat tgctacattt gggatcttac ctgaatatgt
    12541 agtgaaatcc acagaaaact ttgtcagcaa tatgggcttt tgggactttt atattgctgc
    12601 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
    12661 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtggg
    12721 catgcttatc ggaaatggtt ttgcaaattc cgttttatac atctcattac ctgcgatggc
    12781 tggtggtgtt ggagccggag ccgttccttt atcaacgatt tatgcaaatg ttttaggtac
    12841 ggatgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc
    12901 tatcatcggt gcagcattag ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa
    12961 tggtaaatta atggaaaaag gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc
    13021 cattccacag ttaggtgttg gtttgatggt atcattgact ttcttcatct taggacaaat
    13081 tggtaactat tttgttccta aagtccatgc ttacgcattt atgattatta tcgttgtcat
    13141 ctgtaaaatc gccaacctat taccagagta ttatgaagat gcagctatca tgttcaataa
    13201 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttgctgaa
    13261 tctgaatgta ttagcttctg ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt
    13321 tatcgcgatt tcagttgttt caggatttgt cggtcgtttg tttggtttat atccaattga
    13381 atcaactatt actgctggct tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt
    13441 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcgacttgg
    13501 tggtgccatc gttttgatca tctctggttt cttgatgcaa ttactttctt agtcaaaaaa
    13561 acaaaaatac ctgatcatca agtatcggct tggtgatcgg gtattttttt gataaagatt
    13621 tacaggattt tcaaaaatct tttttgcatt ttatcggaat gataaccgat gtgttcataa
    13681 aattggtgtg cttctactct gtattctgca gaattaagcc ttattgtcgt tattcctcgt
    13741 tttttcgctt cttcttctac gccagtcatc aaagcctttc cgatgcctct tttttcagca
    13801 tatttagaca cagcaagtgc cataatattg aacattggat cgctaaaaat cgattcatag
    13861 gactccgctt gtacatagcc agctgctttt ttagaagtag gatcttcata aaccaagaaa
    13921 aaatttttct ctggctcctt cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt
    13981 atatcatacc ctaattgatc cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc
    14041 atttcccgta acatccaaat caccagccaa tttctgattt tttaaaatga atttttttat
    14101 catagtatac tctttttttc atttagaaca atatgtttca gtgattttga cttgcgttag
    14161 tctagattct cctaatgttt gtaagatgac aaaggcattg taacaattga ccaaaagaac
    14221 cacgaggaca attacttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa
    14281 tcttgctccc cacaaacctc caataacagc agcggggata acataaaaca acatagataa
    14341 atcaaagcgc ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa
    14401 gaaaatcgta gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat
    14461 caacaaagaa acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag
    14521 aacccccccc acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat
    14581 gcttggtgta gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct
    14641 gcacatgctt ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta
    14701 ctcctccaac aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc
    14761 cgcttgatac ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt
    14821 aaaaagaaat ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca
    14881 cgcctccacc catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat
    14941 aaatcagacc aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac
    15001 tcttttcttt tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt
    15061 taaggaaatt tagtgctaaa cgaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    15121 aagaacagcc aaacaaaaat agctcctcat aattttcgtt tggttgaact tattaccgca
    15181 gatgcaagct tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtt cagctccgag
    15241 tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac
    15301 cgaggagttg gcaggagaac accgtttaag gaagagactg tgaagtcaac gttttgacct
    15361 gattattaga gaaacaaatg gcaaaatagt tcttcatatt tcgccgtttt tcgagcgaat
    15421 gccgaggggc agctgattga acatcgttta tttggaaaaa gggattgtga caagacgttt
    15481 gtcacaatcc ctttttttat atcttattaa ttcattcttt tgttctattc ttcttcttcc
    15541 gccatgaatg tattgaatac ttcttcaatc atgtcccatt ctgcgtctgt ttcgatttgt
    15601 tctaactcgc cttctgttcc atcttcgttt tcgatgtatg cataagcttg taattctaca
    15661 tcttcatctt ctgaaacacc agcaggatat aaaagaacat agttacgtcc aaattcttct
    15721 tgtccgtcaa ctgttagtag gatttcatat aatgtttcat ttccttggtc atccaccaat
    15781 gtgatgtgtt cgtgtccttc atggtcgtgg gaatggttat gttctgtcat aagattacct
    15841 cttttctttt attattaaaa taattgctta cctgagccgt ctaaataatt ttgtaagatc
    15901 atcacagctg ctaatttatc gatgactttt tttcgtttgg ctcttgaagt atttgcctgc
    15961 tctactagca tacgttctgc ctgaacagtg gttaatcgtt catcttgata atcaactggc
    16021 aaggagaata gttctttgat cttttctccg tatgacatgg aagcttctgc ccttggtccg
    16081 atggaattgt tcatattttt aggtaatcca actacaaacc ggtctacttc atattgtgct
    16141 actaattcct tcaaccgatc aaagccaaac tcttcttctg cttcattgat ccgaatgatc
    16201 tctactcctt gagctgtcca gccaaagggg tcactgatag caacacctac tgtcttagaa
    16261 ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg tagtccttga
    16321 caattacttc catgatctca tcgcgttcgt gacggcgaat caaatttcga gcatcacgat
    16381 aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga ttgattggat
    16441 tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc acttcttttt
    16501 tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg attctacacc
    16561 tctttcccgc aaggtaattc cagcttacct tctacaacct tattttactc gaagaaaggg
    16621 aaaactacaa acaaatattc caaacttccg aataaattaa gaaacactat ttgtatgttt
    16681 ttttcctttt ttctgtagct ttatcaaaag tactcttttc ataaagtata cgctatattt
    16741 ctttgaatgt atgtttttac acaatttttt cataatattc ataaaaaatt cgaatagctg
    16801 ctactaattt tttgtttttg ttgcttgtcc tgtctcataa ttgatcgtgt agccatcttg
    16861 aacattgtag ataaagacat tgaattcaat ttttttgtct tcaatgcttt ctgcttccaa
    16921 ttgcactccc ctggctacca gttcattccc ctcaaaatga ggagtgacac ggtagcgtac
    16981 atgatgattt gtttctttga tatatgccgc aatatcattt tcgtgagcaa gcatgtatgg
    17041 agcattcaac gaacgtgtcc ctgtcatcaa gtttttaatg ttgttatttt gaccagtcag
    17101 ctggaaacca attaaatggc tgcgattata taaccattgc ccgtcactta atttcttttg
    17161 tttccaacct gtcgggtcaa tatacaaagg ctctctcttt tcagtaggga acaaatcttt
    17221 tccaagcata gcattggctt gtcctactct gttcaattta tcaatatttg aatagctttc
    17281 ccatgttcct ttggaaaggc taagatcctc ttttgtaaat tccggctgat tcccattgat
    17341 ttctatttct tgatgttttc catcatattc tggcacatcc gctacttgag aagcttgtgg
    17401 tgtggaatca aaaatagttg taatctgctg aaagatatct gaatcttttt gtgtggtgga
    17461 aacaccgaat gcccctgcaa tcacagcagc gacgatcccc acgatcgcct tggttgtttt
    17521 tttgctcatt tctttactcc tttactaaca gctccctaaa tcttaacata tgttccccta
    17581 taaataaata ctagaaagaa atagaaacag caaaaaataa aacctgtttt aagatggctt
    17641 aaaaatacaa aaaagagacg gattcctcgt ctctaatcag tcaccactct atctgtaagg
    17701 tatttattat tattcaaaaa ttttcttcat actcaagaca atacactgat ttcacaaccg
    17761 cttggtaaac ggtgcgcaaa agcttgcatc tgcggtaata agtccatcca agttaaaaac
    17821 ataagaagtt gtttttgctt ggctgttctt attacttgtt gcagagcagc tttttcacaa
    17881 cctattttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa gctgggattt
    17941 taccactaaa ctacacccgc aatacaaaaa cagtatacgc ctcttttatt ccttcctcaa
    18001 gtttttttat tctttacgct tctggtatgt tttgtagtaa aataaaacta atcaatcaat
    18061 tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca cttgtttact
    18121 cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag atacgatgag
    18181 tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc aaaccggtct
    18241 ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca accgttcgat
    18301 taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg cttcagagct
    18361 aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt ctttctttcc
    18421 tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg tgttatttgg
    18481 cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat acgcagctta
    18541 tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat cttctttatt
    18601 gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc aggatctaat
    18661 cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg ctatccgtga
    18721 tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga tgatgactgc
    18781 tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg aaaggaacac
    18841 aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg cggcatttgc
    18901 cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg gtgcttttgg
    18961 ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg gttcattgct
    19021 tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga aaatgcccgt
    19081 aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag cttatcaagc
    19141 ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg tccaagcaat
    19201 tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta accataacat
    19261 ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac ggctttttaa
    19321 ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg agcgatgaca
    19381 aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta attatgagta
    19441 ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg
    19501 agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc
    19561 acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat
    19621 agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa
    19681 aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc
    19741 atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc
    19801 cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag
    19861 ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt
    19921 ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac
    19981 ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct
    20041 tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat
    20101 gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt
    20161 tcgcctccta gggcaaaagc aagaataaaa atgattggag ttaacaagga agaagaaaga
    20221 acttgcataa tcaaattagg ttcttttact aattgagcat tgtaactaaa taaaagctga
    20281 tgcacatttt ggttcttttt atgggttctt tttattttga actcactgtt tgaatcagtc
    20341 agttgctctg taagctttgg tactatcagc tgttttacag caaggaaaaa tcccagagcc
    20401 gtgatagcta gtattcccca acccaataaa ccttgttgag aaagtggctg actggcagca
    20461 tcatagaatg gcaataatat cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc
    20521 cccgtttgat gattcatcca catgattcca gcaaatgcga tcagcatcga gctgccaagc
    20581 aagatactag aaactgtttt tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa
    20641 acaatcaaac tagccagaca aaaaagaata atcaaaaata gagaaaacaa aaggattgag
    20701 ataatgatag taagcaaaat ccctagatca gctcgccatc cggttaaaat aaacatgact
    20761 agaagcggaa aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa
    20821 acctgttctt gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag
    20881 atattataaa tgactgatat actttgagtt aaacctaaca taccaaaaag tgccacgtaa
    20941 taagtaaaga aacctggcaa tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc
    21001 agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa gatagatagt taatcgcttt
    21061 cccttttttc ctttctctct tgctttattc gtgagctgtg gattaacata taaaagatta
    21121 actcgaatta attctaataa ttgtcgctta tacatacggt ccatctcctg tcgcttgcct
    21181 tccagccatt ttcaaataaa tcttttctag cgtttcatca ggtgagccat tcaataattc
    21241 actaactgcc ccctgataaa tcaattcacc tttcttcaaa atagccagtt tatcgcataa
    21301 ctgttgtgcg gtatccaata catgtgtaga aaaaatgact gtttttcctt ttgcggcatg
    21361 agccttcatc atttctttca aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc
    21421 atccaatacc caaatatctg gatctggcag caacgtgccg atcaaaatcg ttttttgacg
    21481 cattccatgt gaaaaacttg cgattgtttc attttgatga ctatacatat caaaaagcgc
    21541 tgtcaattct gctaaacgct tttctttttt ttgtgtgtct aattcatagg cagctgaaat
    21601 caagtcccaa tattctccag ccgtcaattg taaaaaaata tctggggaat caggcacata
    21661 accgattttt tgtttgatac tcaatcgatt ttccgtcaat ttcattccat ctactaaaat
    21721 cgttcccgat gatggttcaa taatactaac taaactttta atcgtagttg attttccggc
    21781 accattatgt cctaaaaatc caaaaatttc cccttgtctt atcgagagag acaagtcttt
    21841 taatgcttgt tttttcccgt aacttttact tacattgatg aattcaatca tttctgtact
    21901 ccttttgttt gttattactt tttattgtac ccttcgttat tggtggagta aatgtcaaaa
    21961 agagtgcata acaaaaaagg atagaaacga ctaggctgct acttgggttg ttctcttcta
    22021 caggaaaaca ctcttctaac aggtaagata tgagtctttg ttttctttaa aatgattcat
    22081 tttctatctt ttcttgcata aaattcttta gtttatcaat acttctcttt cccatctcta
    22141 gttgttctcc atttgcaaat gttactattt cgtttactct gctaatagat gtaatgtttt
    22201 cagtattaat gatataagct tttaaatctt tacataaata ctcatattcg agtgattttt
    22261 tatatttttt aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt
    22321 tatttttata acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat
    22381 cgctattacc ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt
    22441 tttctttttc tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt
    22501 cttttatcaa ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg
    22561 gatgagacgt tagataaaca attttacact tttcattctt ttctctaagc atttcgccta
    22621 gttcgatacc gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat
    22681 agatttcatt atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct
    22741 caaatttcca aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt
    22801 caaaaaccaa actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc
    22861 gatattttac tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt
    22921 gtaattctct tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat
    22981 gctgcttcta aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc
    23041 gacgtccttg cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa
    23101 cgatacacat actgattttt cggagcagag actttctcag cgtttgtaac aactacagat
    23161 gggatttcac gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac
    23221 cattcatcat ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct
    23281 gaatctgcca cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg
    23341 ttcggtgttt ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg
    23401 taattataaa ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc
    23461 ttatccccat acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa
    23521 taaatactgg tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct
    23581 ttacttactg ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt
    23641 ggaaacaacg tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga
    23701 taaggttttg acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg
    23761 tttttgaccc acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga
    23821 atagctaagt agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag
    23881 tacatcatca attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta
    23941 aataaataat gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa
    24001 gcgtcctgta aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa
    24061 gaccaaaaag gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa
    24121 cgcatctgct tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc
    24181 taaaatcttt gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc
    24241 agctgcgatt aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga
    24301 atgtgtcaac acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact
    24361 ttgttcttct tcatggacat gatgatggtg attttctgct actttagacg tgattttttc
    24421 tttcaataca gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa
    24481 aaagctgcct atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt
    24541 ggaaaaaatc acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg
    24601 aactggtacg ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga
    24661 aggaaagaaa aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag
    24721 ccatctcttc acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc
    24781 taataatacg aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa
    24841 aaccgaattt ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc
    24901 tttgcaaata tacgctttta ttcaaaaaaa aacaagttgc tctgtaaatt tttacacaac
    24961 tcttattttt taccaaagca cttcaattga ctttttctgt tattcccatt aaattaaggt
    25021 ctcatacata tcacttctat cataaaaggg agaataaaaa tgaaacaaac cactaacttg
    25081 actgaagttc aagacatact gcaacagtca tccgttgccg tcatttatct ttcgatgcct
    25141 aattgcagtg tttgtcacgc tgttcgtcca cgactagagg aacttctcac acattatgat
    25201 attccggcac ttcatttgga tgcatttgaa acaccagaag tagctagtaa atttgaagtg
    25261 ctgactgcgc cagttgtcct gatttttcat caaggaaaag aagtctttcg gcaagcacgg
    25321 ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac tgacagcaga agatgattcc
    25381 ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt ccaaacagaa aaaagcgatt
    25441 gaacaagtga aagttttgcc ttcatttgtt caatcgcttt ttatttttat tctgctgtat
    25501 tttcggaatg cgcatttgcg tcagaatgca agaaatagat caaggtttgc aattcattcg
    25561 tcaaatcaac attttggatc aatacatctg acggagcatt caatcgagca gcggtaaagt
    25621 tcaagattcc tttgattcct gcttccgtta actgattgac tacttcttgt gctctttcag
    25681 cgggtatcgt taaaattgct acttcgattt gttggatctt gatttgttct gacatatctt
    25741 ccattggata aacggggata ccgtctacga tgcgcccaac gatttcctca ttcacatcaa
    25801 aagcacaact gatacgtatg ctattacttt gatggaattt gtattttagt aaagcacttc
    25861 ccaagttccc tacaccgatc agcgcaacat ttgtcaattg atcgtcgttt aatgtctttg
    25921 caaaaaagtg cataagatct tctacatcat aaccataccc acgttttcct aattcgccaa
    25981 aataagaaaa atctcgacgg atcgttgcac tatctacttg aacggcttca cttaattcag
    26041 ttgaagacac ttttttcttt ccagcattat ttaaaatcct taaatagcga taatacaaag
    26101 gcagtctttt agctgtcgcc tttggaataa tatgatcttt cacgaaatga cctccacgta
    26161 ttgtctaatt acatctttga tttctccatc atagcagtat ttataggaaa aaatcaattc
    26221 cattgctcag atattgtgaa attttattct ttattcattt atttatcatt tctgcaaatc
    26281 tcgcagtata atggaattta tggtagacta ctaattgaaa aatagttttt tatttgagga
    26341 gaattcacaa agtagaaaag aggattccat gatattatta caagccaatc agattgctcg
    26401 ccattttggt gcagaagtct tatttgaaaa catacattta gaaatcagta caggcgctcg
    26461 tgtcgcttta gttggcagaa atggcgctgg aaaatcgact ttattaaaga tcattgctgg
    26521 aattgaagca cctgatgaag gaaagatcgc taaaaataaa acagcgactc tcggatacct
    26581 cgctcaggac acaggacttg attccaatga aacagtttgg aacgagatgc tgaaagcttt
    26641 tgaacaagtt agacaaatgg aagagcggat gcgcgagcta gagattttca ttagcgaagg
    26701 tacgccagac acacctgctt atagttctta tttaaaagaa tacgaccggc tgcaacacga
    26761 tttttcagat cgaaacggct atggttatga aaatgaaatt cgttccgtat tgcatgggtt
    26821 taaattcgat tcttcatttt atgatcagaa aatcgatact ttatcaggcg gacagcgcac
    26881 acgtttagct ttagctcgca tgcttttgca acaccctgac attttgattc tagatgaacc
    26941 aacgaaccat ttagatatcg agacactttc ctggcttgag aattatctgc aaagctatac
    27001 tggtgcatta ttgattgttt cccatgaccg ttatttttta gataaagtag tcaatgaagt
    27061 ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt aactattcga aatatctaga
    27121 tttgaaagca gaacagttag caagtgaatg gaaggcttat gaaaagcagc aaacagaaat
    27181 ccataaatta gaagacttcg ttgctcggaa ccttgttcgc gcctctacca caaaacgagc
    27241 gcaaagccgc agaaagacat tagaaaaaat ggaccggctg gatcgtcctc aaggggatga
    27301 aaaacaagca aactttctat tcagtataga aaagccttct ggaaatgtag tcttgcaaat
    27361 cgaagatgcc gctatcggat atgataatgt caccttatct gaaccaatca atctagatat
    27421 ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt atcgggaaat cgacgttgct
    27481 gaaatcaatc attcaccaga tcccttttat ccgtggaaaa gaaacgctag ggacaaatgt
    27541 caccatcggc tattacgatc aaggtcaagc tgatctgcat acaaacaaga cgatcctagc
    27601 agaattgtgg gatgagcatc ctaccactcc agaaaaagag atccgaagtg ctttaggtgc
    27661 tttcttattt tctggcgaag atgtagaaaa accaatccct ctgctaagtg gcggtgaaaa
    27721 agcacgggtt gctttagcca agttagcgat gaacaaagaa aattttttga ttttggatga
    27781 accaacaaac catttagata ttgacaacaa agaagtatta gaaaatgcat tgatcgatta
    27841 tgacggcaca ctgctttttg tttcccatga ccgatatttc atcaatcgga tcgcgacaaa
    27901 agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta ggagactatg attattattt
    27961 agagaaaaag caggaagaag aagaaatcgc cgaattgttg gcacaagatg agccagaatc
    28021 tgctcctgct cccacgaaca aatctgatta ttaccaaagc aaagaacagc aaaagcttat
    28081 ccgtagcctt caacgaaaaa ttactcaggt tgaagaagaa atggcgcgga tcgatgaatg
    28141 gatcgatcag ttggaacaag aaatggtcgc gccagaaaac ctggaagatc acgtaaaact
    28201 caatgagctg aaccaagatt tagaagctgc tagacaagat caagaaacaa aattgacgga
    28261 atgggaagaa ctaagcttgg aattagaaga atatgaaaat taacgaaaaa accaaaacag
    28321 aaaccattca tgctgttttg gtttttcttt ttttatttta tcaaacaaaa attccatatt
    28381 tacctttttc accaataaga agaccaatta ctagtcattc tcactaaagc ttctaaataa
    28441 tagtaatcac cccaaataca aggtgtattt accccattat ttttgggctt attataaaca
    28501 gactgtgtta aaaagccatc ttcttcttcc gttgtagcat atctttcttt caattgtcgc
    28561 ataattttcc aaacagcaga ctggtataag ttcttttctg ttttgtctaa tagacctgat
    28621 tctattaatt ctaataatcc acaaaccatg atcaccatac tagatgtatc tttttcttct
    28681 ctgctagaat catcaaagat caaatcccaa taacaaatgg tatcttctgg caaccgattc
    28741 ataaaataat tggctaatat tttgcttaaa tcaaaatatt tttgttcttt caaataacga
    28801 taacttaatg caaaaccgta aatcccccat gcttggcctc tagcccaaca ggaattatca
    28861 ctataacctt gatgcgtact tccatatctt ggttttccat ctttgacatc catataaaat
    28921 gtatgataag ttgaaccatc ttctcttact aaataatctg ctgcattttg aatatgagcc
    28981 tgagcagctt ttttatattt ttgatttcca gtttcttcac tcgcccaata caataatgga
    29041 agattcatgc aacaatccat gatcattcta ccttgttgtg aaggatcact aggatctccc
    29101 caagcttgta taactcctgc cttctcccaa aaacgctgaa gtaatgtttc tgcagcatca
    29161 attgaagctt ccttcatttt tcgatttccc gttattttat aatctgctac ggttgataaa
    29221 gaatacaaaa aaccaagatc gtgatgatta gtgataattt gacagtcaat cctgtggata
    29281 aaatctatat ctgctttttc agctgtttgt tgaaataaag gatcacccgt cttctcaaat
    29341 aacaaccaat taattcctgt ccaaaatcct tctgtccaac caatattgtc tcttaatgga
    29401 taacgtcctt ttataggaca ctcgcttggg aattgatact gactgttact gatacgtttg
    29461 attaacctct ccgtgtgtct gcgacaacgc tcgatctcat tattccattc tttttccatt
    29521 aattctttat aacattcatt tatccatttt tctggatgta ttatctcttc aaacatcact
    29581 ttatcaagca tttccccacc agcctttagc ataattttag tcttttttta ttgtttttat
    29641 tttggatagt tagcccggtt gtcccatcaa aatcaatttt gggacgattg ttccatactt
    29701 ctaaactata atcccgatct tgatgagcaa ccactcctgt tccgagccaa tgggttcctt
    29761 ttccaaatgt accctctaat ccaggaataa aagtgttttc ccatgtcatc aagtttgtat
    29821 tgggcaaacc cataatagct gtggattttc gtttaatagt atcgtcactt aaacagataa
    29881 tacctgaaaa gccagcttca ctcaccctat agttttcctt gtcttcttct atgattattc
    29941 cttctttctc aggagaacat tcaatagcaa accctgtctc atatagaaca tattctgtat
    30001 ctaattctac tttgtgtacg cgaatctgcc agccttcaaa aggaataagc caagttctga
    30061 tttttgcctg taaagtaggt tcccatgtag aggcaagtac ctctttctta catacaatgt
    30121 ctcgtacatt atttcgaacg atgaactgtc cataggtttt gatgctctca ttcttaaaac
    30181 tggtcgtgat atatgtgtcc gctcgctgaa tccctagcat tgaatccatt gccatttctg
    30241 ctttcaattg attcgctcgc ggtatcgaaa aaccaaattg actagaataa gcaaatttta
    30301 aatatttttc attggtcaat gtatggaaat ttggccctaa ttgtcccgca ttcaaaaaaa
    30361 atgtatgtcc attatcatgt tgtatgagca aattggcttc tgaaagaagt ttagttcctt
    30421 cacgaatagg cataggcatt tctttagccg tccaatactc atggtcttca ggtagcgcca
    30481 acaataaaaa aatcttattc agccataatg gagataacgt actattatag ggttcagtca
    30541 atgtcaattg agggtaggta taaccaatat tcaggatacc tttttcatca aatatttctt
    30601 tttgcatcca ccatcttata ttccgattga ctaacccctt gatttcacct aatttaaaag
    30661 gcaaaagatc ggcccacaac atagctgacc aaaagcttga aactgcaaaa cgataaatca
    30721 tgcttcgacc aaaaggcaca tttgcaccat cttccgcaaa aaaatgaata tagtctctag
    30781 aaaaatcaat agctcggttc ttatatatct ggctaatggg cttttctggt tctagtacgc
    30841 tataaatcaa accataataa tgaaatgcaa aaggattata ataatcttgg cgacctcttg
    30901 aactgtctcg ataccagcca ttttgaagat aacaattttc tatattatca aatgcttctt
    30961 tcagtgctga ttctttaact ggataaccaa atttttggat aattgttccg acaattatct
    31021 taaaaaattg ccaattacca tctgcacatt catattcaaa aatttgcgag aaccaatgaa
    31081 taatctggct tttttctcct gtatctaatt ttttccataa taattgttca tgatgatata
    31141 acgctaataa cattgcgggc atttctacca tacgctggtc tctttcatgt atttctcccc
    31201 aataatcggg atgggaagga tctgttccag cttttattcc tgatagaatt aatttcaacc
    31261 atcgttcatc taattggtca attgcgggcc ccgctcccca taaaacccga gagaatcctt
    31321 ccattcctgc aattttattt ccatatccta ccccttgatg tatataatca atttgtgcgc
    31381 cacttgctcg ataaaacggc tggagtggtt gatttatctt tttaaataag tcaaagtaat
    31441 cctgtctggt ccttaatagt ggtactggat gtttcattgt attttctcct ctttttacta
    31501 ccaaatagct aattccgact gttctaatcg aagtaagcct tcaataaaaa aataatcaga
    31561 atagatcagt ggcacattat aagtcagttc tctatggtat gcttctgctc cgcctccaat
    31621 gatcccatct tcatccatat tccaatttgc atagtgcttt tctgtctttt ggagtatatt
    31681 gatagcagct tcatgataca tttttgatcg tacagaggaa acatatcctg ctaattccag
    31741 aagaccgcat gcagcgcata ctccagcact ggtatcaata ttcgaatctt ttacagcaaa
    31801 atcaatgaga ggaatataac cagttttttc gattttctga ataaaattta ctgctatctt
    31861 ttctgctata gctagatagt acggattttt tgtgtaacga taacttaacg cgaatccata
    31921 taaagcccat gcatttcctc tgctccaaca agaattctca ttgtatcctt gcccgccaag
    31981 taaccgaata tatttccctg attctgcatc aaattctgcg atatgcccgg ttgatccatc
    32041 tttcctcact agatacttag ctgttgtatc tgcatgctgc cgagcaattt tagagaacct
    32101 cggatcacca gtttccctcg aagcccaata cagtaatggt aaattcatca tactatcaat
    32161 aatcatccaa ccaggtttat ggtcattcca tgctactaaa tagttccctg aaatattata
    32221 gcgtccagca agtaccgtag ctgcatgtaa ccctagttgt cgactatgtt tgttttgaga
    32281 ctgttgataa tgtgcaatag ccgatggtaa gaataaaaaa cctacatcat gatgaacgtc
    32341 tgaaaaatgg tacagggcag attctaataa ttgttcttgt tgtattgctg cctctttgaa
    32401 cttttctcgt tttgtcttgt gaaagagctg ccataaaata ccactccaaa aaccattggt
    32461 ccaccagtcg attcctctgt cctttattac gtctgtatat tttccgttgt aagaaatata
    32521 tggaatttta ttccccattc tgtctaattc cgcatctatc ttttcagaag ctacattcaa
    32581 aatttttctt gaccattcct tcgtctgttc attcatggat atcccgccat tcttttaatt
    32641 tttatgaatg tggagttgct cctagaacga actagatggt taacattcgt tatttttttt
    32701 acttatttgc agctccaatg tcattctcct taaatttaga ctgcgcccac acataaatca
    32761 agaccattgg gatcactgaa gttgctaaaa ttagcataat tgcgccataa ttaattggct
    32821 cacctgtact agaaaccgtc atatttttga taattaaggg aaccgtatat ttatctttat
    32881 ctgaaagcat gactaatgga aatagatatt gattccaact tcccataaag ataagtaaac
    32941 cactagtaat aatcgtaggt gataaaatcg gtacagcgat tttcgtaaaa atcacccact
    33001 cattggcacc atcgattctg gcagcctcca tcagtgctgt aggaaattga ctgctatatt
    33061 gtcgcatcat aaacactgaa gttgtactgg caatcgaagg gatgatcaca cctgcataag
    33121 tattcgataa tcctaaacga ctcataatgt aaaataaagg aatcaatgtg gtaaaaaaag
    33181 gcaccatcct agatagcata actgtattga acaacagttt attccccata aaacgatact
    33241 ttgctaatgc ataacccgcc attgtagaac tggctgtagc taaaactgta ctgattacag
    33301 caataacaat cgaattccaa ataatcaagg gataattata tctcgccatg atggcttgcc
    33361 agttttttaa tagcatatcc ccaaacttga aattgattcc gccacttaaa atttcttgat
    33421 tactttgcgt tgaagctaca atcatatata aatatggcat caaaaatatt aaggcaccaa
    33481 acacaataat gacgtacgta aaaattctgc tttgtatctt ttgtttagtc atttttatca
    33541 cctccgaatt taaattgaat agccgataat ataacagata caaacaccat cgtccaggca
    33601 atagcagagg cttttccaaa atcaaccatt tcaaaacttg ttttatataa attgactcct
    33661 acgaccaaag ctgaattttc tggtccattg gaagtcccaa agaatatatt tgggatattg
    33721 aaattaccca agccattgat agtggctaaa actgtggcca ttaaaatgat tggctttagc
    33781 atggggatcg ttaataaaaa gaattgccga aatttcgatg ctccatctat ttcagcactt
    33841 tcgtatatct ctttagaaat cccttgaaga ccagctaaaa ataaaatagt gttatatcct
    33901 acactaagcc aaatcatcgt gatgatcatt gatattcgta tccaaaaagg atttgttaac
    33961 cactgtacac cgttaattcc tagcgcattc aaaaatccat tgaataaccc agtcggattg
    34021 aaaaaagtca tgaatacgga agctacagcc accgttgatg taatggttgg aatataatag
    34081 acagttctaa aaaatccttt tccttttaac tgtgctgaat tcaataaaac agcaaagtaa
    34141 atagctaata gcatcgttat tggaattgat cctgccatga taatcaaaac attcgtgtaa
    34201 gatttaataa acaatggatc tcggaagata gctagataat tgtcaaatcc aacaaattca
    34261 aatgagtagc caattttcat aaaactaaaa gcaaacgaga taatgatggg aatcaaaatc
    34321 gtcacaataa agaaaataac aaaaggtgtg accaatccat aagcactttt tttatgagac
    34381 gtatctgttg cttttttctt gctgttcttt attgtcttgc ttggtaacga agattgcatc
    34441 atcgttaagt cctcctaata ttttttaatc accagttaat tttacgccgt acttatgagc
    34501 aaaattttct gcttctttta gatatgaatt ttcaaaatca cctgtttgcc aatagttata
    34561 tgcagcggta gtcaaataag acgtagcatc tgaataagtg gctggtaaaa cagtatttga
    34621 tgtattttca ttgattttat tcaatagttc atagtatttt tgtcctcctt agtagtcaaa
    34681 ctttttctca gctgcttctg tttgataggc caatgagtta gatgtagcca cgcccagctc
    34741 cagtgcgttg gctaagcatt cttcatctgt cagcataaat gtaagaaatt gttgtgccac
    34801 ttgtggattt ttggcatctt tagggacata aaaacttgat cctcctgata ctgcaccttt
    34861 tcctggattt tcttttgaaa aaggaatcag tggagccatg cgccatttgt cagatgcatc
    34921 aggaaaattc aaagacatat tcgtagcata ccagccgcca gcaacaaaca ttgcagattc
    34981 ttgaaatgct ttctcagcat cttgtgcacc gtagaaatgt acaatatctg agtcgatcat
    35041 ttctttgatt aactttgcag catgtttcgc ttctttagta ttcagacgga tatttccttc
    35101 ttcatcaagc aaagattgat tctgctgggc tagcatgttg ataaacaatt cttggtcccc
    35161 agattctggc aaggcaattc caaaactacc agttttttct tttaatgctt ttgccacctg
    35221 gatatattct tcccaagaac tgatttcttc aaaatctaca cctatttcag caaatttttt
    35281 tggttgataa taacaaagtg ctggggtaaa atcactagcc caaggaataa tcgtttcatt
    35341 actcgtttgt tgacgcaaaa tattcaattt ttgatcataa aactgatcgc catatttgtc
    35401 ataaaatcca tattcatcag cactataaaa agcatcttga aatttttcca gcacacctgc
    35461 tgctgaagtg tctgccatgg agataatatc cggcatatct tcgtgtgtta ccaattttgg
    35521 ggtgatcatt tgtactgttg ctgttcccga agctacattc acaattttta agtcaatatt
    35581 tgctccagtt ttgcggttat agatttttgc agcattttca acatagtttg tcagtttgcc
    35641 gtttgcgaca tatacttcta atgtagtggc tttttttatc attggatctg cgatgtttgc
    35701 tgttacttct tcgttagttt ttccacatgc acttaagctg aataataggg taatgtttag
    35761 caaagctatc accaatttgt atcttttttt catgtctatc ccttctttct tttcaagttc
    35821 ttatataaaa cgctttcact tatgtatatt aacggaatta gtaacacaaa tatttatata
    35881 aaaaaatatt ttacttgtcg ttttggcttt tcatttttgt tataatagtc attaattata
    35941 aatagaaagc tttttatata aaaagagaaa ggaaagtggt tcttttgtta acaaacaacc
    36001 aatcctccaa tcttgtcaaa aaaacactaa cctattccat agatacccat cttcatttat
    36061 ctgctgctcc ctattttcga ctactaataa atgaaagcgc taatttatat gctgaatttg
    36121 cacataaatc tatagaacta gataatcctt cccttttatt cattgataaa cagcaagaag
    36181 tccaaatctt ttctactcaa aaggaaacac aaacttttat cattatccag ttttattttg
    36241 atgagcaatt attagcacat attacgggac aatctttttc tatagatcaa gaaataacat
    36301 ctatcattac ttttctgaaa aatgaacagt tcaaaattga ttatctaaaa catagtaagc
    36361 cagctatttc ctatgatcag gaaatcaatt cttttttcat actatgccaa cagatgagca
    36421 ttcatttgct ttccttatta gttacttatt gtctgcaaaa aattcaattg tcttccttac
    36481 caaggcatta tcaacaggaa cttgctttat tgacaatacc ttcaaatgag aaagtcattc
    36541 ctaaaaaaaa gcatattctt tattcagatg aattagttga tccaatcata cgatatttag
    36601 aagaacatct ttcagaaaat tttttgcttg aacaattagc ccaaattttt tttatcagtc
    36661 cagccagctt aaaaaaacat tttagaaaag tgaatggaaa aagtgttatt agctatttta
    36721 aagaactaaa aatggaaaaa gcaaaacgtc tcgtgctaga aaatgaatta acctatacag
    36781 aaattgcaga tatattagga tttcattcga tccatcattt ttcttcagct tttaaaaaat
    36841 atacagggtt at
//
LOCUS       DAPCMU010000013        35015 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPCMU010000013 DAPCMU010000000
VERSION     DAPCMU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.013"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            164..1309
                     /locus_tag="RD744_000530"
     CDS             164..1309
                     /locus_tag="RD744_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDU0556559.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="RD744_000531"
     CDS             1446..1790
                     /locus_tag="RD744_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDU0556560.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="RD744_000532"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="RD744_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDU0556561.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="RD744_000533"
     CDS             complement(3485..4084)
                     /locus_tag="RD744_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDU0556562.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="RD744_000534"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="RD744_000534"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDU0556563.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="RD744_000535"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="RD744_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDU0556564.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="RD744_000536"
     CDS             7290..8408
                     /locus_tag="RD744_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDU0556565.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="RD744_000537"
     CDS             complement(8468..9760)
                     /locus_tag="RD744_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDU0556566.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="RD744_000538"
     CDS             9892..10470
                     /locus_tag="RD744_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0556567.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="RD744_000539"
     CDS             10599..10976
                     /locus_tag="RD744_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDU0556568.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="RD744_000540"
     CDS             10909..12981
                     /locus_tag="RD744_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDU0556569.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="RD744_000541"
     CDS             complement(13192..14139)
                     /locus_tag="RD744_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0556570.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="RD744_000542"
     CDS             14385..14849
                     /locus_tag="RD744_000542"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDU0556571.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="RD744_000543"
     CDS             complement(14911..15954)
                     /locus_tag="RD744_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDU0556572.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="RD744_000544"
     CDS             complement(16331..17296)
                     /locus_tag="RD744_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDU0556573.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="RD744_000545"
     CDS             17530..18375
                     /locus_tag="RD744_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0556574.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="RD744_000546"
     CDS             18566..19753
                     /locus_tag="RD744_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDU0556575.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="RD744_000547"
     CDS             19759..20115
                     /locus_tag="RD744_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDU0556576.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="RD744_000548"
     CDS             20117..20455
                     /locus_tag="RD744_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDU0556577.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    20531..20787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20850..21884
                     /locus_tag="RD744_000549"
     CDS             20850..21884
                     /locus_tag="RD744_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0556578.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="RD744_000550"
     CDS             21877..22563
                     /locus_tag="RD744_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556579.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="RD744_000551"
     CDS             22587..23435
                     /locus_tag="RD744_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDU0556580.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="RD744_000552"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="RD744_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDU0556581.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="RD744_000553"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="RD744_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDU0556582.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="RD744_000554"
     CDS             25701..26936
                     /locus_tag="RD744_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDU0556583.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="RD744_000555"
     CDS             26923..27393
                     /locus_tag="RD744_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDU0556584.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="RD744_000556"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="RD744_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDU0556585.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="RD744_000557"
     CDS             complement(28884..30026)
                     /locus_tag="RD744_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0556586.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="RD744_000558"
     CDS             complement(30065..30799)
                     /locus_tag="RD744_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0556587.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="RD744_000559"
     CDS             31031..31306
                     /locus_tag="RD744_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556588.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="RD744_000560"
     CDS             31404..33401
                     /locus_tag="RD744_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556589.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="RD744_000561"
     CDS             complement(33477..33812)
                     /locus_tag="RD744_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0556590.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="RD744_000562"
     CDS             33939..34154
                     /locus_tag="RD744_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0556591.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="RD744_000563"
     CDS             34191..34553
                     /locus_tag="RD744_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDU0556592.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="RD744_000564"
     CDS             34599..34925
                     /locus_tag="RD744_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556593.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11526 a         6214 c         7340 g         9935 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ctataaatta aatac
//
LOCUS       DAPCMU010000014        34801 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPCMU010000014 DAPCMU010000000
VERSION     DAPCMU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.014"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            157..1863
                     /gene="efbA"
                     /locus_tag="RD744_000565"
     CDS             157..1863
                     /gene="efbA"
                     /locus_tag="RD744_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDU0556594.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="RD744_000566"
     CDS             complement(2021..2923)
                     /locus_tag="RD744_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0556595.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3612)
                     /locus_tag="RD744_000567"
     CDS             complement(2920..3612)
                     /locus_tag="RD744_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDU0556596.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="RD744_000568"
     CDS             complement(3655..4296)
                     /locus_tag="RD744_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0556597.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4334..4603)
                     /locus_tag="RD744_000569"
     CDS             complement(4334..4603)
                     /locus_tag="RD744_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556598.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4576..5265)
                     /locus_tag="RD744_000570"
     CDS             complement(4576..5265)
                     /locus_tag="RD744_000570"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDU0556599.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="RD744_000571"
     CDS             complement(5272..5988)
                     /gene="pyrF"
                     /locus_tag="RD744_000571"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDU0556600.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5985..6917)
                     /locus_tag="RD744_000572"
     CDS             complement(5985..6917)
                     /locus_tag="RD744_000572"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDU0556601.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6914..7690)
                     /locus_tag="RD744_000573"
     CDS             complement(6914..7690)
                     /locus_tag="RD744_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDU0556602.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7744..10929)
                     /gene="carB"
                     /locus_tag="RD744_000574"
     CDS             complement(7744..10929)
                     /gene="carB"
                     /locus_tag="RD744_000574"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDU0556603.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10934..12010)
                     /gene="carA"
                     /locus_tag="RD744_000575"
     CDS             complement(10934..12010)
                     /gene="carA"
                     /locus_tag="RD744_000575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDU0556604.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12015..13295)
                     /locus_tag="RD744_000576"
     CDS             complement(12015..13295)
                     /locus_tag="RD744_000576"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDU0556605.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13314..14240)
                     /locus_tag="RD744_000577"
     CDS             complement(13314..14240)
                     /locus_tag="RD744_000577"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDU0556606.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14253..15536)
                     /locus_tag="RD744_000578"
     CDS             complement(14253..15536)
                     /locus_tag="RD744_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDU0556607.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="RD744_000579"
     CDS             complement(15550..16089)
                     /gene="pyrR"
                     /locus_tag="RD744_000579"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDU0556608.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16324..17235)
                     /locus_tag="RD744_000580"
     CDS             complement(16324..17235)
                     /locus_tag="RD744_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDU0556609.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17232..17696)
                     /locus_tag="RD744_000581"
     CDS             complement(17232..17696)
                     /locus_tag="RD744_000581"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDU0556610.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17701..18399)
                     /locus_tag="RD744_000582"
     CDS             complement(17701..18399)
                     /locus_tag="RD744_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0556611.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18386..18772)
                     /locus_tag="RD744_000583"
     CDS             complement(18386..18772)
                     /locus_tag="RD744_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556612.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18919..20589)
                     /locus_tag="RD744_000584"
     CDS             complement(18919..20589)
                     /locus_tag="RD744_000584"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDU0556613.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20772..21005
                     /locus_tag="RD744_000585"
     CDS             20772..21005
                     /locus_tag="RD744_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDU0556614.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21104..21535)
                     /locus_tag="RD744_000586"
     CDS             complement(21104..21535)
                     /locus_tag="RD744_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDU0556615.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21614..22027
                     /locus_tag="RD744_000587"
     CDS             21614..22027
                     /locus_tag="RD744_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDU0556616.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22065..23213)
                     /locus_tag="RD744_000588"
     CDS             complement(22065..23213)
                     /locus_tag="RD744_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDU0556617.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23335..23931)
                     /locus_tag="RD744_000589"
     CDS             complement(23335..23931)
                     /locus_tag="RD744_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0556618.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="RD744_000590"
     CDS             complement(24131..26773)
                     /gene="alaS"
                     /locus_tag="RD744_000590"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDU0556619.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27161..27592
                     /locus_tag="RD744_000591"
     CDS             27161..27592
                     /locus_tag="RD744_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0556620.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27637..28986)
                     /locus_tag="RD744_000592"
     CDS             complement(27637..28986)
                     /locus_tag="RD744_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDU0556621.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29056..30219)
                     /locus_tag="RD744_000593"
     CDS             complement(29056..30219)
                     /locus_tag="RD744_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDU0556622.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30348..30671
                     /locus_tag="RD744_000594"
     CDS             30348..30671
                     /locus_tag="RD744_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556623.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30854..32203)
                     /locus_tag="RD744_000595"
     CDS             complement(30854..32203)
                     /locus_tag="RD744_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0556624.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32272..33225)
                     /locus_tag="RD744_000596"
     CDS             complement(32272..33225)
                     /locus_tag="RD744_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDU0556625.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNVLEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33327..34517)
                     /locus_tag="RD744_000597"
     CDS             complement(33327..34517)
                     /locus_tag="RD744_000597"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDU0556626.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9896 a         7616 c         6102 g        11187 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaaaaata gagaaaatct atatataata gaaaaaagag
     4441 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
     4501 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
     4561 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
     4621 cttcatcaag cgcgcttcaa tctgccaatt ttcaggatct tttttccatt cttttagcag
     4681 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
     4741 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
     4801 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
     4861 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
     4921 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
     4981 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
     5041 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
     5101 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
     5161 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
     5221 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
     5281 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
     5341 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
     5401 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     5461 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     5521 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     5581 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     5641 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     5701 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     5761 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     5821 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     5881 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     5941 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     6001 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     6061 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     6121 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     6181 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     6241 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     6301 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     6361 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     6421 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     6481 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     6541 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     6601 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     6661 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     6721 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     6781 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     6841 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     6901 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     6961 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     7021 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     7081 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     7141 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     7201 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     7261 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     7321 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     7381 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     7441 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     7501 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     7561 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     7621 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     7681 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     7741 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     7801 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     7861 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     7921 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     7981 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     8041 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     8101 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     8161 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     8221 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     8281 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     8341 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     8401 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     8461 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     8521 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     8581 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     8641 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     8701 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     8761 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     8821 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     8881 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     8941 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     9001 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     9061 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tatccgaaat
     9121 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     9181 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     9241 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     9301 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     9361 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     9421 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     9481 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     9541 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     9601 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     9661 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     9721 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     9781 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     9841 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     9901 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     9961 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
    10021 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
    10081 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
    10141 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
    10201 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
    10261 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
    10321 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
    10381 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
    10441 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
    10501 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
    10561 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
    10621 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
    10681 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
    10741 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
    10801 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
    10861 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
    10921 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
    10981 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
    11041 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
    11101 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
    11161 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
    11221 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
    11281 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
    11341 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
    11401 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
    11461 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
    11521 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
    11581 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
    11641 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
    11701 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
    11761 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
    11821 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
    11881 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
    11941 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
    12001 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
    12061 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
    12121 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
    12181 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
    12241 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
    12301 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
    12361 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
    12421 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
    12481 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
    12541 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
    12601 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
    12661 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
    12721 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
    12781 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
    12841 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
    12901 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
    12961 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
    13021 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
    13081 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
    13141 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
    13201 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
    13261 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
    13321 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
    13381 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
    13441 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
    13501 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
    13561 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
    13621 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
    13681 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
    13741 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
    13801 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
    13861 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
    13921 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
    13981 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
    14041 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
    14101 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
    14161 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
    14221 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
    14281 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
    14341 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
    14401 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    14461 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    14521 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    14581 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    14641 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    14701 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    14761 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    14821 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    14881 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    14941 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    15001 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    15061 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    15121 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    15181 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    15241 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    15301 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    15361 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    15421 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    15481 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    15541 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    15601 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    15661 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    15721 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    15781 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    15841 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    15901 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    15961 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    16021 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    16081 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    16141 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    16201 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    16261 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    16321 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    16381 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    16441 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    16501 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    16561 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    16621 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    16681 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    16741 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    16801 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    16861 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    16921 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    16981 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    17041 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    17101 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    17161 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    17221 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    17281 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    17341 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    17401 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    17461 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    17521 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    17581 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    17641 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    17701 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    17761 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    17821 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    17881 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    17941 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    18001 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    18061 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    18121 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    18181 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    18241 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    18301 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    18361 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    18421 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    18481 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    18541 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    18601 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    18661 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    18721 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    18781 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    18841 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    18901 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    18961 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    19021 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    19081 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    19141 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    19201 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    19261 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    19321 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    19381 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    19441 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    19501 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    19561 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    19621 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    19681 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    19741 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    19801 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    19861 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    19921 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    19981 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    20041 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    20101 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    20161 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    20221 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    20281 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    20341 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    20401 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    20461 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    20521 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    20581 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    20641 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    20701 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    20761 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    20821 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    20881 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    20941 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    21001 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    21061 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    21121 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    21181 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    21241 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    21301 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    21361 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    21421 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    21481 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    21541 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    21601 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    21661 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    21721 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    21781 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    21841 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    21901 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    21961 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    22021 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    22081 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    22141 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    22201 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    22261 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    22321 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    22381 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    22441 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    22501 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    22561 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    22621 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    22681 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    22741 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    22801 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    22861 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    22921 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    22981 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    23041 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    23101 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    23161 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    23221 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    23281 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    23341 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    23401 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    23461 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    23521 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    23581 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    23641 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    23701 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    23761 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    23821 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    23881 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    23941 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    24001 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    24061 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    24121 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    24181 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    24241 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    24301 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    24361 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    24421 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    24481 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    24541 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    24601 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    24661 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    24721 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    24781 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    24841 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    24901 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    24961 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    25021 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    25081 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    25141 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    25201 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    25261 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    25321 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    25381 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    25441 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    25501 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    25561 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    25621 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    25681 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    25741 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    25801 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    25861 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    25921 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    25981 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    26041 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    26101 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    26161 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    26221 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    26281 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    26341 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    26401 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    26461 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    26521 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    26581 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    26641 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    26701 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    26761 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    26821 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    26881 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    26941 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    27001 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    27061 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    27121 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    27181 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    27241 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    27301 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    27361 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    27421 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    27481 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    27541 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    27601 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    27661 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    27721 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    27781 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    27841 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    27901 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    27961 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    28021 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    28081 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    28141 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    28201 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    28261 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    28321 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    28381 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    28441 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    28501 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    28561 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    28621 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    28681 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    28741 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    28801 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    28861 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    28921 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    28981 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    29041 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    29101 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    29161 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    29221 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    29281 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    29341 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    29401 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    29461 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    29521 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    29581 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    29641 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    29701 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    29761 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    29821 ccggaaataa tatcttaaat aaatgccaaa tggtatgagt aataaaacat taaaagctgg
    29881 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    29941 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    30001 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    30061 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    30121 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    30181 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    30241 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    30301 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    30361 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    30421 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    30481 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    30541 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    30601 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    30661 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    30721 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    30781 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    30841 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    30901 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    30961 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    31021 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    31081 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    31141 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    31201 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    31261 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    31321 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    31381 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    31441 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    31501 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    31561 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    31621 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    31681 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    31741 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    31801 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    31861 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    31921 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    31981 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    32041 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    32101 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    32161 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    32221 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    32281 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    32341 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    32401 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    32461 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    32521 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    32581 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    32641 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    32701 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    32761 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    32821 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    32881 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaata cattgaccag
    32941 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    33001 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    33061 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    33121 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    33181 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    33241 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    33301 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    33361 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    33421 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    33481 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    33541 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    33601 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    33661 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    33721 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    33781 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    33841 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    33901 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    33961 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    34021 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    34081 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    34141 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    34201 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    34261 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    34321 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    34381 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    34441 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    34501 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    34561 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    34621 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    34681 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    34741 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    34801 t
//
LOCUS       DAPCMU010000015        33736 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPCMU010000015 DAPCMU010000000
VERSION     DAPCMU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.015"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            214..498
                     /locus_tag="RD744_000598"
     CDS             214..498
                     /locus_tag="RD744_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDU0556627.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="RD744_000599"
     CDS             complement(779..1198)
                     /locus_tag="RD744_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDU0556628.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="RD744_000600"
     CDS             complement(1200..1784)
                     /locus_tag="RD744_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDU0556629.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="RD744_000601"
     CDS             complement(1831..3306)
                     /locus_tag="RD744_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDU0556630.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="RD744_000602"
     CDS             3487..3798
                     /locus_tag="RD744_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556631.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="RD744_000603"
     CDS             complement(3913..6249)
                     /locus_tag="RD744_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0556632.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="RD744_000604"
     CDS             6391..7059
                     /locus_tag="RD744_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0556633.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="RD744_000605"
     CDS             7062..8105
                     /locus_tag="RD744_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0556634.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="RD744_000606"
     CDS             complement(8342..9223)
                     /locus_tag="RD744_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0556635.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="RD744_000607"
     CDS             9382..10110
                     /locus_tag="RD744_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDU0556636.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="RD744_000608"
     CDS             10266..10583
                     /locus_tag="RD744_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0556637.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="RD744_000609"
     CDS             10576..11184
                     /locus_tag="RD744_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDU0556638.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="RD744_000610"
     CDS             11239..12030
                     /locus_tag="RD744_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDU0556639.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12680
                     /locus_tag="RD744_000611"
     CDS             12168..12680
                     /locus_tag="RD744_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDU0556640.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="RD744_000612"
     CDS             12706..13578
                     /locus_tag="RD744_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDU0556641.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="RD744_000613"
     CDS             13754..14116
                     /locus_tag="RD744_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0556642.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="RD744_000614"
     CDS             14125..14877
                     /locus_tag="RD744_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556643.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="RD744_000615"
     CDS             14891..16693
                     /locus_tag="RD744_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556644.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="RD744_000616"
     CDS             16970..18010
                     /locus_tag="RD744_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDU0556645.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="RD744_000617"
     CDS             complement(18062..20206)
                     /locus_tag="RD744_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDU0556646.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="RD744_000618"
     CDS             complement(20563..21936)
                     /locus_tag="RD744_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0556647.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="RD744_000619"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="RD744_000619"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDU0556648.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="RD744_000620"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="RD744_000620"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDU0556649.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="RD744_000621"
     CDS             complement(23384..24397)
                     /locus_tag="RD744_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0556650.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="RD744_000622"
     CDS             complement(24478..25077)
                     /locus_tag="RD744_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0556651.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="RD744_000623"
     CDS             25263..25964
                     /locus_tag="RD744_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556652.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="RD744_000624"
     CDS             25974..28685
                     /locus_tag="RD744_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556653.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="RD744_000625"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="RD744_000625"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDU0556654.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="RD744_000626"
     CDS             complement(30770..31951)
                     /locus_tag="RD744_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0556655.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="RD744_000627"
     CDS             32203..33726
                     /locus_tag="RD744_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDU0556656.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10690 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaa
//
LOCUS       DAPCMU010000016        31657 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPCMU010000016 DAPCMU010000000
VERSION     DAPCMU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.016"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(243..2135)
                     /locus_tag="RD744_000628"
     CDS             complement(243..2135)
                     /locus_tag="RD744_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556657.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2132..3859)
                     /locus_tag="RD744_000629"
     CDS             complement(2132..3859)
                     /locus_tag="RD744_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556658.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3875..4525)
                     /locus_tag="RD744_000630"
     CDS             complement(3875..4525)
                     /locus_tag="RD744_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDU0556659.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4729..5025
                     /locus_tag="RD744_000631"
     CDS             4729..5025
                     /locus_tag="RD744_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDU0556660.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5197..5817
                     /locus_tag="RD744_000632"
     CDS             5197..5817
                     /locus_tag="RD744_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDU0556661.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5909..7075)
                     /locus_tag="RD744_000633"
     CDS             complement(5909..7075)
                     /locus_tag="RD744_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0556662.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7087..7440)
                     /locus_tag="RD744_000634"
     CDS             complement(7087..7440)
                     /locus_tag="RD744_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDU0556663.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7568..8590
                     /locus_tag="RD744_000635"
     CDS             7568..8590
                     /locus_tag="RD744_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDU0556664.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8609..8770
                     /locus_tag="RD744_000636"
     CDS             8609..8770
                     /locus_tag="RD744_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556665.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9029..9463)
                     /locus_tag="RD744_000637"
     CDS             complement(9029..9463)
                     /locus_tag="RD744_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556666.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9573..11432)
                     /locus_tag="RD744_000638"
     CDS             complement(9573..11432)
                     /locus_tag="RD744_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDU0556667.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11550..12710)
                     /locus_tag="RD744_000639"
     CDS             complement(11550..12710)
                     /locus_tag="RD744_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDU0556668.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13017..14441)
                     /gene="araA"
                     /locus_tag="RD744_000640"
     CDS             complement(13017..14441)
                     /gene="araA"
                     /locus_tag="RD744_000640"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDU0556669.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14457..15155)
                     /locus_tag="RD744_000641"
     CDS             complement(14457..15155)
                     /locus_tag="RD744_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDU0556670.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15112..16773)
                     /locus_tag="RD744_000642"
     CDS             complement(15112..16773)
                     /locus_tag="RD744_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDU0556671.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16991..18391
                     /locus_tag="RD744_000643"
     CDS             16991..18391
                     /locus_tag="RD744_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDU0556672.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18643..19722)
                     /locus_tag="RD744_000644"
     CDS             complement(18643..19722)
                     /locus_tag="RD744_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0556673.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19808..20767)
                     /locus_tag="RD744_000645"
     CDS             complement(19808..20767)
                     /locus_tag="RD744_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDU0556674.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20779..22290)
                     /locus_tag="RD744_000646"
     CDS             complement(20779..22290)
                     /locus_tag="RD744_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDU0556675.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22312..22875)
                     /locus_tag="RD744_000647"
     CDS             complement(22312..22875)
                     /locus_tag="RD744_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDU0556676.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22881..23744)
                     /locus_tag="RD744_000648"
     CDS             complement(22881..23744)
                     /locus_tag="RD744_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0556677.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23758..24654)
                     /locus_tag="RD744_000649"
     CDS             complement(23758..24654)
                     /locus_tag="RD744_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0556678.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24829..26118)
                     /locus_tag="RD744_000650"
     CDS             complement(24829..26118)
                     /locus_tag="RD744_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0556679.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26090..27607)
                     /locus_tag="RD744_000651"
     CDS             complement(26090..27607)
                     /locus_tag="RD744_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDU0556680.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27805..28695
                     /locus_tag="RD744_000652"
     CDS             27805..28695
                     /locus_tag="RD744_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDU0556681.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28768..29592)
                     /gene="nadE"
                     /locus_tag="RD744_000653"
     CDS             complement(28768..29592)
                     /gene="nadE"
                     /locus_tag="RD744_000653"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDU0556682.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29604..31076)
                     /locus_tag="RD744_000654"
     CDS             complement(29604..31076)
                     /locus_tag="RD744_000654"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDU0556683.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9261 a         6643 c         5478 g        10275 t
ORIGIN      
        1 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
       61 ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca
      121 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga
      181 aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta
      241 ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct
      301 ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata
      361 atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt
      421 tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat
      481 gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt
      541 agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct
      601 tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt
      661 tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc
      721 caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg
      781 gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg
      841 ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt
      901 ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca
      961 ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg
     1021 gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg
     1081 atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc
     1141 aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt
     1201 acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc
     1261 gaaataaatt gagctttcca accagcggca tacagattct gattttcttt ttcaaatact
     1321 tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct
     1381 tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat
     1441 ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac
     1501 gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta
     1561 gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt
     1621 gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc
     1681 attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata
     1741 ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca
     1801 atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc
     1861 atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc
     1921 tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc
     1981 gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta
     2041 ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct
     2101 ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg
     2161 aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc
     2221 caaccagttt tccttcatct agcacaagga tcatatctgc atccattact gtacttactc
     2281 gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat
     2341 tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg
     2401 ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc
     2461 ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt
     2521 gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta ccatactgca
     2581 tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt
     2641 ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag
     2701 attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc
     2761 cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa
     2821 gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc
     2881 cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca
     2941 cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa
     3001 gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga
     3061 cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac
     3121 cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg
     3181 ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat
     3241 cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt
     3301 cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca
     3361 aaataatcat tactgggatg acaactaaga agatttttgt caactgatga tctttttgat
     3421 aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca
     3481 tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg
     3541 ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac
     3601 gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag
     3661 ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga
     3721 catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat
     3781 ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc
     3841 gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc
     3901 aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg
     3961 aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact aattggatca
     4021 gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc aattcaagca
     4081 actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt tggcgactcg
     4141 tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag ttacgataaa
     4201 aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag gattcgaatc
     4261 ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta gagctgtcac
     4321 gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa tactgataga
     4381 aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct tcttttaagg
     4441 gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt tctttaggca
     4501 aatggaaaaa tgtctgtgtc ggcatatgga tcctcctttc tttcaagatg acacattgtc
     4561 atatgacgct gtgtcattat aacacctcct acctgttttg caagacattt gcatagtttt
     4621 tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat ttgaattaat
     4681 gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat ggcccaatca
     4741 attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt tttagataca
     4801 agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat ccccgctgct
     4861 atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat tctaagctat
     4921 acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt tcgtgctgtt
     4981 tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt gcttgttaga
     5041 ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct gtcttttttt
     5101 agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt tctcatagtt
     5161 ataacaatca tagcaataaa aggagaaggt aagcatatgg catctgtttt agtagtcaaa
     5221 ggtcatcccc taactgcaga agaatcgcgc acagttaagg cattgacgtc ttttttaaca
     5281 agttataaag aaaatcatcc agatgatgaa gtgactgttt tggaacttta tcgcgatgat
     5341 attccggaaa tcgatgaaga attattatca ggttgggaag cattacgtgc tggtgcagaa
     5401 ttcacttcct tatcagaaag tcaacaacaa aaaattgctc gtttcaatga attgactgat
     5461 cagttccttg ctgctgataa aatcgttatc gcaaacgcct tatggaatct aaatatccca
     5521 acaaaattaa aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt ccgctataca
     5581 gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac tgcacatcca atctaatggc
     5641 ggtgtataca acggacaaga ctttgcctct caatatgtaa aaggtatttt aaactttgta
     5701 ggcatcgatc aagtagatca gctattcatc gaaggaatcg attatgaccc tgaccgtgca
     5761 gatgagttga tgcaaaatgc attagataaa gctgctgctt taggtaaatc attttaaaaa
     5821 ttgagataaa tgcatacaaa agagacagag gaaaaaatct tcaaagcagc atttgtacct
     5881 tggatatttt cccatctgtc tctttttgtt attctttctg ctttttcaac ggattttctt
     5941 cataaagaga agagattgtt gtgtaccatt caaaaacatg agtaatcact actttagctg
     6001 ctgcataaac aggaatcccc aagatcacac cgatgattcc gaacaatttc ccagaagtca
     6061 gcagaactaa taaaatcgtt actggatgga ttgctaactg attccctagt actaatggag
     6121 agatcaagcg tccttctatt gtctgctcta ctgcaaaaac gatcagtact ttgatgatca
     6181 tgaatggacc tgctacaatg gctaaaaaga tagccggaat catcgctaaa aatgatccta
     6241 agtaagggat caggttcaaa aaacccgcaa taatccccaa cgtgacagca tactctaaac
     6301 caatgaccgc aaaaccaatc ataaacatga ttgccacagc aaaagccaca gttagttgtc
     6361 ctcttatata agaagaaact tgactattca tttcactcag cacattcaac gtcggttttt
     6421 gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg tctttcaata
     6481 gataaaataa gataaacggc atcgtcagca gagccactaa aatagtcgct accgcgccga
     6541 caaaacttcc aagactctga actgtactct tagatatatc ttggatcata tcgcctgccg
     6601 aattcatgaa cttatcacct gctgtttcca actgttcgcg aaattggttg aacagcgggt
     6661 cccgcaaaat ctcggttaat ttattatcaa tcgtatctac atattgaggg aagttactga
     6721 tgaagctcac tgtttgttcc tgaatcttag ggatgatcac tacgctcccc catgcgatta
     6781 gtgcaacaac caaaacaaac agcccaatga tactgtaaag ccttggtact tttcgctttt
     6841 caaagtaatc aactaccgga ttcatcaagt aatacaagat tcctgctaaa atgataggca
     6901 gacctacgat tgctaaaaat tgccagattg gtgcaaacaa atatgaaact tttgtaaata
     6961 aaaagacgat cagcaatatt aacaatacaa caagtaaggc tgtgactact tgattattta
     7021 aaaaccagcg ccaaaaccac gataccggtc tgttcttctt attttttcct tccataattc
     7081 ctcctgttag ttataaaaaa tttggcttcc caaaccaata gcaggcagtc tatgcggatg
     7141 aagatagatt ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa tcaatgatac
     7201 gtgagctact gtctctaaat tcccattggc catcggaccg acataatcga tcgtatatac
     7261 ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt cttttgatac
     7321 ttcttgaatc accgaatatt ttcgtaaggc agctgttgct gttttatcga acaaaatcag
     7381 catgggttct tccttgttta ttgcttctgt cccaatttct gtaatctttg cctgaatcat
     7441 tgcactctct cctttaattg ctttcaaaaa cagtataaca cattctcatt ctttagccgt
     7501 gtggaataat tatgtgatag aataaaaacg aaaactattt cattacaatt aggaggaatc
     7561 agctcatatg atcgaaaaga taattttagg aacctatacg cgtcgcgaaa gtaaaggaat
     7621 ctatacgatc gaccttgata cagaaaaaga agaattagcc aacctttcat tagctgttga
     7681 agaaaatagc cctacctatg tagcaaaaag taaagctggc aatctttaca cagttactag
     7741 tgtggatgga ttaggtggtg ccggtgcgta cgataaggat tttcattttt tgaatgcggt
     7801 aacagagtct ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc aattactata
     7861 cggggcgaac tatcataaag gtgaagtaaa tgtctaccat atattaggag atggcgaact
     7921 gaaagcagca gattcaatat tccatcaaga agcgacaggt cctcataaaa accaagatca
     7981 tgcgcatgtc cactatacgg atctgactcc tgaccaacga ttagtcgtat gtgatcttgg
     8041 tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc ttgtaactac
     8101 cttcactgca gagccaggaa caggccctcg ccatcttgtc tttcatccaa atggacaatt
     8161 cgcttattta tttggtgaat tagacagcac agtccgcgtg ctttcttata ataaagaaac
     8221 aggtgctttt gaagaattac agaaaatatc gaccttgcca gaagaattcg acggtgaaaa
     8281 tggaggggca gctatccgca tttcaaatga tggcaagttc ctttatgctt ctaaccgagg
     8341 acataactcg attgctgtat tcgcagtttt tgaagatggt tcccacattc aaaacaagca
     8401 aatcatctca acagaagggg attttccccg cgatttcgca ctagatccaa gcaataattt
     8461 tgtcgtgtgt gccaatcaga acaccgataa tctgacatta tatcgccgtc atcctgaaac
     8521 aggattactg gaattactga aaaaagatgt ctatgtacca gaatgtgtct gtgtttattt
     8581 tgatcgttag taagaaaagg agaatgaaat gaatcagcaa gacgtattag acagactacg
     8641 ggaagaacta aatattcctt tctttgatgg gaaaatcgaa gataaagagt atagcgaaga
     8701 ggaatatcag aaactcaaag ccgatttaca gaactacttt gaacaatatg tacagaatgt
     8761 agaaaactaa tcagctttcc atactatctg aaaagaacat caggacaaga cttttccgtc
     8821 tatcctgatg ttcttttcta caaataaaaa accgccgaga tggttagact cgacggaaaa
     8881 ggagtattta ctcgattttg agtaagtaca aggttgttgt tggttatagg tatgtcatta
     8941 tcataaagga ttttgatcac tctatcataa aggtaatatt acatttatat tagataactc
     9001 tagtttttat tgttatcaaa gatttctttt attttttcaa caacaaatta gtcatttttt
     9061 ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat gtattgaata
     9121 gatcttcatc tacccctgat tcaagatcga tactttggat catttcttca cagcctgcta
     9181 catatgaaag ataagctttt tcaaaatttt tatgaatgcc cattgctttt gctggtggac
     9241 gcaaagtact gactttctta agcattgccc gatatttttt tgttccttct tcaaataaca
     9301 ggatgatctc ttcaagagtc gatttgtcca gttcacttgt ttttccttca tcaattgcgt
     9361 ttctgatcac ttcatatttc ggatgcattc cttctccgat tgcttctgtt tccttgacga
     9421 tttgatcgat ggactgcaca taatagccaa tattcggtaa catgttcgtt ttcctacttt
     9481 ctccattgtt gttattattt attctacttg tttttatcct caaatgacaa gtacttgaaa
     9541 aaatttaatg gtttctatac agctcgccat tcttattcgt gctcaaagaa accatctgct
     9601 aaactatatt gtttccctgc gacaatgtca tattggatca attgatattc gtcgtaaatc
     9661 tcttgttgcg agcgattgaa ttgcgcttct ttataccact gtccgttttg atagtacagt
     9721 tctcgttcaa acgccggaag ttcctgttct aatgataaaa gcagctgata aaagccattt
     9781 gttgtctgat tcgtctgttc cattagattc gctgcaaaat aaaacggact ggtgattgca
     9841 tcgtgagtcg tttgcttttc taattctcct ttactgtcga acataagaaa ttgtgtttca
     9901 tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg aatagatccc tggaagatga
     9961 tccccccaaa aaacaactaa agtccttcta gaaagattct tcaattcttc cgtgaactgc
    10021 ttcagtgccg tgctgctata ggaaatatct tgcagataat tctctaagtc aagtgtccca
    10081 cttccatctg aagtttttgc aggatagtct agattatcat actttccatc gtagggcata
    10141 tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt gagaagtgtc
    10201 attacttctt tgtaagctga ctcatcagaa atatatggat tattctctat cgtatccgta
    10261 taggtcatcg tacgctcgct gatgaactgg tcaaatccta atgtctgata aacatcctct
    10321 cttttataca tcgaagtatt gtatggatgg atcgctgtcg tctggtatga ttgtgcgttt
    10381 aacgctgaaa cgatagaagg aagctgatcc atttttggta caagcatcgt gtaaggggtg
    10441 gtgagttgcg cattaaataa cgccatagaa aaaccagtca atgcttcaaa ttcgatattt
    10501 gctgttccac cgccatagtt ttgagaaagc atattgccac tatatgtttg atcagctact
    10561 tcataataat ctgccaatgg ctcaccagat acttctacgc cgtttaatcg actaggatct
    10621 gagaaacttt cactcatcac gaacacgatg ttcggcgact cttcctctac agttttattt
    10681 ttttcatcgg ctagcttctg gtatttttca gtgatttctt tgatcttttc tttcgaatat
    10741 ccttcaggtt catccattgg ttctaccttc agattgtaca aaaacccacc gataaaacca
    10801 gtgttatagt agttcatttt ctggctgtaa ggtatccata gtgccgtttt attatatgct
    10861 tttcgcaaca aattatcggg attgttgaaa ttactgatat accctagcag tccaattgcc
    10921 acaagcagtg tcgttaatcg gatcaattgg aatcgccttc cttttttcag gcttcgaaac
    10981 atgtaccagc aaaacaatcc tatcacggtt cctgcggcag ctaagatcac tgtaaacagc
    11041 atagtgccgg tcatatcttt cagcaatccg atttcagtga tcatcttcaa atcgtctgga
    11101 taaatcggtt cttgccgata actcattttc atataatttg caaaacctaa tacaccgatg
    11161 gctacgacat acgtaaggga acctaaaggg acagatcctg ctagtgcaat cagaaaaata
    11221 agaataatca gcaagacgag acaggctaag aagaattttt ccgtatgcca agaaaaagca
    11281 aatttcaacg caaggtctac ggatagatca ttttgacacc actgcaaaaa taaattacta
    11341 aaaacaacaa gaaaaaagat actgatgatt gctgctgttc cttttaaaat ctttttctgt
    11401 ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc tccttcattt ctatcaataa
    11461 aagaatgttg cttctcttat tttagttgta cgaaattcga aagtaaagaa cggaaagaag
    11521 aattaatgaa atccaaatat tctctttctt tatggccttt ctggtttctt taataagcgg
    11581 acctttcgta ctccagaaat tttttctagc tgttgtttca cttgattgag ctgttcaaaa
    11641 tcagtactgt ctatgtcaat caatatgtag acatgctcat tcttatggtt acgcgatata
    11701 tctgcgatcg ctagctcgtt ctcattcagc agagaaaata tctctgctaa tatattggtt
    11761 ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg tgcggatgga
    11821 taattgaccg cttctctaac cgtaccgaac aaaagataat cttttaaggc ttcaactgcc
    11881 aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc ctagatgagg caacataagt
    11941 atacggtcat tttctaagaa ctcttcttct ggaaaatcag tcaagtattt agccagatgt
    12001 tgtttttcca atgcttgcat aacagcatat ttatcaacaa tttcacttct tcctacattg
    12061 ataagtacag catctttttt cattttttct atattcgcct gatcgatcaa tccttttgta
    12121 tcttctgtca acggcagcat gactacgatg taatccgagg tagaaagaac tgtttctaaa
    12181 tctgcttgcc gaacataatc caactgggca tcgcggatag aataacccaa gacatccatc
    12241 cctaagctat agcaacttag cgctactttt gtgcctatcg ctccaagacc aagaagaccg
    12301 atggttttgc cttgcaattc tcgtcccaca taagcacttc ttttgttttc tgcctgttct
    12361 aaaatattag gacctgttag tgtttgtacc atacgacttg cttcgattat cggacgggaa
    12421 gataagagca aacagcacaa aacaagctct tttactgcat tagcatttac acctggtgta
    12481 ttcatgacga tcgtcccgtt ttcagaagct ttttcaacgt tgatcgtgtt caccccaaca
    12541 ccagctcttg aaattgctag caaatctggt gtaagccatt catctttcac tttcgtacta
    12601 cgaataacta ttccttcggc tggcaaatca tctattattt tgaaatattc cccttgtaac
    12661 ttttctatct cttgttctgg aatagcatca atcaaatgaa cgtgatacat aaaaccccct
    12721 ccttactaac aagtatctcg aaatttctgc aattagacaa ctgtttacca tttgacttcc
    12781 tagaagcaaa aagagcagtt agagcagagt aggctgagtg ataaaaataa ggctatgaca
    12841 agttactgcc ataagcctta ttttcgagag caaaatattg agggtgagat atcttgataa
    12901 atggtgttcc gttagctgac attgataata agactaaatt tcaaaaaagt tgaaaagcaa
    12961 atttcgtcaa tctcttctta tttcttaaaa ctaagtggct ttttcacaac ctctttttat
    13021 ctaataacca ctgtttccaa ctcaactaat tttgcccacg tttcaatttg atctgttgtt
    13081 agatttagcg agacaacggt atgatgaccg ccacctgcct gaatccatga atggacacct
    13141 tcatggaaat tcggtttagt tttccataac acacgtgcaa caggtaaatt aggtgcttca
    13201 gtcgttggtt caaaggcttc tacttcattg atcagcagtt tataatgcgt accgaaatct
    13261 gccatcgata cgactacccc ttctcctgct ttcccatcaa atacaagacg cgccggatct
    13321 tctctatctc ccattgataa aggagaaaca acgattcttg gtttcgtcgc agcaagcgtc
    13381 ggatctactt ccatcatatg ggattccaag attgcttctt ggccactagc tagctcgtaa
    13441 gtataatctt ccatgaatcc tgtatctttc cctcgagcca tgattttcag caaccgatca
    13501 atcgctgcag ttttccagtc gccttcccca gcaaaaccgt atccttctgc catcaaacgt
    13561 tgtacagcta gtccaggtag ttgtttcatt ccatacaggt cttcaaaatt agtcgtaaaa
    13621 gcgttatagc ctccttcttc taagaaacgg cgaataccga tttcctgctg agcttgtact
    13681 tttacatgtt cctcccatgt cttttcttca taatcaccat agtcgaaggt atacaattcc
    13741 ttgtactctt caaataattg atcgacttct tcgtctgata cgcgatcgat tacttgtaca
    13801 agatctccga taccaaaata atcaacagtc caaccaaatt gaatctgtgc ttccactttg
    13861 tctccttctg tgacacccac gtttctcatg ttgtcaccaa aacgagccac ttttattcca
    13921 aaactttcat tataggctac tgcaacatcc atccaatcag caatctcttt ttggatctca
    13981 ggtcgtttcc agtagccaac gacgatttta ttttgtttat ttaatcgagc attgatgaac
    14041 ccatattcgc ggtcaccatg agcggattga tttaaattca tgaaatccat atcgatcgtt
    14101 ttccaaggaa tgctttcgtt atattgcgta gctaaatgaa gcaatggttt ttgtagaagc
    14161 tttgtcccgc ggatccacat ttttgctggt gagaaagtat gcatccaagt gatcacacct
    14221 gctacttctt ctcgataatt gacctctttc atgatttttg tgatcgtatc agcagtaact
    14281 gctaattctt gaaggcggat tggataaggc aattggccat tttcattcag ttcatcgacc
    14341 atttcttgtg catgtttctt tacttctcgc agtgcttctt ccccatataa atgctgtgat
    14401 cctacaacaa accaaaattc tttttctcca atattcaaca taattagcca ctccttttat
    14461 ttgttttgtc cgtaatacgc attttttccg tgttttcgta aataatgttt atctaaaatc
    14521 cgttgaggca gctcttctga aaatgaattg agctgtctgg taaataaatt catcttgcat
    14581 acttcatcta gtacgactgc gttcattacc gcactttgcg catctttccc ccaagtaaat
    14641 ggaccgtgtc cgtgtaaaag tacccctgga attgctaagg gatcaatttt gcgttctttg
    14701 aatgtttcta tgataacttt tcctgtttca tattcgtacc cgctgtcgat ttcctgttgt
    14761 gtcaaaaatc gagcacaagg taccgatcca taaaaagtat ctgcatgcgt cgtccccatc
    14821 gcaggaacat ccaatcctgc ttgtgcccag atcgtcgccc atgtcgagtg agtatggacg
    14881 attcctccga tttgaggaaa tgaacgatat aaaacagcat gagtaggcgt atccgaagag
    14941 ggattgagtt taccttcaat gatatgattg tccaagtcca caacgaccat gtcgtctgct
    15001 gtcatctctt cataactgac tccgctaggt ttgataacga aatatcgtgt ttcaggatca
    15061 aaagcgctca catttcccca tgtatatttg acgagtccgt gtttaggcaa gtcaagattt
    15121 gcttggtata cttcttcttt cagttgttcc agcattttct atcactcctt tttcttgcaa
    15181 ttgatgaatc gctgtttttt caatgtcgat cccctcacgg taacgtctca taaatatctg
    15241 atagcctgct atttcttctg ctgaaggtgt gatggtgatt ccttggtctt gtccaaacac
    15301 gtcttctgcc aagaagtttt ctaaagactt cccatgtcga tccatcatat aggctgctaa
    15361 taacgcgatt ccccacgctc caccttctcc ggctgtgtcc atcactgtaa ctggtgcctc
    15421 catagcagat gctagaatcc gctgtccaac ttctggtgtt ttaaaaatcc ccccatgccc
    15481 cactaaacga tcaatttgga cattctcaga tttcaatata tccattccga ttctcattgc
    15541 accaaaagca ctggctaaat ggatccgcat gaaattcgct aaatcaaaac ggctacctgg
    15601 tgtacgtaca aataatggcc ttccttcatt tactcctgta atgttttcac cggaaaaata
    15661 accataagaa agtaatcccc cgcaatccgt atcgccttct agcgctttgt tgaataatac
    15721 cgtgaataat ttatctctgc ttatctccat acccaaagaa ttggtaaatt cttcaaaaat
    15781 actgacccaa gcattgatat ccgaagagca gttattcgta tgaaccatgc ctactagttc
    15841 tcctgaaggt gtagtgacca tgtcgatttc cggatggaca ttttctagtt ctttttctaa
    15901 tacgatcata gcgaaggcgg aagtaccagc cgaaatgttc cctgtccgtt tggctacact
    15961 gttcgtagcg accataccgg ttcctgcatc cccttctggc ggacaaaccg ggattcctgg
    16021 tctcaaattt cccgtagggt cgatcagata agcccctgat tcgcttaact tgccagcttc
    16081 ttctcctgct actagaactt taggcaaaat gtgatccaag tcaatcgcta cgtgcgcttt
    16141 ttcgattaac tgaccaaaaa tatttttcat ttcttgatgg taggttttcg ttgaaggatc
    16201 gataggaaac atgcctgatg catctcctac gcctagtact ttctcaccag taagctgcca
    16261 atggacataa cctgcaagcg ttgtcaaaaa attaatttct ttcacatgtt cttctttatt
    16321 caaaatagat tgatacaaat gagcgatact ccatctctgc ggaatattgt attgaaattt
    16381 ttccgttaat attcgttctg cttcttcagt gattgtattt cgccaagtac gaaatggtac
    16441 tagtaactgt tccttgctat caaatgctag ataaccgtgc atcattgcac tgaacccaat
    16501 cgacccgatt gtcgtaagat tctcgccata gatttcgaat acttctgctg ccatttttcg
    16561 gtaactgacg cgcagtcctt tccaaatatc ttctaatgaa taggtccaga taccattttc
    16621 tagtttgttt tcccactcat aactaccagc ggcaatagga gtaaaagatg aatcaatcaa
    16681 tacagctttg atcctagttg atccaaactc aatccctaaa gatgtacgac catctatgac
    16741 atctaacata ctttgcgcat ttacttcctt cataatgatt taactccctc cttctcgtca
    16801 aacctgatca aaccgtcttt ttagtagcgc ttacaatgat tattgtatac ttttgtacgt
    16861 acatgtcaag cggttattta caagaataca ataaaaaaca cttgttttgt acgtacataa
    16921 gttataaaat gaaattgctt acaaaatctg atcaaccgcc ttctcgaaat tctagaagga
    16981 gagaatactt atgaaaaaag aaaaaaaagt ttcctcatcg tttatttatt tttttggcgc
    17041 atttggcggc attctatttg gatatgatat tggtgttatg accggcgcct tacctttttt
    17101 acagcatgat tggggtttgg caggtaaagc ttctttgata ggatggatca cgtcttcggt
    17161 catgttaggt gcgatccttg gcggctcttt atctggtctt ctttctgata agcttggacg
    17221 acgaaaaatg attcttcttt ctgcacttat ttttatggct ggttctgtcc tttctgcatc
    17281 tgcccctcac aatggttctt attttctgat tgcagcaagg attcttctag gtttagctgt
    17341 tggcgctgca tctgccctag ttcctgctta tatgtctgaa atggctcctg ctagactaag
    17401 gggaagattg tcagggatca atcaagtgat gattgcttct ggcatgctcc tttcctatgt
    17461 tgcagattat ttgctaaaag tgttgcctga aacgatggct tggcgcgtga tgttagggct
    17521 cgctgctgtc cccgcgctga ttttattttt cggtgtttta gctctaccag agtcaccgcg
    17581 ttttcttatg caatctggcc gcttggaaga ggcaaaaaga gtattgaact atattcggac
    17641 accaaaagaa gctgaacagg aattcgaaca aatccagctg aatgtcaaac aagaaaagac
    17701 aaccggaact tcttggcata ctttgttttt agaaaaatat cgttccttag tcattgccgg
    17761 tattggtgta gcagtattcc agcaatttca aggagctaat gctatttttt actatattcc
    17821 gctgatcgta gaaaaagcga ctgggcatgc agcgagcgat gcattgatgt ggccgatcat
    17881 ccaaggaatc attttagttg ctggttcttt gctctttttg gttattgcag acaagttcaa
    17941 tcggcgaact ttattgaaaa taggcggtag cgtgatggga ctttcattca tcctaccagc
    18001 tgttttagga actgtactgg atgctcatac aaattcgttg ttgattctct tgtttttatg
    18061 tatttatgtc gcattttatt cttgcacatg ggctccactg acatgggtaa tcataggcga
    18121 aatattccca ctagctgtcc gcgggcgagc atctggccta gcctcttctt ttaattggat
    18181 tggttctttc ctagttggct tgctttttcc tgtgatgact gcttctatgt ctcaagaaat
    18241 cgttttcggc atttttggga tcatctgctt tttaggggtt ttatttattc aagagatcgt
    18301 accagaaact cgtgggaaat ctttagagga aatcgaacaa agtgcatcta aaaaaactta
    18361 tcctaaacgt atttctatcc atcattctta aagaggttgt gacagaagtg gtcagcttca
    18421 agaaataaga aggaaccgct tagtttccag tcacaaggaa caatttcaaa aaacagcttc
    18481 tcatgtttga tgaatattgt gacttgtgac cagttggtag gagaacaccg tttagtggag
    18541 aggtggtgaa agaaacgttc agccttgagc gatgacaaaa gctctcacaa acattgcgac
    18601 gagtaaccgt aggagcaata agaggcggta aaaacccgtc tcttattttt cgttttcttt
    18661 cactgtttcc cgttcaacta gtacaggttc atagagaata tttggctgtg cctgtccttt
    18721 ttcaatcgtt gtgacgatcc accgtgcggc atcttgtccc aacttttctt ttggatgcgt
    18781 caatgttgtc aattttttat tccctaaatg gcttaaagaa gaatcgtctg ttcctacgat
    18841 cgataggtca tctggaatat gatagccatt ttctgtcaac ttatctagca ttaaactagc
    18901 tatttgatcg ttatagcaaa tgatgccatc taaagcttgt tcttctttca accgttgtac
    18961 tgttttttca gcgattgttt ctcgtgtttc cgtggtgaaa gtaatgatat cttccgtcaa
    19021 aaaacgcaca ttatactgtt cacacgcgct gataaaacct ttcatcctgt attttccttg
    19081 aagatcgtcg atttttgtga tgaacaacag acgctgatgt ccttttttta tcaaatattc
    19141 tgctgccata aaaccgactt tcacatcatc taaacaaata taagaggcat ttaactcttc
    19201 ataagcagca ttgatcatca ccatcggaat gccttgttct ctcaaagcaa cataaagtgc
    19261 taagtttggg ttatactgat tacttttcgt aggctcaacg atcaaaccat ctaccccata
    19321 agcaatcatt ttcttcaaac actctttctc caattcgtga tcgttattcg tactggctaa
    19381 aagcaatgaa tacccagatt ctcgtaattc attttcgatt cctcggataa tcgacggaaa
    19441 aatataatca gagagatatg tggtgatgac accgatcgtt tttgttctgt tcaatggtgt
    19501 tttttctttc ttatagctgt catctacata tgtgcccgac cctttttctt tacgcagata
    19561 gccctcattt accaacacag cgattgcttg ccgaatcgta tgtctgctga cgtcatatat
    19621 ttcttgcagc ttgaattctg agggaatggt tgtatttact tcgtattcct cacgaaggat
    19681 cttttgtttt atctcttctg caatatcttg atattttgcc atttgccttg ccccttttta
    19741 tttgtacgta catatatcat aacgagtctg caaggtttat tcaagcaaaa atcaaggttt
    19801 cttgtgctca taaggcatac ggaacaggtt caccaaattc tggaaatcct tgctcattcc
    19861 acgtaaagca ttgcgcgttc gcgtgccgat taggattgtc taacggatcc cctttttggt
    19921 tcttctctgg tcgagcatga tagatcaaaa tatcaaagca cccgtcttct gaaaccgtaa
    19981 acgagttgtg accaggacca aattgcttat ttttttcaga agaaacgaat actggctccg
    20041 ataatttatg ccatgattct tttaataaaa gattcttgtg ctcatcagcc cacagcaggc
    20101 ccatggcata gttttcatct gttgcactac cagaatacgt aatgaatatt ttcccattgc
    20161 gagtaaggac tgctggacct tcatttactg aaaaaccccg tttttcccat tcatactcag
    20221 gtatagaaag gaggatctgc ttccccttta acgtccaagg attatccatt tcggatagat
    20281 aaagattaga attcccagta atttcaggat cttgttgggc ccacacataa tatagctttc
    20341 cttgagaaga gaaagttgtt ccgtctaaac tgaaagtttc ccttttcgtt tttatttgtc
    20401 ccttttctat ccaatctgta ttcattggat ctatatgctg attttcaagt acgaacatgc
    20461 ggtgatgatg agattcatca cgtatagttt tatgattgct tgcagcaaag taaatatacc
    20521 attttccatt gatataatgg acttcaggtg cccagatcaa ttcactcatc ggtccttctt
    20581 catgagcctg ccatacaatc aggcttgcag catgttctaa ttcattcagt gactttgccc
    20641 ttcttaattc aatcacttgg tagcctggaa ccgatcctgt aaaataataa taaccatctg
    20701 tatgtttata tacccaagga tctgcccgtt gccgaacgat aggattatta taaagtgtct
    20761 tcatcataac aaatttcctc acattttctt tattctaatt acattccaag agtatttttc
    20821 tatccctaca tttaattcgt catgtttttc tattgaaaaa tagtttattt caggttttat
    20881 tttttctggt tcttcacgtg tatttttgtc caacagatcg ctactggtta atgtgtagtg
    20941 ctctccttga tctagcaaat tgaaatcatg ttcaatcgtt atcttttgtt gttcatttgt
    21001 gcgattgatc aagaaaatag tcagctgatt gccgttatct acgatcacac tgtccacata
    21061 aggaacattt tttatctttt cacaatcata aacagggcaa atgatttgtt gtttcaatac
    21121 ttgaccttta gcatatttcg ataagtgttg catcacataa taagttggat tcctccatgc
    21181 ttgtccttta gcatctgtca gtattaaagg tattgtgttt actaacaatg attgacaact
    21241 aatctttacg cgatctgcgt gtcgtaaaat agccagccct aatgaaccaa ataataatgt
    21301 atcttccata gtaaagtaac accaatcgat gggactaccg acttcaaaat ccctatgtct
    21361 tttttccggt tgggcatgta cattccattc atcaaacgct agatacattg ttttctcact
    21421 tctcaaatat ccttttacat aatcacaagt agcaacgatt tcttcaattt gacgatcgaa
    21481 tcgataagct tttgcaaggt atgtcgctgt atcatcacgc tcatttttag gaggtttagt
    21541 caagtcttct tcatcatact tatcaatata gttatgcaaa gctaaataat ctacattctc
    21601 ataagcttct tctaatgcga tacgatccca ttcagggtac gatggtaaac gtggcgtaga
    21661 gcttccaact aagattgttt caattgaatc atccactaac ttcatgactt ttgaggcttc
    21721 atttgctaac ctaccatatt cataagcact cttagccccc acttgccatg gaccgtctag
    21781 ctcattccct aaacaccata atttaacgcc aaaaggcttt ttttgcccat tttttattct
    21841 taaatcactc cagaaggttc cttttggaaa attacaatat tctactaaat tttttgcttc
    21901 atcaattcct tttgttccta aattcaccgt gaatatcggt ttcgcttgta catgactgat
    21961 ccatttaaaa aattcgtgga gtccaaaaga atttggttct atacctcgcc atgctaaatc
    22021 aatttttttt ggtcgttgtt ctttcctacc tacagtatcc acccaatcat aaccagatgt
    22081 aaagtttcct ccaggatagc gaatgacacc taattctaat tcctttacat aagagaggac
    22141 atcttgtcta aacccgtcac catcagctgt ttcatggccc ggttcataaa tccctgtata
    22201 gacgacactt cccatatgct caacaaatga gccaaacagc cgagaactga tctctcctac
    22261 cgtaaaatcc tctctgattt ttactttcac ttcttcgtcc aacagctttc cctacctttc
    22321 atttcctata gtcaagataa tagatacaag tttccatacg ccaccagcaa agataaaaat
    22381 aaaaggaggg aaaagaaata tgagcagtac aaatcctaat aacaatacgc aacaaagaat
    22441 atttctcaaa aaatcaaaaa atactttcca tgcactttgt tcaaaaattt gtaaaaatgt
    22501 gatatctttg ttttcagaaa tcacatatat ccaggataaa agaaacatgg aataaataag
    22561 taaaactaca tatccactaa ttgaaagtac gattgtctgt tcttctaata attgattaac
    22621 aagaaaataa ataatgatcg ttaacagact aaggaacaag ttcttaccaa aatgttgctt
    22681 tattccttga aagaaaaaat aaatgatatt tcctgatccc tcacctttcc attgcgccat
    22741 tacataaaaa acagcacatg tagcaggcac gatagtaata acaggcaaac acacgattac
    22801 ccagagaata cctaataaga tgtaatcaga aaccacaatc aactgtccat aaaatttagt
    22861 ctcaactatt ttcatcttcc tcactctttc attccagaca tagctgttcc ttgaacaaaa
    22921 tatttctgtg caaaaatata aaccactagt aatggcagca ttgaaataac cgttccagcc
    22981 attacaggtc cataaaaaga aatgttctgc cctgtaaata aagctaaccc tgctggtaaa
    23041 gttctcattt ttgaactact tgtcaatatc aatggataaa gcaagtcatt ccaactattc
    23101 atcagcgtga aaatccctaa tgataaaaga gtaggtttgc tcaatggcaa catgattttc
    23161 aaaaaaatat taaactcact taatccatca atgcgtgctg cctcttccaa agaatctggt
    23221 aaagtaataa aaaaggaacg catcatgaag ataccaaatg cagttgtcat acgaggaatg
    23281 atcaaacctg cataagtatc aagcatgcca agcaaattga cttcaataaa tagaggaatc
    23341 atgaagactt ggaatgggat catcattgtc agcaaaacga aatagaacaa aactgatttt
    23401 cctttgaaac gcatacgagc aaatgcatac cccgcaagag aatcaaagaa tacagaagtg
    23461 atcactgtcc ctccagcaaa aatcactgta tttttgatgt aatctaataa agggatcgtt
    23521 gtccaaacct taatatagtt atctagggta tagctatctg caaatattct aggcggaaat
    23581 gagataatgt ctttttcgtg tttaaaagaa gaaataatca gccacaaaaa aggaaacgca
    23641 ataaccaaca atataactag catactaatc gttcgaatga tttttttacc actaactgct
    23701 tttcgactat tcacagtaac tgtaatcgag ctttctggtg ccatacaggt ttccctccta
    23761 tttcatttgt ttttctttcc gtgacatgta cacattagtg gagatagcta tagttgcaac
    23821 aatgataaac aaaaccgaag acagtgctga tgcatagccc aattcataag gtgctgtaaa
    23881 tcctcgactg taaatatatt gaacagctgt ttctgtctta aattgtgggc caccagcggt
    23941 tgcaacatat acttggtcaa acacttgcat cgaaccaatc agattcgtta aaatacaaaa
    24001 acctaaggag ggaacgatct gaggtagcgt aatgttaaaa aactgctgtc gatttgttgc
    24061 accatccatt tgagcagatt catacaaaga gggagagatt ccctgaatag cagtcaagag
    24121 aatcgtcata gtaacaccaa aattcttcca aacagtcatg atggctaccg tcggcatagc
    24181 aagggttgga tcgcgaaaaa attgagggat cggcaagccg acttgtgaaa acaagtaagg
    24241 aatcgcccct acattcggat caagcaacat agaaaaaata atcgcaattg acgtcaatga
    24301 acaaataact ggaatgtaaa atgtcgaacg acaaaaacga ttgaatctgg tatttttaga
    24361 taatgctact gctgctaata aaccaacgat aatttgtgtt ggcgtttcga gtacggtgaa
    24421 atataaagaa tgaatcaacg aattaactgc tcgttcatca ctaaatatac gtgtgaaatt
    24481 ttcaaaactt acaaaatttc ttgatgtaaa gaaaatattc aaatctgtaa aactaatagc
    24541 aaatgttcct aaaagcggta tgacagtaaa taataataaa actaaaaaag atggagttaa
    24601 aaacaaatac gcagcacgat caggacgatt ccaatatttg gtgaatccgt tcatatagtc
    24661 cacctcatct ttcattttga taaaatatac aatcttggtt taaacgaatc tgaataagat
    24721 atccgaacaa tcgctgtgat ttttgcaata acacctatct gcaaatacta ggtcgatcat
    24781 tcagatatct gtatcttgcc aacagtctat cctgtataaa gttctgtact atttttgcag
    24841 taattgattg atggcatcat ctgcatcgtt catgagtgtc tcaggatctt ctcctgctaa
    24901 taatttttca atcagtggat tgatgatatc gttattgatc gcagagattt gggtaaaacc
    24961 tggtaaatat ggctctgcat agtccatttg tttcgataat tcagatacaa taggatcatt
    25021 ttttacttct tcatcttttg ctactgaatc caagtaggca ggaaaaccat tttcgatact
    25081 ccatttttta ccaatttcag ttgtattcca atatttcaaa aactcataga cagcttcttt
    25141 tttattatta tctgtacttg ccggaatggc atatcctacg ccatctaaaa tagctgtttt
    25201 tttgtctgaa tcactccctt gtggtaaggt agtcacatta taatcaatct cgtttgcttt
    25261 caatccgtta tttaaccatg gcccattgat ttcaatggct agttgaccag cattcattaa
    25321 attgtccatt tcggcgccag aaatcgattc tggaccagtc ttgtctacat ggatcatttt
    25381 ttggatcgct tttaacgttt taaggttctc aggagacgca aattcagact tagtataatc
    25441 atcgttcact aatttaccgc catttgctaa catccagtta tataacggtg cagttccatc
    25501 cttattgaag acaaaacctg atacattttt actactatca gttaattttg gtgccatttc
    25561 tgtcaattct tcccaagtag taggtggttg ttcagtatct aatccagctt gtttaaacaa
    25621 atcattattc caatacatat acatgccttg tacctgcatg gggacaaaat actgtttacc
    25681 ttctacttca cctaattcaa tagccgtttc tttaaaatca gatttatcta ctccatcata
    25741 ttcccagaaa tcgtctaatg gttgtaccgc tccgttttct acatactgtg caaaatctgg
    25801 ataatttaat gcaataaagt caggtgcttt tttagaagaa atcgctgtag gaagtttttc
    25861 attcaaattg gcccaagtca tgatatccat ttcaattttg atgtttttat catttgtttt
    25921 attaaattca ttaacgattt cttgcaatat ttcaccatca gatgcagtaa acccattcca
    25981 aaaagtcaaa gtcacttctt tatcagaatc agattctgct gaacttccgc caccgcaagc
    26041 tcctaaaata cctgttgcag ctaccaacaa agagccaatt aaccattttt tacctttcat
    26101 tttttgttcc tccttcatct gataaacgac atctgaccac attccaagat agcggtttca
    26161 ataaacaatt gattttccca tcttcagctt ttacatttgt tgaatgactt ggttttactt
    26221 gttcactttt aggaagatta gttgcttttg tatcaaatcc actcatttca gaaaaatcaa
    26281 tcactccctc taatgataat tcagacaagt gaacagttag attcatttct tcttctccac
    26341 ggttaactgc aaatatgaca agttctttgt catttattac ggctatactt tctaaatacg
    26401 gcacttcttc aaaatcttcc gtcgcatagt ttgctgaacg aatttgtggg cttaacacct
    26461 ctccttgtcc atacacagca gcttgcataa atggataata gatcgtctgt ctccaagctt
    26521 ctccgtttgt ttctgtcctg attggtgcaa taacgtttac caattgagca agacaagcaa
    26581 tctttactct atctgcatgt ttcaataaag tgattaatag acagcctaat agtaatgcat
    26641 cttcaaaatt atagttatct tctagtaaag gaggagcaat ctgccaaggc gctgcttgct
    26701 catcttgttc cgctgaatga taccaaatat tccattcatc aaaggacagg ttgatctgct
    26761 tcttactatg tttcttcgcc ttgactgtat cgcacatcgc tgtcacttca gaaatgaacc
    26821 gatccatgtc taaggaacgt gcaagataat gagaaagatc atttttttgg ttgccataat
    26881 attgatgcag agacaaataa tctatttgat cgtacgcttg atcaagaact gttaactccc
    26941 aatcaccaaa tgtaggcatt tgccgattcg aacttccgca taaaaccacc tcaattgaat
    27001 catctacgag tttcatcgcc ttagccgttt cctctgctaa ccgtccatat tcttccgctg
    27061 ttttatgtcc aatctgccaa ggaccgtcca tttcattccc taaacaccat gtcttaattc
    27121 caaaagggct ctcttgacca ttcgaacgcc ttaaatcact ccaataagtt cctttaggaa
    27181 aattacaata ttcgactaaa ttacgagctg catctactcc acgagtaccc agattgacag
    27241 ccatattgat ttcggccccc acatgctgac accagttata aaattcatga atcccaactt
    27301 cattcgtttc aattgtccgc caagctaaat ccaaccttct aggtctattt gctatgggcc
    27361 caatactgtc ttcccaacga aatcctgaca caaaatttcc accaggatat ctaatcatgg
    27421 gaatgttcaa ttcttttacc aagtgaatga cgtcttggcg aaatccactt ttattagctg
    27481 atggatgatc tggctgataa atcccttcat atactgctcg tcccatatgt tcaataaatg
    27541 aactgtacaa ccgcggatca actttgccta cacgcttgac actactaatc aacattgtgg
    27601 cttccataac tgacctcctt taatttatac atgaataata cacctcgttg aaagcgttgt
    27661 caataatata aatgttaaac ttacttattt tttatcgatt ttaattaacc aaaatattaa
    27721 taatgattaa aacaaataat ttaatataac ttttattaac aaatatgtta atatataaga
    27781 aaaacctttt aagaggtgat atatatgcaa ctacaaataa acgaagaatc cttaccggta
    27841 tatgaagcat tagctagtaa aacaaggatc aagatcatcc agctactttc caaaaaaaag
    27901 atgaatgtca aagatttggc aaaagaactt ggagtgagca gtgctatcac gacgatgcac
    27961 gtgaaaaagc tagaggaagc aaacatcatc aaaacagaaa aagtaggaca acaaaaaatt
    28021 tcgagccttc gtgtcgataa aatcgatatt agttttccag aaaaaatatt caatgctttt
    28081 gatacgaaag aaacatcgat acctattggt cactatacaa attatgcaat tgaaccaaca
    28141 tgcggtttag ctacgatcca tgattttatc gggaaagtgg atgaaccacg ctattttatg
    28201 gatcctcgaa ggatggatgc ccgcattctc tggtttacca gcggatttgt cgagtatcag
    28261 gcacctaatt ttttaaatac agaggataca ttggagatgt tggaagtttc tgtggaaatt
    28321 tcttctgaat ttcctttttc aaatgacaat tggccttcag atatcacttt cagcctaaat
    28381 ggtgtggagc taggaacatg gacgagccct ggagattttg ctgatatccg cggtaaatat
    28441 acgcctgatt ggtatccaga taatcttaat caatacggct tgctgaagac gatccgaatt
    28501 accaaacact tgacgaatat gaatggagaa cccttgtcaa acatcacgat caacgacatc
    28561 cctaaagagc aagacacttg gcatttgcga atcgaagtga aagacgatgc caaacatgtg
    28621 gggggctgta ctttatttgg taaaggattc ggtaattacg atcaggatat caaattaaaa
    28681 gtttactaca gctgatattt ttcttttcta ttttataaaa caaaaacagc caatccctat
    28741 gaagggacgg ctgtttttgc tttattgtta tttccagaaa tcatccaaaa cggaaattgg
    28801 caaatgtcgt ttatgttgtg tcttgttcca ccatgcttcg attttttctt gtgcttgagc
    28861 gggaacttct tttccttcta gataatcatc aatttcatta tatgtgacac ctagtgcgac
    28921 ttcatctgca atcattggtt tcccatcttc aagatctgca gtcggaattt ttgtgtataa
    28981 tttttccggt gcattcaatg cttgaagcaa ttgtttccct tgtcgtttgt ccaaacggaa
    29041 taatggcaaa atgtcggctc caccatcacc aaatttcgtg aagaatccag taatgttttc
    29101 tgcagcatga tctgtcccta atacggctcc tgctctttct ccagctacgg catattgcgt
    29161 aatcatacgt tgacgtgctt tgatatttcc tttgttaaaa tcgctgattc ccacacctgc
    29221 ttcgcttaat acttgtactt gtgcatcaac agctggtttg atatttactc gcagactaac
    29281 atcgggctgg ataaagtcaa gagcagcttt tgcatcttct tcatctgctt gttcaccata
    29341 aggaagacga acagcgataa attgatagtt ttcatctttt gtttcttcac gcatttcttc
    29401 catcgttagt tgcgccaaac ggcctgctaa agtagaatct tggccgccgc tgatacctag
    29461 tacaaaagtt ttaagaaacg gatatttttt caaatatgct ttcataaagt caatactttt
    29521 acgtatttct tcttttggat caattgttgg tttgacacct aattcattga taatttctgt
    29581 ctgcaggctc atttattctc ctcctatgct tctgtatgaa gttctgctac gctttttttc
    29641 attcgttcca tgatagccat tttatggttc cagcattcag tagacagatc gactgggtat
    29701 ttttgcggat tcaagtctcg tttatactct tcccatagtg aatctagatt ttcttttgta
    29761 tattctttga tttcgtttag tgcaggtaat tcgtaaactc tttttccttc gataaagata
    29821 tcttgtaaaa ctgggcgagc tgtaaagtct cgaacagttt tattgataaa cgtatgcact
    29881 ggatggaaca tgaagatagc ttcttcttct cgagggtctt catcccataa tgtcacgtag
    29941 tctccttcag atttcccatc aaaatttcga gtgatgcgcc acacttgttt tttccctgga
    30001 gtagttactt tttctgcatt gcttgataac ttgatcgtat ccatcatttt tccttcatca
    30061 tcttcgatcg atactagctt gaatactgca ccaagggctg gctgatcata ggctgtgatc
    30121 aattttgtcc caacacccca tacatcaatt ttagcttttt gcatctttaa attcaaaatc
    30181 gttgcttcat ccaagtcatt cgaagcatag atttttgctt ccgtaaatcc ggctgcatct
    30241 aattgttccc gaacacgctt agatatatag gccatatctc cactgtcgat gcgtacgcct
    30301 tggaagttga ttttgtcacc taattctttc gccacgcgaa tcgcacttgg cacacctgat
    30361 ttcaacgtat cataagtgtc gactaagaac acgcagtctt tatgggtttt agcgtaggcc
    30421 ataaatgctt cataatcatt tccataagat tggactaacg aatgagcatg ggtcccgcta
    30481 gctggtatcc caaatatctt cccagcacgt acattgcttg ttgcatctgc tccgccgata
    30541 tatgccgctc tagttcccca gactgctgca tccagttctt gtgcccgtct agttccaaat
    30601 tccaatagtg gatcttctcc gatgacagat ttgatacgag ctgcttttgt cgcgatcaac
    30661 gtttggaaat tgaccatatt caacagtgct gtttctacta attggcagtg cgctaaaggc
    30721 ccttccactt ggatcaatgg ttcatttgca aaaactagtt ctccttcacg agcagaacga
    30781 actgttgctt taaattcaaa attttgtaaa tactccaaaa aaccttcagg atagacttct
    30841 aatccacgca agtaagcaat atcggaatca ttgaacgtca gattctctaa ataattgact
    30901 aaccgttcca atcctgcaaa aaccgcgtat ccgtggttaa aaggcatttc tcgaaagtaa
    30961 cattcaaaaa cagcatgtaa atcagctcga cctaattccc agtatgtctg catcatattg
    31021 atttgatata gatccgtatg cagcgtcaaa ctgtcgtctg ggtaagtatt attcatcttg
    31081 tattactcct cttggcaagt tttcatttcc tacccattat aacaaaaaaa attaatagga
    31141 aaagaaatct tccatttttt ctgaaaaagc acatgtttta cggatatttc ttgctaaaaa
    31201 tgaactccat ctgctaaaac tatcctttct tattggtatt taacctaaac gagaaagaat
    31261 acttatttta ttcaatgtat ccgagacacg aagaaattag cgttttctat ttttcttact
    31321 tgataaaata agtagatttt cctactacaa tcgtagccgt taagataccg atgattgaac
    31381 aaatataacc aaatgtctct acaacataat caatggcaaa agtttttgaa gcaataatac
    31441 gtctttcctt ttttgcttct tatccccaaa gaaacggata taacttatta tgtattgaat
    31501 ttttttggaa aggcgaatta tgacaagata taccttgcgt tgaaaaaact cgcggaaaaa
    31561 atatcacacg tttttaaaaa gatacaaagt aagaaagcag ctgtcaatag aatggaaaca
    31621 aaaatggcga gacatcaatc agtctcgcct tgttttt
//
LOCUS       DAPCMU010000017        30489 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPCMU010000017 DAPCMU010000000
VERSION     DAPCMU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.017"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(40..285)
                     /locus_tag="RD744_000655"
     CDS             complement(40..285)
                     /locus_tag="RD744_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDU0556684.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(401..979)
                     /locus_tag="RD744_000656"
     CDS             complement(401..979)
                     /locus_tag="RD744_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0556685.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1056..1586)
                     /locus_tag="RD744_000657"
     CDS             complement(1056..1586)
                     /locus_tag="RD744_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDU0556686.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1590..2450)
                     /gene="rlmB"
                     /locus_tag="RD744_000658"
     CDS             complement(1590..2450)
                     /gene="rlmB"
                     /locus_tag="RD744_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDU0556687.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2443..2838)
                     /locus_tag="RD744_000659"
     CDS             complement(2443..2838)
                     /locus_tag="RD744_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDU0556688.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2835..4247)
                     /locus_tag="RD744_000660"
     CDS             complement(2835..4247)
                     /locus_tag="RD744_000660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDU0556689.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4244..4780)
                     /gene="cysE"
                     /locus_tag="RD744_000661"
     CDS             complement(4244..4780)
                     /gene="cysE"
                     /locus_tag="RD744_000661"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDU0556690.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4866..5105)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5102..6562)
                     /locus_tag="RD744_000662"
     CDS             complement(5102..6562)
                     /locus_tag="RD744_000662"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDU0556691.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6732..7889)
                     /locus_tag="RD744_000663"
     CDS             complement(6732..7889)
                     /locus_tag="RD744_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDU0556692.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7923..9293)
                     /gene="radA"
                     /locus_tag="RD744_000664"
     CDS             complement(7923..9293)
                     /gene="radA"
                     /locus_tag="RD744_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDU0556693.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9356..9883)
                     /locus_tag="RD744_000665"
     CDS             complement(9356..9883)
                     /locus_tag="RD744_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDU0556694.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10113..10919
                     /gene="proB"
                     /locus_tag="RD744_000666"
     CDS             10113..10919
                     /gene="proB"
                     /locus_tag="RD744_000666"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDU0556695.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10916..12163
                     /locus_tag="RD744_000667"
     CDS             10916..12163
                     /locus_tag="RD744_000667"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDU0556696.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12239..12583
                     /locus_tag="RD744_000668"
     CDS             12239..12583
                     /locus_tag="RD744_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556697.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12700..13026)
                     /locus_tag="RD744_000669"
     CDS             complement(12700..13026)
                     /locus_tag="RD744_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556698.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13641..14843)
                     /locus_tag="RD744_000670"
     CDS             complement(13641..14843)
                     /locus_tag="RD744_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0556699.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14960..15475
                     /locus_tag="RD744_000671"
     CDS             14960..15475
                     /locus_tag="RD744_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0556700.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15674..16120)
                     /locus_tag="RD744_000672"
     CDS             complement(15674..16120)
                     /locus_tag="RD744_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDU0556701.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(16167..17645)
                     /gene="msr(C)"
                     /locus_tag="RD744_000673"
     CDS             complement(16167..17645)
                     /gene="msr(C)"
                     /locus_tag="RD744_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDU0556702.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17999..18628)
                     /locus_tag="RD744_000674"
     CDS             complement(17999..18628)
                     /locus_tag="RD744_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDU0556703.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18612..19076)
                     /locus_tag="RD744_000675"
     CDS             complement(18612..19076)
                     /locus_tag="RD744_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0556704.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19563..20831)
                     /locus_tag="RD744_000676"
     CDS             complement(19563..20831)
                     /locus_tag="RD744_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDU0556705.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(21062..21799)
                     /locus_tag="RD744_000677"
     CDS             complement(21062..21799)
                     /locus_tag="RD744_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0556706.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21866..22435)
                     /locus_tag="RD744_000678"
     CDS             complement(21866..22435)
                     /locus_tag="RD744_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDU0556707.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22563..23435
                     /locus_tag="RD744_000679"
     CDS             22563..23435
                     /locus_tag="RD744_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0556708.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23777..24649
                     /locus_tag="RD744_000680"
     CDS             23777..24649
                     /locus_tag="RD744_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0556709.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24646..26604
                     /locus_tag="RD744_000681"
     CDS             24646..26604
                     /locus_tag="RD744_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0556710.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26936..27787)
                     /locus_tag="RD744_000682"
     CDS             complement(26936..27787)
                     /locus_tag="RD744_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0556711.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27910..28377
                     /locus_tag="RD744_000683"
     CDS             27910..28377
                     /locus_tag="RD744_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDU0556712.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28640..29122)
                     /locus_tag="RD744_000684"
     CDS             complement(28640..29122)
                     /locus_tag="RD744_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDU0556713.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(29222..30085)
                     /locus_tag="RD744_000685"
     CDS             complement(29222..30085)
                     /locus_tag="RD744_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDU0556714.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8718 a         6449 c         5513 g         9809 t
ORIGIN      
        1 aagcaaacga accttacgaa tctcaggctc gtttgctttt taaactgctt cagttaaaaa
       61 ttcgatctca actgtccgag taagtacatc tgaatagctg taagatactc gttcaaaaga
      121 attttcttca ggatccaaat caacaacgaa tactgaagga taggtttctg ttaaaacgcc
      181 gcggcgttct gtttgacgct ttcttcctgt ctgagcaacc aacatgattg ggctgccgat
      241 acgaccttct aaatctttct tgattgaagc taaagttgtt ggcatacagt tcacctctac
      301 tatcaactat accacatatt tttaaaattt tcaagtttac cataaaaaaa aaaagaaatg
      361 caataaaaat aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt
      421 tcaagtgttt ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat
      481 ccattgctaa acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa
      541 ttttagcttc tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa
      601 gatgctgaat tgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg
      661 tctctatttt ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct
      721 gcttcctcaa cagagaatag atttgattgc ttataacgct tttataataa cttccaaagc
      781 taacaccttt gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt
      841 gttcccaatc ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa
      901 gtggaaaata tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc
      961 gcaataactc ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat
     1021 catcaatgat tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg
     1081 tccaattttt gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa
     1141 tcaataaaat gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc
     1201 ttatataatt cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct
     1261 gctaaatcgc ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt
     1321 ctctcgatat aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat
     1381 ttcttataat tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc
     1441 attccttcat atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc
     1501 attttgtcct gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg
     1561 tctacaatca acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga
     1621 tacataagca aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg
     1681 atcgtcagca attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta
     1741 ccaatgacta aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata
     1801 tctgtgccaa atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc
     1861 gtcacgcgtg caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt
     1921 gtgattccaa ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct
     1981 gtccgtaaaa tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag
     2041 gggtcttctt ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa
     2101 ggtgtaatcg caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt
     2161 tgtttaggaa cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct
     2221 actttttctc ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc
     2281 gcttctactg cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga
     2341 tcagaaactg actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg
     2401 tcttcaaatc cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc
     2461 aactttctga atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg
     2521 cagatagccc atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt
     2581 ttttgcgctc gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc
     2641 tgtcaaaaga tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac
     2701 ataattcgtt gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg
     2761 gtcacgaaca tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa
     2821 ttgtgtgtaa tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat
     2881 aagatccctt tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg
     2941 cttcttgctt gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct
     3001 gctgtaaata aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc
     3061 aagacagcgg cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca
     3121 tagaccactg tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc
     3181 tgttctaaat tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca
     3241 gtttcttggc ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct
     3301 gcttctttca tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg
     3361 aagaaacgca agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc
     3421 cctagagatt tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc
     3481 cagtaattag cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga
     3541 tgaggaaatt ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt
     3601 gttgccataa cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc
     3661 caagaaatct cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt
     3721 tgctgctgtt cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg
     3781 tcacttaatt tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat
     3841 tcataagcat agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga
     3901 tccatgactc ttggatgcag tgtagcaggt tcgacattca acgctttcgt atcttcttca
     3961 aatgctcgga tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta
     4021 cggatgattt tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga
     4081 tattcaaaat agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg
     4141 atataattat acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg
     4201 atgaattctt ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca
     4261 ctttttctcc tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg
     4321 gaacatcttt caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg
     4381 gtcctaatat ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt
     4441 tacccgtttc ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct
     4501 cgatttctgc ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc
     4561 caatcgttgc tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga
     4621 tcttcgccaa taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca
     4681 aggcatgaaa accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt
     4741 ttctctttgc tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta
     4801 agaattttat gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt
     4861 ccttatcaaa agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct
     4921 gacagctaac gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct
     4981 gttttctgtg tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc
     5041 ttgcaccacc cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg
     5101 tttagttcag tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg
     5161 tttcaggcaa ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa
     5221 gattttttcc ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg
     5281 gagcatctac tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa
     5341 ctgtttcacc agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa
     5401 atagcgtaga cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa
     5461 cgatcttctt cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag
     5521 cgattagata aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat
     5581 ggttgtttac ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt
     5641 ttggatcgaa cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg
     5701 accagcccaa taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat
     5761 attgttcgat aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac
     5821 tgttgatgat caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga
     5881 tcatcagttg ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt
     5941 tcatcaaatg atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt
     6001 ggatgacaaa gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat
     6061 ctgtaaatgc gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt
     6121 gtgcttcttt ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag
     6181 gcatttctcc tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat
     6241 agcatttgta tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt
     6301 tacgttcaga ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat
     6361 tcattcccaa ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt
     6421 tttggtcagt atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat
     6481 aattgaataa tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat
     6541 agcgtacgcg tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag
     6601 caaaaattcc gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa
     6661 ttttaccttt tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct
     6721 gaatttcttt tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt
     6781 cctcgtcctg catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca
     6841 cttgttacaa cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc
     6901 atcgtgccgt catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact
     6961 agaacattca tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg
     7021 atattaagaa caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt
     7081 acaatgacac catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata
     7141 tcttcaaagt ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt
     7201 aggatatcta atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg
     7261 tattgtagtt cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc
     7321 tttgtaatat cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta
     7381 tggaaatttt ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct
     7441 tgctcgattt ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt
     7501 cccattctca atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg
     7561 acgaatggaa tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg
     7621 atgatcgcac cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt
     7681 tctacttgtt tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa
     7741 ataagtgcgc ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga
     7801 ccgattgcct gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc
     7861 atcagcaaag taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt
     7921 agttagcgga acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt
     7981 ccttttggcg cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg
     8041 agtttttgga cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc
     8101 agtccaattt caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa
     8161 gcaatactga cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca
     8221 gcttttaagt aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc
     8281 atgatcagag atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac
     8341 atagtaggtg tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc
     8401 acaacgatag cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga
     8461 ttagcgactt cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag
     8521 ccaaatcggt tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa
     8581 tataacaccg tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg
     8641 gtaacatgac cgacgataaa aatggctatc ccatttgttt tagcggtttt gagtagttct
     8701 gcagtggttt ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc
     8761 atcgtttgga tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg
     8821 atttcgttca tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt
     8881 tccgcgcgta attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg
     8941 cctatggctg ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga
     9001 tcgccaccaa tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt
     9061 tcttctaatt ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt
     9121 ggttttgctt tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg
     9181 atttcttcga ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc
     9241 gacacatacc cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg
     9301 cctcctttta tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt
     9361 tactggaaga accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta
     9421 aaaacggcaa aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga
     9481 aaccaaagtt ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat
     9541 cactgtcaat cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg
     9601 aacggttagc aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca
     9661 cgagggttgg tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga
     9721 ctgtttccaa cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg
     9781 ctgcttcaaa atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt
     9841 gatctttata tttagaaata acttcaaatc ctctttttct caccggtttt cctccctaaa
     9901 taaactactc ctattctaac atatccgagg ctgtaaaaga ctttctttac aaattcttca
     9961 agaaattctg ctatcttaaa gtaaaccatt aattcgttct ttttccaaac gactgcaagt
    10021 tgtacaaaaa aactattttt tacatgactt tactcgtcaa aacaagtaga atagaacgaa
    10081 attaatgagt ggatatttga ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac
    10141 gaattattgt caaagtagga acaagtacat tgatttatcc caatggaaat atcaatcttg
    10201 cggcaatcga tcagttagct tttgtccttt ctgatcttag aaatcgggga aaggaagtga
    10261 tcttagtctc atcaggagct gtaggcgttg gtatgcataa attacatttg tcagaacgtc
    10321 cgactttgat tcctgaacag caagctgcgg cggctgttgg tcaagccgaa ctgatgaata
    10381 tctataatca aagattcagt gtatataatc aacagacggc acagatcctc gtgacaagag
    10441 acgtggtaga ttatcctgaa agtcgtaaaa atgtgaggaa tacattggaa cagcttttga
    10501 ttatggacac gatccctatc gtgaacgaaa acgatactgt agcagtggat gaattagatc
    10561 atctgacaaa atttggcgat aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg
    10621 cagacctttt gatcgtcctt tctgatatcg acgggtttta ttcagataat ccgactacaa
    10681 atcccgaggc tcagatgtat cgctatatcg ataaaatcga tgaaaagatt ctggcacaag
    10741 caggtggtgc tggtagcaca tttggaacag gcggtatgca gagtaaatta aaagccgctt
    10801 ctaggatatt tgaagcaaac agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta
    10861 tttttgatat tcttgcagga aaagaagtag gtactttatt taaggaggac ttgaaatgac
    10921 tgacttgatg caattaggac aacaggcaaa agatagcgcg caacaactag cacttatgcc
    10981 tacaaaccgc aaaaatgagc tacttgttca aatggcacaa accatcaaaa agaatcaaca
    11041 agcgatcatt gaagcaaatc ataaagactt agaaaaagca gtcgaaaaca atatcagtga
    11101 aacgatgcaa gaccgcttgc agctgacacc tgagcgaatc aatgccatgg ctacagagat
    11161 cgaaaaaatt gcttcgttag acgatcctat tggaaaagta gatgagatgt ggacgaacac
    11221 cgacggattg agaatcggca aaaagcgcgt gccactaggc gtgatcggca tcatttacga
    11281 atctcgtcca aatgtcacga cagatgcagc tagtctagca ttcaaatcag ggaatgccgt
    11341 tatccttcgc ggtggcaaag aagcattctt ctcgaatcaa ttattagtcc aattattgca
    11401 gcaagtactg gtcagtgctg gcgaatctcc ttatgccatc cagtttgtcg atgatacttc
    11461 tcatgaaact gcaggacagt tgatgcgatt gacagaatac ttagatgtgc tgattcctcg
    11521 aggcggagca aaattaatcc aacgagtcaa agaacaggca acggtaccta tcattgaaac
    11581 aggtacagga aataatcacg tatatatcga taaagaagca cagttatcta tggcagtgaa
    11641 catcatcgtc aatgcaaaag cttcccgtcc atctgtctgt aacgcggcag aaacattgct
    11701 gatccattct gagatcgcac cttttttctt gccagctatt gaaaaagaac tggtagaaca
    11761 cggggtctct ttacgtgcgg atgctcgtgc gttagaatat ctagagacgg ctgctttagc
    11821 agaagacaca gattgggata ctgaatatct agattatatc cttgctgtca aagtggtcga
    11881 ttctttggat gaagcaattg cacatatcaa tcggcataat actaaacatt cagaaacgat
    11941 cgtgacagat agttatgcgg ctagtcaacg tttcttaaat gaagtggacg cggcggctgt
    12001 ctatgtcaat gcctccactc gttttacaga cggatcggta tttggttttg gtgcagagat
    12061 tggtatctct actcaaaagt tgcatgcccg tggtccaatg gggttaaatg aattaacttc
    12121 cacaaaatat atcatttatg gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat
    12181 tattcccgcc ttttgccttt tttctgtaaa ggttctatcc aactagaaag gtgtcaatat
    12241 gtttgaaatt ttaatgattt tttttattta ccttatttct ctgaatatcg ctgcttttct
    12301 gggagtcggt attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg
    12361 ggaaaaatgg tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca
    12421 cattagttac atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga
    12481 tcattcaatc gcttatacga ttacgctact gattgctggg atttttcact tatcttataa
    12541 atatcagctg aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata
    12601 agtagagtgc cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga
    12661 ttttgtcaca gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc
    12721 tgtttcctct tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag
    12781 cagctcatcc atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag
    12841 tagctgttcc cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt
    12901 ttcaccttcc gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc
    12961 atctgaaaat tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc
    13021 cagcatctat tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat
    13081 tttccagttt cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc
    13141 tttttccgaa taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac
    13201 aaacatgaga aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc
    13261 acaacctctt ggtaaacggc gttcaatcag ctgaccctcg gtattagctc aaaaaacggt
    13321 aaaatatgag aagctatttt tccgttttgt ttctctaata cttaggataa aacgctgatt
    13381 ttacaacctc tgttaaacgg tgttcaatca gctgtccttc ggtattagct caaaaaacgg
    13441 caaaatatga ggaactattt ttccgtttgt ttctctaata ctcaggacaa aacgctgatt
    13501 ttacaacctc ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa
    13561 acgaaaaata tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct
    13621 ttttcacaac ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa
    13681 atgatacaac atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca
    13741 ccaatatgtt caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg
    13801 acaaaactat agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc
    13861 gtcgtcaaca gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt
    13921 acaaggactg gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca
    13981 aatgctgcta aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa
    14041 atcgaagcaa tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca
    14101 gaatagccaa tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat
    14161 aacaatgcga ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc
    14221 caaaggccta cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata
    14281 aaaccgcaaa acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc
    14341 aagaccccac cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc
    14401 atgattgctt tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag
    14461 acaaataaag cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc
    14521 ccagataatc cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt
    14581 tttttcaaac cgatccgact agataagaaa cctgtaaata gcatcaccac accagtgact
    14641 aacatgtaac tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca
    14701 atcgttttta agattgggtc tactagaccc actcccataa aagcgatcat acaggtagct
    14761 gctacaacta gcgctgaact tttccccttt gtttcttcat ttgtttgttc ttgtatatct
    14821 atttgattct ccaatatttc catccctttc tctctattag catcatacat gatttatttt
    14881 acatgtcaat atgcacatgt aaaacgtgga aactttactg tgatataata aagaaaataa
    14941 gacgagaggt aggattttta tgaatacttt agcctatata ttattatgcg tgcttttatc
    15001 caaaccacgt tctgggtatg aattgaagca gctgatcaca attttttggg aagcccatca
    15061 cagtcagatc tatacaacgc ttgccaagtt agaccagcag ggttatgtga ccgttttgga
    15121 caaagacgag cactctcaaa aaaagatcta ccacttgact gatgaaggac gtgctcttgt
    15181 agaggaatgg atcaaagagg aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa
    15241 aatctatgct ttttcgacgc tcgataaaac aacagctact ggactgctat tcactagaga
    15301 acaacagctg aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga
    15361 caagaaacaa gaatttggac gttatgttgt gatccagcgc agaattatgc tttgtcagca
    15421 ggaactccaa tggtgtcagc aagtaagaga acaaatgcag cagttttttg agtaagggat
    15481 aaacagaacc tttctagaaa tatctattga agagcaaatt atcttcaata aaagagcaat
    15541 acagttggaa aagaaaaagc gatccgaacg acaggccaaa acctatcgtt cggatcacta
    15601 tttttctaga actgcattca agagactatg acaaaaagca tgccaaggcc ttttttcaaa
    15661 taacagtgtt ccatcattca ttactttgaa ttgcttttag cacacttctt gaaagcgggc
    15721 caacgatgat caactgcaac ggtaaagcgg caatcacatt cattttccaa gcattcatat
    15781 aagcagagaa taattgatca ggtattcctt gttccatgat cacaccgaat aaagacatac
    15841 atgtcaccat tcctaaaacc atcaagcatg aaatagttaa gatcatttgg accttgcttt
    15901 ctggatttac tggtagttta aaggcaattt ttttcgccag cacaccgatg atgaagacat
    15961 ccaaaatgaa agccaccacg aatccaggca ctagaccaat caacaaactt gataaatgaa
    16021 gatcttgatg cagccataaa ttatacgcgc tcatccccaa gaccatcaaa aaacacatca
    16081 ttgttgtaaa aattattcct tcttttttat ttgtaggcaa tctgtaccac tcctttaaat
    16141 ttgtcacgaa aatcatcgta gcacatttaa aaatttctcg taagtacttt ttttattttc
    16201 tgccctttta tttcataaac ttcatcagcc gtacgctcga caaagtaaga atcatgagaa
    16261 gtaaacaaaa tcgttcccgg atatatttgc attagcttct ctaaagcttc gatcgttgcc
    16321 atatcaataa aattagtcgg ttcatctaac agcaacacat tacttggctt agtaaaaagc
    16381 aaagcaatcg tcagacgggt cgcttctccc ccacttaatg tacataacgg tttcgtcaaa
    16441 gtttcctgag caaaccctaa gtgattcagc aaactacgga caagcgattc tgaaaactct
    16501 gtttgtctca tgacaaaacg aatgatggat tcttcagatg tcatttgata acccttttgc
    16561 tgatatactt gaaagcttac tttcggagag agaaggattc cttctccttg tttgcggata
    16621 tgttctaata aggttgtctt acctgaacca ttttcaccga caatcgcgat ttttttacct
    16681 aaagaaaact gaaaatcacc atttaccagt aatgttctcc cacttctttc caattggaca
    16741 ttttgtccca tgatgggata gcggctgtgg agttcaagag attttggtac tggaaaacgg
    16801 atttgtttca tctgctgcgg cttggttgtt tctcctattt tttggagtct tctctctaat
    16861 gttttcgcat tcttttggat ggctttttgt acactgtctt tttgtttaga ggaagacaac
    16921 cgatccagtc tgatttgttg ctttttcttt ttactggaca ctttttccaa cttttccgct
    16981 tgttttttct tttcttggat cgatttttcc aatcgttttt tctctttcat gatcgactct
    17041 gcttctcgta gctgagtctt tttctccaat tccttttgag tgagataggc actataattc
    17101 cccgaaaact ctcggatttc tccgtctttt acttcccaga ttttcgatgc tagctcatct
    17161 agaaaatagc gatcatgact aacaaaaacc agcgtaccat aataataacg aagctcttca
    17221 atcagtcgat caatactttt tctatctaga tgggttgttg gctcgtctag aagcatccct
    17281 tgttcataag aagaaagaag acgtatcaac tcgatctttc gttcttcccc tccgctccat
    17341 ttccgctctg aagtttcttc cagtcttaac tggaaatgac tggctaattc caaatttagg
    17401 tcctttgctt cagcgagata ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg
    17461 attttcccat gatcgggaaa aagttcaccg gctattagtt tgagcaaagt cgatttaccg
    17521 acaccatttt ctcctatgat ccctatacga tcttgctgat agacacgaag agaatcaata
    17581 gatagttcta gctggttgcc aaagctgact tgtagatttg ttatatttac tgctaaattt
    17641 tccatatgtt ttacactcct atttcatgta aaacaacaat tatcgatact aaaaaagagc
    17701 agaaaaatct gtttgcaaaa aaactgcatc acatcttttc ctactctgaa cgaaataact
    17761 atgactaaat caccaaatct gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg
    17821 gacatactcc atccatgaaa ttgttgttta tacatgtctt ttgaatttat ttagttgtta
    17881 ttcaaaagtt cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc cttttttatt
    17941 aggttaccac acacgattca aaaaaagcaa tagctactga atagtaactg aaaacctttt
    18001 attcgaacat agtgatcttt tctggtgcta aacgaacaga ctcgtaacct tcttctaccg
    18061 cttttcctat tgataaaata ataactggaa cataacggtc tttatccaaa ccaaatgcct
    18121 ctgccaactg atctgcttcg aatccaccaa ttggatttgt ttcatagcca tgatcacgcg
    18181 caactaacat gaattgcata gccgctaagc tagaatcgac tttgactaca tcgttcattt
    18241 cttctcttga aaaactcttg taataaggaa tgatcgcagc taattgttga tctctgactt
    18301 cttttggcat cttccctgat tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac
    18361 attgcatatc gccaaaaatc atgaccattg cagaagaagt atcattttgt ctcgtattga
    18421 aacggatcaa aggacgcaag atttcttttg cttcctcgct ttcagcgacc acaaagcgcc
    18481 atggtttcat attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt
    18541 cttcgtgtgg gattttgtat gtttcatcat atacgcgaac ggatcttcta ccagtcataa
    18601 ttgttgaaaa atcatttttt tgttccatta ttattaacct ctttcgtttc tgttgttata
    18661 ttgcaaagtt gggtattcaa tttatctaaa agtgttagaa attgtttcat ttctacatcg
    18721 ctgaaagccg gggatagcac ggaacctgta tctttatggt ccatttcaca atgcttgatt
    18781 ttcttctggg ctttttctgt tagcgctaca tacacttccc ggttgttctg aggatttctc
    18841 tgacgcgaaa catagccatt tgcttccaat accttcagat gacgagtaac tgctccttga
    18901 tcaatctgca acttttcttg caaactgctt tgtagcaatg gattttcttc tttcagtctc
    18961 atcatgatct cgtatctagt aagacttata ccgatatttt cttcaaagat ctggacgatt
    19021 ttttgatcgg ctaatttgat ttgatataaa aggtgactaa tctcttctaa agacataatt
    19081 tcctcctttc taataattga ctcgtcaacg attgacttgt caataataga ataaaaaaag
    19141 aaaaaagtca aagaatcagc tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag
    19201 aaaagaaatt ttttgtagaa tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc
    19261 aagattattg attcttatca aactatagaa gatagctctt ctgcagtaga taattttaca
    19321 ccgccaaaaa tttccgaaat cgccttttgc aagaatcccg tcgaatatta gaaattggat
    19381 cagtatgctt aaacagtaat aaaaaagtta tgacaagatg ttgtcacaac cttttttgag
    19441 taaacggtgt tcaaaaactt gcatctgcgg taataagttc aaccaaacaa aaaacatgaa
    19501 aatctgtttt tgtttggctg ttcttactac ttgatgcagg gcagcttttt cactacctct
    19561 ttttaataac cataaaatcc tattccttca tatctccagc ccatcggtgt tagccaatct
    19621 ctttccttag cactcaaggt atagtgatgg gtaccagttt tttcatttgg attaaatagt
    19681 cgataaattg gcttggaacc accagaataa aaacttagac cttcataacg ccaaccgatt
    19741 tttgttaaag agttgatctc agattgtgcc attgtgtaat gatggtctcc actgtaagga
    19801 ttgtataaac ggtagacagg ttgtccgctg tttggtgctt gccaagcaat cccttcatat
    19861 tcccagccta cagttggcag ataatttttt tctccggcat tcaatgtata taaatgttct
    19921 cctgtatgac ggttatacag acgatacatc ggagaagttg aagttggtgc tgtatcatac
    19981 tgtgtcagat tataggtttc gattacattg ttcaatgtag aaccataatt aggcgcagtg
    20041 gcataacgac ctgttagcca agccgtagca tctttgtaag agttcgtatt gcttttccat
    20101 gcaccggaat agtagtaaac gcctggctga aaagaagtcg tcttcaatac cgttacgtta
    20161 tcttgcaatg attctttgta cgaaggatat tttctaaaag cggcattcac cgtaatccat
    20221 tgcccattca catattccga agtaggcatc gtaacagatt ggccattata gcttccttta
    20281 atcccaaata gattgtaatt aggggcagaa gccaaagtac tgttccccca gccactttca
    20341 acgatggctt gagcaatcat gactgatgca tataagtcat tggcacttgc caaactttgt
    20401 gcagttgagg caatactatc aatgaacgct tgttgagaat tagcagatga gcgagcataa
    20461 aaagcatagc cagtatagtt ttcgaattca gacaaatctg cttgatgggt atgtgtcaat
    20521 gtttgcccat ctgtcaattg gttcgtgatt tcttctgcat tctctggatt gttctgagct
    20581 tctgctgatg tactgtcttc ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg
    20641 gttgaaattg tattttctga gacagtagct gactgcaaag tactttctgt atcgttttca
    20701 attcctgttt ctgaagaact cgatgtctga gttgcgacag tttctgtcga ttcttcagct
    20761 agcactgttg tgttggcaac tgctagattt cctaaaagaa taacgctaaa taataaattc
    20821 tttgctttca tcttttttct cctttgaatt atgaaacttt tcctaaataa cagtgtaact
    20881 aaaaaagtaa aagcaagtca atgtccatat ttaaataaca tttgtttctt tttaataaat
    20941 ttgctttttc tgcataaaaa gacgtaaatg aagaatattc ttctccattc acgccttaaa
    21001 ttgatggtcg tttgaataac tatttaacaa gtaattctct ctggatatta tccgttatgg
    21061 attatacttc ctgtgtcgtc ggacggataa tcatgttgct gacagacatc cggtctggcg
    21121 tttctatcgc aaatgctaca gcagaagcta tatcttcaga tgttaatccc cattcttttt
    21181 gcgcttcatg aagttcaaga gcagcctttt tgtcgctgat ggtttgatat aattcagttt
    21241 gaacagctcc tggagagatg atcgtagatt tgatgttatt ttgccgttgc tcttgacgta
    21301 atccttccat gattgcacgg acagcaaatt ttgttccgca ataaacggcg gaatcaggat
    21361 aaacaacatg tcccgccaca gaatcagttg aaatgatatg cccagatttt tgtttttcca
    21421 tgattggaag tacggcggaa attccattta acacacccat gatgttgata tcaagcatat
    21481 tttgccattc atcacggtga ccttcaacta atggagcggt tggcatgatt cctgcattat
    21541 tgtacaatac atcgattcga ccgaactttt ccatcgctaa atcgataact gcttggactt
    21601 gtgcaaaatc tcttacatct gctgtttgaa tatatagctc agcttctggt aaagattctt
    21661 ttattgcttt taggcgatct tctcggcgtg cagctataac tagtttagcc cctttttctg
    21721 ctaatagctt cgttgttgct tcaccgattc cgcttgaagc gcccatgatg acaatcactt
    21781 tttctgataa atcattcatt ctattctcct cctgttttga aaaatctctt gcttatactc
    21841 actatctctt atttcatctt actgttcaaa taatccgttc gacaggtttg acgattttgg
    21901 cagggattcc tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt gcattcgctg
    21961 caacaatggc attctcgcca atcgttacgc ctggcagaat agttgctcct gctccgatcc
    22021 aagcattttt cttgatgtgg attggcttta ctgacaagcc tcttcgttct tttggatcgg
    22081 ttagatggtt caccgtgatg aggttgacac gaggaccaat caatactgaa tcttctaaac
    22141 agatgccacc taagtcaacg aacatcactt gacgattgat aaagacattt ttccctatac
    22201 taatatgttt gccgaaatct gtataaaaag gcagcagaac agtcaccgta tcatctactt
    22261 tgtcactaat gatattttct agccgtttgc ggatatctgc tggttcatgg tacccagtat
    22321 tcagcgctgc taaaggctct tcgttttccg cttggatctg atggatcact tcaaagagcg
    22381 gatccgttac tcgaatctct ttattgacga tttgtttttc gatgaggctt gtcattttgc
    22441 tccctccttt tatccttact ttataacgga cggcttatga tgtctaatac ttataaaaca
    22501 tacttaacta ttccttttaa ctatacttag tggtatgctt ttttaaaaac aggagggaaa
    22561 aaatggatat tcgtatactg acgtattttt tagcagtagc tagagaaaaa aatatcagcc
    22621 gagcagcaaa atctctgcat atcacgcaac ctactctctc aaaacaactg aaagatttag
    22681 aagaagaact tggtgtgaca cttttcactc gaggctcccg agagatacag ttgacggatt
    22741 ctggtcgata tctttattca aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga
    22801 acaatttaac aaatgacgca atcatctctg gagaaatcta cattggcgga ggcgaaacta
    22861 aagcaatctc tttcatttct gatagccttc attcgttgat caaggcgcat cctgatatcc
    22921 agtttcattt atatagcggg aatgcagatg atatcataga caaactggat agcggattac
    22981 ttgattttgg actcgtaatc gaaccgacaa acaaacaaaa ataccagtca tttcgactgc
    23041 cagcccaaga tcgttgggga cttttgatac atcggaatca tcctttagca aagcaaacag
    23101 tcatccatcc gcaggaactg aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg
    23161 acagtcaact agctgaatgg ctaggtttca gcatagatca gttatcgatc gtcggaacat
    23221 ataacttatt gtacaatgct tctttgatgg tcgataacgg gacttttggt gcattagctc
    23281 tagatgggat catcaatact tatgggacgg aaaaagtatt catcccactc gatccgccat
    23341 tacaagcttc actgaatctc atttggaaaa aagaccagcc tctttctcct gctgctcgta
    23401 aatttttaga tgtcctaacg gagaaataca gttaagtaga gtttttttcc ttcgctttca
    23461 gctttttaac aacgaataag ttaagcaaaa gatccaatga ttaaataaca taagtttctt
    23521 aatttgttcc ttccttttat ttctgataat tccagtaaat tactataatt ataaaaaaag
    23581 aattgacaaa acataactgt ctatataaaa ttgattacgt taacaataat tcgtttcatg
    23641 tgactagatc atactaggca tgaaaaatct ctgaggaaca tggtttccct aggaggtttt
    23701 tcgtgtctat ttttgtttgg ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt
    23761 caaaaagggt gcgcgcatga aaatcaaaaa gatctttaat caaaatgcag tattggtaga
    23821 tgacggtgga caggaaaaag tagctattgg aaaagggatt ggatttgaca gaaaaagaaa
    23881 tgatttgatt tttgatcgtg atgtcgaacg gatatttgtc atggaaccag aaggtcaaat
    23941 caagttgcaa aatttactta atcagattga tgaacgcttt ttatttgcag cagaaacgat
    24001 cattgatcat gcggaaactg ttttgatgga aaagttgaat gaacatgtat tgatcgcttt
    24061 gaccgaccac attgcttttt ctgcggagaa cattaataac ggtatcgtca tccgcaacaa
    24121 acttttaaga gaaatcgaag tgctttacag tgatgaattc agtattgctc aatgggcagt
    24181 agagtatctg acaaaagagc ttgggattcc atatacttat gacgaagcag gatatatcgc
    24241 aatccatatt cacagcggac gaagcggtcg gaacaataac catcgcagta tccgtgaagt
    24301 gacgattgta tctgacatca tccatttagt ggaatcagaa ttggaaacag atatccattc
    24361 agaaccattt tcactgaatt attcaagact ggtgaatcat ttacgactgc ttctacaacg
    24421 aacccatgcg caacaatatg ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa
    24481 atacccagaa agctataaga tttctaaaaa aatccgtgtg ttgctgacca aagaatacca
    24541 gctcgcgatc acgaaagaag aactgggtta cttggccatc catatcgagc gtttaagaag
    24601 cgcaatcgta caaacaaatg ttaacaatca tgaggaggaa aaaaattgaa agcatatcta
    24661 caacgaatgg gacgttccct tcaacttcca gttgcggtct tacctgctgc tgctttacta
    24721 gtagggatcg gaaactggtg ggcatcttat agtaacgaca ttgtcgctca ttttttacaa
    24781 gctggcggta acgcagtttt aggtcaatta cctttgctgt ttgccgttgg gttagcacta
    24841 gggatgtcag tggacaaatc aggtgctgct gccttggcag gtttggtcgc atttgaagtt
    24901 ccagtaaatg ttttaaaaac tgattcagta gctactttat tgaatatcaa accagaatca
    24961 gttgatccat cttttgctca gatcagtaac gtatttattg gtattattgc cggattgatt
    25021 gcggcagctc tttataatcg tttccacgaa gcaaaattac cgatggcttt atctttcttt
    25081 agcggcaagc gtctagtgcc aatcttggct gcctttgtca tgttgctgat cagtgcagtg
    25141 ctactgctcg tatggcctcc ggtttactct gctttagttt cttttgctaa attcattttg
    25201 agtttaggct ggggtggtgc aggtctttat ggcttcttca accgtttact gatccctaca
    25261 ggattgcatc aagcaatgaa ctccgtgttc tggtttgacg tagcaggaat caatgatatt
    25321 ggtaaatttt tggcttctga aggaacaaaa ggcgtaacag gaatgtatca agctggattt
    25381 ttcccaatca tgatgttcgg gcttccagca ggagcatatg ctatttacag aaatgcacgt
    25441 ccagaaaaca aagcaaaaac agcttccttg atgttagcag cagcttttgc ttcattcttt
    25501 actggtgtaa cagaaccact tgaattctca tttatgttcg ttgcttggcc attatatgtc
    25561 ttgcatgcaa ttttcactgg gatttcttta gcagtgagtg cattcttcca ttggacagca
    25621 ggatttgcct ttagtgccgg atttgtcgat tacttcctat ctttgaaaaa tccgattgcg
    25681 aatcagccat tgatgttgat cgtccaagga ttagtaacag cagtgattta ctactttggc
    25741 tttaactttg ctatcaaaaa attccatctg atgactcctg gccgcgagga agcagatctt
    25801 tcagatgacg atactgcaac gactaattca gacaataaat atgcggcaca agcaagcaaa
    25861 atctacgctg cattaggcgg agccgacaat gtaacttcga tcgataactg tactacacgc
    25921 cttcgtttgc aagtcaaaga tacaggcctg atcgatcaaa ataaaatcaa agccactggc
    25981 gtacctggta tgaagatcat cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta
    26041 caattcgttg cagatgagat ggcaaaacta catggtggtg ctgcaacacg acctgcacaa
    26101 acaaacaccg tggttaaaac agaaacattt gccggtggaa cgatcgaaca agatatttac
    26161 gctatcgcaa atgggaaact gattccaatc actgaagttt cagatgatgt attcgcggaa
    26221 aaaatgatgg gtgacggcta tgctgtatta ccagaaaatg gagaaatctt ctctccaatc
    26281 gctggtacga tcacaaacat tttcccaacg aaacatgctg tcggaatcca aacagatgct
    26341 ggtatcgaag tcttgcttca tatgggcatc aacactgttg acttaaaagg cgaaccattc
    26401 actttatatg taaaagaagg tcaaaaagtc gcaagaggtc aattgattgc tttagttgat
    26461 cttgccgcta ttcaatctgc aggcaaaaac acggatatgg tcgttgtatt cacaaatggc
    26521 gacaaagtcc aaagcttaga aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc
    26581 ggctctgttt ccaacaaagc ctgagccatc gtcttcccca gactttttta tcgataaaca
    26641 aacaggagct gagacaaaaa tcttgtctca gctccttttt ccgaataaaa acggcgttct
    26701 cctgccaact ccgcggtaat aagtccagcc aaacgtaaca tatgaagagc tattttttat
    26761 ttgactcttc ttattacttg atgcagaaca gcttttttac aacctctgat aaacggtgtt
    26821 caaaaaccag tttatcggta tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca
    26881 caaaattttt cttcatactc aaaactaatc ggctttttca caacctcttt atacattatt
    26941 cctgatccaa tatttctttt aatgctgctt caagagaagt taatggtttt cccaatactt
    27001 tttctaaatc atcagaggaa atagctaact gatccttttt aatatcatgt tggatcgcta
    27061 aaaacatgtc agctgcttct tgagggaatc cggcttcttt tagattttcg acaaacgctg
    27121 catcacttgc ttcaataatt tctaactttt tacctgtcgc acgctctaat gccttggcta
    27181 attcttcata gtcagttaga ttgccggata attctaagat ttctggagag tcactttcta
    27241 ctagtacttt tgctgcagct tctgcatatt cacgtttcaa tgcccaacct gtttttccat
    27301 ttcctgcagc ataaacgaat tgaccagttt ttaaagcctg cccaatgata ggtaattcat
    27361 tctctacata ccaattattc cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt
    27421 tttcagtgaa ttggtgatct ggcgctaata cactgatcga gtgatcggca tctgcaaaac
    27481 ttgtataagc gatataagat accccagctg cttgtgctgc cttcactaca ttcttatgtt
    27541 cggcttgacg atttcctgga acgccagaga caaaaagcaa tcgatctatt cctttcaacg
    27601 cttctttcat tgagtctaaa tcatcataat caccaatacg tatattaaat ccagcttctt
    27661 tcagtgcttc tcctttttct tcactacgta ctagtacata gatttcattt tgtggaacca
    27721 tcttttttaa gaattccagc gcataaccac caaaacctcc agttgctcct gttactaaat
    27781 aatgcatatt tgccactccc tattcttttt atttgttaag atttacagct gtacatttat
    27841 aaattacacc tttatcataa agagtgtggt aagataagtc aagcaaaaaa aatgaaagga
    27901 tgacaactga tgaagtattc tctgcaattt agtgatgcaa tccatgtact tgcctatatt
    27961 gaaatgtttc aagatacccg atcactttct agtgagatga ttgccagtag tatcgaaaca
    28021 aatgcagcaa atgtacgtaa aatcatgagt cagctcaaaa aatccggttt gatctttaca
    28081 aaaaccggaa aaccgcagcc ttcccttgcc aaaagtccag atcagatttc tttactagat
    28141 gtctatcgca gcatcgaagg taatacacag cttatccaag tcgatccaaa aacaaatcca
    28201 aattgcgtcg ttggtgcgaa tatccaagaa gctttagaag aaagctacgc tcggctgcag
    28261 aaaaaggcag aagaagaaat ggctaagatc acacttgata gtctgataca taaaatagcc
    28321 gttttggaaa aagaaaaaag accagaaaac acaggcctcg tagagagatt tttataattc
    28381 tctctactgt gcctgcattt ttctgatcgg aatcgtctac ataaattctt ttattctccg
    28441 ggggacaaaa gccttgtcat gccccttttt cctctctaat gctcaggtca aagcgctgaa
    28501 gtcatgacgt cttgataaac ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa
    28561 tgcaaaatat ggaaagctat ttcccatttt tcctctctaa tgctcaggtc aaagcgctac
    28621 tttcacgacc tctatattat catggtttat tgactaccgg tatccagatt tctcccatat
    28681 atttcccgtt ggtaactccc atttcgacta cagcatttgg acccccaaca taggcgtaat
    28741 cttctacttc tcttaaaaca tgcccaaagg ccgtattcgt caaatcattt cttaattctt
    28801 caggcgattc tgctaaagcg attacaacaa gatacgttcc ttttgggaat gggatgattc
    28861 gggtagtgtt tggaacatcc tgactcgttt gaacaccgac atagtacatc attttcccgt
    28921 tatagggttc gttgactgta aagaaataat gattttcagc agcttgtttc aatgcatcta
    28981 ttgttccatt accgataact ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa
    29041 aatccgtatt gctactcttc aattccacac caatacccgt caccgtaaaa tctggttttt
    29101 cttcgatcct atactttgcc atttctccca ccctttctat tatattaagt tcatcttaca
    29161 taaaattaaa gggaaatgac acgattacgc tcttttttct agggatattt ttatttttta
    29221 actatttttt tgctttcact gcaatccaaa tctcactgta aacattactg aaatcctctc
    29281 cccattttgt aaaagagatc tcgggagctt cgactaactc ataatctgaa gaaggaagcc
    29341 attctgcata gattttcccc caagtatctt gtaaagtttg gggaaatggc cctttattag
    29401 gaaaaatggc ccaagtgtgt tcttctatag caagcatttc taaatcagcg tacggatttt
    29461 cttttgtcgt agcaaagcca atcatgtgca tcaaactgcc cttttcttcc aagcgggctc
    29521 catcaaatcc ataagaaacg ttgattactt gatttggata taaatctcct aacttatgca
    29581 tctcttccct ttgagtctcc gtgatcgtct gcgctaactt ttgtatttct tgattgactc
    29641 cttcaaattt gacaggaact tttttcgcca cacctgccac ataaaactgt tctttctttt
    29701 cgatacgaaa ttccatcgat tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa
    29761 aagatttttg tatgccgtat ctgctgattt ctgaaggcag atatcctgac caatcacgaa
    29821 aagctcttga aaaaccttcg actgtttcat atccatatct aaacgccgtt tctgtcactc
    29881 ttgatccatc taggagatcg cgattagcca aagccaatct acggtttttg atatagtcca
    29941 ttagcgacat ccctgcaata aacgtaaaga tgcgttttaa ttgatattca gagattcctg
    30001 tattttcggc tattatttta taggaaagtc cttctgtcaa atgttcttcg atgtaatcga
    30061 tcaattggtt cagatcttta agcatattta tcttcctcct tgcttgcctt tttatcatat
    30121 tatcaaagaa agtcaattac ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg
    30181 ttgtatggat ctaaagaaaa ttctcaagca gcgtttcgta acggataatt ggtattatct
    30241 gcctatctat gttttacctt tagaaaaata aattttgggg aagacacatt ctaagtaaac
    30301 aaagcggtaa aaatatcttt tattccctta gttaatccgc ttactaccta ctgtacaaag
    30361 caaattgttg ttcaaactgc tgatcagaac gctgcttgga tttttctttg cgatacatca
    30421 tacctctttt atcaattgga acaattattt atttctaaca aaatcacttc atttttgtca
    30481 gataaataa
//
LOCUS       DAPCMU010000018        30276 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPCMU010000018 DAPCMU010000000
VERSION     DAPCMU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.018"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            185..1123
                     /locus_tag="RD744_000686"
     CDS             185..1123
                     /locus_tag="RD744_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDU0556715.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            1146..1826
                     /locus_tag="RD744_000687"
     CDS             1146..1826
                     /locus_tag="RD744_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDU0556716.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            1799..2623
                     /locus_tag="RD744_000688"
     CDS             1799..2623
                     /locus_tag="RD744_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDU0556717.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            2790..3608
                     /gene="purR"
                     /locus_tag="RD744_000689"
     CDS             2790..3608
                     /gene="purR"
                     /locus_tag="RD744_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDU0556718.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            4111..5484
                     /gene="glmU"
                     /locus_tag="RD744_000690"
     CDS             4111..5484
                     /gene="glmU"
                     /locus_tag="RD744_000690"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDU0556719.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            5578..6549
                     /locus_tag="RD744_000691"
     CDS             5578..6549
                     /locus_tag="RD744_000691"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDU0556720.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      6555..6657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6723..7292
                     /gene="thiT"
                     /locus_tag="RD744_000692"
     CDS             6723..7292
                     /gene="thiT"
                     /locus_tag="RD744_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDU0556721.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(7373..8146)
                     /locus_tag="RD744_000693"
     CDS             complement(7373..8146)
                     /locus_tag="RD744_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556722.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            8413..10113
                     /locus_tag="RD744_000694"
     CDS             8413..10113
                     /locus_tag="RD744_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDU0556723.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            10083..10301
                     /locus_tag="RD744_000695"
     CDS             10083..10301
                     /locus_tag="RD744_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDU0556724.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            10321..11211
                     /locus_tag="RD744_000696"
     CDS             10321..11211
                     /locus_tag="RD744_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDU0556725.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            11229..11513
                     /locus_tag="RD744_000697"
     CDS             11229..11513
                     /locus_tag="RD744_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556726.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            12000..12614
                     /gene="gmk"
                     /locus_tag="RD744_000698"
     CDS             12000..12614
                     /gene="gmk"
                     /locus_tag="RD744_000698"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDU0556727.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            12619..12924
                     /locus_tag="RD744_000699"
     CDS             12619..12924
                     /locus_tag="RD744_000699"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDU0556728.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            13177..15585
                     /gene="priA"
                     /locus_tag="RD744_000700"
     CDS             13177..15585
                     /gene="priA"
                     /locus_tag="RD744_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDU0556729.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            15600..16091
                     /gene="def"
                     /locus_tag="RD744_000701"
     CDS             15600..16091
                     /gene="def"
                     /locus_tag="RD744_000701"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDU0556730.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            16084..17022
                     /locus_tag="RD744_000702"
     CDS             16084..17022
                     /locus_tag="RD744_000702"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDU0556731.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            17022..18380
                     /gene="rsmB"
                     /locus_tag="RD744_000703"
     CDS             17022..18380
                     /gene="rsmB"
                     /locus_tag="RD744_000703"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDU0556732.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            18393..19133
                     /locus_tag="RD744_000704"
     CDS             18393..19133
                     /locus_tag="RD744_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDU0556733.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            19130..21199
                     /gene="pknB"
                     /locus_tag="RD744_000705"
     CDS             19130..21199
                     /gene="pknB"
                     /locus_tag="RD744_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDU0556734.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            21364..22263
                     /gene="rsgA"
                     /locus_tag="RD744_000706"
     CDS             21364..22263
                     /gene="rsgA"
                     /locus_tag="RD744_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDU0556735.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            22276..22926
                     /locus_tag="RD744_000707"
     CDS             22276..22926
                     /locus_tag="RD744_000707"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDU0556736.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            22927..23565
                     /locus_tag="RD744_000708"
     CDS             22927..23565
                     /locus_tag="RD744_000708"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDU0556737.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            23720..24574
                     /gene="mreC"
                     /locus_tag="RD744_000709"
     CDS             23720..24574
                     /gene="mreC"
                     /locus_tag="RD744_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDU0556738.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            24578..25087
                     /gene="mreD"
                     /locus_tag="RD744_000710"
     CDS             24578..25087
                     /gene="mreD"
                     /locus_tag="RD744_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDU0556739.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            25342..26916
                     /locus_tag="RD744_000711"
     CDS             25342..26916
                     /locus_tag="RD744_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDU0556740.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(26984..28783)
                     /locus_tag="RD744_000712"
     CDS             complement(26984..28783)
                     /locus_tag="RD744_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDU0556741.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            28906..29412
                     /locus_tag="RD744_000713"
     CDS             28906..29412
                     /locus_tag="RD744_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDU0556742.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            29491..30213
                     /locus_tag="RD744_000714"
     CDS             29491..30213
                     /locus_tag="RD744_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0556743.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT         9996 a         5457 c         6491 g         8332 t
ORIGIN      
        1 tttttattgt agtgaaatgg ctagatatga gcagcaactc atccatttta cgatttaatc
       61 aagaatatac atgcttaaat cagaaattga tggatttcta ttgactgtgt ttttttattt
      121 tgatacatta gttcacgtta ataaatcgta aaattccgat ttgttcattg aaaggagcat
      181 ataaatgaaa aaatatcttt tgacagctac ggtaatgata ggtgctttac tgtttgcagc
      241 atgtggaaat acgaacaagg aagcagacaa aaaagaggat ttgacgatcg ttacgacttt
      301 ttatccgatg tatgatttta caaaagagat cgtaggggat gaaggaaacg tgaaactttt
      361 gatccctgct ggaacggagc cgcatgattt cgaaccaagt gcaaaagaaa gagcagaaat
      421 ttccgatgca gatgttttcg tgtataatag ttcagacatg gaattttttg tggattcgtt
      481 aaaggattca gtggatagca aacagacctt gatgatcgaa gcagctaaag gaatcgaccg
      541 tttagaatcg caagaagcag acgaacatga agaatcagaa gaagggcatg gtcattcaca
      601 tgaatacgat ccacatgttt ggctcgatcc ggtattagct ataaaagaag tcagaacgat
      661 tgctgaagaa ttaggcgaaa aatatccaga taaaaaggaa acttttacga agaatgccga
      721 tgcttatatc aaaaagcttg aagcactgga tcaaaaatac tccgatgagc taaaagatgc
      781 aacaaaccgc acatttgtca ctcagcatgc tgcttttgct tatttagcga atcaatacga
      841 cttaaaacaa gtagcgattt cgggcgtttc accagatcag gaacccacac caagtcgctt
      901 agctgagcta aaagaatttg tgaagaaaaa caacatcaaa gtgatttatt ttgaagaaaa
      961 tgcctcttcg aaggtagctg agacattgtc gaatgaaaca ggagtgaagc tagaagtgtt
     1021 gaatccttta gaaagcttga ctaatgaaca aatcaaagca ggcgaaaatt atatttctgt
     1081 catggaaaaa aatctgaaag ctttaaaaga aagtatcaat taagttttag aatggaggtt
     1141 gtaaggtgcg ttatatcgaa atatctgatt tgacctttta ctatgatgat gaacctgtac
     1201 tagaagatgt ctcttatcat gtagatgcgg gtgaatttgt cattttaact ggagaaaatg
     1261 gagcagccaa gtcaaccttg gtgaaagcat cgttaggatt actgaaacct tcaaaaggaa
     1321 tgataaagat tgctgagaaa aatcaagaag gaaaaaaatt aagtatcggt tatattcctc
     1381 aacaagttgc atctttcaat gcaggttttc ctagtacggt gatcgaatta gtccgttcag
     1441 gaagatatcc aagaggccgc tggttcaagc gtctctctga aaaagatcat caacatgtac
     1501 agcgtgcttt agaagctgta gggatgtggg atatgcgaaa tcggcgaata ggtgaattgt
     1561 ctggcggaca aaagcagcga atcagtttag cgcgtatttt tgctactgac cctgacttat
     1621 ttgttcttga tgaaccaaca acaggtatgg atgaagaatc cagaaatgaa ttttatcgtc
     1681 ttctccgaca tagtgcgcat gtccatggga aatcagttct aatgatcacc catgatcatg
     1741 aagatatcaa acagtatgcg gacagacaga tccgactggt gagaaaggag gattctcaat
     1801 ggcgttgctt tcatatgaat tcatgagaag agcgtttctt gccgctatct tcattgccgg
     1861 aatcgccccg atgctaggtg tgtttttagt gatcagaaga caatcgctga tggctgatac
     1921 gctttctcat gtctctttag caggggtagc gttaggattc tttttgaacc ttaatcctac
     1981 acttacgacc atgctagtcg ttgtattagc agctgtgata ttggagtatc tgcgaacgat
     2041 ctaccgaacc tattcagaaa tctccattgc cattttaatg gctggtggtt tggcgctagc
     2101 gttagtattg atgaatttga gcggagggaa ttcagcaaca agtatccaat cctatctgtt
     2161 tggttcgatc gtgaccatta catgggagca agtcatcttt ttaggaatct tattcgttgc
     2221 catcggtata ttgttctttt tattcaaacg gccgatgtat gttctgactt ttgatgaaga
     2281 taccgcccac gtggatgggc tacccgttca tttgatgtcg atgatgttca atgtgattac
     2341 tggtgtggct attgctgtga tgatcccgat tgccggtgca cttttgatat ccgcaatcat
     2401 ggttcttccc gcagcaatcg gtatgcgatt aggcaaaagc ttcaatgtcg tgattgctat
     2461 cagtgtttgt gtaggatttc tagggatgct gtcaggacta actagctcgt tttatctaga
     2521 tacacctcca ggagcaacaa tcacattagt ctttattgct ttgttcctga ttgtaaatat
     2581 catcaaacga atattggttg ccttcagaag aaaaaataac taaattataa agaaaagaag
     2641 ccttataaaa acgaaaaatt ttttataggc ttctttttat tttaataaat accgaactat
     2701 tttcttattt tttcataaaa tgctcgaatt tttgaagaaa aacttatata atgtaaaacg
     2761 ataaaatttc ttaaaaggag ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg
     2821 acatgacaca gtatttattg aatcacccgc atgaattgat ttccttaaca agctttgccg
     2881 aacgttatga atctgctaaa tcttctatca gtgaagattt gggaatcatc aagaaaacat
     2941 ttaaagaacg agggtatgga accttggaaa caatcccggg tgctgcaggt ggtgtattgt
     3001 tcatccctga aatttcatat gaagaggcga aaaaatatat tgaatctcta gcagaacgtc
     3061 tatcagaaca agaccgtctg cttccaggag gatatgttta tctctctgat ttgttaggag
     3121 aacctgaact gctgcgtcaa gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa
     3181 tcgatgctgt aatgactgta gccacaaaag gcgtacctat tgcccaagct gtgtcctatt
     3241 atctgaatgc gccattcgtt attgtacgcc gggactcaaa aataacagaa ggatcaacag
     3301 taagtgtcaa ctatgtgtct ggatcttctg aaagaattga aaagatggaa ctgtctaaga
     3361 gaagtttgaa acgtggttca cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa
     3421 cagtcaacgg aatgaagagc ttgattgaag aattcgaatc agaacttgtt ggcatcactg
     3481 tatttgccga atcaaaattc aacggtcatc gcgccatcga tgattatacg tcactgcttt
     3541 atgtcaaaga tgtagatact caaacgaaaa atattacggt tgtgccagga aattattttt
     3601 cagaataaga ggttgtgaaa aagccgctta gtttcgagta gtaagaaaaa ttttgtgaaa
     3661 atggctttcc acattttttc ataaatttgg cttaataccg agaaactggc ttttgaacac
     3721 cgtttatcta gaggttgtga aatcagcgtc ctgtcctgag tattagctca ataaacggaa
     3781 aaattgctcc tcatattttg ccgtttattg agctaatacc gaaggtcagc tgattgaaaa
     3841 acgtttaatg aagaggttgt aagcctaccg tttaactcta agtcacaaga aacgatttca
     3901 aaaaacagct tttcatgttt tatgagtagt gtgacttgtg accgaggagt tggcaggaga
     3961 gcaccggtta ccaaatagat aggcccgtta tggtctatct atttttttat ccagaaaacg
     4021 ctgggataaa agaaaatcac ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa
     4081 atcagcaaac tatatttagg agtgacgcag ttggacgcac gttatgcaat tattttggcg
     4141 gcagggaaag gtacccgcat gaagtcaaaa ttatataaag ttttacatcc cgtttctggt
     4201 aaacctatgg tagaacatat cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt
     4261 acgatcgttg gacacggagc agaacaagtt aaagcacaac taggagaacg cagtaaatat
     4321 gcgttacaag cagaacaatt gggaacagga catgctgttt tgcaagctgc ttctttcttg
     4381 gaaggaaaaa aaggaacaac gttagttatc agtggcgata caccattatt gactactgaa
     4441 acgctgaaca atctttttga atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg
     4501 gcacaagcag aaaacccaac aggatatgga cgaatcatcc gtgaccatat tgggatcgta
     4561 gaaaaaatcg tggaacaaaa agatgcaaca ccagaagaag ctcttgtaca agaaatcaat
     4621 acaggaactt actgttttga caacgaagct ttgttcgatg cgttaagcaa agttggaaca
     4681 aacaatgctc aaggtgaata ctatctaaca gatatcatcg aaatcttaaa agaagaaggc
     4741 catacagttg ctgcttatca aacggatgat tttgaagaat caatgggagt caatgatcgg
     4801 atcgcgttag ctaaagcaaa tgagatcatg agaaagcgaa ttaaccagat gcatatggtc
     4861 aatggcgtaa gctttgtcga ttcagcaacg acatacatcg atgcaggcgt agaaatcggt
     4921 ccagacacat tgatcgaagc aggtgtacaa atccaaggaa atacagtcat tggttctgat
     4981 tgtgtcatcg ggtcacattc aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt
     5041 gaaaactcag tcattgaaag cagccatgtc aagaaacatg cagatgtggg accttatgca
     5101 catttacgac caaaagctga aattggcgaa aacgtccata tcggaaactt tgtggaagtg
     5161 aaaaatgctc aaatcggcaa aggaactaaa gtcggtcact tgacttatgt tggtgatgct
     5221 acgctgggag aagaaatcaa tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag
     5281 aacaaacatc atacgactgt cggcgaccac agctttattg gttctagcac gaatatcatc
     5341 ggacctgtcg aggtagctaa aaacagttcg attgctgccg ggtcaacaat tacagacaat
     5401 attccagaat atgcgctggc aattgcgaga gcaagacaag tcaataaaga aggatatgca
     5461 aaaaaactcc cttatctaaa ctaagacttt tgcaaaatca cttgatttat ttaaaaaata
     5521 tgactactat ttattaggta gaattctaga gttgtgaaaa agaaagtgga ggtccccatg
     5581 tcaaaacatt actttgaccc aagattaaaa atttttgcgt taaattccaa tcgcccatta
     5641 gcagagaaaa ttgcagcagc agtcggcgtc gagttaggaa aatcatctgt tactcaattt
     5701 agtgatggag aaattcaagt aaacattgaa gaaagtattc gtggatcgca tgtctatgtt
     5761 attcaatcaa cgagcagccc agtcaatgat aacttgatgg agttgctgat catgattgac
     5821 gcactgaaac gagcaagtgc aaaaacaatc aacgtagtca tgccttatta tggctatgcg
     5881 cgtcaagacc gtaaagcacg tgcacgggaa ccaatcacag cgaaattagt agcaaacatg
     5941 attcaaaaag ctggtgcaac aagaatgctg actttggacc ttcatgcagt ccaaatccaa
     6001 ggtttctttg atattccagt cgatcatttg atgggtgcgc cacttattgc caattatttc
     6061 ttagaaaaag gaatcaaagg cgatgatgtt gtcgttgttt caccagacca tggtggtgtc
     6121 acacgggcac gtaaattagc ggaatttttg aaatctccga ttgcgatcat agacaaacgc
     6181 cgtccaaaag caaatgttgc agaagtaatg aatatcattg gacaagtcaa aggcaaaact
     6241 tgtgtactga tcgatgatat gatcgataca gcaggtacga tcacattggc tgccaatgct
     6301 ttgaaagaag ctggtgcaac tagtgtgtat gcttcatgta cacatccagt attatccggc
     6361 cctgctttac aaagaataga agattcagct atcgagcgtc tagttgttac agactcgatc
     6421 tatcttccag aagatcgtaa aatcgacaaa atcgatgaag taagtgttgg cggactaatg
     6481 ggagatgcaa tcaaacgtat ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa
     6541 aataaataat aaataaactc acaaagggga gtccaattgg gctgagattg aatctatttc
     6601 taaacccttc gtacctgtat cggttatgcg agcgtaggaa ttgtgaataa acagctgttt
     6661 gactattttc gaaagctgct tcctcctttg tgactttttt attcaaagga ggaagttttt
     6721 ttatgggaaa aaatagagtt tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt
     6781 tgtcgttgat tccgatccag atcggcagca gtttttctat ttcattaggg cagattccat
     6841 tgactctttt tgcgttaaga agagggacaa aggcagggat gctggcaggt ttcatttggg
     6901 gcatcctaca ttttccgttg ggacaagttt attatttaag tgtcgttcaa gtgttgattg
     6961 agtacccgat tgcctatact tttgcaggcg cagcagggct catggcttca aatgtccaaa
     7021 aaggctatgt agacgataat acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga
     7081 ttggcgcact ttccagatat ttttggcatt ttattgcagg agtggttttc tggggagcgt
     7141 atgcactatg ggggatgaat ccttggctct tttctttcgt aatgaatggt gccagcgggt
     7201 tgagtacagc tattgtaacg atcatcgttt tattagggat ccaacaaacg actccttcga
     7261 tttttgtgcc agataaacga caggagctgt aaataaaaaa gaagcaagaa acttgagaca
     7321 gaatcatgtc ctagtttctt gtttcctttt tattttttga taagaaatag atctacttca
     7381 atggcagata agaaagatcg gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt
     7441 agattaaagt agcgatgatt tcctcttctc gataccgggt gccttttaat ttccaaatat
     7501 aatcttcaat cacatagatt gggatacgat atttaccatc gatcaaacca acttgatcaa
     7561 aagaaaaaat gatcccttcc tgcatcaaat tacgcatttc tttcaataat tccacactga
     7621 ttttttcaac aggtgtttct tttttgaaaa aagatttttt tacattgatc ttatctgaca
     7681 agatcgaact taatttacta gcaaaaatcc tataaaattc atctaaatag cgataataaa
     7741 tccgattcag ctgatcatta agatcgaagt atacaaaatt attctgtagt ttataaaaaa
     7801 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
     7861 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
     7921 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
     7981 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
     8041 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
     8101 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
     8161 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
     8221 ttttaaaatt ccattacaaa atcacgtttt cattgaaaac actctcattt tcaattaatt
     8281 tttgtgattt ttgtattttt ttatttaaag gaggttttat gaattgtttt cttatggaat
     8341 ctacaaagtc gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa
     8401 aaggagagcg cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg
     8461 cctatccagc aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac
     8521 gctttttggc gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc
     8581 gtctatttta ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa
     8641 ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt
     8701 attaaagaaa actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt
     8761 tttcataaaa acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca
     8821 gcacttgttt taggtggagg aggagcgcac ggtgcttatc agattggtgt gtggcaggcg
     8881 ctgaaggaac atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac
     8941 ggcgcattga ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg
     9001 acaagacaag tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc
     9061 tatcgagaaa gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct
     9121 ccattggaaa agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa
     9181 atacgtttat atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc
     9241 caacagacaa aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc
     9301 gctatggctt atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa
     9361 atacctattg atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa
     9421 gggccaggcc cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa
     9481 acattgtgga cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta
     9541 caattaggct atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca
     9601 tttcattctg ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa
     9661 gaaattcagt tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat
     9721 ttatataaga acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca
     9781 aaaaaacatt ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc
     9841 tgccaacaag atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt
     9901 actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa
     9961 actgtttatt ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg
    10021 cttaagcaac cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa
    10081 ccatggcaca agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca
    10141 acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg
    10201 atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg
    10261 aagagtttga acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga
    10321 atgaaaagta tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat
    10381 attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc
    10441 ctgaactttg ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga
    10501 atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac
    10561 tgggaattga ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt
    10621 agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct tcgtgctgga cgaaaacttc
    10681 gtaacggtag aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat
    10741 acattgaaaa gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta
    10801 gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt
    10861 tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt
    10921 cttggagcga aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc
    10981 ggagatattc acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt
    11041 atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg
    11101 gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag
    11161 tctaagacga tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg
    11221 tagagataat gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta
    11281 aacgggatat cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact
    11341 ataatgagaa cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga
    11401 aatttattgt tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc
    11461 gaaaagatga gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat
    11521 gctgaacaaa gaaatacaat aaataaacag aattggattt cttcgcttag cttctttgct
    11581 ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat
    11641 aagtgatgtt caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa
    11701 actattttca caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta
    11761 tacacggcgt tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag
    11821 aagctgttta tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct
    11881 tggtacaggg tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg
    11941 cagaaaaaaa gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca
    12001 tgtcagagcg tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag
    12061 tacgaaaagc aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta
    12121 cccgtaaaat gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag
    12181 agtttgaatc aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt
    12241 attatggaac tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct
    12301 tggagattga agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca
    12361 tctttttgac tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag
    12421 atgcagatga agtaatcgaa aaacgaatga aagttgcaaa agaagaaatc gaaatgatgg
    12481 ctctttacga ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa
    12541 acattattgc cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt
    12601 taaaggagat gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta
    12661 aattcaaaat attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt
    12721 gcacagccta ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa
    12781 atcgatgcag gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag
    12841 atggaagaag aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt
    12901 atccgtgaag agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc
    12961 aaacccagct gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt
    13021 ctgaactttt tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt
    13081 ggcagaagat aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc
    13141 ttgataagaa aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt
    13201 gtggacgtac ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg
    13261 gaaacagctt tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat
    13321 gtccaaggat ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag
    13381 gcagttattc gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct
    13441 gattatatga aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg
    13501 agtgtgatga aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt
    13561 caggtattgt ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc
    13621 ctttctcaat tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat
    13681 acacgaaata aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc
    13741 gaagaagaat gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt
    13801 ttatctgaac tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact
    13861 gctgtcttga atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat
    13921 cgcgaccctt ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa
    13981 caaaaaaatg ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt
    14041 ttgctggagg gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat
    14101 gttttgtcag aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa
    14161 atggtcgagc gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt
    14221 tcccaaggtg aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta
    14281 gtaggcgcac gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat
    14341 gaagaacatg aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta
    14401 gcaatttggc gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct
    14461 ttagaatcaa gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga
    14521 gccaaagcag ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag
    14581 aatcatcgga cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac
    14641 aaaaaagaac aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt
    14701 agggactgtg gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg
    14761 gatacacgtt caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt
    14821 cctaattgtg gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag
    14881 gaactccgag aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga
    14941 agaaaagggg cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta
    15001 ttagggacac aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg
    15061 ttgaatgctg atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa
    15121 ttgcttaccc aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc
    15181 caaacattca atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag
    15241 ttttatcagc aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact
    15301 gtgaaaataa ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt
    15361 gccaatcagc taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc
    15421 atgattttga gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa
    15481 ccgaatcttc ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa
    15541 ggtctttttg tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta
    15601 tgcgctatcc tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg
    15661 atgtaatcac ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga
    15721 atgacggaat agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg
    15781 aagtagatga aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg
    15841 aaagccttga cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag
    15901 cagatgaagt gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt
    15961 ttggctattt agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca
    16021 ttgagaaaat gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc
    16081 ttgatgacta agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg
    16141 ctgatagaat caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg
    16201 cggaaacgag taattacgcc gacaccggta aaaaaagcgg ctctgaagca cggtatccgt
    16261 gtgctgcagc cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg
    16321 cctgatgtga tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta
    16381 ccaaaattag gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg
    16441 cctgttcatt atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg
    16501 atcaaaaaaa tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa
    16561 gatgatgtag ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa
    16621 acattgccca atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact
    16681 ttttcgccaa acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa
    16741 attgacaacc aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc
    16801 atacgctgga aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt
    16861 acgatcatcc ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta
    16921 gccatcaatg aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac
    16981 ggaagcggtc agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa
    17041 aaatgaaaaa atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg
    17101 gcgcctactc gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag
    17161 atgtgggttt atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat
    17221 tttacgttgc accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagatgcttc
    17281 tttatctttc tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa
    17341 acgaggcggt cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg
    17401 gtgtcttgag gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta
    17461 tcgaaagact ttctgtcaaa atcagcctgc ctctatggat gacgaaacga ctggtggatc
    17521 aaatcggata cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta
    17581 gtgcccgagt agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag
    17641 aagagatcac agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc
    17701 atttagcttc tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga
    17761 tgcttgtagc gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag
    17821 cacccggagg taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag
    17881 tggtcgcttt agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc
    17941 taggtgtctc ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt
    18001 tcgaagcaga gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc
    18061 ttcggcgaaa accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa
    18121 aaatccaatt ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga
    18181 cttatagcac ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg
    18241 aaagacaccc tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa
    18301 tcgaagagaa aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt
    18361 gttgtttgcg caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag
    18421 gaagacgacg taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca
    18481 agcttgctgt attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga
    18541 tggcggtaac gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa
    18601 tcgcccaatg gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac
    18661 aagaacagcc ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg
    18721 actggttcac gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa
    18781 tcatgcaatt gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca
    18841 gcgaagaaat ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc
    18901 ctggaacagt cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt
    18961 tatgttcaga tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc
    19021 aagaaggccc attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg
    19081 gagcggataa tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt
    19141 gggcaaaaaa ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc
    19201 aaatgtattt ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg
    19261 atttgacttt caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc
    19321 aacagaactc gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat
    19381 gcagtatctg gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca
    19441 ttatcctgtt ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc
    19501 tttagctcat aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga
    19561 taatgaaggt gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc
    19621 gatcacgcag acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag
    19681 aggaagtatg gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat
    19741 gttgacgggt agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt
    19801 tcaagatgat cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa
    19861 cgtgatttta cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat
    19921 gtctgatgat ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc
    19981 tcatgtgatg gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc
    20041 tgattcattc aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga
    20101 ggaggaagaa gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct
    20161 attagctatt cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg
    20221 aggagaagtt gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact
    20281 cgcaaaagcg aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga
    20341 agaaggtaat gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga
    20401 agtcactctg tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa
    20461 aagttatgaa gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca
    20521 aaaagaagag gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc
    20581 agaagataca gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc
    20641 agaaacagtg actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct
    20701 tgttacacaa ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt
    20761 accatcaggt tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac
    20821 ttacatcaat tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta
    20881 tacaaaaagc gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga
    20941 gacttccgaa tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc
    21001 aggaacgaca attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc
    21061 agcgtcttct agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag
    21121 tagcacaagc aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac
    21181 ctcagaaagt tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgatt
    21241 tgttgcattc tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt
    21301 gagaagtttg catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg
    21361 gaggtggcac ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac
    21421 gcaaacggag agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg
    21481 ccgcttgtcg gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg
    21541 gaaatcttgc cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga
    21601 atcattgtca caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta
    21661 gttaccttgg aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca
    21721 gacgacccag aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg
    21781 attgtaccca atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta
    21841 tcggtcttta tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg
    21901 gaactgaatt tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca
    21961 cgtcatgtgg aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc
    22021 tcgattgact ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta
    22081 gaagcttctg tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa
    22141 gtaaaacata gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa
    22201 ttcttacaag aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa
    22261 taggaggaaa caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta
    22321 caaagagaca ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg
    22381 gatggccgat ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct
    22441 gttactaaat taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca
    22501 gcatttgcaa aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt
    22561 catcgggcac ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca
    22621 ggaacaccag tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg
    22681 actgtcaatc ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg
    22741 gaactcgctg atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga
    22801 atcgttccag aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga
    22861 tcatatatct atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta
    22921 gactaaatga aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg
    22981 ctgtccaaat acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca
    23041 ggctacccag tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag
    23101 gaagtatttg caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat
    23161 acacagttag gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt
    23221 ggagctacgg gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct
    23281 cgcttcaaac cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat
    23341 tatttgcctg gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc
    23401 tgcttgactc ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa
    23461 gaagtagaat atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt
    23521 tctttttcag atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa
    23581 aaaaatttgt ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa
    23641 gaggagcaac ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta
    23701 attaattcga ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt
    23761 ggttattgtt atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga
    23821 aggaaagccc aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac
    23881 aatctctgcc ccagtacggt gggtacgaaa tggagtagat tctgtccata atcttttttc
    23941 aacttatgcc gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca
    24001 gcaaaacaag aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca
    24061 gacgctgact agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg
    24121 gcaggacatg ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt
    24181 catgtcgcaa aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat
    24241 cgaattactg tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc
    24301 taatggcgaa gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt
    24361 gacccaatta actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct
    24421 tggagggaat tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag
    24481 ttatgggctg gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt
    24541 tgtgacgatt gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa
    24601 gtactatctg ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat
    24661 gctaggggag tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt
    24721 attatgttgc agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg
    24781 ggctatctat gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt
    24841 aattgtatgg ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt
    24901 tttcagtatg atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat
    24961 ttttaaatta gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact
    25021 gctgttgaac atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga
    25081 tgaataattt ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag
    25141 aaatgctaga cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat
    25201 ttttaaaagt tgttttgaaa catttttgta acataactat tatatcgttg acacactgct
    25261 atggtacaat ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta
    25321 agccagtcgg aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca
    25381 atgaccttga ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag
    25441 atacaacagc aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca
    25501 caaatcgatg ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta
    25561 gcaaaacaag atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta
    25621 caagaacgta ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta
    25681 agcaacacaa gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct
    25741 attggacgcg ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa
    25801 caaaaacaag ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa
    25861 gaattagctg aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa
    25921 gctgatttga acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag
    25981 aaagctgact tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa
    26041 caacaacgtc tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa
    26101 gcacgcgaac aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc
    26161 tcagcaacag aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct
    26221 acacaatctt cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca
    26281 gcgcctgaat cttcagcaac agaagaatca acaacagcgc ctgaatcttc agcaacagaa
    26341 gaatcaacaa cagtgcctga atcttcagca acagaagaat caacaacagt gcctgaatct
    26401 tcaacaacag aagaatcaac aacacctgcg cctacaacac catcaacaga tcaaagtgtt
    26461 gatacaggaa acggcacagg aagtagtact ccggctccaa cgccaacacc aacacctgaa
    26521 caaccaaaac ctgtaacacc tgctccagct ccttctggaa gtgtgaatgg tgcagcaatc
    26581 gtagcagaag cttacaaata tattggtact ccatatgttt ggggcggtaa agatccaagt
    26641 ggctttgact gctcagggtt cacacgctat gtttacatgc aagtaactgg tcgtgacatc
    26701 ggtggttgga cagttcctca agaatcagct ggaacaaaaa tttcagtttc tcaagcaaaa
    26761 gctggagact tgttattctg ggggtcacaa ggcggaactt accacgtagc gattgcttta
    26821 ggcggaggac aatatatcca tgctcctcaa ccaggcgaaa gcgtaaaagt tggttcagtt
    26881 caatggtttg cccctgactt tgctgtcagc atgtaagaat gattaatcaa aaagagttag
    26941 cccattctgg actaactctt tttttattgt gataaaatag aaatcagata aattcttctg
    27001 tcaattccat gaacgtttgg atatcttcag tcaccatgtc gatacctgct tgccaaaaat
    27061 ctggttgggt aagatcgaca ttcaaatgtt tcttcgcaag atcttccgtc gtcattgagg
    27121 cagtatcgcg tagtaaatgg atatattctt cttcaaaatt tgctcccttt tggttggcgt
    27181 aagcatagat gcccaaacta aagaggtagc caaatgtgta tgggaagtta taaaacggta
    27241 cgtcatcaat aaagaaatgc aattttgcag cccagaaata tggatggtaa gtagctaaag
    27301 ctccttgata gccttctttt tgcgcctcaa ccatcatctc tgtgatctta tcttctgaga
    27361 ctaatccttc ttgccgtgct tcatagaatc gattttcaaa aatgaatcgg gaatggatat
    27421 tcatgaacat agctaaagca ttttgcagtt ttgtatctag caaattgatt ttttcttctt
    27481 ttgtttttgc gacttttaat gtagcatcgg ctacgatcag ttctgcgaat gtacttgctg
    27541 tttctgcgac attcatggca tattcgcgat ttaaagaagg taaatcccac atcgtgctgc
    27601 tatggaaagc atgtcctagt tcatgagcaa gtgtcgcaac ctcattaaca gaattactgt
    27661 aggtcataaa gatccttgat tcttttgttt ctggtaactc agtacaataa ccaccaggac
    27721 gtttcccagg acgatcttct gcttcgatcc agctcttttc aaaagcagat tgggcaaatt
    27781 tagccatttt tggactgaat ttgttaaagt tttcgataat aaatgctgct gcttcgtcaa
    27841 aactaaaggt tttttctttc aaatcgccta aaataatagg tgcatcttga tcttgccatt
    27901 ccattttttc tttaccgaaa agattagctt ttctagtcaa gtaattaaca aggggttgtt
    27961 tgtttttctg gattgtatcc cacatgacag acaatgtttc tttcttcaac cgattgtact
    28021 ctaatggttt ttgtagaaaa tctgtgacac catgaagttc atagtcagaa agtctaaaac
    28081 catctaaatg attcaaagta tccgtgaaga ggggtgcctt ttctttccag gcttcttccc
    28141 atgctgcgaa aagtgtttct ctgacttttg gatctggatc gcccatcatc ttattgaagg
    28201 cttgtccagc agataattcg actacttgtc catcttgttc gaagggaatc gaaatgctgg
    28261 ctacaatcgt atcataatga ctgctccaag catttagacc atctaaagaa agcgtattta
    28321 taatgttttc ttcagcttct gataataatt gtgagccatc acgacgaatc tcgtttaaac
    28381 gaaaagcaat tgtttcaaac gaagattgag aaagcatctg attccaatca ttgtcagata
    28441 tttctgcaaa tttctttgac aatacagttt cggctgattg cagacgaggc agcattgcgt
    28501 aaagcttgcc tgataagacc tttgcatctg aatcattgac attggcagaa agcaatgcag
    28561 taatatagct gctgcattgg gtaaaaccat tcgtaatcgt ttcttgcaat tgaagaatag
    28621 cgtttaactg ctctgcattg tcagaactag gtgaccattt tgttacgcgg tgataatact
    28681 cgttcatttg gtcttccaat tgcttcatcc gttggttcaa agcatcagaa tgactgcctc
    28741 cggaaaaaat agaatctaga ttccaattta atgaataact catgaaaaaa ctcccttcat
    28801 attgtaatac aaaattaagt atagcacttt tttatggaag aagtctctat gaaaatgatt
    28861 gttgtttctt caaaaaaagt ctaagataaa atacagaggt gacttatgaa ttcttcaaaa
    28921 aaacaaatga aaaacggaaa tatttattta gtaatcgcac tgatagtcgt tataatctta
    28981 ttttacagtt cttctcaaac ttacggacag caatcacaag tttcgcgatt agaaagctgg
    29041 ctgcctaatc agccatttaa agacacatta gcgggtatac gttttaatta tggtggaagt
    29101 atggtgagta tcaaccatct gggctacgcc aaatttttag agttcttttt aaggaagggc
    29161 gcacatttcg gtacctattt tattttggga ggcagcttgt ttttaggtgt tttccccaag
    29221 ctaaaaattt ggtggctgac agcgatcctt gcttggcttt cagcaacagg gtacgcaggt
    29281 ttggacgaat tccatcagat gatgaccggt ggacgaacac ctatgttcca agatgtcatg
    29341 ttggattcga tcggtgctct gactgctgtg atattatgtg tgctttttgc ggcattcaag
    29401 aagcgtttat agattggctg tggctttgtt tttttgtgaa atagtactag aatagaagtg
    29461 tattctcaaa agaacaaggg aggaaaaatc atgtcaggac atagtaaatg gcataatatc
    29521 caaggaagaa aaaatgccca agatgcaaaa cgagggaaag tctttcagaa actttctcgt
    29581 gaaatttata tggcagctaa agcaggcggt cctgatccat ctatgaatcc agctttgcgg
    29641 cttgttgtgg acaaagcaaa agctgcaaat atgcctaacg acaatgtgga acgggcgatt
    29701 aaaaaggcta cgagtgccgt agatgaaacg aattacgatg aaatcacgta tgaaggatat
    29761 ggtccgggtg gagtaggaat cttagtccat gcattgacag ataaccgtaa tcggacagcg
    29821 acaaatgtcc gagtagcttt tactcgaaac ggtggatcac tgggagaaac gggttcagtc
    29881 agttacttgt ttgaccgcaa aggatatatc gctattgaac gcgatgggtt gactgttgat
    29941 gaagagacga tgtttgagga tgtattagag gcgggggcag aagatctgca aacttctcct
    30001 gaagtattcg agatttatac tgcaccagaa gattttgctg ctgtacgaga tgagttggaa
    30061 aaagagttca ctttggcgca agcagaattg acgatgattc cacaaacgac gattgattta
    30121 aatgaggaac aaaaagagca attggaacgt ctagtagata agctagaaga cgatgatgat
    30181 gtttcagaag tctttactgc tgctgaaatg taagtagcac attgttagat aataaaaaga
    30241 aagcacctgt accaatcgta cgggtgcttt cttttt
//
LOCUS       DAPCMU010000019        30146 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPCMU010000019 DAPCMU010000000
VERSION     DAPCMU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.019"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(377..2104)
                     /locus_tag="RD744_000715"
     CDS             complement(377..2104)
                     /locus_tag="RD744_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDU0556744.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2097..3290)
                     /locus_tag="RD744_000716"
     CDS             complement(2097..3290)
                     /locus_tag="RD744_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDU0556745.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3477..4121)
                     /locus_tag="RD744_000717"
     CDS             complement(3477..4121)
                     /locus_tag="RD744_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0556746.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4215..4349)
                     /locus_tag="RD744_000718"
     CDS             complement(4215..4349)
                     /locus_tag="RD744_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDU0556747.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4351..6483)
                     /gene="feoB"
                     /locus_tag="RD744_000719"
     CDS             complement(4351..6483)
                     /gene="feoB"
                     /locus_tag="RD744_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDU0556748.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6480..6953)
                     /locus_tag="RD744_000720"
     CDS             complement(6480..6953)
                     /locus_tag="RD744_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDU0556749.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7354..7578
                     /gene="nrdH"
                     /locus_tag="RD744_000721"
     CDS             7354..7578
                     /gene="nrdH"
                     /locus_tag="RD744_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDU0556750.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7737..9896
                     /gene="nrdE"
                     /locus_tag="RD744_000722"
     CDS             7737..9896
                     /gene="nrdE"
                     /locus_tag="RD744_000722"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDU0556751.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9946..10911
                     /gene="nrdF"
                     /locus_tag="RD744_000723"
     CDS             9946..10911
                     /gene="nrdF"
                     /locus_tag="RD744_000723"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDU0556752.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11000..12139)
                     /locus_tag="RD744_000724"
     CDS             complement(11000..12139)
                     /locus_tag="RD744_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0556753.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12448..13161)
                     /locus_tag="RD744_000725"
     CDS             complement(12448..13161)
                     /locus_tag="RD744_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDU0556754.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13415..14116)
                     /gene="nagB"
                     /locus_tag="RD744_000726"
     CDS             complement(13415..14116)
                     /gene="nagB"
                     /locus_tag="RD744_000726"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDU0556755.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14350..15882
                     /locus_tag="RD744_000727"
     CDS             14350..15882
                     /locus_tag="RD744_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0556756.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16073..17275)
                     /locus_tag="RD744_000728"
     CDS             complement(16073..17275)
                     /locus_tag="RD744_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0556757.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17357..18046)
                     /locus_tag="RD744_000729"
     CDS             complement(17357..18046)
                     /locus_tag="RD744_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDU0556758.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18152..18520)
                     /gene="rplL"
                     /locus_tag="RD744_000730"
     CDS             complement(18152..18520)
                     /gene="rplL"
                     /locus_tag="RD744_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDU0556759.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18573..19076)
                     /locus_tag="RD744_000731"
     CDS             complement(18573..19076)
                     /locus_tag="RD744_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDU0556760.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19280..19407)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19486..20175)
                     /gene="rplA"
                     /locus_tag="RD744_000732"
     CDS             complement(19486..20175)
                     /gene="rplA"
                     /locus_tag="RD744_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDU0556761.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20279..20701)
                     /gene="rplK"
                     /locus_tag="RD744_000733"
     CDS             complement(20279..20701)
                     /gene="rplK"
                     /locus_tag="RD744_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDU0556762.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21044..21712
                     /gene="sdaAB"
                     /locus_tag="RD744_000734"
     CDS             21044..21712
                     /gene="sdaAB"
                     /locus_tag="RD744_000734"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDU0556763.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21725..22597
                     /gene="sdaAA"
                     /locus_tag="RD744_000735"
     CDS             21725..22597
                     /gene="sdaAA"
                     /locus_tag="RD744_000735"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDU0556764.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22688..23707)
                     /locus_tag="RD744_000736"
     CDS             complement(22688..23707)
                     /locus_tag="RD744_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDU0556765.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23880..24785)
                     /locus_tag="RD744_000737"
     CDS             complement(23880..24785)
                     /locus_tag="RD744_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0556766.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24844..25389)
                     /gene="nusG"
                     /locus_tag="RD744_000738"
     CDS             complement(24844..25389)
                     /gene="nusG"
                     /locus_tag="RD744_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDU0556767.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25490..25660)
                     /gene="secE"
                     /locus_tag="RD744_000739"
     CDS             complement(25490..25660)
                     /gene="secE"
                     /locus_tag="RD744_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDU0556768.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25684..25836)
                     /gene="rpmG"
                     /locus_tag="RD744_000740"
     CDS             complement(25684..25836)
                     /gene="rpmG"
                     /locus_tag="RD744_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDU0556769.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25998..26642
                     /locus_tag="RD744_000741"
     CDS             25998..26642
                     /locus_tag="RD744_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0556770.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26698..27201)
                     /locus_tag="RD744_000742"
     CDS             complement(26698..27201)
                     /locus_tag="RD744_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDU0556771.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27214..27260)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27798..28763
                     /locus_tag="RD744_000743"
     CDS             27798..28763
                     /locus_tag="RD744_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDU0556772.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28862..30034)
                     /locus_tag="RD744_000744"
     CDS             complement(28862..30034)
                     /locus_tag="RD744_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556773.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30021..>30146)
                     /locus_tag="RD744_000745"
     CDS             complement(30021..>30146)
                     /locus_tag="RD744_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556774.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9192 a         6195 c         5366 g         9393 t
ORIGIN      
        1 attaatgttt ttagactatt attctaacat tcattaaatt tcaaaaaaca attccttctt
       61 gaaacgtagc aaaataattc ctgaataaga atttaatcac ttttaagtat ataaactatg
      121 tatattcttt aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag
      181 tctttcatac ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg
      241 gcgtctttgg acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat
      301 agagacgaac atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta
      361 tttcgtgtct gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc
      421 agtcttttgc tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca
      481 tatctttttc tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt
      541 tcaatccttt tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat
      601 cttctaagta tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta
      661 caattggttc gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag
      721 atgtctctac tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg
      781 taactacacc agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact
      841 catcgatcga atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca
      901 aattggcacc taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt
      961 taggtagcca agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga
     1021 ttgaattatc taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga
     1081 ctacatttgt tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg
     1141 tttctttggc agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag
     1201 caccaatgat cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt
     1261 tattgtttgg ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta
     1321 atgcaacccc gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc
     1381 cgcgtcctaa gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca
     1441 ttgttgtttg attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga
     1501 atagtttttg acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa
     1561 cttgtcgaat ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg
     1621 caaatacccc tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga
     1681 atcctggcat tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc
     1741 tttttgccat caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa
     1801 gaacaagtgt gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata
     1861 gaccgattaa tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta
     1921 ccgtcaataa caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg
     1981 acatcactgc ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa
     2041 accattctgt gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat
     2101 tcatgttcac tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg
     2161 acacctttta attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata
     2221 ttgaaaatat cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag
     2281 tcgatcaatg gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct
     2341 ttcaggtgtc tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt
     2401 gtcaatgcaa cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg
     2461 ttttgagccg taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca
     2521 tttgctggat tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc
     2581 atcaaggcga ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata
     2641 atcgtttttt gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc
     2701 agcggatcaa gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa
     2761 gctctggcta ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct
     2821 ttaaatgaaa gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct
     2881 ttaggtacac cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga
     2941 aacagaccga agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct
     3001 tctttgctta acttcgatac atcttctcca tcaatataga tactgcccga agttggctca
     3061 atcagacgat tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc
     3121 acgaagattt ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg
     3181 gttttcttta aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt
     3241 gtctttttac caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc
     3301 cttttttatg taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat
     3361 gtagtcactt tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta
     3421 aatctttgta aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat
     3481 tctgcaagct gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt
     3541 ccaataaaat gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc
     3601 agttcttcat tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac
     3661 tcgctaattg aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt
     3721 aaaggattca tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa
     3781 aaatgattca gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct
     3841 tggatggatt gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca
     3901 acaaatgccc cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata
     3961 gccttgcggg tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg
     4021 atcttctcac ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc
     4081 tgctggtagc gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt
     4141 tttactttcc ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct
     4201 cttgcaacga ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag
     4261 tcatcacaat tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag
     4321 attaacacac ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt
     4381 ataaaactgg ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag
     4441 taaaatagca agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg
     4501 ataaatcaca aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt
     4561 ccatttcttg gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca
     4621 aagtaaattg aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac
     4681 ttgccagatt tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt
     4741 gccaataata ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt
     4801 tccccaacca agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact
     4861 ttggtcttca gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat
     4921 actagaaaca aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt
     4981 ttgtcttagg accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg
     5041 agctgtatct ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc
     5101 agtcatacca agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc
     5161 tgctactaag gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat
     5221 cgtcaaacga cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc
     5281 acatcctgta gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa
     5341 aagtcgatcc atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa
     5401 gcataaaaac aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat
     5461 gatgccgttc aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct
     5521 gccaacatgt tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat
     5581 tgttcctact gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg
     5641 caaggctaac caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa
     5701 agaatcattt gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt
     5761 gaccagaatc gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc
     5821 gatctctttt tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc
     5881 aaaatatttc attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat
     5941 ttcatgtaaa gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg
     6001 cttttttgca gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac
     6061 gctgattggt aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt
     6121 tccttctttt ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc
     6181 caataactgc gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat
     6241 gatcagttca ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata
     6301 aggagacaaa gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa
     6361 cgatccttct tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc
     6421 agtcaagaga ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt
     6481 cattcttttt cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt
     6541 tcataccctc gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact
     6601 atttttacat ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg
     6661 tggattgcta cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt
     6721 tcagaggaag cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc
     6781 actcgatgac tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt
     6841 ttacttccgg caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg
     6901 attcgttcga cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt
     6961 cctctcttgt gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac
     7021 gtgttacgca aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt
     7081 caattatact tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa
     7141 aatatttctt catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg
     7201 atacttgttt tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa
     7261 cacaatatgt agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata
     7321 gataataaga atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat
     7381 aattgtatgc aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa
     7441 gaagtcaata tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa
     7501 agtgttcctg tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg
     7561 cgtaaacttg ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg
     7621 cttgagacag caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca
     7681 atgcaatgat ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga
     7741 gtcttaaaaa tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag
     7801 tcaatggtca aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa
     7861 atgtcaaacc aaatacgatg acttttctat ctattatgga aaaaatcaat tacttgatcg
     7921 atcaagaata tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc
     7981 tatatcaatt tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat
     8041 tttattctca atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag
     8101 atcgagtagc cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc
     8161 ttgcagacga aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg
     8221 gacgtaaacg tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga
     8281 actctatcgg gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag
     8341 ggattacttt atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg
     8401 caagtggtgt cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat
     8461 taggtcagcg tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg
     8521 ctttcctttc agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc
     8581 ttggcgtgat cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt
     8641 tatttagtcc atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta
     8701 cagctgagta tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc
     8761 gtgatcttga aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca
     8821 atattgatac agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat
     8881 gttcagagat tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag
     8941 tattaggaac ggatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa
     9001 gccctaactt tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg
     9061 catctgatat cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg
     9121 gattaggtgc catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag
     9181 aggattcatt agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg
     9241 aaagcaacaa tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt
     9301 atgctgacgg cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa
     9361 aagtcaaagc attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga
     9421 aacaagcagt aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg
     9481 gctcaatttc atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg
     9541 aagaacgcca agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg
     9601 agacgattcc gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt
     9661 atgcaatggc acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg
     9721 aaatcccaga aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga
     9781 atattttaag acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct
     9841 ttactgatga tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc
     9901 atagatagat tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat
     9961 tatgaagcaa taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag
    10021 ctgacagagc aattttggct agatacacgt atcccacttt caaatgacct agatgattgg
    10081 cgtacattgt ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg
    10141 ctagatactg tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc
    10201 catgaagaag ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat
    10261 tcttctattt ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca
    10321 aatacgaatc cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt
    10381 acaccgctag aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt
    10441 ttttatactc ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc
    10501 aaactaatca tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta
    10561 ggattcaacg agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt
    10621 ctttatgagc tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc
    10681 tggacagaag aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta
    10741 gggatggatc cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg
    10801 atttcaacag gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta
    10861 ggtcatgtag aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt
    10921 ttaattcaaa aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac
    10981 actgaactct tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact
    11041 tttccgaaac atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg
    11101 tcttaatcac tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc
    11161 ctgctccaat caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata
    11221 acaacggctt gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat
    11281 acaaagcttg aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa
    11341 aagggccgac atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat
    11401 aaggctgatc gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta
    11461 agatcgtact gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta
    11521 actctttacg aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat
    11581 ccttgaacat aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca
    11641 taaatgaaat gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat
    11701 tactaagaga taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt
    11761 cccgctgcgc caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct
    11821 gggtcaattg aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa
    11881 ctaaaatgat tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct
    11941 tttcgagaaa aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg
    12001 gtaaaaaaac tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa
    12061 taaagtacac gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt
    12121 gtttaaaacg tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt
    12181 taacaaagag ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat
    12241 attttaatga gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta
    12301 tactgcagtc cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc
    12361 cagccaaacg aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag
    12421 gacatctatt ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt
    12481 gatatttttg tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag
    12541 atcaatcgta tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt
    12601 cacatcttcg attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc
    12661 ttttctatgg ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt
    12721 cactactgtg agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc
    12781 catgccgatg gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc
    12841 cgtcaccgat tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac
    12901 cacttgagca atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc
    12961 taaagcatct cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc
    13021 tcccatacaa actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc
    13081 aaaaccgatt gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat
    13141 tgtcaatacc atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga
    13201 gcagacaaaa aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg
    13261 tcatagtcct ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca
    13321 aacggcaaaa tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc
    13381 tgatttcaca acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat
    13441 cgatgatcac aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta
    13501 caggtccttc aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga
    13561 cgatttcttt acttttcata attgaaccga ttcccattga aacagctttt gttggtacat
    13621 cttctgcttt atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta
    13681 ctgaagtcgt tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc
    13741 cgattcctaa gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc
    13801 atgctgcttc cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt
    13861 tatcaaataa ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc
    13921 caacgtactc atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca
    13981 tttctttata taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt
    14041 tgttcatacc ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta
    14101 cacgaatgat ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt
    14161 ctttttttct ttttacaata attagtatag accaaattgc atttttttca ttttcttgac
    14221 aataaatagg aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca
    14281 tgaaaaccaa gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa
    14341 ggagttatta tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc
    14401 ctctatgaag gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa
    14461 aacggagccg gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac
    14521 ggacagatcc attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc
    14581 gatgaaacat taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag
    14641 gaagaaaaaa ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg
    14701 gaaaaagctg ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc
    14761 ttagttgctg agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg
    14821 aaaaatctaa gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac
    14881 ccagatgttc tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg
    14941 ctgacgcagt ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa
    15001 tttcttgatc agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat
    15061 actggccatc taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc
    15121 caatatcatg ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa
    15181 gccggttccc gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg
    15241 ctgacgcctc cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc
    15301 acgacattag cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca
    15361 cccatcaatt tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt
    15421 ggaaaatcta ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc
    15481 cacttcccag ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca
    15541 ttagaaacac cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta
    15601 cgtcgccaac tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca
    15661 ttaagtggtg gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat
    15721 ttactgattc tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa
    15781 gacgggatac agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat
    15841 caagatcttg tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt
    15901 gaaaaaaagg agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc
    15961 caattagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat
    16021 ttggctattc ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt
    16081 tattttcgag atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt
    16141 gaaaaacaag cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc
    16201 ctagcaatga accagccgca cctccaataa agtacataaa cataaataca gtattatttc
    16261 ggttgcttgc tgcttcacct agcccttgta ctcgtgtttg attggcgact tgaccaaatt
    16321 gattcccgac gtctaataaa ataatagcta aaagcaaaat ggccaaatgg cttccgccaa
    16381 ccaataaagc aataaagctt attgtttgca taacaagtcc catatacacg atttttcttt
    16441 cagaataaga atctgccagt cgtcccacga taggtgcagc aactgctcca gacaaaccaa
    16501 aaatcgctaa tatccctgct tcgaatgttc cccaattgta ggcgggactg ctgatataaa
    16561 aaatcaacgt agaccaaaag atcgaaaatg ttccaaacat gaaaaatcca ctgactgccg
    16621 cttctcttaa tagacgttga gaagcaacta aatgcggcaa acttttaagt gaatcaaggt
    16681 atgaaacagc tgtactactt tgctggacag aacgcggcat tttccagtaa agcagaagtg
    16741 taagaattcc gactgcacat aaagcaaata aatagatcat ccgccaagaa aaaagactgg
    16801 caacaatacc agataccgtc cgagaaagca aaatccccac gagtaaaccg ctaagcaatg
    16861 tacccatgac cttccctctt gcttttggtc ccgctagtac cgcaccgtaa ggaatgatga
    16921 tttgcggcac gatcgaaagt aatcctatta aaaaagaagc gcatgcaaat aacgtaaacg
    16981 aaggcgcaaa aaaagcggcc aacagagaga cggcagataa cgctgccatt cgaatgatca
    17041 attttggacg atccaccacg tcccctaaag gaactaaaaa cagtaaccct aacgcatagc
    17101 ctaattgggt caacatcgtc aatatcccga ctgcacttgt gctaacagat aaactagctg
    17161 ctacttttgt tccaatcggc tggatgtaat acatattggc aacaacaacg ccacaagtga
    17221 ttgataatag taaaataagc gaagttgtta aaggtttttt ttcattactc ttcattgatt
    17281 tcccccattt cctatcttag tttacaactt atggaaaaga ttggaaatag aaaaatacaa
    17341 ctattcgcca cacttattat ttgtgctttt gttttccttg ttttttcttg cgatctttat
    17401 aaatgaagac aactaaccct actagcacag taacacttgc aaccaacgtg actgcagttt
    17461 tgttatatcc gtgaaacaga gcttctccac cacacgcata caacacagac gaagggatga
    17521 ctccaatcac gatcgacaat atgatttgct gtagaggcaa tttcatcgca tctgcagcaa
    17581 aattgaccat agaagatgga ataaccggta tcatatagcc taacattaca cctatttttg
    17641 gatgtttcat ttgtcggatt gcttgcaccc aatgattcgt ttcggttttc ttatctaaaa
    17701 attttaagcg atgcataagg aaaattgcag tgatgttacc aagagcatta cccaaaatgt
    17761 tgatgatcgc acctgtcagt ggtccaaagc tcaaaccagc aatgactcca actactgaag
    17821 taggaatacc tggaacagca cacatcaccg ctgtcaatac gatcaagagt atcgatgcaa
    17881 aagtcccatg cgaacgaata tgtgcgagta gctctgcctt actcgctctc ggatttaaaa
    17941 ataattggat gtctgtctga tattctaaat acaaccgata gcccactacc agtatcagaa
    18001 tgattcccaa cgagatcaat atcaatcgga ttattttctt tttcatattt cctcctgaaa
    18061 aaaatctata ttcattaaat aatagtttaa cataaaaaca aaaaagcacc gaaatccaac
    18121 ataggatctc agtgctttta aatagtaaaa attatttaac agttacagat gcgccaactt
    18181 cttcaagttt agctttcaat tcttcagctt cttctttaga tacgccttct ttaactggtg
    18241 caggagcgcc atcaactaca gctttagctt ctttcaagcc taaaccagtt gcttcacgaa
    18301 ctgctttgat aactttaact ttttggtctc cagctgcagt taattctaca gtgaattctg
    18361 tttgttcttc agcagcagca gcgccaccag cagcagctac agctacagga gcagcagcag
    18421 atacgccaaa ttcttcttca atagctttaa ctaggtcgtt aagttctagg atagttgctt
    18481 cttttaactc agcaacgatg ttttcaatgt tcaatgccat tataatttcc tccgtttaat
    18541 tgttcttttt tatttaatgc tttatgcggc aattaagctg catctccatc ttctttgtct
    18601 gccactgctt tgacagcgta agccacgttg cggactggcg cttgtagtac agatagcaac
    18661 atagatagaa gtccttcgcg atttggcaat tttgccaatg ctgtgatttc ttccaatgaa
    18721 gagactttac cttcgatgat accgcctttg atttctaatg cttttgcttc tttagcaaat
    18781 tcgtccatga ttttcgctgg tgcgactaca tcttcgttac tgaatgcaac agctgtaggt
    18841 ccagtgaata cttcgtccat gccttcaagg ccagcttttt tagctgcacg tgaaagaata
    18901 gaatttttga taactttcat ttctacgtta gcatcacgaa gttgtttacg taaacgtgtt
    18961 acttcatcaa ctgttaatcc gcggtagtca acgactacta ctgatgcagc tgctgtaaat
    19021 ttttcgctta cttcatcaac gattgctgct ttttttgcga taattgcttc actcattttt
    19081 gagtttcacc tcctgaattt tgatggaaga gttttttgca ctgaaaaggc aagtcacttt
    19141 tatagttact ttgctcaacc agtattattc atcacctatt cacttcgttc atagtgattc
    19201 tactcgcttt gctcgaaagg aaactccttt cgctttgctc aaccagtatt gttcatcatc
    19261 tcttcacttc gttcatagtg attcacaata aaaaactcta tgccaccgca gacatagagg
    19321 gactattgaa tatattcaat aaacgtcctc ggtaggaaat taaggcttga tgccacctac
    19381 tgtcttcggt acaagttaat tattaacctc aactagctta gcatgctaaa ctagttgagt
    19441 caagaattat ttttcagttg gatcaatcaa cattctaata tttaattaga aagaagcttg
    19501 gtcaacatgg ataccaggtc caaatgttgt tgtgatcgtt acatttttca tgtattgacc
    19561 tttagctgct gatggtttag cttttagcaa tacatcatgg attgctgcaa agttttcaac
    19621 taatttttca tcgtcaaatg aaacttttcc gattggtaca tggatatttc cagctttgtc
    19681 cacacggtaa gttactttac cagctttcac ttcgtttact gcttttgtta cgtccattgt
    19741 aactgtacca gttttagggt ttggcattaa gccttttggt cctaatactc gtcctagacg
    19801 acctacagta gccatcatat caggtgttgc aacaactaca tcaaagtcaa accatccgtt
    19861 ttggattttt tgaaccatgt cgtcgtcacc tacgaaatct gcaccagctg cttcagcttc
    19921 ttttgctttg tctcctttag caaaaactaa aacagtttgt gttttaccag ttccgtttgg
    19981 taatacaaca gcaccacgga tttgttggtc tgctttttta gggtctacgt tcaaacggta
    20041 tgcaacttca actgttgcat caaattttgc aatgtttgtt tcttttgcta aagctactgc
    20101 ttcggcaaca ggataaactt tacttgaatc aacttttttt aatgcatctt gcattttttt
    20161 gctcttttta gccattttgg ttcctccttg attgtggtta taacggttgt acctcccacg
    20221 tgttctatat ctttcaatat agagccaact gagaagctgc tgtcttaggg tgtatagatt
    20281 attctacagt gatccccatg cttcgtgcag ttccttcaac catgcgcatt gctgcttcaa
    20341 cgttagctgc gtttaggtct tccattttta attcagcgat ttctcttact tgatcgctag
    20401 aaactttcgc aactttattt ttgtttggtt cgcctgaacc tttttcgatt ttagctgctt
    20461 ttttcaatag aacagcagct ggcggtgttt ttgtaacgaa tgtgaatgaa cggtcttcgt
    20521 atacagaaat aacaaccgga atgatcaaac ctgcttgatc agctgtacga gcgttgaatt
    20581 cttttgtgaa tcccatgata ttgatacccg cttgacctag tgcaggacct actggtggag
    20641 ctggagttgc tttacctgca ggaatttgca atttaacgat tttttctact ttctttgcca
    20701 cgagacatac ctccttaagt ccgtgatgtg gttaattgga gatgcatatt tctcctccca
    20761 cgttttatct gatctgaaaa aatccagaca aataatgtgc aaaacaatgc acactggtac
    20821 agtatagcaa atttatttgt tttttcaaga tttttttctg ctatttttac attttgcttg
    20881 aataaacgct aaatttattc taaaaaaaca acaatttttc gttctcattt cttcttttat
    20941 gttacacttg cgctagcatg gttcaacaga tgaaagcaaa caataacacg gttttttaga
    21001 cccttgtaca aagattccta cataacaaaa tgaggtggag aaaatgaaag atttaaggta
    21061 tagaagtgtc tttgatatta ttggacctgt gatgattggt ccaagtagct ctcacactgc
    21121 cggagctgct agaatcggga aaatcatacg tagtatattt ggcgaacagc ctgattctgt
    21181 agatatttat ctatatgaat catttgcaaa aacctatcgg gggcacggaa cagatattgc
    21241 attagttggc ggcctgttag ggatggaacc cgatgatgaa cgtttagctg attctttgaa
    21301 attggcatat gaagccggta tggaagtctt gtttgttcct aagagtgaaa aagccgaaca
    21361 tccaaactct gtaaagatgc ttctaaaaaa aggaacacga aagctttcag tcactggcat
    21421 ttcgatcggc ggtggcaata tccagatttc tgaattgaat ggattcaaaa tttctttaag
    21481 catgggtact cctaccttga tcgttgtcca ccaagatgtt ccaggtatga ttgctaaagt
    21541 gactagtata ctttctgaga ccaatacgaa tatcggcact atgacagtga cacgggaatc
    21601 aaaaggtgaa aatgccatta tgataattga agtagatgat cctcaagtag aagaaacggt
    21661 aaaaagactc aaacagctgc caaatatcga cagcgtaaat tatttcgatt aaggagctaa
    21721 aaaaatgttt ttatcgattg aagaattagt agatcaagca aaagcttatc caagtgtagc
    21781 agaattgatg attgcagttg aaatggaaat gagtggccgt tcccgtagtc acatcatcga
    21841 gatcatggaa aaaaacctct cagtaatgga acaatcaatc aacgagggaa aagaaggtgt
    21901 tacctccgtc acaggaatta ccggtggtga tgcaaaaaaa ttagatgatt atttaaatga
    21961 ggcagacttt ttaagtggag aaacaatctt aaaagctgta cgcaatgctg tagctgttaa
    22021 tgaagtcaat gcaaaaatgg ggttgatttg tgctacaccg actgccggaa gcgcgggtgt
    22081 tgttcctggt gtgctgatgg ctgtccgaga tcggcttgat ttgacacatg aacagcaatt
    22141 ggaattctta tttaccgctg gcgctttcgg gttagtcatc gcaaacaacg cttctatcag
    22201 tggtgcagaa gggggatgtc aagcagaagt tggttctgca agtgcaatgg cagcagcagc
    22261 tttggtttgt gcaaaaaatg ggactcctca tcaagcagca caagcagtag ccattacatt
    22321 gaaaaatatg atgggactga tttgcgatcc cgttgcaggc ttagttgaag tcccgtgtgt
    22381 caaacgtaat gctttaggct cttctcaagc ttttatctct gctgatatgg cattagcggg
    22441 tatcgaaagt gtcattccgc cggatgaagt cgttgctgca atgtaccaag tcggacgtca
    22501 aatgccgtcg attttcaaag aaacagcaga aggtggctta gctgttacgc caactgctaa
    22561 acgacttgcg gaagagattt taaataagag ggcgtgataa agctccttaa tttcgagtat
    22621 taagagatta gttatcttca taacaaaaaa cgaagagagg actaattgcc cttctcttcg
    22681 ttttttatta atccatcaat ttcgtatttt ttacttcatc gatcgttttt gttcctgcaa
    22741 gttgcatcgt gatcgacaat tctttgttca ggtgttcaaa aacagatgtt actccttctg
    22801 ccccacctag attcaaccca taaatcactg gtcggccaat cgcaactaag tcagcaccac
    22861 ttgccagagc tttgaagaca tgctctcctc gacggacacc agagtcgaaa atcactggta
    22921 cacgtttgtt gactacttcg gcaatttttg gcaatacttc aaaggacgca ggacctccat
    22981 ctagttgacg tccaccatga ttggataccc agataccatc tgcccctgca gagatagcga
    23041 ttactgcatc ttctggcgat tgtattcctt ttacgataac tggaagatgg gtgatttctt
    23101 tgattgtttt gatgtcatct ggtgtcaagc cttgttttgc agctgcataa atttctgcga
    23161 ttccttttcc ttcgccatta ccacttgcac tttgttcgct atacgcagct aagttaggca
    23221 ttggtaatgg aaattggaat tgattgatga cgtcttcttc acggtaacca ccgagcgttg
    23281 aatctgccgt taaaatgata gctttagccc ctgcttctac tgctttgttc aaaatgaact
    23341 cattgaatct gtcgtcttta ctcatatata attggaaaaa ctgcggtgcg cctggcgctg
    23401 catcggctgc gtctttgatt gtcgtatttg cataagtgct gatggaaaag attgatccag
    23461 cagcagctac tcctttagct gtatcagctt cccctttttc atgtgccaat ccttgggccg
    23521 ctgatggcgc ttggataatc ggtgtatcta attcaattcc gaaaacagat gtatgcaaat
    23581 cggctgagtc gattccgcgt aatatccgtg gcatgatttt ctttgtatta aaagaagtcg
    23641 tattttcttt catcgtccac tcatcttcag aaccaccgcg aatatagcca aaagctcctg
    23701 cttccatgcg ctcttttaca cgaccttcta gttaggcaat gtttacaata tcgattggat
    23761 gttcttcatt acttgtatga taagtcatag tagatccttc tttcatcttc ttgattacat
    23821 ataaatcata ctaaacttac aaaaagtaag caaacattta ctctcagata cttttatcgc
    23881 tattctttcc aaataaatcg acttacaagc tgatctactt cttggttttc atgaagcata
    23941 ctatgagagg cattttcacc tgtcactact ttttcctcta tttcatttcc gcgttgcttc
    24001 aacaacgcat agaccgctaa tgcactgttt aacggtactg ttccatcact caagtctgtt
    24061 tcatctagtt gaccagcaat caagaaaaat cgagtggtta taggaatatt accgatcatt
    24121 tgctggtaat cttgatagcg gttagaaacg acagcaggcc cctttgctaa ctcatctgtc
    24181 agagactgcg cactatcatc gacaaaatca ttaaaaggcg cgccaattgc tgttaatgtc
    24241 tgaacttgtg gcaatgacgt atcctgtcca tacgttccta aatagcgtag tgcactaact
    24301 cctcccatgg aatgccccac tatgttgact ttatctatgc cgtagttttc tttcagatac
    24361 aaaagacaag ttttgatcca ttctgcttga ttccactcgt tatttttatt atcttcaaac
    24421 aatacttgga ccatcggatt gtctgctttt ccagacaaat ctcctgttgc ttgtatttgt
    24481 ccatctgttc ctactgttaa aaccaattct tttactgctt gctgattttt ttccaaacgc
    24541 cgcaacatcc ctttaaagga attttctgtc ccgctgtagc cgtggaaaaa aagtgttgga
    24601 atagtggttt tttctatcac tttactggat tctgccgttt tttctttatc agaaacttca
    24661 gtagtattta tcttcgtcgt ctggcaacca cttaacgcaa acaaactgat aaggactaaa
    24721 gctgctgcag ttgcacgttt gcataaattt ttgattggtt ctgtcataca cctcttcctt
    24781 tccatataga aaaaagaaag acttgaaagg ccaatcatat gatcgacatt tcaagtctct
    24841 ttattaatcg attttatcta cttgatcgaa atcaagttct gtactggttt cacgtccaaa
    24901 catatcgatg ttgactttca atttttcttt ttctccgtca acttctgtta cttgtccttc
    24961 taatccagca aatgcgcctt cgatgatttt cacgacttcg ccaacttcta cttctagatc
    25021 attttgtcgc aggctcatac cgatagaacg caagatatga ttgacttctt ctggaagtaa
    25081 tggtgctggt ttgcttccgg ctccgtgtga accaacgaat cctgtcacac caggtgtgtt
    25141 gcggacaaca taccatgaag cgtctgtcat gatcatttca acaagtacgt atcctgggaa
    25201 tgttttatga acgatttctt tttctttccc atttttgact tcagtttctt tttcttcagg
    25261 aacaacgaca cggaaaataa agtcggccat tttcatactt tgcgcacgag attcgatatt
    25321 tgcttttact ttgttttcat atcctgaata tgtatgcaag acataccagt ttttttcaaa
    25381 agattccatt gaaggctcct catttcattg taaaataaaa aaacctcagt acacccgaag
    25441 tttttctttc gcttcattat agcactaaaa gtacgttgtg gctagcgagt tattttaaaa
    25501 tccagccaaa taagctttgg atggctgtat ccattacgta aaacatcaat ccgaaaataa
    25561 tagaagtttc gatgactact aacgtatctt tacggagttg ttttctgctt ggccaagtta
    25621 cttgtttgat ttcatctttt acgcttttta gaaatttcat gagacaacct cccttgctac
    25681 tgtttatttc gtttctttat gtaatgtata ttgattacaa tatttacaaa acttattgat
    25741 ttccaaacgt tcgccgcttt ttccttcact gactgatttt gtgtagttgc gagacccaca
    25801 aacggaacaa gcgagtgctg cttttttctt tgccatttac ttctgccccc ttgctgagaa
    25861 tcagacttat acttcataac agtatcacgt aactttttac ttgtcaattt gtgaaataaa
    25921 ctatctattg ttttggataa aattatgata tgattaattt gaaattaagc taaaatgaga
    25981 caattggagg tatagcgatg ctattaaaaa cacttgtata taagaaaaaa gatctgacaa
    26041 ccgttacaga gactagtaca ttagaagaag cattaaaaat tttagaaact tccggatacc
    26101 gttgtgtgcc gatcttagat tcttcaggta atattttccg tggaaacatt tacaaaatgc
    26161 atatctatcg tcataaagca aatggcggtg atatgacgct tcctgtcact catcttttga
    26221 aaaacgctac aaagttcatt tacctggata cttctttctt caaagtattc tttacgatca
    26281 aagagcttcc ttatattgct gtccttgaca gtgaaaatca tttttatggt attttgacac
    26341 atagtacgtt gcttaatatg ctctctcaat cttggagcgt agaacaagga agctatgtac
    26401 tgacaattgc ttccacagga aagcaaggag atttggctgc tattgcaaaa atcatttcaa
    26461 aacacagcag cattgccagc tgtattactc ttgatatcga aaaagacgaa tacattcgac
    26521 gtacattgat cacgctaccg gctaacactg aagcgaaagt atgtgaaacg atcgtcaacc
    26581 acttagagaa aaagaattat aaagtggtag aaattgaaaa tttgcaaaac caagaagcat
    26641 aatcaaaaag cgaaaaaaga aaacagctaa tctgctttct tttttcgctt tttataatta
    26701 gacacgcttt tcaaaatcaa gcgcgcgatc taaataatac atcaatggtg ctgaaacagc
    26761 catgacgata aactcactta atgcaagcgt gccataggtt gtccaaaatg cttggccacc
    26821 tgttggaact aacatataag caatcagtcc catgctgatg gtaaagaaga tcgtgttcaa
    26881 tactaaacgc atctttacat ttttcacttt gttttgcaat aaagctgtca ggcctaaaga
    26941 aagcaatgtt tggccgccac caaaaaccac atccatgata ccaaaaccaa agaaggcatt
    27001 atagacgatg accccgccta aaactcccca aagcaattta cggttaaaga caactaaatg
    27061 attcaaactt tctgaaatac gaaactggat cggccctgag gatacaggcg caactaaaat
    27121 agttaacgct agataaagag ccatgatgat gccatttaca acgattacgg ataaccgttg
    27181 tctgcttttc gttttttgca ttttctctcc tcttttctcc tagttttttt aacgcgggat
    27241 ggttgatgaa ccgcggttat gtgaaacaaa caataattat aacagacttt tcatttattt
    27301 caatccgtct tatctgcttt taaaaaagca aaagcttaaa caatgtaaac gtttgctaaa
    27361 aatcgtggga tatggtataa agtaagtgta aggggtcata agaattccca gtaaagcaga
    27421 cgaagtagga tctgcaagaa cagaattgtt gtgaaactta aataaataaa aaaggatgtg
    27481 ggaatttgag caacctctta ctagagaacg gtctagcaag cagctgaaga tctaagtgga
    27541 catgccgaac cttggaactt ctccttacgt aagtaatgga tcatgcgctt gctaggcttt
    27601 tttgtgtttt tcggtcttcg gacctttttc attttcctag acttctgcta taataaccaa
    27661 gaattaattt tgtaacagat ttttttgaaa aaacaagttt ctttcattca aaaaatagaa
    27721 aaagcttgct aattattggt taaccagcaa acaggagtat taaaatatct tgttttattg
    27781 aatagcagga ggaaatcatg aaattccatt atcatatatt gatcaatcca tcggcaggca
    27841 gcggaaatgg ctataaagta gcagaaagaa ttttaccggt attaaaaaat aagcatattg
    27901 actatacact tcattatagt gaatacaaag gacacgatgc tgagattgct gaaactttgg
    27961 caaaagaaac attgattcct tggatagaag aagaacagaa aacaatcgat actttccccc
    28021 ttttgatcat cgtaggaggt gacggcacct tacatcaggt acttgatact ttctatcaga
    28081 tggaagtaga attcccagtt gcttatattc cagcaggttc tggagatgat tttgctcgag
    28141 gggcaggcct tccaaaaaat cccaaaaaag gacttcagct gattttagca gctcaatcac
    28201 cagaaaaagt ccacataatg gcttatgaag aaaaaataag tgaaaaaaaa ggaatagctg
    28261 ttaataattt cggtatagga ttagatgcag ctatcgtcca tgcgacaaat cattcttcca
    28321 caaaaaagcg attgaacaaa tacaacttag gatcattttc gtatttattc tctattctcc
    28381 gtgctttatt cactcaaaaa ggatttccaa ttctggtaga tgcaggagga aaacaattaa
    28441 gtttttcaaa cgcattttta tgtacagcaa cgaagcatcc tttttttggc ggaggaatcg
    28501 cgattgttcc aactgctgat gcgtctaaac cagtcattga ttttgtcgtg gtcgaacgaa
    28561 tcaatttctt taaaatcttg tggctcattc ttttacttat acagaaaaaa caattgaaat
    28621 cgaaatattt ccatcactac acaacaaaca gattacgtat cgtttcaaca acaccgcaac
    28681 atggtcaaga agatggagaa gaaatggaaa aacgaccttt tgatatcaca ataaccacaa
    28741 ctaaacagtt gttttggtgt taataaaaga ggttttgtca gaagctacat ctggaacacc
    28801 gtttattcgg aaaaaaggac tgtgacacga cttttgtcac agtccttttt ttactatgca
    28861 gttaattctt tgcccaattg ggttgccatc gatagtttct ttctttaccc aaaataaatt
    28921 ggatcattga aaacaccgca gcaaaagcaa ctaagacaca accattgaac caaaaagatt
    28981 ctgcaaatgt ttgattgtaa ctgagtgttt cactgatcaa gttattattt tgaacaaacc
    29041 aatatgttgg tgtgaatgcc gctattttgc taaccatttc tggcaagtat tctgctggta
    29101 caaacacccc accgataaag caactgcctg tgataaaaat atctttgatc ccaccgctgg
    29161 tttctgagtt ttttaccaat gaagtgatac aagccgaaaa gctaatgatc ggcaataaaa
    29221 acaagaaagt atttaaaata tgataccata ccgttatatc ccacgaggtg tggatcacta
    29281 acagaaccat tagaagtaag aagccactga acaacagcag cgagtaacta atcaaactaa
    29341 aggttacttt gcgattcaag tttcgtttgg taatgggcgc actctggttt cgtttactga
    29401 tctctttgcg attaaatgct agattgatga cgctgaaacc gctaaaaatg gatagaaaca
    29461 acccataaga caagaaatta aaggttgtgc ccgtcaacag ctttttcatc cgctgactat
    29521 atcccgcact tagctcaact tctccagtaa ccgagagtgc catttgtgtc tgttccatag
    29581 ctgcatcgat tgttttatct tcttcttgaa acagaatcag tgtttgcaaa aaggtgttca
    29641 cttgctgtgt aacaactgtt ttagaaaagg catctgggcg actctgtatc ttgatcgtcg
    29701 gcatctttcc tacttcgact tgttcggtaa agtctgcagg cagatgtaag ataatgttta
    29761 cacgattaaa ataaagagca tcatcgattt ctttttgtga ggttccatcc aatgtaacga
    29821 ctgtttcctt gtcattcaaa tatttacgaa ggccatcgac tacaggcgac tcttcttcac
    29881 taatcaaggc gattttcgca tcttgcagag aggtattttc aacatcaata ttttgcgcaa
    29941 aaaagaatgt ggcaacaaca gtgataatta cacctatcag taaattcgat ttatttgctt
    30001 taagcaatgt ccaaatcgtt ttataaagcg tcataacgca cccctctttc catccaagca
    30061 tttcctaacg caaaaaggaa aataaatccg atcaaccata gaagattggt ataaaaacta
    30121 gccaaatcag tgtagaaata aagttt
//
LOCUS       DAPCMU010000020        27655 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPCMU010000020 DAPCMU010000000
VERSION     DAPCMU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.020"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(217..1161)
                     /locus_tag="RD744_000746"
     CDS             complement(217..1161)
                     /locus_tag="RD744_000746"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDU0556775.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1457..2401)
                     /locus_tag="RD744_000747"
     CDS             complement(1457..2401)
                     /locus_tag="RD744_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556776.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2622..3821
                     /locus_tag="RD744_000748"
     CDS             2622..3821
                     /locus_tag="RD744_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0556777.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPTNTSILLVGANL
                     GTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEI
                     KEQLTTK"
     gene            3843..5858
                     /locus_tag="RD744_000749"
     CDS             3843..5858
                     /locus_tag="RD744_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HDU0556778.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5941..7314)
                     /locus_tag="RD744_000750"
     CDS             complement(5941..7314)
                     /locus_tag="RD744_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDU0556779.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7340..7624)
                     /locus_tag="RD744_000751"
     CDS             complement(7340..7624)
                     /locus_tag="RD744_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0556780.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7626..9641)
                     /locus_tag="RD744_000752"
     CDS             complement(7626..9641)
                     /locus_tag="RD744_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0556781.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9820..10125)
                     /locus_tag="RD744_000753"
     CDS             complement(9820..10125)
                     /locus_tag="RD744_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDU0556782.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10214..11245)
                     /locus_tag="RD744_000754"
     CDS             complement(10214..11245)
                     /locus_tag="RD744_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0556783.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11452..12327
                     /locus_tag="RD744_000755"
     CDS             11452..12327
                     /locus_tag="RD744_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0556784.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12479..13321)
                     /locus_tag="RD744_000756"
     CDS             complement(12479..13321)
                     /locus_tag="RD744_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0556785.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13413..15290)
                     /locus_tag="RD744_000757"
     CDS             complement(13413..15290)
                     /locus_tag="RD744_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0556786.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15287..16708)
                     /locus_tag="RD744_000758"
     CDS             complement(15287..16708)
                     /locus_tag="RD744_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0556787.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16711..20100)
                     /locus_tag="RD744_000759"
     CDS             complement(16711..20100)
                     /locus_tag="RD744_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDU0556788.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20357..21748
                     /locus_tag="RD744_000760"
     CDS             20357..21748
                     /locus_tag="RD744_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556789.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21821..22468)
                     /locus_tag="RD744_000761"
     CDS             complement(21821..22468)
                     /locus_tag="RD744_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDU0556790.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22476..23162)
                     /locus_tag="RD744_000762"
     CDS             complement(22476..23162)
                     /locus_tag="RD744_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556791.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23168..24241)
                     /locus_tag="RD744_000763"
     CDS             complement(23168..24241)
                     /locus_tag="RD744_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556792.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24463..25137
                     /locus_tag="RD744_000764"
     CDS             24463..25137
                     /locus_tag="RD744_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0556793.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25142..26482
                     /locus_tag="RD744_000765"
     CDS             25142..26482
                     /locus_tag="RD744_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0556794.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26631..27617)
                     /locus_tag="RD744_000766"
     CDS             complement(26631..27617)
                     /locus_tag="RD744_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDU0556795.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8061 a         5236 c         5015 g         9343 t
ORIGIN      
        1 ttttttaatt aaccacagat aaacattgat aacgattcgc ttattgtaat caacagatta
       61 aatttcatat gttcatatgt acggatacat tttcggaccg attaatttta accattctgt
      121 ttatttgctt acctattaga tatcaataaa aaactgctta acagctattt cagctgttaa
      181 gcagttttag gatttttagt tcatattcat tgtttcttat aatacagtgt tcaatacttc
      241 tcttaaagca cttgttgatt tgctgaacat tgctttttct tcttcgttga ttgataattc
      301 aacaacttca cgaacaccat tttcgtcaac gattgatggc acacctgtaa agatatcttt
      361 ttcgtcgtat tcaccagtta aataagcaga tacaggcaat acagcttgtt cgttattcaa
      421 gatagctttt acgatacgtg cagtactcat accaatacca tagtaagttg cttttttacg
      481 atcaatgatc tcataagcag cattacggac tttatcagca atcacatcta attcatcttg
      541 ggcaatacga tggtcttttt ctacaatttc aaacactggt ttaccaccga cagttgtatg
      601 tgaccatgca gcaacttctg aatcaccatg ttcacctaag atatagccgt ggacgctgcg
      661 tgggtcaact tttaatttca atgcgatttc tttacggaaa cgagttgtat ccaatgttgt
      721 tccagtaccg ataacacgtg atgttggcag accagattcg ttccaagcga tatatgtcaa
      781 aatatctact gggtttgaag caacaacgat aataccgtcg aatcctgatc ccatgatttc
      841 ttttacgatt tggcgcatga ttgacgcatt tgttttaact aaatctaaac gagattgacc
      901 aggtttttgg ttgataccag ctgtgataac aacgatatca gcatctttac attcttcata
      961 gccacctgac catacagcca cattttcatc gccccaagcc ataccatcta aaaggtccaa
     1021 tgcttcccct tctgcttttt cttgattcac atcgatcaat actaattcat tagagattcc
     1081 ttggttgatc attgcataag cgatacttgt tccaacaaaa cctgtaccga cgattactac
     1141 tttacgactt gtttttttca tgaataaaca gctccttttc ataactattt gttactttca
     1201 tgaaaagtat agtcttgttt attcatttta tcaacatgtt aattataata gttgttatta
     1261 taatttttga tggaaacgaa ttcctgaata cgcataacag ataaatgaca aatgtttaca
     1321 cctatatccc ctgataatac aaccaaaagt ttagaactga aaataaatcc aaaaaaattc
     1381 gctgcaacga tataataaga cagcaatcgc acctaaaaca attttccttc atatgcttta
     1441 gtgaaagatt ttcttcttaa tgtttatttc tccattcatt gggagtacat gttgaatact
     1501 tcttgaataa tttgaataaa gaagtcgttt gtttatatcc gatacgatta gcgatttctt
     1561 ccaacgaaaa actcgtttct tgaatcagtt ccaacgcaaa gtcgaatttt ttctttagaa
     1621 ccagttcttg aaaggaagca tcaaaataca ttttcaattt attactaaga tagaccggat
     1681 tgtagccaaa atgagctgct gtttctttta acgtacaaga agtaaaatgc ttatcgatat
     1741 aatctaatac tctaagcatc tcattttctg cgcatccatc tgctgatgaa aagctttgtt
     1801 gttctaataa atgagtcaat tcgatcagta atagtgatgt atagagattt aacgaacgga
     1861 tcttatgact gtatgtatca aaataaatca acagcatatt ttcaatcaaa tgcgtcaaac
     1921 gacttgtttt tccgccgcga aaatgaatga attgtgtatg ttcccctttt tcttttgatg
     1981 catttatcaa aaaagaagaa agtagattat ttgtgcttgc caaatgattc aatatctctg
     2041 tagataaggt ttcttctttt aataagatgt tgattaaaat atcctcttgc cctgtgtatt
     2101 cgattctttg agtagcttct ttatcaatga tcaccacgtc actttcttcc atctctatcc
     2161 tttctccatt aataatttgt aaacatgtac cgctatacat ataattgatt tcaacaaaat
     2221 catgtgtatg ctcgggaact tccgcatatc gacggtgttt actgatatga atatttccct
     2281 gttgaaatag atcccctcgg tggatttttg gcggtgtctg aagatggtaa gctaagttta
     2341 aatcattgat attcattcct tgttcacgct gcagagtttc gattgattct tcgttacgca
     2401 aaaaaaatgt ctaattcagc tagttccaat tgactccctc ctttttgtaa gcggtttctt
     2461 tcgcttatta gttcacgctg tactgctatt atatgcaaag ctatttttgg tttcataaag
     2521 actttctatg gttgggacat gaacttcctg tgattccgct attgagagaa taaccacttc
     2581 atactatatt tttgataagc gcttacaaaa atgaaaggaa catgcttatg aataaaaaga
     2641 tgatgaaatt ttctctgctg tcagtctctc ttattttgac ttctgctggt gcagtcacgt
     2701 ctgcaattcc cagtatgtca gctacttatg ccacgcgttc tttatctgct gttgaattat
     2761 taacgactgc tccctctttt atggtcacta tttttgtttt gattagtagt tttattgcta
     2821 ggaaaatcgg acaaaaacaa actgtgatca tcggattagt gattgcatcg atttatggta
     2881 tcctccccct cttctcacaa agttacagta ttgtcttagc ttcaagagct ggactaggta
     2941 ttggctttgg attgatcaat tctttggcag tcagcatgat tgctgccttt tatcacggag
     3001 atgaacgagc ctctatgatt ggttttcaaa gtgcatttca aggatttggt gcagctttaa
     3061 tgacatttgt tgcaggacag ctagtacagt tcggttggca atataccttt ttagtctacg
     3121 ccatcagctt acctattctt gtcttgtttg ttttgtttgt tcctacacct gaaacaactg
     3181 aacttcaatc acatggcaca gccccaacaa gacaagtaat caaaacgaat cctaaaatga
     3241 ttagttatgg tatttttctc ttgctggtca tcgtggtata taatgctgta tcgattaaat
     3301 tagccacagt cctgacgact tattctctag gaaacgaaac taatgcagcg aatatcttaa
     3361 caatcatgca attggcaagt atggctgccg gagtttgttt tggctggctt cagacaaaag
     3421 cacatcgcta tatgcttaca ctttctttac tactgatggg aatcggcttt gtattgattg
     3481 ccactgtgat aaacctgtat gtgattgggt ttggtgcgat cctaacagga atttcttttt
     3541 cgttattcat tccttactta ttcaaccaag taagtatcca agcgccaacc aatacttcaa
     3601 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
     3661 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
     3721 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
     3781 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
     3841 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
     3901 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
     3961 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
     4021 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
     4081 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
     4141 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
     4201 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
     4261 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
     4321 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
     4381 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
     4441 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
     4501 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
     4561 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
     4621 cttctttact gaatcagatc tggtcagtat cagaagaaac cttcaagtta tgtagtgatt
     4681 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
     4741 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
     4801 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
     4861 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
     4921 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
     4981 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
     5041 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
     5101 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
     5161 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
     5221 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
     5281 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
     5341 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
     5401 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
     5461 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
     5521 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
     5581 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
     5641 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
     5701 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
     5761 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
     5821 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
     5881 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
     5941 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
     6001 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
     6061 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
     6121 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
     6181 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
     6241 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
     6301 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
     6361 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
     6421 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
     6481 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
     6541 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
     6601 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
     6661 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
     6721 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
     6781 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
     6841 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
     6901 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
     6961 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
     7021 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
     7081 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
     7141 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
     7201 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
     7261 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
     7321 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
     7381 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
     7441 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
     7501 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
     7561 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
     7621 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
     7681 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
     7741 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
     7801 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
     7861 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
     7921 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
     7981 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
     8041 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
     8101 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
     8161 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
     8221 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
     8281 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
     8341 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
     8401 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
     8461 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
     8521 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
     8581 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
     8641 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
     8701 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
     8761 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
     8821 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
     8881 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
     8941 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
     9001 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
     9061 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
     9121 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
     9181 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
     9241 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
     9301 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
     9361 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
     9421 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
     9481 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
     9541 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
     9601 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
     9661 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
     9721 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
     9781 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
     9841 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
     9901 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
     9961 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    10021 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    10081 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    10141 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    10201 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    10261 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    10321 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    10381 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    10441 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    10501 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    10561 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    10621 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    10681 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    10741 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    10801 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    10861 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    10921 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    10981 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    11041 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    11101 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    11161 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    11221 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    11281 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    11341 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    11401 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    11461 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    11521 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    11581 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    11641 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    11701 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    11761 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    11821 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    11881 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    11941 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    12001 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    12061 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    12121 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    12181 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    12241 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    12301 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    12361 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    12421 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    12481 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    12541 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    12601 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    12661 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    12721 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    12781 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    12841 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    12901 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    12961 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    13021 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    13081 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    13141 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    13201 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    13261 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    13321 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    13381 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    13441 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    13501 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    13561 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    13621 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    13681 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    13741 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    13801 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    13861 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    13921 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    13981 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    14041 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    14101 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    14161 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    14221 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    14281 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    14341 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    14401 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    14461 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    14521 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    14581 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    14641 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    14701 tttgtaactt tcaacgttcc atcattacag actgtatttt tagggtagag atggatagta
    14761 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    14821 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    14881 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    14941 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    15001 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    15061 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    15121 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    15181 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    15241 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    15301 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    15361 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    15421 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    15481 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    15541 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    15601 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    15661 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    15721 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    15781 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    15841 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    15901 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    15961 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    16021 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    16081 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    16141 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    16201 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    16261 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    16321 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    16381 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    16441 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    16501 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    16561 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    16621 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    16681 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    16741 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    16801 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    16861 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    16921 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    16981 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    17041 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    17101 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    17161 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    17221 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    17281 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    17341 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    17401 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    17461 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    17521 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    17581 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    17641 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    17701 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    17761 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    17821 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    17881 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    17941 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    18001 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    18061 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    18121 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    18181 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    18241 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    18301 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    18361 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    18421 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    18481 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    18541 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    18601 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    18661 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    18721 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    18781 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    18841 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    18901 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    18961 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    19021 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    19081 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    19141 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    19201 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    19261 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    19321 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    19381 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    19441 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    19501 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    19561 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    19621 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    19681 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    19741 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    19801 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    19861 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    19921 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    19981 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    20041 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    20101 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    20161 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    20221 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    20281 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    20341 atgcaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    20401 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    20461 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    20521 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    20581 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    20641 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    20701 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    20761 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    20821 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    20881 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    20941 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    21001 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    21061 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    21121 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    21181 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    21241 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    21301 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    21361 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    21421 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    21481 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    21541 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    21601 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    21661 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    21721 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    21781 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    21841 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    21901 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    21961 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    22021 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    22081 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    22141 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    22201 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    22261 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    22321 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    22381 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    22441 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    22501 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    22561 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    22621 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    22681 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    22741 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    22801 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    22861 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    22921 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    22981 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    23041 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    23101 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    23161 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    23221 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    23281 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    23341 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    23401 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    23461 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    23521 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    23581 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    23641 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    23701 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    23761 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    23821 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    23881 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    23941 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    24001 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    24061 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    24121 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    24181 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    24241 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    24301 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    24361 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    24421 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    24481 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    24541 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    24601 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    24661 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    24721 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    24781 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    24841 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    24901 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    24961 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    25021 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    25081 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    25141 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    25201 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    25261 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    25321 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    25381 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    25441 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    25501 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    25561 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    25621 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    25681 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    25741 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    25801 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    25861 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    25921 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    25981 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    26041 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    26101 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    26161 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    26221 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    26281 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    26341 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    26401 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    26461 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    26521 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    26581 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    26641 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    26701 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    26761 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    26821 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    26881 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    26941 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    27001 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    27061 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    27121 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    27181 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    27241 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    27301 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    27361 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    27421 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    27481 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    27541 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    27601 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtata
//
LOCUS       DAPCMU010000021        26692 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPCMU010000021 DAPCMU010000000
VERSION     DAPCMU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.021"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(19..729)
                     /locus_tag="RD744_000767"
     CDS             complement(19..729)
                     /locus_tag="RD744_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDU0556796.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="RD744_000768"
     CDS             complement(726..2198)
                     /locus_tag="RD744_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDU0556797.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="RD744_000769"
     CDS             2336..2809
                     /locus_tag="RD744_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0556798.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="RD744_000770"
     CDS             complement(2962..3513)
                     /locus_tag="RD744_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0556799.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="RD744_000771"
     CDS             3618..4148
                     /locus_tag="RD744_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDU0556800.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="RD744_000772"
     CDS             complement(4631..5074)
                     /locus_tag="RD744_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0556801.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="RD744_000773"
     CDS             complement(5090..6250)
                     /locus_tag="RD744_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0556802.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="RD744_000774"
     CDS             6757..8457
                     /locus_tag="RD744_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556803.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="RD744_000775"
     CDS             complement(8559..9392)
                     /locus_tag="RD744_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDU0556804.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="RD744_000776"
     CDS             complement(9696..11861)
                     /locus_tag="RD744_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0556805.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="RD744_000777"
     CDS             12121..14415
                     /locus_tag="RD744_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDU0556806.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="RD744_000778"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="RD744_000778"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDU0556807.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            15066..16073
                     /locus_tag="RD744_000779"
     CDS             15066..16073
                     /locus_tag="RD744_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDU0556808.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="RD744_000780"
     CDS             16095..17114
                     /locus_tag="RD744_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0556809.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="RD744_000781"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="RD744_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDU0556810.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18872..20293)
                     /locus_tag="RD744_000782"
     CDS             complement(18872..20293)
                     /locus_tag="RD744_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0556811.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="RD744_000783"
     CDS             complement(20536..22413)
                     /gene="tdc"
                     /locus_tag="RD744_000783"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDU0556812.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22704..23960)
                     /locus_tag="RD744_000784"
     CDS             complement(22704..23960)
                     /locus_tag="RD744_000784"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDU0556813.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24072..24277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24540..25841
                     /locus_tag="RD744_000785"
     CDS             24540..25841
                     /locus_tag="RD744_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDU0556814.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25910..26509)
                     /locus_tag="RD744_000786"
     CDS             complement(25910..26509)
                     /locus_tag="RD744_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0556815.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8117 a         5486 c         4771 g         8318 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca cgccgcaagc ttggatcaag
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggttggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttgttttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaacagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcaga aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgagat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttacctgac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttttcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtgtca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagatc
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtac ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag acgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt tttatttttc ttctaaactt ttttctacct
    18901 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
    18961 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
    19021 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
    19081 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
    19141 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
    19201 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
    19261 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
    19321 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
    19381 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
    19441 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
    19501 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
    19561 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
    19621 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
    19681 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
    19741 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
    19801 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
    19861 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
    19921 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
    19981 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
    20041 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
    20101 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
    20161 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
    20221 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
    20281 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
    20341 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagtcta
    20401 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
    20461 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
    20521 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
    20581 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
    20641 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
    20701 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
    20761 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
    20821 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
    20881 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
    20941 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
    21001 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
    21061 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
    21121 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
    21181 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
    21241 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg
    21301 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
    21361 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
    21421 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
    21481 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
    21541 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
    21601 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
    21661 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
    21721 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
    21781 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
    21841 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
    21901 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
    21961 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
    22021 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
    22081 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
    22141 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
    22201 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
    22261 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
    22321 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
    22381 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
    22441 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
    22501 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
    22561 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
    22621 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
    22681 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
    22741 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
    22801 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
    22861 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
    22921 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
    22981 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
    23041 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
    23101 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
    23161 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
    23221 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
    23281 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctac ttctggtcct
    23341 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
    23401 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcagaatt
    23461 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
    23521 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
    23581 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
    23641 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
    23701 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
    23761 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
    23821 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
    23881 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
    23941 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
    24001 ctgctagcat gtgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
    24061 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
    24121 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
    24181 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
    24241 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
    24301 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
    24361 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
    24421 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
    24481 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
    24541 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
    24601 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
    24661 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
    24721 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
    24781 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
    24841 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
    24901 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
    24961 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
    25021 atttaaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
    25081 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
    25141 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
    25201 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
    25261 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
    25321 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
    25381 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
    25441 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
    25501 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
    25561 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
    25621 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
    25681 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
    25741 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
    25801 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
    25861 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
    25921 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
    25981 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
    26041 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
    26101 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
    26161 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
    26221 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
    26281 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
    26341 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
    26401 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
    26461 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
    26521 cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg tttattataa
    26581 ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat tgacagaaat
    26641 ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa gg
//
LOCUS       DAPCMU010000022        26328 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPCMU010000022 DAPCMU010000000
VERSION     DAPCMU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.022"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(121..996)
                     /locus_tag="RD744_000787"
     CDS             complement(121..996)
                     /locus_tag="RD744_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0556816.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(1105..2874)
                     /gene="aspS"
                     /locus_tag="RD744_000788"
     CDS             complement(1105..2874)
                     /gene="aspS"
                     /locus_tag="RD744_000788"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDU0556817.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(2889..4181)
                     /locus_tag="RD744_000789"
     CDS             complement(2889..4181)
                     /locus_tag="RD744_000789"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDU0556818.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4575..5522
                     /locus_tag="RD744_000790"
     CDS             4575..5522
                     /locus_tag="RD744_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0556819.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(5611..6870)
                     /locus_tag="RD744_000791"
     CDS             complement(5611..6870)
                     /locus_tag="RD744_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDU0556820.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(7041..7487)
                     /locus_tag="RD744_000792"
     CDS             complement(7041..7487)
                     /locus_tag="RD744_000792"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDU0556821.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(7498..9711)
                     /locus_tag="RD744_000793"
     CDS             complement(7498..9711)
                     /locus_tag="RD744_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDU0556822.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(9944..10708)
                     /locus_tag="RD744_000794"
     CDS             complement(9944..10708)
                     /locus_tag="RD744_000794"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDU0556823.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(10709..11656)
                     /gene="prmA"
                     /locus_tag="RD744_000795"
     CDS             complement(10709..11656)
                     /gene="prmA"
                     /locus_tag="RD744_000795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDU0556824.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(11669..12157)
                     /locus_tag="RD744_000796"
     CDS             complement(11669..12157)
                     /locus_tag="RD744_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDU0556825.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(12123..12794)
                     /locus_tag="RD744_000797"
     CDS             complement(12123..12794)
                     /locus_tag="RD744_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDU0556826.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            12932..14218
                     /locus_tag="RD744_000798"
     CDS             12932..14218
                     /locus_tag="RD744_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDU0556827.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            14290..14484
                     /gene="ssrS"
                     /locus_tag="RD744_000799"
     ncRNA           14290..14484
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RD744_000799"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            14562..14846
                     /locus_tag="RD744_000800"
     CDS             14562..14846
                     /locus_tag="RD744_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556828.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            14919..15398
                     /locus_tag="RD744_000801"
     CDS             14919..15398
                     /locus_tag="RD744_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0556829.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            15620..15763
                     /locus_tag="RD744_000802"
     CDS             15620..15763
                     /locus_tag="RD744_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDU0556830.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            16341..17924
                     /locus_tag="RD744_000803"
     CDS             16341..17924
                     /locus_tag="RD744_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0556831.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(18361..19545)
                     /locus_tag="RD744_000804"
     CDS             complement(18361..19545)
                     /locus_tag="RD744_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDU0556832.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19570..20577)
                     /locus_tag="RD744_000805"
     CDS             complement(19570..20577)
                     /locus_tag="RD744_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0556833.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(20749..21108)
                     /locus_tag="RD744_000806"
     CDS             complement(20749..21108)
                     /locus_tag="RD744_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556834.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(21080..21451)
                     /locus_tag="RD744_000807"
     CDS             complement(21080..21451)
                     /locus_tag="RD744_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDU0556835.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(21468..21770)
                     /locus_tag="RD744_000808"
     CDS             complement(21468..21770)
                     /locus_tag="RD744_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556836.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(21787..22257)
                     /locus_tag="RD744_000809"
     CDS             complement(21787..22257)
                     /locus_tag="RD744_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDU0556837.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(22232..22510)
                     /locus_tag="RD744_000810"
     CDS             complement(22232..22510)
                     /locus_tag="RD744_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDU0556838.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(22507..23556)
                     /locus_tag="RD744_000811"
     CDS             complement(22507..23556)
                     /locus_tag="RD744_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDU0556839.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(23513..24442)
                     /gene="tadA"
                     /locus_tag="RD744_000812"
     CDS             complement(23513..24442)
                     /gene="tadA"
                     /locus_tag="RD744_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDU0556840.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            24574..26328
                     /gene="ade"
                     /locus_tag="RD744_000813"
     CDS             24574..26328
                     /gene="ade"
                     /locus_tag="RD744_000813"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDU0556841.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
BASE COUNT         7669 a         5574 c         4768 g         8317 t
ORIGIN      
        1 taagaaaatt tcaaataaaa aagcagtttt tctttgctga ttctactatg gtagaaaaaa
       61 actgcagaca aacgaacgtc tgtctacagc agtatttcat gaatttttgt caaactcgat
      121 ctaaatgcca tcctcataga aacgaccaag tatctggacg ttttctgcaa tacttacaaa
      181 agcttgtgga tcagaagctt tcattgcttc tctaagtaga ggcagttcgt atcgtgtaac
      241 gatcgtcatc aatactgtct gcttatcatg cttataagca ccttctgctt cattgatgat
      301 cgtgatccct ctacgcatac agttttggat ttcatttact actttttctg gatgtttcgt
      361 tacgatcatt acctgcatct tcttttgttt agtataaacg acatcagtta cttttccact
      421 gacgaaaata gacaacgtac tgtaaaacat atattgccag ccaaatagaa ttccggcaac
      481 aaaaacgatc aatgcattga agtagatcga aatagaacca atcgactttc ctgttttttt
      541 acgaatcgtg atactcacga tatctagacc gcccgatgat aggccgtttc tcaacgctag
      601 cccaatacct agtcccatca cagccccccc aaacaacgca cagatgattg gatcggtaga
      661 taggactgtt tccggtaaaa tatggataca aagggaggtc aaagtgactg tgataaaagt
      721 gaaaattgta aacttcttcc cgatttttaa ccacgccaat acaaacaaag gtatattcag
      781 taaatacaaa gttgttgata ccggtacttc gaatcctatc agtgattttg acaaagtcgt
      841 caaaatctga gctagaccag taataccact tgaataaata tgtcctggct gataaaagaa
      901 attcaatgcg actgatgcga tcaacgcata aacgatagat accgaaagct tggtcgtgta
      961 atcatgataa gcccaatttt cgaatgtttt tttcatggaa aagccccgct ttatatgtta
     1021 tggcttcatt ataaagtgtt tttcaattaa atcaaggttg tcaaaagaag cgtattcgac
     1081 aaatcctttt taccttttta tcggctattc ttctatattc gttacatcaa tattcaattc
     1141 gaacaattgt aaaggcgaaa ccatacttgg tgcatcactc atcacatctg ctgcttttcc
     1201 gtttttagga aaggcaatga cttcacgaat attatcttct ccggctaata acattgcaaa
     1261 acggtccaat cctaatgcaa taccgccatg tggagggaat ccgtaatcta aagctgttaa
     1321 caagaaacca aattgttctt ccatttgttc ttttgtaaag cctagagttt ctagtacttt
     1381 ttcttgaagc tcacgagtat gaatacggat agaacctccg cctaattcat aaccgttcaa
     1441 gacgatatca taagcttctg catagacttt tgcagggtct tcagccaata acgggatatc
     1501 ttcttctttt ggcatcgtga atggatggtg agcagagaca tatcgacctt cttcttctga
     1561 aaattcaaat tgtggccaat cagttaccca taagaaattg aatttcgatt catcaattag
     1621 tcctaattct tttcctaaac gtgtccggat agcacctaat gcagcagcga cgatttcact
     1681 tttatctgcg ccaaacatca gcaagtcacc tggttttgca tctgttgctt taatgattgc
     1741 ttctgttgct tctcccatga atttagcaat tggacctttc aatccgtctt cttctacttt
     1801 cagccatgca agccccttag ctccgaattg tcctacatat tgacctaact gatccatgtc
     1861 ttttcgtgag tagcgatctg cagcgccttt agcattcaat gctttcacga caccgccgtt
     1921 ttcaagagcc atttggaaga ctttgaattc tacttctttg actgtatcgc ttagatcgat
     1981 caattccata tcaaaacgtg tatccggttt gtcgctgccg tagcgagcca ttgcttcatc
     2041 ataggtcata cgaggaaatg gcaatgtgac ttcgataccg cgaacttctt tcattacctt
     2101 cgcaatcaat ccctctgtgt aagtttggat ttcttctgct gttaagaacg ttgtttcaat
     2161 atctacttgc gtaaattcag gctgacggtc tccacgtaaa tcttcatcgc ggaaacaacg
     2221 aacgatttga taataacggt cgaatccagc attcatcagc aattgtttaa acaattgagg
     2281 agattgcggc aacgcataga aatgtccagc atggacacgt gaaggaacta ggtagtctct
     2341 tgcgccttct ggtgtagatt tccctaggta aggtgtttcg atatcgagaa agtcattatc
     2401 atctaaataa tgacgaatcg cttttgttgt cgcatttctt aatttgatgt ttttagtcat
     2461 ttttggacga cgcagatcca aataacgata tttcaaacgt atttcgtcac tgattgcttg
     2521 atcatcttca atttgaaatg gcggggtttt tgcttgattc aaaatcacga tttcacttgc
     2581 aatcacttcg aattcaccag tggccatttt aggatttact gcattttctc cgcgtcttct
     2641 aacttcccca gtcacttcga tcacgtattc actgcgacat ttatccgcga tttcccatgc
     2701 ttctttgttg atttcaggat taaagacgat ttgaacgatt ccttcacgat cacgtaggtc
     2761 gataaagatt actcccccaa ggtctcgtct tttttgtacc catcctttta atgtaactgt
     2821 ttgttctaat tgttgttcgg aaactaatcc gcaatagatc gttctttgtg ccatttattt
     2881 ttttcctcct actcgaataa agtcatttca tcatagactt catcaaaatg ttcatatatc
     2941 tcttctaatg gaaaactttt ttcttttcgg cttgccattg ctttgacatt aatcgtttga
     3001 ctagcaagct catcttcacc gatcaccaat actaatttgg cattcgcttt atctgctgtc
     3061 ttaaattgcg cttttgcttt acggttcatg aagtcgcggt cagctgaaaa accaaagttt
     3121 cgaatcgctt ggaccacttt caatgcttcg atatttgttt cgtctccgag tgcgaccacg
     3181 taagcatcca actcattaaa tgcagggata actacttcct ctgcctccat agtcaagagt
     3241 aatcgctcga tacccattgc aaatccgaag ccaggtgttg aaggaccacc tagttcttcc
     3301 actagattgt cataacgtcc gcctgcgcaa atcgttgctt gtgcccccat ttttggtgcg
     3361 tcactcatga tttcaaaaat cgtatgtgtg taataatcta gtccgcgaac catattactg
     3421 tcgatttcat aaggaacagc caatgcatcc agcatggaaa caacggtatc aaaatgtttt
     3481 tgagcaggct cgcttagata atctaagata gatggagctt ctgccacaaa tttttgatct
     3541 cgtttgtctt tactgtccaa tacgcgtaat ggattttcat gaaggcggcg tttagagtct
     3601 tcactcagct ctgattcaaa aggtaaaaga tagtcgatca atgcttgacg ataggcttga
     3661 cgcgtttctt tatcacctaa tgaattgatg actagccgga tttgcgtgat ccccaactgt
     3721 ttgaagaaat caagtgccat caccatgctc tccacatcag ttgcagggtt ttctgatcca
     3781 aatgcttcta ctccgatttg atggaattga cgtaaccgtc ctttttgagg gcgttcgtag
     3841 cgaaacattg gtcccatata aaaagttttg aacggtttcg catactctgg gccgaacaat
     3901 ttatgctcaa caaaagaacg aacgattggt gctgtacctt ctggtcgaag cgtcacatgg
     3961 cgttcccctt tgtcataaaa atcatacatc tcttttgaaa cgatatccgt tgtatcccct
     4021 acacttcggg atatcacttc atagtgttca aaaatcggtg tacgcatttc attatactga
     4081 taatctctga ataataaacg tgctgtttct tcgacaaact gccatttttc tgattcgcct
     4141 ggcaaaatat catttgttcc ttttggtctt tggaaactca tagttttctc tccctttctt
     4201 agctatgtaa ataaaaaaat cacccttgtt catcatgaac aagggtgaat gaaattcata
     4261 cacggtacca cctaattttg aaagatttct ctttcctctt acgaataacg ctcgtacgcg
     4321 ggacggcttt tcactcgtcc atctccagag tgtctttagg ctaatgaaac tttagatcat
     4381 tttcagcctc gatgatcctc tcttgtaaag tttcaaaaac ttactcgctc tttcattgac
     4441 attcgatatt acttataacc tactaaaaac cagtctcaaa gtcaagagat atctgactat
     4501 ttcccctcac aaaaacgttt tagttcagct ttttggtatt ttgttttaga ctagacaaaa
     4561 aggaggaatc agctatggca gaaataagaa tcggacattc acaagtctac gcagaacagc
     4621 ttggattagg agcaaatgcc gttggaggac ataatctttt tgacggtttg gaagatgaga
     4681 caggaaaaca agtagttcgt acagctttga atagcgggat caatttgatc gataccgctt
     4741 atgcttacgg aaacggccgt tccgaagaac tgattggcga ggtactgaag gaaaaagaat
     4801 atgatcgctc acgtgtcgtc atcgctacaa aagctgctca tgtaccaaat aaagggcgaa
     4861 catttgataa ttctcctgag tttctcaaac aatcggtgga agatgcacta aaacgtctgc
     4921 aaactgatta catcgacatt ttttatatcc actttccgga tgaaagcaca ccaaaaaatg
     4981 aatcagttgc tacattgcac gaattaaaag aagcaggaaa aatccgagca gtcggtgtgt
     5041 caaacttcac gttagaacaa ttgaaagaag cgaatgcaga tggatatgtc gatgtagtag
     5101 aagataaata cagtttgatc catcggcagg ctgaaaaaga attattccct tatcttgaaa
     5161 aaaacaaaat cagttttgtt ccctattttc ctcttgcttc cggtttgctg acaggaaaat
     5221 atgaacttgg tgaggaaaaa caatttggtg aaggagatcc tcgaaaaaga aatccagatt
     5281 tccaaggaga acgtttcaga gaaattttaa ccgctgtcga tgtacttcgc cccattgcta
     5341 aaagatacca agccactcct gcgcaacttg ttttagcttg gtacatgaag aatccacgag
     5401 tgtctgtagt tatacctgga gctaagcgtc cagagcaagt atctgacaat gtccaagctt
     5461 tagatttgca tttatccaat gaagattatc aaaccattga tgaagcattt cgtggatttt
     5521 gatcaattga ccttatcaat ttatagtaat atgaaaaaag gaagatcagc actgtttgat
     5581 cttccttttt ttcgataagt ttatgctaat ctaattcttg taaagtagcg ctcggctatt
     5641 cggataagtc ggaaactgat tgaatggaat cgtccaatct tgttcaggta cagagaactc
     5701 aaacttattt actagctgat cggaaaacac acgtagacta tgaagagtga tccattctcc
     5761 tgcacagcga tgcatttgtc gaaaatttcc cccgccttgt gcaatcatct catattcttc
     5821 tttataggaa atatcttttg ctttccctac ataccgtttg atcataaagg aatctggcgc
     5881 ttcaactgtt cgttcatcat ggtctgttcc atataaatct aaaataaccc aactgccttc
     5941 tggtatacga taaccatcta cttctacttc tttcagcgaa atcgcaggta gcattgggaa
     6001 gaaaggataa tagcgtctca tttcttgaat aaacggatct tgtaacgtcg aaaagtcatt
     6061 ttttaattgc tgatacaaat ctggtctact gaaaagtgca tgtcccatca atgccgccca
     6121 cacagttaga gcaaccgttg gacggatgat gttcaataat tcaacagctg ccacttccag
     6181 aggtaaaagc tgaccatcta aatctgtggc attggcaaaa gcatataatg ccacattttc
     6241 ttttccagcc acaggatttt gtcgagcttc ttcaatcaag ccttgcgccc atttttctga
     6301 ttttttgcga ttctcgacac cttttatgtg atcaatcgga gaagtaaacg ttccgctgat
     6361 catagagatt tgatattctg ctaatttttc gacctcatct ttagaaatcg ctgataaatt
     6421 gatacctgcc cactcgcaaa tagatgtaaa gaaaaccatc ttacttaaat caaatagttc
     6481 aaattgacca tgctgtgctt caagtgcttg tgtcagattc ttatctaaga tacgatgata
     6541 atcttccatt ctttccggtg tcattagatc cataaaaata tttttccgat ggtgatgagc
     6601 agctccatcc aaagtctgta ctccaccttg accaaaaagt gtcttcaata ctaattttgg
     6661 catagcacct tttcgtttga aattccttgg atcataaaat acttttgctg ctgatgatcc
     6721 gtagatcgtg atagcttctt tattgaaaat ctttgctttg acgacgggag cgtccgcttc
     6781 atggcgtaac tcctccaaca tgttgtatcc tttttgatac aattttttta aatctgtgat
     6841 tttgatatct actactggta cttctttcat cttcagcaac ctcctataaa aaataacttg
     6901 tataatttaa gcttaacttt ttttccgact gctgacaaaa gaagcgcttt ttgcagacaa
     6961 aaaagccact taagttagag tttatcagtc taacttaagc gacgtgtttc atagttttta
     7021 ctagtgaaaa ccagattact ttatttgttt ttagaatcaa taatgatcgt tacaggtcca
     7081 tcattgacga gagaaaccga catatctgct ccgaattgtc cggtagcgac tgtgatgccg
     7141 cgacttctga ttccttcatt gaatgattcg tataaaggaa tagctgtttc tggtcgtgcg
     7201 gcttccacaa agcttggtcg atttcctttt ttcgtatccg caaacagtgt gaattgagag
     7261 atacttaaaa tttgtcctcc cacatcttct acactaatat tcatttttcc ttgttcatct
     7321 tcaaaaatac gcatttgtgc aatttttttt accagatagt ccacatcttt ttgcgtatcg
     7381 gtttcatgaa tgcctaataa aacaagtagg ccgtagccaa tttttccaat ttcttgctga
     7441 tcgactgaca cagcagcttt acttacccgt tgaacaactg ctctcatatc tgtcctccta
     7501 tccattcgtc cggcgtacac tgtagacatc aggtactgct ttgattttat ccacgatact
     7561 ctttaggtgg gataaattct ggataccaac cgtcaaatgg atcgtcgcca ttttatcttt
     7621 attcgtccgt gcttcaacgc tgttgagatt tttcgttagc gcattgactg tttgaagtac
     7681 atcattcaat aagcctgaac gattatagcc gtaaatttcc aaatctgcat catactcttt
     7741 tcgtgtattc gatgtatctt cccattcgac ttcaatcaaa cgttctgcta catttggttt
     7801 gtcaggctga acattcggac aatctcgacg atgaatggat atccctctgc ctttagtgat
     7861 ataaccaacg atatcatcac caggaatcgg attacagcag cgactgatac gaattagtaa
     7921 gttttctacc ccttgaatga caacgccacc ttcatgacgt actttcattt tgtcaggttc
     7981 ttttttcggc tgattcatga tttcttctgc ttcttgcttt tgctgctcga ttttttgttc
     8041 tttacgttct ttttcagtca gacgattggc catcgtcaag gggctaactt ctccataccc
     8101 tacagctgca tagagatcat cttctgtttg ataattaaaa cgatcgagtg cttcttgcag
     8161 tttgttcttc gtcaaaatat ctttaggcgt aaatcctaga tctgtaatac atttgaccac
     8221 ggattcgtgg cctttgatca cattttcttc tcgatcttgg gctttgaaga aacgtttgat
     8281 cttatttctt gctttgctag tagcaactaa tttcaaccag tcgcgacttg ggccaaatga
     8341 atttggagaa gtcatgatct caatgatatc tccgtttttc aatttgtaat caagctgcac
     8401 cattttgcca tttacttttg caccagtggt tttgttaccg atatctgtat ggatactgta
     8461 tgcaaagtcc aatggtccgg atccttttgg taactcagta acgtctcctt ttggtgtgaa
     8521 gacgtagact ttatcactaa agatatctcc ttttacgcct tccataaatt cagaagcatc
     8581 atagctttcg tcttgaagtt cgagtatctc atggaaccag cttaattgtt tcgtcatacc
     8641 atctggttct actttttcat tttttccttc tttataggcc cagtgtgcag caaccccgaa
     8701 ttcagcgatt tcatgcattt cctgtgttcg gatttgaatt tctaccggat ttcctgccgg
     8761 accgatcacg gtagtatgca aagattggta catattagct tttggcatgg caatataatc
     8821 cttaaatctg ccgggcattg gtttccattt tgtgtggatt gctcctaata cagcataaca
     8881 atccttgatt gaatctacga tcactcgaat agctagcaag tcataaattt cgttgaactg
     8941 ctttttctga tctttcattt tacgatagat tgaataaatg tgttttggcc gtccatagat
     9001 ttctgcaaaa atccccagct cttctgtcgc tattcgaata tcttcaacag taccacttac
     9061 gtatttttca cgttcttctc tcttcgtctg catcaagtgg acgatacggt aatactgttt
     9121 tggatttaga taacgaagtg ctgtatcttc taattcccat ttgatccggc tgatccctag
     9181 acgatgtgca agaggcgcat agatttctaa agtttcctga gcgatccttc tttgcttatc
     9241 ttctcgcagg tgcttcaacg tacgcatgtt atgtagtcta tcagctaatt tgaccatgat
     9301 aactcgtaaa tcttgagcca ttgctagcag catctttcgg tggttttctg ctagctgctc
     9361 ttcgtgggat ttatatttta ttttccctaa tttggttacg ccgtccacta acatagcaac
     9421 gtcatcgcca aattcttctc tcagatcttc taaagtgatt tctgtatctt caacaacatc
     9481 atgtaagaag cctgtagcca ctgtatgggg atccattttt aaatctgcta atatacctgc
     9541 gacttggata ggatgaataa tgtagggttc gcctgatttt ctaaactgtc cgtcatgtgc
     9601 agcagtcgcg tattcgcatg ctttctcaac aaatgctaca tgttcgggac ccatgtattg
     9661 actaacgagc tttatgacac cgggtcccgt taaaatttct tcttttgtca caggaatcga
     9721 tccccctttc ttttttcggt aaaacgggaa tcgggacaaa tatctgtttg ataattgtcc
     9781 caaaatccgt gcagttatac gtttttttac ggaaatgttc gctttttaaa agctttttca
     9841 aacgattttt cgtatccatt gtataaataa ttgtttctat tatacaaatt gttgactatt
     9901 ttttcaattc tttctgtcga tactctttta ttaatgaaga acgttagctg aaagaaggat
     9961 tcattaattc ccattgatag ctagcagcac ttaataaata aagaggtgct gtttctgtac
    10021 gaaggattct tggtcctaat ccacaaagta tccctttgtg ttctacaaaa taatcgactt
    10081 catcgcggga taatccacct tctggtccaa agacagctaa gattcgacta tctggttttg
    10141 tttcactaag aattttggct gcatttgatt tttcgccttg ctttgctgat tcctcataag
    10201 caaccaagac cacgtcataa tcatctagcc gctcaaataa atcttgtttc gtttccaata
    10261 actctactga tggcacgatc tgccgatggg attgttccgc tgcctcttga gcaatctttt
    10321 caagccgttg ttgtcgtttc atacgttttt ttgcatccca tttaacgaca gaggctgatg
    10381 ccgggaaacc aagaaaagct tgcgctccta attcagtgga tttctgaacg atccaatcca
    10441 gcttttcccc tttaggatag ccactcgcta tggtaatcga acttggcagt tccttttgat
    10501 tttcttcttt tttaatttct ttcagataaa cagcctcttc agtaatctcc gtgatttctg
    10561 caacaatggc tatttgatca ttgaatgcca aaaaaacttc atcctcgggt tccatcctca
    10621 taacccgtac aatgtggtga tagttttccc cttctaaaac aaaatagtct tttgattcat
    10681 atgattcttt taaaaaatac cgctgcatct attccacctc tggttttttc aaaataattg
    10741 cataccagtc tttttgctgg aaaatttgat ccacgataaa cccttgttct aacatggcat
    10801 ccagaaccat ttgcttcttc tcttcaatga ttcctgaaac gatcagcgtg cctgtttgct
    10861 tcagtaaacg ccaagcatct gggatcatca ataagatgat atctgctaaa atgttcgcaa
    10921 cgattacatc actttcaatc tcgattcctg tcaaaagatc gttagctgat acatggacat
    10981 ctttggctat ttcattcata tccatatttt ctttcgccga acgaacggct acttcgtcta
    11041 aatcaaaggc atagacatct tttgcaccaa gatattttgc tgcaatactt agcacgcccg
    11101 acccagttcc cacatctagc aatgtttcac caccacgcaa gactgtttct agtgctgtca
    11161 atgtcaagga agtcgtggga tgagtgcctg tgccaaatgc cataccagga tctagtgtga
    11221 taattttttc tagaggatct tctgtctggt agttttccca gcttggcgca accgtcagca
    11281 ttcgcgtaat acgcaccgga tgatagtatt tcttccaggc tgttgcccag ttgctttcct
    11341 ctacttcact aactgttact ttattttttc caatagctag accaaactca ggtaattgtg
    11401 tgatacgatc tttgataaaa ggcaagattt ccggtaagaa agtcgtttcc ggaaaataag
    11461 ccatgactag tgcgccttct ttcagcgatg taaaatcatt tttatctaaa atctcaccat
    11521 attgatccga ctgaaaatgt tcaacatcta aagaatcttc aatcgcgaca ccactcgccc
    11581 ctgcctccat caaaatatta gcaactgctt cgactgcttc actttcagtt tctactttta
    11641 cttcattcca tttcatatct gtcactcctt aataacttgg gtacggtaaa taagtatctg
    11701 cttcattttg ctgttttact tctactaatt ctgctagttg ttgattatcc cagtgaagct
    11761 cgaatacagc ttcgtcatcg tcttcatcca aaaaatcaag caaatcgctt tgcccttgag
    11821 ctaatacttc atttagatat tccactaaag aatcagctac tgattttgcc aacccttttt
    11881 ttccttcata agggattact gctagataat tgcgttcatc aaaaacagat ttctgaggat
    11941 taaaaagaag tacgccgtct tcaaattcga taattggttc ttgcgaaacg atcccttctg
    12001 catcatcaat ctcaaattgt gctttatttt ctgcaaacaa gcgaacaacg atctcgatcg
    12061 tatgattttt tgtatcccaa tctaaagcta catcatagtc tgtcacttgt ttttcaatta
    12121 tttcatctaa atatgttaat agatccttct ttttcatttt ctttcctcca accccaatct
    12181 ttctcgattt ggttttttct ttgtaaagag atataaggat ttcccgacac aacaaaacgc
    12241 aacggctttt ctgtccatac acctttgttt gggataccga ttctaggcaa agcattgatc
    12301 ttttttggtg tctttttttt ctcaaaaact aagcgtaacg aactatctgc tatagactga
    12361 ccataaagtt cttgaggaat tgctaatgct tccactaatt ttcccggacc gttgctgata
    12421 tctggaccag tttttccgcc acgattttta ctcatttgat caatcattgc ggcgggttcg
    12481 attgcacgta tcattactcc ttgaggaatc ccttctggct gagtaatgat gttcaaaatc
    12541 cgatgagtat gcatcgtgta aaggtagatc gtacctggtt tttcatacat tgcccgcaca
    12601 cgcggtgttc ttcttaaacc atagctatgg gcggcctcgt cttctggacc taggtatgct
    12661 tccgcgtcta caatatatcc agctaatctt ccttctggtg tgtcatgctc caaatacatc
    12721 cccaataaat cccgtgccac ttctggcgtc gttttattcc aaaaaaattc atctatcttt
    12781 tgtttatccg tcatttttct cacctacttt atcatacaca atattagttg gataagctat
    12841 gaaaacaaaa caacagaagg ctgttctttc taggcatctt gctaaatatg atataattaa
    12901 gggacgtaaa aacaagaagg aggaaaatcc tatgcaacag ccattggctt atcgcatgag
    12961 acctcgaacg atcgatgaag tcgttggtca gcagcatcta gtcggggaag gaaaaatcat
    13021 acgccgcatg gtcgatgctc gcatgctctc ttccatgatt ctatatggac ctcctggtac
    13081 tggaaaaacg agtatcgcaa gtgcgatcgc cggctctact aattatgcat ttcgcatgct
    13141 aaacgccgct acagattcaa aaaaggatct acaagttgta gcggaagaag caaaaatgag
    13201 tggtacagtg atcctcttat tggatgaagt acaccgatta gataaaacaa aacaggattt
    13261 tctgcttcct catttagaaa gtggccgaat catcttgatc ggagcgacta ctgagaatcc
    13321 ttatattacc atcaatccag cgatccgcag ccgtacacag atctttgaag tcaaaccatt
    13381 aacagaacaa gacatccagt tggcagtcga acatgcttta agggataaag aacgcggcct
    13441 tggacaacaa gcaatccagt tagacgaaga tgctcttctt cacctttcgc gtgcaacgaa
    13501 cggcgatttg cggagtgcat tgaatgggct ggaactggct acactatcga ctccttcaga
    13561 taaagaagga aggattcatc ttacactgtc gatcattgaa gaatgtgtcc aaagaaaagc
    13621 tcttacccat gacaaaaatg gagatgctca ttatgacgtt atttcggctt ttcaaaaatc
    13681 gattcgtgga agtgatgtgg atgctgcact tcattatctc gcccgtttag ttgaggcagg
    13741 tgatctggca agtatttgcc ggcgtctgat ggttatcggt tatgaagata taggtttagg
    13801 taatcctgct gctgcggctc gtacagtgaa cgctgtatta gctgctgaaa aattgggatt
    13861 gcctgaggca cgtatcccac tagcagacgt cgtcgtcgat ctttgcttgt cgccaaaatc
    13921 caattctgcc tacatggcat tagatgcagc acttgctgat attcgagagg gcaaagctgg
    13981 agatgttcct gatcatttga gagatagcca ttacaaaggt gcgaaatcac tcaaccgtgg
    14041 cgtgggttat cagtatcctc atcattttga tcaggcttgg gtcaatcagc aatatttacc
    14101 agataaactg aagaatgctc agtattatca gccaaaagat acagggaaat atgaacaggc
    14161 tctgggtcaa cagtattacc gtataaaaga gtggaaaaag catcctccaa aaaactagac
    14221 aagaaaacgg ttctgtttct gcgagctctc tcattgctct ttttaaattc atatgctact
    14281 atagaggtgg gaaatctgtg atgttcgaat tgtcctcttt gtgtgttgac cgaacatttt
    14341 tattgatatc ttgggacccg tcttgtgtca ctgctggtaa catgccttat catttggtag
    14401 ttcgaagcta cgaccatggg acccacctgc ttgaattcgc gggatcaata ctacaggaca
    14461 aacaacggca ttaacggaga ttaccatctt ttaaagaata aagcaatttg ctcttggcaa
    14521 attgcttttt ctatttgttg cttattttgg aggatttgtt catgattagg attttaatga
    14581 ttatcgccac tttagtatta ttattcgtta gctattattt atttaataaa caggatatct
    14641 tttttgtatt gatcaacaaa aatgataaaa atcaaggatt cctacaattt tacggtgctg
    14701 catatgctgt tttaggcgta atgggtattt taacagcttt tttcaatcaa cgctttattg
    14761 ccttggtctt tttactgatc gtaattattg tttctgccac cttcagtatc cgatttgcaa
    14821 aaaaaattgc tgaaccaaag cagtaaataa ttctcttttt ccctaaagtg ttctacaata
    14881 aaagagtaca atacgattga cttgaggagt gaatgcttat gttacaacaa tacaaaaaaa
    14941 tcatggttgc cgtagatggt tctgatgaag ctgaattagc ttttaaaaag gcggtcaatg
    15001 tagctatccg aaataacgga gaacttctat tagcacatgt cattgacaca cgttctttcc
    15061 aaaccgtttc atcctttgat ggaatgcttg cagaacaagc tactgaaatg gcaaagcaaa
    15121 cattggcaga ttatgaaagc aatgcgaaga aagctggctt gaataatgta acctctgtcg
    15181 tagaatatgg ctcaccaaaa caaattattg ccagagaaat cccagaagat aatcaagtag
    15241 atttgattat gttaggagcc acaggattga atgctgtaga acgtttattt attggttctg
    15301 tttctgaata tgttattaga aacgcagctt gcgatgtgct tgttgtccga actgatttag
    15361 aaaaccaatt gccagctaaa gaccacacca aagcttaaca cgtagacgac ctgccaacta
    15421 tcggcaggtt tttttgcaca aaaaaccgtg ataaaaacaa aagacgcggc aagcactcat
    15481 cggtctttat aatgtttaaa ataaagggaa aacagtcaat gagttcaagt aaaataaaaa
    15541 aagtgaacac cagggcgatt agtcgccctg gtgtggttta aaacgaccta agcatgccaa
    15601 agcttattgg ttcgtcaatg tggaagtttc cgatgaatct gatacgaaaa aatatgaagt
    15661 atcgatggcg gataccgatt ttatcgccaa actaaaattt tatttcgggt tcgggtaccg
    15721 cgagaaggca agtgctacat tcggtaaatc attcaagaat tgatgaaagg ctaccgcttg
    15781 ccattgcaac cacaacgata ctcctgaacg cgatgttggt gagatttcca aagagaagag
    15841 ctgtttaatg caacggtatt aagcaaatgg caaaaggaag tcatgtagac aaagtttttt
    15901 atcaaacacc atatgatttc gcctgaacgc tggtttagct cagaatacaa tgatgtggtc
    15961 cacgagacat taccctcagt cagataaaaa aagctctctg acagaattac acatcgataa
    16021 atattaagta aatgtcagcg ctagttgaac tttctgacac tttcaggtac actggaaaag
    16081 acaactacac gaaggaggga aaatactatg atgcgcatga ctaaaataac aagtaatgaa
    16141 aattaccaac gataccatat cgcatttagc catgcaattg gttttacagt tgctgttgac
    16201 gggacaagtt tgtccaaaac agttgaattc acgtaaaatc aatggccatc tcaaggtatt
    16261 tttctcatga taatctgagt ttaaataaca caggccaggg ataaccatgg cctttttgag
    16321 tggtctcaag gagagagatc atgatcatac aagcaatttc tattaaaaaa acaatcggcg
    16381 ggaatccatt atttgaggat ttatcctgtc aaattgaagc tggtgaaaaa attgccttag
    16441 tcgatatcaa cggaactggc aaatcaacct ttttacaaat cttgaccggc agagaaggtg
    16501 tcgacagcgg tgttatcagt cgcaaaaaag gcttaaagat cgggacggtc gaacaagaac
    16561 ttacagtttc cgaagcgacc gtcaaccact acttgctcca ttctgccgct gagatacagg
    16621 acctcaaaca gcaaatgagt cgctacgaag cattgatgac agaaccagat acaaacttag
    16681 agaagtgtct ggcccgttac ggtgagctgc agcatcgctt tgaagaacta ggcggttacg
    16741 tattagaaga ccggattgca gcgatcttgc aaggcttagg gattccacac tgtcaggaag
    16801 cacggttaac agaacttagc ggaggcgaac gagttaaagt cgctttagcc aaaattttag
    16861 cagccgatgc cgatttactt ttactcgatg aaccgaccaa tcatttagat cttaacagta
    16921 ttcgctggtt ggaaaactat ttacagaata caaaaaaatc ggttctggtc gtttctcatg
    16981 atcggcaatt tctcgatcaa gtcaccacaa aaacatggga actggaagaa ggtgcattga
    17041 ttgagtatcc cggaaactac agccgttttc gagtcttgaa agaagctcgc ttggctgagc
    17101 tgacaaaaaa ttacgaactg cagcaaaaag aagtccagcg gttgaaagtc atgattcgtc
    17161 gttttcgtca gtgggcacat gaaggagaca atgagtcctt cttcaaaaaa gccaaagaac
    17221 tggaacgccg cctggcgaaa ctgacactcg tcaaaccgcc acctcccccg aaaaatcggc
    17281 tgcaaagctt gtctaatggt ggaaaatctg gcaaagaagt tttcatcatt caaaacttgc
    17341 accagcagta cgctgatcaa gtactattca aagacagcag ttttgcagtc tatcgtggag
    17401 atcatttagc catcatcggc gacaatggtg caggaaaatc aaccttactc aaattattac
    17461 tcggtgagga aaagccttac ggtggcacca tcaaattagg tagtagcttg caaatcggtt
    17521 atttgccgca gcagctgcag tttgccgatc ccgatagccg tttgctggct tacgccatca
    17581 cattgactgg aaacgaagaa gtcgctcgac ggcaattagc caaaagtggc ttttatcaaa
    17641 gcgatgtggg taaacggatc aaagatttat ccggtggtga aaaaatccgt ttggcgttgc
    17701 tgaaactctt tttagaaaaa atcaacgtcc tgattttgga tgaaccaacc aatcacctcg
    17761 acagctatgc tcgagaagaa attgaagaaa tgctgcaaga ctatcaagga acgctcctag
    17821 cagtcagcca tgaccgctac tttttgcagc aacacttcca agaagcactc gttattgatc
    17881 aggagcagat cagccgccag ccgctcggcc aactgattga ctaggacgtt ttttcgcaac
    17941 aaaaaacttg attttcatac ctgtgctttc acgattcagg caaagtacag gtggaaaatc
    18001 aagtttttat tattcgatca atgaatgttt cggatcggtt ttctagctaa acataaagcg
    18061 aagaataaag tcgacagccc acgaggctga tctaaaaatg ttttttcttt ttacatatgt
    18121 aatgtccctt ataaagatga atgaaaataa aatattggga ttttcctgaa aatcaataaa
    18181 taaaatgcca gtcttttccc ttttgaccac ttttccaagc aaaattccct aagaagatcc
    18241 tgtaaaaaag aagggaaatt gaagttaaat taccattttg cggtcttctc cgcaaaaaaa
    18301 acacatgcaa gaattccctc ttgcatgcgt ttcatctttt atcctaacga atgctttggc
    18361 ttagccgcgc agacgctcca catcacgggc aatcattagt tcctcatctg ttgggatcac
    18421 taatactctt acttttgatt cttttgtaga gatatctact tcttcaccac gcaattcatt
    18481 caacgctgga tcgatttcac aaccaaacca agacatacct ttgatgattt cactacgtac
    18541 atgtccgtca ttctcgccga taccagcagt aaagacaatt gcatcaacac cattcatcgt
    18601 tgtcacataa ccgccgatgt attttcggat gcggtccacg aaaatgtcat aagctaaacg
    18661 aatatgttct ttttcatagt tcttttcaag atcacgcata tcactagaca atcctgttag
    18721 gcctagcaga ccagatttct tgttcaaaac attgatcatt tcatcaatat caatctctaa
    18781 tttttccatt aaatatggga gaacagctgg atcgatatca cctgaacgtg tacccattgt
    18841 caccccagct aatggtgtga atcccataga agtatccatg gatttacctc catcgactgc
    18901 tgtgatcgat acaccatttc ctaagtggca agtgatgatt tttagttctt cgatcggttt
    18961 gttcaataat tttgccgcac gttccgacac ataacgatga cttgtgccat ggaagccata
    19021 ttttcttact ttgaaatctt cataataaga caccggtaag ctatataaat aattttgctt
    19081 tggcattgtt gagtggaaag atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat
    19141 ctttttaaac gctttgatcc ccattaagtt tgcaggatta tgcaaaggag caaattctgc
    19201 taattcagca atgcggtcca ttactttctc atcgatcact actgaatccc cataagtttc
    19261 tcctccgtga acaacacggt gccctactcc ggtgatttca tcataagagc ttaaaatttg
    19321 cagttcaatc aactgatcta acagcatttt tactgctact tcgtggtttt cgatatccat
    19381 gatttgttcg aatttttgat tatcgccgta cttgatggta aaaatagaat cttgtaaacc
    19441 gatacgttcg acgatccctt tcgcaactac ggtttcttct ggcatttgat ataattgcca
    19501 tttcaaactt gaactcccag cattgattgc aattgtttta gacatatgac tctccttctc
    19561 gttgaatgat tacagagcag atgatttcca gtttttaaac tcgttgaaaa attccgtcac
    19621 cttctctggt tcttttaatg aagcaagttt cacaagcaag acttctttta cttgttgtgc
    19681 ttgtggtcct tttttctgta agatcaaaat acttttttgc gattgtttat tacggaacaa
    19741 ttcatctggc aattgaatca ttccttgaag ataaccttct tctttaaacc attttttgat
    19801 ttcttcactt tgatccgttt ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc
    19861 cggcttcaca tatttcattg attgctctag caataaatga tgtgcataac tatggccttc
    19921 ttcggtactt gttaaaaact cttttgcttt ttcgtcattc ggataataac cgattggcaa
    19981 atcgctgatt gcaaaatcga caggatctat taataattcc tgcaacccgt cttgatgaaa
    20041 gtattgaaca tttgcttgtg tcaaatcact tgtagaagct gcgacagcca acaatgtatc
    20101 atcaatatcc acaccaattc cttctgtttg gtaccccgca ttcgataggt ttaaaagaac
    20161 tgttaataac aagttgccca ttcctgccgc aatatccaga atcttaaccg gtgctttttt
    20221 atttgtgtat agttgttcaa taagaaaaac aaataaaaaa ccgatactat caggagtgag
    20281 ttgatggttc gcctgcaaat gttctgtctg gctgcccttc aataaaagta attgagacag
    20341 tctgcgccat tcttctggct cgaaagaaag tttcttcaac tcttcataaa gagctgtgat
    20401 ccgttgcgtt gtctcttttg tcggcactcc atccaccaca cgtacttgat aatcatctat
    20461 taaattttct gcattttcga cataggcttc taaaaaggaa gttcccaatg cattctgtaa
    20521 aagctgaata gcttccaggt tttgattaaa cccttgttcc attttttctg gtaacatggt
    20581 gcacctcatt tctagatata ttataacaat tttttcacct gtttaattct atcgaacaat
    20641 tgacaaaatt tcaatcgttt tcactgtttt ttatttgtga aacggcaaac atttttttat
    20701 taaaaacagt gttataacag aaaagagggc gttttcacga acattccatc attctgtact
    20761 agtatctgta tcatttttta cttcttctgt gaaccgaaag ataaacggtg agacttccac
    20821 ctctaagaat agggactgtt gtacccactg atatgttaca gatccagttg tgtagtaaaa
    20881 aactccttcc gaactttcgg caagcgtaga ctgttccgat aaagccatct gcttcattat
    20941 ttttgctata taataatttt tgattcgtat attaagatcc attgacaaac ggtagctttc
    21001 taatgctgcc atcaataaaa acgtacataa aagaaataaa agcaagacag aaaataaaat
    21061 agagccctta taacattttc tactgatcgt tttttgtcca tatggcatat ccatcttctc
    21121 cattttcaaa gtgtacataa aaagaaattg tgtgttcttt ttccaataat tgaagctcag
    21181 tgatacttgt taacataggt tgatgtccgc cggaagttct tcttttacgt atcattgcct
    21241 gatatctttc gatgatatac tgtttatcct cgatcgtgta gtgcagttgc tgatttttca
    21301 cagagacatt gtgactgctc ttgagttctt cttctaattg tgctaaaaat attagccact
    21361 ctttttgatc tttacgctgc aaaaattcac ttacttggat ggtttgctga atcaaaagat
    21421 tgaatgtgag caatagactg ctcaatatca tcagtccaat tagacattca atcagcgtaa
    21481 atgctggaat tacttgtttt ttcaatactg atcgcttctt tctccatatt gacaaacact
    21541 ttttcgatta ctccattatt atcgacaatt tggatatcgt atgggatcat ttcttccctt
    21601 cgctctggtc ttttgtgata catccgctgc gtcttgatat cttcataaat cattcgaata
    21661 aatagcagtt cctccccact tttcttttct gcctgaagca aaaacagctg gccaaagctc
    21721 actaagtaga cacagacaga agcgatcata agcgctgtaa gggactccaa caaaacaaat
    21781 cctgttttat tcaattcttt ttgtatagcg accactccct atctggaatt gatacgtaat
    21841 cgtccgctct ttttcttccc aataaaattg ataagctttt agtgaacttt cattcccggt
    21901 tctagcagca aaagtaatag aagcatggcg tttcaaggta atctcatcag gaatatctaa
    21961 tacttcccaa tttattttat ctggattcgg aacatgaaaa atgatttgat tctcttcact
    22021 attccaataa aagcctgttt ggatctgatt cacaatagcg attttttgcg tagaatagat
    22081 tcgtttttca aattggccga aaaattgata gactgccaat tcattcttcc atgcagaaaa
    22141 tgcgagaact ggaaaagaaa ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa
    22201 caacgtatat cctgatgcta aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt
    22261 ctcgtttgat atagccttca ttcaacaatt cctccacagt aggaactctt ccattatttt
    22321 gaagcgcata gagctctttt tgtgtgtcta ccaactggat gatcgcttca cgactttctt
    22381 ggtctacatg attcctataa cgcgacaaat tagggacgaa caacaacact aatacactga
    22441 tgatcagtaa gacgattagc atttcgatca atgtaaaacc gctataataa gatatttttc
    22501 tctttttcat ggcaaaacct cctctatgct tccgtaaaca ggtaataaaa cagcggcata
    22561 aactaataaa atcaaagccg ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca
    22621 gtgatgaaga cgtttgaaaa gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt
    22681 acctaaactt ccctttgcct ctccctgtaa aacaatttta ctgaattctt ctgatagaaa
    22741 acgataattc tggaactgct cagccaatga catacctgcc tccaatcctt gaatcaactt
    22801 agaggcaaga tgctgaatca gactttcttg tttcgtttcc tttaagcaga ctatgatttg
    22861 ccttaattcg aaaccttcat gaaaaagctt tccaatctct agtgacaaat aagccgattg
    22921 atacaaggaa tatatcggtc caatcaataa ttgtctgctt atccattcac tctgatcaac
    22981 aggagacaac ttcatcagac gataccttac tagtaaagcc aatagagtaa ccgctaggat
    23041 gaatagtaga acataccaat gaaagacttg tatcagctga ataccccaat gctcttgcat
    23101 gaccattcct gaaccaataa gctgaggcaa aacgaacaac cgcatactaa acaagatacc
    23161 tagcaaaaag gtgagcaata gcatcggata tgcaagcagc tttttcaaat cattgcgaaa
    23221 tccttcaaca agccgaaact gtttggcaat ttccaacaaa gttgtcggca gattcccatg
    23281 acgttcagct aactcgatct gtaccagttg ctctgctcca tatcctattt gcgaaagtac
    23341 ctcactaaac gcttgccctt cctttagctg atgttggata tttgccaaaa taacaggtgg
    23401 aaacgaacgg atcgtgagta ataaataaag ggattcttgc agactaaaac cagataaaag
    23461 cagttctccc atggtttgtg caaactggtt ttgctggctt cttgttaatc ctttagaata
    23521 gttctgactg ccaaatattt tcctgagctt cttcataact ttcctcccat gattttttta
    23581 ctgattcttc ctccataaat tgataactcc ataaaacaga aggatggtga ttggcatctt
    23641 ccaaaagttc ttgataaatg acaccagcta aacattcaga tagctcttct tttttctttg
    23701 tcagttcatg gattctgacc aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa
    23761 cccggtgccc tgttagtgct gctcttaccg cagcatgtgc agttgattca tctcttatct
    23821 cgccaatgat tagcaagtct ggacgatgcc gtaatgcgag tttcaaaagt gtatcataag
    23881 tctgtgctat tttcggattg atctgtagct gcagaaaaaa cggttcttct atctctacag
    23941 gatcttcgat agtaatcact tgcaattttt cttccaaagc caaccgatac attagagaag
    24001 tcttgcctga tccgactgtc ccgctgaaaa ggtataatcc acgtctgacg gttttctgta
    24061 aaatagttgc aaaatctcga gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata
    24121 atagccgaat cactaaactc tctcgttgaa ggtaatctcc tacagaagac aaccgcagac
    24181 gaacttcctg tttttctaaa gaataagtaa atgcacctaa ctgagctttt cttttttctc
    24241 cgatatccat ctgtcccaaa tacttaaatc ttgagatcag ttgctgtcca tctgccgaag
    24301 agactttctt ccagaatgtt cgtttttttc catagcgaaa atagatgtaa aatacttctg
    24361 aagctggcaa tatatatacg tcttgggcat gtttggcaaa actttcactt atcatttgat
    24421 ccgataatcg ttttatttcc attattctcc cctccacaaa aaacatacgt ctttttgatc
    24481 tttttgtttc aaataaaatg aatttacaaa aaaatattgg caaaagccga atttatgtta
    24541 tacttataaa aatttatctt gggaggcact ccattggaaa caacattaag gaaatatatc
    24601 gaagtagcaa gtaaaaaaaa gcctgctgac ctagtgatca agcatgctaa gatcgtcaac
    24661 gtctttacga aagaaatcat ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga
    24721 atcggtgaat acgaaggaaa aagcgtacat aatgctaatg ggcaatatct agtgccagga
    24781 ttcatcgatg gccatgtgca tatcgaaagc tcacttttat caccaagcga attttcaaaa
    24841 gtatccttgc ttcatggagt tactactgta gtgacagatc cccatgaaat aggaaacatt
    24901 gctgggagta caggtcttga gtttatgata gaagatgcaa gacaaacact catgaatatc
    24961 ttttttatgc taccttcttg tgtacctgta accccttttg agaccaatgg tgcaaattta
    25021 aatgcaaaaa aattagcccc ttttttacaa aaacccgaag ttttaggctt agctgaagtc
    25081 atgaactatc aagcagtagc aaataatgaa acagacatcc tagaaaagat ccaattgatg
    25141 caccagcata agaaaaaaat tgacgggcat gcagctggta tcggcatgga agagttaaat
    25201 gtttatcctg cagcaggtat tcggacggat catgaagcta caactgcaaa agaagctaaa
    25261 gaacgtttgg atcttggtat gtatttaatg gttcgagaag ggaccgttgc aaaagatctg
    25321 gcatcgctac taccggcgat cacacctgaa aattcaagaa gatgcttctt tgtcaccgat
    25381 gataaactga tcaatgattt agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct
    25441 attgcacttg gaatcgatcc attacaagcc attcagatgg caacattgaa tgcagcagaa
    25501 tgttttgatt tgaaaacact aggtgcgatt gcccctgggt atcaagctga tttcttctta
    25561 acagacgatc ttaccaagct gccaattcat gaagttttct ataagggaac attagttgtt
    25621 caatctgggc aactccaaga ccatttattt gctgatcatt caaattcata tacgtgttgt
    25681 ctgccaaaac taaacgctca accattaaat ccacattcgt tagagctgct tctttcttct
    25741 tcacaagccc atgtgatcga aattatcccg aatagtctag tgactaaaga tttggtagaa
    25801 aaagtggacc gacaaggcga acgattcatt ccttcaatcg aaaaagacca attaaagatt
    25861 gcggtgatcg agcgtcacca tcaaacggga aacattggat taggcatagt gaaaggattt
    25921 cagctgaaac aaggagcgat agcgacaacg attgcccatg actcgcataa tctagttgtc
    25981 gttgggacaa acgacgagga tatgctgtat gctgctaatc ttttgattca aaaaggtggc
    26041 gggatgaccg ttgtcaacaa acagcaggaa atagcttgtg tttctcttcc tataggcgga
    26101 atcatgacac aggaaccttt ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa
    26161 gcacatcaac taggagcagc taaaaccttt gatccttttt tgacactttc tttcttaaca
    26221 ttatctgtca tacccgaatt gaagatcacc gacaaaggat tattttcatt ttctcgcttc
    26281 caattgattc caccttgtgg aaaaaccgat gaaataaata cattttaa
//
LOCUS       DAPCMU010000023        25223 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPCMU010000023 DAPCMU010000000
VERSION     DAPCMU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.023"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            128..775
                     /locus_tag="RD744_000814"
     CDS             128..775
                     /locus_tag="RD744_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDU0556842.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    904..1120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1168..3813
                     /locus_tag="RD744_000815"
     CDS             1168..3813
                     /locus_tag="RD744_000815"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDU0556843.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4123..5436
                     /locus_tag="RD744_000816"
     CDS             4123..5436
                     /locus_tag="RD744_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDU0556844.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5426..6079
                     /locus_tag="RD744_000817"
     CDS             5426..6079
                     /locus_tag="RD744_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0556845.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6134..6685
                     /gene="radC"
                     /locus_tag="RD744_000818"
                     /pseudo
     CDS             6134..6685
                     /gene="radC"
                     /locus_tag="RD744_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6816..7901
                     /locus_tag="RD744_000819"
     CDS             6816..7901
                     /locus_tag="RD744_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0556846.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7898..9790
                     /locus_tag="RD744_000820"
     CDS             7898..9790
                     /locus_tag="RD744_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0556847.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9797..10174
                     /locus_tag="RD744_000821"
     CDS             9797..10174
                     /locus_tag="RD744_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0556848.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10328..11110
                     /locus_tag="RD744_000822"
     CDS             10328..11110
                     /locus_tag="RD744_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDU0556849.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11236..11967)
                     /gene="erm(A)"
                     /locus_tag="RD744_000823"
     CDS             complement(11236..11967)
                     /gene="erm(A)"
                     /locus_tag="RD744_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDU0556850.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12025..12084)
                     /locus_tag="RD744_000824"
     CDS             complement(12025..12084)
                     /locus_tag="RD744_000824"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDU0556851.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12476..13138
                     /locus_tag="RD744_000825"
     CDS             12476..13138
                     /locus_tag="RD744_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0556852.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13373..13516
                     /locus_tag="RD744_000826"
                     /pseudo
     CDS             13373..13516
                     /locus_tag="RD744_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13666..13866
                     /locus_tag="RD744_000827"
     CDS             13666..13866
                     /locus_tag="RD744_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0556853.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14120..15799)
                     /locus_tag="RD744_000828"
     CDS             complement(14120..15799)
                     /locus_tag="RD744_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDU0556854.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15801..16013)
                     /locus_tag="RD744_000829"
     CDS             complement(15801..16013)
                     /locus_tag="RD744_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDU0556855.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16406..18136
                     /gene="efrA"
                     /locus_tag="RD744_000830"
     CDS             16406..18136
                     /gene="efrA"
                     /locus_tag="RD744_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDU0556856.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18133..19893
                     /gene="efrB"
                     /locus_tag="RD744_000831"
     CDS             18133..19893
                     /gene="efrB"
                     /locus_tag="RD744_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDU0556857.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19988..20185
                     /locus_tag="RD744_000832"
     CDS             19988..20185
                     /locus_tag="RD744_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556858.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20338..20823
                     /locus_tag="RD744_000833"
     CDS             20338..20823
                     /locus_tag="RD744_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDU0556859.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20927..21520)
                     /gene="clpP"
                     /locus_tag="RD744_000834"
     CDS             complement(20927..21520)
                     /gene="clpP"
                     /locus_tag="RD744_000834"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDU0556860.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21700..22227
                     /locus_tag="RD744_000835"
     CDS             21700..22227
                     /locus_tag="RD744_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556861.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22318..23055
                     /locus_tag="RD744_000836"
     CDS             22318..23055
                     /locus_tag="RD744_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDU0556862.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23059..23976
                     /gene="miaA"
                     /locus_tag="RD744_000837"
     CDS             23059..23976
                     /gene="miaA"
                     /locus_tag="RD744_000837"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDU0556863.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23978..25207
                     /gene="hflX"
                     /locus_tag="RD744_000838"
     CDS             23978..25207
                     /gene="hflX"
                     /locus_tag="RD744_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDU0556864.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8397 a         4109 c         5302 g         7415 t
ORIGIN      
        1 cgcagatgca agcttttgaa caccgtttta tttgtctcac acccttttcc ttcctacttg
       61 taaccttgct cccgtgttag aaacatgtta cactaaatat gtgaaattat taacaaggag
      121 cgtcgttatg cgagaaaaaa cagatacact gccaaaagca actgcgaaaa gattaccttt
      181 atatttacgc tacttgaaga tgctggatga ctcaggtata agtagaatca aatcaaatga
      241 gttcagcgaa atcacccaaa ttccttctgc aacgatccgc agagacttct cgcaattagg
      301 cgagctaggc agaagcggat atggctatga tgtccctttt cttatcgatg tattcaataa
      361 tatccttaat actaaagaag aaaaacgaat cgcattagta ggatatggaa atcttggcaa
      421 agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga
      481 taatgatcca gcactgatca atcgagtaat tgatggagaa atgatctatc cgattgatcg
      541 tttcgcggag atagccaaag caaaaaatgt gacagtagca atctctacgg taccaagtaa
      601 atattcacaa tctgctattg atgaaattgt caaaggaaat gtaaccgcta tacttaattt
      661 tgcgcctgac agagtaactg tcccagctta tgtcaatgtt caatacattg atttgacaac
      721 cgagttgcag acgcttattt actttgatga gaattactca gaagtttttt cttaggaaaa
      781 tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt
      841 actaaatgaa gtgttttttt cacgaccact tgttgactaa aaaaaaaaac cattgtagaa
      901 tgaagcataa cgctgaacaa gaggagtaac tggcacattc attcttagag agagaactat
      961 ctgctgaaag gttctcaatg acaaccagtg aaggtagctt gggagtttct gtcttgaatg
     1021 gagtagagat agacgagtcg tgatcgttaa ctcacttgag aggcaataat gattgattat
     1081 tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta ttttaggacg
     1141 ttttttagtt taaaggagga aaacagcatg acggaagaaa ataacctatc aacaaaatat
     1201 aatccgcaag aagttgaagc tggacgttat cagcattggt tagaacaaga tttgtttaaa
     1261 ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc aaatgttact
     1321 ggaaaacttc atttagggca tgcttgggat acgaccttgc aagatatgct gattcgtcaa
     1381 aagagaatgc aaggatatga tactttatgg ctgcctggta tggaccatgc aggaatcgcg
     1441 acacaagcaa aagtagaagc gaagcttaga gagcaaggga tttctcgtta tgatctaggt
     1501 cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag agtatgcgag ccacatccgc
     1561 gaacagtggg ctaaaatggg cttatcttta gattacagta gggaacgttt tacattagat
     1621 gatggattat ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa aggtttgatt
     1681 tatcgtggtg aatatatcat caactgggat cctcaagcac gaacagctct atctgatatc
     1741 gaagttattc ataaagatat cgaaggcgcc ttttatcata tgagctatga actagcagat
     1801 ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg agatacggcg
     1861 attgctgttc atccagaaga cgagcgttat caagcgctca tcggcaaaaa agtgattcta
     1921 cctttagtca ataaacaaat cccaatcatt gcagatgaat atgtagatat ggaattcggg
     1981 acaggtgtag taaaaatcac tccagcacat gatccaaacg actttgaggt aggtaatcgt
     2041 catgatcttc cacgcgtaaa tgttatggat gatgatggta cgatgaatga actagcagga
     2101 aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa
     2161 atcggtcgat tgattaaaat cgatacgatg atccacagtg tgggtcattc cgagcggaca
     2221 ggtgtcgttg ttgaacctcg gctgtctact caatggtttg taaaaatggc gccgttagcg
     2281 aaaaaagcca ttgataacca agaaacagat caagcagtgg aattcttccc tccaagattc
     2341 aatcaaacgt ttttacgttg gatggaaaat gtccatgatt gggtaatctc acgacaatta
     2401 tggtggggac atcaaattcc tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg
     2461 atggaagcac cagcggatag tgaaaactgg gtacaagatc ctgatgtttt agacacttgg
     2521 tttagttcag ctttgtggcc tttctcaaca atgggctggc cagatacgga aagcgaagat
     2581 tataagcgat acttccctac tagcacttta gtaaccggtt atgacatcat tttcttctgg
     2641 gtaagcagaa tgattttcca aagcttggaa tttactggcg aacgtccatt tgagaatgtc
     2701 ttgattcatg gattgattcg tgacgaacaa gggcggaaga tgagtaaatc attaggtaat
     2761 ggaatcgatc cgatggaagt aatcgaaaag tatggagcag atgctctccg ctggttctta
     2821 tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat ggatgcttct
     2881 tggaacttta tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga
     2941 atgaccgtag aagagatcga tttatctgga gaaaaatcag tggctgaccg gtggatcctt
     3001 actcgtttga atgaaacgat cgaacgagta actgagctat ttgatcgttt tgaatttggt
     3061 gaagcaggtc gacaactgta taactttatc tgggatgatt tttgtgactg gtacatcgaa
     3121 atgagtaaag aagtacttta tggagatgat gctgtttcaa aacaaaccac ccgtagcatc
     3181 ttagtccata cacttgatca gatcctacgt ttattgcatc caatcatgcc attcgttaca
     3241 gaagaaatat gggaacatat tcctcatcag ggaaattctc tagtagtggc agaatatcct
     3301 gttgtacgtc cagagtttga tgatgaaaca gcttctaaag ggatggaagt attgaaagaa
     3361 ttgattcgag ccgttcgaaa tattcgttca gaagtgaata caccattgtc taaaccaatt
     3421 acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga aaatacaagc
     3481 tatatcgaac gcttctgtaa tcccgaagag ttgacgatat caagtgagat cactgcgcca
     3541 gatctagcga tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt agcaggattg
     3601 atcaatattg aagaagaaat caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa
     3661 gtaaaacgtg tccaaggaaa actttctaat gaacgttttg tttctaacgc gccggaagaa
     3721 gtagtagagg cagagcgtgc aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa
     3781 gaacgaatcg aaagtttgcg tactattcaa taagaataga aaaagagagt gtgaaatcag
     3841 ctagctatta ctaaaagcta gctgattgaa catcgttaca ggaaagagtt tgagcctcac
     3901 tcttagcttc gattcataag gagatgtgac ataaattttg tcgcatctct tttttagtct
     3961 actcttatta tgtaggagaa ggagggaggt ggatcatgta tcaagagtca ataaagtagt
     4021 tgataaaatc gttcctctct ctgtttcttt tctctttgtc atacttttat ttttcttatg
     4081 tgttctattt gttataataa aagagaaatg ggggaattgt tgatgacgat tgaagaagca
     4141 atagaatgga tccatagtcg attaccattt ggttcacgtc ctggccttga ccgagtcgaa
     4201 gctttattag aagtggtagg gcatccagag agaaaagtaa aaacgatcca tatcgctgga
     4261 acaaacggaa aaggttctac tgtagcttat ttgagaagcc tattagaaga ggctgggctg
     4321 acagtaggta catttacttc accttatatc gaaacgttca atgaacggat cgcaatcaat
     4381 ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa aaaaatatca gccactgatc
     4441 aaacaacttg atcaatttga agcagtagct ggtattactg aattcgagac attaacagca
     4501 atggcatttg attatttttt gatgaagaaa gtggacgtag cgattatcga agttggctta
     4561 ggcggcttat tggattctac caatgtagct gtaccaatgc tgacgggaat aacaacgatt
     4621 ggacttgatc ataccgaaat acttggagat acaattgaag aaatcgctgc acaaaaagct
     4681 ggaatcatca aacaaggcgt tccagtagta acaggaaata ttgccccaga agcttttact
     4741 gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat
     4801 aaaataaact atctgcatcc tgatgagatg tggggagaag tatttgaatt ttttaatgaa
     4861 ataggaaaga tccctcaatt aaccactccg cttttagggc aacatcaagc tgaaaatgca
     4921 ggagtagcca tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa
     4981 aaagatgttt tgaagggatt gaagaaatct cgatggccag gaagaatgga gcgtttgagt
     5041 aacgagccat tagttgtttt agatggtgcc cataatacgc atgcaatgaa ccggcttgtt
     5101 gagaatttga aaaaagaatt caaaggatat caaatcaaca ttttgttttc cgccttacga
     5161 actaaaaatg ttagagaaat gctagaacga ttgcagcaag taccaaatgt ccatatttat
     5221 ttgacgacct ttgaacatcc aaaagccatc gaattaagtg aagtatcaga tttagcaagt
     5281 gagagagtat caatcgtttc attgtggcaa ttcggattag cagatatatt ggaaaagatg
     5341 acaaatgaag acgtattagt tgtgaccggt tcactttatt tcatttcaga agttcgccat
     5401 ttattaatac aattaggagg agatcatgaa gtataatgca gtgatttttg acatggatgg
     5461 tttgctgttc gacacagagt tagtgtatta cgaagccaca caagtagtag cagacaagat
     5521 gggttttcct tatgataaag acctgtattt aaaattctta ggtgtttcag atgaagaagt
     5581 atgggcgaat tatcatatga ttttctcgga atttggcaaa acaagtgtac aaagattcat
     5641 agatgattca taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc
     5701 cggtgtgttg gaactcctct cttttttaga agaacatcag attcccaaag ttgtcgcttc
     5761 cagtaaccaa cgagcagtca ttgaactttt attgaagaag aacaatctgt ttgaacgatt
     5821 tgagacgatt gtttctgcag aaaatgttca acgagcaaaa ccagacctag aaattttctt
     5881 gttagctaat cagaagctag gtacagcgaa gaaagaaaca ttgattttag aagattcaca
     5941 aaatggtatt ttagcagctt ctgcggctga gatcccggta gtcatggtac cagatctgct
     6001 tccaccatcc caagaattga tcaaaaaaac agctggggtt gtttcttctt tacatgaaat
     6061 ccctgcattg ttaaaataaa aatccatctc ttttcgtatc ttattttaga taagatagaa
     6121 aggagatgtt tttttgggaa aacgattgat caaagaagta cctactagct ctttaccaag
     6181 ggaacgtatg gaaatttatg gcgcggaagc tttatcggat caggagcttt tggcaatctt
     6241 actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac
     6301 gtttggagga ctggcctctt tacgacaggc tactttgcat gagttagaag agattcgagg
     6361 gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc gacgaattca
     6421 gacggatcat gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg cccagcaatt
     6481 gattctggaa atgaaagatc acaaacaaga acatttggtt tgtatttatt tggacacgaa
     6541 gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt cagtcgctca
     6601 tcctagagaa attttccatt atgctgtacg ttattgtgca gcaaggatcg ttttagcgca
     6661 taatcatcct tctggtaatg ttttaaccat agtataataa ataatagtag ttaaagtagt
     6721 ttaagaggag atattaaagg gatattttac tttaaataaa aattaacaag gtttataatt
     6781 tgtagtagtt aaagtattta aagaggtggg aacatgtgaa ggttcaaagg atagaagtgg
     6841 agaataagcc gtatccattg tatttattac tagataaaga ataccagcta atagaaccag
     6901 taatgaaatt tattaaatac ttagataata ctggtaagtc tcctaatacc attaaagcat
     6961 actgctatca tttaaagttg ctgtacgagt tcatggaaca gagaggtgtt attcttaatg
     7021 atattaactt tgagttgtta gcagacttcg taggttggtt gagatatcct tcagcatcaa
     7081 atgtaattga tcttcagtca aaaaaagcca taagagaaga aacgacagtg aatacaattt
     7141 taaatgtagt tatgagtttt cttgattatt taagtagatt aggagaattt aaatcaattg
     7201 atgtatttaa acaagcaaag ggaagaaatt tcaaaggatt tttacatcat gttaataagg
     7261 gtagatacca aaagaatgtc ttaaagttaa gggttaaaaa gaaacagata agaacattga
     7321 gatcagagga agttaagcaa attattgatg cttgtcatac gaaaagagat aaattaattt
     7381 taatgcttat gtatgagggt ggtttaagaa tcggtgaagt gttatcgctt aggcttgaag
     7441 atattgtcac ttgggacaat caaatccatt taacacctag agatgttaat gttaatgaag
     7501 cttatattaa attaaggaag gaaagaacaa tacatgtgag taaagaactt atgtcacttt
     7561 atacagatta cttgatatat gagtatagtg aggaattgga gcatgattat gtttttattt
     7621 ccttaaaaga aggctatttt gggaaaccac taaagtacca aagtgttctt gatctagtta
     7681 gaagaatagt taaaaggact ggaatagaat ttacatcaca tatgcttcgc cacactcacg
     7741 caacgcagct aattagggaa ggatgggatg ttgcgttcgt tcaaaagaga ttaggtcacg
     7801 cacatgttca gacaacgtta aatacctatg ttcatctttc agatcaggat atgaaaaatg
     7861 agtttaataa atacctcgag agaaaggagc ataagaaatg aatgcttcta gtaaaaggaa
     7921 aattattagt cagagtgaga ttagcaaaaa aatagctgta atgaatgaag aaatgcaggg
     7981 gttttgggct aataatagtt gggatataag aaaatgtcca catccttctg ccatagaatt
     8041 aagtaagaat cctgctttaa ggaatcgttg ggttcgtttt gaacgtgtta aaaatctgtg
     8101 gttaagaaca gaattgaaat atttttattt ttaccattta aacaatggaa tatggaatgc
     8161 aaaaactgtc tggattagaa aaggaacagt aattaataaa atgttagatt tcttagattt
     8221 aaagtatcct agcattactt caattactga agttcctatt gaaaaagcaa tgacggagta
     8281 tagaacttat ttgacaaaac ggggtgttag aattaccact actaattata agattactgc
     8341 taatcaagaa aaaacacctg taaaagctaa ttcctactat gttactaatc taaaacaatt
     8401 tatggagttt tatgagaact tttattttga tggagaggag tgggataaag acgtttggga
     8461 tagacgtaac ttacctttgc cagatgataa ggttaaccca acacaatatg aatatacaat
     8521 taactttaaa gggtttcgga atacatattt taaacaactt gtaaaaagat attgtaagtt
     8581 gagattgaac atggatagct tttcctatgt aagtgatatt gcccaaagac ttaaagagtt
     8641 ctttaatttt ctggacatga aatttaaaca agttcagaga gtacaccaat taacgagagt
     8701 ggaaattgaa gcatatttaa gtgaactaaa catgatggga ataaaaccta gtacaataac
     8761 tgggaggatc tctatattgg aaggactatt tagtaccctt cttaggctag aatgggatga
     8821 tgttccttcc aaaatattaa tttattctga ggactatccg aaaataccaa gagcaaaacc
     8881 acgctttata gatgaattcg tcctagagca attgaacagt catcttgata aattacccga
     8941 atatatagct acgatgacta tgattgttca agaatgtgga atgaggataa gtgaattgtg
     9001 caccttgaaa aaaggctgtc tattagagga caaagatgga gatttctttt taaagtatta
     9061 tcaatggaaa atgaaaaagg agcatatagt tccaatatct aaagaggtag ctttacttat
     9121 taaagttcgg gaagataaag tttcagagga atttccagat agtgaatacc tctttccaag
     9181 aaaagatgga tcgccattaa aacaagaaac atttagaggt gagttaaata aattagctta
     9241 tgagcaaaat atagtggata aatcaggtga gatttataga ttccatgccc atgcctttcg
     9301 ccatacagta ggaacaagaa tgattaacaa cgggatgccc cagcatattg tgcagaaatt
     9361 tttggggcat gaaagcccag aaatgacaag cagatacgct catatctttg atgaaactct
     9421 aaaaaatgaa tttactaaat ttcaggaaaa actggttacc aataatggag atgtgcttga
     9481 tctagatgaa gataatgaag tcgatgatgt agagcttcaa tggttcaaga aaaatataaa
     9541 tgcacaagtg cttccaaatg gttattgtag attgccagta gtagcaggtg gttgtccaca
     9601 tgcgaatgca tgcttagatt gcactcactt ctgtaccagt aagcaattct taccacagca
     9661 cgaagaacag ttagagcgta cagaagagtt attagccata gctaaggata aacaatggca
     9721 aagacaagta gagactaata gccgtgttaa agagcgttta gaacaaatca ttggaagttt
     9781 gacggggtaa ttatcaatgg ataaacaagt tagaaataca acagaaattg tacgtttggc
     9841 gaagcagaaa tcaaaaaaga caagggaaaa agtagacaaa gcgatttcta aattttcgat
     9901 tgaaggtaaa gttattaatt ttaattcaat agcaaaggaa gctaatgttt ctaaatcatg
     9961 gctttataag gaacacgata ttaggcaaag aatcgaatcc cttcgtgagc gtcaaataac
    10021 agcaaatgta gtctcaaaac ccaagaaaag ttctcgttcg gaggaaatcc ttattaaaac
    10081 cttaaaaaga agagtaatgg aattagaaaa agaaaataaa aaattacaga accaaattca
    10141 aaaattatat ggagatctgt ataataaaga ataattatta atctgtagac aaattgtgaa
    10201 aggatgtact taaacgctaa cggtcagctt tattgaacag tcatttaggt atatgtccaa
    10261 tctagggtaa gtaaattgag tatcaatata aactttatat gaacataatc agttacgagg
    10321 tgaaatcatg agcaatttga ttaacggaaa aataccaaat caagcgattc aaacattaaa
    10381 aatcgtaaaa gatttatttg gaagttcaat agttggagta tatctatttg gttcagcagt
    10441 aaatggtggt ttacgcatta acagcgatgt agatgttcta gtcgtcgtga atcatagttt
    10501 acctcaatta actcgaaaaa aactaacaga aagactaatg actatatcag gaaagattgg
    10561 aaatacggat tctgttagac cacttgaagt tacggttata aataggagtg aagttgtccc
    10621 ttggcaatat cctccaaaaa gagaatttat atacggtgag tggctcaggg gtgaatttga
    10681 gaatggacaa attcaggaac caagctatga tcctgatttg gctattgttt tagcacaagc
    10741 aagaaagaat agtatttctc tatttggtcc tgattcttca agtatacttg tctccgtacc
    10801 tttgacagat attcgaagag caattaagga ttctttgcca gaactaattg aggggataaa
    10861 aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg tggcaaacag tgactactgg
    10921 tgaaattacc tcgaaagatg tcgctgcaga atgggctata cctcttttac ctaaagagca
    10981 tgtaacttta ctggatatag ctagaaaagg ctatcgggga gagtgtgatg ataagtggga
    11041 aggactatat tcaaaggtga aagcactcgt taagtatatg aaaaattcta tagaaacttc
    11101 tctcaattag gctaatttta ttgcaataac aggtgcttac ttttaaaact actgatttat
    11161 tgataaatat tgaacaattt ttgggaagaa taaagcgtcc tcttgtgaaa ttagagaacg
    11221 ctttattact ttaatttagt gaaacaattt gtaactattg aaaatagaaa gaaattgttc
    11281 cttcgatagt ttattaatat tagtgacatt tgcatgcttc aaagcctgtc ggaattggtt
    11341 tttagtgaaa agaacacgat attcacggtt tacccactta taaacaaaag atcgatactt
    11401 tttgtagtcc ttctttgaaa tcaatggttg atgtcgttca agaacaatca atacagagtc
    11461 tacacttggc ttaggatgaa aatatagtgg tggtactttt ttgagcattt ttatatccat
    11521 ctccaccatt aatagtaaac ccaaagctcg ttgcagattt tgcaatcttt tcgcaaatcc
    11581 cttctcaacg ataagatagc tatatttagc ctgactttca aaggtaattc ttttgacaat
    11641 atccgtactg atgttataag gaatattacc atatatctta tagtttatat gttttgggaa
    11701 ggaaaatttt agaatatccg tttgaatcac ttttatattc tcagaggggt ttaccgcttc
    11761 tttagtcact tgacataagc ctccatcaat ttctatagca gtaactgatc gactcatttt
    11821 gactagctct ttggtaaaat gtccttttcc tgatccgatt tctattacgt tgtcttgttt
    11881 actgatattc gtgtgattca atatttcttt tacatgcttt ttagaagtaa taaaattttg
    11941 cgtgtcttta gggtttttct ggttcattat aaccttctcc ttactggtta taatgaactg
    12001 acattaagcg ttcattataa ccaattaatt ttgatttggt tgataacgaa ctttattaat
    12061 aacaaatata gaaaaagtac ccatactttt atctcctttc catttttttc attatgaatg
    12121 agataaagta atttctacta ctgcgatact ggtgcacata attgaaacct ccttatgtaa
    12181 ctgaatatga ttgtatcatc ttttcatttt agaataaacc tttatatgta attttgcact
    12241 tgacacttca gatggacgtt ttcttcaaaa taatatgttt tgaaacttga taagagaagc
    12301 cactgtcttt actaaaaagt aaaggctgcg ataaaggaca gtgactttaa taaagttgca
    12361 ctgttgtatt gtctttggat aaaattgtct aaagggaagt tttatcccca acgaaaaaac
    12421 atctaaagtg gtaagtaatg aaagaatgtc gataaagtag gaatggggga tctttttgaa
    12481 tacaatagaa tcacagtttg ataaggttgc agaagattac gatttcgtga atgagctttt
    12541 gaatgattat tcattctttg tgtctaatat gtctccaaag aaaggcagag cattagatat
    12601 cggatgtggc tcgggtttgt tagtggagaa attagcaagt tattatgatg aagtggtagg
    12661 gattgatatt tctaatcaaa tgctcgatct tgccaaatct aaacgtcaac taacaaatac
    12721 ggtctatctg aatatgaatg cagaacaact taattttaat gagaagtttg attttattgt
    12781 aagccgaaca acctttcatc atttggatga tatagccagc gtaatacagc aaatgaagga
    12841 actgttgaat gaggaaggaa gaatagttat tcttgataat gtatctgaag tcgagacacc
    12901 gcctacctat gtttataaat taggggcaat ccaagagttt ttaccgcatt gtttcaaatt
    12961 tggaataaaa aatgcgatta gaatttataa tcacaacaca tctaaatcat ggcttgagca
    13021 tttagcttca gataagtacc tatctgaaca aaattattac gatttatatg aaaagttgtt
    13081 gcctggatgc caattccata aaatgggttg ggcaatgggg gttgtctgga caaaataact
    13141 taaagctgtg gctttgaaaa gttgaatttg tgattgtttt cagagctatc gggtgcttta
    13201 gtataaataa caatgaataa aaaatcagcc tgtatcttat gttactatta agtaatttaa
    13261 gatacaggtt ttactatttt tgaaataagt accttccact tcaaatacat gtattcaaag
    13321 tgaaagtatt gatttaaatg attcttttgt aatttactct tgataatatg aaattccatc
    13381 gcagcaggat atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac
    13441 tgtactggac cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat
    13501 gatggaagag aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt
    13561 gtaattttgt ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa
    13621 tgttacaatt taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca
    13681 gtaaaatggt tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac
    13741 gtattcgttc acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa
    13801 gcagtgactt tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa
    13861 gcttaatttt atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag
    13921 cgagtaacaa aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta
    13981 tgaaaaagta aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa
    14041 caaacaaaaa tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc
    14101 tgatttcata acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg
    14161 gatgtctttc tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca
    14221 atttatattc tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc
    14281 gctgagcttc attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa
    14341 tatctggacc agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa
    14401 gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc
    14461 cgctaccatc gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc
    14521 tagttaacgc taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta
    14581 catcttgtgc tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa
    14641 aatattttgg cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg
    14701 aagtatggat gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat
    14761 tgatcaaatg attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg
    14821 tatcgtctgg ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg
    14881 ccatcgcttc tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat
    14941 tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac
    15001 cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca
    15061 cagcggcatc tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga
    15121 tacgtccttc gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt
    15181 tagtaaatgt tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt
    15241 ccccgatacg agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt
    15301 tgaagtctcc agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat
    15361 ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg
    15421 tatcttcatt gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt
    15481 ttgtgatcaa cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca
    15541 agtagggtat tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt
    15601 tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat
    15661 cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc
    15721 catacgtatt tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat
    15781 ttttgatgga aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg
    15841 tactctagat gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct
    15901 tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct
    15961 tcccgtttag ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg
    16021 tctcctttat ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac
    16081 gaggggacaa gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa
    16141 gagacaagcc tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata
    16201 aaaaatatac aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca
    16261 actagattga aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta
    16321 tgagagaagt gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa
    16381 caaaagatta aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa
    16441 gaaattcttg tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact
    16501 tcctacgatt ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt
    16561 gaagcaacaa gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt
    16621 gttaggttat tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt
    16681 gttcgaaaag atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt
    16741 gatcactcgt acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg
    16801 tatcggtttt atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag
    16861 tgtgacactc agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc
    16921 gattgctaag gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa
    16981 cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa
    17041 gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt
    17101 attccgtttg atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt
    17161 attgatcatc tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt
    17221 gattgcgttt atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt
    17281 gttcatgatg tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc
    17341 caaaccttcg attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat
    17401 cgaatttcaa aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa
    17461 tgtcagtttt acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg
    17521 aaaatctacg ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt
    17581 gatcgatgga gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt
    17641 tattccgcaa aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa
    17701 agaagatgct acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt
    17761 catttctcga aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc
    17821 aggtggacaa aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta
    17881 tatttttgat gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg
    17941 tctgaaaaaa gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat
    18001 catgcatgca gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca
    18061 tcgggagttg ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa
    18121 ggaggaatta gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga
    18181 ccgtatcgtg agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac
    18241 gtggctttgc catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat
    18301 ggcgaatcaa tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga
    18361 atagggtatt gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt
    18421 gccatgcatg atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat
    18481 ttcgataaaa accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc
    18541 agcggtgctt tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg
    18601 gctgcagcaa tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt
    18661 ccaacatcga tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc
    18721 atgcagaatt ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt
    18781 gtattgaaag tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac
    18841 agcttgaaat actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt
    18901 caattaactg cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt
    18961 actggggcga tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt
    19021 cagccaatgg ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg
    19081 aaacgtgtat tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca
    19141 ctgccagaaa cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg
    19201 aagaaaccat taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg
    19261 gtcggtccga ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac
    19321 gtagacaaag gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat
    19381 gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca
    19441 gataatatcc gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa
    19501 acagcaaacg tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat
    19561 tctgagggag acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg
    19621 gtctccgatc ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta
    19681 gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc
    19741 gctcaccgtt tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag
    19801 atcattgaaa aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg
    19861 tataacagcc aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa
    19921 cttatttttt gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga
    19981 cacggagatg gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg
    20041 tttaacgcac gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt
    20101 tcaaatcgaa cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat
    20161 agaagaaaat atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa
    20221 tagacatctg gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta
    20281 tgataccttt attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg
    20341 gaacggttta tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg
    20401 acgatctggt atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga
    20461 acgaatatcc aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg
    20521 gagaatgtga cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg
    20581 ctgatcggga tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt
    20641 ggtgctggat cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt
    20701 cgcccagcta aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac
    20761 atccagcatt atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga
    20821 taggcagtag catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt
    20881 atttggttta gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag
    20941 aactgttttc catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat
    21001 agttgtcacg atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta
    21061 aaattttgtt taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag
    21121 ttgcttgccc ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag
    21181 caaatcgttt accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac
    21241 ccataacaat tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac
    21301 ctgctgtaac tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt
    21361 gagcgtctaa gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc
    21421 cgcttaacat gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac
    21481 ctcgtgaaga ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt
    21541 tcgtttagaa atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt
    21601 tggtcaaaaa aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg
    21661 tatgataagc agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc
    21721 cttatcggag tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt
    21781 cacttggaaa ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag
    21841 acgctgaatc aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg
    21901 gaagaatatg tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg
    21961 tatctccaat cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag
    22021 ctatttaaga atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt
    22081 ggtggatatg cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact
    22141 gaaatgatca atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc
    22201 ttctcgaaag atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg
    22261 tggtaatgct ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg
    22321 ttacaaatct atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca
    22381 tttgcagaag ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa
    22441 gatggttatt tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg
    22501 ctgattcgag aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc
    22561 aaagaatttc cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa
    22621 aaaaattacc gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt
    22681 atcgaagaaa aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat
    22741 tgcagctttc atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg
    22801 gacttattga tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata
    22861 gaaggagtac atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt
    22921 ccaaaagcaa ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa
    22981 ctcaaaatag atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca
    23041 acgaaaagta ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt
    23101 aaaacagcgc taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat
    23161 tcattgcaaa tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg
    23221 aataatgttc cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct
    23281 gattttcagc attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct
    23341 atcatagctg gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga
    23401 gcgacagaag aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt
    23461 ggttcccaag cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc
    23521 cactggaaca atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat
    23581 agtatcacgg cacctaaaga agagccaaag caattgtatg attacctcat gattggatta
    23641 gatacgaagc gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca
    23701 gaaggtttag tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa
    23761 ggtattggat ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca
    23821 acagaacaag tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt
    23881 aaccgtttgg atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt
    23941 gagcaaacaa tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt
    24001 aggggtagaa accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca
    24061 gctgacgaat accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt
    24121 ggatcggcaa acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc
    24181 gcatgaagcg gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat
    24241 taccgatgct gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc
    24301 gatgcgtgct cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct
    24361 gctgcctcgt ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac
    24421 aagagggcca ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt
    24481 tgctgttaag aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag
    24541 aaaaaatagc gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac
    24601 tatcttgaat ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt
    24661 agatccattg actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac
    24721 cgtcgggttc atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga
    24781 agaaagtcaa aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat
    24841 tttacaggag cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat
    24901 actgactgtt tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt
    24961 cccaaatgta ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat
    25021 caaacgtcag ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg
    25081 acagaaatta agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga
    25141 aacaaacgaa tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa
    25201 ccaataaaat tttatttttt taa
//
LOCUS       DAPCMU010000024        24982 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPCMU010000024 DAPCMU010000000
VERSION     DAPCMU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.024"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            178..747
                     /locus_tag="RD744_000839"
     CDS             178..747
                     /locus_tag="RD744_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0556865.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(925..1365)
                     /locus_tag="RD744_000840"
     CDS             complement(925..1365)
                     /locus_tag="RD744_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDU0556866.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1523..2287
                     /gene="map"
                     /locus_tag="RD744_000841"
     CDS             1523..2287
                     /gene="map"
                     /locus_tag="RD744_000841"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDU0556867.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2319..3242
                     /locus_tag="RD744_000842"
     CDS             2319..3242
                     /locus_tag="RD744_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDU0556868.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3318..4457
                     /locus_tag="RD744_000843"
     CDS             3318..4457
                     /locus_tag="RD744_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDU0556869.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4450..5250
                     /locus_tag="RD744_000844"
     CDS             4450..5250
                     /locus_tag="RD744_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0556870.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5250..6077
                     /locus_tag="RD744_000845"
     CDS             5250..6077
                     /locus_tag="RD744_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0556871.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            6055..6789
                     /locus_tag="RD744_000846"
     CDS             6055..6789
                     /locus_tag="RD744_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0556872.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6889..7755
                     /gene="rfbA"
                     /locus_tag="RD744_000847"
     CDS             6889..7755
                     /gene="rfbA"
                     /locus_tag="RD744_000847"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDU0556873.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7769..8341
                     /gene="rfbC"
                     /locus_tag="RD744_000848"
     CDS             7769..8341
                     /gene="rfbC"
                     /locus_tag="RD744_000848"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDU0556874.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8363..9391
                     /gene="rfbB"
                     /locus_tag="RD744_000849"
     CDS             8363..9391
                     /gene="rfbB"
                     /locus_tag="RD744_000849"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDU0556875.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9489..10340
                     /gene="rfbD"
                     /locus_tag="RD744_000850"
     CDS             9489..10340
                     /gene="rfbD"
                     /locus_tag="RD744_000850"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDU0556876.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10375..12408
                     /locus_tag="RD744_000851"
     CDS             10375..12408
                     /locus_tag="RD744_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556877.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12452..13732
                     /locus_tag="RD744_000852"
     CDS             12452..13732
                     /locus_tag="RD744_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDU0556878.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13942..14748
                     /locus_tag="RD744_000853"
     CDS             13942..14748
                     /locus_tag="RD744_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556879.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14760..15980
                     /locus_tag="RD744_000854"
     CDS             14760..15980
                     /locus_tag="RD744_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556880.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15970..17556
                     /locus_tag="RD744_000855"
     CDS             15970..17556
                     /locus_tag="RD744_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0556881.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17595..19733
                     /locus_tag="RD744_000856"
     CDS             17595..19733
                     /locus_tag="RD744_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0556882.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(20101..21084)
                     /locus_tag="RD744_000857"
     CDS             complement(20101..21084)
                     /locus_tag="RD744_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDU0556883.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21462..22853
                     /locus_tag="RD744_000858"
     CDS             21462..22853
                     /locus_tag="RD744_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDU0556884.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22869..23909
                     /locus_tag="RD744_000859"
     CDS             22869..23909
                     /locus_tag="RD744_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0556885.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23928..>24982
                     /locus_tag="RD744_000860"
     CDS             23928..>24982
                     /locus_tag="RD744_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDU0556886.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         8055 a         4114 c         5108 g         7705 t
ORIGIN      
        1 ctttaaataa aaaaaggagg ttattttttt aaggttaagt ttcatgatat atgttactac
       61 ttgctatttc agcttgtgaa gaaaatcaaa aactaatttt gcatatcaaa aaaacatgtt
      121 acaatgaaat taatcaatgg tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg
      181 gcaagaaaaa agacgattac tagagaacag attttggcag ctgcttatga agtagctgcc
      241 accgatggat ttacacgatt tactgcgaga aacatcgcga acaaaatgaa ttgttccacg
      301 caaccgattt atcttgagtt taaaaatatg gatgacttga gacaagctct ttttgagcag
      361 atctatgatc atttacaata tgaagtgttc actgttgagc atacagggaa tacgattatc
      421 gatcttgctt ttaattatat ccattttgca agaagagaga agaagttgta tcgttcactg
      481 tacttagaag aatatggtgg aggaaaagag atgcagaatt tctcttatac ttactttgtg
      541 aatacagtga aacgcgatcc agcttatgtg aatctttcag aagagcagat cgaatctttg
      601 tataatggga catggatcat agctactggg atcgcagctt tgatgtcttc aagcatcatc
      661 catccaactg atgaacagat tgaaaagatg atccaagaca gtatagatgc tatcttgaaa
      721 ttcaaagaac caatcgaaat cgattgaata tactcaaaag ttccacaaat cagtaggtaa
      781 caagagatgt gtcaaaataa ggaaaagttc gtttttctta cagcgtatgc atttttaact
      841 cccgaagaac tcttccatga atgcaatgaa taaaagagcc atacagattg aataaatctg
      901 gatggctctt ttattgcaag tatattaaat ttttgcgact agttctttag cgaaagcttc
      961 taaattttcg atatcttctt cttcagcatt cagatccact ttaacatttt cggctccttt
     1021 tgatgcgcct gtttttgcaa aagcagcatc gaaatcatcc accgatttgc agaattcatc
     1081 gtaaaaagta tcaccagatc cgcagacacc gtaaattttt cccaatagat ccaattcttg
     1141 caagtcttca taaaagtcta cgatctcatc aggcagatcg ccatcatcgt atgtataagt
     1201 agctactacg caaatatctg cctcctcgaa ttcatctgca tctacttggg tacattcgtt
     1261 gatttcgact tcgatatcca gattttcaaa tgcttctgca acaatatcgg cgatttcttc
     1321 agtgtttcct gtcatgctag catatacgat ttttgctaaa gccatgatgt gcctcctcgt
     1381 ttataattat ttaacaatta tgaattatac caaaacatca ttgaaaagaa aataaaacct
     1441 aaagacacct ggggaatgtc atttggctaa atgtatgccg ctatgttaaa atgagaggga
     1501 tttgtaaaaa ggagacgaaa atatgattac tttaaaatca gcacgtgaaa ttgaaatgat
     1561 ggcagaatct ggagctttat tagcagatgt acataaaaat ctgcgtgatt ttataaaacc
     1621 aggaatcaca agttgggata ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg
     1681 aattgctgcg caaattggct ttgaagggta tgaatatgcg acttgtatca gtataaatga
     1741 tgaaatttgt catggattcc ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt
     1801 cgatatgtgt atcgatttga aaggcggcat ttctgattct tgttgggcct atgtagttgg
     1861 agaatctaca cctgagatcg accgattgat ggaagtaacg aagaaagctt tgtacatcgg
     1921 tattgagcaa gcacaagtag ggaatcggat tggtgacatc ggtcatgcta tccaaactta
     1981 tgtagaaggc gaaaatctag cgatcgttcg tgattttatt ggtcatggcg ttggtcctac
     2041 gattcatgaa gaaccagcag ttcctcatta cggagaagct ggaaaagggt tgcggttgaa
     2101 agaaggcatg gttatcacga tcgaacctat ggttaatacc ggaacttgga aaatgaaaat
     2161 ggatcctaac ggctggactg cgtatacaag agacggcgga ctttcttgcc aatatgaaca
     2221 tacattggct attactaaag atggtccaag aattttgaca tcacaaggag aagaaggcac
     2281 ttattgatct atccagtcaa taggtacggg agaggtacat gaaagtttgg aacaaaataa
     2341 aacaaaatga aaatctgatg cgctttattg agacgactca aaagcgcatc actgattcag
     2401 aaatgagtac gacctctgtc gtcgttgctt attatttact cttgtcttta tttccattga
     2461 ttattgcttt tggtaatatt ttaccgtttc tccacatcga tcaagagact gtactgacct
     2521 atttacgaga agtgattcct gagaccgttt accagtttat cggtcctgca atcaaagacc
     2581 ttttgacaca aagttcagga ggcttgttat ccgtttctgc attagcggct ttatggtctg
     2641 caagtcaaag tatcaatgct ttgcaaattg cgatgaataa agcgtatggc gtagaaaata
     2701 gaaaaaattt tttgatcgtc cgtttcttct cactaattgt cattgtcttg ttcatgattg
     2761 ctattagtgg ggtaactatc gttcttgggt tgggacagct gattttggat gctattcagc
     2821 caatcgtaca aatacccgtg aactttatcg atcagttcca aactttgaag tggccaatca
     2881 cattggtagc tttatttgtc atcatgttct tgatttattt aattgtccct aatgctcagt
     2941 tgaagttgaa gacgatcatt ccgggcacga tcttcgctac tgtcggctgg atgctgttat
     3001 cacaagtgtt tagtatctat gctaggtatt ttgcaacgag gatcagcggt tatcagatca
     3061 taggaagttt tattgtactc atgctttggc ttaattttgc tgctacgatc atcattttgg
     3121 gcggaatcat caatgcagtt gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc
     3181 gagatccaag ccgaaaatgg ttcaataaat tgaaaactaa attttctcat aaaaaaaagt
     3241 agaaacaaga gttcttattg taaatttata tcagtaattt ataataatta tgactcttaa
     3301 ttaaagtagg tgcccatatg cttgtttctt ttagtatggt tataagattt tttctgacaa
     3361 ttttattatc actgatatta acacctgtat tcaaaatcat ttcagtacaa acaggtatgg
     3421 tcgacaaacc aaatgaaaga agaataaata aagttccaat gccttcagcg ggcggactgc
     3481 ctatctttat ttcatttgtt atatcgactt tattcttatt ccgtaaaatt atccctcaat
     3541 cgtatattgt accgatcttg attgcttctt tagtaatcat agtcacagga gtgctcgatg
     3601 ataaatatga gctgacacct aaacaaaaaa gtatagggat cttgattgct tctcttatta
     3661 tttattttgt agcagatata cgtattgatt ccttcacgct tccttttatt ggatatatcc
     3721 aactaggctg gcttagtttt cccgtaacga ttttttggat cttcggtatc acgaatgcgg
     3781 tcaatctgat tgacggtttg gatgggctgg cggcagggat ttcattgatc ggtttaatta
     3841 cgataggaat cattggatat tttttcctcc atgcttctac agtgtatatt ccagttgtta
     3901 ttttctgtct tgtagcaagt attatcggtt tttttcctta caatttttat ccggcaaaga
     3961 tttatttagg cgatacagga gctctattcc tagggtttat gatggccgtc ctttcactac
     4021 aagggctgaa gaacgtgact tttgtttcat cgatttcttt gctgatcgtc atgggagtgc
     4081 cggtaacaga cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt tctttttcta
     4141 cagcagataa aaaacatctt catcaccgac taatcagttt aggctttact cataaaggtg
     4201 ctgtcctgac gatttatgcg atggctttga tgttttcttt cacagctatg gtcatgaatt
     4261 atactggaag aatcggaacg atcgtattga ttattgccat gttgtttgct gcaattttat
     4321 tatttgaatt gattggtctg atcaatgaga aacatcagtt tattttaaaa acattgcgat
     4381 ttttagggaa taaagagtac cgtacgcaag taaaacagaa atatttgaag aaatggaacc
     4441 gtcatataga tgaataacaa attaaaccag ccgatcctta ttagtattgt gacggcaaac
     4501 agtgataaaa tttttcggac actagataat tttatttcaa ctgtaaaaga tgaaacagca
     4561 gtaaaaatcc gaatttttga caacaattca cttcccgagt acaaaaaaag actaacagag
     4621 tataggaaat atccctacat cgatatctat tttcatgatg aaaattccgg atttggttat
     4681 ggacataatc acaatctgat agctagcgat tctcgctatg gcattatttg taacccagat
     4741 attttagtgg atgaagagac agttacagca ttggtcttgt tattgaaaga acatccggaa
     4801 tgtgcgatgt tagcacctaa gattcttaat gaagatggaa caactcagca cctcatccgt
     4861 gagcagctgg ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa
     4921 aagtttgaca aacgactggc tgtgtttgaa tgccgaggat tgcctactga tgctaatagt
     4981 tatgtacgga tgatctcagg tagttttatg gtagctgata ttgataaatt taaagagata
     5041 aaggggtttg atgagcgttt cttcatgtac tttgaagaca acgacttgtg tttgagtttt
     5101 gaaaaagcag gttacaagtt attgtacaca cccttttatg cagttatcca tttttacggg
     5161 aagggtgcgc atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat gatgaaattt
     5221 ttcaataagt gggggtggcg ttttttctga tgaaaccaga aatattagta gtcgttgtgc
     5281 tttatcagca actattcagt caatctccag cttatggacc attaagcaaa gcacttagcg
     5341 aaaagcaaat tcagcttatt atttacgata atagtcctca aaggcagcaa aatgagctgt
     5401 ttgaaaaaga aaatatcgtt tatcatcatg atccgcaaaa tcctggttta gcggtggctt
     5461 ataattttgc gttgtctcaa gcagatgaag gaacacgttg tctcgtcact ctggatcagg
     5521 atacgaggct agtcgacgat tattttgaac tattgcgtaa agtaaccttt acagatgaat
     5581 gtgttgctgc agttccgatg attttttctg ggagccgaca agtttctccc ctatattcag
     5641 atcgttatat caatcgtaca gctcaagcag tagaagttga aggcccaaca gctgaacgaa
     5701 tcatggcaat caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt
     5761 ttaatcctgc tttttcttta gattttttgg atcattggct gttttggaaa gtggatcagt
     5821 tggaaaaaac tgtggagatc cttcctgtac gaatggaaca tgatctttcg gttttagact
     5881 atgaaaaagt ttcaagtaat cgataccgtt ctattttaca agcagaaaat cgattttatc
     5941 atgaatatga tcaaaggcat ttatccagtc accgacgaca attattgtta agaacagcta
     6001 aacagttctt gaccgtcaaa aaccggcata tctggcggat gactttgcgc tcatatgtta
     6061 agaattggaa ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga
     6121 tatttaagag agcagttaga tagtatttta tctcagcttt cagcagaaga tgaagtagtc
     6181 atttctgatg atgggtcagt agatgataca tgggatatat taactgaata cgcagaaaaa
     6241 gataagcgaa tccagctttt ttcggggcct cgtcaaggat tgatcgctaa ttttggttat
     6301 gcgatccagc agacgaaagg agatctaatc tttttggcag accaagatga tgtttggttg
     6361 gagacaaaag tgaaaaagat cactgactat tttgagcaga atccggatca gctagtagta
     6421 gtttctgatt taatcattgt ggacagcgag ctgcaagaga tccatgattc gtatttttcg
     6481 tttcgaaatt ccaaagaagg atggcttaat aatgtgataa gaagtcacta tattggtgct
     6541 ggtatggctt tccggtcttc tttaaaaccc gatattttgc cgatcccgtc agaagtgccg
     6601 atgcatgata tgtggattgg tttgctggga ggaaagaaga ctgggttcct aagagaaccg
     6661 ttaactctgt accgaagaca tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta
     6721 cggcaaaaaa tttcttggcg tgttcatctg attacggcgt tgatcaaacg aaaatggttt
     6781 aaacgttgat aaaagatgtt taaatcctta atatttccat gacaaatata acaattgatg
     6841 agataataac aaagtgaaat ttgaaatcaa aaaggagagc agatatccat gaagggaatc
     6901 attcttgctg ggggcagcgg aacacgtctg taccctttaa caaaggcaac atcgaaacaa
     6961 ttgatgccga tttatgacaa accaatgatt tattatccaa tgtctactct gatgttggcg
     7021 ggaatcaaag aaattctgat tatttcaacg ccacaagata caccacgttt caaggaatta
     7081 tttggaaacg gaaatgattt aggactgcag atcgaatatg cagtacaaga aagtccagat
     7141 ggattggcac aagcgtttat cattggagaa gaatttattg gagatgatag tgtctgcttg
     7201 gtattagggg acaatattta ctatggtggc ggtctgtcta aaatgttgca acgagcagct
     7261 tcaaaagata caggcgcgac agtatttggc tatcatgtca atgatccaga acgctttggt
     7321 gtagtagaat ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca
     7381 aaatcaaatt acgctgtaac tggcctttat ttttatgaca atgaggtaat ttctatcgct
     7441 aaaggtatca aaccttcaga acgtggagaa ctagagatta cggatgtcaa taaagcatat
     7501 ttggataaag gaaaactatc cgtagaactt atgggacgcg gatttgcttg gcttgataca
     7561 ggaacacatg aatcattgct tgaagcatct acctttatcg aaacaattga aaaacgtcag
     7621 aacttaaaag tggcttgttt ggaagaaatt gcttaccgta tgggctacat cgataaggaa
     7681 caattgctgg cccttgcaca gccattgaaa aaaaatcaat acggtcaata tttattgaat
     7741 ttggccgctg aataaggagt ccttacagat gaaagtaaca gatacaaaat taacagatgt
     7801 caaaatcgta gaaatggacg tctttggcga tcatcgcggt ttcttcacag aaagttattc
     7861 aaaagcaaaa tttgcagaac atggattgaa atatgacttt gttcaagaca accattcact
     7921 atcagctgaa gctggtgtcc ttcgggggct tcatttccaa aaaggagaag ctgcacagac
     7981 aaaattgatt cgggtagtca ctggagcagt tttagatgta gtagtggata tccgcaaagg
     8041 tagcccgact tatggtcaat gggacggatt tatcttatct gaacataatc atcgacagtt
     8101 gcttgtacct aaaggatatg cccatggatt cgttactttg acaccaaatg tgaacttcct
     8161 ttataagtgt gataattact ataatgcaga agcagatggg ggcatcgcat ttgatgatcc
     8221 agatttagcg attgattggc caattgatcc atcaagagcg attatgtcgg aaaaagatca
     8281 ccgtcatccg acattaaaag aatttgaagc agaaaatcca tttgtttacg gagaaatcta
     8341 agaattaagg agaaattttc ttatgaaaaa catcattgtt accggaggtg ccggattcat
     8401 cggctctaat ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg tcactgtttt
     8461 ggataaactg acttatgcag gaaacaaaga gaacttggcc ggacttccta gcgatcgcgt
     8521 agaattggtc gtaggagatg ttgcagatgc agaattagtg gaccgtctag taaaagaagc
     8581 ggacgcagtt gttcattacg cggcagaatc ccataacgat aattcattaa aagatccttt
     8641 cccatttgta caaacgaata tcattggtac gtatacactt ttagaagctt gccgcaagta
     8701 tgatgtacgc tatcatcacg tatctactga cgaggtttac ggcgatcttc cattacgtga
     8761 agacctgcca ggacatgggg aaggcgaagg agaaaaattc actgcggaaa caccatataa
     8821 tccttcaagt ccatattctt ctacaaaagc tggttcagat ctgttagtaa aagcatgggt
     8881 gcgttctttt ggcttacgtg caaccatttc aaactgttca aacaactatg gtccttatca
     8941 gcatattgaa aaatttattc ctcgccaagt aacgagtatc ttaagcggta ttcgtccaaa
     9001 attatatggc gaaggaaaga atgttcgtga ctggatccat acgaatgatc attcttcagc
     9061 agtgtggctg attttgacga aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg
     9121 tgaagaagat aataagaccg tgatggaatt gatcttagaa atgatgggcc aaccaaaaga
     9181 cgcatacgac catgtaaacg accgcgcagg gcacgatttg cgttatgcaa tcgattcgac
     9241 aaaattgcga gaagagctag gttggcagcc tgagtttacg aacttccgtg atgggttggc
     9301 tgacacgatc aaatggtacg aagaacatga agactggtgg aaaaaagaaa aagaggccgt
     9361 tgaagcagca tatgccaaaa atggacaata atacagagga aatttcactg tattaatgga
     9421 aaaacacatg tgaggcaggc gctcgcctgt ctctttttta aaataaaaat caaggagcgg
     9481 ataaccatat gtttttatta actggaggaa atggacaatt aggtacagaa ttgcgccatt
     9541 tattagatga aaaaaatctg ccatatgttt caacagatgc aaaagacttg gatatcacag
     9601 atgctgagaa aacaatggct tatattacag aattaaaacc cgatgttatt ttccattgcg
     9661 cagcttacac agcagtagat aaagcagaag aagaagcaaa agaattagat gagaaaatca
     9721 atgtcgatgg cacaagaaat gtagcacttg cagcaaaagc tgtgaatgca caattcgtct
     9781 atatcagtac agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg
     9841 accaaacgaa tccactaaat gaatatggtc gtacgaaatt attaggcgaa aaagcagtag
     9901 aagagatttt agaagactat tatatcatac gaacttcttg ggttttcggg atctatggac
     9961 acaatttcgt cttcacgatg caacgcctag cggaaacccg cgacaaactg actgtagtaa
    10021 atgaccagtt tgggcgacca acttggacac gtactttggc agaatttatg gtatatgtca
    10081 tcgaagaaaa agcaccattt ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt
    10141 atgagttcgc aaaagaaata ttgaaagata cagatgtaga agttgcacct gttacgtcag
    10201 aagaatttcc gcaaaaagca acacgtccgc agtattcagt catgagcctg aaaaagacag
    10261 aagcgttagg ttttgtcata ccaacttggc aagaagcact tgcgcaaatg ctggaagctg
    10321 ccaaaaaaat gaataagtaa tttccttttt atcaaataga atttggagca tgatatggaa
    10381 aaacaaacaa gaagtgcacg acgaaaagaa aagccaaaag cgaatcaggc agattcgaat
    10441 ttggtgcttg acctgtttct aaaactccgc tatcttattg gcgttttagt catcgtggtc
    10501 atcgttactt ttaatttaaa cggaagttca cttggtgttt gggataaata tgtgtcgcaa
    10561 agagacgatg ggaaaaaaac tgatgtgatt tttggacaga atcgggaagt gcggtcagac
    10621 gaatggctgg tgcagacacc tttttacctc tcacaagctg aagaggactt ttcgttggtg
    10681 aacgaggatt attcattaaa cggacaaaac atgatcattg cgtataattc tcctgtaaag
    10741 gatattacgg ttatcggaaa accattcaac tggggattct ttttcctcgg aagagatcga
    10801 ggactttcct tttattgggc gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta
    10861 ctgatgatat taacgaagag gaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca
    10921 ttttctccag cagtccagtg gtggttcatg caacatgtgg gcgatttgat tttctttact
    10981 ttgggactga tgattgcttt ttatcattat ttctacaatc atgataaaaa atggctacga
    11041 acgttgatga tgcttttggt ggttattttc ggtattgggt ttattttagt gatttaccca
    11101 gcccatcaag tgatgctggc gtacttgctg attttttatt ttgttggcct gcttatttac
    11161 tatagcaaga aagtttcatg ggatctgttg gatgtagtgt tgattggagg ggcagtttta
    11221 tttattggcg gagtcatggt tcatttctgg ctaacgtcaa aagacgcatt gttagcttct
    11281 ttgaatacct tatatccagg aaaacgggta agtactggag gaaagtggac gattggcgaa
    11341 tttttttctt tccttacaaa ttggaaaatt ccttttgaag atatcaacta ttcgaataac
    11401 tctgaagtgg ccctgttcta tcactttttt ccaactgtat tcctagcaag tccatttgtg
    11461 ttatttggca aaaaagatag cgaacaaaag ttactgggac gtattttgat gattttttgt
    11521 ctgttttcta tcttttggat taccgttggg ttgccaaaat ggctagcaga aatcactctg
    11581 ctgtcttatg ttcctccatt aagagccatc cttactttta gttttgcggc ttgtttatta
    11641 tcgatctggt ttatcgctta tatttggaga gaagatgtta ttcctatttg gtataaattc
    11701 attttgttgg cagtaaatgc atggtcttat ttctatgcga ttacgcattc gaaaatggat
    11761 aattattttt cggattttga aaccatcgca gttgctgtgt tgggaacagt gattttagct
    11821 tttgcattat ttggctggag acgcttgttt tacctcgctt ttgtcgcctt agttatcgta
    11881 tctggctttt tcgtcaatcc agtcgtagaa ggtacaggag cgatttttga aaaaacactg
    11941 gcaaaagaaa tccaaaaaat cgatcaacaa gatcctggta aagtttggtt gtctgaagat
    12001 gaattatata acttcacccc aacattggga gtgaaatcct tcaatacagt acgcttttat
    12061 ccagatatag ctgcatggaa gaaaatcgat ccgaaagaag aatacgagaa gtattataat
    12121 cggtatgctc atacaagagc ttttatcgct aaggaagata caaagttcat tttagatcgt
    12181 ccagatatgt tttcggtcac gttgaatttt gaagacgcag aaaaattagg gatcgactat
    12241 gtgatcagta aacgaccatt aactgattac aaccaattat atgaagcgca gttcacgcag
    12301 ctatatggac cggataaaga cggttatatg atttttaaag tagaatatcc ggattatcca
    12361 gatgtaccac aaacacaaac tcaggaaaat cagctagtcg tacagtaata ggctagctga
    12421 gaataatcct gaaatattag tagaaaggag agtgaagata attcaagcaa tcgtacaaaa
    12481 atttgtcaac aaaatgagtg tcggtttctt gcttttgttg ctgacgctag tcttttttgt
    12541 ttggacagca ggggtaggtt ttccagcaac tttgtttata tataacaata gttcattact
    12601 tatcgctttt agtgtggcta ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga
    12661 ttttctttat ctaggtgttg cgcttgccct tttcattttc ttttcttact ttcgggaatt
    12721 aagaaatgag tctttatttg gagacttact gtttcctttg gttattttat tttgtttagt
    12781 aattaaatgg atcgagtgga caaaatggga tcgcggtatt tatctgattg tctttacggt
    12841 cttcctcgga atcacgttat atcgtgtatt tacagagatc caagtaccag aaggacaaag
    12901 catctgggct ccaggcaatt cgctttctga tatttggatc aatacgaata caatcggaag
    12961 ttctttgatg actcttgctt tattgatcag tggatttgcc agttcttttg aacgatggta
    13021 tgttcgaatc atcggtggga tagcagtcgt tgcagccttt ttaggtattt gggtctgcca
    13081 atctagaggt gcattagtcg cgttgattgt ttttgcgata ttggatcttt tacctaaatc
    13141 gttgatgcgt gtcattcgtg cgccatttat tgcctacacg gttacgattc ttttagcttt
    13201 gccgatttct tatctggcgg ctgtctctga aaaagtcaat ttgtttaccg atcgagaaga
    13261 tatttggcat aaattctatc agacgctagg agaaaaatct gaacaagttt tactaggaat
    13321 gaaaacattt atcttccagc gaggaaacca atttttagga aaccacaata gttataattc
    13381 tattttgaat atctatggat tgataggatt tgggattgct gctttgctgc ttatcctttt
    13441 tattggtcgc ttaacgttga aagcagattt atccaacggg cagatgactt ttatctgggc
    13501 cttttttgcg gtgatgatgc agtcatttat ggaggataca ttgacttcat acccatgggt
    13561 tccgattatt tatatcctgt tggctatggc cgttcatcga tatgatgaac cagaagttgc
    13621 ggaggaattc tatgttatgg aagaggcata tgacgatatg acttatgatg atacatctta
    13681 tgaggaacaa ccagatagcg ctcattcttc caggatccaa cgctatcatt gataggcaca
    13741 aaatacgaga gaggctgtga caaccgtcgc agcctttttt ggtttattgc aattgtctag
    13801 catcttctgt aaaatagaaa cgttagagaa aatcttgtat catagaaaat taagaaagaa
    13861 ataagaacgc agctcggtac caaaaatatg gtaaaatggc acttgactgt aattcactga
    13921 aaatcgcttg gaggaacaca aatgtttaaa gatttgtatt tatttatgaa ggacatttat
    13981 cagaacagaa aattattgct acagttttcg atcaatgact tcaaatcaag atatgcagga
    14041 tcgtttttag gaatcttatg ggcgtttatc aatccgctat ttactgtatt gatttattgg
    14101 ctggtatttg gtgttggact gaaagcacaa atgatcgatg caaaataccc atttatcgta
    14161 tatttagtta ccgggatgat tccctggttc ttcttctcag atgcatttgc atcaactacg
    14221 gttgtttttc gggaatatac ttatttagta aagaaagtcg tattcaatat tcgtatcttg
    14281 cccacgacga aaatcctatc caatctgtac acccatttat tcttcattct gattggcata
    14341 gctgtcgcgc ttataaatgg tatatatcct tcagcaatgt ctttacagct gatttattat
    14401 ttattttgca tgtgtgcttt cttgacagga ttgacttggc tgacggcatc gatccaacca
    14461 tttttacctg atatcatgca attaattacg atcgtgctgc agttgatcat gtggacatta
    14521 ccgatattat ggagccctaa tcagtttgat tcaggtcttg taagcatatt gaaattaaat
    14581 ccgctgtact atgttgttca aggttatcgt gagtcatttt taagtgaagc ttggttctgg
    14641 gagcatggaa catatacttt gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc
    14701 tctgcagtgt tccgtagatt gaaaccacat ttttcagatg tgctgtaagg agaatagcta
    14761 tgacagaata tgcaattaga ctaaaagata tcacaaaatc ttataatatg tacgcaaaac
    14821 cttcggatcg atttcgtgaa gccctcaatc cttttaagaa atcctatcat gatgtatttt
    14881 atgcgttgaa aaacgtgaat gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag
    14941 aaaacggatc gggaaaatct acaatgttga aaatcattac gggagtactg acaccaacat
    15001 ccggcacgat ggaaatcgaa ggaaaaatct cagctttgct agaactagga tcaggattca
    15061 atcctgagta ttctggctat gaaaatatct atttaaatgg aatggttcta ggattttcaa
    15121 gagaagaagt cgatgaaatg gtcgatgata tcattgaatt tgctgacatc ggagaccata
    15181 tttatcaacc tgtaaaaact tattcaagcg ggatgttcgt tcgtctagct tttgctgtag
    15241 caatcaatgt gaatcccgat attttgattg tggatgaagc attggcagta ggcgatcttg
    15301 aatttcaact aaaatgtatg gaaaaattta cagaaatcaa gaactcggga aagacgattt
    15361 tattcgtttc tcatgatatc agttcgattc gtcgtttttg tgatcgttcc tattggttga
    15421 aaaacggaga agtcgtcgaa tatggcgata cgatggaagt aacgacgaat tatgaaaact
    15481 ttttaaagaa aaaatcagtt aaaacagtgg atcggaataa aaaccaagta gaacggttcg
    15541 ccgctccaga tatcgtcgac gtgacgaaag cggaattgtt ggataaagac aaaaatccgg
    15601 tggatatgct ggaacaagga gaaaaactat acgttaaagt aacttatgaa gtaaaagatg
    15661 atacaatcaa aaatccggtt ttaggaattg cgctacgaac agtcgacaat ttttatgtat
    15721 gcggacttaa tactctacta gatggtatca agattccttg gaaaaaagga gagaatatct
    15781 tttatctgga gtatgaaaag ttaggtttgt tagctggtga atattatttt gacgttgcgg
    15841 tctttgaaga aaatgcaacg gttcctttgg tatataagac aaagtacatg aacttgtttg
    15901 tcagcggttc ttatatcgga gaaggcatcg tggtcttaga ccataaatgg gaagaaggga
    15961 ctcatagcaa tgaaatatga ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa
    16021 attgccgcac agatcaaacc aggaagtaaa gttttagagt ttggtcccgg aaacggccga
    16081 ttaacgaaac atttaatcgg agcaaaacaa tgtgaagtga gcattgttga gttagataag
    16141 gaattgttcg attttgtcag cgaatttgct caagatggat tttatggtga tatcgaaagt
    16201 tttgagtggg caaactatta tgctggacag acatttgatt acgtcttgtt tgcggatgtt
    16261 ttagaacatt tggtagatcc tggaaaaaca ttgaagaaag tcagagaatt tttgaatgaa
    16321 gaaggagaga tcctgattac attccctaac ttggcgcata attctgtcat gatcgatttg
    16381 ttcaataacc aattgccgtg ggcttcttat ggtctcctag atgaaaccca taattctttt
    16441 tatactcatg atggcttcca aaaagttttt gaaaaagcgg gattattcat taatattgaa
    16501 gattatttgt acttagctgt aggtgataca gaattaaaat caacttatga agaattgccg
    16561 gaagctgtac gctatgattt caaaatgcga ccatttggtg aagtctacca atactttttc
    16621 tcattgatga aacatccagt agcacaaagt tccattgcag agccacaaaa ttctaattat
    16681 gtaaaagtgt tagaagtaac ccagcaaacg aaacaagatg aaacaatcca acaaatacca
    16741 ttcaataatt tcacagggga aaatgagaca ctaagtttcc ccgtatcaga aacaacagaa
    16801 agaatggtgt ttcgcttcgc gcaacaacct agctttatcg aattcagcgc agaagctgcc
    16861 ggagaaaaac taacatttat cgattcgaat gctgtagtca aaacagtaaa tgattgttat
    16921 ttgtttgatg ggaaagaatt accagagttc gttctaacgg atatttctgg gaaagaagtg
    16981 actatccatt gtcactatcg ctttatcggg gaattgacac caacaatgaa agaattgttg
    17041 gaaacgattc gtcccatggc tgaagtcaca aagcagcttt ctgagaaaaa tgacgaatta
    17101 gaaagagaaa atcatcatct tcttgaggaa aatacaagac tagaccatac gttaaaaaca
    17161 accacaaatc gctattgcac attgttggaa agtgatgaat ggacaataaa acatagacta
    17221 ggacgacgta agaaagaaac ttcgaaaaaa atccaagaaa aagaactttc tatatgtatt
    17281 gacgagaaaa tctgggatgc agaaacaaaa atattaaaaa tcatcggatg gggaatcgct
    17341 aattcggatc gccagccctt gtcctataag ttatcagctg accaatcgcc atttttcgaa
    17401 gcaattcctg tttcacgaga agaagtcaat caagcagaac agctcgcaaa aggaacagaa
    17461 gcgggatttg agttacgaat cctttgcgag caagaacgtt cattcttagt ggaagcagta
    17521 gctcaaaatg gccaatcatg gatcattgaa atgtaaataa tgattaactt ataaagataa
    17581 ggagtaaacg aatcgtgcaa aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg
    17641 aacgggccac tggcgatttg cttattgtgg gctgggcaat tgatgaagtg accaaagaaa
    17701 tccctacgat aaaggtagaa aaagaaaatg tgattgctga agcaacacat gtcgtaagat
    17761 tggatatcaa tcatttatat aatcttgatg ttaagaccca aagcggtttc aaggtacgtt
    17821 tatcagggaa aatgagagga aaagccatct tagacttcca aacagcgaga catcaaaatg
    17881 gcatagctgt gaaattaaac ggtaaatacc cttatgatga cggcattgaa tcttcatggg
    17941 aaagaaaaaa aagattactg aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa
    18001 aagcgatccg tcgggtcaaa ttggaattga agccaggatc gatcgactat gcagagtgga
    18061 tcagtcgtca tgagaaacca gatttgaaag agcaaaggaa agaagcacaa ggatttgcgt
    18121 accgtccatt gatttccata cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa
    18181 aatgcattga ttcggtactg gatcaaacgt atgatcattg ggaactatgt atcagtgatg
    18241 atgcttctac tgatccagct atccgaaagt gtctggaatc ctatcaagca aaagatgata
    18301 gaatcaaagt cgttttccga aaagaaaatg gacacatcag tctggcaact aattctgcat
    18361 tggaaatagc agaaggagaa tttatagcac tcttagataa tgatgatgaa ttaccgccat
    18421 ttgcattata tgaagtggca aaagtgctaa acgtacatcc agaactggat gtgatctaca
    18481 gtgacgaaga taaaatcgat gcagatggca atcgctttga tccgcatttc aaagcagact
    18541 ggtcgcctga tacattgatg ggaaataatt atatctctca cttaggcgta tatcgaacaa
    18601 gcattgttaa agaacttggc ggtttccgta aaggctatga aggttctcaa gattatgatt
    18661 tggtccttcg tgtgacagaa caaatccctg cagatcatat ttaccacatt gaccgagtct
    18721 tgtatcattg gcggacgatc ccaggatcaa ctgcaagtaa tggcgaagcc aaaagttata
    18781 tctatgattc cggagtgaaa gcattgacag atgccctgtc tagaagaaac atcaaaggaa
    18841 gcgtacgtcc tggcagaatt tctggatttt atgagattgc ttatgacgtc ttgcaagagg
    18901 atttggtcag tgtgatcatt ccaacgaaga atggctatga agatctaaaa acttgtgtgg
    18961 attcgatcat cgaaaaaacg tcttattcaa attatgaaat cattattgcg gataacggaa
    19021 gtacagatcc aaaaatgcaa gaattatttg cagaatacaa gcatcagctg aaagaccgct
    19081 ttatcgtaga attgattgac attccattca attattctcg aattaacaat ttagcagcag
    19141 aaaaagcaaa cggcaaatac tttttattct tgaataacga tacagaagtg attgagccag
    19201 attggatgac ggtgatggtt tcttatgcac agtttgatcg aatcggctgt gtaggagcaa
    19261 aactgtttta tccagatgat acaacgcagc atgcaggagt attattagga atcggtggtg
    19321 ttgcagggca tgcattgaat aactatgatc gaacacattg tggttatttc ggccgattgg
    19381 tcattgacgt caattactta gcagtcactg cagcttgtat gatggtcaag gcagctgact
    19441 tcaatgctgt taacgggttt gatgaaacat tagaagtagc attcaacgat gttgacttgt
    19501 gcttgaaagt ttatgaactg ggtcgttaca atgtctatgc acatcaagca gaattgtacc
    19561 atttcgaatc taaatcaaga ggttatgaag atactccaga aaaacaaaga cgttttgctg
    19621 gtgaaatcaa aaaaatgcaa gataaatggc cagcatatat tgcgcatgat ccattttaca
    19681 atgataattt aacaaaagac ggaatcggcg acttttcgtt gagaccagat taaactataa
    19741 taaaaagctg ttatgttgtg attcgctcgc acataacggc tttttatagt tttttgtcaa
    19801 aaaaaggcag aaggtgccga ctcaaaaaaa taagtcgagg ttgggacaga agtgtttaac
    19861 tccgagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcggctt
    19921 atttaagaag aagttgcttc tgctcccacc gtttattcgt ttttaaagaa tgagacaaaa
    19981 gtgtttctta cttttgtccc acgctcttat tcatgctcag agcgccaata gatacaaaat
    20041 catagccaaa gtttcacgaa ccctagctgt caaagaatcg atattttatt ttaatatagg
    20101 ctattttaaa ataaatggtt taaggtattc ctctacaaaa gccactctat caattatcat
    20161 gggtttaaaa taagaagtga tggagataac cagattgttc ttagcgtcaa tataaagaac
    20221 atttccacta ttaccaattg ctgaatatat ttcgttctca gaatctaata cccaccataa
    20281 atagccatat ctcatatttt gtaagtcttc taaagcactg gttctaggtt tgaacatctc
    20341 gtttatccac atgggtggta cgatttgttt tcctttatac atccctttat ttaggcataa
    20401 tacaccaatt tttgccagat cacttgccga taaacacaaa ccatatcctg ccgtcccgat
    20461 atttagtgga tcacaaaacc agacattggt tttaggagac ttatccattg taaactgctt
    20521 atgttcttca gctgtttcag cataataatt gacatggttt ggtatcccta taggatcaaa
    20581 tagatactta ttggcaaaat caacagttgt tagcttactt gtctgataca acaagccagt
    20641 taatatgtgt aaacagacag tttgatagtt gaattcgccg gttatacctt ttcttcctcc
    20701 caataaatct agtgatgttt ttgtccaatc ttcacttgaa cagacttttg tccatggatc
    20761 gcctttacat ttgtagggtg ctttcattgt tagcaagtgc ttgattgtaa tagcgtaaat
    20821 agttttctcc ccgcgtttca ctttatactc tggaaaataa tctaatactt tatcatcgat
    20881 acttttgatt aatccttgct ctaaagcaat gccaatcagc aaagatacaa tacttttcgt
    20941 tgctgacatg atatgacaag tatcgtcttt tttataacca ttccattcat ctgtataaac
    21001 ttctacccca tccttatatg cgacaatttg acatatattt ggttgtcgaa aaccaataaa
    21061 atcatgcaac tcttgtttat tcataagata ttacctcact ttcttctata agcataatgt
    21121 cattaaaaat cgatttcaat tttttcgatt cttcacgtaa tacaggtatt cttatggact
    21181 agacagcgct ttgggaagat ggattctctg aaaaatataa aggttcactt tatgagtgga
    21241 taaaaaaagc agcaaatact tcgacttctt taatttacca acattagtga aaatcgattc
    21301 atctggagat ttgtaattta gagtccttgt cttgattaga ggtgtataaa gctttttatt
    21361 agacattttt aaattccatt acaatttgct tataaactag gtttttaaag gtgactatgg
    21421 taagataata gagatattat tttgtgatta ggagtgtata tatgaataaa aatggagaat
    21481 ggagtgctgc acgacggata ttcattatat taatggatgt tattgtatac aatgcagcaa
    21541 tatatagtag tttcttactg aagtttcgtg gagagatccc attacgaaat ttagaaactt
    21601 tccaacactc agcaattatt atttcagtga tttttattgc gttgaacatt ttgttgggtg
    21661 catatgtttt ttataatcga atgatcagtg acatcgtgtt tgtcacagtc atcggtcagg
    21721 tattgatgac actagggatc atggtagtga cttttgcggg ccgctggttt gcttttccac
    21781 gtcttgttat cttactttca tttgttctcg gtacgctttt cttaagtatc tatcgtattc
    21841 tcgtatataa actttattta cgcgtaagtc gagataaaaa ggtcgtcatt attgggttag
    21901 aaaaggatgt cgcaccagca atccaaaatt ttggttcaaa gaaaaacaac aaacataaag
    21961 tcgaagcagt cattcttgat aactattatg caaacatcaa aaatatgatt aaagacatcg
    22021 atattgtcta tcttgcttct catatcgaag aaactgaaaa aatcaaaatc tatcaattag
    22081 ttactaaaaa tgaaaaaaaa ttgtttttga atacaacatt tgaaaacttg acattgatca
    22141 atccaaatat tatgaatttc gaagatgaaa gtattattga agcatctgga tttagaatcc
    22201 aacctgaata tgaactattc aaacgtttgt ttgatatcat cgtttcactt gttttattag
    22261 ttattgcttc accatttatg ttattcacag caattttagt caaagtgaca tcaccgggac
    22321 caatcatcta taaacaagtg cggatcacaa aaaatcaaaa ggaattttca atctataaat
    22381 tccgctcgat gaccgccaca gccgaagcaa aatctggacc agttttagct aagagcaatg
    22441 atgcacgagt aacgcctgta ggtaaattta ttcgagctgt ccgctttgat gaattaccac
    22501 aaatcttcaa tgtgctgaaa ggcgatatgt cgatcgtagg accacgtcct gaacgttctt
    22561 tcttcgtcga tcagttcaat gcagaaaacc cttattacta cttacggcat aatgttcgtg
    22621 ctggtatcac tgggtatgca caagtctatg gaaaatatgt ctctgattac aacagtaaat
    22681 tacgttttga tttactttat atcaaaaaat attcattgat gcttgattta aagatcttgc
    22741 tgcaaaccat caagatccta tttgataaaa tgtcctctca aggacttgaa gaagacgaaa
    22801 atgtgatctc attggcagaa attgcttttc cgaatgaaaa aatctttcgc tagttgggag
    22861 gcgtaaaaat gaacaatccg aaattcagca taatcgtacc agtatacaat gcagaagcaa
    22921 ctattggtcg aacgattgat attctaaaag aattggatta tgaagagtac gaagtcgttt
    22981 tagtaaatga tggatcaaca gatcaaacac aggaaatcat tcaagaactg attgaaaatg
    23041 atgaacgttt tgaattgatt actacgacca atcaagggcc cggattagca agaaacgaag
    23101 gaataagaca gtcaaaagga gaatatctct tattcttcga tgcagatgac acgccagtaa
    23161 aaacgatctt gtcagattat gatcatttgc tagaaaagag tccaaaagcg gatcttgttg
    23221 ttagttcgtt tgtttttcgt acaatggacg agggaagaat cgtttccgaa aaagaaaact
    23281 tagttacaga atgtcactat ctaactcacg aagagtttat aaaagacatg tatcagttaa
    23341 tgaatgaaca gttgatgtat gttgtatgga acaaatgcta tcgtagcgat attctgaaaa
    23401 ataaccaaat tctatttaaa acttataata gttgtgagga tcgaatattt aacttacatt
    23461 attatgaata ctgtcatcaa gtgatcatga acccaaaagt agaatatatt tatgaatttg
    23521 aaggtgggaa ggggatcact aatcaatacc gacctaacaa gttcgaaaca tttaaagaat
    23581 tctattcatt agcaaatact gtaacagacg atattaataa agacggtatg gcggcgcttc
    23641 ttttaaaagg gacaacttca gttatttttt ctatttatag tacctctgca agaacttcaa
    23701 aagagaagaa agacgaagca aagcagatat tgagtgatcc agtgatccga gaagccaagc
    23761 aaattgctaa gacggattct tcagcaaaaa aaataacaaa gttgctttac aatatgccag
    23821 ctccactatt tttatctgct gtaaaagttg gaagcttcgt tgaagtgaaa atgccagggt
    23881 taatggctgc tttgaagcga gtatattagt taatggagga aaatataatg aaaagaattt
    23941 tgcattttca gggacgaatg ggtttaggtg gagcagagtc tttcatgatg aatttatatc
    24001 gtaaaattga tcgtacaaac tatcaatttg attttttgat ttatgaagat tatgcagatg
    24061 ttcaagacta tcattcagag attgaaagat taggaggccg tatttttgtt gtacctaatc
    24121 ctaagaaaaa tatttttaaa tatttaatag aagtgaataa gttactaaaa aaagaatctt
    24181 tttctatagt tcacaatcag gtttattttg gcgggggaat taatttatgg ttagccaaaa
    24241 agaatggaat cagacagaga attgctcata gccatgcaac tgaagatgga aaatctcaaa
    24301 atattgtaat gaatgtattg cgtaaatttt taaccaaatt gttgttacaa aatgcaacag
    24361 actaccttgc cgttagtcaa gaagctggtg aaagtctgtt tcaaaaccat ccctttgaga
    24421 tagttcataa tggtattgat ttagagcttt atagtaaaaa ttcagaagct aaggttaata
    24481 agagaaaaga attggatatc tcaatgagta cttttgttgt gggaaacatt ggacgattag
    24541 agaaacaaaa aaatcaatct tatttgttag agattttttt agaaatacta aaaatgaatt
    24601 ctgattctta cttgatttta gtaggtgaag ggtcacttag aaataaactg aaagccaaag
    24661 cagtagaatt ggatatagag aaaagagtaa aatttttagg agaaagaaac gatattcccg
    24721 agttattatc tgtgatggat gtttttgtaa tgccttctct ttatgaaggt ctgccaatgg
    24781 ttggattaga agcacaagca gcaaaattaa aattagtctt agcagatact atttccaaag
    24841 atacaaagtt aacaaataat gtggattttg tatcattaga tgaaacacca caagaatggg
    24901 caaaaaaagt aataaaagag ccgtatgcta ataaaataac acaagaacta aagagttatg
    24961 atgtttcgta cactactgaa ca
//
LOCUS       DAPCMU010000025        24932 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPCMU010000025 DAPCMU010000000
VERSION     DAPCMU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.025"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(368..1486)
                     /locus_tag="RD744_000861"
     CDS             complement(368..1486)
                     /locus_tag="RD744_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556887.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1957..3264
                     /locus_tag="RD744_000862"
     CDS             1957..3264
                     /locus_tag="RD744_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDU0556888.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="RD744_000863"
     CDS             3383..4582
                     /locus_tag="RD744_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDU0556889.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="RD744_000864"
     CDS             complement(4609..5877)
                     /locus_tag="RD744_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDU0556890.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6040..6686)
                     /locus_tag="RD744_000865"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="RD744_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="RD744_000866"
     CDS             complement(7094..7597)
                     /locus_tag="RD744_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0556891.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="RD744_000867"
     CDS             complement(7720..8484)
                     /locus_tag="RD744_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDU0556892.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="RD744_000868"
     CDS             8591..8866
                     /locus_tag="RD744_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDU0556893.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9582..10535
                     /gene="yidC"
                     /locus_tag="RD744_000869"
     CDS             9582..10535
                     /gene="yidC"
                     /locus_tag="RD744_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDU0556894.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10639..11866
                     /locus_tag="RD744_000870"
                     /pseudo
     CDS             10639..11866
                     /locus_tag="RD744_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12176..12397)
                     /locus_tag="RD744_000871"
     CDS             complement(12176..12397)
                     /locus_tag="RD744_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0556895.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12558..12956)
                     /gene="tnpA"
                     /locus_tag="RD744_000872"
                     /pseudo
     CDS             complement(12558..12956)
                     /gene="tnpA"
                     /locus_tag="RD744_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13170..13536)
                     /locus_tag="RD744_000873"
                     /pseudo
     CDS             complement(13170..13536)
                     /locus_tag="RD744_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13550..13835)
                     /locus_tag="RD744_000874"
                     /pseudo
     CDS             complement(13550..13835)
                     /locus_tag="RD744_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13841..14035)
                     /locus_tag="RD744_000875"
     CDS             complement(13841..14035)
                     /locus_tag="RD744_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556896.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14035..14511)
                     /locus_tag="RD744_000876"
                     /pseudo
     CDS             complement(14035..14511)
                     /locus_tag="RD744_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14979..15332)
                     /locus_tag="RD744_000877"
     CDS             complement(14979..15332)
                     /locus_tag="RD744_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556897.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15460..15999)
                     /locus_tag="RD744_000878"
     CDS             complement(15460..15999)
                     /locus_tag="RD744_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556898.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16029..16220
                     /locus_tag="RD744_000879"
     CDS             16029..16220
                     /locus_tag="RD744_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556899.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16262..17128)
                     /locus_tag="RD744_000880"
     CDS             complement(16262..17128)
                     /locus_tag="RD744_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556900.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17152..18804)
                     /locus_tag="RD744_000881"
     CDS             complement(17152..18804)
                     /locus_tag="RD744_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDU0556901.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18809..19711)
                     /locus_tag="RD744_000882"
     CDS             complement(18809..19711)
                     /locus_tag="RD744_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDU0556902.1"
                     /translation="MMNLQKELKCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19795..21108)
                     /locus_tag="RD744_000883"
     CDS             complement(19795..21108)
                     /locus_tag="RD744_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDU0556903.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21176..22120)
                     /locus_tag="RD744_000884"
     CDS             complement(21176..22120)
                     /locus_tag="RD744_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDU0556904.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22157..23170)
                     /locus_tag="RD744_000885"
     CDS             complement(22157..23170)
                     /locus_tag="RD744_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556905.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="RD744_000886"
     CDS             complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="RD744_000886"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDU0556906.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            complement(24568..>24932)
                     /locus_tag="RD744_000887"
     CDS             complement(24568..>24932)
                     /locus_tag="RD744_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0556907.1"
                     /translation="PLTEVEIFHTDRGKEFDNQAIDELSTTFDINRSLSHKGCPFDNA
                     VAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNRLRLHGTLGYETPVGYRNQRL
                     AQRILDNELGCANASEAV"
BASE COUNT         7092 a         5642 c         4749 g         7449 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9481 gaacgaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9541 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
     9601 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
     9661 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
     9721 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
     9781 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
     9841 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
     9901 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
     9961 tatatcgcga aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10021 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10081 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg
    10141 gcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10201 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10261 cagccggtgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10321 tcacaaacgt tataatgaaa ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa
    10381 acccgccaaa acaagtcgtt actccaagaa aagacgtgac agaatcagca gcaaagccag
    10441 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    10501 gccgcaacgc aggaaggcag cagaagcgga aataaaaata tatgacaaaa aactgtagac
    10561 aaataaaaaa atttctttgt tctacagttt ttttgttaca attaaagaaa acgttcgcat
    10621 ttcgaggagg aatgtataat gatgtccaaa taatcaatgt atagtcgaat ggaagaagac
    10681 aaaatttcgc tagatgcact tgttcctaaa aatcatttag tccgtaaaat tgacaaatta
    10741 ttgtcatttg attttattta tctcattgtt gaatcaactt actcgaccat tggtcgccct
    10801 agcgttgatc ctgtcgtctt agtaaaatta gtattcattc aatatttatt tggaatttgt
    10861 tcgatgcgtc agaccattaa ataagttgaa acaaacctag cttatcgttg gtttcttggg
    10921 cttagtttaa ttgataaagt tcctcatttt tcaacttttg gtaaaaacta tgcacggcgt
    10981 ttcagagaaa ccgatttgtt tgaacagata tttatcatta ttctcgaata agcagttgat
    11041 gctagattta ttcatgaaga aattttgtat atggattcaa ctcatattaa agcgaatgcg
    11101 aacaaacgta tttttacaag agatatggca cataaagaag ccattttata ccaaaaacaa
    11161 ttagaagaag aaatcaatga ggcaagaatt gccgaaggca aacgtccttt tggttcaatc
    11221 attcgtcagg aggtggtcga aagaaaattt agcatcgcag atccagaaag tggttattat
    11281 gtaaaaacag aacgagaaaa acagtttgct tattctgctc ataccgttgg tgatgaaaat
    11341 ggttttgtat tagatgtcat gattacgcct ggaaatctac atgatagccg aatgctcgtg
    11401 ccacaaatcg aacgcgttaa atcacgttat ggtgtagctg ctaatgctgc gtacaaaaca
    11461 ccttggaacg tcaagtattt aatcgaacga aaactacgac ccattctccc ttatatcagt
    11521 ttcgcacaac tactcgagat ggctataaaa aatatgtatc taaatctttt atttgtgaaa
    11581 gttgtccatt attgaaaaat tgtacagaaa gccaaaaaca ccaaaaagaa atacatcgac
    11641 atatttggga agactactta gatgaagcag aacatttacg ccatacggtg tacaatcgtg
    11701 aaaaatataa aaaaagaaaa gaaacgattg aacgcatttt tgcagacgca aaggaaaaac
    11761 atggaatgcg ctacaccaag tatcgaggct tggaaaaaat caggtgcata caatgcttgt
    11821 ttttgtcgca atgaatctga aaaaattagc tacttggcag tgggagcata tgtcgttata
    11881 tcttttatta tacaaaaata gacaaaagta tcagtctaca ctcttagtaa agaggaaact
    11941 gatgcttttg tctacagtct tagagagtgg gacaatagtt ttttcttctt tacgtctttt
    12001 cctctccatg tattttccta attttcttaa tttaacgcca tgagtgccaa gccaatgtca
    12061 atcttggctc cttgtttcct tcataagtgg aaatccgttt ccgatagatc gaaccttctt
    12121 tttcctctaa aagttttttt acattctgct ttgaaataac gccatgtatt attcactaaa
    12181 atttgtcggt ttcgctcact ttttgctttc gtacattgac tacgaacagg tcagcaccga
    12241 ccactttcac attcgtaaat tttgaagaca cgtataaatc cacttttatc ttttcttcga
    12301 ctaaagcgtt gaccgcaaaa gcgacatctg tttcagaaat atattgttcc atatctaatg
    12361 gtaaaatttt ttggttgatg gtataatttt taaacataaa gcactcgctt cctttctgat
    12421 tgggtttggt cactttaatc ctacagggat gagtgctttt tgtgtacctt aaaatcagta
    12481 aatatccatc cgtgaaaacg ggtggctttt taacagccct agaagggctc accactcaca
    12541 gccctatctc tctttaacta tttaaatgga tctacatact cttgtttact tatactgtct
    12601 ctaattcgat catctgcttc ttgttctcaa atatatattt caattgtttt ttgatttaac
    12661 ccaaccgtac tcacgtagta tcctttggtc caaaagcttt tattcccata cttatatttc
    12721 aaatttgcat gtttttcatg gattaaggtt gcacttcgtc cttttaagta gcccataaaa
    12781 ctagaaatac taatctttgg cggtattctt actaacatat gaatgtgatc aggcattgca
    12841 tgagcttcaa tgatttcaac atctttcctc tcacacaatt ttcttaatat ctggccaata
    12901 cttactctta actttccata aatcactttt cgtcgatact tcggggtaaa gactatgtga
    12961 tacttacaat cccatctcgt atgtgctaaa cttttatcgc taaagctatt tcgtccacgc
    13021 gtagaaacgc gcgttttttt gtttcgtacg ctacgccaac gaaactgact aaagtcaata
    13081 aaaaaagtgg ggcaataatc agattttttt ctgactattg tcccactctc tttttattac
    13141 tcttcatctt ctactattcc actaattaat tagttgacag atacaatgct ttcacatcct
    13201 gcacgaacaa actcgggctc tgaaccaatc acttgcgagt tgataaattc aatgaccata
    13261 ggaagattaa tccgacataa agtttaatct gttgtccttc cattatcagt ttagaaatga
    13321 tattagtagg tgtaccgcct aaaagatcgc atagtaccac aaaatcattt aacgaatcta
    13381 tgacagtaag agcttttgtc tgtatgtttc agtgccatct tctggtaata aaggtaacgt
    13441 gtagatattc tcttgaggtc ccataatcat ttttgtactt tttttcaatt cctcacaaaa
    13501 ttttccgtga ctaatcaata tcaattttct actcattttt ggctctccct tatttcgaaa
    13561 tcacacacaa tgcagataaa gccacacaca agtaatacga taaaaatcgc tcgagttggg
    13621 tcatattttt ctttcctaac aaccaataaa cacctcgacg atcccagcga gtaagatttt
    13681 agataatatc atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtac
    13741 cataaaaatt taatatcaat cattgttgca actaatgctc ctaccatgaa cactcttaat
    13801 aaagtcgcta cgtccgttaa tacagttaag cgagattaca ctagatgatc tgttcctgcc
    13861 aaggcaagag ccagaagcgg ttgcctgccc atttgccttt ggtgtgtcgg agctgtccga
    13921 taaagttcac cgcccgatcc gctctggctt tgtcgtagtg acattccgaa agcatgaatc
    13981 ggcttggtgt gtattccttt aatttgggat agccctttgg tctggtttct gccatcagct
    14041 accacctccc aacagagcct ccatcgcatc ttctgctccg acaccaccgc catcaccggc
    14101 aatgatacgg cttctggacg aaggcgtcag accgaactgc tcggaaaatc ggttcataat
    14161 tttcagatag gtctgggcaa taaacacctg tggtacctgc tgccaatagc cggatagggt
    14221 tttcacgatg ataccgtgct gggtaatgaa ttcctcggct tccttccagc gggcgtaggc
    14281 ttggcactaa ccggcaaagg cagccatatc cacatctgtc aagattccga tggcctccat
    14341 ctgctttgcc agacgcttcc attccttctt tgcctctggt tccagccact tcggacaggt
    14401 cggtgccttc ttttccggcg ttgactcttt tgcattgagt gggcgattgc ccggatttcc
    14461 ctccagcttc ttcatggctg tcggggttga ttttcttcct ctggttgcca cagtcgtcac
    14521 ctcccttcaa tgtatttctg ccagtagaca gagaacagag ccaagtcgaa acctgactct
    14581 cttctctatc tgctaacttg tatttcagtc cactcttctg atgaatcgca cccgcaggat
    14641 tttcaagatg ccgcaggcaa attccaagaa gtttctgacc gcatcataac catcgtagcc
    14701 gtgtagccac atccttccac ctcgccgccg tcctcgtcgc tgatataaat gattctgtat
    14761 tcgctcatct gttgttttct ccagtctttt gtttttttgt agtacatata tcactctaaa
    14821 agtgattatt atcaagtcgt ttgaaagcca taatgtacac aatcattcga ctggtttttg
    14881 gttcataatt gaccgccaaa gtgagcaaaa gaaagggacc tctgtctgtt tcagaagtcc
    14941 ctttcggtat ttggttctta gtggttcagg ctgtattctc aaacctcata ctcatcttcg
    15001 aggtctgcct ctgcaatgat ctggccgatg ctgtcgtaac gctggacctg catctggatg
    15061 gcatccttgt ctgctccctc gtccatcatc ttcagcagct tgtctgattc ctttttgtag
    15121 gcccagaaaa ggatctcctg aaggctggtc tcctgctttt tcttgcgaag ggcgaccttc
    15181 ttctcttcca ccgcctcgtt caacttggtg aactctgccc aagcaaggat accgtctcgc
    15241 ttgattgcga tctccacatc cttcagctcc gggatggtct ttgcaccctt gatctccttc
    15301 aaatatttct ttgccttttc gctcattgcc attagcgttt cctccttgtt tttgggattt
    15361 tcccctttgg ttgtgtgtat attgccatag tctctcgact atatcaagtc aatccacagg
    15421 catatactac acaatcattt ggagctgtat ttgtatacat tatgccgatt tatgagagaa
    15481 gaaaaggctc cagccgttcg actggaacct tgggggggtc ggtttcttag gcggcgtggc
    15541 ggtgtagacc ttgaggtagc tgatgttctt ccagacgtcc gtgcccttca gtctgaccat
    15601 gccgtccagc tctcggtcga gggtgaactt gcggaaaagc ttgatgcggt tgaggctgtt
    15661 tgctagctcc ttgccgttct tggcatccag aactgcgtcc tcgatctccg caatactaat
    15721 ttcctgtcta aaccaaaggt tgctcaaggc ttcgggaatg ccgtttgcgg tctgcagggg
    15781 gttgaaagtt tccaatacag caggggtcga aaccacctgt ccgagctgga attttgcgtt
    15841 ccctaaaatc atctgcataa tttcttccat gtgaatcatc catttctgcc gttgtggtat
    15901 tccctttggc agtacagtac tatatatcac tctgaatcgt acatttatca agaagcggat
    15961 ttttgtggaa tcaccaattc gatataatag taattgcatg aaaaatctaa gcactggatt
    16021 ggtaggtgat gattatacgt gacgatcaca tttacacctg tgattcctgc cactacagct
    16081 tccctgccga cgaacagcca gagcgttgtc ctgactgcga aaagacagca actcggctgg
    16141 ataccgaaat cgaaactgaa gattactatc gtgttcgtgc tgaaatcaaa gctgagatta
    16201 aagctttgaa tgcgggttaa cgacaggtac tgaccggcgg ctcaacaccg ccggcttttt
    16261 attaccacat cttgcggaat tcgctgggac gaacaccttc caccttgaaa aaagttctgg
    16321 aaaaatagtt tgcatctgtt atgccaattt ttgttgcaat ctcttccata cttagatttg
    16381 taaagcgtag caagtgcttg gcctgtgtga ttcgtttctg attcagataa ccgacaacag
    16441 ttgtaccgta agtttcacga aaaatcttag ctaagtactg tttattgata aaaaactctg
    16501 cactcaactg atccagcgta atcttctccg tgtaatggtc atccagatat tgttttatgt
    16561 tctgcaattc catttttttt gtagagaaat tcggcacttt gtcaggattc caagaatctt
    16621 ccatcaaatg catcagcagt tcactgagta gaacattgat tttcatatcc cggacatact
    16681 cctgtccctc tgccaccata tgaaggctcc tcagcaagtc agcatactta tctgtttttg
    16741 atgttttaaa gaccggttga ccacctcgtt caaggtattt tttgtagact tccgtcatac
    16801 caatgccatt aaaatggcac caccgcaaag tccacaaatc atcttctgat gactcatgga
    16861 catacggctt caaacagtca ataaacacca tatctcctac ttgaagcgta tatttctgcc
    16921 ccttatactc caatgtgcct gaaccaactt ctaccaggaa acaaaggtaa gatgacaatc
    16981 ctgtacgcct agatgtatgt gccttcagag cagtcagctg tccaatttcc tgcaaataca
    17041 aaagagagga tcttgcaaag aacgatgccg tgtacagcac acgatttgac ttcaccgatt
    17101 tcaaagcgtt agaaaacagt tcctccatta caagatttct cctttctttt cttactcgaa
    17161 catttccgcc agttcagcag ccataatagc ttccatagct tcggcggctt ccttgttctc
    17221 tgcgctgacg ctgatatagg tcttcagctt cggctcagta ccagaaggac gaaccacaac
    17281 agagcagtta tcttccagca taaacttcaa aacatcagac ttcggcagac cattcaggcc
    17341 cttactgtag tctaaagttt caataacctt cttaccacca attatatcga gacccttgtg
    17401 gaattcaacc ataatttcct gcatcttggt aaaacctgcc gcaccgtcaa actcgaagga
    17461 atgcagggtg ttcaaacaat agccatactt tgtataaatc tcgttcagct tatccagcag
    17521 gctgataccc tgagtcttgt agtaagagaa catttcacag attagataag cagcgttaac
    17581 accgtccttg tcacggacat agctaccgga cagatagccg taagattcct caaagccgaa
    17641 gatatagctg tcaaccttac cagccttttc caacatacca atctgctcac cgataaactt
    17701 aaagccggtc agcacatcga tggtacgaac accatagttg gatgcgatac gttcacccaa
    17761 atccatggtg acgatggtct tgaccatcac agggtcagca ggcatttttc catgcttaat
    17821 tctctgagaa cagatgtagt ccagaagtag cagaccagtc tcattaccag acaacagaac
    17881 atagtcacca tgtgcgctct taactgcgat accaacacgg tcgcagtcag ggtcagtggc
    17941 caacagcaga tccgcattgt acttcttggc atactccata cccagagaca tagcttcctt
    18001 aatttcaggg ttcggataag gacaagttgg gaaattaccg tccggcagac gctgttcttc
    18061 cacaacggtc acattgctgt atcccatctc tgccagggtg cgggtaacag gcaccagacc
    18121 ggtaccattc agaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag
    18181 gacggactgc cccttcactt cttcgatgaa agcagtcaga acttccgact gaatggatgc
    18241 aatcttagaa gcatcgccat ccatctttac atcagcaaag atgtccagct tttcgatttc
    18301 tgccagaatt gcagtagcag cctcggtagt aatctgacag ccgtctgcac cgtatacctt
    18361 gtagccgtta tatttagaag ggttatgaga tgccgtaatc ataacaccgg catctgcctt
    18421 cagataacga gtcgcaaaag atactgtagg aacaggaagc agttctggcc acaagtgcac
    18481 ctgaatacca tttgcagcaa agatgtctgc ggcagtcttt gcaaacacat cagatttgat
    18541 acgggaatca tagccaacca ccacggaagc ggcttcatag ttcttattca gatagtctgc
    18601 cagaccttgg cttgccttac ctacggtgtg tacgttcatg cgattagtgc ctgcaccgat
    18661 ggtaccacgc agaccaccag taccgaaaga caaattttta tagaaagagt cctcaatgtc
    18721 cttctcagaa taggaacgca gttcttctat aatatctgca ttggcatgat tcagccaacg
    18781 ctcatattct actttataat tcatattatt atcctctcac atccagaaac tgaatcgacc
    18841 cgtgaatgct gatttcagta gaatcggccg gcacaaaaag gcagtccccc ttatagatgt
    18901 ccaacgattc attatcaaat cgaacggtac cacagccatt gacacacagc aacacacgga
    18961 agctgattga atccgcacga tagtgtaccg tctgtcttct ctcagtgttg acaatcatac
    19021 gagagacctc gaaatgcttg catctgcaaa gcaattccgc agctacacca gcacgatatt
    19081 ttagaacacg aagcggctgt ttcggttctg acaacccttt caagttcgct acttccagcg
    19141 ctttgtctac atgaagttct ctctttttac catttttgtc aacacggtca taatcgtaga
    19201 gacgataagt cagattggaa ttttcctgaa tttctgcaat tagtgcaccc gcaccaatcg
    19261 catgaatcgt tcccgcctca ataaagaaca catcattctt tttgaccgga accttctgaa
    19321 gataacgctc aacagttccc tgagcaatcg cttcacggat cacagcttct tctgttattt
    19381 tctgcagccc atagattaag ctggcatcca gtacatacca catctctgtc tttccaagct
    19441 gcccgttttc tttttcaatt gcatattcat ctgtgggatg tacctgaaca gacaaatcct
    19501 ttttcgcatc aatgaatttg atcaggatcg gcaattctcc tttggtctgc ggatgcgttc
    19561 ccagaaactc tgggtgttct tgcaacacct ccgtcaaggt catcccctga tactctgacc
    19621 agacacaaca aagctcggtc catccgggtg cgtagagcat tccaagtttc cgcaagagga
    19681 gacatttcaa ttccttttgc aaattcatca ttcaggcggt tcccccccac agataatcct
    19741 ttgcggaagg cttcaataaa aacggtttct gtataacgag ccctcctccg ttttttaata
    19801 ctctaactca aatttctgtt tatctttaac attgatatcc ttgccaagct gtacttcaat
    19861 cagcttcagc tcagtatctg ccataatggt atgccgacag ccagcactca tggtgatcac
    19921 atcgccagcc ttaacaacct gctcatatcc atccacgatg gtcttaccag taccggccag
    19981 aaccacccac acctcgtcac ggttcttgtg gctgtgataa ttcatggagt gactagcatt
    20041 cagagtaacc ttaatggtca tgctctctgc ttctacatcc ataaccttat aactacccca
    20101 agacttctct gcgaacatga tctgctgctc gaacttgtca acgaaaggct taatatagct
    20161 ggactgttcc ttatcggaaa ccagaatacc ttcccgagat gcacagatga ccacatcatg
    20221 cagacccata gccaacacag gaacatccag ttcgttcagg atgtgtacgc catcgcaatt
    20281 ctcattcata acacctttac cgataatgga ttcctccata ccttcggtca gagtattcca
    20341 agtccccaga tccttccaca taccagcaaa acgctgtacc tgaatcttgt cctcatgctc
    20401 aacaacggca tagtcgaagg aaatcttagt cagagtatcg tatttgttga acagatcctg
    20461 ataatctaca aagtcaatca gctgatgtgc acgctccatg acatacttca gcttgtagac
    20521 aaacacacca ccgttccaca gtgcaccctg tgcgatatat tcctttgcca cagcctcaga
    20581 aggtttttcc ttgaaggtat ttacgaaagc agtctgctct gcgctctcag gaatgatgta
    20641 gccgtacttc tcggaaggat aagtcggttc aatacccatg agcaccagat tagcttcgcc
    20701 tttgtcagcc tgatcggaca gagatttcag ggcctcaaaa tagtcatctt ctacgtaagg
    20761 gtcaaccggg caaacaacta cggactcttc tggatcaatg cccatcacat ctaccagata
    20821 agcggtagcc aatgcgattg ccgggaaggt atcgcggcga caaggctcta cagatacacc
    20881 cacatcctca ccaagttggt tatggatagc agaaacctgt gtcttagagg ttgcaatcgt
    20941 aacagttgcg tcgttatcta ccttcttaat ctggcggtag acacgctgaa ccatggattc
    21001 atatccatct tcagttttga aaatcttaat gaactgcttg gaacggatgt cattagacag
    21061 cggccaaagg cgcttaccag aaccgccaga cagtaaaaca atattcataa tgatctctcc
    21121 tttttataag aaagaggctg ctgcaatcct caaaaatcac agcaacctcg gtaaattagc
    21181 gttcagcacg catcgggtta ttcaggaagt cctcgtagga cagacggatg ccatcttcca
    21241 actcagtctt atatgtccat cccagcttgg ttgccttgga aacatccagc agcttacgag
    21301 gtgtaccgtt cggcttgctg gtatcccaca gaatctcgcc ttcgtaacca ataacctttg
    21361 ctaccatctc ggtcagttcc ttgatagaca gttccttgcc agtacctgca ttaacagtct
    21421 cgtcaccgct atagttattc atcaggaaca cacacaggtt tgccagatcg tcaacatata
    21481 ggaattcgcg cagaggagaa ccatcacccc agcaggtaac agtaggcaga cctgcttcct
    21541 tagcctcatg gaagcgacga atcaaagcgg ggagcacatg actgtgagta ggatgatagt
    21601 tgtcgttagg tccatacagg ttagtaggca taacagagat atagtcagta ccgtactgct
    21661 tgtttagaaa ctcacagtac ttcaggccag aaatctttgc cagtgcataa gcctcgttgg
    21721 tcttttccag ctcacttgtc agcagacagc tttccggcat cggctgcggt gccatacgcg
    21781 gatagataca ggaagaaccg aggaactgta acttcttaca gccattctcc catgcggagt
    21841 tgatgacgtt catttccaga atcatgtttt catacatgaa gtctgccaga gcgttctggt
    21901 ttgcgataat accgccaacc tttgcagcag cgagaaatac ataatccggc ttttcctgtg
    21961 cgaaaaatgc ttcaacagcg tcctgacggc acaggtcaag ctccttatgg gtgcgggtga
    22021 taatattgtt gtagtcctga cggttcagct cgcgcacgat tgcggaacca gccatgccac
    22081 gatgaccggc tacgtagatt tttgcgtctt tttccatcat aataatgctt gctcctttct
    22141 aaatttagag catctctcat tgcatgagag aaatcaattt gtctgcgatt tcgctcgcag
    22201 catcataaaa tgtatcaaaa tttgaggtgt ctagctgttt gaggtcctca atcacccgct
    22261 ctacgcggtg gaggttttcc ggtgtgacgg aaattgccag acccagtttt tgcaatttac
    22321 aaatagttgt tctatcttcc aaaactgtat cgcgggcgaa cagtgccatc ggacgctgtg
    22381 cgagaataca ctccgcaaca gttccccatc cagcctttgt aataacgtaa tctgccgccg
    22441 ctatatagtc ctgtgtattg ggggtgctgt tagaaataac ggttacgttt tctcccacaa
    22501 gcggaacacc tagcgttgta atgaaatcat acggcgcgtg actgacatca atcggctgtt
    22561 tgaaccgcgc ggacataccg agtgcgacaa atacaggcgg tttttcatgc tgtgatttaa
    22621 tgcgttgcac ttcgtcgtta tgaatcggac gcgccacaac ggaagtcggt gttttttcgc
    22681 tgttttggag aaattccaac atttcttcgt tatttagcgc ataaagcatg gtgtgctgaa
    22741 ttttaccgta acgcttggca tattctttcc aaatatgttc gggtaaaaat tcgcgataca
    22801 gctctgtcca agtgaagttt cctacataca ggaaaggtac tcctgccata tcgcaggcat
    22861 ccattgccca gattggcata tcgcaaagca ctgcgctgac agcttctcgc ttgagccatt
    22921 cggcttctgt ctttgccaaa gaaggaacct ttgccaaaaa atattcacag atctcggtca
    22981 gcttacccgt gtccaccagt agggtgccct cacggttaat cagaccgata tctgtgttta
    23041 ttggacgata gcgcacgcgg tctatcagct ctttgggcag ttcctgctcc aaattgcgct
    23101 ttgcaaaatc cagatgattt gcgccgcaaa ccagtgtaat cttcgcttcg catcggcaga
    23161 gtgttgccat aataatgggc agattccgcg cgatatgtcc gaagccgtga ctggaaagat
    23221 agaaacagat atgtttattc aatgtcagct tacttgactg cgttcttcat tgcagcttcg
    23281 cgctttgcca attcacggtc atgctttgcc atgatgttaa ccagttcctc gtaagaggtc
    23341 ttctgcggat tccaaccgag aacggtcttt gccttagtcg ggtcgcccca caggttgtca
    23401 acgtcagtcg gacggaacca ctgtggattt acacaaacga gcatcttgcc ggtttcaaca
    23461 tcgtagccct tttcatcaat gccagtgcct tcccaacgaa tggtcatgcc atttgctgcg
    23521 aatgccttct cggtgaagtc acgaacagtg tgctgctcgc cggtagcgat cacgaagtcc
    23581 tcgggagtct catgctggag aatcatccac atgcactcaa catagtcctt tgcatagccc
    23641 cagtcacgca gggagtccat gttgcccagt tccagatggt cctgcaggcc ttctgcaatg
    23701 cggccggcag cgagggtgat cttacgggtg acgaaattct caccgcgacg ttcggattca
    23761 tggttgaaca gaataccgtt gacagcgaac atgccataag cttcacgata ttccttagtg
    23821 atccagaagc catactgctt tgcaacagca taagggctgt aaggatgaaa cggagtggtt
    23881 tcacgctgag gaacttcttc aaccttgcca tacagctcag aagtagatgc ctgataaatc
    23941 ttagtcttct tcgtcaggcc cagaatacgg caagcttcca gaatacgcat aacgcccaaa
    24001 gcatcaacat cgccggagta ctcaggaaca tcaaaagaaa cctgaacatg gctctgtgca
    24061 gcgaggttat agatttcatc aggctgaaca atgctgatga tacgaatcag agaagatgag
    24121 tcggacaggt caccgtcatg aagggtgatc ttgcccatca aatggtcaat gcgagcggta
    24181 ttggaaatag aagcacggcg agtgataccg tgaacttcgt agcccttctc tagcaggaaa
    24241 tctgccagat aggaaccgtc ctgaccagta ataccagtaa tcagcgcttt tttcatgata
    24301 cattatcctt tcctatgtaa gtgataaggc catataaatg gctcgtaatt tctaatactt
    24361 ttcaagaata cacgataatt ttcgatattt catagaacaa tctacgcacg cattagcaca
    24421 atattagttt ttcgtgaatt ggaatggcaa cagttttttt gacaaatttt ataaggtgca
    24481 gaacttcttt ccgtatgcta ttccgattgt ccttgacaat gagcctcctc ggatgtgacg
    24541 atcttcggca ggagctaaca gttaaagtta gactgcctcg ctagcgttag cacatccgag
    24601 ctcattatca aggattcgct gcgccaatct ctggttacgg taaccaaccg gtgtctcgta
    24661 gccaagtgta ccgtgcaacc gaaggcggtt ccaccaattg acatagtcaa ataactccaa
    24721 atccaattgt tgtaaggttt caaatgtgta ttgatagaca aattctactt tcaacgactt
    24781 ataagttgat tcagctacgg cattatcaaa aggacagcct ttatgactca atgatcgatt
    24841 gatgtcaaaa gttgttgata attcatcaat agcttggtta tcaaactctt ttccacgatc
    24901 agtatgaaaa atctcaacct ctgtcagagg tt
//
LOCUS       DAPCMU010000026        24474 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPCMU010000026 DAPCMU010000000
VERSION     DAPCMU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.026"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(45..893)
                     /locus_tag="RD744_000888"
     CDS             complement(45..893)
                     /locus_tag="RD744_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0556908.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1117..3057)
                     /gene="thrS"
                     /locus_tag="RD744_000889"
     CDS             complement(1117..3057)
                     /gene="thrS"
                     /locus_tag="RD744_000889"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDU0556909.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3394..3573)
                     /locus_tag="RD744_000890"
     CDS             complement(3394..3573)
                     /locus_tag="RD744_000890"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDU0556910.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3620..5443)
                     /gene="cadA"
                     /locus_tag="RD744_000891"
     CDS             complement(3620..5443)
                     /gene="cadA"
                     /locus_tag="RD744_000891"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDU0556911.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5721..8000
                     /locus_tag="RD744_000892"
     CDS             5721..8000
                     /locus_tag="RD744_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0556912.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8093..8740)
                     /locus_tag="RD744_000893"
     CDS             complement(8093..8740)
                     /locus_tag="RD744_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556913.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8919..9638)
                     /locus_tag="RD744_000894"
     CDS             complement(8919..9638)
                     /locus_tag="RD744_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0556914.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9769..10920)
                     /locus_tag="RD744_000895"
     CDS             complement(9769..10920)
                     /locus_tag="RD744_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDU0556915.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11137..11877)
                     /locus_tag="RD744_000896"
     CDS             complement(11137..11877)
                     /locus_tag="RD744_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0556916.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11881..12204)
                     /gene="rsfS"
                     /locus_tag="RD744_000897"
     CDS             complement(11881..12204)
                     /gene="rsfS"
                     /locus_tag="RD744_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDU0556917.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12261..12854)
                     /gene="yqeK"
                     /locus_tag="RD744_000898"
     CDS             complement(12261..12854)
                     /gene="yqeK"
                     /locus_tag="RD744_000898"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDU0556918.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12838..13488)
                     /locus_tag="RD744_000899"
     CDS             complement(12838..13488)
                     /locus_tag="RD744_000899"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDU0556919.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13498..13809)
                     /gene="yhbY"
                     /locus_tag="RD744_000900"
     CDS             complement(13498..13809)
                     /gene="yhbY"
                     /locus_tag="RD744_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDU0556920.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13825..14934)
                     /gene="yqeH"
                     /locus_tag="RD744_000901"
     CDS             complement(13825..14934)
                     /gene="yqeH"
                     /locus_tag="RD744_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDU0556921.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14931..15458)
                     /locus_tag="RD744_000902"
     CDS             complement(14931..15458)
                     /locus_tag="RD744_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDU0556922.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15638..16423)
                     /locus_tag="RD744_000903"
     CDS             complement(15638..16423)
                     /locus_tag="RD744_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDU0556923.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16416..17285)
                     /locus_tag="RD744_000904"
     CDS             complement(16416..17285)
                     /locus_tag="RD744_000904"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDU0556924.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17286..18665)
                     /locus_tag="RD744_000905"
     CDS             complement(17286..18665)
                     /locus_tag="RD744_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDU0556925.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18673..19092)
                     /gene="fabZ"
                     /locus_tag="RD744_000906"
     CDS             complement(18673..19092)
                     /gene="fabZ"
                     /locus_tag="RD744_000906"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDU0556926.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19117..19593)
                     /gene="accB"
                     /locus_tag="RD744_000907"
     CDS             complement(19117..19593)
                     /gene="accB"
                     /locus_tag="RD744_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDU0556927.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19596..20831)
                     /gene="fabF"
                     /locus_tag="RD744_000908"
     CDS             complement(19596..20831)
                     /gene="fabF"
                     /locus_tag="RD744_000908"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDU0556928.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20850..21587)
                     /gene="fabG"
                     /locus_tag="RD744_000909"
     CDS             complement(20850..21587)
                     /gene="fabG"
                     /locus_tag="RD744_000909"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDU0556929.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21599..22516)
                     /gene="fabD"
                     /locus_tag="RD744_000910"
     CDS             complement(21599..22516)
                     /gene="fabD"
                     /locus_tag="RD744_000910"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDU0556930.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22623..22847)
                     /locus_tag="RD744_000911"
     CDS             complement(22623..22847)
                     /locus_tag="RD744_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDU0556931.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22900..23862)
                     /locus_tag="RD744_000912"
     CDS             complement(22900..23862)
                     /locus_tag="RD744_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDU0556932.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23872..24312)
                     /locus_tag="RD744_000913"
     CDS             complement(23872..24312)
                     /locus_tag="RD744_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0556933.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6703 a         5441 c         4254 g         8076 t
ORIGIN      
        1 cgttttgtta ttctaaaaaa cttatataaa aaatagtaag gtttttattt cgaatccata
       61 aaatattctt ggattgcttc tcgtgttttt ttcacttttt cgctaagctt ttcgttttta
      121 gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag tgaattagtt
      181 gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac atcggctatc
      241 ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt catgcttttc
      301 gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac aagccaaagc
      361 actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc tttttcaaaa
      421 atgattggct taccgacacg agaccatttt tgcagaatat cttctactac taaagaagag
      481 gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac gactttttgg
      541 atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc attgttacca
      601 agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa accttcatcg
      661 attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg
      721 atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc tgctaattca
      781 ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc caactttttt
      841 tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct cataagttcc
      901 tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttat tcacggtcgt
      961 atactggata aaaaacaaaa aaacaatccg cccaacagaa tatcttatcc catcaatccc
     1021 acagattcta ttgagcatct gtgtctttga ggaatacctg ttatctattg ttcggattgc
     1081 tttctatcta ctactttttt acaggaaatt tacctattag ttttctctgc tgtaattttt
     1141 gacttctgct tccatagcat caataaagat attcaaatct tctacagctg tttctttact
     1201 tccataacga cgtacgttca ctgttgcatc ttccaattcc ttgtctccaa caactaattg
     1261 gtaagggatc ttttgtgttt gagaggcacg tatcttatag cccatttttt cattacgatc
     1321 atcaacttct acacgcaacc ccttcatttg caaacgttct ttgatttcat aagcataatc
     1381 gccatgtgca tcaacagaaa cagggatgat tgttgcttgt ataggtgcca accatgttgg
     1441 aaaagcgcct ttgtatactt ctgttaaata agcgacaaaa cgttccattg tagaaacaat
     1501 tccgcggtgg ataactactg gacgatgcgt attttccccg tcttctccaa catatgtcaa
     1561 atcaaagcgc tctggcaata agaagtctaa ttgtatagtt gataatgttt cttctgtacc
     1621 aagagctgtt ttgacttgaa catctagttt tggtccgtaa aaggctgcct cgccttctgc
     1681 ttcaaagtaa tctagttcta attcatccat agcggctttc agcattgctt gggctttttc
     1741 ccacattgcg tcatcatcaa aatatttatc tgtattgttt ggatcgcgat agctcaagcg
     1801 gaaacgataa tctgtaatat tgaagtcagc atatactgcc accatcaatt caagcgtacg
     1861 tttaaattca tctttgattt gatcaggacg aacaaatgta tgaccatcat tcaacgtcat
     1921 ttctcgtaca cgttgtaaac ctgacaaagc acctgatttt tcatagcgat gcatcattcc
     1981 taattcagcg atacgaattg gtaattcacg ataagaatgg atcgtgtttt tgtagaccat
     2041 catgtgatga gggcagttca ttgggcgaag aacaagcatt tcgccatctc ccatatccat
     2101 tggcgggaac atgtcttctt gataatgatc ccagtgtcca gatgttttgt acaactcaac
     2161 atctcccatt attggtgtat atacatgttg gtaacctaga ctaacttctt tgtctacgat
     2221 ataacgttcg atcgtgcgtc ggattgttgc cccttttgga agccagaatg gcaaaccaga
     2281 acctacttct ggggaaacca tgaacaagtc aagttctttt cctagtttac gatggtctct
     2341 ttcttttgct tcttcacgga gacggatata ttctttcaaa gctttcttgt caaaaaaggc
     2401 tgtcccgtat acacgttgca tcatttgatt gtttgagttt ccacgccaat aagcacctgc
     2461 cactgataat aatttaaata cttgtatacg gccagtggat ggaacatgtg gtccacgaca
     2521 caaatcgatg aattcgcctt gttgataagc agtaatcact tcttcttctg gcaattcact
     2581 aatcagttct actttgtacg gatcacttgc aaaaatttct aatgcttcct gttttgaaag
     2641 aacacggcgt tcgatcggca gattttcttt gacgattttc atcatttctg cttcgattgc
     2701 cggcaaatct tctgcagtta cttggttttg tccattatct gtatcgtagt aaaaccctga
     2761 atcgatagct ggtccaacgc caaagtggat atttggatag aggcgacgca tggcttgggc
     2821 catcaaatga gctgctgaat gacggactaa gccaagtgca tcttcatggt ctggtgtgat
     2881 gatctcaata cttgcatctt cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac
     2941 tttaccagct aaagcttttt ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt
     3001 tgttgttcca gactcaaatt ctttgactgc accatctgga aaagtaattt ttatcataat
     3061 agtttcctcc ttgaaatgtt ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc
     3121 tagcatctct tgaaaagaga tactaggacg aaaagttcgt ggttccacct aatgttcaaa
     3181 cgcaaatgcg ttcctcaagc gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc
     3241 gaaagtggtc atcctttggt tttctcctgg aaaagtttca gcctagcttt tcctctctta
     3301 tcgaagactc tcaaattcag ttggctttct catcaatcaa ttatgtgttt atttaaccac
     3361 taataaatag aaaaatcaag tgggaaacta aacttatttt ttccattccc catttacacc
     3421 gtatgttcct tttttcatct cattgatgat gacatggatg tgttcttttg gtgcctgcgt
     3481 gtttttatga acagcttccg tgatatcttt catcatattt tctaattgtt ccggactgcg
     3541 accttctact aattcgacat gtacgaatgg cataatgctc tctcctcttt ctgatcgttc
     3601 ttttttcaac tcgattctat catactatgt ttgtcttggg tactgaatca ggaaaaatgg
     3661 ctggatcttt ttttgctctc aaggcactca aaatagagac cgtatccacg acttcttgca
     3721 aaacagctcc gaataatgcg gggattagtc ccgtacttgc aattaacatc aaaaaaatac
     3781 aaataaagat gccaatcagt acagactgtt tcgctattct catcgtatct cttgaaatca
     3841 ctactgcttt tgctacacgc aagagatcat ctttcaagat gaccgcatct gcactttcac
     3901 tagcagctgt agaaccatga gcccccattg caataccgat atctgcagca gcaagtgctg
     3961 gtgcgtcatt cacgccgtct cctaccatcg caacaggtct ttgatcttta gggatacttt
     4021 ctaaaatggt cagtttatct tgaggcaagc tacgggcatg tacagaatct aattggactt
     4081 tttctgcaat cgtttgagca atggcagatc catccccggt gatcatcatc aaatttttca
     4141 catcaagttt tcttagctcc ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa
     4201 ataggatcgt tcctgcaaaa gatcgatcaa ttgttacata gattgctgta ttggacgttt
     4261 ctatttcttc agctgaaaca aaagaggcac gtccaatctt tacttcatga ttattgacta
     4321 ctgcttggat tccttgaccg gtcacttctt tcagttgact aacaggatac agttggctgt
     4381 ttttcacgtt gttcactaac gaacgagcta agatatggct agattcctgt tctgcactag
     4441 ctgcatacag aagtagctct tcttttttta ttccattgac tggctgaatc gcctctactt
     4501 ccaatatccc tttggtaagc gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga
     4561 gcttttcaat cgttgttcca gtttttacaa cgatcccgtt tttacttgaa cggctcattc
     4621 ctgcaactaa agcaatgggc gcagctagaa tcagtggaca aggtgatgcc actaccagta
     4681 cctcagcaaa tcgtaaaggg tcttttgaga accaccaaga aataccaccg atcaaataag
     4741 caatcaacgt gaaagggact gcataacgat cagctaaacg gacaaatcgg gcaggctttt
     4801 cttttgattc ttcgactagt ttaataatca tttgatactg gctgtctgcg gcacgttttt
     4861 cagcgatcat ttggatcgat ccgtctccat ttaaagaccc tgacataatc tgatctccag
     4921 gtttcttggt ttccgggcgg gactcacctg tcaaagaaga ttcatcgacc gaagattctc
     4981 ctttggtcac tcgaccatct gcgggtacaa gttcgccagg cttgactagt aactggtcat
     5041 caatatttat ttcatcgatt actatatcct gcaattttcc ttctttgaat cgatgggcaa
     5101 ttcttggggt gttatctaac aaagactgca gttctttacc cgctcgttta ttcgcgtaat
     5161 cctctagact atctccgccc gtaagcatga tcaatatcat caaactagcc cagtattctt
     5221 tgacggctaa tgttgcaagg attgcggtaa tcgctaatac atctacgcca tatcttcctg
     5281 aacgtaacgt cttgatcatt tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg
     5341 cgacaatcgc aaatgcaagg gccggctggt gaaaaacaaa ttcacttaat aaagctatcc
     5401 caccaataag caaagtaagt aaaaattttt tgaaatgtgt cattgttttt tctcctcttt
     5461 tatgtgactt agagaggtta gtaataaatt tccagctgcc tctttcatct taagcatagt
     5521 ccttgactag cactttttaa aacaataggc cttaattgaa aacaactatc actatcagcg
     5581 tttccaaaat tcatcagttc tacaaacaaa aatattcagc aaggattact ttttcgattc
     5641 atcagttaaa aagtaccttt tttcgaaaaa ctatgctata cttttaattc gttatttacg
     5701 gcggaaggag caaacatgcg atgaatgaga aacaactaga acaaacgcac gtggatgaaa
     5761 cgatcgaact aatccagatc gaacaaaata tgctggatac aaaacaacaa gaattgactc
     5821 aggaaatgaa tcattcgaca aaagacacag ctaatcacaa gattcgagct ggttccaatg
     5881 aagcttttta tgaatctgct gttgaatatc gccaacatga gcaggaattg cttttgaaat
     5941 accatactct tgaagcacag caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc
     6001 cttattttgc ccgtatcgac tttaaagaag accgtgaaac agaaacatta tatctaggta
     6061 tcgcttcttt aagagatctg gcagaagaaa caatcgttat tgactggcgc gcaccaattg
     6121 ccaatcttta ttatgaaggt gagattggtc ccgcatttta tgaaactgaa acagatagaa
     6181 tagatgtcga tctgcagttg aaacgacaat ttaaaatcca agaaggacgt attttatcta
     6241 tggtagatac gtcggaagta atcaatgatg actttttgct agagatattg gatgaatcat
     6301 caagcaacca tatgaaaaac atcgtctcta cgatacaaaa agcacaaaat gcaatcattc
     6361 gggacacaac aagtaaagta atgctgattg aaggaatcgc cggaagtgga aagacatccg
     6421 ctttgcttca acgtattgct tttctgttgt atcataatcg caagtggctc gaggctgaaa
     6481 acgttcttct attttcaccc aaccatttgt tttctgatta catctcgact gtattgcctt
     6541 cattaggtga aagcggcgtg cctacacaga cattcaaaaa ttatttggtg cagctgctgc
     6601 cagagttttc tttactagat gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag
     6661 atccgataca agtgatgaaa agcggattga ccttagtcga tcagattgat cgctatatcc
     6721 aaggcatcac aaactacggt cccttgttcc gtgatatgaa aataaatggc cgtacgatcc
     6781 tatcaaagga atcgattcgc caatggtata aagaaacaaa tgaacaatta cctttacatc
     6841 atagactttc tctattacaa acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg
     6901 aaacacgtca gcaatgggtc aaagatctgg cggaagaaca gctccaagaa ctatacgcaa
     6961 cagatccaaa tcttgaatat acagaacaaa aggagcgttc attacgtaaa aaactggcca
     7021 accaaatcgt acgtaaaaga ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg
     7081 tcaatataac gaaacaatat ctccactttc tacaaactgt gccactttct cttctaacac
     7141 gacataaaat cagcgagacc aggtggcagg aacatttgac aactgttcgt acacacttgc
     7201 gtcaaagaga attgcgtcaa gaggatgctg tactattctt tttactgatg cgccgaacat
     7261 atccagtgac ggttacacac aaagctcgct atatctttat tgatgaaatg caggatttcg
     7321 caccatcaca agtggcattg ctgcaagcct tgttcccaaa agcaaatttg actttctgtg
     7381 gagatctaaa tcaaaatgtc tttggaaatg aaacgatcgt tggatcattg gaagaccttt
     7441 tcgtggaccg tgaagtcact cgtttccaat taacgacaag ctatcgctcc acaaaagaaa
     7501 tcactgattt tgctaatcat tttattgtga aagaacatca agtagaaaca acagcaagaa
     7561 aaggagacct tcctttgctc atccaaggaa caacagatga tatcaaattg aattggttag
     7621 cagataccat tcgaacgact cagggaaaag cccggtattg gcgtacagca atcatagcta
     7681 aaacacaaga agaatgcgaa gctctttatg ctcaactgcc agacgagttg aaacaacaag
     7741 tccagctgat tttaaatgag agtgacttta tgaagcgttc cattatttta cttccagcct
     7801 ttttagcaaa aggtctggag tttgaccgag ttttcttgtg ggatgtagat gcagaacatt
     7861 tttcttctca gcaagacaaa ttgatccttt acaccatgtg tacacgtgcg atgcatgaat
     7921 tgaccctttt tacctccaat aatgaaagtc ctttgttaaa caatatggat atttcatctt
     7981 atgagaaaaa agtactgtaa gtaaaaagac aactgctatc attaatgtta gcagttgtct
     8041 ttttatttac aataataatt gcagaaaaag taattcaatc atttaaggtc atttattcta
     8101 tatataaatc caaatctgtt tcaggatatt ttaatttgac aagcgaaata atcattttca
     8161 gttcttccat gttttctgga tattctgggt caaatacctt gctatgacgg ccaatcccgc
     8221 cattgtcgat caatcggtaa attcgattcc attcaatgaa ttggtcaatt gattggaaat
     8281 tgctgattgg atagccgcca caatccaaaa tctctgatac acgaaatgct aggtatgcac
     8341 tatcgatcac tttatttttt ttcgtgtcta tcttctcatt tctttcgata agaatttttt
     8401 caggacgatg tttcaagctc cactctaata gtagaaagaa agcctccatc cgatactctt
     8461 cttcaaataa ccgaaaggct gaatcagaac gacgaaaaag cagcggaatc agataatttt
     8521 gtctataaat cagcattggt gcgcggtcca ccatacactc taaataacgg ttcagatacc
     8581 ctgtaccgac atagtttcct aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg
     8641 ctaattttga acaatcagtt acgttcacag ctaactcttc tactttgcgg taaccgctta
     8701 agatacgttc aaaaggttct aaagtgattt ttgcttccat attcttcccc gtccatcttc
     8761 cacatacttc atatcatttc acatgatact tatgctctca tcataccaca ggttgaatta
     8821 gaaaagaaaa taaatcacac aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt
     8881 tcgaaaaaga ctgaatactt ttcgttaaaa ctggttgctt agtcttctaa tgccacatta
     8941 tggaacacat gctgtacatc ttctagatct tccagtgcat caatcatttt ttcaaatgtt
     9001 tcttgatctt ctggagatag tgttacctcg ttttgaggga tcatttggat ctcagcgatt
     9061 gaaaaatcag tgattccttt ttcttttaat gcttcttgta tactatggaa atccgctact
     9121 tcaccgtaaa cgatgatttg tccttcttct tccattacat cgcgaacatc gatgtctttt
     9181 tccattaggt attcaaggat ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta
     9241 ttgtcaaaca tgtaagaaac cgctccggaa acacccatgt tcccgccatt tttcccaaat
     9301 gctgcccgta cgtctgctgc tgttcgattc acattgttcg tcaatgtatc aacaatcacc
     9361 attgagccgc ttggtccaaa tccttcatag cgcaattcag agtatgtttc atctcctgca
     9421 ccttttgctt tttcgattgc tcggtcaata atatgttttg gtacattata agttttagcg
     9481 cgatcaatga cgaaacgtag tttttggttt gcatgcggat caggatcacc tgattttgct
     9541 gccgcgtaga tttcaatccc aaattttgca taaatccggc tattattagc atctttagca
     9601 gttttctttg ctacgatatt tgcccattta cgtcccatat tttcacttcg ctttctgatt
     9661 tgatagtttc ataacgcaaa aacgtcctgt tccattatac ttgtgaatca cgattctgtg
     9721 aagctttttt tctttcttcc ttcattcttc tcatttttag tcaattatct atactcggat
     9781 tgggatttgc ccgaaattct gttccggaat attgcctccc aattgataga tttgatcact
     9841 tctaactaac agttccgctt cttgtgcctc tttttttccg attcgggaat aaatagaaat
     9901 ctcttttttt tcttttaaga atcttcttcc agcatcggta aatccaagta gtcgaagata
     9961 atttttattt tgttggacag gaatctcttg ctttttgata ttcagcaaga cataacaaga
    10021 taatcgttgg atccgtgtcc atgtatatct ttttgttttt acctttgaca caaaatcctc
    10081 aaaactatca gcagttgcag ctgctgtttt catgcgcgat tctattcctt cggtcatctg
    10141 atagatatct gtcagttcca gcaaagtaga agataacaac cgataacgca aatacggcca
    10201 ataagattcc caagtctgca gcgaacaagt tgaaagatct tcttctgtct gaggcggcac
    10261 aagtccagct attctttttc cttcttctaa cgcatggcga atggctgttg cactggctat
    10321 cctctgatct gtgatgattt tttcatgata gcctgcactt ttacgtcgga tcggatgtaa
    10381 ttccataggg tggacatact tggcattttc ctttgcataa cttaatccca agatatgatt
    10441 tggcgaagaa aaatcaagcg taatatcggg atacagactg cgaaaaacct cagtcatttt
    10501 ttgcggataa ctatacgttt gatcagacag ttcttgaaat gcctcttcta tcctatcttg
    10561 attctcttgg acaaagcgtc cgaaactgct gtaatcaaac gaagtctctt cttctgttcc
    10621 aaaacacaaa ctgtcacagt ccagtgtttg caagattttt actgcacctt tagcaaaata
    10681 gtcagctgac tgaaccgccc attcgacggg aagctccact actaaatcca caccattttg
    10741 taaagcttca ttcgcgcgat gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa
    10801 attcccgctc attacagcaa tgacgacctc tgctcctgtt atttttcgtg ccatttccac
    10861 atgatagcga tgtccattgt gaaaaggatt gtactctacg ataatcccgc agcttttcat
    10921 gcgcatcctc cttcatagtt atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa
    10981 aatcggaaaa aatacccgaa ctatataaac tattctcgca aacacaatac ttctgcgacg
    11041 aaaagctgca ctgcaagagc aatgcctata tcgttcggat atataattta tgttgaagat
    11101 ttattctttt cttgttaact cgagcaggca aaaccgctag tctttgtatg ctacaaagaa
    11161 ccagcgcttt gatttctctg atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt
    11221 cgaaaagccg ctgttttcca gcattcttaa ataactttcc aatggatagg tacgctcttg
    11281 atgtagttca tcttcacgaa taaatcgacc agacccttct tgttcttcaa cgaaaaaggt
    11341 aagaaaatgt tcgatgctgt gtttctcgct tcctggataa ctatcccaca aaaaagcaaa
    11401 attatcccct tgataatgat agctatattc tggaaaaacc tcatctactt gataggtgga
    11461 gtgtacgtca aataaaaatc ggccattttc ttctaacgct tgatagactt catcaaacac
    11521 ttgctgtacc tcttgtctat ttttcatata gcacagcgaa tcagaaaaac aggtaattgc
    11581 ctgatattgt ccgatttctg ataaatcgag catattgcct tccacaaatt ggatcgatgc
    11641 gtcttcttca tatgctcgct ggctggcaat catcaacatc tcttctgaaa ggtctaaccc
    11701 tgtcacttta tatccatctt ttgcaaaggc aaccgctaat gctccagtac cgcatgccag
    11761 ttccaaaata ttttttgtgt cttcttgtaa atgacgtttc gaaaaagcta accattgctg
    11821 ataaagtgtt tcgtccatca cttcatcgta aatgaaagca aatgtttcat aagccatatg
    11881 ttaatccaac cattcactaa gatcgactag cggagcatct gaccatagtt tttccagatt
    11941 atagaaattt cgttctggcg catggaaaac atgtacgatc acatctccaa gatcgatcaa
    12001 tacccatttt ccgccttctt ttccttcaat acgttttact tcatatttgt tttcttcttc
    12061 tttatcaatg atttcttctg taattgcgtt gatttgacgt tcgctatttg ctgaacagat
    12121 catgaaataa tctgccaaca aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc
    12181 ccgttttgaa tctgctgctt caatcgcaat ttttaattgt tctttactat ctatgatgat
    12241 ttcctcctaa tatgagcagc ttattttttt gctacccagt aattatatgt ttcgattgtt
    12301 tttggataaa tctgttgttc ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat
    12361 gctacagctt cgtctagatc agcccatgca atggctcgtg catcttgtac cccaggaaaa
    12421 tctcgacctg gttcaatata gtctgctaca taaatgattt tgtcaaggag actcatctta
    12481 gcagccccag tcgtatgcac ccggattgca tgaagaatct ctgcatcggt gattcctaat
    12541 tcccgctcta caaacgaagc gccaacaaga ccatgccaga ttgcattatt ataattcaat
    12601 aactccggat caaaaccgtc tcttacgatt accattttga actcctcatc tggtcgttct
    12661 tttgcataat catgtgtcaa tgcagcaata ctagcttttt caggagaagc accgtatttt
    12721 ttggcaagag ccacggctgt ttcttctact ccaagcacat gcttgaagcg ttgttcgctc
    12781 attctcatct gtattttttg catcaggttt tctctagaat aagcagtata ttcattacta
    12841 tagtccatat tcatacaacc cttcattttg aatataatca attactttat ctggaacaag
    12901 gtagcggata gaacagcctt ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat
    12961 ctcaggtaca tctacccaaa ttacaggata tggagtatcc gttgtgtatc ctggtcgacg
    13021 tatacccaca aagttgacca tagaagtcag ctcatcgatt ttataccatt ttggcaaata
    13081 ttctaccata tccccaccga taataaagta ataatctgta tccggattgt tttgcgtcaa
    13141 ttccttcata gtatcatagg tgtaactttt cccaccacga gctaattcaa tcgtttcaat
    13201 ctgcaaaaaa ggattgtctt ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc
    13261 aatcgtctgt ttctcatcaa catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc
    13321 aagacctaaa ttccggccag cttgttcagc catcactaaa tgtgcaaggt gaacgggatt
    13381 gaatgttccg cccaagatcc ctacttgctt tttcttctct aagaattgag gcatttcttc
    13441 aggaaataat tgtgaacgaa caaatgtctt agcttgcgta ttcatcatgg tcactcctta
    13501 tattgctttt acttcttttg atagcctttg atacttttct tttgtggaag gtttgtataa
    13561 tacaataaca cgtccaatga tttggacgat ttcgcaatct atttcttttt ctaatacttg
    13621 agctgcttct tctgcaatct catctgtatt ttgaagtaac gatactttga tcagttctct
    13681 tttttctaaa gcttcttcaa tttgaacgat cattgctgaa ttgatcccac cttttccgat
    13741 ttgaaaaatc ggctgaagat gatgcgcttt gctacgtaaa aaacgctttt gttttcctct
    13801 taaactcata attttccttc ttccttaaat caatgctttg cgtttcaata catctacacc
    13861 ctttggcgcc cagcccgcaa ttactactgg cgctgttaca gtgatccagc ctagaccggc
    13921 aaaaacgatg tctgtttttt ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc
    13981 ctcgacttca tcaggacgag gaggctgtaa taatccacct acatgttttt gataaaattc
    14041 gtctgctttt tccagtttcg ttcgatgaat atttacatca tttgacacat aagcaataaa
    14101 tgaagcacga ttgccgctga taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg
    14161 cccttcattc agctgataaa ctttaggttt gatttctttt aacggtgcaa tgatccgcaa
    14221 atctttttta cctaagtagt gcgccatttg atggcgatgg ataattcccg gtgtatcaat
    14281 caaaaaatgc ccgtcatcta aaggaatctc gattttatcc aaggtcgttc ctggaaactg
    14341 cgaagttgtg attacatctt gtactcccgc tgtatttttg atgatttgat tgataagcgt
    14401 cgattttccg acatttgtta ctccgacgat atagacatcc cgtccatcgc ggtattcttc
    14461 gattttttct aatagttccg tcatctcatg agatttctta gcactagtta ataatacatc
    14521 tactggacga attcctgctt cgtaagcacg ttccttcatc cattgtgtta atttagtttt
    14581 tttcaatgat ttaggtaata tatccacttt gttcccaact aaaagtactg gattgtcacc
    14641 gacaaaacgg tgtagtcctg ggattagtga accattgaag tcaaaaatat ccaccacatt
    14701 gacgatcaat gcatcttttt gccctaattc attcagtaag cgcaaaaagt cgtcatccgt
    14761 caatgagaca tcttgggtct cattataatg gcgtaatctg aaacaacgct gacaatagac
    14821 ttccccggaa gctaaacctt tttccaaagc cgtttgcggc gtgtatccta attcaccggg
    14881 tttgtttgtt tgtataacgg caccacagcc gatacattgt aattcttctg tcattcgatt
    14941 ccacctcgcc agcccatatc agggtgtttt gctaatagat aggccataat tcttttttcc
    15001 caaaaacgat taaattgtgt cttccaacta tctgttgcga taatgggttt tacaaggatg
    15061 ctacgtatcc ctgcacgatt ggctccacgt atatcagtca taatctgatc gcctaccatt
    15121 actatctcat catctgctaa atccaattgt ttctttgcta tgttgatccc tcttgctaaa
    15181 ggtttcattg ctcgtgcaac atacaaaaga tcaaattttt caatcgctcg cgcgacacgt
    15241 tttgaattat tgtttgaaac aacaacaaca gggattcctg catttttcat ttctaaaatc
    15301 caagtaagca actcttctgt tccatctgga ttattccatg caatcaacgt attgtctaaa
    15361 tcggtcaata ctgctttgat gcctagattc tttaattggg caggtgtaat ctgatagatg
    15421 gcttctacca tccatgttgg tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa
    15481 atgaaggagc acataaagta ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa
    15541 ctacctctat actcgttcgt ctttcagaaa aagcatttct ttacaaaatg accaaaaata
    15601 aagattgatc acagaaaaat ttagaaacaa aaaacaacta atattggcgg aacctcttgt
    15661 atcgtttttc cagtaattta tgctgatcta atcgaacaag ttccgtcaat ctttcaatca
    15721 acgctttttt cataatgtgt gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt
    15781 caggaataac tcgatcaacg attcctaact cgcgtagttc tggcgcagta attttcatca
    15841 actcagctgc ttctgcggcg cgactaccgt ctttccacaa aatcgacgca aatccttctg
    15901 gagaaagcac agcataaatc gtttcttcca gcatccagac ttcatccgct aaacctagag
    15961 caagggcacc gccacttcca ccttcaccaa tgatgatggc gatgatcggt acctttaaat
    16021 cagacatttc gagcagattt ctggcaatag cctctccttc gccgcgctct tctgctccta
    16081 caccgcaata tgcacctgct gtattgatca aggtaatcac cggtctattg aacttttccg
    16141 cttgtttcat cagtcgtaaa gcttttcgat agccttcagg atggggagaa ccaaaatttc
    16201 tgcggatatt ttcctgcaga tcccgtcctt tttgaatacc gatcactgtt accggtttgc
    16261 catctaatcg agcgattcca ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat
    16321 ggcattcctc gaaagaatcg aagatctctt caaaaaaatc caagcttgtc catcgttctt
    16381 tagctcttgc taactgaacg acatcatgtg ctgtcttatc cacgatctat ccatcctttc
    16441 tgtgtatgca attcaagaag cgtatggatt ttctgtttca attccatgcg gggaacaatc
    16501 tgatcgacaa aaccatgaga taataagaat tcagcctttt ggaaatcttc tggaagttcc
    16561 tgcttgatcg tctgttcgat gacgcgtctt ccagcaaaac cgatcaagct ttggggttct
    16621 gctagaataa tatcgccttc cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc
    16681 gttaaaacag ttagataaaa aagaccttca ttgctatgcc gtttgacagc agcagagatt
    16741 tttgccatct gcattagaga aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg
    16801 aacaagacga ctggaagact ttcctttgtt gctcgttcga ataatctggt gatcttttct
    16861 ccgacaatcg ttcccatact tcccatgata aaattaggat ccatcactcc aataatgaag
    16921 ggaatcccct gaatggttgc tttaccagtg aggactgctt catgaagtcc tgttttatct
    16981 tgcatttttt taattttttc cgtatattcg ggaaatgcta gcggatcttt ggttacaaga
    17041 tccgtatccc attcttcaaa acttttttca tcaataatca aagctagacg ctgccatgca
    17101 ccgattcgaa aagcgtatcc gcaatgaggg catacttttt cttcaccgat gtctttcgta
    17161 taaaggatat gtttgcaatt tggacacttt gcccacatgt tatcaggaac agatggcgag
    17221 ttagcaggtt tgctctctct attaggattg atccgaatat atttcttttt cttaaacaga
    17281 gccatctatg cgcctcctat ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt
    17341 aaaaatgctg tgctgtaatc acctgctatg actgcaggat gactaatcaa atccatctga
    17401 aattctgcgt tcgtgatgat tccttctgtg ataaattcac ttaaggctcg ctgcatcttc
    17461 attaatgctt caaaacgcgt atttccatgg acgattattt ttgcaatcat tgaatcataa
    17521 tatggcggga tcgtatatcc cgggtaaact gcactgtcta ctcgcagtcc catcccacct
    17581 gctggaagca gtaagttttg gatcttccct ggagaaggag caaaatggaa agcaggattt
    17641 tctgcattaa tccggcattc gattgcgtgt ccttggaata ctacttcttc ttgacacaca
    17701 ttcaaaggct caccggctgc tatctccacc tgttttttta ctaaatcgat tcctgtgacc
    17761 atttctgtta ctggatgttc cacttggatt cgagtattca tttccatgaa ataaaaatcg
    17821 ccttcttcat ccatcagaaa ctcgatcgtt ccggcatttt cataattgac tgctttagct
    17881 gctcgaacag cagcgtctcc tagtgcttct ctttttgttt gtgagatcac tacagaagga
    17941 gattcttcca gtactttttg attgttgcgt tgtaaagaac aatctcgctc gcctaaatga
    18001 atgacatgac caaatcgatc acctaagatc tgaacttcta tatggcgagc aggataaatg
    18061 attttttcca aatacatatc atcatttcca aatgccgctg ctgcttcttg ctgagctgaa
    18121 gaaaaatgct gtgggagctc ttcttttgat tgaactttcc gaatgccttt cccgccacca
    18181 cctgcagctg ctttcagcat cactggataa ccaattctgt ctgctatttc caatgcttct
    18241 tctactgtag ataatacacc agtacttcct ggaatgactg ggacatttgc ttcttgcatc
    18301 aattgacgcg cattgatctt atttcccatt gcatcgatcg ttgcactttt aggaccaata
    18361 aatgtgatat tgcactcttc acacatggag gcaaatcggc tattttcaga taaaaaacca
    18421 aatcctggat gaattgcttc ggcttttgtc acgattgcag cgctcagtac ttcctgaaca
    18481 ttcagatatg aatcaacggc ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt
    18541 gtatgaagtg cttcggcatc tgcttctgaa tatatcgcta cggtttggat ccctagttcg
    18601 cggcatgcac gaatgatacg tacagcgatt tcaccgcggt tagctatcaa tactttagaa
    18661 aacatatttc acctatccaa tcataaaggt taattccgct tcggctactt ttttaccgtc
    18721 tactttagcg attcctttgc ctattccggc tgaagctttc actttcaata tctcaacttc
    18781 aaggtataag gtatctcccg gtgtaacttt cttacggaat tttgctttgt ccaatccgcc
    18841 gaaataagcc gtctttccct tgaattgttc aagagaaagt aatgcaactg ctcctgcttg
    18901 agccattgct tcaacaatca atacccctgg cataacaggt tcttctggaa aatgtccttg
    18961 aaaaaagggc tcattgatcg ttacattttt cttagcaacg atccgttctc cttcaaccat
    19021 ttcttccact cgatcaatca aaagcattgg ataacgatgt ggaatgattt cttttatttc
    19081 ttgtatgttc attgttttcc ctctttcctg ctttgcttat ttttgctgaa tcctaaagag
    19141 tggctgtcca tattcgacaa ctgactcgtt ttctacaagg atttctgtga tcactccgtc
    19201 ggcctcacta gttatctcat tcatcagttt catcgcttca ataatgcaga taacttctcc
    19261 tttggcaact tgatcaccta cttttttgaa agctggtttg tcgggcgctg gttgcagata
    19321 aacgattccc acgattggcg aaacaacctc gattccatca aatactgccg attctttttc
    19381 tttcattatt tctggagcag ttgttgcttg ttcaggtatg acattgactg gctccgaaac
    19441 tggcaacgtt tcgtttgttt gaaccatatt catgcctcga cttgttttat ttttattcat
    19501 atacaattca aattgacctt cacgcaaatc gaattccgtt agcgaggatt gatcaaattg
    19561 actgactaat tctctgattt cattgatatc caaaattatt cctcccagcg tttcatgcac
    19621 agaaccgcat tatgtccgcc aaaccctaat gaattactca ttgcataagt aaaacttgca
    19681 ggtttgcttt cgtttttgac aactgtcaag tcaatctcag gatcttgttt ttctacattg
    19741 atcgtaggtg ggataaattg atgttctaat gccagcaaag ttgccaaggc ttcgatcccg
    19801 ccggctgctc ccaataaatg tccagtcatc gatttcgtgc tgctgacgta agtatctctg
    19861 aaattttcac ctaatgcgat ttggattgct tttgcttctg cctggtcatt ggctggtgta
    19921 cttgttccgt gagcattgat atatccgact tgttccggct gtacaccagc ttcttccatc
    19981 gcaagcttca ttgctttaga tgcaccagaa ccatctggag taggagctgt catatggtaa
    20041 gcgtcacagt ttgcaccgta tccaacgatt tctccataga ttttggcacc gcgagcttgt
    20101 gcatgttcta aagattcaag aactagaacg cctgctcctt cacccatcac aaaaccattc
    20161 cgattttcgt caaacggaat cgaaccacga ttaggatcag ttgattctga gagtgctgtt
    20221 aaagctgcaa atccagcaat ccccatctca caaatcgttg cttctgttcc tccagcaaga
    20281 atgacatcag aatagccatg tttgatgtta cggaatgctt ccccaattga attcgttccg
    20341 ctagcacaag ctgttacgat agctgtacaa ataccttttg cacctacacg caaagcaata
    20401 tttccagctg ccatgttggc aatcgccatt ggtacgaaca ttggagaaac tcgttttgca
    20461 cctttttcat gtagacgaat aacttggttt tcgattgttg gaagaccacc gatcccagat
    20521 ccgatgatga cgccaaaccg gtcttgattc attttttctt tatctaaacc gctcatctcc
    20581 attgcttcta aagcagcgta gattccatag atcgaaaaca tatccattcg tttgctatct
    20641 tttttaataa aatatttatc gtaagggaaa tctttgactt cacccgctaa ggtaatccct
    20701 gtttctgaag catcaaattt agtgatcggt gcgatgccat tcgtcccctc ttttaagcta
    20761 ttcaaaaagc tgtctgcgtc atttccaata ggtgaagtga ctccatagcc agtaattacc
    20821 actcgattca tttgtttgct ctccttttct tatccgtgca tgaccaatcc gccatcgaca
    20881 ttgatgactt gtccggtgat atatggattt tgtgctaaga aaactgctgt ttgtgcaata
    20941 tcttctactt gtccgaaacg ctgcatcgga atttgctttt ccgcttgttc cttgactttg
    21001 tctgctaaaa cttccgtcat atcagttgtg atgaatcctg gcgcaatggc attacatgta
    21061 atgcctctag ttgcagcttc tcgtgccaca gattttgtta atcctactac gccagcttta
    21121 ctagctgcat aatttgcttg accaatattt ccaatcaacc cagatacact agagagatta
    21181 atgatggccc cttctcgctg cttcatcatt tttttcaata cgtgctgcgt catattgaag
    21241 gttccaatca aattgatatc cagacatttt ttgaaatctt ctgcatccat tcgcatcaca
    21301 agtttgtcat ttgtgattcc ggcattgttt actaacacat ggatcgatcc aagcttttct
    21361 tctgcttctt tgatcatttg tcccgctttc tcataatcag agatgtcacc ggaaactcct
    21421 acgcatttta caccgaatgc ttcgatttct tctatctttt cttttggaat ctctccgcga
    21481 ccattcaaaa tgatatttgc tccagctttt gcaaaagcta aggccatcgc tttgccgatt
    21541 cctcttgtac tacctgtgat aaatacattt tttcctttta catccataac gttcctcctt
    21601 atagtattgc tattgtttca tcaaatgtct gcttattctc aacacgcaga actggtattg
    21661 ttttatcgat ttttttcata aatccgctta atacttttcc ggggccgact tcgatgactt
    21721 gttcgatgcc gattgttttt aacttatgga tactctcata gaaacgaaca ggttgcatga
    21781 cttgctgttc aagtagtccc gcgattgttt ctttcttcat tatttccgtt gttgtgttgc
    21841 ttatgatagg aaaagaaggt tcagagaatt gaatctgact taggtccttc gccagttttt
    21901 ttgctgccgg ttctaaaata gctgtatgaa aaggcccgct gacatttaga gggatcattc
    21961 ttttgaaccc tttttctttt aatagtaaaa cagcttcgtc tactgctttt tcttcgccac
    22021 caatcacgat ctgctgaggt gtattataat ttgcaggaga tacgatgccg tacttgctag
    22081 cttcatgaca gctttcctcg atcgtttcaa tcggcgcatt catcactgca accattttcc
    22141 cgcttccagc tggggcagct tctgtcatat aagcgcctct ttttgctact aacgcaactg
    22201 cttcagtgaa ggatagcgca ttactagcta ctaatgccga atattctcct aaactcagtc
    22261 ctaaagctgc atctgggatg attccatgct ccttcaataa gcgataatac gcgatgctga
    22321 ctgttagaat agctggctgt gtatattgcg tctgatccaa acgttcattt tctgtaaagc
    22381 aaagttcagc catatcgtag ccgagaatct cacttgcttc atcaaatgtc tgctttacaa
    22441 cggcttcttc atataaatct tttcccatac cttgatattg tgcaccttgg ccactaaata
    22501 agaatgctgt tttcatgttt ttctccaata tatccagaat atatgtttga ttcttcctat
    22561 tcaaaaaggc cgagacattc ctatttcagg aaatgtccca gcccccaacc taattttaat
    22621 gattatgcag tttgtttttc tacgtaagtc actaaatctt gaaccgtttt gattccgtct
    22681 tctgtttcga ttttcacgtc aaattcatct tcaatttcat tgatgatttg gaataaatcc
    22741 aagctgtcag cttctaagtc ttcttggatg tcagtagtca atttgatttc ttcagtttct
    22801 tttcctaatt cttctgcgat gatttcttga attttttcga ataccataat aatatattcc
    22861 tccgatattg tttgcttttt atttttataa tttaacagtc tacaactcca tgagaatact
    22921 tccccatgtt aaacctccgc catagcctgt aaatattact ttctgctgtt ttcctaaggt
    22981 gatctttcca ctagaaactg cttcatccaa taagataggg atgcttgcag cagatgtatt
    23041 cccatattta tccatattgg tcaggaactt ttcttgcggc atctttactt ttcgggcaat
    23101 tttctcaata attctgaggt ttgcctgatg caaaagcaga taatcaactg gtttgtcttt
    23161 catgacttct tggatattgt ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc
    23221 agtcatttgc aaataatacg aaccttctga atcttctgaa tagaacggat ttttattttg
    23281 ctggtagcca gcggtcaatg agctacttct ggtaccgtca gctgctaatt tttctttcag
    23341 caattttttc tgtggattcg cttctaagag aatcccacct gctccatcgc caaacaacac
    23401 agcagtcgta cgatcagacc aatcgattag cttagatagt gtttcaccac caatcaccag
    23461 tcccttagtt gagccgcagc ggatcatttt ttcagccatg cttaacgcat aaacaaatcc
    23521 tgagcacgca gcactgatat caaaagcgat agcatgagtt gctcccaatt tcccttgtac
    23581 ttgtgctgct acagatggtg tcgtgtaatc gggcgacatt gttgctacta aaataaaatc
    23641 caaatcttta gggtttagat ttttctgttt tatcaatttt tccgccacta aaatgcataa
    23701 atcagaagtc tcctgatttg ttacacatct tcgttcacga atacccgtcc ttgacgaaat
    23761 ccattcatca gaggtatcca tcatcttgga aagatcatta ttagtaataa tcttttcagg
    23821 aaggacgctg gctgttgcag taatcattcc aaattgttcc attaaatatc ctcacttata
    23881 gtcttgcaag aagtcgtgca agtttttcag tgcatccagc aaggcatgct gttcctccgt
    23941 agccattccg tctaagatgt tcttgaccat ttcacgatga aaatgctgat ggacccgaaa
    24001 tagtaacttt ccttttttcg ttagtcccaa ctttacaacg cggcggtcat cttcactgcg
    24061 gatacgttcg acatagcctt tttttactaa cttgttaata gcagtggtca aggtcccaac
    24121 agtaattgat agttctttgg ctacttctga agttgttttt ttcttgtaca tcccgattgc
    24181 ctcgatcgtg tgcatctctg tgattgacag atctttgaat tgagattttt tcagctcaga
    24241 ctcttcgatt gtcaaaatat cattgaagac actaacaaga taatcattta ctgtttttaa
    24301 atctggttcc atgttccacc gtccactaat tagtttgatt atcaaatctt ttgatgttca
    24361 aactatatct taactttttt caaaatgcaa ccttaaaaga tcattttttt actatatgag
    24421 ctttttttaa gattttactc atttaaaaca actgataaat cagcatttat aatt
//
LOCUS       DAPCMU010000027        23913 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPCMU010000027 DAPCMU010000000
VERSION     DAPCMU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.027"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            69..821
                     /locus_tag="RD744_000914"
     CDS             69..821
                     /locus_tag="RD744_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0556934.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            818..1732
                     /gene="pfkB"
                     /locus_tag="RD744_000915"
     CDS             818..1732
                     /gene="pfkB"
                     /locus_tag="RD744_000915"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDU0556935.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1750..3681
                     /locus_tag="RD744_000916"
     CDS             1750..3681
                     /locus_tag="RD744_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0556936.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3792..5351)
                     /locus_tag="RD744_000917"
     CDS             complement(3792..5351)
                     /locus_tag="RD744_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDU0556937.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5506..7740
                     /gene="pcrA"
                     /locus_tag="RD744_000918"
     CDS             5506..7740
                     /gene="pcrA"
                     /locus_tag="RD744_000918"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDU0556938.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7757..9793
                     /gene="ligA"
                     /locus_tag="RD744_000919"
     CDS             7757..9793
                     /gene="ligA"
                     /locus_tag="RD744_000919"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDU0556939.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9899..10204
                     /gene="gatC"
                     /locus_tag="RD744_000920"
     CDS             9899..10204
                     /gene="gatC"
                     /locus_tag="RD744_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDU0556940.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10204..11676
                     /gene="gatA"
                     /locus_tag="RD744_000921"
     CDS             10204..11676
                     /gene="gatA"
                     /locus_tag="RD744_000921"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDU0556941.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11673..13103
                     /gene="gatB"
                     /locus_tag="RD744_000922"
     CDS             11673..13103
                     /gene="gatB"
                     /locus_tag="RD744_000922"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDU0556942.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13113..14168
                     /locus_tag="RD744_000923"
     CDS             13113..14168
                     /locus_tag="RD744_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDU0556943.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14279..15652
                     /gene="rlmD"
                     /locus_tag="RD744_000924"
     CDS             14279..15652
                     /gene="rlmD"
                     /locus_tag="RD744_000924"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDU0556944.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15655..15783
                     /locus_tag="RD744_000925"
     CDS             15655..15783
                     /locus_tag="RD744_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDU0556945.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16223..16411
                     /locus_tag="RD744_000926"
     CDS             16223..16411
                     /locus_tag="RD744_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDU0556946.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16489..17502
                     /locus_tag="RD744_000927"
     CDS             16489..17502
                     /locus_tag="RD744_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0556947.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17569..18603)
                     /locus_tag="RD744_000928"
     CDS             complement(17569..18603)
                     /locus_tag="RD744_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0556948.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18718..20943
                     /locus_tag="RD744_000929"
     CDS             18718..20943
                     /locus_tag="RD744_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDU0556949.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20961..22844
                     /locus_tag="RD744_000930"
     CDS             20961..22844
                     /locus_tag="RD744_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDU0556950.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKRSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22825..23799
                     /locus_tag="RD744_000931"
     CDS             22825..23799
                     /locus_tag="RD744_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDU0556951.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         7991 a         4373 c         5187 g         6362 t
ORIGIN      
        1 tttttgactg atttatattg ctagtagaaa aaaaatatgc tattatttgt attgaggtga
       61 tcgtgaaaat gcttacagag gaacgcagac aaaaaatcct agacttgctt gaacagcaga
      121 aaatcgtgaa atcacaagat cttgtaaacc ttttgaatgc ttctgaatca acgataagac
      181 gtgatttaca agagctggag aatagtgggt ttctagaaag agttcatggc ggagctaaga
      241 aagagcaatt attgaatttt gagcagaata tgagtgaaaa atcactcaaa aacgttcacg
      301 aaaaacagca aatcgccaaa tcggcagctg aaaccattaa tgacgaagat gtcatttatt
      361 tagacgctgg ctctactacg ttagaaatga ttccttttct gcaaggaaaa cagatcacag
      421 tagtgacaaa ttccgttcaa catgcggcaa atttagttga tttggagctt gctaccatta
      481 ttttaggtgg aacgataaag ctggcaacta atgctgtttt aggatctaat gcgatagaac
      541 aactaaaaaa ttttcatttt aataaagcat ttatgggaat gaacggtgcc catcttgaac
      601 aaggattcac gactccggat ccagaagagg cagctgttaa acgtttagcc atgaaaaatt
      661 ccgaagagac atttgtattg attgatcata ccaaattcaa ccagttgtcc ttcacctctg
      721 ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg tccattagaa ttcgttaatg
      781 aattccgtga aaagacaaca attaaggagg caatacaatg atttacacag taacacttaa
      841 tccatcaatt gattatatcg tacgcgtaga tgggttaaaa ctaggcgatt tgaaccgaat
      901 gaaggaagac ttcaagttac ctggaggaaa agggatcaat gtttcccgta ttttgaaacg
      961 gatcgattct caatctacag cactagggtt tttaggaggg ttcactggcg aatttatttc
     1021 gaagtggctt gaaaaagaac aaatccagtc tttgttcaca accgtaaaag aagatacaag
     1081 aatcaatatc aaactaaaat cagacactga aactgagatc aatggcttag gcccatctat
     1141 ttcagatgaa gaaatcgatg agttgaagca agcattagga acagtaggac aaggagatat
     1201 cgtcgtattg tcaggaagcg cgccagctag cctgcgaaaa ggattttatc aagaactgat
     1261 tgatatcatt cgagaaaaag gtgcagattt cgtgattgat acaacaggtg atgacctaaa
     1321 aaatgcactt gttaagaaac cgttgttgat caaaccaaac aatcacgaat tagctgagct
     1381 ttacaacaca accttccaat ctgtagaaga aatcattccg tatgggaaaa aattactagc
     1441 tgaaggggca cagaatgtgc tgatctcaat ggctggagac ggtgctttgc tcttcacgga
     1501 acaaggaact tataaaacga atgtattagt tcgcccattg aaaaattcag taggtgcagg
     1561 ggattcaatg attgctggct ttattggggc ttacagccgt acgaaagatc caatcgaagc
     1621 ctttaaatgg ggagttgctt gcggaagcgc aacagctttt tctgatgact tggcagctcg
     1681 cgaattgatt gatgagttgc tgccagaagt tgaaatcaca aaaattgatt agtttcaagg
     1741 aggataatca tgaatatcaa ggatttaatg gtcaaagaag cgatgatcat ggatttgcaa
     1801 gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa aaatgtttga tgccggccgg
     1861 atttccgata tcgaaacata taaagaagga attctagccc gcgaagcaca aacttctaca
     1921 ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca gtgcagttaa agaagcaaca
     1981 gttttattcg caaaaagcca aaaaggagta gattatgaag ccttagacgg acagccaact
     2041 tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg acacacattt gcaagcatta
     2101 gcagcactat ctcgattgct gattgatgca gatttcgtag gaaaattaaa agaagcaaat
     2161 acaccagaag aagtccaaga cttgttccaa acagctgaag agcaaaaaga agcagaagaa
     2221 aaagcagaac aagaaacaca agctgcagaa gcttctgctc atccagaaag aaaattcgtt
     2281 gttgccgtta cagcttgtcc aacagggatc gctcacactt acatggcaga agatgctttg
     2341 aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg aaacgaacgg ttcagaagga
     2401 atcaaaaacc gattgactgc tgaagacatc gcccgtgcag aaggtgtaat cgttgcagca
     2461 gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac tgatcaatcg tcctgtcagt
     2521 gatggtatcc gtaaaccaga agagttgatc aatctagccc ttagtggtac tgcaccagta
     2581 ttccatggtg atggaaaaga aagcagttca gaagaaggaa atgcggatgg tactatcgga
     2641 caaagaattt acaaagattt gatgaacggc gtatcacaca tgttaccttt cgttattggt
     2701 ggaggaatcg ctattgcgct ttctttcatg atcgaccaat ttattggcgt gccacatgat
     2761 caattatcaa atctagggaa ttacaatcaa gctgctagct ggttcaacca aatcggacaa
     2821 gctgcatttg gtttcatgct gccggtatta gcaggattta ttgcttcaag tatcggtgac
     2881 cgccctggtt tgatcgttgg atttgctgct ggtgcattag ctaatacagg cggtgcagga
     2941 ttcttaggtg cactgattgg tggattctta gctggttatg tcatcgtatt tttacgtaaa
     3001 ttattcaaga acttaccaaa atcattggat ggaattaaaa cgatcctctt ttatccagtg
     3061 tttggcttac tgattactgg tttcttgatg ttgctggtca atgtccctat gaaagcaatc
     3121 aatgatgcat tgaacggttt cttgacaagt atgagtggtt cgaatgctgc tttacttggt
     3181 gcattattag ctggaatgat ggcggcagat ttaggcggtc caatcaacaa agccgcttat
     3241 gtgtttggta cagcaacttt agctacgact gttgcaactg gcggaagtgt cgtgatggct
     3301 tctgttatgg ctggaggaat ggtacctcca ttggcaattt tcgttgctac acgtttgttt
     3361 aagaataaat tcagcaaaac agatcaagat gctggtttga cgaacatcgt gatgggacta
     3421 tcgtttgtca cggaaggatc gattccattt gcagcagctg acccgattcg tgccattcca
     3481 agttttatta ttggttctgc tttaactggc ggattagtcg gagctttcgg aatcaaatta
     3541 ctggctcctc acggcgggat tttcgttgta ttcttactga gtcacccggt catgtatttg
     3601 gtgtttattg ctatcggagc agtcgtatct ggtgtgattt atggtttatt gagaaaagct
     3661 cctgcagtag cagaagcata gaaataaaaa agaagaggct gggttttccc agcctcttct
     3721 ttttttatag aatcatatag aatcaagtag tgtagatgaa tagttgttgt aaaatagttt
     3781 ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag aagatttagc ctcgatttcc
     3841 tgcttgattc ttggcaagtt tgcttcatca gtcaagataa ccaaaatatc tcctacattc
     3901 atgatcgtat caccgtgagt caagatttcc tgatttcctc tgcgtataga gattagcagc
     3961 atatttttag gccaattgaa gtctcgtacc attaatccgt ctaaagaact ctctgccgtt
     4021 actggaaaat caaaagttgt ccttgcacct tttagtgagg cggtctgagt atttccaagt
     4081 agacgttcca ataatgattc gtaaattgga ttaccgccga gaaggtcatt gacgatataa
     4141 gcaacaagag cagccaatcc taaaggcatc agatgattaa gagagccaac catttctgtc
     4201 actaaaataa ttgctgtcag cggtgctttt ccaatcgctg tgaaataacc agccatggca
     4261 ataacgacaa aatccttgat gtagattgga tccatcccga aatcaaccat aatgtttcca
     4321 taaaagctac cgattaaagc ccccaatgtc aatatcggaa ggaaaatccc ccctggaaga
     4381 cccccaccgt aagaaatcat ggaaaaaacg aatcgaagaa caaacaaacc gataatcata
     4441 atgattggca aggaagcatg tccaaaagct aaaacgattt gattgcctcc gcctagagta
     4501 ctaggccata aataaccaat tggcaaaatc agcaagaaag gaacgaaccc gtgaaaataa
     4561 gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca gcaagacatt ctgataaata
     4621 aaacctaaga tgcctagaag tactcctaag atcactaagg aaccataata tttcaacggc
     4681 aaagaaggaa ggtcacctag atataaaaca ggtctcaagc cgaaaaagta aagagagacg
     4741 aagttagcag tgattgctga agcaaatgat gtcagccaaa caagtggaga aaaggagtga
     4801 tggatttctt ctaaaacaaa gagtaatcct gcgataggag cattgaatgc tgctgcaagt
     4861 ccgctacttg caccgctaga aattaaaatc ttttcttctg aagatggact ccgaaacaat
     4921 tgtccagctc cttgtccaat ggatgcgcca agctgtatag aaggtccttc tctacctaga
     4981 aaaaggcccg caccaactga aagtacaccg ccaatgaact ttttccataa aacagagaac
     5041 cagttcattg ataattcccc gcgaagctgt ccctctactt gtggaatccc gcttcccttg
     5101 atatccggtt catttttgac cagataaccc acgaaaaatg ctgcaaggat gcagcaaata
     5161 gctaggccaa ttaagtaaat cggctgttcc cgactcattt catagatact tcgaactaaa
     5221 tcgcttagct tttcgataga taatcgaaac aggctaacga cgattcctgt cagcagtcca
     5281 acaaatattc cttttagaat aaaatatatc tgagtcttat ctaatttctt gatttttcct
     5341 tttttttcca ttcaaacgcc tcgctttctt ttaatttagc gcgaaatcaa cgaattgaca
     5401 aggaaaaatc cataaaaaga aacaaatgat tttgaactag ctagtttaat gaaagccaag
     5461 aaaatgctat aattaacaag attgataaaa gaaaggatgt tgttaatgtc atccaaagct
     5521 gaattattaa acgggatgaa cccccgtcag aaagaagctg ttcttcacac tgacggacca
     5581 ttactgctaa tggcaggtgc aggaagtgga aagacgagag tgctgacaca ccgcattgcc
     5641 tatttgattg aggaaaaaga agtcaatcct tggaacatcc tagcgattac ttttaccaat
     5701 aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc tcgcatctgg aggcgaagat
     5761 gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt taagaagaga tgttgatttt
     5821 atcgggtata accggaactt tacaatcatc gattcttctg aacagctgac cttgatgaaa
     5881 cggatactaa aagaactgaa cattgatccg aaaaaatatg atcctcgaag tatattagga
     5941 acaatctctc aagcaaaaaa cagcctgcag acccctcagg attttgcaaa aatgcaaggc
     6001 agttattacg aagagattgc ggcaaaatgt tatgctgctt accaaaaaga gcttcaatat
     6061 aatcaatgta tggactttga cgacctgatc atgaatacga ttcgattatt tgaagaacat
     6121 cctgattcac tcacgtatta tcaaaacaaa ttccattata ttcatgtaga tgagtaccaa
     6181 gatacaaatc acgcacagta tacgctagtc aatttattgg caggtagatt taggaacctg
     6241 tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc gcggtgcaga tatgcagaac
     6301 atcttggatt ttgaaaaaga ttatccagat gctgcagtaa ttttgctaga acaaaactat
     6361 cgctccacga aaaacatcct tagcgcagca aaccaagtca ttgaaaacaa tagcaatcga
     6421 aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca agatcactta ctaccgtgca
     6481 gataatgaac gagacgaaac ccgttttatc gtagaccgga tgcaagaaga gattcgaagc
     6541 aatcatcgga attacggcga ctttgccatc ttgtaccgga cgaacgcaca atctcgtgta
     6601 atggaagaaa ctctgttgaa agcaaatatt ccttataaaa tggttggagg gcacaagttc
     6661 tacgatcgta aagaaatcaa agatatttta gcgtatttga atgtgctggt aaatcctcaa
     6721 gattcaatca gttttgagcg tatcgttaat tcgcccaaac gaggcatcgg accaggatct
     6781 atcgaaaaac tacgcagttt tgcttctctt catgaatggc cactgcttga agcagcgcaa
     6841 aatgtggatc tggcaaatat cggtggaaaa gcaggacagc aattaggtgc atttggagag
     6901 atgatccaag aagtcactca gatgatccca tacttgacag tgacagaatt gacgaaagag
     6961 gttttggatc gtagcggata tctagaagac ttaaaaatcc aaaatacatt agaagcacaa
     7021 gcacgcatcg aaaacttgga ggaattttta acggtcacac aagaattcga caaacaattc
     7081 gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa aactaacggt cttcttaaat
     7141 gatctggcac ttgtttccga cattgacaat ttagaagaag atgcttccca agtgacattg
     7201 atgactttgc atgcagccaa aggattagaa tttccagttg tattcctgat tggtctagaa
     7261 gagggggttt ttcctttatc tcgagcattg atggaagaaa gcgaactgga ggaagaacgc
     7321 cgtctcgcat atgtggggat cacgcgagca gaagaagcgt tgtatttaac aaatgctttc
     7381 tccagaacat tatatggacg gacgcaatat aaccgacctt ctcgttttgt agaagaaatc
     7441 gatcaggagt tactggaaat cgaaggcatg cgtcctacgc ctaaaaaaac gcctgtattc
     7501 gctaaaaaaa cagcatatag ctataaacag ccagagacag cggttgttcc ctctaaaagt
     7561 gctactggag gggaaaataa cagctggaaa cctggagata aagtcaaaca caaaaaatgg
     7621 ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg atctagaact agatgtggct
     7681 ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg cgccaattga aaaagcttag
     7741 tagtggaggg taaatgatgg aacatgaaat gacgattgac gaagctgcta aacgagcaga
     7801 agaacttcgt acaagactga accaatggtc tcgcgaatac tacgtagaag ataaaccaac
     7861 agtggaagac tatgtttatg ataaagaata cgcagaatta gtagcaatcg aggaacaata
     7921 tcctgatttg atcacgtctg attctcccac tcaacgagta ggcggaaaag tattagaagg
     7981 ttttgaaaaa gtcactcatg atattccgct ttacagtttg aacgacgttt tcagcaaaga
     8041 agagctgata gcttttgatc aacgtgtaca aaaagcagtg ggacgtgtag ttgactattg
     8101 ctgtgaacta aaaatagatg gactttccgt ttctttgcgt tatgaagatg gaaatttcgt
     8161 gagaggtgcg actcgcgggg atggaactgt aggagaaaac atcactgaaa acctaaaaac
     8221 tgtccggtcg gtcccaatca aattaaaaga accaatgaac atcgaagtaa gaggcgaatg
     8281 tttcatgcca aaacggtcat ttgtccaatt gaaccaagac agagaagcag agggaaaaga
     8341 tatctttgcg aatcctcgga acgccgcagc tggttctttg cgtcagttag attctaaaat
     8401 cacagcgaaa agaaatctgg acactttttt atacacagta gctgatttcg gtcctatgca
     8461 agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa attggattcc acacgaatcg
     8521 ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca tacattgaag aatatcatga
     8581 caaacgtgtg gatttgccct atgaaatcga tggaatcgtc atcaaagtga atgaattctc
     8641 gctacaagat caacttggtt ttactatcaa agcaccgaga tgggcagcag cttacaagtt
     8701 tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa tggacagtcg gtcgaacagg
     8761 ggtagtaacg ccaacagcta tcatgacacc agtccgagta gcaggaacga cagtaagccg
     8821 tgccagcctg cataacggtg attatattaa gttgaaggat atccgcttga aagatacagt
     8881 tctgatttat aaagcgggag acatcatccc agaagtttcg caagtcgtgt tagataaacg
     8941 acctaaagac agcgaagaat accaattgcc tacacattgt ccagtttgtg gaagtgaact
     9001 tgtccatttg gatgaagaag tagccttacg gtgcatcaat cctaaatgcc ctgctcaaat
     9061 gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg aatatcgatg ggttggggcc
     9121 gcgcgtacta gaacagatgt atgataaaaa actagtagca gatgttgctg atctttacaa
     9181 attgacagaa gaagagttat tgacgttgga caaaataaaa gaaaaatcag caaataatat
     9241 tttgacagca atcgacaaca gcaaagataa ttcggtcgag cggttgattt tcgggttagg
     9301 aatccgacat gtcggagcga aagcagcaaa aattttagca gaacatttcg gtgatctaga
     9361 aacattgagt aaaagtgact atgagtcgat tattgcgctt gatacgatcg gtgatatcat
     9421 tgccgacagt gtagtcactt atttcagtaa cgaagaagtc catgaattga tgaatgaatt
     9481 aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga aatgcccaat tgcaagaagt
     9541 cgagtctcct tttaaagaaa aaacagtagt ccttactggt aaactaacac gatttactcg
     9601 tgaagaagca aaagagacga tcgaaaatct tggcgggaaa gtgactggta gcgtatcgaa
     9661 aaaaacggat atcgtggtag caggagaaga tgctggcagc aagctgacca aagcacaaga
     9721 attaggaatc gaagtttgga cagaagatca aatggcagat gcattggcta aaagtcgctc
     9781 agtagaagaa tgaaaaatga gttttttcag acaaatttca ataaacaatt tcagacacgg
     9841 cactcttgtg ttaaaataga gagcaaagtt tttaactcgt ttagaaagaa ggggtagtat
     9901 ggcgattagt gaagaacaag tgaaacacgt tgccaaatta gcaaaacttg cgttcgcaga
     9961 tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc atcgatatgg ttgaaatgct
    10021 ggaagaagta gatacggaag gtgttccatt tacgtcgaat gtggttgaaa cagtaaacgt
    10081 gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat gaattattga aaaatgtgcc
    10141 ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc gataatgggg aggctggcgc
    10201 ataatgacag aattacacaa tcgttctcta gaagaactgc atcatatgct tgtatcgaaa
    10261 gaaatcactg tacaagattt gacaaaaaca acatttgaac gaatcaaaga aacagagcct
    10321 gaggtcgagg cattcattac attgaacgaa gaaaaagcat tggaacaggc aaaagcttta
    10381 gatgaaaaag ggattgatga aagcaacgtc cttgctggta ttccaattgg tatcaaggat
    10441 aatatcgtaa caaaagatat cttgacaacc gctgcatcta agatccttta caattttgaa
    10501 ccaatttatg atgcaacggt catggataaa atctatcaag cagatatgat ccctgttggt
    10561 aagctaaaca tggatgaatt tgctatgggt ggaagtacag aaacttctta tttcaagaaa
    10621 acaaaaaatg cttgggaccc aacaaaagta cctggtggct cttctggtgg ttctgcagca
    10681 gcagttgcag ctggacaaat tccagtttct ttaggtagtg atactggggg aagtatccgc
    10741 caaccagcag cttttaacgg catcgtaggg atgaaaccta cttatggccg tgtctcacgt
    10801 tttggtttga ttgcctttgc ttcatctctt gaccaaatcg gaccaatgac ccgtacggtg
    10861 aaagataatg cattagcatt gacagctatc agcggttatg acgaaaaaga cggtacttct
    10921 tcaggcgttt cagttcctga ttttgcagag ggactgacag gcgacatcaa aggaatgaag
    10981 attgctttac ctaaagaata cttaggcgaa ggtgtggccc ctggcgtaaa agaagctgta
    11041 ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg tggaagaagt cagcttgcct
    11101 cattcaaaat acggtgtggc tgtttattat atcattgcct catcagaagc aagttccaat
    11161 ttgcaacgat tcgatggtat ccgctacggt tatcgctctg aaaatgtgaa atcattagat
    11221 gatgtatatg tcaactcacg ttcagaagga ttcggtgaag aagtaaaacg ccgtatcatg
    11281 ttaggtacat tctccttaag cgcgggttat tatgacgctt acttcaaaaa agcaggacaa
    11341 gtccgtacat tgatcaaacg agattttgaa aaagtctttg cagattatga cttgatcatc
    11401 gggccatctt caccaacagt tgcattcgga atcggtgaga acattaacga tccaatcaca
    11461 atgtacatga gtgacatctt aacgatccct gtcaacttgg ctggacttcc aggtatgtct
    11521 gtaccggcag gtttatcaga aggattacca gttggattgc agatcatcgg taattatttt
    11581 gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac aagcaacaga attccataaa
    11641 caacaaccag ctattttagg agggaaagac gaatgaattt tgaaacagtc attggactag
    11701 aagtccacgt cgagttaaaa accaactcaa aaattttctc tccagcgcca gctcattttg
    11761 gtgcagaacc aaacagcaat acaaacgtga tcgactgggg ttatccaggc gtgctgccag
    11821 taatgaataa agctgcgctg gaattcggga tgaaagcagc tttggcatta aactgtgaaa
    11881 tctctaaaga tactcatttt gaccggaaaa attactttta cccagataat ccaaaagcat
    11941 atcaaatttc ccaatttgat caaccaatcg gtcatgacgg atggatcgaa atcgaagtag
    12001 aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt agaagaagat gctggaaaaa
    12061 acatgcatgg tattggcggt tattcttacg ttgatttgaa ccgacaaggc acgcctttga
    12121 tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga agcatacgct tatctagaag
    12181 cattgcgatc aattatccaa tttacagaag tcagcgatgt gaaaatggaa gaaggctcta
    12241 tgcgctgtga tgccaatatt tctcttcgcc cttatggaca agaagagttc ggtacgaaag
    12301 cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa aggtttagct tttgaagaaa
    12361 aacgtcaagc aaaagttctt ctttcaggag gagaaatcca acaagaaaca cgccgttttg
    12421 acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga agggtctagt gattatcgtt
    12481 acttcccaga gcctgatatc ccgaaattcg tgatcgatga cgaatggatt caaaaagtaa
    12541 aagctagttt gcctgagatg ccagcttctc gtcgcgaacg ctatatccgt gagtttggac
    12601 ttccagagta tgacgcaatg gtattgacac ttacaaaaga aatgtctgat ttttttgaag
    12661 ctgttttgga aaatggtgca gatgccaagc aagcatctaa ctggttgatg ggtgaagttt
    12721 ctgcttactt gaacagtgag aaactggaat tagctgagac aaaattgaca cctgagaact
    12781 tagcaggaat gattcgtttg atcgccgatg gaacgatcag ctcaaaaatc gccaaaaaag
    12841 tcttccgtga attgatcaca aatggcggag aagcccaaga agtagttgaa aaaaatggtt
    12901 gggtgcaatt atctgatccg aacaaactac tgccaatcat taatgaaatt ttggataaca
    12961 accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg tgcggttggc ttcctagtcg
    13021 gtcaaatcat gaaagcaact aaaggacaag caaatcctgg tgtcgtgaat aagctactta
    13081 aagatgaatt agcgaaacga tagaggctga aaatgaaaaa agcacgcgta atttataacc
    13141 ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga tatcctacag gcattagaag
    13201 aagcaggatt tgaagccagc gcctatgcaa ctacaccaga accagattca gccaagaatg
    13261 aagcatgtag agtcgcagaa ctaggttttg atctagtagt ggcagctggt ggcgatggca
    13321 caatcaacga agtggttaat ggaatcgctc cgcttgaaca ccgtcctaaa atggccatca
    13381 tcccagctgg tacaacgaac gactatgctc gagcattaaa aattcctaga gataatatta
    13441 aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa aatggacatc ggcctttctg
    13501 ataacagtta cttcatcaat atcgctgcag gtggctattt gacagaattg acttatgaag
    13561 ttccatcaga actaaagagt atttttggtt atcttgctta tcttgcgaaa ggagcagaga
    13621 tgctccctcg agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg gaatatatcg
    13681 gaaatgcttc gatgtttttc ctagggctga cgaactctgt tggaggattc gaacagatcg
    13741 cgccagatgc caaattggat gatgggaagt tttctttaat cattgtgaag acagcaaata
    13801 tctttgagat cttgcatatt gcagcactca tgctgaatgg cggtcgacat gtcgatgatc
    13861 caagaataat ctataccaaa acaagcagtt tgttcgttga aacagaagat gatatcaatc
    13921 gaccagtaat gatcaactta gatggtgaat acggcgggga agcaccgatg catttccaaa
    13981 atcttcatca acacattgag ttctttgcca ataccgatca gattcctgat gaagcaatca
    14041 caggtacaga tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa gaagtcgaac
    14101 gtttaacgga tgaagatatc gacggtgacg gaaaaatagc agacgaaaca cctaatcaaa
    14161 agaattaatc aaaagccggt gctgaacgta gtaaggtcag tgccggtttt agttgtgtaa
    14221 ttagctgatt tatagtagag ttcagtagga ttgaaaaata ggcttaaagg agcaaaatat
    14281 gacagaacag caaatagtaa agaaaaacga acgatataca gtcgaaatcg tcgacttgag
    14341 ttatgaagga atgggggtag caaaaatcga tggttatcca ttatttatcg aaaatgcact
    14401 ccctggagaa gaagtagaaa tactagtagt aaaagcaggc aataagtttg gatatggaaa
    14461 agtcgaaaca tttattacac aatcacctga tcgtcagcct gtagaaaaca atgcattact
    14521 tcgcataggt atcgcacctt tagcccattt gaagtatgaa caacagcttg tcttcaaaca
    14581 aaaacaagta gagcatgtaa tggcgaagat tgctaagatg ccagaagttc ctgtacttcc
    14641 aacggtcgga atggcagaac cattcggcta ccgtaataaa gcacagatcc ctgttcgccg
    14701 tatcgatgga cagttagcca ccggttttta caaaaagaat agccatgatc tagtccctat
    14761 cgaagatttt tatatccaag atcctgtcat cgatcaagca atcaaagtag tcagagatat
    14821 cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa aatgaaggac agcttcgtca
    14881 catcattatc cgtcgtggtc attattccca tgaaatgatg gtcgttttag ttactcgtaa
    14941 agaaaaattc tttaaatcca aggaaattgc tgaagctatt catgaagaat tgccagaagt
    15001 ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat gtgattttag gagacaaaga
    15061 aaaagtttta tatggcagag actatatcga agatcagcta ttaggaaaaa cctatcgaat
    15121 ctcagcaaag tcattttatc aagtaaatac tcaacaagca gagtatctgt accaaacagc
    15181 catcgagttt gccgagttag ataaaaaaga cattgtgatc gacgcttatt caggaatagg
    15241 tacgattggc ttgtctttag cagacagagt ggataaggtg tatgggatgg aagttattcc
    15301 agaagcaatt gaagatgcac agttcaatgc tttaaataat aaaatcgaaa atgttcatta
    15361 tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga tcaaaccatc
    15421 tgtgatcgta gttgaccctc cacgaaaagg attagatcat cattttattg aatcagcaat
    15481 cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt ttgcaagaga
    15541 tgctaagtta tttgcagaag caggttatgc cgtgaagaaa gttcagcctg tagatctttt
    15601 cccgcaaacc catcatatcg aattagtcgc ttcattcgtt aaaggagaat aaaaatggac
    15661 caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag gtggaggccg atccaaacaa
    15721 atggaaacac ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat ctttaataaa
    15781 tagacgtgct aaagaaagac taagaagtgt taacttttgt tattgtttct tagtcttttt
    15841 agttagtcta cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa
    15901 atctgggtca gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac
    15961 tgattgatca aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat
    16021 gtttgacgcg gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag
    16081 acccattatt ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt
    16141 gctgggagtt ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt
    16201 gactgtaacg aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga
    16261 agcgacacga tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc
    16321 aacagaagaa gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc
    16381 attgcagaag aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa
    16441 cactatggta taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa
    16501 attcttaaat ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt
    16561 cttgccttcc agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat
    16621 actgttcaaa tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa
    16681 acagatttga cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa
    16741 aacagcaagg ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga
    16801 gataaatcgg gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg
    16861 gttgcgatga attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg
    16921 aatgaactga tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa
    16981 caattatttt ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata
    17041 caaacgaatg atgcgtatgc taaacaaatg aaaatcaaac agctacttcc aaaagatagt
    17101 ctgaaaggaa ccatttctta ttgcggacct gttgatttga agctaacagc acagcagcat
    17161 tcggatgatc gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca
    17221 aaagattgga aaacgagtga gcaattgaaa gaagctagtg tggctgacca tgtttctaag
    17281 gaatttccgc caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa
    17341 gcattagaaa atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat
    17401 acaaaaaaag agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat
    17461 tgttaccagc agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg
    17521 tttcgttttg agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt
    17581 tgttcttggg ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg
    17641 ttcgtcccgt ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga
    17701 tttcgcattg atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt
    17761 atcaaagctt gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg
    17821 atagacacca attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc
    17881 attttctaat agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg
    17941 ataagacata tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct
    18001 cttttcattt atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc
    18061 gatataggct attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat
    18121 ccgttcaaga tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac
    18181 gactatttgc atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac
    18241 aacgatcacc gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt
    18301 atagattcga ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa
    18361 atgtttgtaa tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat
    18421 cgaaggcatt ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc
    18481 tgcctctact atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa
    18541 cacacgggaa actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac
    18601 catttttaca cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac
    18661 agtatacagg aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg
    18721 gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc
    18781 ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg
    18841 gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga
    18901 agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat
    18961 acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc
    19021 attcagcgca agaatggatc agacttactg gatctgagat atgacagcca ttatattaca
    19081 gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca
    19141 caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat
    19201 accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga
    19261 gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga
    19321 tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa
    19381 tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg
    19441 aataacttta ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga
    19501 cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa
    19561 ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag
    19621 tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt
    19681 cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa
    19741 cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc
    19801 aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc
    19861 tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc
    19921 aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt
    19981 ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct
    20041 gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg
    20101 gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat
    20161 acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg
    20221 attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat
    20281 gaactacttg aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt
    20341 ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat
    20401 aacacggatg cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt
    20461 tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg
    20521 ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca
    20581 aagctgtctc ctgaagaaaa aaagcaagta aaagaccaaa ttatatccta tcaaacgatt
    20641 cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt
    20701 gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata
    20761 ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca
    20821 ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat gacagttgga
    20881 ctttacttcc ccgattttca aggtgatttc caaacagaac tgcttcattt caaaaagtta
    20941 tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt
    21001 ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca agggaagaag
    21061 tagaaaccac atcctccctt aaacggtctt taaatggatc atggcgtttt tgttattcgc
    21121 caaatccttc ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc tgttcttttg
    21181 atgaaatcac tgttcctagt catattgaat tagcaaatta tgacaagatc cattacatca
    21241 atacgatgta tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg acaagccctg
    21301 agaaagaggc ggaagggatc ttttcagctg ctaaagacaa tccggttggt tcgtatttga
    21361 cgacttttcg attagaagaa ggtctaaagg gaaaacaaat ctcgattcat tttgctggtg
    21421 tagaacaagc aatgtttatt tggttgaatg gtcattttat cgggtatgcc gaagattcgt
    21481 tcactccgtc tgaatttgat ttaacacctt atttggaaga agaaaatatt ctagctgtgg
    21541 aggtttacaa aagaagtaca gctgcattct tggaagatca ggactttttc cgattttttg
    21601 ggatcttcag agatgtcttc ttagttgctc gaggacaaaa ccatttagaa gatctttgga
    21661 tcaaacctcg cttagatgac aaatgtcaaa atggcaaact tgaagtagat ttgtcttttg
    21721 agcaagtagc caaaaacagt cagctgaatg tagcgatact agatcctgat gggaaggaaa
    21781 tctatcaaca gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat ggatttgaaa
    21841 atgtggcact gtgggggcat tttcagccca atttatataa gcttgaattg gagacgatag
    21901 acgaagaaaa aagagtaacg gagtatattg actattcctt cggttttcgt aaaatcgaaa
    21961 tcaaagataa aatcatctac ttcaatggaa aacggatgat tatcaacggg gtaaatcgac
    22021 atgagtggca tccgaaaaaa ggacgcgcca tcactataga agatatggaa aaagatttgg
    22081 ctatcataaa aaatgcaaag atcaacggcg tacggacatc tcattacccc aaccagatca
    22141 cttggtatta tttatgcgac caagcaggaa tccatgtgat ggcggaaacg aacctcgaat
    22201 cccatggctc atggcaaaaa atgggcgcat ttgagccttc ttacaacgta ccaggtgcag
    22261 tacctcagtg gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa acattaaaaa
    22321 accatccaag tattcttttt tggtctcttg gaaatgaatc ttatgcggaa gaaaacattc
    22381 gacaaatgaa cgattacttc aaaaaagtag accccgatcg acttgtccat tatgaaggga
    22441 attttccaaa tccagcattc gaagattcga tttcagatgt aaaaagctgc atgtatgcgc
    22501 caccagaaga gatcattgac taccttgaaa ataaagcgga aaaaccgttc atcttatgtg
    22561 aatatatgca tgacatgggg aattcgttag gtggaatgga ttcttacatg acgttgcttg
    22621 atcgttatga aatgtaccaa ggaggctata tatgggattt taaagatcag gcgatccaag
    22681 tgactgatcc aatcacaggc agagaagtga tgcgctatgg cggtgacttt gacgataaac
    22741 caagtgacta tgaattctca gggaatggga tcgtttttgc agatggacaa gaaaaacccg
    22801 ccatgcagga ggtaagatat tattatgaaa aatacagtaa ataaaagtca tatggatacg
    22861 gaaaaagttg caatcgtctt cggcgactgt acattaggtg tcaaatcggg gaatacgcat
    22921 tatatttttt cttatacaag aggcggactg gaatcgctca ataaaaacgg aaaagaatgg
    22981 ctataccggg aaacacttcc agttttttgg cgagcaacga ccgataatga cagaggtagc
    23041 ggatttggat ttaaatccgg tatgtggtta ggagccggat tgtatccaaa agtcaaagaa
    23101 atagaagtac tggtggacaa taaaaagatc gatttgccga ttgctccagt aaataatcaa
    23161 tattcaagta atgaatacgc tgaaacggtt gaaatcattt ttacactgga aatcaacaca
    23221 gtaccaaaaa cccaagtgac aatttcctac caaatcgctg gagagggtaa aatgcttgtc
    23281 aatatgcatt attatggaca aaaaggatta ccggaattac ccgcattggg tttaaggttc
    23341 attatgccaa cgaaagcaaa attttttgag tatgaaggcc tatcagggga aacatatccg
    23401 gatcggatgg ctggtggtgt cgaaggtatt tataaagtgg caggactccc cgtttcccca
    23461 taccttgttc cccaagaatg cggcatgcat atggaaacga aatgggtaga actaacaaga
    23521 aatcaaacgt tggataatac ggataaagat caagaagatt tttcgttgcg atttgaacag
    23581 caggatacgc catttgcttt ttccgcttta ccattcaaag cagaagaact ggaacaggca
    23641 acacatatag aagaactacc gccagaaaga cgaacggttc ttacgatttt tgcaaaagta
    23701 agaggagtcg ggggtatcaa tagctgggga gcggatgttg agtcacctta ccgaatcagc
    23761 agcgaagagg accatgaaat cggctttatt attcagtagc aatctttaga ttattatttg
    23821 aaaaacggag tttacaagac aaagctgctt tattctatat gtcatgaaca agagagtaaa
    23881 aaagttctca gatacttttg ttcatgacta ttt
//
LOCUS       DAPCMU010000028        23648 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPCMU010000028 DAPCMU010000000
VERSION     DAPCMU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.028"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(55..789)
                     /locus_tag="RD744_000932"
     CDS             complement(55..789)
                     /locus_tag="RD744_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDU0556952.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="RD744_000933"
     CDS             1116..1526
                     /locus_tag="RD744_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDU0556953.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="RD744_000934"
     CDS             1519..2220
                     /locus_tag="RD744_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDU0556954.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="RD744_000935"
     CDS             2220..3806
                     /locus_tag="RD744_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0556955.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="RD744_000936"
     CDS             3825..4046
                     /locus_tag="RD744_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDU0556956.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="RD744_000937"
     CDS             4043..4804
                     /locus_tag="RD744_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDU0556957.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="RD744_000938"
     CDS             5184..5693
                     /locus_tag="RD744_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDU0556958.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="RD744_000939"
     CDS             complement(5768..6307)
                     /locus_tag="RD744_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDU0556959.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="RD744_000940"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="RD744_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDU0556960.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="RD744_000941"
     CDS             7320..8171
                     /locus_tag="RD744_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDU0556961.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="RD744_000942"
     CDS             8199..8834
                     /locus_tag="RD744_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDU0556962.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="RD744_000943"
     CDS             8854..9627
                     /locus_tag="RD744_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0556963.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="RD744_000944"
     CDS             10035..10832
                     /locus_tag="RD744_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDU0556964.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="RD744_000945"
     CDS             10810..11223
                     /locus_tag="RD744_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDU0556965.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="RD744_000946"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="RD744_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDU0556966.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="RD744_000947"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="RD744_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDU0556967.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="RD744_000948"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="RD744_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDU0556968.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="RD744_000949"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="RD744_000949"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDU0556969.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="RD744_000950"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="RD744_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDU0556970.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="RD744_000951"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="RD744_000951"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDU0556971.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="RD744_000952"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="RD744_000952"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDU0556972.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="RD744_000953"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="RD744_000953"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDU0556973.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="RD744_000954"
     CDS             21907..22485
                     /locus_tag="RD744_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0556974.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="RD744_000955"
     CDS             22537..23019
                     /locus_tag="RD744_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDU0556975.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="RD744_000956"
     CDS             23136..23510
                     /locus_tag="RD744_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556976.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7728 a         4272 c         5066 g         6582 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgat
//
LOCUS       DAPCMU010000029        23404 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPCMU010000029 DAPCMU010000000
VERSION     DAPCMU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.029"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            40..1701
                     /locus_tag="RD744_000957"
     CDS             40..1701
                     /locus_tag="RD744_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0556977.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1771..2118)
                     /locus_tag="RD744_000958"
     CDS             complement(1771..2118)
                     /locus_tag="RD744_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDU0556978.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2202..3143
                     /locus_tag="RD744_000959"
     CDS             2202..3143
                     /locus_tag="RD744_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556979.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3144..4187
                     /locus_tag="RD744_000960"
     CDS             3144..4187
                     /locus_tag="RD744_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0556980.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4193..5263
                     /locus_tag="RD744_000961"
     CDS             4193..5263
                     /locus_tag="RD744_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556981.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5253..6206
                     /locus_tag="RD744_000962"
     CDS             5253..6206
                     /locus_tag="RD744_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0556982.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6218..7228
                     /locus_tag="RD744_000963"
     CDS             6218..7228
                     /locus_tag="RD744_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0556983.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7513..8334)
                     /locus_tag="RD744_000964"
     CDS             complement(7513..8334)
                     /locus_tag="RD744_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDU0556984.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8431..8709)
                     /locus_tag="RD744_000965"
     CDS             complement(8431..8709)
                     /locus_tag="RD744_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556985.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8977..10986
                     /gene="metG"
                     /locus_tag="RD744_000966"
     CDS             8977..10986
                     /gene="metG"
                     /locus_tag="RD744_000966"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDU0556986.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11465..12052)
                     /locus_tag="RD744_000967"
     CDS             complement(11465..12052)
                     /locus_tag="RD744_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556987.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12227..13549
                     /locus_tag="RD744_000968"
     CDS             12227..13549
                     /locus_tag="RD744_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0556988.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13704..14480
                     /locus_tag="RD744_000969"
     CDS             13704..14480
                     /locus_tag="RD744_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDU0556989.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14473..15042
                     /gene="rnmV"
                     /locus_tag="RD744_000970"
     CDS             14473..15042
                     /gene="rnmV"
                     /locus_tag="RD744_000970"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDU0556990.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15035..15919
                     /gene="rsmA"
                     /locus_tag="RD744_000971"
     CDS             15035..15919
                     /gene="rsmA"
                     /locus_tag="RD744_000971"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDU0556991.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16370..16999
                     /locus_tag="RD744_000972"
     CDS             16370..16999
                     /locus_tag="RD744_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556992.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17123..18232)
                     /gene="ugpC"
                     /locus_tag="RD744_000973"
     CDS             complement(17123..18232)
                     /gene="ugpC"
                     /locus_tag="RD744_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDU0556993.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18441..18863
                     /gene="mgsA"
                     /locus_tag="RD744_000974"
     CDS             18441..18863
                     /gene="mgsA"
                     /locus_tag="RD744_000974"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDU0556994.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19186..19722
                     /locus_tag="RD744_000975"
     CDS             19186..19722
                     /locus_tag="RD744_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDU0556995.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19762..21525)
                     /locus_tag="RD744_000976"
     CDS             complement(19762..21525)
                     /locus_tag="RD744_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556996.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21528..23243)
                     /locus_tag="RD744_000977"
     CDS             complement(21528..23243)
                     /locus_tag="RD744_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0556997.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7533 a         4288 c         4768 g         6815 t
ORIGIN      
        1 attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt
       61 ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca
      121 agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg
      181 cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca
      241 ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca
      301 ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga
      361 gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg
      421 caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa
      481 aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt
      541 ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt
      601 gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat
      661 gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga
      721 ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga caaagataca
      781 gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta
      841 ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca
      901 aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa
      961 cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc
     1021 gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg
     1081 atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga
     1141 aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa
     1201 ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa
     1261 gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca
     1321 acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta
     1381 ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca
     1441 ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca
     1501 gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc
     1561 aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta
     1621 gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa
     1681 tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg
     1741 caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat
     1801 agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct
     1861 ggcagacgag agcgaggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg
     1921 gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat
     1981 aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga
     2041 atggttgatg agatttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg
     2101 gatagatttt tttttcattt gattgctcct ttatttttgg ttcattatct aataatgtag
     2161 cgaaacatct gtcagaagtc aaaagctagg gggaaacatt agtgaatagt tatatcaaat
     2221 atgtattaaa acgtgtcttt ttcatgatca tcacgctttg gttaattgca acaatcacct
     2281 ttttcttgat gcaattgctg ccgggcacgc catatacgaa tcaggaacgt ttgagtcctg
     2341 aaacaatcga gatgctaaat aaacaagtcg gtttggataa accagttatc gtacaatatg
     2401 ggatttatct ttctaatttg cttcaaggag attttggaat ttccttccaa tttaaaaacc
     2461 agccagtcgc acatttgtta gctggaagaa tcggaccatc cttgcagtta gggttacagg
     2521 caattatctt tggaacagtc ctagggacga ttttaggaac gatctctgcc atgaagcaaa
     2581 atacatgggc agatacatct tctacactcg tagcgatatt aggtcgttcc attccaaact
     2641 ttgtatttgc tgttttatta caatatatct ttgcaatcaa attacaagtt ttgccaatcg
     2701 caaaatggga tggctttgtg tacacaatct taccaacaat tgcgttggcc atgtcgccgc
     2761 ttgctgactc tgctcggttt atccgtacgg aaatggtgga agtactgcat agtgattatg
     2821 tggaattagc aaaagcaaaa ggattgagcc gttgggaaat cgcctttaaa catggtcttc
     2881 gaaatagttt gattccattg atgactttat taggaccgtt agctgtagca ttgatgaccg
     2941 gatcactggt ggtagaaaat atctttgcta ttcctggtat cggggaacaa ttcgttaagt
     3001 caatcacaac aaatgattat cctacgatca tggctgtaac gatcctttac tcatttatgc
     3061 tgatttttgt tattttgatc gtggatctat tgtacggtct agttgatcca agaattcgtg
     3121 tttcagaagg gagccgagga taaatggaaa tgataccaaa gcgttatgaa acagttgcag
     3181 atatccctgc ggatgaattt cttccattac aaaaaaatac cgaagaagaa cgtgaacaga
     3241 ttactgcacc ttctttgaat tttatccaag attcatggcg ccgtttaaag aaaaacaaag
     3301 ctgccgttat ttcaatggtt ttactattgg tcatcatttt gatctctgtg atcacaatct
     3361 ttgtttctcc tcatgatcca actgcccaaa acgtggctta tatcaatttg cctcctcgta
     3421 ttccgggaat caatatcgat ggattgaatg gaaaagcaat ggttggtggt gaacttgttg
     3481 ataaatatgc tcaagcaaac gtcccttcaa acgtaaattt ctttttagga acagacggtt
     3541 tgggacgtga tgtattaagc cgcttattta tgggaatgag aatctcgttg ctgatcgcat
     3601 ttattgctgc attactagat gtaacgattg gtgtagctta cgggttggtt tctggtttgt
     3661 taggcggacg tgtcgataac gtgatgcaac gttttttgga aatcttatca ggaattccaa
     3721 acctcgttgt catgattttg atgttggtcg tttttgaacc aggaattttt tcaatcgtgg
     3781 ctgctatggc catcacaaac tggataccaa tggcacgtat cgttcgtgca caaacaatga
     3841 aattaaaaga tcaagaatat gttttagctg gattgacatt aggtgaatca agaatgaaaa
     3901 ttgcattcaa acatattttg ccgaatatct ctagtgtcat tattattcaa atgatgttta
     3961 gtattccatc tgctattttc ttcgaagcct ttttaagctt tatcggttta ggtcttacgc
     4021 ctccgtcagc ttcattagga acgatgttga gcgaagggta caagacattc ctgtatctgc
     4081 cttatctatt atggattccg gcagtaactt tatcggttat catgattggc tttaacttac
     4141 ttgctgatgg attgcgtgat gcctttgatc ctaaaatgaa agagtgaaga cgatgacgaa
     4201 aattttagaa gtgaaagact tagaaatctc ttttgatacg tatgctggaa aggtacgagc
     4261 gattcgcggt gtcaattttc atttgaataa aggagaaaca ttggcgatcg ttggggaatc
     4321 tggaagtgga aaatcagtga cgaccagaag catcatgcgc ttgctgtcaa gcaacgccaa
     4381 tatagatgct ggtcaaatcc tctttaaagg taacgatatc gttggaaaat cagaaagaga
     4441 gatgcaaaaa atccgtggta aagaaatagc gatgattttc caagatccaa tgacctcttt
     4501 agatccgacg atgcccattg ggaagcaagt cgctgagtcc cttagaaagc ataataaagt
     4561 ttcaaaaaaa gagggacaaa aagctgcatt agaattgctt aaattagttg gaatacccga
     4621 agctgagaaa cgtatcaaca gttatccgca tcagttctca ggaggtcagc gtcaacggat
     4681 cgtgatcgcg attgcactgg tatgttatcc ggaaatcctg attgctgatg aacctacaac
     4741 agcgcttgat gtgactattc aagcacagat tttggagctg ctgaaagaaa ttcagacaaa
     4801 aatcgataca tcaattatct ttatcactca tgaccttggt gtagtagcga atgtcgctga
     4861 tcgtgtcgct gtcatgtatg gaggaagaat cgttgaagtt ggaacatcgg aagaaatttt
     4921 ctacaacccg caacatccct atacttgggg actgcttggt tcaatgccga cattagatag
     4981 tgcgaacgat cgattgtatg ctattcctgg ttctccaccg gatttattga acccgccaaa
     5041 aggagacgca ttctatccaa gaaatgaatt cgcgatgaaa atcgatgcag aacaagaacc
     5101 accatttttt gaactatcaa aaacacacaa ggcagcaact tggctacttg ctccgcaggc
     5161 acccaaagtg acaccgccag aagaaatcca acgtcgctgg gctatcttta aagagaaaca
     5221 aaaacatact tacggtcaag gaggaattgc tgatgccaaa taaactactt gaagttaaag
     5281 gattgaaaca gtatttcaat gtaggaaaaa agaatgaagt acatgctgta aatgacatca
     5341 gctttcacat ttatgaagga gaaacattcg gtttagttgg agaatctgga agcggaaaat
     5401 ccactactgg ccgtacgatc attcgcttga atgaacctac tgatggtgag atattattcg
     5461 acggtcaaga tataacgaag atcaaagata aaaaaggttt gacgaaattc cggcatgatg
     5521 ttcagatgat ctttcaagat ccatacgctt cgttgaatcc tcgtatgaaa gtcagagata
     5581 tcattgccga aggaattgat gttaatggac tagctaaatc atctgaagag cgtgcaaaaa
     5641 aagtcgatga tctattgaga actgttggat tgaatccaag ccacggtact cgttatcctc
     5701 atgaattttc aggtgggcaa cgccagcgta ttggtattgc acgagcattg gcagtaaaac
     5761 cgcgttttat catttgtgat gaaccaattt ctgcactaga tgtgtcgatc caagcccaag
     5821 tggtcaattt gttgcaagat ctccaaaaag aacatcaatt gacctatcta tttattgcac
     5881 atgatttgtc gatggttaaa catattagtg accgaatcgg tgtaatgcat aacggcttgt
     5941 tattagagat gggaacaagt gatgaaatct ataatcacgg tgttcatcct tatacagaaa
     6001 gtttgttatc tgctattcct ctaccagatc cagaccatga gagaaaacgc agaagaatca
     6061 aatatcaacc tgaaccagat gacggacaag tacgtcaact tcgtgaaatc gcaccagaac
     6121 attttgtcta tgcaactgag caggaagtcc catattacgc aaaaaaacta aaacgacaaa
     6181 aggaatctct tctggttgct aactaggagg gacatagatg ctaaaagctt ttaaattccg
     6241 tatgtatccg acagaagaac aaaaacagca attgattcgt acttttggat gtgctcgatt
     6301 tacctataac catttgctga aaaagcggca gaaaagttgg caacaaacag gagtagccaa
     6361 tttttcgctc acacccgcta cattaaaaaa agaataccca ttcttaaaag aagtagacag
     6421 tcttgcttta gcaaatgcac agcttaattt ggatcgggct tttcgcaatt atttcaaggg
     6481 acgtgccagc ttcccaaagc ttaaaacgaa aaaaagtatg tggcaatctt atacgaccaa
     6541 caaccaaacc cgtacgatct atctggaaaa tggctactta aagttaccaa aacaaaaaga
     6601 actaatcaag ataaatcagc atcggccagt tgaaggatcg attcgctccg ctacgatttc
     6661 tgctcgatat aatgaagaat tttatgtcgc actactttgt gatgtttcgc cagtaaaaaa
     6721 agaatcttta gctaaatgga tcgggatcgc ctatcatccg aagacattga tccaaacatc
     6781 tcgacctcta gaagtaactt taccaaaatt tcaccagaca gaagaaaagc tgcaacatgc
     6841 acagcggaaa ttgaatgtga aagtccgctc cgctcaccat agaaagatca ggttagatca
     6901 ggcaagcaat tatcagaaac aaaagagaaa agtaatggat ctttatctga aacaaaagaa
     6961 ccagcgagaa gattatcttg aacaattatc tgggaaattg gtcaaacaat atgactactt
     7021 atttgtagaa tcttttccaa aagaagaggc acatgctgat ttttctattc acgactggca
     7081 taaattaata acaaaattgc gttataagtc ccaatggtat aataaaaaat ttcttttgat
     7141 caataccgac ggagcagaag aaagcaattc cgtaagaaag agccaagtag tagaaaaaat
     7201 gggcagacat tcggttatca agggataaaa aatgaatgaa actgatagga gcgttagggt
     7261 tagccttgtt aataaaaagt cttaatgact tttgtttcta ggaatttttc ttttgaagaa
     7321 aagctcacta attatgacaa accaataaaa caatagcaga aaaagttcgt acgagctttt
     7381 tctgctattg ttaatttttt attcagaaag tttacgaata catcaaaatt ctgaaacaaa
     7441 aaacgtaact cacaaagagt tgtttttgcg ttttttcttg aatgattagg tctgaatctt
     7501 ttgctcacaa ccttattcat ggtgccccgg cttgagctga tagatttttg aaggacggcc
     7561 gattcctaca ggacgttcac cgatctcatc gaaatattgg agcatagctt tcttaaaatt
     7621 ggaatgatcg atcgttttat aatcgatccc taagaattta gcaaatactt ttcgagcttc
     7681 cgtaatcgtg aaatctttac ctaaaacttg tagaacttgc ggctcgtgtt ccattttatt
     7741 gactacacgg ttgaatgctt tcaagatgat ttgactatgg tcaaaagcca aagtatcttt
     7801 gccagaagac tctccagtga ctaaatcaag cgagatagcc acttctccgc tagataaatg
     7861 gatcttgtta ctatgacgtt ctagtgtgaa ccagcgtact tccttggcat catctccggc
     7921 gattagcggt tcttctccaa taaaggctaa gtagctgact gtgacgaccc agcctcgcgg
     7981 atctcgatta ggcgtactga atgtatgcag ttgctcaata ttttcttttg aaataaccac
     8041 accagtttct tcctttgttt ctcttaagac gctttctcca gttgactcat ccttttggac
     8101 aaagccacca ggcaatgccc atgaatttcg ataaggatgc ccttttcttt ggatcaacaa
     8161 aactttcagc tgatcttctt ctttgttata gcacatcagt acgatatcga cagtcaaaga
     8221 gggcttgtca tattctggac gttcctgttt ttgataccag ttaagaaatt cttcttcact
     8281 tgctttatgt tcataatata atttttcttc ttctttagaa gcaaaacggt tcatctgacc
     8341 acctccattg acagtaaaag tactttttac cttattgtag cagactctga aaaaaaacca
     8401 aggatttact tgaaaaatct aggttatcat ttaaatatga aaaataaatg gaagtatgag
     8461 attgaataaa aagaaaagca gataaaacgg caatacttta taccgctctt cacgaatggc
     8521 aaagaagcca caaataacga tatatacgtc aatgataaaa cgcaaaatcc aagctgctgt
     8581 cataaaactg ttcaaatgcc catggaggtt aaaataacca tacatacgaa tataaaaata
     8641 gctgaatatt aaatgtgcta atgaaagaaa agcaatacta agtgtaaaaa aaagatgttt
     8701 cttctgcatg attgttccag tcccttacta agatagatag tctttattat accacgtgtg
     8761 aggggagaaa taaaaagccg ttttttagta gatatgaaac aaaacaaaaa cccgcattta
     8821 ttcaacctat tcatcctttt aaaggataga ttgaacgtag atagaagtcg tttatccagt
     8881 ctttttcatt ttgaaaaaaa gctaacagca tgcttttttt atggtatact agcaaggaaa
     8941 cgaattcgca tcatgcgata aaggaggaag agccccgtgg cagaaaagga aactttttat
     9001 atcactacgc caatctatta tccaagcggc aaacttcata tcggcaactc gtatactacg
     9061 attgcatgtg acgcaattgc tcgttataaa cggttgatgg gctttgatgt gttttatctc
     9121 actggagtgg atgaacatgg tcaaaaaatc gaaacaaaag cagaagaact aggtgtaaaa
     9181 ccgcaagaat atgtagataa aatggcggct gatgtcaaga aattatggaa aacgcttgat
     9241 atcagttatg ataaattcat tcgcacgaca gatgattacc ataaagcagc tgttcaaaaa
     9301 atatttgatc gtttattaga acagggagat atttacttag gtgaatatga aggctggtac
     9361 tctgtttctg acgaagaatt ctttacagaa acacaacttg cagaagtgta caaagatgac
     9421 gaaggtaacg tgatcggtgg taaagcgcca agtggacacg aagtagaatt ggtaaaagaa
     9481 gaatcatact tcttccgcat gagcaaatac gcagaccgtc tattggaata ctatgaagaa
     9541 catcccgaat tcatccagcc agaatctcgt aaaaacgaaa tgatcaacaa ctttatcaaa
     9601 ccaggattag aggatcttgc agtttcacga acaactttta cttggggagt accagtcaag
     9661 agtgatccga aacatgtggt gtatgtatgg atcgatgcat tatccaatta tattactgca
     9721 ctaggatatg gctccgaaga tgaaagtcta ttcgaaaaat actggcctgc agatgttcag
     9781 atggtcggta aagaaatcgt tcgctttcat acgatctatt ggccaatcat gctgatggcc
     9841 cttgatttgc ctctacctaa aaaagtattt gggcatggct ggctattgat gaaagacgga
     9901 aaaatgtcta aatcaaaagg aaatgtcgtt tatcctgaaa tgcttgtgga acgttatggt
     9961 ttagatgcac tccgttatta tttgcttcgt gctgttccgt ttgggtcaga tggtgtcttt
    10021 acgccagaag attttgtttc ccgtgtcaat tatgatctgg caaatgatct agggaactta
    10081 ttgaatcgaa cgattgctat gatcaacaaa tattgtgatg gactcgttcc tgactatgcc
    10141 tcacttgtca ctccatttga tagtgaactg tctacgacgg ctgctaacgt tgtcggacgt
    10201 taccatgatg caatggaaaa aatggaattc aatacagcat tggctgaaat ctgggtgctg
    10261 atttctcgtg caaacaaata cattgatgaa acagaaccat gggtattagc aaaagacgaa
    10321 gaaaagaaag ctgaattgaa tagtgtcatg atccatttag ctgaatcttt gcgtatcgca
    10381 gcgatattac ttcagccaat catgacagaa acgccagaga agatcttcaa tcaactagga
    10441 ttggactctg aaacaatgaa tcttgaagga ttacactttg gcgaatttcc ttcaggaaca
    10501 aaagtagttg caaaaggtac accaatcttc ccacgtttag atatggaaga agaagttgct
    10561 tttattcaag aaaaaatgtc tgaaggtact caaacaaacg aagatacagt caaatgggat
    10621 ccagaagaaa cagaacttgt ttcaacgaaa gaaaaacaaa tcaaatttga tgtatttgaa
    10681 aaagtagaac taaaagtagc agaagtcatc aactgtcaaa aagtagaagg ggcagataag
    10741 ttgctgcaat tccgactaga tgctggagac agccaagacc gtcaaatctt gtcaggaatc
    10801 gctgaatttt atcctgatcc aagcgaattg atcgggaaaa aagtcgtcat tgtagcaaat
    10861 ctgaaaccac gaaagatgcg tggacaaatc agtcaaggaa tgatcctttc tgcagaagca
    10921 ccagatggct ctttacaagt aatcgaagca ccaaaatcca tgccaaatgg ctcagaaatc
    10981 gcatagaaat tcaaaggggg tatgacacta gttgtgtcat accccctttt tccgaaattt
    11041 gagaagcaat tttcgtaaat tccttcttat tacttgaagc caatcatata atacagaaac
    11101 agagaactat ccaaaataaa tcctagaaaa agaagtacga gcttcgctga tttttgttaa
    11161 tttagaataa tactaatagg aatcgtaaaa acagaaaaaa agcataatga taaagggtcg
    11221 ggcagatttt aattgattca tctggtactc ttttttattg tccttacatt cataaagttt
    11281 gaatgcaatg actttattac atctatattt atatctaaca gtagcgttga tgtgttaatt
    11341 ataagttcta tcactttatg ttcaattagt ttgtgctctt atatgtgtga ttgaaaatat
    11401 agataaaaaa acaatcatcg gctttgactt cgatgattgt tttctttatt ttgtaaaaat
    11461 cctatcactt gattttatgg taacctttta tttttgtctt caaacggctt ggtaagagag
    11521 cagtaagcag tgcaaatggg atcgaaaaga cataccagaa aatcgtacgt ccgataccat
    11581 aatgttgata gagctttgtc aacatcacgt gtaacaagat caaccctaaa agcaaggagc
    11641 cagctataaa ccaatgatta tcccaatgca tgacttcact aagtgccatc actccaaaag
    11701 gatagaaata aaaatagatc ccgactaagg tcatccgcca gttctttgta aatgttccga
    11761 tattcaataa aaagaaaatc gctgaaagca aaataccgat aggcggaaaa agcccaacca
    11821 aaaaggaata caacatcccc cacaagatca tcacaggacc tttgatcaat gcaacgattg
    11881 cgatgaacaa agtcagcaat attagttttt gtgataacgt tgcaacagtc attaatccaa
    11941 ataaaattaa tgtgaaccaa tgccaatggg actgctgttg ttctttacgg gaaaaatcga
    12001 cccggttgga taaataatct gctaatttcg gataagaacg cttaaaatcc ataaactgtt
    12061 cctccttcgt ggaaaaggta ggtttagtat acgaaaaata aaaaagaaat acatccgtct
    12121 taagagggag tttttcactg aaagtggaga aaaaagaatg gtaaattaaa atattctcag
    12181 ttatactgaa aattatacca aatttcaaag aagaaagagg atgggaatga aaggcaaact
    12241 aaaacgagaa atcaatctgt ttggcgcatt agctaccgtt atgggaaccg tgatcggtgc
    12301 aggcgtcttc ttcaaaactg ctgctgttac tgctagtact cagtctacaa gtttgacatt
    12361 gctagcttgg cttttgggtg gtttcttaac gatctgtgct ggattgacag tggctgaatt
    12421 agctacagca atcccggaaa ctggtggagc tgtaaaatat attgaagctg cgtatggaaa
    12481 attgcccagc tttttattag gatgggcgca aagcttgatt tactttcctg ctaatattgc
    12541 tgcattatcg attatctttg cgacacaatt gacgaactta ttacaattat ctaccgatta
    12601 tcttttgttg attgcagtga tcaccgctgt ttctgtgaca gggttgaatt tgttagggac
    12661 aaaagtcggt gcttctgtac aatcagtgac gttgattgtt aaattaatac caattgctgt
    12721 gattgtcata tggggattat tgaccccagg acaaggaact gtccagttat ttccaatcga
    12781 agctggaaaa gatgttactt ttgtcgaagg attgagcagt gcgttgttag caacgttgtt
    12841 tgcttatgac ggctggttag gtgtcggtgc aatggcaggg gaaatgaaac ggccggaaaa
    12901 agatttgcct aaagcaatta ttcttggttt gagttttgta acagtcgttt atctattgat
    12961 caattttgtt tttttgaaaa ctttgccgat tgatcatctt gccggtaatt tgaatgcagc
    13021 ttctgaagca tcagacgtca tttttggagg aatcggtgga aagctggtca ctattgggat
    13081 cttgatctcg gtatacggtg cattgaacgg ttatacacta actgggatcc gcgtgcctta
    13141 tgcgatggct ttagaagacg agctaccttt cagtaaacag ctgacaaagc tttcaaagaa
    13201 gtttacggtt ccttatgttc ctgcggtttt ccagttagca gtagcttgta ttatgatgag
    13261 ccttggttct ttcgactttt taacagacat gttgattttt gtgatgtggc tattcagttt
    13321 actgattttt atcggtgtct ttattttaag aaaaaaagcg ccggaattgc cacgtccata
    13381 taaagtacca ctttatccaa tcgttccgat tattgcgatt ctcggtgcta ttttcatttt
    13441 aggtatgacg atgatgacgc aaacgaaatt agcattgatc ggtataggtg ttaccttgat
    13501 aggtatccca gtctattatc aaaaaaagaa aaaacgctca gaagagtaaa atatttcaac
    13561 aacagacaga aaaagtacga actaggtcct ctagatcttc gtgctttttc tatttcaata
    13621 aaacaagttc ttctattttg atttttgcta cttctgcaag tagtgcttat gtgctaaagt
    13681 aagaatgaaa aggagagata aacatgatat ttgattctca tacccattta aatgctgaac
    13741 agttcaatga agatattcca gaaacgattc agcgagcaca agaaatgggg gtaacgaaaa
    13801 tggcggtcgt gggcttcgat acgccaacga ttgaaaagtc tttgcaatta agccatgact
    13861 atccaaatat atacagtatt atcggctggc atccgacaga ggctggcagt tatacgaaag
    13921 aaatcgaaaa taaattacaa gaacaattga ctttaccaaa ggttgtagca ttaggggaaa
    13981 ttggcttgga ttatcattgg atggaagacc cgaaagaagt ccaagataaa gtatttcgca
    14041 gacagattgc tatcgcaaaa gaaatgaacc taccaatcag tattcatact agagaagcac
    14101 ttgaagacac gtatcaaatc ctaaaagaag aagatgttcg agatatcggt ggaatcatgc
    14161 atagtttcag tggtgattat gaatgggcaa aacgatttct tgacttaggg atgcatattt
    14221 catttagcgg tgtcgtcact ttcaaaaaag cattagacgt ccaagaggct gcagcaaatg
    14281 taccaatcga acgattactt gtggaaacag atgcccctta tctggcacca gttcctttta
    14341 gaggaaagcg aaatgaacca ggctatactc gttatacagt agaaaagatt gctgaacttc
    14401 ggaatcttcc gacagaagaa gtggctgccc aaacatggac aaatgcgcac cgattatttg
    14461 gtctaagcga acatggataa gatgaagatc caagagatta tagtcgttga aggaaaagac
    14521 gatacaagaa ggctgcggga agtagttgat gcagatacga tagaaacaat agggtctgca
    14581 atcaatgaag agatcctgat gcaaattgaa catgcccaag agactcgtgg agtgatcgtt
    14641 tttaccgatc ctgatttttc aggagaaaaa ataagaaaaa tcattatgga agctgttcct
    14701 caggcaaaac atgctttttt gccgcgcaca caagctaaac caaagaaaaa gggaagcctt
    14761 ggtgtcgagc atgcaagtga tgctgctatt ttagaagcat tgaaaaaggt cgtgacgcca
    14821 gacatttccc aaacggctgt ttctgaaatc actagacaag accttttaga ctatggttta
    14881 atagctgggg cacaagcaaa agaaaaacga gaaaaactag gtgacgagtt gcgcatcggt
    14941 tacacgaacg gcaaacaatt ggaaaaacgt ttgaagatgt ttcgtattac gccgaaagaa
    15001 ctcgcccaag caatgaaaat agtggaggaa caacatgagt gaatatcgtg aaattgcgac
    15061 accctctaga acaaaagaga ttttattgaa acatggtttt tcatttaaaa aaagtttagg
    15121 acaaaatttt ctgactgaac caaatatttt aaggaaaatc gtagaaactg cgcaaataga
    15181 tgaacatacg aacgtgatcg aagttggacc aggaatcggt gcattgacgg aacaattagc
    15241 caagcatgca aaacaagtcg tagcatttga gatcgatgat cgtttgatcc ctgtattggc
    15301 tgatacgatg caaccctatg acaatgtgac tattgtacat caagatattt taaagaccga
    15361 tttgtcaaca gctgttagag aaacatttca tgaggagctt ccgttaaaag tcgtagcgaa
    15421 tttaccttac tatattacga caccgatcat gatgcatttt cttgaatcag atttggttgt
    15481 ggatgaacta gtcgtcatga tgcaaaaaga agtagctgat cgtatctctg ctgaaccagg
    15541 aacgaaagct tatggttcat tatcgattgc tgttcaatat tatatggaag ccagtttagc
    15601 attcatcgta ccaaaaacag tatttgtccc tcagccaaat gtagattcag cgattttgaa
    15661 actgacaaga agagatatac cggcagtcga agtgacagac gaaaaagcat ttttccgatt
    15721 gacaaaagcg gcatttcagc aaagaagaaa aacgctttgg aataacttgc agcattcgta
    15781 tggcaaagat gaccagacaa aagcatggtt agcaaaaagc ttggaaacag ctggaatcga
    15841 tccaaaacgt cgtggggaga ctttgtcatt acaagaattc gccgcattaa gcaatgcgat
    15901 gtcagaaaat atgcagtaaa aaaagaggtt gtgacagaag ttgtgaaaaa gctgtcctgc
    15961 atcaagtaat aagaacagcc aaacaaaaat agttcctcat gtttttcgtt tgtttgggct
    16021 tgttaccgca gatgcaagct tttgaacacc gtttaagaaa gaggttgtgg gcctgccgtt
    16081 taactctaag tcacaaggaa caatttcaaa aaacagcttc tcatgttttt gagttagttg
    16141 gtcttattac ttgatgcaag cttttgacca ctattatgcc gaacaaggag atgtgacaca
    16201 aattttgtca catctcctat ttttgtttct tgatcaaaaa attcttatac ttgatactca
    16261 cactcatttt gtatggattt agaaaagaat atgtgtcttg acagttatta gtaagtgagt
    16321 tatactaagt cgacatttat gtgtcaagac atattaggag agagaaaaaa tgagaaaaaa
    16381 ttcagttcga aaactaacga tttctgcttt attgattggg atgggggtca ttatcccaat
    16441 ggtcatgcct aaaatcatga ttccaccagc ttcctttact ttggcgtctc atgtgccatt
    16501 atttattgcg atgtttttta caccaggagt tgcagtggct gttgcgctag gtacgacatt
    16561 tggtttcttt ttaacaacgc cggttatcat cgcattgcga gcattgtcac atcttgtgtt
    16621 tgcgttgatt ggggcttggt acttgcaaaa gcatccgagt atcgttttaa aaaatggaca
    16681 gtttactttt gctaattggc gtttccaagg gttcaatttt gtgatcggtg tgattcattc
    16741 tttagcagaa atgctagtag tcagcatctt ttactttatt ggtaatatgc ctgaaacata
    16801 ttattctcaa ggatattttt atacagtatt catcttgatg gggattggtg ggcttatcca
    16861 cagtttagtt gattataata ttgcttattt tatagcagga acactcagca aatattttga
    16921 tatccccgta tttacggcag caaaaaaaga aaatcggaaa gagtcgattc aagggtacgc
    16981 gaagaaaacg acatattaga catgatctct caaaaaaaaa actctcatct agtaatggat
    17041 aataaatcct ctaagatgag agttttttgt cctaaaaaga gctgcaccaa catcttctgg
    17101 cacagctcta acagggggca atttatggaa gtttgatgac ttgttctgtt tctttatcaa
    17161 agaagtggcc tttgctgatg ttgaaggcaa ggtcgactat ttcgcctggt ttatggaaat
    17221 ctcttgcatc tacttttgag atgaactcag tagaaccagt ttttgtgtaa agcatcgttt
    17281 cagctccaag taattcggaa acgacaactt ctgatttcac tacggcttca ggtgaagctt
    17341 caagggctac ttgttcactg tggatgtctt ctggacgaat accgaagata acttcttttc
    17401 cttcataacc tttttcaact aatactttgt tttttccttc aggaatacga agcttaagcc
    17461 catgtccatc tgtgataaca ccatttttca atgtcacatt aaagaagttc atggcaggag
    17521 atccaataaa gccggcaacg aacatattga cgggtgtatc ataaacttct ttcggtgtac
    17581 cgatttgttg gataaatccg tctttcatga taacgattcg gtcagccata gtcattgctt
    17641 ctgtctgatc gtgagtaaca tagatggtcg ttgtttcaag acgctggtga agtttggcaa
    17701 tctcagcacg catggctaca cgtaatttag catctaaatt tgataatggc tcatccatta
    17761 agaagacttt tgcatcccga acgattgccc gtcccaatgc cacacgttgg cgttgtccac
    17821 cagataaagc agctggtttt cgtttcaagt attctgtcag acctaagatc tctgctgcat
    17881 tgtccacacg ttttttgatg tctgctttat catatttacg taatttcaag ccaaaagcca
    17941 tattatcaaa aacagtcata tgaggataca atgcgtagtt ttggaaaacc atggcaatat
    18001 cacggtcttt aggtgccacg tcattcatga cttttccgcc gatatttagt tctccttcag
    18061 agatatcttc cagtcccgcg atcattcgca aagtagttga ctttccacaa ccggaaggac
    18121 caacaaaaac gataaattca cggtctttga ttgatagatt aaaatcagtt acagaataat
    18181 tttctgcatt gtcatatttt ttatatacgt ttttcagagc catttctacc ataatacgtt
    18241 ccacactttc ttttttagtt ttattaacta tttaaagtat aaatgaaagc gttaactaac
    18301 ttcaatgtca tgttgaacaa gaattttttc ttttttttgt caatgtgcta gttttaagct
    18361 ttaataaata tccataagaa ataaaaactg atacactgga gtaaataagt atgttaacaa
    18421 tatcaaaagg agtgtctctt atgaaaatcg ctttgatcgc tcatgaccga aaaaaaccta
    18481 tgatggtgaa attagcaaca gcgtataaaa caatattgga aaaacatgaa ctttttgcta
    18541 ccgggacgac tgggcagagg atcacggaag caaccggctt gccgatccat cggtttaaat
    18601 ccggaccgtt aggcggggat cagcaaattg gagccatgat ttcggaggat cagttagatt
    18661 tggtgatttt tcttcgagac ccgctggctg cacagcccca tgaaccagat gtaacagcac
    18721 ttatccgttt atctgatgtc tatgagattc cgttagctac gaatatcgga acagctgagg
    18781 tgctactaag aggactggaa gccggcttcc tagattttcg aagaatcgtc catgaaatag
    18841 acaagcatcc actttcattt tgatggataa gcgtatggaa aaataacgtt taatgttcgc
    18901 ctatatgtag ctataaaagc taaacaaact gataatatgc gatataatac gaacgtattg
    18961 gataatataa acaataatat tagtttttcg tatttgcagt aaatgtgaaa atatgctata
    19021 gtttctgtgg gaaaagatcc caaaaataat acagagtaga aaataaagcg ttaagtgcta
    19081 agaggatggg atgttgcctc ttggacgaag gacgtaatgt tcgcggtttt attttcgcat
    19141 tcgctgcatg atacgatgta gcagctctta aaggagatgc taaaaatgaa gaaacaccgg
    19201 ttagacgcac gtatgattgc tattatgggc ttattgattg cattgatggt cactttgtcc
    19261 cgactggtag caatcgagac cccatttatt aaaatcagtg tcacatttat tccacaagtg
    19321 atcatgggca ttctttttgg tcctttttgg tcagggatag gcgcagtttt agctgattta
    19381 gtaggaatgg ccctcttctc gaaatctgca ttctttattg gatttacatt gaatgcattt
    19441 attgaaggag caatctacgg attctttttc tatcgaaaag agatcacttg gaaaaatgcc
    19501 attctggcta cgcttagtgt cacgctgatc atcaacttat ttttgacacc attatggctt
    19561 gcgttgatgt accatgtgcc attgttcagc tgggtcgttt gggcgccccg cctgttaaaa
    19621 acagtaatat ggttaccgat tcaatcaatt gccatttatt acgtaggacg tagtattcct
    19681 tacaaaaaaa ttttaagaag tttagcgatc cacgctaaat aaaaaacaag cagtcttccc
    19741 atcccagaag actgcttgtt tttattgtgc tgtgaattgt gcttgataga gccgtcggta
    19801 ataaccatct ttttgaagca gctcatcatg ggtgcccatt tcgacaatag ctccctgatc
    19861 catcactaat atctggtccg catttttgat tgtggacagg cgatgggcaa tcacaaaact
    19921 ggtttttcct tccatcattg ctaagaaggc ttgttggatt tttttctctg tcaatgtatc
    19981 gactgagctg gtagcttcat ctaagatcaa cataggggga ttactgatca tcgttcgagc
    20041 aatcgtcaac agttggcgtt gtccatctga tatctttaac ccttgaccgc caatcttagt
    20101 atctaatttt tccggcaaac gttctacaaa ttcatacata taagaggctt ttagggcatc
    20161 attgatttct gcgtcacttg cttccggatt cccaaaacga agattttctc gcaatgtact
    20221 gtcaaatagc caagtatctt gtaacaccat tccaaaactt ttacgtaatg tatcacgttg
    20281 gatagagcgg atgtcatggt catcgactgt aattgttcct tttgttgtgt cataaaaacg
    20341 catcagcaaa ttaacaagtg tggattttcc ggcccctgtt tttcctacta tagcaatggt
    20401 ttgacctgga taagcagtaa aattgaaatc ttcgatcaaa cgctgcttgg ggtcataaga
    20461 aaaagaaaca tgatcaaaag agacttttcc tttgatattt ttgggttcca aggcataggc
    20521 atctggcaaa tcaggctttt cttctggctg atcgatcaaa tcaaatgctc gttctagacc
    20581 agaaaaagcg gtttgcagtt gtgtcgtgat tccagaaagt tcgataaatg gtttagaaaa
    20641 ctgacttgag tagatcgtaa agctggaaat cacaccaaca gtgattgacg aaccactctt
    20701 caatgccaat aggccgccaa ctaaaccaat cgataaataa gcaaggtgat ctacaaaacg
    20761 agaagacggg tttgtcaagg aagaagaaaa ttgtgcccgt tgtccttttt catatagatc
    20821 agcatttagc agttcaaatt gctgttgact agcttcttct tgctgaaaag ctttcacgat
    20881 tttctggtta ccgattcgtt cggtcacaaa gcccgagata tccccaataa tcctttgctg
    20941 tctatcgaaa tttgtttgtg aagcacgagc aacgatccaa ctcacaagga aaataattgg
    21001 tgtagaacaa agaacgacca atgtcagaag tggacttaga cgcatcataa aaataaacga
    21061 aagaatgatg attgccactc cagaaaataa ttgattgaag gccgcagaaa ttgcaatgga
    21121 aatattgtcc atatcatttg taaatcgact gacgatattt ccgtgggagt tttgatcata
    21181 ataacgaagt ggcaatcgat tcagatgagc aaatgcatct tttcttaatt ttgcaacgga
    21241 taagtaagct aaccgattgc ttaacacttg gatgagccat tgactgacta cgttgattaa
    21301 aacgatacca ccgaatagaa taagtaaatg gaacagtcga ttgaagcgga cctgtccttc
    21361 tccaatcatt tgatcgatac tttgaccaat ttgtaacgtc atcacaactg ttgcaatccc
    21421 attgatgatt cctagtatca cagctagcca aatgtctttt gcataggcag taaggtaagg
    21481 aatgaatcgg cggagcgtct gcaaggataa tttttttact ttcataacta ttcttcctcc
    21541 tgtgatgcga cgatttcctg atattctgct gattgagcaa gtaattcttc atgggtaccc
    21601 tgagcagcaa tcttaccttg atccatcact aagatatgat gtgcttcttg aatagagcgg
    21661 acacgttggg agataataat cactgtactg ccaagataag tctgtagcgc cttacgcaaa
    21721 tccaagtctg tttgataatc caacgcactt aaagagtcat ctaaaatcag taatgctggc
    21781 ttggcaatca aagctcttgc aatcgtcagt cgctgttttt gtccgccaga gaaatttttc
    21841 ccattttcca tgacttttgt atctaaacca tttggaagct gacggacaaa ttcttctgcc
    21901 tgagcgattt tcagagcttc ccaacattct ttttctgtgg cgtctttctt accccattga
    21961 aggttttcac gaatagtccc ggtaaatagt acagaagttt ggggaacaag agcaatttgc
    22021 tgacggagat gatccagctg ccaagaacga acatccattc cgtccattat aactgtaccg
    22081 gaactgacat catagaaacg cggaatcaat tgtgtcaaag tcgttttccc gcttccggtt
    22141 ggaccaatga ttccaaggac tgtatttcgc ttcagctgaa atgtaatatt ttggagagaa
    22201 agaccagaat caggagtata acggaagtca acatggtcaa aagcgataat tgcttcagaa
    22261 gaaaaatcgg gagtagtact gacctcagct tcaagaatcg aattttctgt gtctagcacc
    22321 tctttcaccc gatttcctga agcttcggca cgtgtgaaga tcacgacgag gtttgaaaca
    22381 acgatcagtg ccaaaagcat ttgattcata taattgatca aagcaagaac ttgtccttgt
    22441 tgcaagctac caatatttac tttgatcccg ccaactgtca gcaaacagat gatcccgaca
    22501 ttcatgatca aggttgtagc aggtgataat agggcagaga tattggaaac acgtaaataa
    22561 ttgttcgcta gatcatttgt gcttttatcg aacttctctg tttctttttt tgtctgcgca
    22621 aatgcgcgga tgacacgaac accgctaaga ttctgactaa cgtgttcatt gagctgatct
    22681 agtttttctt gcactttttg gtagagagga acggtttttt tgatgataaa aaataaaatg
    22741 attgagaaaa taggcaataa tgctaagaaa aacagaccta cttcccagtc gatcacaaaa
    22801 gccatgacca ctgacccgat acttaaaaac ggtgctctga tcaataaacg aatgaccatt
    22861 gctaaagcta gctgaagctg attgatgtcg tttgtcattc gggtgatcaa ggtatctgtt
    22921 ccaaaatgat tcaactcgtt gtgagagaaa gtattgattt ttttgattag ctgattacgt
    22981 aactctgtac caaatccttg tgaagctact gaagcatagt attggcaaat gatagcacaa
    23041 atcagaccaa tcacagacat agcgaccatc cataagccca tttcaatgat ttttcctcgg
    23101 tcattcattt ttagtccatt atcgatcaag gaagccatca ttaaaggcag gacaagttcg
    23161 aagacagctt caagaaattt gaagaacggg cccaaaatga tttgcttacg atagtctttt
    23221 gcataacgta ataactgaat catatgattc ctcctattat ttatttgtcc acaattgaaa
    23281 aaacaagtac taccacattg tagcttagga aatttagaat gaaaagagaa caagcctttt
    23341 cataaatgaa agttttatta tgaagaagct ttagaaattg aatagactat tttttttaaa
    23401 aaaa
//
LOCUS       DAPCMU010000030        23060 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPCMU010000030 DAPCMU010000000
VERSION     DAPCMU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.030"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(111..566)
                     /locus_tag="RD744_000978"
     CDS             complement(111..566)
                     /locus_tag="RD744_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0556998.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="RD744_000979"
     CDS             complement(720..1928)
                     /locus_tag="RD744_000979"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDU0556999.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="RD744_000980"
     CDS             complement(2040..2372)
                     /locus_tag="RD744_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDU0557000.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="RD744_000981"
     CDS             2520..3395
                     /locus_tag="RD744_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0557001.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="RD744_000982"
     CDS             complement(3477..4058)
                     /locus_tag="RD744_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDU0557002.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="RD744_000983"
     CDS             complement(4064..5323)
                     /locus_tag="RD744_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDU0557003.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="RD744_000984"
     CDS             complement(5325..6371)
                     /locus_tag="RD744_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557004.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="RD744_000985"
     CDS             complement(6606..6881)
                     /locus_tag="RD744_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDU0557005.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="RD744_000986"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="RD744_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDU0557006.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="RD744_000987"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="RD744_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDU0557007.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9983..10660)
                     /locus_tag="RD744_000988"
                     /pseudo
     CDS             complement(9983..10660)
                     /locus_tag="RD744_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
     gene            complement(10709..11344)
                     /locus_tag="RD744_000989"
     CDS             complement(10709..11344)
                     /locus_tag="RD744_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0557008.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="RD744_000990"
     CDS             complement(11410..12813)
                     /locus_tag="RD744_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDU0557009.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="RD744_000991"
     CDS             complement(12800..13831)
                     /locus_tag="RD744_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557010.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="RD744_000992"
     CDS             13862..14083
                     /locus_tag="RD744_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDU0557011.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="RD744_000993"
     CDS             complement(14248..14985)
                     /locus_tag="RD744_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDU0557012.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="RD744_000994"
     CDS             complement(15138..16478)
                     /locus_tag="RD744_000994"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDU0557013.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="RD744_000995"
     CDS             complement(16715..18238)
                     /locus_tag="RD744_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDU0557014.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="RD744_000996"
     CDS             complement(18616..19227)
                     /locus_tag="RD744_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0557015.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="RD744_000997"
     CDS             complement(19557..20273)
                     /locus_tag="RD744_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDU0557016.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="RD744_000998"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="RD744_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDU0557017.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="RD744_000999"
     CDS             complement(20832..21629)
                     /locus_tag="RD744_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDU0557018.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="RD744_001000"
     CDS             complement(21599..21970)
                     /locus_tag="RD744_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDU0557019.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="RD744_001001"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="RD744_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDU0557020.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6466 a         4926 c         3910 g         7758 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt aaaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta
//
LOCUS       DAPCMU010000031        22851 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPCMU010000031 DAPCMU010000000
VERSION     DAPCMU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.031"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     regulatory      180..259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            257..1450
                     /locus_tag="RD744_001002"
     CDS             257..1450
                     /locus_tag="RD744_001002"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDU0557021.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1580..3052
                     /locus_tag="RD744_001003"
     CDS             1580..3052
                     /locus_tag="RD744_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0557022.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3135..3923
                     /locus_tag="RD744_001004"
     CDS             3135..3923
                     /locus_tag="RD744_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDU0557023.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3990..4658)
                     /locus_tag="RD744_001005"
     CDS             complement(3990..4658)
                     /locus_tag="RD744_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDU0557024.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4773..6485
                     /locus_tag="RD744_001006"
     CDS             4773..6485
                     /locus_tag="RD744_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557025.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6488..8257
                     /locus_tag="RD744_001007"
     CDS             6488..8257
                     /locus_tag="RD744_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557026.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8462..9028)
                     /locus_tag="RD744_001008"
     CDS             complement(8462..9028)
                     /locus_tag="RD744_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0557027.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9028..9984)
                     /locus_tag="RD744_001009"
     CDS             complement(9028..9984)
                     /locus_tag="RD744_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDU0557028.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9984..10640)
                     /locus_tag="RD744_001010"
     CDS             complement(9984..10640)
                     /locus_tag="RD744_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDU0557029.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11010..13028
                     /locus_tag="RD744_001011"
     CDS             11010..13028
                     /locus_tag="RD744_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0557030.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13098..13304
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13455..15869
                     /locus_tag="RD744_001012"
     CDS             13455..15869
                     /locus_tag="RD744_001012"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDU0557031.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16217..17770
                     /locus_tag="RD744_001013"
     CDS             16217..17770
                     /locus_tag="RD744_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDU0557032.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18151..19935)
                     /locus_tag="RD744_001014"
     CDS             complement(18151..19935)
                     /locus_tag="RD744_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557033.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19950..21671)
                     /locus_tag="RD744_001015"
     CDS             complement(19950..21671)
                     /locus_tag="RD744_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557034.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21771..22532
                     /locus_tag="RD744_001016"
     CDS             21771..22532
                     /locus_tag="RD744_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0557035.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7265 a         4084 c         4724 g         6778 t
ORIGIN      
        1 ctttttgagt atctttttct ttttatctga aagaatgatt ttaaaaagct tagcagagtt
       61 atctttatgg atgattgtgc taagcttttt tggacagttc taatgtaaaa tatttttata
      121 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
      181 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      241 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      301 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      361 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      421 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      481 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      541 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      601 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      661 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      721 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      781 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      841 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      901 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      961 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
     1021 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
     1081 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1141 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1201 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1261 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1321 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1381 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1441 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1501 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1561 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1621 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1681 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1741 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1801 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1861 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1921 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1981 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     2041 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     2101 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2161 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2221 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2281 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2341 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2401 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2461 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2521 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2581 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2641 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2701 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2761 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2821 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2881 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2941 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     3001 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     3061 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3121 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3181 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3241 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3301 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3361 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3421 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3481 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3541 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3601 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3661 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3721 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3781 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3841 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3901 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3961 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     4021 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     4081 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4141 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4201 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4261 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4321 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4381 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4441 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tatttgcaag
     4501 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4561 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4621 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4681 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4741 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4801 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4861 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4921 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4981 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     5041 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     5101 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5161 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5221 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5281 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5341 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5401 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5461 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5521 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5581 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5641 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5701 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5761 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5821 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5881 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5941 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     6001 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     6061 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6121 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6181 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6241 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6301 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6361 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6421 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6481 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6541 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6601 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6661 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6721 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6781 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6841 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6901 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6961 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     7021 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     7081 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7141 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7201 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7261 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7321 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7381 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7441 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7501 aaactgtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7561 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7621 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7681 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7741 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7801 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7861 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7921 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7981 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     8041 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     8101 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8161 tgatggggaa gtcattgaag aaggcagtca tcacgagtta ttagcagaaa atggatttta
     8221 tgcggaactt tatcataatc aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct
     8281 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga
     8341 cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc
     8401 tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta
     8461 tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc
     8521 gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat
     8581 ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag
     8641 gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga
     8701 ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt
     8761 gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt
     8821 ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa
     8881 aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat
     8941 tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat
     9001 ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt
     9061 tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt
     9121 tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg
     9181 gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg
     9241 acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat
     9301 cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac
     9361 attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac
     9421 acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc
     9481 tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa
     9541 atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg
     9601 tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc
     9661 tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg
     9721 ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag
     9781 agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc
     9841 gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt
     9901 gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt
     9961 tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt
    10021 tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtaa
    10081 ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt
    10141 actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa
    10201 gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta
    10261 gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa
    10321 aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa
    10381 aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg
    10441 atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga
    10501 taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg
    10561 aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga
    10621 aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt
    10681 tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc tttgaataac
    10741 caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta atgcaaatga
    10801 aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa cgtagacgta
    10861 ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata tgattacaaa
    10921 aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata attattaata
    10981 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
    11041 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
    11101 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
    11161 aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta
    11221 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
    11281 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
    11341 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
    11401 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
    11461 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
    11521 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
    11581 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
    11641 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
    11701 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
    11761 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
    11821 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
    11881 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
    11941 ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg
    12001 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
    12061 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
    12121 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
    12181 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
    12241 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
    12301 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
    12361 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
    12421 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
    12481 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
    12541 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
    12601 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
    12661 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
    12721 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
    12781 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
    12841 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
    12901 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
    12961 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
    13021 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
    13081 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
    13141 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
    13201 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
    13261 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
    13321 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
    13381 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
    13441 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
    13501 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
    13561 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
    13621 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
    13681 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
    13741 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
    13801 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
    13861 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
    13921 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
    13981 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
    14041 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
    14101 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
    14161 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
    14221 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
    14281 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
    14341 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
    14401 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
    14461 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
    14521 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
    14581 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
    14641 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
    14701 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
    14761 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
    14821 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
    14881 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
    14941 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
    15001 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
    15061 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
    15121 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
    15181 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
    15241 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
    15301 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
    15361 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
    15421 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
    15481 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
    15541 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
    15601 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
    15661 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
    15721 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
    15781 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
    15841 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
    15901 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
    15961 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
    16021 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
    16081 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
    16141 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
    16201 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
    16261 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
    16321 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
    16381 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
    16441 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
    16501 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
    16561 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
    16621 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
    16681 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
    16741 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
    16801 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
    16861 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
    16921 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
    16981 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
    17041 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
    17101 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
    17161 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
    17221 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
    17281 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
    17341 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
    17401 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
    17461 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
    17521 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
    17581 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
    17641 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
    17701 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
    17761 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
    17821 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
    17881 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
    17941 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
    18001 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
    18061 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
    18121 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
    18181 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
    18241 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
    18301 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
    18361 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
    18421 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
    18481 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
    18541 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
    18601 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
    18661 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
    18721 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
    18781 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
    18841 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
    18901 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
    18961 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
    19021 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
    19081 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
    19141 ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt
    19201 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
    19261 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
    19321 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
    19381 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
    19441 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
    19501 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
    19561 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
    19621 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
    19681 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
    19741 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
    19801 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
    19861 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
    19921 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
    19981 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
    20041 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
    20101 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
    20161 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
    20221 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
    20281 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
    20341 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
    20401 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
    20461 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
    20521 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
    20581 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
    20641 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
    20701 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
    20761 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
    20821 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
    20881 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
    20941 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    21001 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    21061 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    21121 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    21181 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    21241 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    21301 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    21361 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    21421 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    21481 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    21541 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct
    21601 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    21661 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    21721 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    21781 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    21841 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    21901 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    21961 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    22021 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    22081 gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    22141 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    22201 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    22261 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    22321 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    22381 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    22441 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    22501 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    22561 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    22621 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    22681 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    22741 aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa
    22801 aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa a
//
LOCUS       DAPCMU010000032        22639 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPCMU010000032 DAPCMU010000000
VERSION     DAPCMU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.032"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            31..843
                     /locus_tag="RD744_001017"
     CDS             31..843
                     /locus_tag="RD744_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDU0557036.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            840..1640
                     /locus_tag="RD744_001018"
     CDS             840..1640
                     /locus_tag="RD744_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDU0557037.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1801..3069
                     /gene="rseP"
                     /locus_tag="RD744_001019"
     CDS             1801..3069
                     /gene="rseP"
                     /locus_tag="RD744_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDU0557038.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3137..4846
                     /locus_tag="RD744_001020"
     CDS             3137..4846
                     /locus_tag="RD744_001020"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDU0557039.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5058..9416
                     /locus_tag="RD744_001021"
     CDS             5058..9416
                     /locus_tag="RD744_001021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDU0557040.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9561..10034
                     /gene="rimP"
                     /locus_tag="RD744_001022"
     CDS             9561..10034
                     /gene="rimP"
                     /locus_tag="RD744_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDU0557041.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10057..11232
                     /gene="nusA"
                     /locus_tag="RD744_001023"
     CDS             10057..11232
                     /gene="nusA"
                     /locus_tag="RD744_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDU0557042.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11254..11547
                     /locus_tag="RD744_001024"
     CDS             11254..11547
                     /locus_tag="RD744_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDU0557043.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11544..11855
                     /locus_tag="RD744_001025"
     CDS             11544..11855
                     /locus_tag="RD744_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDU0557044.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11868..14174
                     /gene="infB"
                     /locus_tag="RD744_001026"
     CDS             11868..14174
                     /gene="infB"
                     /locus_tag="RD744_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDU0557045.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14201..14548
                     /gene="rbfA"
                     /locus_tag="RD744_001027"
     CDS             14201..14548
                     /gene="rbfA"
                     /locus_tag="RD744_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDU0557046.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14705..14878)
                     /locus_tag="RD744_001028"
     CDS             complement(14705..14878)
                     /locus_tag="RD744_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557047.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15326..17688
                     /locus_tag="RD744_001029"
                     /pseudo
     CDS             15326..17688
                     /locus_tag="RD744_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18056..18979
                     /gene="truB"
                     /locus_tag="RD744_001030"
     CDS             18056..18979
                     /gene="truB"
                     /locus_tag="RD744_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDU0557048.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18983..19924
                     /gene="ribF"
                     /locus_tag="RD744_001031"
     CDS             18983..19924
                     /gene="ribF"
                     /locus_tag="RD744_001031"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDU0557049.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20276..21061
                     /locus_tag="RD744_001032"
     CDS             20276..21061
                     /locus_tag="RD744_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0557050.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21074..21925
                     /locus_tag="RD744_001033"
     CDS             21074..21925
                     /locus_tag="RD744_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDU0557051.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22049..22501)
                     /locus_tag="RD744_001034"
     CDS             complement(22049..22501)
                     /locus_tag="RD744_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557052.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7682 a         3954 c         4953 g         6050 t
ORIGIN      
        1 gggaatcttt taagtatagg aggaaaaaga atgttacgtt tttttccgca aaaaaacaaa
       61 tatattcaag aaaaaagcga atttcaattt aataccgatg gaccaattcc tcagcacatt
      121 gccatcatca tggacggcaa tggtcgttgg gcgcaaaacc gtcgtttgcc acgagttgct
      181 ggccataaag aaggcatgga aacagtaaag aaagtcacaa aaaaagcatc aaggcttgga
      241 gtgaaagtgt tgacgcttta tgccttttcg actgaaaact ggaagcgacc aaaagatgaa
      301 gtaagttttc tcatgcaact acctgtcgac ttctttgata cgttcgtacc agaattgatt
      361 aaagaaaatg tcaaagttca tgtcatgggt tatgaaaacg tattgccaga acatacgcaa
      421 gatgctgtaa gacgtgcaat tgagcaaaca aaaaacaata ctggaatggt attgaatttt
      481 gctttgaatt atggaagcag agcagagatc gtgactgctg tccaagaaat tgccgaagaa
      541 gtagcaaaag gagaaataca cgcagaagaa atcgatgatg aattgattgc taagcattta
      601 atgacaggtt tcttgcctaa agagttacaa gatcctgagc tgatgatccg tacaagtgga
      661 gaagagcgaa ttagtaattt tctgttatgg cagatcgcat atagtgagct gtattttaca
      721 aaagcgcttt ggcctgattt tgatggtgcg catcttgaag aagcaatcgc ctcttatcaa
      781 aatcgagacc gtcgattcgg cggtgtaaaa aaaacagaag atgagaaagg ggatcagtca
      841 tgaaacaacg tgtggtcaca gcagccgtgg cgctagtctt gtttattccg attatatgga
      901 tcggaggcat agcggttgag cttacagcag caatcttagc agtaatagga gtttacgagc
      961 tgtttcggat gaaaggattg acactcttaa gttttgaagg agttttatcc gccgttggtg
     1021 cagtgttact tgttttgcca aaagaaagat ggtttttctt tcttccagag aatgtaagta
     1081 catttatgtt attttatctt atagttatga ttcttttagg aacatcagtt atttctaaaa
     1141 atacgtatac gattgatgaa gcaggttttc ctgtgcttgt cagcttgtat gtgggaatag
     1201 gtttccagaa ttttgtcaat gcacgaacag aaagcttggc tgttttgata tttggtctat
     1261 ttattgtttg ggcaactgat atcggagcat atatggttgg tcgacgttat ggtcagcata
     1321 agttaagtcc tgatatttcg cccaacaaaa cgattgaagg tgcgctcggt ggtattggta
     1381 gtgctgttgt agtcgcattt ctttatttat tgctataccc agcaaaagaa ctatttaatc
     1441 atggtatgtt tgtcatgctt ctgatgacga ttattttttc agcagtcgga caatttggtg
     1501 atttggtcga atctgcaatc aaacgtcatt atgatgtgaa agactcggga aatatcttac
     1561 caggccatgg tggaatctta gaccgtttcg acagtttgct gtttgtattt cctatcatgc
     1621 atttatttgg cattatttag taagtatacc tgcgtttctt tgtgaatttc ataaaagacg
     1681 caggttttta tttttcatta tattgatact attggtgtct ttggtatact atggtaaaat
     1741 tatagtgttt tatcgttgtt ttataaaaaa cacaaaaaac aagtaaagga gaaaaaagct
     1801 atgaaaacga ttctgacatt tatcatcgtt tttggtatat tagtgattgt tcatgagttt
     1861 ggtcatttct tctttgcgaa acgatcagga atcctcgttc gcgaatttgc tatcggtatg
     1921 ggtccgaaaa tttatgggca tcaagcaaaa gacgggacaa catatacgct tcgcctatta
     1981 cctattggcg gttatgttcg aatggcaggc aatggggatg atgaaacaga aatggcacca
     2041 ggaatgcctc tttctctttt actaaattct gacggcatcg tagagaagat aaatttaagt
     2101 aagaaaatcc aattgacaaa tgctatcccg atggaattga gccgttatga tttagaagat
     2161 gagctgacta tcactggtta tgtgaatgga gacgaaacag aagtagtaac ctaccctgtt
     2221 gatcacgatg cgacgattat tgaaaacgac ggaacagaaa tcagaattgc gccaaaagac
     2281 gtacagttcc aatcggctaa attgtggcag cgtatgctga ccaactttgc tggacccatg
     2341 aataacttta ttttggcaat cctgttattc atcattctgg catttatgca aggtggtgtt
     2401 caagtgacga acactaatcg cgtaggagag atcatgccta atggagctgc ggcagaagca
     2461 ggcttaaaag aaaatgatga ggtagtcagt gtggacggaa aagaaatcca ttcatggaat
     2521 gatctgacaa cagtcattac gaaaaatccg gataaaacat tagactttaa gatcgaacga
     2581 gaaggtcagg tgcaatctgt cgacgtgaca ccgaaatctg tagagtcaaa tggcgaaaaa
     2641 gttggccaac ttggtataaa agccccaatg aatacaggtt ttatggataa gatcattgga
     2701 gggacgcgtc aagctttcag cggttcattg gaaattttca aagccttagg atcactattt
     2761 acaggtttca gtttagataa attaggcgga cctgtcatga tgtaccaatt gtcttcagaa
     2821 gcagcaaatc aaggtgtaac aactgttatt agtttgatgg cgcttctttc aatgaatctc
     2881 ggaatcgtca atctgcttcc gattcctgcc ttagatggcg ggaaattagt cttgaatatt
     2941 tttgaaggga ttcgcgggaa accattaagt caagaaaaag aaggaatatt gacattagct
     3001 ggctttggat ttttaatgtt gttgatggtg ctagttacat ggaacgatat tcaacgattc
     3061 tttttctaga aaaagttgac aaatagggta taataacaaa cgataactat tttgaggaaa
     3121 taaaggagta tatagaatga gacaatcaaa aatgttgatt ccaacattac gcgaagttcc
     3181 aaatgatgca gaagtattaa gccatcaaat cttgctaaga gctggatata tccgccaagt
     3241 atcagcgggt atttattcat atctgccatt agcaaatcgt gtattggaaa aactgaaacg
     3301 cattatgcgg gaagaatttg aaaaaatcgg agcggtagaa atgctgatgc ctgccatctt
     3361 gccagcagaa ctttgggaag aatccgggcg ttacgaaaca tatggaccaa acttgtatcg
     3421 tttcaaagac cgcaatgatc gaaaaatgat ccttggacca actcacgaag aaacttttac
     3481 tgatttgatg cgtaatgaaa tcaattcata taagaaatta cctttaaatc tttatcaaat
     3541 ccaacctaaa tatcgtgacg aaaaacgacc tcgcttcggt ttgcttcgcg gaagagagtt
     3601 tatcatgaaa gatgcgtatt ctttccatgc aacggaagaa agtttggacg agacatataa
     3661 agattacgaa aaagcctaca ccgaagtatt caagcgttgt ggcttagaat ttcgctcgat
     3721 tatcggagac ggtggcgcaa tgggcgggaa agactcaaaa gaattcatgg cgatctcaga
     3781 aatcggggag gatacgatct gttattcgac tgaaagtgat tatgctgcaa acttggagat
     3841 ggctacaagc tactatgtat caaaaaaatc gcatgaaaca cagcttgaat tggagaaagt
     3901 agctacacct aacgtaaaat caattgacga agtagcagat ttcttttctg ttgaacctca
     3961 aaaaatcatc aagtctgtat tgttcattgc ggatgaacaa cctgtccttg ttttggtacg
     4021 cggagaccat gaagtcaatg acgtaaaact gaaaaacttt ttatctgctg actttttaga
     4081 agaagcaaca gaagaagaag cacaaaaata tctaggtgct gagtttggtt ctgtcggacc
     4141 agtcggtgtt tcagatgacg taaaagtata tgcagaccgt tatgtccaag atttagcgaa
     4201 tgccattact ggagcgaacg aaacaggatt ccactataaa aatgtgaatc cagaacgcga
     4261 tttcactgta ctttcttatg aagatcttcg ttttgtacaa gaaggagatc cttcaccaga
     4321 caacaatggt gttcttgcct ttactcgagg aatcgaaatc ggacatatct tcaaaatcgg
     4381 cactcgttac agtgaatcta tgggagcaac tgtactagac gaaaatggac gagaaaaatt
     4441 tgtgattatg ggctgttatg gtatcggtgt aagtcgttta ttatcagcaa tcgtagaaca
     4501 acattcagat gaacaaggaa tcaactggcc aaaaggaatt gcgccatttg atttgcatat
     4561 cgtccaaatg aatctaaaag acgaatacca aacaaacctg acgaatgaac tagaagaagc
     4621 gatgacaaaa gctggctatg aagtactggt tgatgacaga aatgaacgcg caggagtgaa
     4681 attcgcggat tctgatttga ttggctgtcc aatcagaatc actgtcggta aaaaagcagt
     4741 ggatggaatc gtagaagtga aaatcaagaa aactggcgaa atggttgaag tacgtaaaga
     4801 agaattggtg aatactttac caatcctctt gaaccaaggc gaataataag cgttaaaaca
     4861 cagaaaataa aaatagctga atcagctgtt tttgacaatc tgatgattcg cttttaaaat
     4921 tggaactgcg atttgaatcg cagttccaat tttttaatgt aaaactcaga aaaaacgagt
     4981 atactaaaag gtactgaaaa agaagaggat aagatgcgat caattgcatt gtttctcatc
     5041 agaaagggcg tatatctttg tcagaaaaac gagatttatt tgaaaaactg ttggaacaaa
     5101 tccaactaga ggaagcagaa aaaaatcatc cactgcttac tgctggtgaa atggatagtg
     5161 tagtggtcca tcgtaaatcg cgtttatggg aatttacgct gcatttttcc aaaattttgc
     5221 ctattatgct atatcggtca ttgactcagc atctgactat cgcttttaaa gatattgcac
     5281 aagtcaaact gaatatctta gcagatgacc agacattcga tgaacagctc ttgcaagatt
     5341 actgggccca agctttggaa aatcagcaat gcgatacacc actagcacag caagtattga
     5401 aaacgcaggt acctgtgata aaagatcgta aagtcatctt gccaatcgac agtacaggag
     5461 ctatttctta tttgaagcaa caatacttgc cattagtaga agagttgttc gtcagttacg
     5521 gattcccaaa atttcgtatc gaaccagaag tggatgagca gcaagcggaa cgagtattga
     5581 aactttttga agaacgcaaa caagaacagg cagaagcctt tatgaagcaa gcagctgaaa
     5641 gtctagttgt ccacgaacaa aagaaaaagg aaagaaaaga gcaatcgcca gctcttgaag
     5701 gacccatcca catccagcta ggacgaaata ttccagccga tgaaccaacg acaccaatga
     5761 tcagtatcgt tgaagaagaa cgaagagtca cacttgaggg gtacgtattc gacaaagaag
     5821 tcagagaact acgttcaaaa cgcaagatct tgactttgaa aatcacagat tatacttcat
     5881 catttatcgt caaaaaattc tcgaacggcg aaaaagatga acaagtattt gatgcgatca
     5941 gtgtcggtag ctggttaaag gtacgaggaa gtattcaaga agatacgttt gtccgtgatt
     6001 tggtcatgaa tgcgcaagat atcatagaag tcaaacatac acctcgcaaa gattatgctc
     6061 ctgaagggga aaaacgagta gagcttcatg ttcatagcaa tatgagtacg atggatgcaa
     6121 caaacagcat ctctgatctt gtggcacaag cagggaaatg gggacaccga gctatcgcca
     6181 ttacagacca cggtggtgcg caagcctttc cggaagcaca tagtgctgga aaaaaagcag
     6241 gtgtgaaaat cttatacggc gtggaagcga atgtcgtaga tgatggtgtc ccaattgctt
     6301 ataatgatgc acatgaagca ttgagtgaag ccacatatgt cgtatttgac gtggaaacga
     6361 caggattgtc tgcagtatat gacacgatca tcgagctagc cgcagtcaaa atgtataaag
     6421 gaaatgtgat cgaaagcttt gatgaattta tcgatccagg acatcctttg tcacgaacaa
     6481 cggtagatct gactggtatc acggatggaa tggttcgagg atcaaagtcg gaagaagaag
     6541 tattgcggat gttccttgaa ttttcaaaag atacaatttt ggtagcccac aacgcggctt
     6601 ttgatatggg gttcttgaat actagctacg caaggtatgg tattcctgaa gcggccaatc
     6661 cagtcattga tacgttggaa ttagctagat atctgtatcc tcaattcaaa cgtttcggat
     6721 taggtgtgtt atctaaaaaa tttggtgtca gcttggaaca gcatcaccga gcaatctatg
     6781 atgctgaagc aactggtcac ttagcatgga tctttgtgaa agaggcaatg gacaatcata
     6841 acatgcttta tcatgatcag ctaaatgagc atatcggaga aggagattct tacaaaaggg
     6901 ctcgaccttt ccatgtaacg attttagcaa aaaatcaggc aggactgaaa gatttgttca
     6961 aactgatttc aatgtcgaat gtcgaatact tcgaacgcgt cccacgaata ccacgctccc
     7021 aattgaaaaa gatgcgtgaa aatctattga tcggctcagc ttgtgataag ggagaaatat
     7081 tcgaagccat gatgcaaaaa ggggtagaag aggcaagaaa ccgagcaaaa ttctacgatt
     7141 atattgaagt tatgccaaaa gcggtctatg cgcctttgat cgagcaagaa ttggtaaaaa
     7201 atgaacatga tttggaagaa attatccaaa atttggtaga aatcggaaaa agtttggata
     7261 aaatcgttgt tgcaacaggt aatgtacatt atctgaacga agaagatgcc atttaccgaa
     7321 aaattttgat caattctatg ggcggagcga atccgttgaa tcgtcatagt ttgccggatg
     7381 tccatttccg aacaaccgat gagatgctaa ctgcatttca ctttttaggg gaagaaacag
     7441 cgaaagagat cgtagtagaa aatacgaata aaatcgcaga tatctgcgaa gaagtgattc
     7501 cagtcaaaga tgaactttat acaccgaaga ttccaggttc agaagatgaa atatcagaat
     7561 tgagttacac aaaagcaaaa caaatgtacg gagatccgtt acctgagatc attcaaaaac
     7621 gattaaaaaa agagttgaat tctattaatg gtaatggctt ctcggttatt tatctgatcg
     7681 cccaaaaact agtgcataaa agtaatgaag atggctatct agtaggttca cgtggctctg
     7741 ttggctctag ctttgttgcg acgatgacag gaatcactga agttaatccg ttagcccctc
     7801 attattattg tccagagtgc caatattccg agttttttga agatggtacg tatggttctg
     7861 gatttgacat gccagaaaaa caatgtccaa aatgtggtgc acgcttaaac aaagacggtc
     7921 atgatattcc atttgagaca tttttaggat tccatggaga caaagtgccc gatattgatt
     7981 tgaacttctc aggtgattat caagcagaag cccataacta tacgaaagta ttgtttggcg
     8041 aagactatgt ttaccgtgcc ggaacgattg gtacagtcgc cgacaaaaca gcatatggct
     8101 atgtaaaagg atacgaaagg gacaataatc tgcagtttcg aagtgcagaa gtcgacagat
     8161 tagcaaaagg cgctactgga gtcaaacgga caaccggcca gcatccaggg gggatcatcg
     8221 ttattcccga ttatatggat gtctatgatt ttacgccgat ccaatatcca gcagacgatc
     8281 aaaattcgga atggaaaacg actcattttg attttcactc gattcacgat aatgtattga
     8341 aattagatat tctagggcat gatgacccaa ctgttattcg gatgcttcaa gatttatcag
     8401 ggatcgatcc acaaacgatc cctacggatg acccggaagt gatgcggata tttgccggtc
     8461 ctgaagtact tggagtaagt caggaacaaa tttattccaa aacagggaca ttaggtattc
     8521 cagaattcgg cactcgtttt gttcgaggga tgctagaaga aacgcatcct acaacttttg
     8581 cagaactact gcaaatttcc ggactatctc atggtacaga cgtatggtta ggaaatgcgg
     8641 aagaattgat tcgacgagga gatgcgacac tggcggaagt aatcggctgt cgtgatgata
     8701 ttatggttta tttgatccat gctggattag atagcgggat ggcatttaaa atcatggaaa
     8761 ctgttcgtaa aggacaatgg aacaagatcc ctgatgaatt gcgagaaacc tatctatcgg
     8821 cgatgaaaga aaataatgtg cccgactggt atatcgattc ttgttcaaaa atcaaatata
     8881 tgtttccgaa agcccatgct gcagcttacg tattgatggc tcttcgtgtc gcttatttca
     8941 aggtttattt ccctattttg tattactgtg catacttttc tgtacgtgca gacgactttg
     9001 atctagtctc catgtgcaaa ggaaaagatg ctgtgaaaca agcaatgaaa gaaatcacgg
     9061 acaaaggatt agatgcttcc gtaaaagaaa agaatcaact gacagtgctg gagttagcca
     9121 atgaaatgct ggaacgagga ttcaaatttg gaatgattga tttgtataaa tcggatgccg
     9181 ttaactttgt tattgaaggt gatacgttga tcgcaccatt tagagctgtc ccaagtttgg
     9241 gaaccaacgt ggcaaaacaa atcgttgaag caagaaaaga cggtccattt ttatccaaag
     9301 aagatcttgc cacccgcggt aaagtttcaa aaacattgat cgaatatatg aacgacaatg
     9361 gcgtattgaa agatctacca gatgaaaatc aattatcgtt attcgatatg ctataagcat
     9421 ctgttttcct gtctgcttgt aattttaagc agagtatggt atagttctta taggtaatga
     9481 tactaactgt gagtgagcgg atttttcgct cactcttttt aatggaatta cgcacttaat
     9541 tttttaagga ggcgaaactt ttgagtagcg tcgttgaaac agtcacagaa atggtgacac
     9601 cgattttaga tgaacaaaat tttgaacttg tcgaagtcga atttgtaaaa gaaggaaaaa
     9661 gctggttctt acgagtattt attgacaaag aaggcggtat cgatatcgaa gaatgcgcct
     9721 ttgtcagtga gaaactgagt gaaaaacttg atgcagcaga tccagatccg attcctcaag
     9781 cttatttcct ggaggtatcc tccccgggag cagagcggcc attgaaaaaa gaaagcgact
     9841 atgaaaaggc acttggtgat tatgtccatg tctccttata ccaaccagta gatggcgaaa
     9901 aacaatatga aggatttctt caatcatttg atgctgaaca gcttacattg aaaatacgca
     9961 taaaaacacg agaaaaagaa ttgacttttg accgaaaaaa tattgcaaaa gctcgcttag
    10021 ctatccaatt ttaatcacgg agaggaatca gaaaaaatga gtaaagaaat gttaaatgcg
    10081 ttagacgctt tagaagctga aaaagggatc tcaaaagaaa tcgtgatcga tgctttagaa
    10141 gcagcattgg tttctgcata caaacgtcac tatggccaag cacaaaacgt cgaagtggaa
    10201 tttgatcaga aaaaaggaaa gatccatgtc tatgcggtaa aagaagtaac ggaagaagtg
    10261 atggattcac agttggaagt ctcattgaaa gatgcacttt taatcaatcc agcatatgag
    10321 atcggtgata cgatccgttt tgaagtaaca ccaaaagatt ttgggcggat cgctgcgcaa
    10381 acggctaaac aagttatttt acagcgtgta cgtgaagcag aaagaacgat tatttataac
    10441 gaattcagtg cttatgaaaa agacatcatg caaggaatcg tcgaacgcca ggataaacgt
    10501 tatatttatg tcaatcttgg taaaattgag gcagttcttt ctaaacaaga tcaaatgcca
    10561 aatgaatttt atcaaccaca tgatcgaatc aaagtttatg tttcccgagt agaaaatacg
    10621 tctaaaggtc cgcaagtatt tgttagccgc agccatccag acctattgcg ccgcttattt
    10681 gagcaagaag taccagaagt ctatgatgga atcgttgaaa ttgtcagcat cgcccgtgaa
    10741 gctggcgatc gttcaaaagt ggcagttcgc tcaaatgatc caaatatcga tgcagtcggt
    10801 acatgtgtcg ggccaaaggg acaacgagtt caagcaatcg tcaatgaatt aaaaggcgaa
    10861 aacatggata tcgtcgaatg gaatgaagat ccagctgtat tcattgcaaa tgcattgaat
    10921 cctgcacaag tcatggatgt gatcttcgat gagacaaatc caaaggcctg cacagttgtc
    10981 gttccagact accaattatc gctagcaatc ggtaaacgcg gacaaaacgc acgtttagct
    11041 gctaaattga ctaaccataa aatcgatatc aaatcagaat ctgacatgac ggaattttat
    11101 gaaaatcaag aacaacaaaa agaaacagaa gaactgcatg atgaagcaat cgttcagtca
    11161 gatttaacag acgatgaata tgaaacaatt gcattcaaca atgaaacggt cgaagaaaaa
    11221 ccagaagctt aactttagta aggaggcaaa gagatgaaac aaaggaaaat tcctttacgc
    11281 aaatccgtgg tttcaggtga aatgaaacct aaaaaagaga tggtccgaat cacccgttca
    11341 aaagaagggg aagtatcgat cgatccaact ggaaaaatgc ctggacgagg agcgtatgtc
    11401 tcacttgagc cggaagaagt acaacaagct tgggataaaa aaattctcga tcgagtactc
    11461 gaaacaacat taacagatga gttttatcaa gaacttcttg actatgtcac tcaccaaaaa
    11521 gcacgtaaag agttatttgg aaaatgaata cagttaatcg gcaaaaagcc atgaatctca
    11581 tcggtctagc gatgcgcgcg ggaaaactaa tcacgggtga agaactgacc attgctgaaa
    11641 tccgcaaaca aaaagcaaaa ttggtgtttg tggcaaccga tgcaagcgag aatacaagaa
    11701 aaaagataaa agataagagt tcatattatg aagttccttg ctttgagcta ttttctgaag
    11761 cagaaatcac gcagatgatc ggaaaacctc gcaaggtatt cggaataatg gatgccggat
    11821 ttgcgaagaa gaccaaggag ctaattgaag gttaggaagg tgattgcatg ggcaacaaaa
    11881 gaatctatga attagcaaaa gaactcaaaa aatctagtaa agacgtcgtg gaaaaagcac
    11941 aacaacttgg catcgatgtg aaaaaccata tgggtgcgat tactagcaat gatgagaaga
    12001 aattgcaaca agcgttcaaa aacccaagca cgaaacaaca agctgcacaa caagcaagca
    12061 gaaaaccagc caatcaacag ccgaacgatc aacgtaaaaa agaaacaacg aacaatcaac
    12121 aaaaaaataa aaataaccgc agttatcaag atcgcggtca agggagcggt caagtgaatc
    12181 aaggaaaaaa accaacaaca aaccaaaata atacgggaaa taccagcagc caaaatcgtc
    12241 aaggaacgag ccaaggcggc aatcaaaacc gtactaacaa taacaacaaa ccaaacaata
    12301 acaatcgcgg caaatttaat aataataacc gtaatcgtta taataaaaaa ggtaaaaaag
    12361 gcaaacagca acaatcaact aaaccagctg taccaccacg caaattccgt gaattgccag
    12421 atgtattaga gtatactgaa ggtatgaacg tagcagatat tgcgaaaaaa attcatcgtg
    12481 agccagcaga aatcattaaa aaattattta tgcttggtgt catggtcaat caaaaccaag
    12541 cactggataa agatacgatt gaactgttag cgacggacta tggcatggaa ccacaagaaa
    12601 aagtccaagt tgacattgct gatatcgata aattcttcga ggcagaagaa gtcgatccag
    12661 ataaattagt ctcacgtccg ccagtcgtaa cgattatggg acacgtagac catgggaaaa
    12721 caactttatt ggatacatta cgccattcac gtgtaacaag cggcgaagcc ggcggaatca
    12781 cacagcatat cggtgcctac cagatcgata ttgatggtaa accaatcact ttcttggata
    12841 caccaggaca tgcggccttt acaagtatgc gtgcacgtgg agcaagtatc acagatatca
    12901 cgatcttagt tgttgctgca gatgatggcg tcatgccaca aacagttgaa gcaatcaacc
    12961 atgccaaagc tgcaggcgtt ccaatcatcg tcgctgtaaa taaaattgat aaaccaggtg
    13021 caaaccctca acatgttatg caagaattaa gtgagtatga gttaattcct gaagcatggg
    13081 gtggagaaac gatctttgtt gagatctcag cgaaattcgg ccaaaacatc gaagaattgc
    13141 tggaaatgat cctgttagtc gcagaagtag aagatttaaa agcagatcca acacaacgcg
    13201 caatcggaac agtgatcgaa gcacgtcttg ataaaggtaa aggtcctgtg tcaaccttac
    13261 ttgtccaaga aggaacactt catgttggtg acccaatcgt tgtcggtaat acttacggac
    13321 gtgtccgtgt aatgacaaac gatcttggcc gtcgcgataa agaagcagga cctgcaacgc
    13381 cagtagaaat cactggcttg aatgatgtac ctcaagcagg tgaccgtttc gtagtcttcg
    13441 aagacgaaaa gacagcacgt gcagcgggtg aagaacgcgc gaaacgtgca ttattagaaa
    13501 accgtgctgc cacaagccgt gtgactttgg ataacttatt tgaaagcctg aaagaaggcg
    13561 aattgaaaga agttaacgta atcatcaaag cagatgtaca aggttcagca gaagcactgg
    13621 ctgcatcatt gaagaagatt gatgtggaag gtgtccgtgt aaaaatcgtc cattcagctg
    13681 tcggagcaat caatgaaagt gatgtaacct tagcagcagc cagcaacgct attatcatcg
    13741 gtttcaacgt tcgtcctaca ccgcaggcaa aagtgcaagc tgatactgaa gaagtcgata
    13801 ttcgtcttca ccgtatcatc tataaagcca tcgaagaaat cgaaactgcg atgaaaggga
    13861 tgctagaccc tgaatttgaa gaaaaaatca ccggtcaaat gatcgttcgt gaaaccttca
    13921 aagtttctaa agttggtacg attgcaggtg cctatgtaac agaaggatat attcgtagag
    13981 atagcggtgt ccgtgtaatc cgtgacggaa tcgtaatcta tgaaggccaa cttgcaagtc
    14041 tgaaacgatt caaagatgac gtgaaagaag tcaaaatggg ttatgaatgt ggtgcaatga
    14101 ttgaaaaatt caatgacatc aaagtagatg acgtaattga aggatttatc atggaagaag
    14161 tcaaaaatga ctaaaaactg ataaaaggag gccaatcatt atggctaact accgtgaccg
    14221 ccgagtcggt caagaaatct taaaagaagt caatgatatt ttgcgcaaaa aggtccgtga
    14281 ccctcgtgtg gagaacgtaa cgatcacaga tgtgaaagtt acgggggact tacagcaagc
    14341 aacgatctat tacagcattt tatctgactt agcatcagat aagaaaacag cacaagatgg
    14401 attgaataaa gcatcaggat tgattcgtcg agagttaggg cacaatctaa gtatttataa
    14461 gacacctgaa ttgttctttg aacttgatga atcagttgtc tatggcaacc atatcgatga
    14521 attgcttcgc gacttgaaca aagactaatc aaaaactccc gcaaatcctg tgaaatcagg
    14581 ggttgcggga tttttttgtt ttcattaaga cgtattacct aagagttcca tgactttagt
    14641 cttgcggcgt gtcagctaac caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt
    14701 ttttctagta gtatataaat ttttgcatat agataaagat caagataccg ataacggaag
    14761 ctagattgta atctatttta gtgacagtca ggtatagcca agtaactaga agatcagtgg
    14821 aacctgcacc ataaaagttt gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt
    14881 tgaagttatt gatatttccg attacgccgc ttcgatttgg tcacgatcca aattttggat
    14941 aatccctgta gaaattagca tagtagctgg gataccaatt cacatattga caaagataac
    15001 agagactttt acataaaacg gatcaatcag gaaaggaatt gctcgatcca gccccagttt
    15061 tccagcaatg tgttaactgg cccagcgtca ttcagcaaat tatgcatgat gaggagcgaa
    15121 atgaactgcg gaacagccat tgtgataacg acaagttttt tggatcaatg taaaaaacgt
    15181 cagcgaaagg aaaaatttcg tggtttttta tacctgttca tttggctaga tataaaatcc
    15241 aaacaaatga acagaagtgc ttattaggtt tcaattggga gtatctgcgc tagaataaga
    15301 gtaataatga aaaggagtgt tatgtatgga ttattctagt aaagaatact ttgataaaat
    15361 gacggcttgg tggcgtgctg ctaattatct ttctgttggg caactttatc ttaaagataa
    15421 ccccttactg agacgtacac tgaaacccga agatgtcaaa aaacatccaa tcggtcattg
    15481 gggaacgatt cctggccaaa attttattta tgtccatttg aaccgagtca tcaataaata
    15541 tgatttgaac atgttttata ttgaagggcc aggacacggc ggacaagtca tggtatcgaa
    15601 tgcttattta gatggaagtt acacagagat ctatccagaa gtaacggaag atgagacagg
    15661 aatgcaaaaa ttattcaaac gattttcatt tcctggaggc attgcttctc atgcagcacc
    15721 agaaacacca ggttctatcc atgaaggcgg agagcttgga tactctcttt cacatgcagt
    15781 cggagctgta ttagacaatc ctgaagtaat ctctgctgtc gtgattggtg atggtgaagc
    15841 agaaaccggt cctttagctg gaagctggtt ctcaaatgtc tttatcaatc cagtgataga
    15901 tggagctgtt ttaccgatcc ttcatttaaa tggtgcaaaa atagccaacc caacgatctt
    15961 ggcacgtaaa tcagatggag aattagcaaa ttactttaac ggacttggct gggaaccttt
    16021 ctttattgaa ggaaatgatt cagaaaaatt aaatcctgtt atggctgaaa aaatggatca
    16081 agcaatcgag aaaattaaat ccatccaaaa agaagctcgc ctgaagacag caacagatgt
    16141 cgtgatgcca aaatggccag tattgatcgt acgcacacca aaaggatgga ccggtccaaa
    16201 agaatgggac ggggaaccaa ttgaaggaac cttccgagca caccaagtac cgattcctgt
    16261 agatcaagaa catatggatc atgcagatgc tttactccgt tggctgaaat cctatgaacc
    16321 agaaaaatta tttgatgcac aaggacgtat tttagaagaa atcagagaaa tcgcgcctac
    16381 tggtgaccaa agaatggcaa aaaatcctat caccaatggt ggaatcgatc caagaccgct
    16441 tattatgcca gattggaaaa aatatacttt gcaatttgaa aaaccaggct ccgttaaagc
    16501 agaagatatg accgaacttg gtaaattcgt tcgcgagatc attgagaaaa atccagaaaa
    16561 tttccgtata tttggaccag atgaaacaaa atcaaaccga ttgaatcaag ttttcaaaac
    16621 gacaaatcgc caatggatgg aaaaaatcga accagaaaat gatgaatggc tttcgccatc
    16681 tggtcgcgtc atcgacagtc aattatcgga acaccaagat gaagggttct tagaagggta
    16741 cgttctgact ggacggcatg gttttttgca agttacgaat cattcttgcg agtggttgac
    16801 tctatgttga ctcagcactt caaatggatg cggaaatcac atgacctttc ttggcgaaat
    16861 gattatcctt cgctaaatct cattgcaagc tcaacagtct tccagcaaga tcacaatgga
    16921 tatagccacc aagatccagg aatattgaca catttggcag aaaagaaggc ggagttcatc
    16981 cgtgaatatc tgcctgcgga tgcaaataca ttgctcgcag tcatggataa agcattccgc
    17041 tcgtcagaaa aaatcaattt gatcatctct tccaaacatc cgcgtgctca attttactca
    17101 gcagaagaag cagccgtctt agtgaatgaa ggcttgaaaa tcattgattg ggcttcgaca
    17161 gcaaaagagg aggaacctga attggtcatt gctgctgcag gaacggaatc taacttagaa
    17221 gcattagcag ctgtaacttt gctgctggaa gaattcccaa aactaaaaat tcggttcatc
    17281 aatgtcgtag atttattgaa attacgtcat ccaagtcaag atccgcgtgg gttgagcgat
    17341 gaagaatttg atcaatattt tacaaaagac aaaccgatct tatttgcttt ccacggttat
    17401 gaaacattgg tccgcacgat tttctttgat cgtcataacc atcatttaat gattcatggc
    17461 tacaaagaaa atggagatat cacgacacca tttgacatgc gtgtagtaaa tgaacttgat
    17521 cgttatcatt tggcaaaaga cgcagcttta aaaatcaaag gaagtcaagc agaagatttt
    17581 gcgaaaaaaa tggatcagaa attacaagaa catcaaaact atattcgtga aaatggtatt
    17641 gatctgccag aagtattgga ttggaaatgg aaaaatcttg accaataaaa tgataaaatc
    17701 gatcccgcaa aatagaattt tttacattga ttaagaaact gaaaaaataa ataaaaaaaa
    17761 cggaaaaaag actggaagaa tggccagtct tttttttcgt ttttgggaag tgaaaaccaa
    17821 actatttacc tgtaagccac ccataaatcc aatcaaatgg tgcaaagaaa aaatcttcca
    17881 ttctattccc tcctttaaaa aagcataaca tgatttctat ttacgtgagc atgaattttg
    17941 ttacgttttt attgccgaaa aataaaaacc gttcattatg atgtcaaatt cgtcagtaat
    18001 tgatgcaatg tgttataata attcagttaa atcaaaaatg aaatggagaa aagacatgga
    18061 cgggttatta cctttatgga aagaacgcgg aatgacaagt catgattgcg tattcaaatt
    18121 aagaaaaatc ttacatacga aaaaaatcgg acatgggggt acgcttgacc cagacgtaga
    18181 cggtgtgttg cccatttgta ttggaaaagc cacaaaagtt atcgaatatt tgacagattc
    18241 tgggaaaatc tatgtaggcg agataactct aggtttcagt acaacgacgg aagatcgatc
    18301 cggagaacgg gtagaaacaa aaccagtaga tatgtcttta tctgaaaaaa aaatagatga
    18361 agtgatggct tcatttattg gtgagatcac ccagatacca ccaatgtact cagcggtaaa
    18421 agtcaacggt aagcggttat atgaatatgc ccgcaataat gaaacagtgg agcgtcctgt
    18481 tagaaaggct cagatttacc gttttatccg gacaagtgaa ttgacaagag acgaagtgac
    18541 aggaaccatt tcttggcgct tcgaagtaga atgcgggaaa ggaacctatg tccgcacatt
    18601 agctgtagat acaggcaaga aacttggtta ccctgcgcac atgtctgatc tgacccgaac
    18661 agcaagtgcc ggtatgtccc agaaggatgc tctgacatta gaagaagtag ctcaagctgt
    18721 gaaaaatggg acaatcgatg attgtttgct gccaatagaa aaaggagtgg aacggttcaa
    18781 acgagtagat attactgatg aagtatggca gaaagtaaaa aacggtatga gacttgatta
    18841 tactgtattt gcgctcccag aaatgccgtc agaagaaatt gctttgtttt ataaaggaaa
    18901 agttgtcagt atttatcagc caaacccgaa agaaaaaaac aaattaaaac ctagtaaagt
    18961 attgagaaat gaggaataac gaatgaatat catcaagatc agacatcctt accaatcaga
    19021 acaaattcca gaagacgatg tcgttttagt gctgggattt ttcgacggcg tgcatatcgg
    19081 acaccagaaa gtcataaaga caggtatgga aattgcacaa aaagaaggat tgaaattagc
    19141 attgatgact ttcaatcagc atccatctat tgtctttaaa aagatcgatc ctagcagcgt
    19201 gaaatatttg actagcctta agcaaaaaga agaaaaaatg gccgctttgg gtatagatta
    19261 tttatatgaa gttgatttta cttcttcttt tgctcattta gcaccgcagg aatttgttga
    19321 tcaatacatc gtagggttac atgcaaaata tgctgtttcc ggttttgatt atacgtatgg
    19381 accaaaagac attgcagatg tcgctcattt tcctgaatat gccaaaggaa gattcaaaat
    19441 cgtcacagtt tctaaagaag aagaacaagg tgaaaaaatc agctctaccc gtattcgtgg
    19501 attactagac agcggacaag taaaagaagc agcaaaacta ttaggttgca catatgaggt
    19561 agaaggggta gttgtccatg gcgatgcacg aggcagacta ctaggattcc ctacagcaaa
    19621 tgtcaaagta aagagcacgg ttcatttgcc taaagaaggt gtttatgtgt cggaaataaa
    19681 aattggggaa acatggtatc cagccatggg ttcaattgga cataatgata cattcggtga
    19741 aggacgggag ctgactgtag agttgtatat tttggacttt aatcaagata tctatggtga
    19801 acatgtcgcg gtacgctgga atcaattttt aagagatcaa gtgaaatttt ctagtgtaga
    19861 ggaattgatt gaacaactca aggaggatga acaagcaaca gctgaatatt tttctcagca
    19921 ataaaacatt atgcataaaa tctcttgttt atgtataact agcaattaca ataaaatggg
    19981 tgataaataa gcccaggaaa ggggctgtga caaaagtctt gtcacagcct ttttttccaa
    20041 ataaatggtt tttcagaagt agcttcttcg gaaataagcc gaaatttctt gaagctgacc
    20101 acttctgtca caacctcttt ctttatagaa ataaaaggac actagcaatt catgaaaacg
    20161 attgctagag cgtatcattt gctttagact atatatcttg gtgtacactc actttaaggc
    20221 ggaaataaaa gttatccgta ataacaggtt taggtatgaa aaaggagtgg gtactatgta
    20281 caaggaatta gaaaacaaag tcgcagtagt aactggtggc tcaaagggga ttggaacagc
    20341 gattgctcaa cgattcggga aagaaaaaat gaatgtagtt gtcaactata actcggataa
    20401 agaaggagca gaaaaagcag tcaaagcaat cgaagaggca ggcggaaaag cagtctctgt
    20461 acaagcagat gtcagttctg aagaaggaat acagcgttta cttgatacgg ctttagaaaa
    20521 ttttgggaca ctggacgttt gggttaataa tgctgggatg gaaaatcagc atccaaccca
    20581 tgaacttagc cttgaagatt gggaaagagt gttaaaagtc aatttgactg gcgtttttat
    20641 cggaacgaaa gcggctttaa attactttac agagaataat aaaaaaggaa atatcatcaa
    20701 tatgtcttct gttcatgaac agatcccatg gccaacgttt gctcattacg ctgcttcaaa
    20761 aggcggggta aaattattta ccgaaacggt agcaatggag tatgcaccga agggaatcag
    20821 aatcaatagt ataggccctg gtgctatccg tacacctatt aatgcggcaa aatttgatga
    20881 tcctgaacaa aaagaactct tggaaagtat gatcccacta ggacgcgtag gtgaaccaaa
    20941 agaaatagcg gcgacagctg catggttggc ttcagacgaa tccagttatg ttacaggtat
    21001 tacactattt gcagatggtg ggatgacttt atatccttct ttccaaggtg gaaaaggata
    21061 agggtgaata aaaatgggtt tattgattgc attgatcccg gcagttggct gggggatcca
    21121 gccactgatt ttgaaaaaga ttggtggcag accaacaaat gagatattag gaactggtat
    21181 tggagcggtg cttgtcggct tactcgttca attattcgct tctccagctg gtatttcagt
    21241 gactacattt ttgattagtt tactttctgg tgcattttgg gttattggac aaacaggaca
    21301 gtatacatct ttcaacttga ttggtgtttc taaaacgatg ccaatttcga ctgcattaca
    21361 gttagtcgga acctcgttga ttggtgtctt cgcttttgga gaatggtctg gaagtatggg
    21421 gaaaatcatt ggagcagcag caatcattct tcttgtattt ggttctgctt taacggcaat
    21481 cagtgatgat cctggaaaaa aaggcggtat cacaaaagga attagtctat tagcatcaac
    21541 aagtattggt tattgggtat acagtgcatt gcctaaacta gttgatgcaa gcggactttc
    21601 cattttcttt ccgcaaatga tcggtgtctt tttaggggca gtgatttatg tgattttccg
    21661 taaaccaacc gcattttctg aagacaagag ttggaaagca acgatcgttg gagccatctt
    21721 tagtgtgtca gcattggctt atattttttc agcaaatgaa aatggggttg ctactgcata
    21781 tatcattaca cagctaaacg tagttatctc tacattaggc ggtatgtaca tcctgcatga
    21841 aaataagaca ccaaaagaaa aaaaattaac gattatcgga ttattgctga tcgtagtagg
    21901 aagcatggtc acggtcttta tttgaggata aagatggtaa aatttcattt attaaaaaaa
    21961 tatttgtttg aaccaaacag taagtactta taaaaaaata gcgctagaat ttccaacatc
    22021 tctggaattt ctagcgcttt ttaattgttt aagatacaag ggaaatatct gtctggatca
    22081 ataatttttc attgatccat tgactaaagt ttacgacata attgaagtta ccaccgatta
    22141 gtatctccgc atgattgtct ttataaagat cgtattgtaa aagacggtct tttttatttg
    22201 cgtaaacatc gttatggtac tggactaatt gttctcggaa ttgattgatt gctgtaaaat
    22261 cacgatattg aaggttgaaa cgcatcgtga ttggtgcatt tagcatgaat cgctgcattt
    22321 cttttgctgc ttcctctgct tgatctccgt ataggaactc aaaaagatta cgttcagaaa
    22381 atttccattg cccgccaacc tttgttcctt ttagttttcc ttcatgaaga taatttctga
    22441 tagaacgtgt agtaacacct aattgttcgg ctaattcatt tactgagtaa aaatctttca
    22501 atgttcaccc tcctatgtga atggttctac tgacttagtt gatatttgct taatcacgaa
    22561 atgtagaaag aacgatttta attaaggtca ttgtaaaaat caagtcagaa aattctaact
    22621 tttatttctc taaagaaat
//
LOCUS       DAPCMU010000033        22517 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPCMU010000033 DAPCMU010000000
VERSION     DAPCMU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.033"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(26..223)
                     /locus_tag="RD744_001035"
     CDS             complement(26..223)
                     /locus_tag="RD744_001035"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDU0557053.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(369..1247)
                     /locus_tag="RD744_001036"
     CDS             complement(369..1247)
                     /locus_tag="RD744_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0557054.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="RD744_001037"
     CDS             complement(1286..2404)
                     /locus_tag="RD744_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDU0557055.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="RD744_001038"
     CDS             complement(2401..4500)
                     /locus_tag="RD744_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDU0557056.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="RD744_001039"
     CDS             complement(4551..5372)
                     /locus_tag="RD744_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0557057.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="RD744_001040"
     CDS             complement(5369..6178)
                     /locus_tag="RD744_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557058.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="RD744_001041"
     CDS             complement(6199..6693)
                     /locus_tag="RD744_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0557059.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="RD744_001042"
     CDS             complement(6705..7193)
                     /locus_tag="RD744_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0557060.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7270..9966)
                     /locus_tag="RD744_001043"
     CDS             complement(7270..9966)
                     /locus_tag="RD744_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0557061.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10394..11467)
                     /locus_tag="RD744_001044"
     CDS             complement(10394..11467)
                     /locus_tag="RD744_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0557062.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11464..12291)
                     /locus_tag="RD744_001045"
     CDS             complement(11464..12291)
                     /locus_tag="RD744_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557063.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12292..13098)
                     /locus_tag="RD744_001046"
     CDS             complement(12292..13098)
                     /locus_tag="RD744_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557064.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13110..14195)
                     /locus_tag="RD744_001047"
     CDS             complement(13110..14195)
                     /locus_tag="RD744_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557065.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14212..14754)
                     /locus_tag="RD744_001048"
     CDS             complement(14212..14754)
                     /locus_tag="RD744_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0557066.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15012..16001
                     /locus_tag="RD744_001049"
     CDS             15012..16001
                     /locus_tag="RD744_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDU0557067.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15998..16735
                     /locus_tag="RD744_001050"
     CDS             15998..16735
                     /locus_tag="RD744_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDU0557068.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16732..17283
                     /gene="coaC"
                     /locus_tag="RD744_001051"
     CDS             16732..17283
                     /gene="coaC"
                     /locus_tag="RD744_001051"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDU0557069.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17299..17931
                     /locus_tag="RD744_001052"
     CDS             17299..17931
                     /locus_tag="RD744_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0557070.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18016..18663
                     /locus_tag="RD744_001053"
     CDS             18016..18663
                     /locus_tag="RD744_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0557071.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18668..19651
                     /locus_tag="RD744_001054"
     CDS             18668..19651
                     /locus_tag="RD744_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDU0557072.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20054..21568)
                     /locus_tag="RD744_001055"
     CDS             complement(20054..21568)
                     /locus_tag="RD744_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDU0557073.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21706..22512
                     /locus_tag="RD744_001056"
     CDS             21706..22512
                     /locus_tag="RD744_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557074.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6800 a         4832 c         3903 g         6982 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaattcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatctgttta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttttc atagctacaa
    10081 atattgaact ggattcatat attagtttta ttaatagatt acagaagggt taagtatatt
    10141 tgcatagttt ccaattactt tatccttctg taactttttc aatcttagat ttactagccc
    10201 gtctcgggcc ctattttaca atggttttgt ccacttttct ctggtagaaa aacgtggtag
    10261 aaaaaataaa ataacacaaa aaaagcggcg tttcatccgc ttttggtaga agattggaaa
    10321 actcagatag aaaaaggacg atttttagct ctttagaacg ttgataagtc cccattctta
    10381 ctgagttttg ccattatctc ctatacatct tgaattctaa gaataggtca ttatagatgc
    10441 ctaggtattt cgatcctaga tctttgtaga cttcaagatg ggagatcgtg tcatcagatg
    10501 gatagaattg ttcgtccgac gaaatgtctt ttggcaataa cgcttttgct tcttcatttg
    10561 gtgtagaata accaatatac tctgcatttt gtgccgcatt ttccggttct agcataaagt
    10621 tgatgaaatc ataggcacct tctttatttt tcacggtttt tggcataacg atattatcaa
    10681 accaaagatt ggatccttct ggtgggatca cataatgtaa atgttcattt tcccacatca
    10741 tatcggcagc ttcgccagaa aatgtcactg ccacggaggc ttcttcattg atcatataca
    10801 ttttgatctc atctgccacg attgctttga catttggtgt taaatgattc aatttatttg
    10861 ctgcttctgt caactgtgcc atattcttac tattcaaaga atagcctaaa ctattcaaag
    10921 aaagccccat aacttctcgt gcaccgtcaa tcagcatcaa actgtctttt aattccggct
    10981 tccacaaatc atcccaatgc ttaatttgat ttgcatcaaa gaatttatcg ttatatacga
    11041 tccctaaagt tccccaaaaa tatggaatcg aatactgatt atctggatca aaatccaagt
    11101 ctaaaaatcg tggatcaata ttatttaatc ctttgatctt cgaatgatct aatggaagta
    11161 ctagtttttc tttgatcatt ttttggatca tatattcact tggtatagcg atgtcgtagt
    11221 ttgtcccacc ttgctggatt tttgtgaaca tcgcttcatt agaatcaaat gtttcataat
    11281 tcactttgta tccatattct ttttcaaatt tcgtgatcaa actaggatcg atataatctc
    11341 cccagttata gatggtaagt acctttgctc cactcatgcc acttgttttt tcaagttgac
    11401 gaacaccaag cagcagcacc gcaataatga tgagaatacc taaaaacaga gattgtaatt
    11461 ttttcatcgc agccgtgcca ccccctcgcg tactcttctg tttttcttca gccgttttgg
    11521 acggttttct ttactaataa agtagtatcc aatgaccaat atcatggaga acaagaaaac
    11581 taacgcactt agcgcattga tttctaaact gattccttgt cttgcacgag aatagatctc
    11641 tactgagagc gtcgtaaagc catttcctgt gacgaaaaat gtaaccgcaa aatcatcaag
    11701 agaataggta aatgccataa aatatccagc tatgattcct ggagaaagat aaggcaaaat
    11761 gatatttttg actacttgga aattattagc accaaggtct ctagccgcat cgaccattga
    11821 atcgttcatt tcctgcaatt ttggcaagac catcaagacg acgattggaa tgctgaaagc
    11881 aatatgggaa agcaatacac tctgaaaacc taatccgaat cctagtagtg taaagaaaat
    11941 caagaaacta gcaccgataa taacatcagg agagactaat aagatgttat tcatgctcaa
    12001 caacgctgtc cgtgcacgtc gttttttcgt gtaatagatt cccatagccc caaatgtccc
    12061 gatgatcgtg gcgatcaatg cagataagaa agccagcatg aacgtattca atacgataat
    12121 gatcaaacgt gtatcttcaa aaacagcttc ataattttcc caagtaaatc ctgtaaagct
    12181 gttcatcgtt cctccttcat tgaaggaata aaaaatcaga tagaagatcg gtgcatataa
    12241 gaataaaaag acgatcccta aataaagata cgaccattta aattttttca tttatgagcg
    12301 acctcctttt ttcttctcgc ctgtcaggag catcacgatg aacatagcaa tgattaaaat
    12361 cacaccgatt gtagaaccca tcccccagtt ttgtgtaacc aagaaatgct gttcgatagc
    12421 cgttcctaaa gtgatcaccc gatttccgcc aatcagacga gtcagcatga ataaagaaag
    12481 agaaggaata aatactgctt ggacaccact tttcacacca tttaatgata acggaaaaat
    12541 cactcgtcgg aaggtctcaa agctattcgc gcctaaatct ctactagcac tgatcaaaga
    12601 aggattcatc tcttccaatg cattgaaaat tggcataatc ataaatggaa tctcgatata
    12661 cgttgccacg aacagaaaac tgaagtctgt gaataatatc tgtttcggcc cgatcccgat
    12721 aaaatcaaag aaatgattca cgctgccgtg cacactgaaa atcccaataa atgcgtaagc
    12781 tttaagcagc agattcaccc atgtaggcaa aataatcagc atcagccata attgcttgtg
    12841 cttcagtttt gttaaaaaat aagcagtcgg gtaactgatg atcagcgtga aaaacgtgat
    12901 caaaaatgca taccatacag aattgatcgt catagaaagc gatgtccccg acgtaaaata
    12961 agatagataa ttattcagcg tgaattgccc attcatatca aaaaaagatt gataaatgat
    13021 cattaataca ggcgcgatca cgaataaacc gagccacaaa atataaggga cggaatagat
    13081 cctgcgcatt gttttcattc ttgtcccccc tattcttcgt agctgtcaag acgtgcatcg
    13141 aattcttctt cagactcgtt gaaacgcatg acatggatat cttctggctc aaaggccaat
    13201 cctactttgc ttccttcttt agcttttttc gttgaatgga ccatccattc attttgctgt
    13261 tcatcataac agatgatttc ataatggaca ccacgaaaca gctgagtatc gaccgttacg
    13321 actagttttc ctttatctgg tgtcgtgatc gtcaaatcct ctgggcgtaa aacaacttca
    13381 actggttcat ttagacgcat cccgccatcg acacattcaa attgcttacc aacaaactca
    13441 actagattat cttcgatcat tacaccgtcc acgatattgc tctccccaac aaaatcagca
    13501 acgaaatgat tgattggctc atcataaata tctacgggtg taccactttg gacgattttc
    13561 cctttattca tgacaaaaat ctcatcactc attgccaacg cttcttcttg gtcatgagta
    13621 acaaaaataa acgtgattcc taatcgttgt tgcagatccc gcagttcata ctgcatatct
    13681 gtacgtagtt tcaaatcaag cgccgataac ggttcatcta atagcaatac tttcggttca
    13741 tttacaattg ctcgtgcaat cgctacacgc tgtctttgac caccagacat ttcactaatc
    13801 tcccgttttt cgtatccagg aagctggacc atacgcaaag catcttttac cttcttttcg
    13861 atatctgatt tggacatttt cttgatcttc aatccaaaag caacgttatc aaaaacattc
    13921 atatgaggaa aaagtgcata atcttggaag actgtattta cttgtcgctt attagctggc
    13981 aaatcattca accgtttccc ttcaaaatag atgtcccctt ctgtagcttc ggtgaatcca
    14041 gctataatac gtaggatcgt tgttttacca cagccggaag gaccaagcag tgtataaaat
    14101 tttccttgtt cgatctcaaa acttactttt tttaatactg tttcatcatc atagcgtttc
    14161 actacttgat caaatgaaat gattggtttc cccacgtttt taccccctat attacaaata
    14221 tgattgcgtt gcaacaatca atacttttgt tcgttttttg agatgattcc ttaattgatg
    14281 ttgttttgtc gcttgataat agatcgactg tcctgctttt gccacataga ccgtctctcc
    14341 taagcttaac tctaattccc cttctaatac aaaaataaac gtttcagata atgaaggttc
    14401 aaacgtctta tattctcctg caccttcaaa agtcagcaag acaggctcca tttctttttc
    14461 attcgaatca gccacgagcc attccaattc atacccgttt tcttcatcga aataaaccgt
    14521 atgatcttct tttgaataaa ccacttgcag attcgctgct tcttgatgaa aaaattcttc
    14581 tggagtcaca cctaagactt caagaataga aaagaatgtt tccattgatg gtgaacttaa
    14641 atctcgttcc aactgggaaa tatacccttt tgacaaatct gtgcgttcgc ctagttcttc
    14701 ttgtgtcaaa ttcttttgga tgcgcaaatt tcttaacttc tcaccaattt ccataatttc
    14761 ctccgaatcg aagttttcta atagtaaact tcctgtttac taccactcgt attagtatac
    14821 gaaatttatt gaaaaaggca accctttgta aaattttttt tgctgaatat ttctttatct
    14881 tttggaataa attctagttg tttgattctt tatttttcac ctaatttttg tctaaattta
    14941 cggaagaaca agaataaaag agtaggcttt atggtatgat aaaagaaaat tttaaaagga
    15001 gtgtctcacg aatgaacgct tttcgatctt ttcaggtaat aaaggaacct gttttccata
    15061 cagcaatcag tgatctgcca tttactgagt tggctgaaaa tgaagtcttg gtaaaaattg
    15121 cctattctga tgtcaattat aaagatgcat tggcaatgag cgagtctggt caggtgatac
    15181 gaacatatcc gatgactcca gggattgatt tatccggaac tgtcgtccaa agtaacaatc
    15241 cacgtttttc aaaagaagat cttgtacttg ctactggttt tgggcttggc gtcacccatc
    15301 caggcggtta tagccaatat caaaaagtac caggagactg gttagttcct ctaccaaaaa
    15361 atatgacgct gcgtcaagcg atgatccttg gtacagcagg gtttactgct atgttgtgtg
    15421 tgaacgcttt aatccgtcaa ggtatgacct ccgataaaaa agttgtcgtt actggtgctt
    15481 ctggcggtgt aggcagtaca gcgatagccc tgttacataa actaggattc cagtcaatca
    15541 ttgccttttc cagaaaagaa gagtctgtca catggctaaa aagtctaggt gctttgcaag
    15601 tcgtacgacc tgatgaattt ctgccagaga caacaaaagc tttaggaaaa cagcaaatcg
    15661 actatgtcat cgacacagta ggtggcgagc agctaagcag tcttcttcca ttgatctctt
    15721 ataacggtgc agtagcttta tgtggcaatg ctgggggaat caaactaaac gcgactgtct
    15781 tgccttttat tctgcgaaac atccagttga ttggaattga ttctgtcaat gtaccaatag
    15841 atcaacgtct ttctttatgg caacagatgg cggatttgca gattgcagat gaactggtcg
    15901 tccaagagat tactctggat caattgcctg aaacagcatc taaactactt gctggaacac
    15961 accaaggaag aacactagta aatgtaggtg accataaatg aagattctaa tcacagctgg
    16021 aggaacgact gaaaaaatcg atcaagtacg tgctattaca aatcattcga caggacgatt
    16081 agggcaagca ttagctgatt atttagctgc caatccggat accacaatcg attacgtgac
    16141 tacgaaacaa gcactgaaac cgaaaagaca ctccaatata acgatttata cgatcgaatc
    16201 tgcactggac ttatttttac aattagaaga actaacgaaa aaagaacact acgatgcgat
    16261 tatccacagt atggcagtca gtgattttac tcctgctttt agtttttcag aagaacagct
    16321 ggcaaaaaac ctacccactt cttcaacgca agaagaactg gacaattggt ttgcggaaaa
    16381 tgaacagaca gataccactg tttcaaagat ctcttctaac accgagcatc ttgtgctcgt
    16441 tttgaagaaa acaccaaaaa tcatttctta tctacgtgaa tggcagccaa aagcaaaaat
    16501 cattggtttc aaactattag tagatgtacc aaaggaatct cttctggcag tcgcaaaaaa
    16561 tagtctgata aataacaaaa ctgattttat tttcgcaaat gatttgacgg aaatccatgg
    16621 cgagacacac cacggctatc tcttatctaa agatggaaca gtggaagaag ctcaaagtaa
    16681 atctgagatt gctgcattga ttactgagaa aattagactg gaggaatcaa aatgaaaaag
    16741 atcttactag gcgttactgg aagtatttct gcttacaaaa gcgctgatct tacaagtcaa
    16801 ttcgtcaaat taggtgccca ggtagatgtg attatgtcta aaagcagcac acattttatc
    16861 acgccattaa ccttgcaatc tctgtcgaag cgcgcagtcc atacagacgt catgcaagaa
    16921 tcaaatccgc aggtaatcaa tcatatcgaa ctagcaaaac aagctgactt gtttctcatc
    16981 gcgcctgcta cagcaaacat tatcggcaag ctagcaaacg gcattgcaga cgatcttctt
    17041 tcaacagtcg caatggcgct tccagtggga acaccaaaat tgattgcccc tgccatgaac
    17101 acgaatatgt atcaaaaccc gatcactcag cgtaatctgg aaacattgag atctgtcggc
    17161 tatcaggaaa ttgaaccaag agaatctctt ttagcttgtg gagattttgg aaaaggtgcc
    17221 ttagctgata ttcagacgat catcgaacag gtcatgctga ctcttaatga aagaaatgct
    17281 tagaaaaaag gagtacttat gaaaaataca aaaaacttta cattaactgc gatgttttta
    17341 gctattttga ttttattagc tgttacgcct ttaggtttta tccctatcgg tccaatcaat
    17401 gcaacaacca tgcatattcc tgtaatcatt gcttcgatcg ttttaggacc aagactaggt
    17461 gcttttcttg gcggtacatt tggactgatc agcatgatcc gtagcacagt tatccaaaca
    17521 cctttgtctt ttgtcttttc tccgtttatc ccagtcatcg gaacagaaca tggaagctgg
    17581 aaagccttgc tgatcgcgtt tattcctcgc atcttgattg gggtggttcc ttatttcgtt
    17641 tataaaggtc tgcgcaaact aatgaaacaa aaagcagatt ccgtttcttt attcttagct
    17701 ggactttccg gttcattgac caatacgatt ttagtgatga atatgatcta ctttttattc
    17761 cagcaagatt atgccaaagt catcggaaca aatttaaatg ctgtttattc agctattcta
    17821 gcggttattt ttacaagtgg tatacctgaa ggaatcgtcg caggtcttgc gactgcagca
    17881 gtatgcaatg tcttgttaag atacttcaag cacccttctc ttactaactg atagtcatcg
    17941 tagccgatct ctgatttttt tctcttttgg tgttactatt gaatggaaag atttgacgtc
    18001 gaaaggatga tccaaatgaa aatttttgtt gtaggtgcaa acggacaaat tggccgacac
    18061 ctgataaaag atttagctcc aagttcccat gaaatttttg caggggtgag agatgttgct
    18121 tcccaaacat tagtaaaaaa agagaatgtt tcgtatgtct cttttgattt aacttggtcc
    18181 gtggaaaaaa tgaccgaagc attcaaaggc atcgatgtat tgatttttgc tgccggctct
    18241 caaggaaaga atcttctgca agtagacttg gatggcgcaa tcaaaacagt gatcgcagct
    18301 gaaaatgcgc atgtctctcg ctatctgatg gtcagtgctg tttatgcaga tgaaccagca
    18361 aaatggcctg agtccatgac agattattat attactaaac attatgcaga tgaatggctg
    18421 aaacgaacta acttagattt tgtcattctt caaccagtca cattgactaa cgatgaagaa
    18481 gttacttcca ttcaattaac taagcctaat gagaaggcat caaaaacgat cactcgctct
    18541 acagttgctg ctgtacttgc cgctctagtg gaagaaacgg atatttctcg tacgactcta
    18601 gtactttctg aaggtagcaa agagttgaat acagcattcc aagaatgggc aaaggaggaa
    18661 tagatccatg cgtgccattt caatcaagca tcctggttct gtagatgtat tagaacaaat
    18721 tgaagtgcct gttcctaaac caaaacaagg cgaattactg atcaaagtcc atacagcagc
    18781 tgtcaatcga acagatatta tgaaaagaga atcagtctct ttgaaacctc cctaccctat
    18841 tttgggagtc gaagtagctg gagaggtagt tgagaataaa agtgatatct ccacttttac
    18901 tcctgggaca aaagtatacg gattagtcaa tctaggtggt tatgccgaat atgctgtgat
    18961 gcctgccgat cgtgcaatcc ttctccctga tacgctagac tatgtttctg ctgctggaat
    19021 ctcagaagtt tttttgactg cttatcaaac actttactgg cttggaaaat tacaagaagg
    19081 cgaaactgtc ttgatccatg caggagcaag tggtgtggga acagcagcga tccaattagc
    19141 tagacagctt tctcacgcga aaatcatcgt tactgcagga tcagaagaaa aactcgcctt
    19201 ttgtaaagaa cttggtgcag atgaattgat caactataaa aaacaagact ttcaagaaga
    19261 ggtacgaaaa atcactgaag gtcggggagt cgatgttatt ttggatttca tcggcgcttc
    19321 ttactgggaa aagaatttag cttctatagc cgttgatgga cgttgggtct tgattggtat
    19381 gctaggtggg accatcgtgc ctgagataga ccttggtgta ttgattggga aacgtgtcca
    19441 actgcttggt acattattga cacctagaag tgatcagtat aaagcagagc tcgcacaaac
    19501 ttttatgcaa aaagctgggc cttttctaga aaatggcaag attcggccaa tcattgaccg
    19561 tacattccaa ttcagcgcag tcaaacaagc gcatgaatat atggaagcaa acaaaaacat
    19621 cggaaaaatc attttgaaga taactgaata aaatcatgct gatcaaaaaa gaggctggga
    19681 cagaagcgtt taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt
    19741 gtgaatttca gcttatttcc gaaggaattt cttctgcttc cgccgtttat acgtgtttag
    19801 agcgtgagac aaaagtgttc tttacttttg tcccacgctc tttttccgaa taaacggtgt
    19861 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag aagctgtttt
    19921 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct tcactaaaca
    19981 gtgttcaatc agctgaccct cggtattagc tcaaaacgct gatttcacaa cctcttttta
    20041 tttacgttct atttcattca ttttccgaag caaatcgagc tgcttgtatg cccgcttgtc
    20101 gcccaaaaat aatgatctcc gcaactgaat ttccaccgat ccggttgttt ccatgtaagc
    20161 cacctgtgac ttcaccagca gcatataacc ccctaattgg ctggttttct ttatctaata
    20221 cttcagtgtt cgtattgatt ttcacaccgc ccatcgtgta atggattcct ggtgcgattt
    20281 tgatcgcata ataattcgga gacgataagt catgttccat ggctgtggca cggttgaatt
    20341 gagcatcttt tttctccgca acatccgtat tccatccatc aactgttttt gctagtgttt
    20401 cttttggtaa atcgattttt tctgctaatt cttccattgt ctttccagtt gtgacatatc
    20461 ctttttggtc ataaaattca atagctgtgg cacgatcacg cacgccttgg tcaaaaatca
    20521 aataagctga tttttcaggc aatgctgtaa ttgctgcaga tactttatcc cttgtatcca
    20581 tttcattgac aaatcttttc ccctcttgat cgaccaagat tgctccttct ccgcgaacaa
    20641 cctcaccaat caagaccccc tcatcctgct gcacagttgg atgaatttgt attttatcca
    20701 tatcaacggt ttgtccacct aatttttcaa tcatggtgat accatctcca gttgatcctt
    20761 cttgatttgt cgtaatataa tcagctaaat cgggacgata tttttcaata aattctttgc
    20821 tggcaccaaa accgcctgtg gtaactacga ttgcttttcc ggtgacctct tttggttctt
    20881 ctccttgaac atgtacagtc acttcattga ccgtttcccc atttttctta atgtctgtca
    20941 catctgcgtt gacaaaaata gggatttccc gctcatggac gttattcaat aacccctcca
    21001 ctaagtatcc accaatagcc gagccatcag acggacgatg tgtcctcttt tcactcatcc
    21061 ctccagtgat cgttaagttg tccagtttga taccgttttg atctagccaa tcgaccgcat
    21121 cagcagagtg atcgacaaag tatcgaagca tttctttatc attcgttcct ccaccacctt
    21181 ttagcgtttc ttcatagaag gcatcattcg agtctttgat tccttcttct ttttgaaatt
    21241 ttgtttctga agcattcata ccgcttgatg atttagatgt gtttcctccg gcaacaggca
    21301 tcttttccaa aattactgga ttcaacccag catcttttgc ttcgatagct gctgccatcc
    21361 ctgcaccacc agctccaata atcacgacat cataagattc tttcatttct tttggatctg
    21421 tatatacttg ttcagaagcg cctgatgata cttcggcaga ttccttcgta ctagaacttg
    21481 tctgcgctgt atttttctga tctgcgccgc atgcgctcat agcaagaagt gcaacggctg
    21541 ttaataaacc agcaactaat tttttcattt gtatccccct tttctcctta tactatatta
    21601 agtgattttt ttcacaatat caactctgtt tttctttaag tcatatagct ttggtaaaca
    21661 cacgcttctt agtataatta aggatataca tgaaggagtt attttatgaa tcatttgaaa
    21721 acctttttta cgaacagtac aaaaactgca ggtagaatca caaacggaat ctatatttta
    21781 ttacgtatca tcgtcgttat tatgctagtg gatcaggtct ggaaccaaca ctggggaaat
    21841 acaatgcttt tattactgac attaatttta tttgaaatcc cttattttct ggaaaagata
    21901 ttaaagatac agattccaaa tatgctagaa atcattttgt tatcttttat ttttagttca
    21961 acagttttag gagagttaag tgatttttat ggctattttt caatgtggga tacttttcta
    22021 catgcattca gcggattttt agctgggggt gtcggctttt cccttgttta tttgctcaat
    22081 aaaaatacaa aaaccatgaa cttgactcct ttattactag cagttgtttc cttctgtttt
    22141 tcgatgacag ttggtgttat gtgggagttt gtggaatttt cagcagataa attactgcaa
    22201 ttcgatatgc aaaaagatac gatcatccag caaatcaata gtgtggagtt aagtaaagac
    22261 ggaaacactg tctatcatgt tccagatatc actcggacaa tcattgaaag tcaaaatgca
    22321 gatggtaaaa cagataaaac aatcatccaa aatggttact tagacgtggg aatcatggat
    22381 actatgaagg atttatttgt taattttatt ggagcagttg ttttcagtgt atttggttac
    22441 ctctatagtc gaaacgatta ccaaaaattt acgttcatca agaattttat tcctaaaaaa
    22501 atcaaacgat aaaacat
//
LOCUS       DAPCMU010000034        22087 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPCMU010000034 DAPCMU010000000
VERSION     DAPCMU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.034"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            106..1038
                     /locus_tag="RD744_001057"
     CDS             106..1038
                     /locus_tag="RD744_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557075.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1259..3022
                     /locus_tag="RD744_001058"
     CDS             1259..3022
                     /locus_tag="RD744_001058"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDU0557076.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3106..3564
                     /locus_tag="RD744_001059"
     CDS             3106..3564
                     /locus_tag="RD744_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDU0557077.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3673..4356
                     /locus_tag="RD744_001060"
     CDS             3673..4356
                     /locus_tag="RD744_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDU0557078.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4503..5117
                     /locus_tag="RD744_001061"
     CDS             4503..5117
                     /locus_tag="RD744_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDU0557079.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5133..5339
                     /locus_tag="RD744_001062"
     CDS             5133..5339
                     /locus_tag="RD744_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557080.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5878..6435
                     /locus_tag="RD744_001063"
                     /pseudo
     CDS             5878..6435
                     /locus_tag="RD744_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6493..8532
                     /locus_tag="RD744_001064"
     CDS             6493..8532
                     /locus_tag="RD744_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDU0557081.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVIGETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(8641..9792)
                     /locus_tag="RD744_001065"
     CDS             complement(8641..9792)
                     /locus_tag="RD744_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDU0557082.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9946..10476)
                     /locus_tag="RD744_001066"
     CDS             complement(9946..10476)
                     /locus_tag="RD744_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDU0557083.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10575..10925)
                     /locus_tag="RD744_001067"
     CDS             complement(10575..10925)
                     /locus_tag="RD744_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDU0557084.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(11082..12611)
                     /gene="ahpF"
                     /locus_tag="RD744_001068"
     CDS             complement(11082..12611)
                     /gene="ahpF"
                     /locus_tag="RD744_001068"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDU0557085.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(12621..13184)
                     /gene="ahpC"
                     /locus_tag="RD744_001069"
     CDS             complement(12621..13184)
                     /gene="ahpC"
                     /locus_tag="RD744_001069"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDU0557086.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(13328..13990)
                     /locus_tag="RD744_001070"
     CDS             complement(13328..13990)
                     /locus_tag="RD744_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0557087.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            14173..15819
                     /locus_tag="RD744_001071"
     CDS             14173..15819
                     /locus_tag="RD744_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDU0557088.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            15810..16892
                     /gene="uxuA"
                     /locus_tag="RD744_001072"
     CDS             15810..16892
                     /gene="uxuA"
                     /locus_tag="RD744_001072"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDU0557089.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(17000..18553)
                     /locus_tag="RD744_001073"
                     /pseudo
     CDS             complement(17000..18553)
                     /locus_tag="RD744_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            18701..19842
                     /locus_tag="RD744_001074"
                     /pseudo
     CDS             18701..19842
                     /locus_tag="RD744_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(19968..21047)
                     /locus_tag="RD744_001075"
     CDS             complement(19968..21047)
                     /locus_tag="RD744_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0557090.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(21156..21596)
                     /locus_tag="RD744_001076"
     CDS             complement(21156..21596)
                     /locus_tag="RD744_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0557091.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(21653..22063)
                     /locus_tag="RD744_001077"
     CDS             complement(21653..22063)
                     /locus_tag="RD744_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDU0557092.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         7637 a         3924 c         4158 g         6368 t
ORIGIN      
        1 atcccagaat ttgactcgcg gaacatcttg ctagctttag tacgttatgt caattgggaa
       61 ggctatgaaa ataaaaatcc gtatgtagaa gaagcttgta acgagttgat tgattatgta
      121 gagactcatt tttttaaaag aagatattcg gaagatgaaa agtcctttat catacgagga
      181 atagagatcg caatcggaag aaaggcattt ccccttggac tgaaagaaga agataagaaa
      241 agcgcagaaa cgaagccgtt atttcaatta gtctgtcaag gattggaaga gcaaaaagga
      301 attctagact tacaagaaga agatgtctat tatatttttt ctttgttcaa cagtagaaat
      361 tataccaatg agaacatgga gctgttaaga aaagatttcg aagttgttta taaaaatttt
      421 gttctcaatg acctatcact gaatgaattg attttacaga ttattagtat cgctgaaatc
      481 gacgatgcag aaaattttat tttcaagcaa tcctttttac aatttgtccg tacgttgtgg
      541 gcagatggac aagtattttt accggaaagg atctatcttc tatcagaaaa agaacaactt
      601 ctgtacggaa aaattgtaga aatattggaa gagtggaaaa agaaaaatca aatccaacta
      661 agatggaatg aaaatttcat tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa
      721 caagcgggga gacaggaaat agaaattttt gttgtttcag aaagttcagt caaacaattt
      781 ttttatcgaa cccaatttca tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt
      841 ttatttcgtt cattagaaga attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt
      901 ttgtgcgatg ctgctagcta tcaaaatgga ttagaaactg aaaagacaaa gatctatcca
      961 atctcattaa aaggtgcaaa catccatttg tatcatattg ctcaggaact tcacctatta
     1021 aaaaaacata tttcttgatg agaccttaat taggagacct tctacaaagc tttttaattg
     1081 tctgtagaag gtttttattt gagtcctgat tcgtgcgaag atctacttat ataacaagct
     1141 ttctctgaaa tttttttaaa atgctattga cttagagtca actctaagta gtagaataga
     1201 taaagtgtaa caaatctatt ttcattaaaa gaagatagta cggaggagag aatgtattat
     1261 gagtacagta ttacatgtat tggatgcaat caatagcccc gcagatgtca aaaaattatc
     1321 tgttgaggaa atgaaacagt tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag
     1381 cgatgtaggt gggcacgttg gtccgaatct aggtgtagta gaattaacga ttgcgtttca
     1441 ttatgtattt gattcaccaa aggataaaat cgtttgggat gtttcccatc agtcttatgg
     1501 tcataagctt ttaactggtc gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag
     1561 cggatattcc aaccaacatg aaagtaaaca tgactatttt acagtggggc acacatctac
     1621 atctatcagc ttagcaacag gtttagctaa agcgagagat ctgaagaaag aatcaggaaa
     1681 tgtggtagca tttatcggtg acggctccct aagtggtggc ttagcttttg aaggattgaa
     1741 caatggtggt gttttacaat ccaatctaat cgtattagta aacgataatg agatgtctat
     1801 tgatgagaat catggcagtt tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa
     1861 aagcagcaat aacctattta aagcaatggg atttgattat cgttacgtgg ataatggaaa
     1921 cgatctggaa acggtgatcc gtttatttga agaagtaaaa gatattgatc atccgattgt
     1981 acttcacgtc catacggaaa aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa
     2041 atatcattgg catgtaccat ttgatctgtc caccggggag gcaaagcaag taacttctgg
     2101 agaaacattt aatatgatca tcatggatta tttggataag aaaatgcaaa caagtacagc
     2161 tcctattgcc gcagtcaatg cagcgatacc aggcgccttt ggactgaaaa cattccaaga
     2221 aaaacatcca gatcattatt ttgacgtggg gatcgctgaa cagcactcta tcactttcgc
     2281 ttctgctttg gcttctcagg gagtacgtcc agtcgtcttc caatcaagta ctttcttgca
     2341 acgggcatat gatcaattat cacatgattt agcgatcaac gagaatccag tgattatttt
     2401 agtcggtggc ggaacgatct cagctagtga ccgaacacac caaggaacat ttgacattcc
     2461 ttacttatca agtatcccga acatcaaata tcttgctcca gcaacaaaag aggatatgat
     2521 ctctatgcta gattgggcgt tcgaacaaac agcgcagcct gtagcaatcc gaattcctac
     2581 acatggtgta gaacatggta cagctatcgc aaatgattat agtattccag cctatcaaac
     2641 agtaaataat ggaggaaaag tagcaatttt agctttaggc ggttttcttg aattaggaaa
     2701 agaaacagta aaagctttta gaaaaaaagg aatcgaagca acgttgatca atccaggaac
     2761 gatcacagaa aatgacgtag aaacattaga taaattaaaa gaaacacacg aattagtcat
     2821 cacattagaa gatggtagtt tgtcaggagg atttggcgaa aatatcagtc gttattatgg
     2881 tgattcttca atgaacgtat taaattttgg tgcagagaaa gaattcacgg atagcgttcc
     2941 tgtagggcag ctttatgaac gttatcattt aacaccaaaa caaatcgttg aagatagtct
     3001 gcgtgtcttg aatattcaat aattcattat gctcaccatc cagataattg tttatactag
     3061 tatctggatg gttttttcat tataaaaagc aaaggggtgg atgttatgta tcagcaagta
     3121 gaagaatatg tcaaaaagtt ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt
     3181 ttcttttcat ttggggatac agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa
     3241 aagataaaga ctggtacatc aagtggttac gatctgtata aacaagagaa tgagccttta
     3301 ccaaaagttg gtcaattaga cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc
     3361 aaaaatataa atgtgaccat cttgcctttt agtgaagtca cgaaagagca tgcttggaaa
     3421 gaaggagaag gggatcggac attagcttat tggcggaaag tccatgctga gttttttaaa
     3481 catgcttata aagaagcgtt gaacatagaa tttacagaag agaagctggt cgtatacgaa
     3541 gaatttgaag taattttctt ttagtaagaa aggtctgctg aaagaaatag ctcattattg
     3601 tctcctgaat agaaaagggt tacattttta tatgaagctg tatttttata taacattact
     3661 ggaggagata ttgtggatac actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa
     3721 gctttggaaa acgagccgct acacagttct tttattatgg ggagaatggc tttggctgca
     3781 aaagaaggcg gagcagttgg tattcgagca aatacgacag aagatatcaa tgaaatcaag
     3841 cgaatcgttg atttaccggt aatagggatt atcaaaagag attacgatgg gtcagaagtg
     3901 tttattactg cgacagaaaa agaagtggaa gaacttttgg agacgcaagc agaaatcatt
     3961 gcactggatg caacttctcg taaacgtccg acagaaaaaa caactgctga acttgtccaa
     4021 atgattcacg aacacggtcg gttagctatg gcagatattt cgacttttga ggaagcgctt
     4081 caagcgcaag aagatggttt tgatcttttg tctacgactt tagcaggcta tacagaatat
     4141 tcacgaaaga cgcagacacc tgattttgat ttattgaatc aaatcataga acaagtcgat
     4201 atcccagtaa ttatggaagg acatactgac tctcctgaac aagtagagaa agcatatgaa
     4261 ttaggtgctt tttcagtcgt agttggatct attatcaccc gtccgcaatt gatcactaaa
     4321 agatatgtcg aagccgcaag taaagcaaat aactaaaaaa cacgcacggc acagattctt
     4381 gacaaaaaag aaaaattgat agatagttta aagcagattc taagaagatg aaaaaagcat
     4441 tacttttcat tttgtcttca agatctgctt tttaacgaat tttatagaaa gaaggcgaaa
     4501 ccatgtttga acggagtcct tcagaattga cagaacgtga aaattacaag ttgctgattg
     4561 gttcagtcat ccctagacca gtagcagtag taacgactaa atcagcaaaa gatatagtga
     4621 atatcgcacc cttcagttac ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg
     4681 cgatccagag aaaacaagat gcagtaaaag acacagcaaa gaatattctt gagacaagag
     4741 aagctgttat ccatatcctt gatgaggaca acgtagaaga agtaaataaa acagcagcca
     4801 atctgcctaa tgaggaaagc gaactgtacc tgactgactt gactttaact gattcagtaa
     4861 tcatttcagt accagcattg acagaagcaa aagttcgttt tgaaacatcc ttatatgaac
     4921 atgtcgagat acggaataaa aataaagtaa cagctgattt atttttgcta gaagtacaga
     4981 aatatcattt aaatgaaaca gtctatgatg ataagacagg aaggatcgat ccaagaaaat
     5041 tgaatgctgt aagccgtttg gcaggaaatg attatgcagc aataggagaa attttcacga
     5101 ttgcacgtcc cgaataaaaa ggagcactag ttatgttaaa aataagctat aaaccatcga
     5161 cagattcaaa agaaatgaaa aaagaatatg aaacagtgaa tgattttctg caagggcagt
     5221 acttagaagt tccgccattg caagaccatt ttgtcgttac tactgttact ttagatggta
     5281 aggaaataga aatgccagat caaacgatta gcggattgtt taattacttt aacaaataaa
     5341 tatttattcc gaaaagtgaa aaaacatata aaaaaacaca taaaactaaa aaattatcaa
     5401 agaatcctgg tgcaactgaa agaatttgca acgggattct ttcaacttat ttgttatttt
     5461 tttctaaaaa atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta
     5521 atcattagga aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt
     5581 gaggctccta tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa
     5641 acagaaactg ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat
     5701 agtgctaaaa ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc
     5761 tgaccttttc tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc
     5821 tgacaagtgg attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggaggt
     5881 cgctcggatt cacttctggt tgcacaatac aaccagaaaa cacaacaagc aaaactgatc
     5941 tctatcatga gagattcata tgtcgatatt ccaggttacg gaatgaataa gataaatgca
     6001 gcttactctt atgggggagt tgatttatta aatcaaacat taaaggaaaa tttcaaattt
     6061 gaagctccct attatgcaag tatcacattt caagatttta tcgattgcgt taatgaactg
     6121 tttcctggtg gagtcaagat cgatgcagaa aaatctctag atttagatgg cgtatatata
     6181 aacaaaggag agcaagtgat ggatggtaat actttactcc agtatgctcg atttcgggaa
     6241 gacaaagaag gagacttcgg gaggatcaga aggcagcagc aagtgatcaa agctatctct
     6301 cagcagctga aagacgtaac ttcaatattc aagctaccca aagcggttgg gaaattactt
     6361 ggtagtatcc aaacgaacct tccagaaagt gtgcttctgg attgtggtat ggatttcctc
     6421 aaagacatat gttagaataa acaggtataa atagtgaaat aaaggaatga caagcaagag
     6481 aaggaggaaa aaatgaaaag aagtgacaag cacggcaaaa atcgaacagg cgcttatatt
     6541 gccggcgcag tgatcttaat agcagctgcg ggtggcggtt atttttacta ccagcactat
     6601 caagaaaccc aagcagtaga agctggagaa aagacagttg agcaatttgt ccaagctttg
     6661 aacaagggag attataacaa agctgcagaa atgacatcaa aaaaggcggc aaataaaagt
     6721 gcattatctg aaaaagagat cttagataaa taccagaata tatacggtgc tgctgatgtc
     6781 aaaggacttc agatatcgaa tctaaaagta gataaaaaag acgattctac ttacagtttt
     6841 tcatataaag caaagatgaa tacctcatta ggtgaattga aagatctttc ttataaagga
     6901 acattagaca gaaatgatgg tcaaaccacg atcaactggc aacccaactt ggtttttcca
     6961 gaaatggaag gaaatgacaa agtaagcctg accacacaag aagcagcaag agggaatatt
     7021 atagatcgaa atggtgaacc attagcaaca acaggcaaac taaaacaatt aggagtcgtt
     7081 ccaagtaaac ttggagatgg aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc
     7141 ttcgacttaa cagaagatgc tatcaatcag gcgatttcac aaagctgggt acaacccgat
     7201 tactttgtcc cattgaaaat cattgatgga gcaacgccag aacttccagc tggagctacc
     7261 attcaagaag tagacggcag atattatcct ttgggtgaag cagctgctca gctgattggt
     7321 tacgtgggag atatcacagc agaagatatt gataagaatc cagaattaag cagtaatgga
     7381 aaaatcggtc gctcaggttt ggagatggct tttgataagg atcttcgtgg gacaacaggt
     7441 gggaaattaa gcattacaga tgcagacggt gtagagaaaa aagttctgat cgaacatgaa
     7501 gttcaaaatg gcaaagatat caaattgaca atcgatgcga aggcacaaaa aatagctttt
     7561 gacagtctag gaggaaaagc tgcatcaact gttgcgacaa cgccaaaaac cggtgatctt
     7621 cttgcgcttg ctagctctcc aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa
     7681 gaagattaca aagcttatga agaaaatcct gaacatccat tcatcagccg atttgcgaca
     7741 ggttatgctc ctggatctac gtttaaaatg attacagcag caatcggtct cgacaacggc
     7801 actatcgatc caaatgaagt gttgacgatc aacgggctta aatggcaaaa agacagttct
     7861 tggggatctt ttaaaacaac gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact
     7921 gctttgatct attccgataa tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa
     7981 aaatttcgta caggcttaga taaattcatt tttggtgaag accttgattt gccaattagt
     8041 atgaatccag cacaaatttc taatgaagat agctttaact cagatatctt gctagctgat
     8101 actggatatg gacagggcga acttctaatt actcctatcc agcaagcagc aatgtattct
     8161 gtttttacca acaatggcac acttgtctat cctaaattga ttgcagataa agagacaaaa
     8221 gataagaaga atgtcatcgg cgaaacagca gtacaaacga tcgtgccaga tctgagagaa
     8281 gttgtgcaag atgtaaatgg tacagcacat tctctttctg ctttagggat tccgttggca
     8341 gcgaaaactg gtacagcgga aatcaaagaa aaacaggatg taaaggggca agagaacagt
     8401 ttcttgtttg ctttcaatct tgataaccaa ggttatatga tggtcagcat gttggaaaat
     8461 aaagaagatg atgattcagc aaccaaacga gcatcggaac tattgcaata cctcaaccaa
     8521 aattatcaat aaaaaattgc tttttaagta cagtaaaagg actgtggcgt tatcgtcaca
     8581 gtcctttttt caaataaacg gttttcacga tgtctctttc acaacttctt tttgatttac
     8641 tcacacttca taaacttttt ttgtaaaata tttgagaaca agtggagaaa taagtgtact
     8701 taataaaatc acgataacta aaggtgaata gtactgtggt tcgatcaatt gactttgctg
     8761 tccaatttgt aaaatgatca aagccatttc tccacgcgaa atcataccag caccgaccat
     8821 caatgaacta tgttgagaaa aaccggcaat ctgagcacca aaataacctc caagcaattt
     8881 ggtcaaaatc gctacaagcg tcaatataga aataaagagt agttgttcac taaagtcaga
     8941 aaaatcaact tctaatccta cactgacaaa aaatactggt ataaatactg catatcctaa
     9001 agcctctaca ttgtggtata cttcttcttt aacctttgtc tgactgacag ctatcccagc
     9061 aaaaaaagca ccaatgacag agctaagccc aacaagatcc gctacgtaag acattcctaa
     9121 acaaataact agcgatacga tgataataga agaatttgcg tacacttttt ctgcaaatga
     9181 aagtaggaaa ggcgcaatcc atttgactaa taagaaaata aaaacaaaat aaattagctg
     9241 ttcaatcaaa acaagcggta aagaagcatt gctagaagct tcccccgtta gaaaagatag
     9301 gctgaaactt agaactaaaa cgactaaaat gtcatctacc acagaagcac ccaaaattgt
     9361 tgatccttct tttgtattca ctacgtttag ttcttttaat acttctactg aaatactgac
     9421 agaagttgcc gcaagaataa tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc
     9481 tttgcctgtc aaccagccaa aaacaaccgg gaagatgatc ccaagcaaag caacgaacat
     9541 accaggtcgg aaatattttt tcaaaagaga taaatcactt tccagaccag ctaaaaacat
     9601 taataagatc acaccaatct ccgaaaagtc atggactaga atatcaggtt tgacccaatt
     9661 caatccagct tggcctagca aaacaccaac aagtaattgt ccaatcactg ctgggatacc
     9721 taatctccgc gagatatgtg agccaatcgt tgttgaaatc aatatcaggc ataaaatacc
     9781 aataaattcc attctcatca ttcctttgta taaaatatag cccgaactta gcataacata
     9841 cattattgaa aaaataggaa aatatactca caaaaaaacc aacaatccaa aagatttttt
     9901 tggattgttg gtttttatac ggtctatttt aataactaaa aatgattaac gtatagtcat
     9961 ttttcttatt ctgatgaaat tgataatctc ctggatgccc atgaaaatca aaataaagcc
    10021 gaatatttgg aatagaagca tcaaactttt gaatggattg aacaataaga cgaatccgcc
    10081 aatcaacaac agaatactac tataaacact ccaactttta gcttttgtat tcatgctgaa
    10141 gaacaactgg aacaagctgt tgatgataac agctaatcca agaagaatcg gcaggataga
    10201 aacgattgca ggtgcaaaag catagacgat cagagctaag atcaggaaaa aaagaccact
    10261 gaacagaccg atactccaag aacctgtttt ccttttaaaa cggtaagcct caaaaagatt
    10321 caacaaccca agcaaaacaa gataggcggt gatcagataa ccgataaaat gaaaaacagc
    10381 agaagggttt agcacaatcg caatcccaga aataatataa acagcagctc ttaataaggc
    10441 gtatttttga atattttgtt taatcgtctc aaacatataa gaaaacctcc tttgacttat
    10501 tttaacgtga actagtcatt ttaccaaatt aaatgttaca aaatgagcga aggagaagaa
    10561 tatctctgga aaaatcaact tctgaggatc ttttccattt ttttgccttt tgctagctca
    10621 tcgatcattt tatctaaata acgaatttct ttcatcaagt tatcctcgat gtcttctact
    10681 cgtacaccac aaacaacacc tgtaatcaag gaacgtgaag gattggggac aggggcttcc
    10741 gcaaaaaatt gttcgaaagt cacttgtttt tgtagttgat attctagttc tgtttgggaa
    10801 taacccgtca gccaacaaat gatttcatct acttcctctt ttatacgatc ttttctctcc
    10861 gcttttgcta catacatagg ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc
    10921 gccataatca gtcacctttc tttttttcaa attatttact acttatagta tataagaaaa
    10981 agagaagaaa aagcgattat tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc
    11041 atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg catcagataa
    11101 tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat ttgtttgtat
    11161 ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc gccatttttc
    11221 tcggtcacta tttcatttct tggattcatt tcgatcgttc tatctagcca atccgtatta
    11281 gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat ctccatcgta
    11341 cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc tttcgtcgct
    11401 gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa gatttgatct
    11461 gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc agccaaatcg
    11521 attgctgctt caacaccaga attaccacca ccgataacag caatctcttt tcctttaaat
    11581 aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc tttttcgcct
    11641 ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt
    11701 gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa ggaaacggcc
    11761 tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac ttgctgcatc
    11821 aattttggac cttctgtgta agaggtacca atcatatttt caatccctag tgtttcaacg
    11881 acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg tgctgcatag
    11941 atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata aggttctttt
    12001 tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg ttcgatcgtc
    12061 atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc catgattttt
    12121 ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt aatatttgga
    12181 tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg acaagtcaag
    12241 ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag actcaaagat
    12301 tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa agaggaaaat
    12361 tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac tggattgata
    12421 gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg agacatttct
    12481 gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc caaactaaca
    12541 gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg tttagtttct
    12601 ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt
    12661 tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg tacatattga
    12721 gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc tgcattgatt
    12781 tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg agctaatcct
    12841 tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc accgatcatc
    12901 acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg agtaaagtgg
    12961 gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact gtattgttct
    13021 tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata gaagcaaaga
    13081 atactccagt tccccataag atctttttca ctgacttcta aaaattctcc ttgatgataa
    13141 gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa acctccaata
    13201 ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa tggatatttt
    13261 gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa gaggtcgcaa
    13321 gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc tgttctttca
    13381 gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt ttatcaacta
    13441 aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc ccctgaatgg
    13501 tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac cagttccaaa
    13561 cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct gcacggaaaa
    13621 aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc gtttctagca
    13681 ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc tcttccaact
    13741 gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa acatacgtac
    13801 cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat gcttcgcgaa
    13861 tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt ttccttccag
    13921 gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt gattgcaagc
    13981 tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac agaagaggtc
    14041 tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt aaaattttgg
    14101 ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa tatgttagtt
    14161 taggagggaa aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa
    14221 aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat
    14281 cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct
    14341 caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat
    14401 gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg
    14461 ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt
    14521 catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta
    14581 caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa
    14641 tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg
    14701 agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc gattgcactt
    14761 gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc
    14821 gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat
    14881 gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg
    14941 tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca
    15001 aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa
    15061 gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt
    15121 gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg
    15181 tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat
    15241 ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag
    15301 tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct
    15361 aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca aaaggtggcg
    15421 attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca
    15481 cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat
    15541 caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca gcataagctt
    15601 tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt
    15661 ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata
    15721 gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac attacaggat
    15781 tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac
    15841 aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag
    15901 gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc aaagaactaa
    15961 aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg
    16021 atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa
    16081 tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg
    16141 actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta
    16201 tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca
    16261 atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa tccttgcttg
    16321 aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat
    16381 caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc
    16441 catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta gattggttgt
    16501 gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt attgcagaag
    16561 atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat cgaatcccat
    16621 ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccacata
    16681 aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat
    16741 ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag
    16801 gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa
    16861 tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa
    16921 ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga
    16981 tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga
    17041 gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt
    17101 cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt
    17161 tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga
    17221 tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat
    17281 cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat
    17341 gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt
    17401 gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca
    17461 atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt
    17521 taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt
    17581 tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct cttgaataaa
    17641 gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg
    17701 attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac
    17761 aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag tcaatatatt
    17821 catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac
    17881 atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt
    17941 cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt
    18001 ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta
    18061 cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtact
    18121 atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg
    18181 tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct
    18241 gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat
    18301 caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta
    18361 gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag
    18421 acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga
    18481 aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt ttaatcgctt
    18541 cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac
    18601 tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc
    18661 ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt
    18721 aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga
    18781 ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt
    18841 tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc
    18901 gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac aatcagctgt
    18961 tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca ctttgactat
    19021 ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc
    19081 agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt taacgccgta
    19141 tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag atcttgttga
    19201 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    19261 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    19321 agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag atgtaactcc
    19381 agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag acacagataa
    19441 tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag atgtgactcc
    19501 agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca aaaaaacaat
    19561 tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa aaataaatga
    19621 agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg aaaccttaaa
    19681 aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct accaaaatcc
    19741 ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag tatagcaaca
    19801 atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa taatgtctgt
    19861 gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata ccagattgaa
    19921 atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta agggatatct
    19981 gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa aaagaaggta
    20041 aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct aacaagtaag
    20101 accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt cggattcagc
    20161 acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac aacatctcgg
    20221 atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat aacacctgct
    20281 actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg atgaaagcca
    20341 aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac aaattgagct
    20401 tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat atcttgaaag
    20461 aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt tttcttttca
    20521 atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat ttttcctata
    20581 tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa aaaatcgttt
    20641 cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg ctgatgctcg
    20701 taaaaatcaa taacggactg gatcatatca aatgtttgat gaataagcat cgggatatac
    20761 tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat agctaaaaca
    20821 cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat caaataagta
    20881 aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag aacaaaaacg
    20941 acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg ctgataaacg
    21001 gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat atgatggaat
    21061 atgaaaataa gaaagagaaa gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag
    21121 ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg gtcttgatga
    21181 aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt
    21241 ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa cgcagcacct
    21301 tgaggagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg acgattaata
    21361 taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc cagacacaaa
    21421 tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa catcacaaga
    21481 tactgggtat aggtaagatt cagcggcttg aggagggaag tgtagtgtct aatgatttcc
    21541 ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc ttccattttt
    21601 ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa gtctattcac
    21661 taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac ggacacactt
    21721 tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg atagatcctt
    21781 caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa acttgtgcac
    21841 tgacagtaga agccccagag atattttttt gctgtttgat caattctaat gcactgttaa
    21901 aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt
    21961 taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgaact tctccagagc
    22021 gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta ccacctttaa
    22081 ttatatt
//
LOCUS       DAPCMU010000035        21988 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPCMU010000035 DAPCMU010000000
VERSION     DAPCMU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.035"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..395
                     /locus_tag="RD744_001078"
     CDS             <1..395
                     /locus_tag="RD744_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557093.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="RD744_001079"
     CDS             398..1147
                     /locus_tag="RD744_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557094.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="RD744_001080"
     CDS             1159..1428
                     /locus_tag="RD744_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557095.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="RD744_001081"
     CDS             1433..1597
                     /locus_tag="RD744_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557096.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="RD744_001082"
     CDS             1590..1892
                     /locus_tag="RD744_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557097.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="RD744_001083"
     CDS             1889..2050
                     /locus_tag="RD744_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDU0557098.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="RD744_001084"
     CDS             2047..2352
                     /locus_tag="RD744_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557099.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="RD744_001085"
     CDS             2352..2708
                     /locus_tag="RD744_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDU0557100.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="RD744_001086"
     CDS             2695..2913
                     /locus_tag="RD744_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557101.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="RD744_001087"
     CDS             2910..3329
                     /locus_tag="RD744_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDU0557102.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="RD744_001088"
     CDS             3326..3883
                     /locus_tag="RD744_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDU0557103.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="RD744_001089"
     CDS             3880..4176
                     /locus_tag="RD744_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557104.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="RD744_001090"
     CDS             4253..4666
                     /locus_tag="RD744_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDU0557105.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="RD744_001091"
     CDS             5012..5386
                     /locus_tag="RD744_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557106.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="RD744_001092"
     CDS             5423..5590
                     /locus_tag="RD744_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557107.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="RD744_001093"
     CDS             5616..5960
                     /locus_tag="RD744_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDU0557108.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="RD744_001094"
     CDS             5965..6246
                     /locus_tag="RD744_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557109.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="RD744_001095"
     CDS             6349..6663
                     /locus_tag="RD744_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDU0557110.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="RD744_001096"
     CDS             6641..8335
                     /locus_tag="RD744_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDU0557111.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="RD744_001097"
     CDS             8355..9533
                     /locus_tag="RD744_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDU0557112.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="RD744_001098"
     CDS             9496..10182
                     /locus_tag="RD744_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDU0557113.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="RD744_001099"
     CDS             10182..11342
                     /locus_tag="RD744_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDU0557114.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="RD744_001100"
     CDS             11352..12227
                     /locus_tag="RD744_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557115.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="RD744_001101"
     CDS             12224..12535
                     /locus_tag="RD744_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557116.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="RD744_001102"
     CDS             12525..12878
                     /locus_tag="RD744_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDU0557117.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="RD744_001103"
     CDS             12868..13269
                     /locus_tag="RD744_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557118.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="RD744_001104"
     CDS             13262..13666
                     /locus_tag="RD744_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557119.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="RD744_001105"
     CDS             13678..14286
                     /locus_tag="RD744_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0557120.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="RD744_001106"
     CDS             14306..14668
                     /locus_tag="RD744_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557121.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="RD744_001107"
     CDS             14671..14853
                     /locus_tag="RD744_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557122.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="RD744_001108"
     CDS             14870..18289
                     /locus_tag="RD744_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDU0557123.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="RD744_001109"
     CDS             18340..19077
                     /locus_tag="RD744_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0557124.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="RD744_001110"
     CDS             19087..21378
                     /locus_tag="RD744_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0557125.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="RD744_001111"
     CDS             21402..>21988
                     /locus_tag="RD744_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557126.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAPCMU010000036        21910 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPCMU010000036 DAPCMU010000000
VERSION     DAPCMU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.036"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD744_001112"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="RD744_001112"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDU0557127.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="RD744_001113"
     CDS             complement(1172..1867)
                     /locus_tag="RD744_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDU0557128.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="RD744_001114"
     CDS             complement(1880..2596)
                     /locus_tag="RD744_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDU0557129.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="RD744_001115"
     CDS             2926..3534
                     /locus_tag="RD744_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDU0557130.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="RD744_001116"
     CDS             3859..4182
                     /locus_tag="RD744_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDU0557131.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="RD744_001117"
     CDS             4364..5734
                     /locus_tag="RD744_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDU0557132.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="RD744_001118"
     CDS             complement(5864..7165)
                     /locus_tag="RD744_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDU0557133.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="RD744_001119"
     CDS             complement(7519..9477)
                     /locus_tag="RD744_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDU0557134.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="RD744_001120"
     CDS             complement(9654..9920)
                     /locus_tag="RD744_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0557135.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="RD744_001121"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="RD744_001121"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDU0557136.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="RD744_001122"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="RD744_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDU0557137.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="RD744_001123"
     CDS             13264..13827
                     /locus_tag="RD744_001123"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDU0557138.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="RD744_001124"
     CDS             complement(14080..14601)
                     /locus_tag="RD744_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDU0557139.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="RD744_001125"
     CDS             complement(14604..15446)
                     /locus_tag="RD744_001125"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDU0557140.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="RD744_001126"
     CDS             complement(15439..16164)
                     /locus_tag="RD744_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDU0557141.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="RD744_001127"
     CDS             16340..16636
                     /locus_tag="RD744_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557142.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="RD744_001128"
     CDS             17204..18796
                     /locus_tag="RD744_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDU0557143.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="RD744_001129"
     CDS             18809..19549
                     /locus_tag="RD744_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0557144.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="RD744_001130"
     CDS             19717..21066
                     /locus_tag="RD744_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0557145.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="RD744_001131"
     CDS             complement(21194..21784)
                     /locus_tag="RD744_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDU0557146.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6293 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa
//
LOCUS       DAPCMU010000037        21809 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPCMU010000037 DAPCMU010000000
VERSION     DAPCMU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.037"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            29..583
                     /locus_tag="RD744_001132"
     CDS             29..583
                     /locus_tag="RD744_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0557147.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(756..1229)
                     /locus_tag="RD744_001133"
     CDS             complement(756..1229)
                     /locus_tag="RD744_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0557148.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1241..1777
                     /locus_tag="RD744_001134"
     CDS             1241..1777
                     /locus_tag="RD744_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0557149.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1817..2188
                     /locus_tag="RD744_001135"
     CDS             1817..2188
                     /locus_tag="RD744_001135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0557150.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2472..3743
                     /locus_tag="RD744_001136"
     CDS             2472..3743
                     /locus_tag="RD744_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0557151.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3988..5013
                     /locus_tag="RD744_001137"
     CDS             3988..5013
                     /locus_tag="RD744_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0557152.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5307..6416
                     /locus_tag="RD744_001138"
     CDS             5307..6416
                     /locus_tag="RD744_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDU0557153.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6499..7395
                     /locus_tag="RD744_001139"
                     /pseudo
     CDS             6499..7395
                     /locus_tag="RD744_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7411..8235
                     /locus_tag="RD744_001140"
     CDS             7411..8235
                     /locus_tag="RD744_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0557154.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8267..9577
                     /locus_tag="RD744_001141"
     CDS             8267..9577
                     /locus_tag="RD744_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDU0557155.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9725..10984
                     /locus_tag="RD744_001142"
     CDS             9725..10984
                     /locus_tag="RD744_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0557156.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10994..11866
                     /locus_tag="RD744_001143"
     CDS             10994..11866
                     /locus_tag="RD744_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557157.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11878..12708
                     /locus_tag="RD744_001144"
     CDS             11878..12708
                     /locus_tag="RD744_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0557158.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12748..14931
                     /locus_tag="RD744_001145"
     CDS             12748..14931
                     /locus_tag="RD744_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDU0557159.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14992..16212
                     /locus_tag="RD744_001146"
     CDS             14992..16212
                     /locus_tag="RD744_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDU0557160.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16209..17669
                     /gene="gtfA"
                     /locus_tag="RD744_001147"
     CDS             16209..17669
                     /gene="gtfA"
                     /locus_tag="RD744_001147"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDU0557161.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17746..18852
                     /locus_tag="RD744_001148"
                     /pseudo
     CDS             17746..18852
                     /locus_tag="RD744_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19174..19383
                     /locus_tag="RD744_001149"
     CDS             19174..19383
                     /locus_tag="RD744_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0557162.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19376..19525
                     /locus_tag="RD744_001150"
     CDS             19376..19525
                     /locus_tag="RD744_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDU0557163.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19679..20344
                     /locus_tag="RD744_001151"
     CDS             19679..20344
                     /locus_tag="RD744_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0557164.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20628..21713)
                     /gene="xerS"
                     /locus_tag="RD744_001152"
     CDS             complement(20628..21713)
                     /gene="xerS"
                     /locus_tag="RD744_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDU0557165.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7404 a         3290 c         4205 g         6910 t
ORIGIN      
        1 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
       61 tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg aaagagatag
      121 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
      181 agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa caggtaagcg
      241 aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg aaggtaaaga
      301 agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa gggatcgtgt
      361 ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag gaacacatac
      421 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
      481 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
      541 tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa atttttataa
      601 acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt cataaagttc
      661 gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat aaactaagta
      721 attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat caatacggtc
      781 tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt tagcagacta
      841 tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgacctt ctgcaaactt
      901 ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt ttcctctgtc
      961 tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc tgtatgtcca
     1021 agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact acaatgggta
     1081 tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat gattttcaaa
     1141 ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa caaaagttgt
     1201 tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac gtattcatca
     1261 catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata atatactcgg
     1321 tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc acctgttttt
     1381 ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac aagaagggaa
     1441 caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc cttccgaagc
     1501 atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt atggaatcga
     1561 atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac gattagcttt
     1621 agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca cagaccagat
     1681 ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac aatattccga
     1741 tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg tcagtttctt
     1801 ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct atcaagaagt
     1861 tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta gcgtccatca
     1921 tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac aattacaaga
     1981 gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac tttattatcg
     2041 tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag atgcaaaagc
     2101 gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc agtacttaaa
     2161 tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg aagagtaaga
     2221 ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta aagaatgttg
     2281 atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac tacttatgag
     2341 tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa acatctgcca
     2401 aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg agtaccaaat
     2461 gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc agtctcaaaa
     2521 ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa tcatcgattg
     2581 tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat gatagtttta
     2641 cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat aatctttcta
     2701 cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg ttagggacga
     2761 ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca cctagtttag
     2821 gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc tgtcagggaa
     2881 tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt gataatgctg
     2941 attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag gaacaggcaa
     3001 agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat gttaaaacta
     3061 ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta aaagaatttt
     3121 tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat ctttcaaaag
     3181 agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca aatgattacc
     3241 aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa atagcgtttc
     3301 tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat caagcttata
     3361 tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat aacaactctg
     3421 aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt actggagatt
     3481 atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg atggaacaat
     3541 tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct gcgttgacaa
     3601 caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg attggactgg
     3661 ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg atttctcgag
     3721 actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta acaataaaat
     3781 atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa attcactaga
     3841 attagcatta tttactctaa attgttcgga tattttttct tgtttttaat tatgattaca
     3901 gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg ttagtaaaca
     3961 ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc aaaacgggcg
     4021 ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact ttcagtaacc
     4081 gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt caaaaaaagt
     4141 cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga agtagatgag
     4201 ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc agaacaaagc
     4261 aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca agacgatttg
     4321 ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat tgaagaagta
     4381 tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc tgaagtagat
     4441 gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt gtataaaaaa
     4501 ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag ccaaggaaat
     4561 aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat gaaacaaaaa
     4621 ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga cggaatgcag
     4681 gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc tatcatggta
     4741 ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg tctacatatt
     4801 ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt tttaacacct
     4861 gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt tcgcatggtt
     4921 aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc taataatatt
     4981 attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa aaagggtgtt
     5041 tacttttttg tttactaata tttttagtaa aaaatctcga caaaacatta agtaaacagt
     5101 tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag atgaaacatt
     5161 tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc gcagcatgtg
     5221 gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg agttgagata
     5281 ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa aatcctgata
     5341 tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta gaagctgcaa
     5401 caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc tataaatata
     5461 tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat attgacatgg
     5521 gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta tacgcaatcc
     5581 ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat aatgcaggtg
     5641 tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca attgatttaa
     5701 cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag actggctact
     5761 ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca aaatcaggtt
     5821 tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca cttgttgatc
     5881 atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt tttcaatcag
     5941 gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg aaaaatgact
     6001 atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat gcaacaatta
     6061 caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa gtgaaaaaac
     6121 taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg ggggcatcaa
     6181 tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca attgatttga
     6241 gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa gatggattac
     6301 gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa gtaactgttg
     6361 aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga aattaaatta
     6421 gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta atggatgaat
     6481 accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa agatcaagtg
     6541 atatggggct atatttttat ttttccaacg gcattaggat taattttatt aaatatatgg
     6601 ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg tgatactgaa
     6661 tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt tcagtcttta
     6721 ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt atccttgttg
     6781 gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac aatttatttt
     6841 ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat gttcaataat
     6901 gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc agcatggtta
     6961 acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag tgctattggt
     7021 tataacatgg tgttattact agccggctta taagaaattc ctaaagatta ttatgaagca
     7081 gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt accattagtt
     7141 acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca agtatttgat
     7201 gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca atcattagcc
     7261 tatttattct ataaacattc atttatttta aatgacaagg gatatggttc ggcaatcgtt
     7321 atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag
     7381 tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca
     7441 atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt
     7501 ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag
     7561 aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat
     7621 atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg
     7681 gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta
     7741 cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa
     7801 aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt
     7861 ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc
     7921 tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa
     7981 tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg
     8041 ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta
     8101 caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg
     8161 ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca
     8221 ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac
     8281 atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac
     8341 tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga
     8401 atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt
     8461 ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca
     8521 agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt
     8581 gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact
     8641 accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt
     8701 gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa
     8761 gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg
     8821 aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat
     8881 ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt
     8941 agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt
     9001 tggttactat attacagaat caagtgaaca caatgcagaa taccatccat actttattaa
     9061 atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg
     9121 ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga
     9181 gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg
     9241 aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac
     9301 agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt
     9361 cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact
     9421 gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga
     9481 tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat
     9541 cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa
     9601 ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag
     9661 taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca
     9721 taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc
     9781 tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt
     9841 taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa
     9901 aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac
     9961 acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt
    10021 gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa
    10081 tatcaaaaat ggatatgcac aacgttttgc tattgaggat cgtgtatata gcataccatt
    10141 aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca
    10201 agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa
    10261 agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc
    10321 tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt
    10381 taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt
    10441 aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt
    10501 tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat
    10561 tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg
    10621 tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca
    10681 taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata
    10741 ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc
    10801 accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa
    10861 agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa
    10921 tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag
    10981 gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt
    11041 agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt
    11101 ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa
    11161 ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt
    11221 gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa
    11281 cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc
    11341 gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg
    11401 tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat
    11461 ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt
    11521 agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa
    11581 tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt
    11641 atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg
    11701 tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa
    11761 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
    11821 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
    11881 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
    11941 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
    12001 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
    12061 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
    12121 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
    12181 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
    12241 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
    12301 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
    12361 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
    12421 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
    12481 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
    12541 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
    12601 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
    12661 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
    12721 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
    12781 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
    12841 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
    12901 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
    12961 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
    13021 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
    13081 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
    13141 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
    13201 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
    13261 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
    13321 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
    13381 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
    13441 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
    13501 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
    13561 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
    13621 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
    13681 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
    13741 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
    13801 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
    13861 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
    13921 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
    13981 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
    14041 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
    14101 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
    14161 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
    14221 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
    14281 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
    14341 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
    14401 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
    14461 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
    14521 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
    14581 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
    14641 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
    14701 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
    14761 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
    14821 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
    14881 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
    14941 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
    15001 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
    15061 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
    15121 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
    15181 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
    15241 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
    15301 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
    15361 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
    15421 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
    15481 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
    15541 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
    15601 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
    15661 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
    15721 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
    15781 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
    15841 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
    15901 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
    15961 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
    16021 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
    16081 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
    16141 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
    16201 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
    16261 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
    16321 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
    16381 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
    16441 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
    16501 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
    16561 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
    16621 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
    16681 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
    16741 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
    16801 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
    16861 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
    16921 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
    16981 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
    17041 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
    17101 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
    17161 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
    17221 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
    17281 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
    17341 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
    17401 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
    17461 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
    17521 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
    17581 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
    17641 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
    17701 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
    17761 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
    17821 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
    17881 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
    17941 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
    18001 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
    18061 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
    18121 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
    18181 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
    18241 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
    18301 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
    18361 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
    18421 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
    18481 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
    18541 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
    18601 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
    18661 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
    18721 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
    18781 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
    18841 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
    18901 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
    18961 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
    19021 tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata caaagattta
    19081 taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta gtagtgttct
    19141 tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa aaatgaatta
    19201 atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc aaaagcaatt
    19261 atggtttcaa aaagctatac ctattacgaa aatagaagat taggactagt acctgttgaa
    19321 tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca gaatcatggt
    19381 taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg gattcgacaa
    19441 acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa agaagaaaaa
    19501 aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct caaagtacta
    19561 tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg aaagtagaag
    19621 aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt tctttaaaat
    19681 ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt tattgagcaa
    19741 atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat tagatcgagc
    19801 agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag
    19861 gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt caacttttta
    19921 tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt ttatgaccgt
    19981 tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg aaaataaaaa
    20041 cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt ttgtagaacc
    20101 cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta cctttttaaa
    20161 agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt cttataatgg
    20221 aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa cgatcgttgg
    20281 tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc ttatatcaca
    20341 ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac aaacgacttt
    20401 aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg atcccacaga
    20461 ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata ataaaaaata
    20521 aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact ataatacagg
    20581 cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca taaagcattt
    20641 aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt attggtactt
    20701 gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta gagtctagta
    20761 gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt tgaatatttt
    20821 gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg gtaaattgct
    20881 agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat agctagataa
    20941 ttagctaaat attccattgc gaatgctgct atattgacag catctctttt atttcctttt
    21001 ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat atttataacc
    21061 tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc tctttctaca
    21121 tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc attggcaata
    21181 taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat ggcattggca
    21241 cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg attattgtct
    21301 aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa tagaaccttt
    21361 aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg
    21421 agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt caaatgagat
    21481 ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga agtatcagga
    21541 acgatagact cactcagaag ccattcataa aagagcttat aattttttag atattctaaa
    21601 gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg
    21661 ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt catgtcattc
    21721 atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc aattatagaa
    21781 tgttttgtca attggtatgc tctaaaatt
//
LOCUS       DAPCMU010000038        21214 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPCMU010000038 DAPCMU010000000
VERSION     DAPCMU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.038"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            29..256
                     /locus_tag="RD744_001153"
     CDS             29..256
                     /locus_tag="RD744_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557166.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            321..893
                     /locus_tag="RD744_001154"
     CDS             321..893
                     /locus_tag="RD744_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557167.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            894..1205
                     /locus_tag="RD744_001155"
     CDS             894..1205
                     /locus_tag="RD744_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDU0557168.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1196..1456
                     /locus_tag="RD744_001156"
     CDS             1196..1456
                     /locus_tag="RD744_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557169.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            1617..1976
                     /locus_tag="RD744_001157"
     CDS             1617..1976
                     /locus_tag="RD744_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557170.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            1973..3616
                     /locus_tag="RD744_001158"
     CDS             1973..3616
                     /locus_tag="RD744_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDU0557171.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            3651..4733
                     /locus_tag="RD744_001159"
     CDS             3651..4733
                     /locus_tag="RD744_001159"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557172.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            4777..5949
                     /locus_tag="RD744_001160"
     CDS             4777..5949
                     /locus_tag="RD744_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDU0557173.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            5936..6640
                     /locus_tag="RD744_001161"
     CDS             5936..6640
                     /locus_tag="RD744_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDU0557174.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            6645..7805
                     /locus_tag="RD744_001162"
     CDS             6645..7805
                     /locus_tag="RD744_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDU0557175.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            7881..8159
                     /locus_tag="RD744_001163"
     CDS             7881..8159
                     /locus_tag="RD744_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDU0557176.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8140..8511
                     /locus_tag="RD744_001164"
     CDS             8140..8511
                     /locus_tag="RD744_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDU0557177.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8527..8931
                     /locus_tag="RD744_001165"
     CDS             8527..8931
                     /locus_tag="RD744_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557178.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            8928..9338
                     /locus_tag="RD744_001166"
     CDS             8928..9338
                     /locus_tag="RD744_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557179.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9399..9986
                     /locus_tag="RD744_001167"
     CDS             9399..9986
                     /locus_tag="RD744_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDU0557180.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10059..10526
                     /locus_tag="RD744_001168"
     CDS             10059..10526
                     /locus_tag="RD744_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDU0557181.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10572..10880
                     /locus_tag="RD744_001169"
     CDS             10572..10880
                     /locus_tag="RD744_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557182.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11075..17809
                     /locus_tag="RD744_001170"
     CDS             11075..17809
                     /locus_tag="RD744_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDU0557183.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            17813..18685
                     /locus_tag="RD744_001171"
     CDS             17813..18685
                     /locus_tag="RD744_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDU0557184.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            18682..19731
                     /locus_tag="RD744_001172"
     CDS             18682..19731
                     /locus_tag="RD744_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557185.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            19735..20559
                     /locus_tag="RD744_001173"
     CDS             19735..20559
                     /locus_tag="RD744_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557186.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20577..>21214
                     /locus_tag="RD744_001174"
     CDS             20577..>21214
                     /locus_tag="RD744_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDU0557187.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7625 a         3171 c         4488 g         5930 t
ORIGIN      
        1 tttttcttta cataaaggag gctgcataat gagaaactac tggtatgtat cactaactaa
       61 tgaatatcct cgaaccattg gtgattgttc agtgcgtgtt gtgcgttctg tacaaatcaa
      121 agggaagtac tctattgtcg aaatgctaag agaagctaca ccaaacgaag tggataaatg
      181 caagctgata tattgcggtc atggctattg gaaagacgag tatatccaat acaacattga
      241 gaggtacatc aaatgaatga taactatgat tacatcaagt tgttagcaag taattattag
      301 atgtttttag gaggaaaact atgaaattaa aagattatat cagagaaggg tataacgttg
      361 taactgcacc caatctagct tacaaaattc aagaagatta tcccaatgct ttagtgttta
      421 ctgatagagc tttagatgcc attcctatag ggaagttatt agtagatcgt ttttatagca
      481 ataatccagc tgttcttagt gctaagccac ttcaaaacgc ttatgctatt gaacgcttta
      541 cttgcgagtt tactggatat gagacggtag taccaatgac aggtgagaca ggaagaaaag
      601 taattcaaca gatcaaaaag aggattgctg aattgacgtt agatgatgga agtaatacga
      661 accttctaga aatcaaacta agagatactg actcagtacc agaagtttat tataaaggtc
      721 aacgaatgga tgagatgccg ataggtcttg tcgatattac ttatcattgg gatacggaag
      781 aatatgaatg ccaagtacca aaaggagcta acgacatcac tattgaatat tatgattcac
      841 ataataacaa gtacttagat agaaaaatca ttgggcacaa gagagatatg taaatgattg
      901 atgtttcaag taaacaagca agagctaggt tctatggatc aactgagtgg aggaaactta
      961 gaagtcaatg cttagagcga gatcattatg agtgccaatg gtgtaaagca gagggtagac
     1021 taacaactca acatgattca attcttgaag tggatcatat aaaggagctg gagtatcatc
     1081 cggatttagc tctagatata aacaacctaa gaaccttatg caaagattgc cataacaaaa
     1141 ggcacaaccg tatgaaatat cgtggccaac aaaagaaaag aaagtgggat gatgaatggt
     1201 ggtaagaaaa acagcaaaca gagatgatgt caaacgagca gaagcaatcg ctatggttga
     1261 taaggctatt gataagttgg ttctatttaa gagtcagctc attgaaggaa caacaactgt
     1321 agaggatata tctatcaaca atagactaat aggtaacgct aatgaagtaa cagggacatt
     1381 taatattaat gtagacattg attatacctt gagtccattt gcacatattg tagaaagcaa
     1441 cgagcaagac ggctgataaa tcagtataaa gcgaagatat atagagtgaa atacccccgg
     1501 tcaaaagatt ttgagaagaa ataaaatttt tgggaaccgg tggatggggt caactctgca
     1561 aataaatgat gctattttca cacgaccccc tccccacgta gaaaggaagt gagttgatgg
     1621 ctgatctgca agagagaaat aggcttgttt ctgctgaaga aaggcgtcta aataaattgt
     1681 ttaaggatat tgataaggat aaaaagaagg tcgtttctgg tttgattacg caagcggcac
     1741 gcttaaaaat tcttcttgat gaaatgtgga ttgatatttc cgaaaaaggt gattatgagc
     1801 ttttctctca gtcagaaaat caaaaacctt acgagcggga acgaccagtt gctaaacagt
     1861 ataattcgag agaccaagca tatcaacgtg taattaagca acttactgac tatttgccag
     1921 aagaaaagcg cgaacctaca aaaaatgcag cgctagatgg tagtgatttg ctgtgacaac
     1981 attaaagcca ttctattttg atgaatacgt agatttatac gaaaaagggg taatcccttt
     2041 taataaagaa agaattcagt taattactta tttaaaaaac gaagtgctag ttcgggatga
     2101 tatctatttt gatgaagaaa tgattcaaaa gtttatcaag tacactgaaa aaaatttttt
     2161 tcctttagct aaatatcaaa aatttatcac acctttcatt ttctgctatc aaaaagagga
     2221 cgacgaggtg ttttttgatg aaattctaaa tagtattgcg cgaggcggcg ggaaaaatgg
     2281 gttcatgtca gcaagagact cattttttat tagccctctc tacggtgtca gaaattatga
     2341 tgtaacgatt acagcgaatt ctgagaagca gggaaaggta tcttttaaag aagtttatga
     2401 aatggtacaa atgcgcaaac tggaaaaaca attttatttg actaagatgg ctatagtcaa
     2461 caatgtaact aattcgattt ttagttatag gacaaacaat cctaagacta tggatagtgc
     2521 aagagatggc tgcttagaat ttgatgaaat ccatcagttt gaatcttcag acctagttga
     2581 tattcagcgt agtgggctag ggaaaataaa acatcctcga actttttata atggtacaaa
     2641 tggtcatgtt agagaaggtt tttatgacaa aatgttggaa cgagcacaaa agatttttag
     2701 tggagagaac aagaatgatc gcttgtttcc ctttatttgt aaactggata acattgatga
     2761 aatggataat cctaaaatgt ggtcaaaggc taatccaatg ttcgaagaag attcagcata
     2821 ttcaaaacgt ctgttttcta cagtgatgaa agaatataaa aaactagaag atgagccatc
     2881 aggtaggcgt gaatttgttg ttaaacgaat gaattttaca gaaggtgatg cagaatcaga
     2941 tattacgact catgaaaaat taatggctac taaacaagaa ttgccaaatt tgaatggtcg
     3001 ttcttgtgtg gctggatttg attatgcaag tattcgtgat tttgcaacag tgggtttgct
     3061 ttttaaaatt gatgataaat ttatctggca gcaacatagt tttgctagga aaaagtttct
     3121 agatacattc aaactgaaag cgcctattaa ggattgggag aaaaaaggtt tagtgacaat
     3181 cgttgatgag ccatcaattg atcctcgtca tttaattgat tggttaaatg aaaaaagaaa
     3241 aatccatcaa atagaaatcg tttgtgccga cggatttcgt atggatttgc tgaaacctct
     3301 tctagagaaa gaatcatata attttgagtt tatcagaaat ataagaggtg tgcagtcaaa
     3361 agttgctcct attattgaag atggttttgc aaatgaacgg tttatttttg gtgatgatcc
     3421 tatgatgcgt tggtatacaa ataattcgtt tatcaaagaa gacaaattag ggaataaaac
     3481 atttttgaaa aaagagccag taaggcgtaa aacagatggg tttcatgctt ttttggcagc
     3541 tttatataaa cgagaagaaa taactgatat ggattatgag gaagcatttg atatgttgga
     3601 tgagattgaa ttttaaggta tactccttat tgaaaggggt agttatagaa gtgaaaaaga
     3661 gatattttct atttgttaca atatttataa taatagcaat ttgtgttttg acagtatttt
     3721 taccacaaat tactgagtat ttaaattatc ggatatttac cacaaaagta gatgatagcg
     3781 ttactagttt aagtaggcta gaatttataa aaatatgtgc atctgtgttt ggtcctattt
     3841 tgactgtaat ggtatttgtg aatactttaa atatgcagaa aaaaacagag caaaagcaaa
     3901 atgagcttga agtaaagcaa gctaaggctg agaacgagag agcaaaaaaa gagctgttaa
     3961 cacagataga tcgtgagttc tattttttat taaatatgtt tattgaaata caaaaagatc
     4021 aaaatgccaa acaagctgtt attgctttac agaagttggc aatttttaac gaaaagggtt
     4081 ttaatcaaga ctatgcattt acttctaaag atttatcaga tagtgaaatt gggagtacaa
     4141 ttgaacaatc aggaccgctg aagggcacag ttgaatatat aaaatttgaa acatttgatg
     4201 taagcaagtc gatggggaat tatcaaacaa aaacaaaaga cgttgctaca agtttacacg
     4261 atcagaaagt agctatattg aatgaacaat atgaaaagat gcaaatatat cttggacggt
     4321 acttcaaaat gtttcaccga atagttaaaa cattaaatga gtattatgac gattataatg
     4381 attttgatgt gaagaggtat acaaaatata tagggacatt aagaacacaa atttctccag
     4441 ctgaattcca agtgatttta ttcaattctc tatatataaa acgaggtttt ggcttaggaa
     4501 ttcaacttat tggaagtggt ttttttggag atgattttga ttttgaaact aatcaacatt
     4561 ttgaaacaag tatcaatgaa cagtggtttc tatcactatc aactgttgac agtgataact
     4621 ctataaagcg acaaaaatta tctgaatata ttaaagaatt aggatccgta gaatataaaa
     4681 aaatagctaa ttttgaaagt ttatataaac tctttaatga aactaagctc tagaaatagg
     4741 gcttttttat ttttctttga aaggtggtga aaacatgtga gcttatttga tatctttaag
     4801 cagtcaataa gaaatgaaga gccttctgag tggataccgg attttgtata tggagaggaa
     4861 gtatctgctc gggcatacct caaaattatg gcaaaaaata ctgttttaga ttttgttgca
     4921 agaactatgt caacgttaga agttaagttt aaaaataaag atgggacatc agactgggat
     4981 tatattctaa atgttcgacc taattctgat atgtcagcag ctacattttg gcaaaaattt
     5041 ttttatcggc tcttagatga caacgaagtg ttagtaatat ttacagatga taatcaattg
     5101 ctgattgctg atgattttag tcgaacggag tatgccgtgt atgatgatgt ttttacaaat
     5161 gtgatggtaa aaaattttgt ctttgaacgt tcatggaaca tgtctgacgt catttatatg
     5221 gaatataata acgaagaatt agaaaggttt acacgaggtt tatttgaaga ttacgccgcg
     5281 ttgttcggac ggattctaga agttgcaatg cgtaacaacc aaattcgtgg gtctgtatcc
     5341 attgattcaa cgggaacggc gaatgatgaa aaaggaaaag atggtaagac tagatctcaa
     5401 agattacaag aatatattga taggatttgg aactctttta aaactaactc agttgcaatt
     5461 gttcccaaag tgaaaggctt tgagtatgag gaatacacca ataaacaagg cgtatcgaat
     5521 caatcgctag atgaactgaa taagatgaag tcgtctttaa ttgatgatgt tgccaacgcc
     5581 ataggagtac ctacggcgct tatttatggt gaaaaagctg aactggattc aaatattaaa
     5641 gcttttcgta aattgtgtat tgctccacta gttaaaaaac ttcaagacga attggtggca
     5701 aaaataatta gtcgtcaaga gtacaaaaac ggggaacgta tagaagttaa aaaagttctc
     5761 caaaaagatc tattagagta tgctactcaa attgataaaa taacatctgg tggtgcgttt
     5821 tatattgacg aagttcgtga tgaagttgat tatgatgagt tgccaaataa tgaaggaaaa
     5881 attcgtattc gtacgaaaaa ctatgaagaa actgtgaagg gaggtgagaa cgacaatgcc
     5941 gaaagctaaa aaagtaccat ttcagtttac taacgaaatt cagaatggta agcacattct
     6001 caccttgagt ggaaatgtac agaaaaaata ttggcgtgat gatgatgtca taaacgctaa
     6061 agatattcgt gactctctag atactgtaac cgatgatatt gtaatcaagt tgaacagccc
     6121 aggcggtgat gtgtttgaag ggattgagat ttacaattat ttaaaagatc acccatcaaa
     6181 tattactgta gaagttacag ggcttgccgc aagcgcagca acgtttattg tcgctggtgc
     6241 caatgatgtg attatgaatg ttgggacttc tttgatgatt catgaagctt ctacatttgc
     6301 ttgggggaat aaacaggaca tccaaaaaac gttaaacgca ttggaaacga tcgatgattc
     6361 tatcttagct atttattcag ataagactgg ccaatctgtt gataagttgc gtgaatggat
     6421 gaatgaagaa aaatggttta cagcagatga agctgtcaaa tatggatttg ccaattctgt
     6481 gaagcgtgaa gaaccacaaa atgaacctca aaatattgca gcgatgattc aagatgctgt
     6541 tgccgttgca atagcgaatt taaaggaacc tgttactaat aaaatcgaac aagaatcaaa
     6601 acaaaaatca ttaatcgcac gattgcgaaa aggagaataa agatatgtta aaaattacag
     6661 ataaaactgc agatgcaaaa aaggcattta atgctatttc ttcgaaagaa gatgcaacac
     6721 ccgaacaaat taatgctgct ctagaagaat atgtaaccgc cattgcagaa gatgccggca
     6781 aacaggtgcg tgctgaatat gaagaattga aaaatgtgac ggacaaccgt gtattagagg
     6841 ctcgtggaat tcctacacta acagcagaag aaacaaagtt ttataatgaa gccgtaaaat
     6901 ctggaggttt cgattctgac gtagtttggc cagaaacgat tttggaacgt gtttttgaaa
     6961 atcttcaaaa cgatcatcct attttaggcc tcattaactt tacaccaact gtaggtcgtg
     7021 tcaaagtaat tagagcacgt cgtaaaggtg ttgctgtttt tggaccattg cataaagacc
     7081 ttgaaggcca actagatgct gaatttggtg caactgaatt tgttcaactt gcgttgaccg
     7141 catttttctt aatttctaat gataccttag atttaggacc acgatggatt gaccgtttta
     7201 tcaatctttc tttaagtgaa gcagtacgtg atgtttgggc taaaaaaatt attacaggtt
     7261 ctggtaaaga tgagccaatt ggattgctta aagatctaga tggtgcggtc acaagtggtg
     7321 tatatccaga taaagctagc gcagggacat taacttttgc gaaagagaag attgttcttg
     7381 aacttgcagg ggtgatgaaa aaattatcta aatacacata caaagtagat ggtgacgatg
     7441 aaggtgaagt gaaataccgt tctgttgctg gacgagtgta tctaattgtt aatcctgttg
     7501 attactggga tatcgtagct agagttacat ttgcaaattt aaacaatgtg tatgggacta
     7561 cgatgccatt tatcgctgtt gatcatatca tcgaatctgt agacgtacca gaaagtaaat
     7621 gtattgcata cgttgataaa gagtatgaag ctactcaatc aagagctgaa aaagtctatg
     7681 tatataaaga aacttttgca atgaaacgcg caactttgta tgctgtcgac atgcttggta
     7741 atggctatcc aacaaataat gatgcagcac aagtatatga cttggacttt acagcggggg
     7801 agtaatgtcc cctgcaattg gtgagataac accgacgact gatggggcag tcattaatct
     7861 ttcgtaaatg gggttagagt atgaacactg aagaatttat aaaagaatat aaggcccgat
     7921 ttcgtatttt ccatacttcc gaagatgagg atatcgggaa acagttagag agcgggtttg
     7981 ccgatataaa atctctaatc ggcagttttg atccttccac ttatgagaaa ggaaaagaat
     8041 tagtttatga acgtactcgg tatctacgga acgaatcact agagtacttt tacgacaatt
     8101 ttcaaacaat gattatggat gcatcaattg atctggcagg tgctggatat gccaattaaa
     8161 tcaaattata agaagcctaa aattgtagct ggtgatctaa atacgccagt tacttttttt
     8221 gagtttcaac ctaataaggg tccagaacct ggtgatgaag aaaaaaagga actgtatttt
     8281 tgtacagcac tagtttacaa cccatcaatg aaagatcgtg acattcttgg cacaaaagaa
     8341 ggcgttacga taaaaatacg ggatccccat caatcttata ttcctactaa caaacataaa
     8401 gtaaggatat tagattatcg atatggagat aaagtttggg aaataatcga tgtctcaccg
     8461 gatttggaag aaaattcaat tgtaaagata gtattagaat taattactta aatttggtga
     8521 taaatgatga gtaacgtaaa gtttgaaggc gttcaagaaa caatccaagc tttagaaaaa
     8581 aagtatggag aaaagaaaac taagacatta acaaaaaaag caatcaatgt tggcgctgaa
     8641 aaagtagaaa aacagttgca aacggacatg accgttttta aagacaaagg ttatacgatc
     8701 gctgaggttg ttcgcaagaa cgctacgtat agagattaca aggcagaagc tgaaatcggt
     8761 tggaatggtc cacatcaacg atatagaatc attcatttga atgaatgggg atatacacga
     8821 aagggcaaac aaattagacc gagaggattt ggtgtaataa caaaatctct taagaattcc
     8881 gagctgttgt actttgatgc tgtaggagag gaggttaaaa agagtttatg aaagatatgc
     8941 ttaatatcat ttacgaggct ctttgtttga atgagtatat tcattcaatg acctacaacg
     9001 agaataccga gcaatatcgt attaaatttt atgagcaacc tgaaaccgcc gataaaacag
     9061 gtccaatgat cacaattcgt cctgtagata cacctaacga agcgtaccac ggaagtaaca
     9121 aagagctttc tgtggagcat atgatacaga ttgacgttga atcgaaatat agagcaacat
     9181 gcaagcagat tcaatatgag ataaaaaaag agatgaaaaa actaggcttt ggccaaattt
     9241 ccggtcaagg gttagatgaa tactttactg aaacaaaccg ctttgtagac gcgcgtcgtt
     9301 acgaaggaaa tacaaaaatt tacgataccg aatattagaa attaagacac gaaaataacg
     9361 tgtctttttt tattacccaa aattaggagg aaaattttat gactttagta ggattcaaaa
     9421 aaatgacaat cggggtcttt gacagcacag ggaagattcc agcagcaaat ttatatgtaa
     9481 ttgaaggtga acaaaacaaa ggggcgactg tatctgctga aatcagtggg ctatcaaaag
     9541 aaccatcaaa agtttacgga tcgaacatcg cttactatgt ctctcaaaaa ggaacaggcg
     9601 atgtttcagc tacttttgga ttgttagatt taccaacaga agtaaatgac aaaattcttg
     9661 gttacaaaac cgatacaaac aaaatcagct tcttgggaga agatactgag ccaccatatt
     9721 gtgcaatttt gatggaatcg gaggatttga acggcgatac agcaatgctt gcgatgttca
     9781 aaggaaaatt cagtcgtgaa tctattaatc ttaacactac gacaaatgaa gctttcgaac
     9841 cagaagcaga agaatatgta ttttcagcga ttgccaatga tgttgaaggt gatgctaaag
     9901 gccaaacagt cgcaaaatat gttggagacg aagaagcttc tattactggt ttgaaagaga
     9961 tggtatttcc tgcagcgggg gagtaactag ccccgttgtt ggagcaatca ctcccactat
    10021 taatggggca acagttgaat taagttagga ggataactat gccagataca tttaaaatct
    10081 ataaaaaaaa tggtactgaa tttacaaaag tcgcagaggg tgaaagtcca ctttctatta
    10141 ctggaattgc agcaaatact caagttgcta aaggagatta tcaagctacg cgtgtggtcg
    10201 gaggtcaaga atcagtaaaa gtagatattc ctgcattcac cactcttcca atttcagtta
    10261 ctggtgtgac gttggataaa acgacagctg aagttgaaca aggcggaacc ttaaaattaa
    10321 ctcctactgt aacaccagca aatgcaacag acaaaacagg aagctggagc agttcgaata
    10381 cagctgtagc tacggttact ggaggcaacg ttacagtaaa aacagacgct gaagttggcg
    10441 gaactacaga agtttcattt actactacgg atggcggtaa agtagcaaaa tgtactatta
    10501 cagttattga aaaagcagcg ggctagtctt cggactagct tttttatttg gaaaataatg
    10561 gaggaatcta aatggcaaaa gtaagaattg aattgaaaga agaatcagga aagaaagtct
    10621 atgaaaacat agacacaaca gggaaagatt atcgaaaagc tttagaaaca atcaaaaagc
    10681 taaatgaaga cggagtaatg atttggaatc gtttagacat ttatttggat tttgctgtag
    10741 gtatcttcag ggatagcaaa ttgacagctg aacaaattct tgatggctta ccatctgaga
    10801 aggtaatgag cacattggac gacatcctag gagaagtaat ggggattgaa agtaatccag
    10861 acccagacgc aaaaaagtaa cacccgaaga agcagaagaa atgtacttaa acctatgcag
    10921 agagttagtg aaagcgggat ggtcactatc tgacatagaa gataattcct ttgaaatgat
    10981 gatgaaaatt gcgtgcactt cgcctaagaa agagaaacaa aaagaagttg atcttaaaga
    11041 cttcttgaaa tccatgtaga gaggaggtaa acatatggca aatggaaaac ctttaggcaa
    11101 tatgattatc aaactagact tagacagttc agctttttca aaaggtctag ccggagctaa
    11161 aaacgctgtt aaccatcaaa tgaaagcaat gaaatcacag atgcaagtaa tgaactcttc
    11221 aggaaattct ctcggtgctt tacaagcaaa atacaatggt ttaggcggag ttctacaagc
    11281 aaatgaaaag caagtagaat tgctcactaa ggcttataag gatagttttg actctaacgg
    11341 aaacgctact gcttcgactg ctaaatacgc taatcaattg aatcaagcaa cagctagatc
    11401 agcaagttac gaatctcaga tgaaaacaac tgttggacag attgctcgga tgaaagtgga
    11461 aacggaaggg tttacgggga aactaaaggc taattctgat gtattgatta attctgggaa
    11521 aaaaattgaa gcctttggta aaggagtttc tgggatagga tctgccctaa ctgttggagt
    11581 aacagctcca atagtggcag gcgcaactgc tgttactagt gcagctattt catgggaaag
    11641 tgcttttgca ggtgtaaaaa agacaaatga tgaagttgta gactcgaatg gaaacgtagt
    11701 ttattcctat agcgacttag agaaaggtct gcgtaatctt gccacccaat tacctggtag
    11761 ccatcaagaa atagcaaatg tagccgaagc agccgggcaa ttaggtataa agactgaaaa
    11821 cgtagttagc ttcactaaaa cgatgataga catgggtgaa tcgactaata tgtcggctga
    11881 aactgcggca acttctttag cacgatttgc taatattacc caaatgtctc aaaaagactt
    11941 tgataaatta ggatcagtca ttgttgatct aggtaacaat tttgctacaa ccgaatcaga
    12001 aatcactgaa atgggattgc ggttagcggg agctggtaaa cagattggaa tgagccaagg
    12061 cgaaattcta ggatttgcta ctgctctatc ttctgttggt gtcgaagctg aagcgggtgg
    12121 gtctgccttt tcgaaggtca tgatccagat gcaactagct gtagaaaaag gatttggcgc
    12181 ttttgatcag ctgaaacaga tggcagaaga acaaggtgtt ccgtgggtta atttagtaaa
    12241 tgctgtgaga gacggtggaa aatcgttaaa agcagtatca gagcaaatgg gattcacttc
    12301 atctgattta aagaaaatgt ataaagaagc ggacaattcg aaaagttcgc ttgaaaattt
    12361 tgcgaatgtg gcaggtgtta caagtgaaca attccagaag ttgttcaaga gtgatccttc
    12421 agaagcaatc atcaagttca ttcaagggct gaaagattca gaaaaacatg gtacttcagc
    12481 aatcaaagtt ctggatgaca tggatataaa agaagttcga ctacgcgaca gtttattgag
    12541 agctgcaaat gctagtgaca tattcaaagg agcaattgaa aaaggaaacg gcgcgtggaa
    12601 acaaaataca gctttaacaa atgaagctaa taaaagatac gaaacaactg aatcaaagct
    12661 aaagatattg aaaaacgaag tgattgatac agcaatcgat cttggaggtc catttgtaga
    12721 cgcgttgcgt gatggtctaa atgtatcgaa accattgata aaaaacttag ggaatatggc
    12781 gaaagctttc tcagctatga gtgatgatca acaaagaaac attatcaaat gggttggatt
    12841 agcagcagca gctggtccag cgctgaagct tttaggaact ggtacttcag taataggtaa
    12901 agttacttca actactggaa gattgacgaa aagccttgtt gatttagcag cgaaagcagc
    12961 agaaaagaag gctatggatg cctttgcatc gtcggtaaca acagctggtg ctgcagctgc
    13021 aaagacagca ggagctggag gtattggcgg gttagtccct atgatgggaa aaacagctag
    13081 tgctgcaagt actgctgcgg gcgccagcgg aataggcgca atgacaacgt cactaggttt
    13141 gttaggtcct gctctacttg gtattgttgg cgttggtggc gcactggcag ttggctatgg
    13201 agcatggaaa ttatttggtg aagaagcttg gaattcctcg caaagagtaa agcaatgggg
    13261 aacagatgtc ggtgaggaga tagatagtac tcttgacgga gtacagaaga atattgaagg
    13321 tgcaaatggt caattcggtc ttctcaaaga gggttttact gttgatgatg caaataatat
    13381 ggcggctaat ttcgcaaccg ctggaacatc tttggaaaca tctttaacgg gtagaataaa
    13441 ggcattagac gatgcactaa aagggctacc tcaatcagtt caggatgcca tgaaagaagt
    13501 agctgaatca gaagaagaaa ccttagggca atctctttct aaaataaaag acaacaatga
    13561 agaaataaaa aaaatcagag aaagagccgc aaatcagaac agagacttga ctgtttcaga
    13621 actacaacag atacaggatc taatgaaagg aacttctcaa gcgtatgtgg atacgctcga
    13681 tgtttctgct aaagacagaa aaaagatact atcagccatg aatggtgatg ttgcacaagc
    13741 tacagaagaa gaagcgaaga cgtggttaca atctttagcg aaacaaaaag ctgcaactac
    13801 cgaaaattat agacaaatgc ttgaagaaaa aaaggcggct ttaaaggaaa agggagtaag
    13861 caaagaattc attgatgcat tagaagatga aatgaatgat tacatcaaaa ctacaaatca
    13921 aggattcgat agccaaatag ctgctataac aaataaatat ccacaattga ttaacgaagt
    13981 gagtttatca aatggtcaat tatttagcaa aacggcagct gctatggaca atactggtca
    14041 gtatacaaaa gtttttctcg aaagtaatga aaagatttta gcccaagcga agaaaacatc
    14101 ggatgaaata gcagataatg ccgaaaaaaa tgcagaacaa cttggtatgg tagctgatga
    14161 ggctacgcat gctggatcaa cttggaatgg cttagtttta gacgaaaaaa caggaaaagt
    14221 aaaaactaat gtacaagaag tagtgaatga agcaacgaag gatttcacta aatggaatga
    14281 attgaaacct gttgttcatg atgcgaaact aacaacgaat gcaaaagatg tcatttccat
    14341 agcggcagta caaaatggct attgggatga tatggaatgg aaagaaaaag aagcattatt
    14401 agaagatgag tgttctaaaa atgtatacaa ggctttagaa aatgctggta aatggaatga
    14461 gttagatatt ccttctaaag aagcaatcct aacttcaaat actcctgaag ttatgggtga
    14521 aactctcatg aatttgggat tatgggatac ttacaaactt caagtgaaag atttagattt
    14581 gaatgcgtat aaagtatacg atgctatcga tggatcagaa gaaaaattaa atagttgggc
    14641 tcaattagat aaccccacaa aagaattgtt attagataac tcagattttt ctacaaaact
    14701 ttttaattcc gaagaaatgt ttaaacgttg gaatcagtta tcatctaatg caaagtttct
    14761 ttcggctaat aatactaaat ttacagaggt tcttttatca tctgacagaa tgtggaatca
    14821 atgggtaaac cttcctgata atgagaaaag attacttgca gataacgaag atttagctac
    14881 cactgtttta tcttcagaag aaatatacgg aagatggact caattaccag ataatcaaaa
    14941 atatatgtta gctgacaata ctgacttagc aaacaaaata ttcgcttcag aagaatcatg
    15001 gaatgcatgg aaagcaattc cagacacagt taaaaacttg cttggtaata atattgattt
    15061 actggctaaa gtgaaagacg ggactgtaag tgtagaagaa tataacaaag ttatgccact
    15121 tcttaaaact ctatatggag ataacgttaa tttaaaagag gcaatcagtg gggctaaaag
    15181 ctctatagag aattacaaca aaaatatttt tcctagtgat aaaactgcag taggacacga
    15241 tgaggcttca caagctgcta aagatgcatt tgaagcgttc aacatcttcc aaacaaaaat
    15301 accagataaa attactaaaa ctgtatcagc tgactttata ggtcctatgc cgaaaaatgc
    15361 gaaaggaaca aacttccatc ctggcggagc agctatggta aacgatcaaa aagggcctac
    15421 ttatgaagag cttataacct tgccaagcgg agaaactttt atacctaaag gacgtaatgt
    15481 cgttttagac ttaccaagag gttcgaaagt actgaacgcc actaaaacta aacgtctagt
    15541 gcctaaatat gctgatggta taggaaacat aacgacggtt tcatctacgc taaacttaga
    15601 tgctttaatt ttagctatta acgagctaac gacggtattg agaactcaac tgatggcagg
    15661 aacgactgat gctaaagata ctaaactatc agatgaaaca aaagttgccg atccgattat
    15721 tccggacagt ctttctgaaa aatcagatca gtatctagga attggagctc aatggttaac
    15781 taatctaatg aatggttgga actcagcggt tcctcaatat atgaatagtg aaatggtttt
    15841 tattacaaac tatctcaatg cgctaaaact tcaaaacaat cctaattata cgcaaggagc
    15901 aacatggaat aagaacttac tgaatggatg gaatagttta actgggacct ttattgctac
    15961 aatcaattca ttttgtaatc aagcaatggt cacgcttaga aactacaaca ctcctatgta
    16021 caacaatggc cgaacgtggc agcaaaataa tcttaatggt tggaattcat tgtatggatc
    16081 attcatagcc cgtgtaaatc agcttggtaa cgattcgatt aacaaccttc gttctaaaag
    16141 tggtggtttt tacaatgcag ggacattctt gttgcaatct ttaataaacg gaatgaattc
    16201 gttaggtaat tctctttcta ctacaatgaa caatgtcgct aatacaatgg ttggtggtat
    16261 aggcaaagga gttaatggcg tcatatcggg agttaactat gtgcttaagg aagtcgaatc
    16321 aagtaagaat atcggaaact ggccaattcc acaatacgct aagggaactg atggacatcc
    16381 tggtggactt gctatgatta atgatcaaaa aggcctagtt cacgaagaat atgttcagat
    16441 gccagatgga cgtagtttta ttgccaaagg tagagatctt cttgttaatt tgcctaaagg
    16501 tgctcaggtc ttaaatgctt ctttaacaaa gaaattaaag gaacgtttaa atattccaca
    16561 ctacgaaaaa ggtattggca atttagatat tgttgatttg ttagatgatg aaaaaagaat
    16621 gttagaattc ttaactagca aagttgattt ttcaggcatt aatgaaccat ggcttgatat
    16681 gactaaatca ggaacgtcat taatgtccaa agctgcaaat acaatgcttc aatcaaaatt
    16741 aagtgaattc ttcactcatg gcaattttga tggtgcagta aatgccaatg gtgtttatca
    16801 atatttagtt gatgtggcac agaaagtaat gggtaagttc cccggactta cagtaacttc
    16861 tggatatcga gcaggagatg cctactatca cggaaaacgg caagctatcg acttggctta
    16921 tccaggtatt tctggagatc cgagatatac agcagcagca aattacgctt ttgaaaaatt
    16981 cccttcaaaa attgcgtatg tcattacgaa tggacgtgta cgtgaccgca tgggattatc
    17041 tggaacaagg tcaagcggac aatggacgaa ttggccagat ggcgatcact tcgatcatat
    17101 tcatttaaac ggttcgatgg ggtctggcga tatattcaat ggaggagttg gaggaagtgg
    17161 tgttgcacgc tggcgttctt atgtgtctaa agcattgaaa atgaatggat taccagctac
    17221 agcagcgtat ataaatgcct ggatgtccca aatccaaacg gaatctggtg gtaatgagaa
    17281 agcaatcggc ggcaatgatg ggctcgccga aggcaatgca acaggattac ttcaaacgaa
    17341 acccggaact tttgcagcta acgcttttcc tggtcacgga aatattatga acggatttga
    17401 taacatgtta gcagcaatca attatgctaa gaaacgttac ggagttgcag gtatgttgca
    17461 agttatcggg aaaggacatg gatatgccaa tggcggcttg attaccaaag acggactata
    17521 tcgagcagga gaaggaaata aaccagaaat ggttattcca ttgacaagaa aaacaagagc
    17581 tatcgaactt atgggtcaag cattagcctt cctttctgga agcaataaaa aagaaacgaa
    17641 aagtgagatt aattctgtag ataatagaca tatcctacaa gcgatagaaa aacagtctcg
    17701 tatgacaaat gatctgctat cattattact ctcgtttttt aaaagtaaca atcgatcaga
    17761 taaagaacta gctttagata ttcagaaaat tttaatcagg aggacatagt atttgaggac
    17821 agtaatatta agaaataaat tgaatgaaga aattgatttg tccacagaag attattttgc
    17881 tactgaaatg tcgaatctag gttttgaagt tacaaaagag catattggac aatgggggag
    17941 tttcagagaa ataggagaga gtattgaatt gtctgaattt caatcatcag ttatgatttc
    18001 tgttcacgga tttagagaac aacaattata tggttcatta gttgaatttc tggctaatgg
    18061 accttttgaa ctagaatttt cttttgattc agaaacaatg atcaggaaat gtagtttgaa
    18121 atcattatct aaaacagaaa ttgattccca aagctcattg ctaacagata ctttgaatct
    18181 atattttact tcaagctggt attctattaa gcgtgaaaaa ttaatacaga attcaacttc
    18241 tctcaataca cgtggaaaag tttactcatt tgttcgacca tatgtctaca ccaaaaatgt
    18301 atgggaaaag aaaggtgtat ttaagttcag taataattct gtatatttga caaatagtac
    18361 agagcgaatg tctccgttaa aaataaggat tattggagag tgctcaaatc catattggga
    18421 agttatccaa aactctaaag tagtagcttc agatggctat tttattaata tgaaagacac
    18481 acaaatatta gaagtgtcta gtttgctaga agaccaaacg gcaattttaa aagatgtagg
    18541 tggagttgaa tcttctgttt atcaacaaca agattttact aaatcaaact tcgtccaagc
    18601 gcctctagga gaattcagcg ttgtttttca tgttggacat gcaaatgtag agattgaact
    18661 ttatgaggag cgtgatctat tttgatttta gccattaccc tctttcaccg agatatgaat
    18721 ttatataacg aacgtttaat ttcgactgag ttcgaatttg gaacggatga aattggagaa
    18781 gaagtaagta attttattac agataaatat gtccatgtaa aatcaggaga ttttctacta
    18841 gcaaaattta ttcctagcgg gaagattgct tattttggag tgattagctc gcaagaagac
    18901 aaaaaaataa attgcaaagg attactcagt ttggccgata gcgagatacc gactgtaaaa
    18961 atatcaggtg ctaattatga agagcatatt cgacgactga ttgaatacta cttattgaac
    19021 gatccaacaa aacaactaaa aaatattttg gaggtaaaaa cagaaagcgt tacttctcat
    19081 tcttatcaag ctgttgacac aaataagaga cgattgaatg catatataat aaatgggttc
    19141 aaaaaatata atgttaaatg gtatgtcaaa gagattaaag gtaaaaaaat atatactggt
    19201 attcgagcag ttgatgattc aatttatatt aaagataatt catctgagtt taatgactgg
    19261 gatgtttttg tgcaatctcc tggaccaggg aacgagaaca aattattgat cgtagataaa
    19321 gcaatgaaag atatagaaaa tcccttggta ttgtctacgt ggtatttaga tgacgaaaat
    19381 aatcttacgc aagattcaac gaataagaac attgcattgc ctaccgttaa tttagtaaat
    19441 atatatgatc aaactcaaga agatagacct tcttatgaag aggtagctaa atcggagctt
    19501 aaaggcaacg cctattcaca tgaaattaaa gttaatgttg tgctaaatgc tagaaatatc
    19561 aatgtagaaa atatcgaaac aggaatgtta gcgactattg tttacaaaga gcagacttac
    19621 aaatctgttc taactgcctg gcgaatatcc agcagtaaaa agaatataga attgacattt
    19681 ggtaatgtcc gaagtcgttt tatggattat tttgaagata atgggggata aaaaatggtt
    19741 agcaatgtgg atggatatca atttgaaaac gtgaaagtaa gcgcagaaaa tgatgctaga
    19801 ctttatcacg ttttatataa tcggaaaaat caggttattg atggttacga tcagtctatg
    19861 aatttatctt caagcggatt aacagtaaaa gttgctgcag gagcagcgat tattcaaggt
    19921 cgtatggtcg ttgttcgaca agaagaaagt ataacagttc cagcaaactc aagtggttat
    19981 atagcattaa cagtggattt gacacaagaa gttatacctg gatctattct tccagaatcg
    20041 gaagaatatg aatggactaa taatcaagtc aagctagaat ttataacaaa agttataaaa
    20101 ggtaatttaa acaatggtga taaggtctat aatttaccac tatgctcagt taattctact
    20161 ggatcaactg tttcaatctc aaagatatcg gatagttacg agctgactct ctctaaagga
    20221 gaaattttgt ggagggggac tgcgttaatg catgatactc aaactgtcca accttcaaaa
    20281 aaaatttggc agacagtttc aggttttttg ttagtatggc tcccatacga aaacgggcaa
    20341 gcaattgagg atagatatgt aactacgcct ttttataagg agcgtataac ttttactaat
    20401 gttttaggag aaattgtttc aggatttgat gactatcaca aaaagtggtt tagtaaacga
    20461 ataaactata attcgaatac taatatattt acaggtgcag caagcaatgc aagtggagat
    20521 aacgcaaata tggtgcttag gtatatagtt tctttttagt taggaggtat ggaagagtgg
    20581 caaatttaga aattaaatta tctgcaaata agagacagcc ttatctacgt caccgtgttg
    20641 ttggtagagt tggtgatggg gggctcacaa caatcaatgt acaacttctt gaggaagatg
    20701 aaattacacc ttttgtaatt aatctaaacg gtactttgaa atttgtgggc gaagtttcaa
    20761 acggtaacta taccgaggga gaaccagaaa taatcgattc gactaatggg ttaattagtt
    20821 acacgttcac taagtcaaat ttcagcacga gtcatcaatt caaacaagca tattttgaat
    20881 atgtagatcc taacggcaaa aaagtaactt ttcagaactt catcatagac gtgttagaac
    20941 gagtagatat taattcggag caagcgaaat actatatttc ttcattggaa aaattacaga
    21001 gtgaaatgca aaccactttc aatcagttca ttagtgataa acaggtccaa tacgatcaaa
    21061 tctactcgaa atataacgaa ttagtaagat tgataaatga atcagataag caagtaaatg
    21121 atcgtattga taaggcaaat aatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg
    21181 gcaagctgaa aaagatgtac agtaacagca ttga
//
LOCUS       DAPCMU010000039        20935 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPCMU010000039 DAPCMU010000000
VERSION     DAPCMU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.039"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            591..1280
                     /locus_tag="RD744_001175"
     CDS             591..1280
                     /locus_tag="RD744_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557188.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="RD744_001176"
     CDS             1267..2475
                     /locus_tag="RD744_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0557189.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="RD744_001177"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="RD744_001177"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDU0557190.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="RD744_001178"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="RD744_001178"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDU0557191.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="RD744_001179"
     CDS             complement(6578..7273)
                     /locus_tag="RD744_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDU0557192.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="RD744_001180"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="RD744_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDU0557193.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="RD744_001181"
     CDS             complement(8472..8663)
                     /locus_tag="RD744_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDU0557194.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="RD744_001182"
     CDS             complement(8684..9574)
                     /locus_tag="RD744_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDU0557195.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="RD744_001183"
     CDS             complement(9561..10328)
                     /locus_tag="RD744_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDU0557196.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="RD744_001184"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="RD744_001184"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDU0557197.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="RD744_001185"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="RD744_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDU0557198.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="RD744_001186"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="RD744_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDU0557199.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="RD744_001187"
     CDS             14899..15282
                     /locus_tag="RD744_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557200.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="RD744_001188"
     CDS             complement(15450..16208)
                     /locus_tag="RD744_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDU0557201.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="RD744_001189"
     CDS             complement(16226..17044)
                     /locus_tag="RD744_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDU0557202.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="RD744_001190"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="RD744_001190"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDU0557203.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="RD744_001191"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="RD744_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDU0557204.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="RD744_001192"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="RD744_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDU0557205.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="RD744_001193"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="RD744_001193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDU0557206.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5987 a         4261 c         3836 g         6851 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttat
//
LOCUS       DAPCMU010000040        19781 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPCMU010000040 DAPCMU010000000
VERSION     DAPCMU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.041"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            202..1077
                     /locus_tag="RD744_001194"
     CDS             202..1077
                     /locus_tag="RD744_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557207.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="RD744_001195"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="RD744_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="RD744_001196"
     CDS             complement(1642..2973)
                     /locus_tag="RD744_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557208.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="RD744_001197"
     CDS             complement(3122..3835)
                     /locus_tag="RD744_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0557209.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="RD744_001198"
     CDS             4010..5434
                     /locus_tag="RD744_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0557210.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="RD744_001199"
     CDS             5733..6440
                     /locus_tag="RD744_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDU0557211.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="RD744_001200"
     CDS             6427..6750
                     /locus_tag="RD744_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDU0557212.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="RD744_001201"
     CDS             complement(6839..7876)
                     /locus_tag="RD744_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0557213.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="RD744_001202"
     CDS             complement(8197..9180)
                     /locus_tag="RD744_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDU0557214.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="RD744_001203"
     CDS             complement(9595..10365)
                     /locus_tag="RD744_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557215.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="RD744_001204"
     CDS             complement(10358..11293)
                     /locus_tag="RD744_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557216.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="RD744_001205"
     CDS             complement(11609..13258)
                     /locus_tag="RD744_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDU0557217.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="RD744_001206"
     CDS             complement(13375..15978)
                     /locus_tag="RD744_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDU0557218.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="RD744_001207"
     CDS             complement(15990..17039)
                     /locus_tag="RD744_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDU0557219.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="RD744_001208"
     CDS             complement(17065..17379)
                     /locus_tag="RD744_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDU0557220.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="RD744_001209"
     CDS             complement(17395..17841)
                     /locus_tag="RD744_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0557221.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="RD744_001210"
     CDS             complement(17842..19764)
                     /locus_tag="RD744_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDU0557222.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         6044 a         3955 c         3219 g         6563 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga a
//
LOCUS       DAPCMU010000041        19493 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPCMU010000041 DAPCMU010000000
VERSION     DAPCMU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.042"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            133..3660
                     /locus_tag="RD744_001211"
     CDS             133..3660
                     /locus_tag="RD744_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDU0557223.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="RD744_001212"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="RD744_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDU0557224.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="RD744_001213"
     CDS             7448..7789
                     /locus_tag="RD744_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0557225.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="RD744_001214"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="RD744_001214"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDU0557226.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="RD744_001215"
     CDS             9146..11566
                     /locus_tag="RD744_001215"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDU0557227.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD744_001216"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="RD744_001216"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDU0557228.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="RD744_001217"
     CDS             13000..13737
                     /locus_tag="RD744_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0557229.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="RD744_001218"
     CDS             13751..14566
                     /locus_tag="RD744_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDU0557230.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="RD744_001219"
     CDS             14579..15226
                     /locus_tag="RD744_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0557231.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="RD744_001220"
     CDS             15242..15898
                     /locus_tag="RD744_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0557232.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="RD744_001221"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="RD744_001221"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDU0557233.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="RD744_001222"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="RD744_001222"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDU0557234.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="RD744_001223"
     CDS             18240..18758
                     /locus_tag="RD744_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDU0557235.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="RD744_001224"
     CDS             18865..19365
                     /locus_tag="RD744_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0557236.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6559 a         3346 c         4184 g         5404 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaa
//
LOCUS       DAPCMU010000042        19233 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPCMU010000042 DAPCMU010000000
VERSION     DAPCMU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.043"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(255..622)
                     /locus_tag="RD744_001225"
                     /pseudo
     CDS             complement(255..622)
                     /locus_tag="RD744_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
     gene            772..1254
                     /locus_tag="RD744_001226"
     CDS             772..1254
                     /locus_tag="RD744_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDU0557237.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1291..2301
                     /locus_tag="RD744_001227"
     CDS             1291..2301
                     /locus_tag="RD744_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDU0557238.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2416..3129)
                     /locus_tag="RD744_001228"
     CDS             complement(2416..3129)
                     /locus_tag="RD744_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557239.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3319..3909)
                     /locus_tag="RD744_001229"
     CDS             complement(3319..3909)
                     /locus_tag="RD744_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDU0557240.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4393..4620
                     /locus_tag="RD744_001230"
     CDS             4393..4620
                     /locus_tag="RD744_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDU0557241.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4636..5802
                     /locus_tag="RD744_001231"
     CDS             4636..5802
                     /locus_tag="RD744_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDU0557242.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6036..7079
                     /gene="hrcA"
                     /locus_tag="RD744_001232"
     CDS             6036..7079
                     /gene="hrcA"
                     /locus_tag="RD744_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDU0557243.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7173..7736
                     /gene="grpE"
                     /locus_tag="RD744_001233"
     CDS             7173..7736
                     /gene="grpE"
                     /locus_tag="RD744_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDU0557244.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7787..9616
                     /gene="dnaK"
                     /locus_tag="RD744_001234"
     CDS             7787..9616
                     /gene="dnaK"
                     /locus_tag="RD744_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDU0557245.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9767..10933
                     /gene="dnaJ"
                     /locus_tag="RD744_001235"
     CDS             9767..10933
                     /gene="dnaJ"
                     /locus_tag="RD744_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDU0557246.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11015..11680)
                     /locus_tag="RD744_001236"
     CDS             complement(11015..11680)
                     /locus_tag="RD744_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557247.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            11656..11829
                     /locus_tag="RD744_001237"
     CDS             11656..11829
                     /locus_tag="RD744_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557248.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11874..12611)
                     /locus_tag="RD744_001238"
     CDS             complement(11874..12611)
                     /locus_tag="RD744_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0557249.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12613..14115)
                     /locus_tag="RD744_001239"
     CDS             complement(12613..14115)
                     /locus_tag="RD744_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDU0557250.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14374..16368
                     /gene="uvrB"
                     /locus_tag="RD744_001240"
     CDS             14374..16368
                     /gene="uvrB"
                     /locus_tag="RD744_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDU0557251.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            16378..19194
                     /gene="uvrA"
                     /locus_tag="RD744_001241"
     CDS             16378..19194
                     /gene="uvrA"
                     /locus_tag="RD744_001241"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDU0557252.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         6345 a         3347 c         4086 g         5455 t
ORIGIN      
        1 atattttctc gtattttttc cttattcaaa agttttgcag gaaaattgct gaaaaaataa
       61 ttttactatg tagaaaattc aaagccattc aatcagtgat ttttttcagc acaagaaaga
      121 ctccttataa aaatagcaga tactttgtca attaagtgtt taaccaggga tgataaaaag
      181 gataggattg aacaaaacct gtataaggtg atgaatagtt tcctcgccag taaaaggttt
      241 attcgtataa agctttatcg ccaaaattta tcatgacctt ttttatcttc aatattaaat
      301 gaaatcaatt gttcaatcaa ttcccagttg attacacttt tccaaggaac tttgatgacg
      361 ttatccgtat atacgtagcc tgcttcttgg atattttttt caagggcacg aatcgctgct
      421 ttttccggtg cgatagaaaa atgattttta gcaaaactaa atgcaataat aaaggtacca
      481 tgtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg
      541 ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg atttgattga
      601 tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac
      661 cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta
      721 tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac
      781 ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt
      841 tatacaaatg aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc
      901 aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg
      961 aaacaaacgg atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca
     1021 tcagaacgca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat
     1081 gaaaaacaga ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt
     1141 gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc
     1201 cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt
     1261 aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat
     1321 gcaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc
     1381 gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt
     1441 tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa
     1501 atgatgcaag atgaaaatat ccaaatgatc tttccgactt ggggaggaga attattgatt
     1561 gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca
     1621 gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga
     1681 actaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt
     1741 gtactgaaaa caagagaagg agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa
     1801 gtgtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg
     1861 caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg ttgtattgat
     1921 acgatcaggc atttaatcgg tactccttat ggagatgtcc aatcttttaa aagaaagtat
     1981 aatgatggaa agccgatctt gtggtttcta gaaaactgtg agctatcgtc agttgattta
     2041 agaagatcac ttgttcagat gaaactggca ggctggtttg atggcagctc ggggatcatg
     2101 ttcggtagaa gtgcggcaga ggatttgtta gattataaaa tgaaggatgt atacgaagat
     2161 ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca
     2221 caactgacat tgattaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact
     2281 gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtctatca aagaccaata
     2341 aatctaaata gaaaagaaag tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat
     2401 aacatattag aaagtctaat tatctataac tgtcacatcg attaactgtt tcacgtcatt
     2461 gggatttcct gtaggaatag ctactacaga tgcattatca atgatttctt gagttgtttc
     2521 tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg
     2581 ataactagtg attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta
     2641 tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat cataaggggc
     2701 gataggtgtt cctgcaggat aatgaataac atgtaattcg aaatcacccg atgtatctct
     2761 aaaagtatta cctctaatag ctagccaaac acgtgcgtac tcaatttgta gatcagaata
     2821 tcctgctaac ggatctattg actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga
     2881 agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt
     2941 actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc
     3001 agctgaactg gtttgtctga ttgaactttt gtctgtggtc gcagatggat cactattttc
     3061 tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa
     3121 ctttttcata taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt
     3181 ttgaatccgt aaaaccttgt ctatgaatat aaatagagtg tatcttcttt gataaatgtc
     3241 agaaaaagtg tgacaaataa tcatcagtaa aattacttgt cacactattg ataaggagta
     3301 tttaattgaa gagaatgatt aggagaaagg atctccgtac tttttatagt aatcatagaa
     3361 tccaagtttg tatagacgaa tcgttttttc tcttatttct tctgtttcaa aatggactgc
     3421 ttgaagaatc aagtctgtga attctatagg agctgttcca ttcgcagtaa tcaaattacg
     3481 atctttcaca gcttgttgtt ctataaaatc ttctttattc tgataatttt ccgattctaa
     3541 ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc tctgagctaa
     3601 ataatcaact gccccacaaa tagcagctac tggtacgtct gtttttaaca atttatcgat
     3661 tatttgatat aaatgatgat tttttatgtc ccatgaattg ccaccgatca taataaaaag
     3721 gtcaactttt tcaggtattt gttcgattaa gtaatcgatt tttgttttta atcctccaat
     3781 ggaagtgacc tctgattgga tagaagcagt tttgttttgc catttctcat tttggtttag
     3841 tgtgctagta atataagcac cttcccaatc ggcatattcc tctaatagaa acgtaatcgc
     3901 agttttcatt ttcaagctct cctttactat ctataacaag gacaacagtt ttaacctaca
     3961 gacattcatt aaaattgggt agcgacagcg aaaacaacaa ttcttaagtt ttagacagag
     4021 tatacagata gtcaacttaa ttaaatacga actaaataaa aatgacaggt aatcattttt
     4081 gtttaccagc attataacat tttgcataca tggatgacaa aagaaccaat tcccacttgc
     4141 ttgagtacaa tattaaaaga aactggaagg caaaaagtaa ttatagaaac gaaaaataaa
     4201 tcatatcggg cactcagaca tgaaaacaac cctagagata tataactagg taaccagttt
     4261 aatgaaagaa aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc
     4321 aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt
     4381 aaggaggaat ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag
     4441 gtcagctttg cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac
     4501 aataagcaag catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat
     4561 gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa
     4621 ggagaaagaa tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac
     4681 attcatatcc cattttgcga gcatatctgc tattactgtg attttaataa agttttttta
     4741 gaagggcagc cagtagatga gtatgtggaa atgctgctga atgaaatgaa aataatgcta
     4801 gaaagtcatc cgacagcgaa tttagaaaca ctttacgtag ggggcgggac accaacttct
     4861 ttatctgcta aacaacttga tcgactgtta tcaggtgcta gagagatcct gccttttcaa
     4921 aatggtaatg agtttactgt agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa
     4981 gtaatgaaaa actatggcgt caatcgttta tccatgggag tgcagacatt tgataatcgc
     5041 ttattgaaaa aaattggacg taaacacacc gcagaagatg tgtatcaaac tatgcaattt
     5101 ttagaagcag aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa
     5161 acactagaag gatatctgga tactttggaa aaagctttgg ctttggatct gcctcactat
     5221 tctttgtact cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga
     5281 agactcgaac tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca
     5341 atggaaaagc atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa
     5401 tctcaacata acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct
     5461 agcggttatc ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc
     5521 ccattacgag caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa
     5581 atagaagaag aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc
     5641 tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag
     5701 caagaagggt tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggaccttt
     5761 ttggggaaca atgtgtttga gcgctttttg tttgaaaaat agtttaaaga atatttgttt
     5821 tttatctgaa tttaaataaa aaaagttcaa aaatacaata aaggactatt gaaacgttga
     5881 tataactgga tttacatcaa ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa
     5941 atggagtctt tctcttgaca ggagaagggc ttcttgctat atttataagt ggattagcac
     6001 ttagaatata tgagtgctaa tgagaggtga ggaagatgtt aacaaagcga caaaacgaca
     6061 ttttacgtct gatcatccaa cactatacga gtagtggcgt gcccgttggt tccaaaacat
     6121 tgatgtctga gggagtcaag gccagctcag caacgattcg caatgatatg aaagcattag
     6181 aagatgaagg attgctgttg aagactcact cttcttctgg gcggattcca tctgctttag
     6241 gctatcgtta ttacgtggat catttgttaa aacctgctcg cgtagcagaa gatgatttgc
     6301 tgctgattcg tcagtctcta ggcagagaat ttcatgaaat caatgagatt attaagcaat
     6361 ctgctgagat attatctgag ctgacaagct atacgacttt ttcattggga cctgaaataa
     6421 aagaccgccg actgactggc tttcgtatcg tacccttgaa taatcgacag attattgcta
     6481 tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt cactcttccc gaatatatgg
     6541 gaagtcagga actagaaaaa atggtacaga ttgtaaacga ccgtctagtc ggagatcctc
     6601 tgatgacggt gtaccataaa cttcgtacgg agattccgat gattcttcat aaatatttcc
     6661 aaacgacaga agggatgtct cagttatttg acaccgttct tggtcatgtt tttgaagata
     6721 aggtttatgt cagtggacaa atgaatctct tggattatga gccccgtcaa gatcttgatc
     6781 agttcaaatc gatgttttct tttatgaaag actcagacgg acttacacaa atgatcactc
     6841 cgatggatag caatattcat atcaagattg gtgctgaatt aggcaatgat ttattgcaga
     6901 atatgagtat gattcaagca agttacgaga tagatggtca tggaagaggt acaatagcct
     6961 tgttagggcc tactagtatg ccttattctc ggatgttcgg tctagtcgat gtttatcgaa
     7021 gagaactagc agctaaactt gctgattact accgttcgct tgacttgtca aactcgtaat
     7081 gtgaataacg aagctatttg cagctgatcg ttctctcttt cttagtaaga gcaatcaaac
     7141 agctgactga ataatgaaag gaagactgta tcgtgaaaga aaatcaagaa gaactagata
     7201 aagaaatgac agatgctcag ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg
     7261 gtatttcaga agctgaagcg gaagaattcg aaacagcgaa attaaaagct gagttagaag
     7321 aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa
     7381 aaaatgagcg tgagcaatta caaaaatacc gttcacaaga tttagccaaa aaattacttc
     7441 catcaattga caacctggaa cgtgcattag ccacagaagt gtcagatgat caaggggcag
     7501 gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg taacgcactg gaagaagaag
     7561 gaattgaaaa aatccctgct aaaggagaag cctttgatcc taatcttcat caagcagtac
     7621 aaactgttcc agcgactgaa gatacaccag ccgatacgat tgtagaagta ctacaagaag
     7681 gctacaaatt acatgatcgc gtgttaagac caaccatggt cattgttgca caataattgt
     7741 gcggatataa aacttacact ttcaattaaa gaaggagacg ttaattatga gtaaaattat
     7801 cggtattgac ttaggtacga ctaactctgc agtagcagta ttggaaggaa acgaagcaaa
     7861 aatcatcgct aacccagaag gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg
     7921 agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc
     7981 atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta gatgtagaag gtaaatcata
     8041 tacaccacaa gaaatctcag caatgatcct tcaatatatc aaaggattcg cagaagacta
     8101 tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca gcttacttta atgatgcaca
     8161 acgtcaagca acaaaagatg ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa
     8221 tgaaccaact gcagctgctc ttgcctatgg tttagataaa acagatcgcg acgaaaaaat
     8281 cttagtattc gaccttggtg gcggtacttt tgacgtatct atccttgaac taggagacgg
     8341 tgtctttgat gtattgtcaa cagctggcga caaccatcta ggtggggacg actttgataa
     8401 caaaatcatc gactacatgg tagcagaatt caaaaaagaa aacagcattg acttgtctca
     8461 agacaaaatg gctttacaac gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc
     8521 tggcgtatca agcacacaaa tcagcttgcc atttatcaca gcaggagaat ctggaccatt
     8581 gcatttggaa atgacattga cacgtgcaaa atttgatgaa ttaacagctg acttagtaga
     8641 acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca ggtttatcac aatcagaaat
     8701 cgacgaagtt attttagtcg gtggatctac tcgtatccct gcagttgtag aagcagtacg
     8761 taaagaaaca ggaaaagaac caaacaaatc agttaaccct gatgaagtag ttgctatggg
     8821 tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt
     8881 tacaccattg tcattaggta tcgaaacaat gggtggagtg ttcactaaat tgattgaccg
     8941 caatacaacg attccaacaa gtaaatcaca agtattctca actgcggcag ataatcaacc
     9001 agcagtagac atccatgtat tacaaggtga acgcccaatg gctgctgaca acaaaactct
     9061 aggaagattc caattgacag atattccagc agctcctcgt ggtattccac aaatcgaagt
     9121 aacattcgat attgataaaa acgggattgt aaatgtatct gcgaaagatc taggtacaca
     9181 aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg
     9241 tatggtaaaa gatgcagaag caaatgcaga agcagataaa gctcgtaaag aggaagttga
     9301 cctacgtaat gatgtagatg cattgctatt ctctgtggac aaaacattga aagaattaga
     9361 aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc
     9421 cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat
     9481 cgttcaaaac ttgactgtta aactatacga acaagcggca caacaacaag ctcaagaaaa
     9541 cccagaagca gctcaaggcg gagcagatga tgtcgttgat gctgattttg aagaagtcga
     9601 tggcgacgat aaataatcag ttcactgaat ttcgagagaa ggccaaagtt gctactttgg
     9661 ctttttcttt tgaaaaatag tttgaagtat cggtttttgt ttcaaacaaa atatgatatg
     9721 ataacaagcg acgtgaaaat gatgagaacc aataggagga aagcctatgg caacaaaacg
     9781 tgattattat gaagtcttag ggctctcaaa aggagcatca gaagatgaaa taaagaaagc
     9841 ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat aaagaacctg atgcggaaga
     9901 aaaattcaaa gaagtatcag aagcatatga gattttaagt gacccgcaaa aacgcgctgc
     9961 atatgaccaa tacgggcatg caggcacaga tccaaattac ggtgctggtg gagctggtgg
    10021 cggatttggt ggattcggcg gcttctctgg tggcggcttc ggtggttttg aagatatctt
    10081 cgactctttc tttggaggag gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc
    10141 tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg atttttggtg tagaaaaaga
    10201 aatcaagtat aatcgggaag aaatctgtca tacttgtggt gggaacggtg caaaaccagg
    10261 cacacaacca actacttgtc ataaatgtca tggtgcaggt acgattaatg tagaacgcca
    10321 aacaccactt ggacgagtga tgagccgtca aacttgtgat gtttgtcatg ggacagggaa
    10381 agaaatcaaa gaaccttgtc caacctgtca tggctcagga catgagaaga aagcacattc
    10441 agttaaagtg aatgtaccag ctggtgtaga agaaggacaa caaatgcgtt tagctggtca
    10501 aggagaagct ggagaaaacg gcggaccatt tggtgatttg tatgtggtct tccgtgtaga
    10561 agaaagtgat atctttgatc gtgaaggttc tgaaatttac tatgaattgc ctttaagttt
    10621 tgtacaagca gctttggggg atgaagtcca agtaccaact gtccatggag atgtgaaatt
    10681 gaagatccct gctggaacac agacaggtac gaacttccgt ttacgtggaa aaggcgctcc
    10741 gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa gtaaaactga tcacaccgaa
    10801 aaatctaaat gaacaacaaa gagaagcatt acgtgcattc gctgaagctg gcggcatcaa
    10861 agttgaagaa cagcaagaag acggtttctt cgataagttt aaagatgctt ttggcgggaa
    10921 aaagcggaag tagtttttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
    10981 acaatcatat gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca
    11041 gctttagaat atgataatcg tcatagtttc taggggtata accagtgaga gaatggaact
    11101 tgttcaatcg atattgcaag gtgtttttat gaataaataa ttcttctgag caatttttga
    11161 tgcttccgtt atttttactg aagcatgcca aagtcttctt gaacaaatgt acttcttctt
    11221 ctgtcaggct tttcaaaata cgttttacat ataatgttcg ttgatctgac gggattgctg
    11281 gtaaaacgat cccgaggtct aaatcttcaa aaaagcggat ctcgttcaca gattgatgga
    11341 aagctatcca ttcgcaaact tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg
    11401 tataacaaag cgacccaatt ccaaagttta gctgtaactg atacatttta ttaaactttg
    11461 tctggatttt atcaagcaat gatagcaatg aggctctgga aatccgattg actaggataa
    11521 tgatttgttg tctatgtaca gtgagcaaag cctgtccgta ataagagaag accctttcga
    11581 gcaactccaa taaaggattg atccgttctt gacacaattg ctgattgtca gaaagttgtc
    11641 ccacaactac ataatatgga gaagaaaaat caagctcaat taagtgaggg tggtatcgac
    11701 tatgtacttg atatcatcaa tgaaccgatg gtactttctt atgcgatgga acaggtagag
    11761 cgattgcttg tttgtgcagg aaaacaagcg atgcggctga tacgattagg tgagaaaata
    11821 agaagataaa aatacacggt cccttcatgg cgaccgtgca tttttggttt ttattaaata
    11881 tttaatactt tattcaagaa atcttttgtc cgttcatttt gcggattttc aaagatttgt
    11941 tgaggcgttc catcttctaa aaagtttccg tcgtcaatga acatgacgcg attggccact
    12001 tctttagcga accccatttc gtgcgtaacg ataaccatcg tcataccttg ttctgctaac
    12061 tttttcatga cgttcaagac atcaccgacc atttcaggat cgagtgcaga agtaggctca
    12121 tcaaaaagca tgatgtcagg gttcatcgct agagcacgag caatagccac acgctgtttt
    12181 tgtccaccag aaagcatttc aggatacata tcttttttat cttctagacc aactgtctgc
    12241 agtaatttga ttgctttttt ctctgcttct tctctagatt ctttcttcag gtccatcggt
    12301 gctaaaatga tgttttccaa aatagaaaga tgagggaata ggttaaagtg ttggaatacc
    12361 attccgatat gttgtctcac caagttgata tctgtgcttt tatccatcaa gtgggcgccg
    12421 tcaataataa tttcaccgtc agaaggttct tctaaacgat tcaagcaacg caagaatgta
    12481 ctttttcctg aacctgaagg gccaatgata cagacgacat ctccttcgtt gatggataca
    12541 ttgatgtcgt ttaatactgt gttatcgccg tattttttga ctaaatgctc aactaagatt
    12601 ttttctgcca tcttacttca ccttcttttc taaaactttt gctaattttg tcaatgctgt
    12661 gatcaaaatc aagtacatga tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg
    12721 tgcaacaatg atcttaccag tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt
    12781 tgtgtctttt aatgagataa cgaactgatt gacgaatgat gggatcatga tgcgtatcgc
    12841 ttgcggcaaa atgatttttt gcattgtccg attataagaa agacctaaac ttctggaagc
    12901 ttccatttgt ccgacaggaa cggcgttgat cccgcctcga acaatctctg cgatataagc
    12961 acttgcgtta agagtcaagg tgataacacc agcaaggaag tcaggaatcg taaatccaac
    13021 gataccaggc aacccaaaga aaataaagaa cgcaagaacc atcattggaa ttcctcggat
    13081 gatatcgaca taaatactag caattgtgcg aagagcgcgt attggtgcta cactgaataa
    13141 tccaaaaatc acaccgatta ctatagccaa agcaaaggag atcaaagtta ggagaattgt
    13201 tctccaaagt cctttcaaca atgcttgata gttgttttgg ataagtccaa gaaatgttga
    13261 ttcatcagca gtggaagact cactggaagt ttctgaacca tccgctacat attctttaat
    13321 aatcttatcg tattcgcctg ttctttttaa ttctttcaat ccttcattga acatattaag
    13381 aagctcagga ttttgtcctt ttttaacggc aaatgcatat tttccgccag attctttttt
    13441 gatcggtgtt tccaattctt gtccttgttt gatcgcataa ccgattaccg gataatcatc
    13501 catcattgca tcgattgcgc caacttttaa cccgtcgtac aattgatcag cagcatcaaa
    13561 atatttgata gaatagccat attcagattc atggtcgctt aaaaattcag cactttcggt
    13621 accaactttt gctccgactg tttttccttt caaatcttca taagacttga tcgaagtatc
    13681 tccttttttg acagctaatt ggatcccgct atcaaagtaa gaatcagaaa aatcatagct
    13741 tttctttcgt tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc
    13801 caaagcttgc aaagcagcag agaaaccacg gtagtccatt cggatgttaa atccttgcag
    13861 tttggcgatg ttttccatca attttacatc gatgccttcg tactcgccct ttgcgttttg
    13921 aaattcaaat ggagcaaaag cagagtcact ggcaattacg tatacgtctt ttttgggctc
    13981 tattttttgc attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc
    14041 aacatgacca aacgcaaatg tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa
    14101 atgctttctt ttcataatca acctcctaaa tttccaatat tttattatta taggcgaaaa
    14161 gtgctgaatc agacaagact gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat
    14221 tttatgttat aaaacataac atgtataata agataaaaaa gtggcgaaag tatgttcggt
    14281 gtggtataat tttttagtaa ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa
    14341 atgaaaagga atgaatgaaa aggaggacga agaatgattg agagagatac ctcccgacac
    14401 tttgagcttg tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat taaccaatta
    14461 gtggatggcg tagtcggcgg gaaaaaagca caaatcttat taggtgcgac tggaacaggg
    14521 aaaacatata cgatttccaa tcttattgag aaagtgaata aaccaacact gattattgcc
    14581 cataacaaaa cattggctgg tcagctgtac ggggaattca aggaattttt ccctaacaat
    14641 gctgttgaat attttgtttc ttattatgat tattatcagc ctgaagctta tgtgccttca
    14701 agcgatacgt atatcgaaaa agattcaagt gtaaatgatg agatcgacaa attacgacat
    14761 tcggctacta gttcattatt agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt
    14821 atttttggat taggctcgcc ttttgagtac caaaaacaag tagtttcgat tcgacaggga
    14881 gcagaactag atcgcaatca gttgattcgt gacttagtca gtatccaatt cgaaagaaat
    14941 gatatagatt ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg
    15001 gcttcaagag atgaacgtgc cttacgtgtg gagttttttg gagatgaggt cgaacgtatc
    15061 cgagaagtca atgcactgac aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc
    15121 ccggcgactc actttgtgac gaatgatgaa catatggagc atgccgtagc gaatatcaaa
    15181 gcggaattag aacagcgttt aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa
    15241 cgtttagaac agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg
    15301 ggaattgaaa attattctcg gcatatggac ggacgtaaag aaggtgaacc gccttatacg
    15361 ctattggatt tctttcctga agattttttg atcgtggcgg acgaatcgca cgtaaccatg
    15421 cctcaaatca gaggaatgta taatggtgac cgagcgagaa aacagatgct agtagattac
    15481 ggtttccgtt tgccatctgc tttagacaat cggccacttc ggttagaaga gtttgaaaaa
    15541 catgtcaatc agatcattta tgtctctgcg acgccaggac catatgaaca tgaacaaaca
    15601 gataccgtta tccaacaaat catccgtccg acagggctgc ttgacccagt gatcgaagtt
    15661 cgtccaatca tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa
    15721 gaccagcggg tgtttgtaac gacattgacg aaaaaaatgg cagaagacct cacagattat
    15781 ttcaaagaat tagggatcaa agtcaaatat ttgcatagtg atatcaaaac gctggaacga
    15841 acagaaatca ttcgagatct tcgattaggt gaatttgatg tgttagttgg aatcaatttg
    15901 cttcgtgaag gattagacgt acctgaagtt tcccttgttg ctatcttgga tgcggataaa
    15961 gaaggctttt tacgtagtga acgttcctta gtccaaacga ttggacgagc tgctcggaat
    16021 gaagaaggta aagtaatcat gtacgcagac aaagtaacag attctatgcg tctagcgatg
    16081 gatgaaacgt caagacgtcg tacgattcag cagaagtaca atgaagaaca tgggatcgtt
    16141 ccaaaaacga tcattaaaga aatccgagat ttgatttcta ttacgaaaga atcggaagat
    16201 gatacaaaag aagcggtaca agtctcgtac gaagaaatga cgaaggaaga aaaagacacg
    16261 ttattgatga aattagaaaa agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca
    16321 gccgcgaatg tgcgagatat gattttagaa ttaaaagctt caaagtagga ggacaacatg
    16381 gctaacgata aaattacgat tcatggtgca cgtgcccata acttgaaaaa tatagatgta
    16441 acgattccac gagataaatt cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc
    16501 ctagcatttg atacgctgta cgctgaaggg cagcggagat acgtagagag cctttctgct
    16561 tatgctcgtc agtttttagg acaaatggat aagccggacg tagatagcat cgacggattg
    16621 agtccggcga tttctattga tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt
    16681 actgtgacgg aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg acatccgatt
    16741 tgtcccaacg atcatatcga gattaccagt caatcagtag atcaaatggc agatcaagta
    16801 atggctttgc ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa
    16861 ggacaacata aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc
    16921 gatggtgaga tctatgattt aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat
    16981 atcgcgatcg tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg cctatttgat
    17041 tctttagagg cggccctgcg attagcagac ggctatgctc tggttgacgt gatcggtgaa
    17101 gaagaaatgt tgtttagcga acactatgcc tgtccttatt gcggatttac tgttggcgaa
    17161 ctagaaccaa gactgttctc attcaatgcg ccttttggag cttgtccaga ctgtgatggt
    17221 ttaggtgtca aattagaagt cgatcaagat ttagtgattc cagatagaac gaagacactt
    17281 agtcaaggtg ctatcgttcc atggaatcca atcagctctc agtattatcc tcaaatgtta
    17341 gcgcaggctt gtgaaagttt cggtatcgat ttagatacac cattcgaaga attaccggaa
    17401 gagcatcagg agatcatctt gaatgggaca gatgatcttt tccatttcca ttatgaaaat
    17461 gattttggtg gcgttcgcga tgtagaaacg actttcgaag gggtattgaa gaatatcgag
    17521 cgtcgttatc atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca
    17581 gaattaactt gtcagacatg taaaggttat cgattgaatc cccaagctct atctgttcaa
    17641 atcaatggaa cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc
    17701 ttctcaaact taacgttatc tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa
    17761 gtaaatgaca gactttcatt cttggaaaat gtgggactag actatctaac tttgagtcgc
    17821 gcttccggaa cattatctgg gggagaagcg cagcgaatca gattggctac acaaattgga
    17881 tcgaatcttt ctggagtttt gtatatttta gatgaaccat cgatcggatt gcatcaacgc
    17941 gacaacgatc gactgattgg ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt
    18001 gtagtcgaac atgatgaaga tacgatgtgg gcagcagatt atttgattga cgtcggacct
    18061 ggggccggag aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga agtagctaat
    18121 gatgaaaatt ctttgacagg gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa
    18181 gaacgccgta aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga aaacaatctg
    18241 aaaaatatta gtgttgaatt tccattagga gaatttgtag cagtcacagg tgtgtctggt
    18301 tctggaaaga gtacattggt caatagtatc ttaaagaaat cgttagcgca aaaattaaat
    18361 aagaattctg ctaagccagg taaattcaag acaatttccg gctacgaaag tatcgaaaag
    18421 atcatcgata tcgatcaaag cccaatcggc cggacgccga gaagtaatcc agcgacttat
    18481 acaagtgtat ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg
    18541 ggttataaga aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc
    18601 ggggatggaa ttattaagat cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa
    18661 gtatgtcatg gtaaacgata taattctgaa acattagaag tgcattacaa aggaaaaagc
    18721 attgctgata ttttggaaat gacagtagag gatgctgtag aattcttcaa gcacattcca
    18781 aagattcatc gcaaactgca aacgattgtt gatgttggct taggttatgt gacgatgggg
    18841 caaccagcaa cgacattgtc cggtggtgag gcacaacgga tgaaacttgc cagtgaattg
    18901 cacaaaatct ctaatggaaa gaatttctat atactagatg aaccaacgac aggacttcat
    18961 agcgatgaca tcgcccgctt gttgcatgta ttacaaagat tagtagatgc tggtaacaca
    19021 gttttagtga ttgaacacaa tctagatgta atcaaaacag cagattatat cattgattta
    19081 ggaccagaag gtggagaagg tggaggaacg atccttgcga ctggaacacc agaagaaatc
    19141 attaacgtaa aagaaagtta tacaggtcac tatttgaaaa aaataatggt ataaagagga
    19201 tagatagctg agatagttaa aaaaacataa aaa
//
LOCUS       DAPCMU010000043        19108 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPCMU010000043 DAPCMU010000000
VERSION     DAPCMU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.044"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..703)
                     /locus_tag="RD744_001242"
     CDS             complement(<1..703)
                     /locus_tag="RD744_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557253.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="RD744_001243"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="RD744_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="RD744_001244"
     CDS             1758..2837
                     /locus_tag="RD744_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDU0557254.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="RD744_001245"
     CDS             2869..3462
                     /locus_tag="RD744_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557255.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="RD744_001246"
     CDS             complement(3572..3799)
                     /locus_tag="RD744_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0557256.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="RD744_001247"
     CDS             complement(3902..6490)
                     /locus_tag="RD744_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDU0557257.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="RD744_001248"
     CDS             complement(6510..7169)
                     /locus_tag="RD744_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0557258.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="RD744_001249"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="RD744_001249"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDU0557259.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="RD744_001250"
     CDS             complement(7942..8808)
                     /locus_tag="RD744_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0557260.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="RD744_001251"
     CDS             8917..9558
                     /locus_tag="RD744_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDU0557261.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="RD744_001252"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="RD744_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDU0557262.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="RD744_001253"
     CDS             complement(10999..11898)
                     /locus_tag="RD744_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDU0557263.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="RD744_001254"
     CDS             complement(11900..12697)
                     /locus_tag="RD744_001254"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDU0557264.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="RD744_001255"
     CDS             complement(12675..13379)
                     /locus_tag="RD744_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDU0557265.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="RD744_001256"
     CDS             13510..14085
                     /locus_tag="RD744_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDU0557266.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="RD744_001257"
     CDS             14203..14880
                     /locus_tag="RD744_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDU0557267.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="RD744_001258"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="RD744_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDU0557268.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="RD744_001259"
     CDS             complement(16943..17941)
                     /locus_tag="RD744_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDU0557269.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="RD744_001260"
     CDS             complement(18167..18820)
                     /locus_tag="RD744_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDU0557270.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5519 a         4134 c         3322 g         6133 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaa
//
LOCUS       DAPCMU010000044        19032 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPCMU010000044 DAPCMU010000000
VERSION     DAPCMU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.045"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(43..1611)
                     /locus_tag="RD744_001261"
     CDS             complement(43..1611)
                     /locus_tag="RD744_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDU0557271.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1615..1896)
                     /locus_tag="RD744_001262"
     CDS             complement(1615..1896)
                     /locus_tag="RD744_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDU0557272.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1893..2816)
                     /locus_tag="RD744_001263"
     CDS             complement(1893..2816)
                     /locus_tag="RD744_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDU0557273.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2813..3691)
                     /locus_tag="RD744_001264"
     CDS             complement(2813..3691)
                     /locus_tag="RD744_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDU0557274.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3678..5015)
                     /locus_tag="RD744_001265"
     CDS             complement(3678..5015)
                     /locus_tag="RD744_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDU0557275.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5018..6121)
                     /locus_tag="RD744_001266"
     CDS             complement(5018..6121)
                     /locus_tag="RD744_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDU0557276.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6195..7703)
                     /locus_tag="RD744_001267"
     CDS             complement(6195..7703)
                     /locus_tag="RD744_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0557277.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7669..8361)
                     /locus_tag="RD744_001268"
     CDS             complement(7669..8361)
                     /locus_tag="RD744_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0557278.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8383..9357)
                     /locus_tag="RD744_001269"
     CDS             complement(8383..9357)
                     /locus_tag="RD744_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557279.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9657..10529
                     /locus_tag="RD744_001270"
     CDS             9657..10529
                     /locus_tag="RD744_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0557280.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11111..13309)
                     /locus_tag="RD744_001271"
     CDS             complement(11111..13309)
                     /locus_tag="RD744_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557281.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13541..14533
                     /locus_tag="RD744_001272"
     CDS             13541..14533
                     /locus_tag="RD744_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0557282.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14593..16014
                     /locus_tag="RD744_001273"
     CDS             14593..16014
                     /locus_tag="RD744_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDU0557283.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16007..17506
                     /locus_tag="RD744_001274"
     CDS             16007..17506
                     /locus_tag="RD744_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDU0557284.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17545..18939
                     /gene="uxaC"
                     /locus_tag="RD744_001275"
     CDS             17545..18939
                     /gene="uxaC"
                     /locus_tag="RD744_001275"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDU0557285.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5714 a         3877 c         3366 g         6075 t
ORIGIN      
        1 agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc
       61 atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac
      121 tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg
      181 acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt
      241 ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat
      301 ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca
      361 agtaacataa caataaatat aattggattc atttaagtac agtaccagtc cagccatctg
      421 attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg
      481 ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg
      541 taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc
      601 attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg
      661 ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt
      721 aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa
      781 aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg
      841 ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc
      901 tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca
      961 aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc
     1021 gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt
     1081 atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct
     1141 ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc
     1201 cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc
     1261 ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg
     1321 acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat
     1381 ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa
     1441 aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg
     1501 aagccattca aaagaggaga caataatgta ataccgctct ttcactctta atatattagg
     1561 atctggattg aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc
     1621 tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa aagcaagtga
     1681 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     1741 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     1801 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     1861 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     1921 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     1981 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     2041 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     2101 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     2161 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     2221 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     2281 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     2341 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     2401 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     2461 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     2521 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     2581 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     2641 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     2701 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     2761 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     2821 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     2881 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     2941 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     3001 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     3061 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtgttt
     3121 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     3181 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     3241 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     3301 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     3361 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     3421 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     3481 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct tctttttctg
     3541 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     3601 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     3661 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     3721 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     3781 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     3841 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     3901 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     3961 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     4021 atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg ataagccttt
     4081 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     4141 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     4201 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     4261 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     4321 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     4381 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     4441 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     4501 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     4561 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     4621 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     4681 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     4741 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     4801 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     4861 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     4921 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     4981 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     5041 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     5101 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     5161 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     5221 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     5281 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     5341 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     5401 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     5461 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     5521 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     5581 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     5641 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     5701 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     5761 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     5821 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     5881 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     5941 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     6001 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     6061 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     6121 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     6181 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     6241 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     6301 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
     6361 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
     6421 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
     6481 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
     6541 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
     6601 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
     6661 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
     6721 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
     6781 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
     6841 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
     6901 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
     6961 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
     7021 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
     7081 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
     7141 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
     7201 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
     7261 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
     7321 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
     7381 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
     7441 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
     7501 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
     7561 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
     7621 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
     7681 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
     7741 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
     7801 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
     7861 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
     7921 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
     7981 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
     8041 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
     8101 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
     8161 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
     8221 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
     8281 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
     8341 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
     8401 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
     8461 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
     8521 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
     8581 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
     8641 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
     8701 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
     8761 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
     8821 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
     8881 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
     8941 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
     9001 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
     9061 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
     9121 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     9181 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     9241 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     9301 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     9361 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     9421 ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg atttttgtgc
     9481 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     9541 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     9601 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     9661 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     9721 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     9781 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     9841 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
     9901 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
     9961 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    10021 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    10081 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    10141 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    10201 gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact atctcgcttt
    10261 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    10321 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    10381 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    10441 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    10501 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    10561 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    10621 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    10681 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    10741 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    10801 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    10861 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    10921 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    10981 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
    11041 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
    11101 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    11161 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    11221 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    11281 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    11341 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    11401 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    11461 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    11521 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    11581 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    11641 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    11701 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    11761 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    11821 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    11881 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    11941 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    12001 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    12061 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    12121 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    12181 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    12241 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    12301 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    12361 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    12421 gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc agtgttaaag
    12481 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    12541 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    12601 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    12661 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    12721 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    12781 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    12841 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    12901 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    12961 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    13021 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    13081 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    13141 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    13201 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    13261 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    13321 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    13381 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    13441 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    13501 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
    13561 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    13621 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    13681 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    13741 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    13801 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    13861 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    13921 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    13981 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    14041 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    14101 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    14161 caatctagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    14221 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    14281 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    14341 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    14401 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    14461 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    14521 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    14581 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    14641 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    14701 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    14761 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    14821 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    14881 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    14941 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    15001 cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    15061 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    15121 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    15181 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    15241 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    15301 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    15361 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    15421 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
    15481 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    15541 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    15601 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    15661 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    15721 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    15781 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    15841 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    15901 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    15961 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    16021 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    16081 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    16141 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    16201 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    16261 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    16321 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    16381 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    16441 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    16501 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    16561 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    16621 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    16681 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    16741 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    16801 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    16861 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    16921 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    16981 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    17041 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    17101 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    17161 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    17221 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    17281 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    17341 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    17401 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    17461 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    17521 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    17581 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    17641 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    17701 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    17761 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    17821 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    17881 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    17941 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    18001 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    18061 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    18121 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    18181 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    18241 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    18301 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatctt
    18361 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    18421 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    18481 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    18541 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    18601 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    18661 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    18721 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    18781 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    18841 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    18901 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    18961 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    19021 cggaccaaat tt
//
LOCUS       DAPCMU010000045        18736 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPCMU010000045 DAPCMU010000000
VERSION     DAPCMU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.046"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            30..1457
                     /locus_tag="RD744_001276"
     CDS             30..1457
                     /locus_tag="RD744_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557286.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1549..1914)
                     /locus_tag="RD744_001277"
     CDS             complement(1549..1914)
                     /locus_tag="RD744_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDU0557287.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1911..2276)
                     /locus_tag="RD744_001278"
     CDS             complement(1911..2276)
                     /locus_tag="RD744_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDU0557288.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2468..2716)
                     /locus_tag="RD744_001279"
     CDS             complement(2468..2716)
                     /locus_tag="RD744_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557289.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2715..2843
                     /locus_tag="RD744_001280"
     CDS             2715..2843
                     /locus_tag="RD744_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDU0557290.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2900..4594
                     /locus_tag="RD744_001281"
     CDS             2900..4594
                     /locus_tag="RD744_001281"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDU0557291.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4598..4717
                     /locus_tag="RD744_001282"
                     /pseudo
     CDS             4598..4717
                     /locus_tag="RD744_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5245..6378
                     /locus_tag="RD744_001283"
     CDS             5245..6378
                     /locus_tag="RD744_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDU0557292.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6409..6729
                     /locus_tag="RD744_001284"
     CDS             6409..6729
                     /locus_tag="RD744_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDU0557293.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6758..7222)
                     /locus_tag="RD744_001285"
     CDS             complement(6758..7222)
                     /locus_tag="RD744_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557294.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7354..8607)
                     /locus_tag="RD744_001286"
     CDS             complement(7354..8607)
                     /locus_tag="RD744_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557295.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8636..8953)
                     /locus_tag="RD744_001287"
     CDS             complement(8636..8953)
                     /locus_tag="RD744_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0557296.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8979..9299)
                     /locus_tag="RD744_001288"
     CDS             complement(8979..9299)
                     /locus_tag="RD744_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0557297.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9763..11178)
                     /locus_tag="RD744_001289"
     CDS             complement(9763..11178)
                     /locus_tag="RD744_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0557298.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11195..12511)
                     /locus_tag="RD744_001290"
     CDS             complement(11195..12511)
                     /locus_tag="RD744_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557299.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12661..13359)
                     /locus_tag="RD744_001291"
     CDS             complement(12661..13359)
                     /locus_tag="RD744_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDU0557300.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13648..14604)
                     /locus_tag="RD744_001292"
     CDS             complement(13648..14604)
                     /locus_tag="RD744_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0557301.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14717..15550)
                     /gene="msrA"
                     /locus_tag="RD744_001293"
     CDS             complement(14717..15550)
                     /gene="msrA"
                     /locus_tag="RD744_001293"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDU0557302.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15725..17359)
                     /locus_tag="RD744_001294"
     CDS             complement(15725..17359)
                     /locus_tag="RD744_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557303.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17346..18731)
                     /locus_tag="RD744_001295"
     CDS             complement(17346..18731)
                     /locus_tag="RD744_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557304.1"
                     /translation="MCTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
BASE COUNT         5922 a         3696 c         3135 g         5983 t
ORIGIN      
        1 gcaaaaaaat acaaggcgga ggctctacta tgattgaaga atatattgag aaaaatattt
       61 taagacagct gtttttatgt gggcaattct atgtaaataa agaagtcaat ctagaaaagc
      121 tctccaatct cctgcatgtt tgcaaaacca ctttactcaa tgatatcaat aacataaaaa
      181 aagaatttga agaacagatc gcttatacac atcgggaaaa ggaccgttac actttatatt
      241 tttcagaaca tatacctcga tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt
      301 taaaaacttg tctgctttat ttggaagaag atgaacctga ttatcttcaa ttaactgaat
      361 gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa acaagtatta gcttacttta
      421 atgactgcgg gatcgaaatc gaccgctact cccctcgatt cactgaaatg gaaagacgtc
      481 tgctgcttct taatgtgtcg tatcgtttag gcggttttaa ttcttgggag ctacctgaat
      541 cgttttttga acgtgccgat cgtttcattg aatccgtaac ggaaaatagt ggcagatttt
      601 atgataaaga aaataaagaa atcttatcga ttggttttgc tatcagtttc ctacgccagc
      661 aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt
      721 acaattatgt cgaatcagca tgggaaaaca ctgatttcca aacctattat aaaaaagaag
      781 aattcgcctt tattttgaca ctattcaatc tttgtaatta tggtttccat tcttatcagt
      841 tgattgcgga agactttcaa cagctacacc aagtgtttat tgacaatact cccgaaataa
      901 aagaactagt tgctaccttt gaatctcact tcaatcaaga attattcgga aaccaaccgt
      961 tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga taactatcaa ttattcatgc
     1021 cagaaaaatt ttatctttta aatgaggaac agacaaattt atataaagaa gtccagacaa
     1081 ttttttccag ctggtccagc cagttacctt atgatctacg gttgaatcct aattgcatgc
     1141 gggcttttgt gatcgaattg agcggaattc tccgtctgac aaaagaacat ctgactatct
     1201 atatcgtcac aaattcggat gttcattatc tgatttatcg ggaagccttg gaagctgtaa
     1261 ctactttcga ttttcaagtt gcaccaacaa tttattcaag tatatctgat ataaagaagt
     1321 atgcgcagca gtcctcaaac cgggtattat gtgaacgtac attatataca ccagatgcgg
     1381 tccaatatga aaatattatt cctatttcga tcaatacgat cgatcgagct atcatttcag
     1441 ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc acagcctctt atttgttgaa
     1501 gatgcccatt tctgtaacaa tctcttgata cacgtcaggc tcacaacctc acttattttg
     1561 gacatctagg ggtccgatct ctttatttaa aaaataagaa atgatggttc ggataactac
     1621 caacgccgct agttggaaaa tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc
     1681 agctgaaaca agaatttcca aacttagaag tatatagcct cccaacatct ttttaatagc
     1741 attgttctcg cttactcggt cgctttgcac ttttgtatgg acaaataaat atttcaaaaa
     1801 atccttgatt gatagacaaa caccccaaac caataaacag atagcaaaga tatcaagtcc
     1861 cagtacaagt ttttcgaaat atggaagaag aaactgcatc aactctgtcg tcatcctacc
     1921 tctcctcttc tttgtttttc tttgtcttct atttcctgat tcaagaaata agaaatgact
     1981 gttcgaatga taataataag tgcaagcttc aaaatatctg tccaagtcgg cttgataact
     2041 gactcgatga tatcagcaac gactagtact tccaagctta acaaaatata acttgctagc
     2101 agttttcttg tatctgcatt acgagtatta cgttctgcat agtttttctt gacatgagac
     2161 caatcaaaaa gtttgaatag aacaagaacg atcccgacaa ttaagatacc gatagaacaa
     2221 atatttaaaa tagcaatgat ccattctaga aattcaatta aatttgagcg aaacatatac
     2281 gctacctact ttcttttaat ttgttcctat tcttttattg taaaagaaag ttaccttgtt
     2341 cgtctattta aaagttgttc taatgataac ggttcgctta aatttttctt tttcactctc
     2401 ccatttttct ccctccatcc ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa
     2461 ataaggctta gtccgtcatc agcttttcaa agaagcactg tctcggtgct ttttttgtac
     2521 ccaaatttct aaaaatagaa ccatacacct cctcaaatct tttcttctta cctaccctat
     2581 ttttatttac aggaaaatca gacacttctt cttttttgtc aaaatttcct ttttcttctc
     2641 gtcctatcat ttggcattct aataaaaacg aactatcctt ggatatagaa agagaaaaca
     2701 aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg
     2761 cccttggcgc tgcatatcta ggaaaaaaga caggactatt cgaagatgac agtcatttat
     2821 atgatgaatt cgaatcaatc tagcatccta tttagttgaa tcactacgga catacttgga
     2881 agacattgga ggaatcacaa tgagaaaaag taaagcaata atgattggtg ccggtattgc
     2941 caatatggca gcggctgttt atttgattca agaaggcaaa tggaaagggg atcagatcac
     3001 cttttattca ttggatgatc acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga
     3061 tgaatattgg aataaaaacc atccattaga aaaccaaaaa ggctatattg ctcgaggcgg
     3121 tcgcatgctt aattaccgta cctatgtaga tttgatggat ctgttaagtc gaataccttc
     3181 tgttactgag ccagggatga ctgctgaaga agatacgaga gattttgatg caaaacaccg
     3241 aacctttgac aaagcacgtt tgatggaagg tggcaaagga atcattgatg gcggaaaact
     3301 gggattcaat aataaagacc gtattcttct gactaaactg attgctatgc ctgattcaga
     3361 agaagaaaaa ctagacaata tcactatcga agaatatttc aaagatgatc cacacttttt
     3421 tgaaacaaac ttctggttta tgtgggaaac aacatttgct ttcagaacac gtagttctgc
     3481 tcaagaattg cgtcgctata tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct
     3541 agtgggcgtc aatcgaacac gttacaatca atacgaaagt attatgcttc ctttgatcga
     3601 gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt
     3661 caaagaaaca ccgatgcaag atgaaatcac tgtgactggt ttgaaaatta ccaatacact
     3721 gactcaagaa gaagaatacg ttgtggtcga tgaagataca gcagttattt tcactaatgg
     3781 gtccattaca gattcagcta ctttaggaga ttacgaaaca ccagctgtag aaaatatgga
     3841 ttatggtgca gcttctagtc tctggaaaaa agccagtgaa cgtttctaca acttgggaaa
     3901 tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg gtcagcttta ccctgacgac
     3961 gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg actcaagaac caggaaatgc
     4021 cttgaattca tttatttcaa ccacaccgat cacgccgtta ggccaaaaag atgtcaatat
     4081 gtctattgtc gtgcatcatc aaccgcattt tacgtctcag aaaccaaatg aaacagttat
     4141 ttggggttat ttcctttatc caagacgacg tggcgaattt gtggataaac agtatattaa
     4201 aatgaatgga aaagaaatgc tagaagaact gattggacaa ctttcaaaag tagatcctgg
     4261 accagtgaat attcgtgaaa aagaaacaga aattttcgac agcgtaataa ataatatccc
     4321 tgtctatatg ccttatgctt ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa
     4381 agtcattcct aaacattcga ccaatctggc ctttacagga gaatttgtcg agcagcctta
     4441 tcagatgatc tttactgaac aaagtgctgt acgttctgga gaaatcgctg ctttccattt
     4501 tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt tacgacaaag atattcctac
     4561 tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga ggttgtgaaa aagctgccct
     4621 gcatcaagta ataagaacaa ccaggctaaa acagcttctt atgtttttga tttggttgga
     4681 cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg aaaaagctgc
     4741 cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt tgacttggtt
     4801 agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagg
     4861 cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa
     4921 atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta tttatgtctg
     4981 tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc aacatcatct
     5041 aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac acacctctcc
     5101 gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt ttcattatca
     5161 aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc agacaatttt
     5221 atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg
     5281 gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat catccgtatc
     5341 acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga agcagttttg
     5401 gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt gggatctcaa
     5461 gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag ttgtggtcat
     5521 tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc agatcagtta
     5581 tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg tcaagcggaa
     5641 tatctaagag ttccttatgc agactctact tcatttgttg taccagataa tgatcttcct
     5701 gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag cgttgaaaac
     5761 gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt cggtctattc
     5821 gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga tccagtacag
     5881 caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt agaagacacg
     5941 gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt cattatcgat
     6001 tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt agtaagctta
     6061 caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa atttggaaca
     6121 gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca agaatttttt
     6181 atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat gccaaaaatt
     6241 tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca ttcgctgcca
     6301 ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa aaatataaaa
     6361 gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat gcccaaaatc
     6421 aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat acgaggacgt
     6481 cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt cgatttatta
     6541 accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc cgatcgatat
     6601 ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat tctcagaatc
     6661 gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa
     6721 gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta tcactttgat
     6781 acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc cactcgtatc
     6841 gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca agttgatgca
     6901 tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc ttttccggtt
     6961 caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag tcgttttcaa
     7021 tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac cattgaggaa
     7081 tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca gcaggaactc
     7141 cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt
     7201 tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg tttacaaaaa
     7261 ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg
     7321 aaaacaatca cttgttctcc gttttttgat taatcaagca tctaaagctt cttttcttct
     7381 ttcttggatc gtagctacct tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt
     7441 gacagcggct agtacaccac ctgcaatact ttttgttgcc aatacaccgc caataattgg
     7501 tggagttacc catggaggca taacggtagc tgctggcact aaacctgtag aagtcgcaaa
     7561 gtaagcaaca ctaactaaga ccattggtgt caaaataaat ggaatgaaca tgatcgggtt
     7621 caacacgata ggtaacccaa acatcgttgg ttcattaata ttgaagacac ctggcgcaat
     7681 cgacaagttt gttacaacca tatatggttt gtttttacgt cctactaaat aaatagctaa
     7741 cagtaaacct aaagttgcac ctgttcctcc taagttgaca aagctgtcaa agaaaggttt
     7801 gttcacgata aacggtgcgc tatgtccagc tgcgattgca ttaacgtttg cttcaatggc
     7861 tggcgcattg attgtttgca tcaaaggatc gaccatattc gctccgtgta aaccaaagaa
     7921 ccataagaat ggtgtgataa atgcaagcag taaggcagat ggataagagt ttgcaagtcc
     7981 cataaatggt tcttggacca ctgtatagaa agaacctacg atatcatcaa caccgaatgc
     8041 agcaaaaatt gcagcaacta aagcaaacgc agagatagtg atcattgctg gtagtaatga
     8101 agcaaatgct tttgcaactg ccggtggcac accatcaggc attttgatga tcaatttttc
     8161 atttccgctt aaacgtacaa agatttctgt tgaaataatc ccaacaataa tagcaataaa
     8221 caaaccgttt gagctcatac ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat
     8281 cacacctgcg gaaacaccgt cttttcggta gccgttagct aaatgataag caagcaagaa
     8341 agcaatcaag acagaaagaa tggcaaatgt accgttccaa gcagcagccc cgaatccttt
     8401 ccatgattca ccaccaaaaa ttgagttcat caattcttgg aacccgggaa tcggtaagtt
     8461 attgatcatt actgctaatg ctcctaagat cattaacggc atactcacca taaatgagtc
     8521 ccgaatcgca actaaatgcc gttgtgcacc aatttttgat gctacaggta taaaatgttt
     8581 ctccataaat gcaacaaatt tatccataaa tcttccctct tttctttact gtagattacg
     8641 cgttcaatcg acgatacaga ttaatgatct cttttgctaa atctgtaaaa gctatagatg
     8701 tcatcagatg atcttgactg tggaccgtca aaagcgtgac ttcaacgtga tttccttgtg
     8761 cttcttgtgt cagcatctct gtttgagagt gatgtgcttg gactaaagat tcctctgcat
     8821 ctttgatttt ttcatctgct aaagtgaaat cacctttttt cgcagcagca atcgcttcca
     8881 ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc catgatggct tctagatttt
     8941 tttgatcttc catttattgt atcctccgat aatttttatt atttgtttat tagtttttct
     9001 gcttggtcga gtactttttc accattcatc atgccatagt cagccatatt gattacatct
     9061 aaaggaatgc ctttaggtgc tagcttttca gcaaattgat tttgcataaa gcgtacttgc
     9121 ggtcctaaaa gtaaaacgtc tacattttta gattctagtt gattatccgc atctgaagca
     9181 gatacagcaa agatgtctgc ttccattcct tttgcttctg ctgccttttg catttttgtg
     9241 actaggagac ttgtactcat tccagctgaa catacaagca tgattgtttt cttttccatt
     9301 ttaaacacgt tcctttctat acttacactt gtatataagc aagtagcgtg ccaactttaa
     9361 aagcgcttta aaaagttgat atcatcacat ttaaggtcta taccaagttg catacacact
     9421 aaaaaagaaa gcgcacacta aacacacaaa ttaattttta ttaccttttt tcttttaacg
     9481 tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt tttcaaataa acggtattca
     9541 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
     9601 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
     9661 tcaatcagct gaccctgggt attagctcaa aacgctggat tcacaacctc ttttatttca
     9721 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
     9781 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
     9841 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
     9901 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
     9961 ttcttctgtt gctttgatca agtaatgcaa atgctgtccg ataaattcga tacggtaatc
    10021 atcttgcacg attcctttct catccatata ctgttcttca tattctcttc ccataccgtt
    10081 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
    10141 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
    10201 aaagacttca taatatcgtt caggatggaa aggtgtctct tcattccatt gataacgagg
    10261 ggcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
    10321 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
    10381 caggaactct tctgtgtagt atccttttac catcggatca aaaaatactc gattataaaa
    10441 caaatcatac atataagcag ctttttggtc ttctggagaa ttggatcgag catagaccat
    10501 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
    10561 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
    10621 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
    10681 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
    10741 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
    10801 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
    10861 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
    10921 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
    10981 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
    11041 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
    11101 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
    11161 tggaatagta attttcatga tttcctcctg ttttttagat cccatcattt tccacacgat
    11221 ttgctacttt tacaaatggc agatagatca aaatagaaac aataatacaa acaagttgcg
    11281 taataactgc accgatattt cctgctgttg ccagataagc attgacgatc ggcggcattg
    11341 gccaagggac cattacaact gctttcgccg caaatcctaa agcggttgca aaataaccaa
    11401 tcgttccagt gataagaggc gttaagataa acggaatagc taaaattgga ttcagcatga
    11461 tcggcatacc aaaaataact ggttcattga tgttgaagat cccaggagca agggataact
    11521 tggtgatttg tttcatatct tcccgttttc caactaacaa tactgcaatc agtaaaccga
    11581 tcgtgactcc agacccgccc atactcatat acatatccca aaatggcata gtaataatgt
    11641 tcggtacttc ttttccagct tcaaaagctt ccgtatttac agcaattgcc gctaaaagca
    11701 acggttcccg tatcggcttc accatttgat ttccatggat accaatcacc caaaagactt
    11761 gcgcaataaa catcaatagc aaaatacccg gtaacccttg tacgacacct tctaatgggc
    11821 gttgtacgac attgtaaatg atatcataaa ggtacattcc tgtaagttct ttgatgataa
    11881 atcccgcagc cgcgataccc gatacagtca gaatcgtcgg gaataaagca gaaaaactag
    11941 cagaaacatt cgttggcacg gtatcaggca ttctcagctg tagtttcttc tgtttcctca
    12001 accagcaata aagttcaata gaaaggatgg agaccaacat tcccaaaaat aaccctttcg
    12061 tatctgtata ttgtttagcc aatacgtttt ctactataac agtctgattt tcattcacta
    12121 attcataaac agttggatta acagataaat aagacatcac tgcgatgata ccagggaata
    12181 caccatccac tttattcagc ttggctaatt cgatccctat cagaaaaaca gcatagatgg
    12241 tcaagaaact tagtgtgaca tagctgatgg cagatgcgat aggctttaat tcttctaaaa
    12301 aacgcaagaa acttatctgt gctaagccat tttccgagtc aaagaccatt gctgaaaaca
    12361 atgtgccaaa agcacccgta ataattaccg gaatcaatgc agcaaatgcg ttttttatca
    12421 ccatgatata ccttaaatta ttgattctcg ttgccacgtt ccctaaaaca ttggctaatt
    12481 tatccataaa actcattcca gatcccctca ttcctgcttc ttctccttga cgtcatctct
    12541 ttttgtctat tagtcagcgc ttacatttcg atgaacagaa actatttact cctctttttt
    12601 accaaatata aaacaaaaat tcatctgatg tatttatcat aaaatacatc agatgaactg
    12661 tcaattagtt tctttttctc ctattatttt ctgtaattct ctccgattta ttgccatatg
    12721 gacagtcatt gcatcaaacg cagcaagtgc attccgcctt tcgatggcct gaacgatctc
    12781 tgtatgaata tcgatcgtct cgattttcag atgctggata tcataatttt tattaaacaa
    12841 agcaattgat tcattaatga ttgggataat atgcagcaag gctacgtttc cgctagcttc
    12901 tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt tcttgtgtgc cttctcgaaa
    12961 agaagtttca atcttttgcg ttaatttttt catttggacg atttgattct tcgttgcata
    13021 ttccgctgcc aaacctgcca tcttaggctc caataaatat ctcagttcaa ataagtctcg
    13081 tatcatccga tcctgatctt caatcaaaga aaaaccgagt gggtcattgg caatccctcg
    13141 cttatcactg acaaatgtcc ccgctccttg ccgaacttca aggatattcc tggaagctag
    13201 agcccgcaca gcttctcgca ccgtgcttcg ccccacctct aaataatcgg acaattcata
    13261 ttcattcggc agtttatctc ctacttgcaa ctgcatttcc tttaaatatc taaggatctt
    13321 gtcagtagtt acttctacta gggttttatt ttcttccaaa ccacatacct ccttttcatc
    13381 tgatgtattt tttcgtaatt attataacac gtttgaatca tctgatgatt ttttgtatac
    13441 aaaattgatt aatccgttca tagacataca aacgaagaag atatttacca taaaaaagac
    13501 taggataaaa ttcactccta gccttcgatc atttttattt tgaacgacaa aaaatatgat
    13561 gttcaataac agacgatctt cattcgattc ttgatgtcat cttttgtttt ttttaatata
    13621 tggataaaaa gtttgattac atcgtttcta ttctagaatt tggtcgattc tcaattcttc
    13681 ttctggtgag aaatctaaat tatccatcat tcgaacactt tcttgtaact gacttaaccg
    13741 acttgctccg accagtacac ttgtaacaga tttttgtcgc aaattccatg caagggccat
    13801 ttgcgctaat gattgtcctc gtgatacggc gattgtttgc agtgctttga ttttttccag
    13861 agttgatcca acttgttctt ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc
    13921 ttcaggaata ccatgtagat aacgatttgt cagcaaccct tgagcaagcg gactgaacgt
    13981 gattgcccct agcccttctt cttctaatac tggagataga tcgtcttcta gtagtgcacg
    14041 agaaaacata ttgtagcgca tctgatgaat aatgaacggt gcttcctttc tttttaaaat
    14101 ttcagtcatt ttttttgtat cttctccatt atagttggat ataccaatat ataatgcttt
    14161 tccttgtctt acaagttgca tcaaagcttc tgctgtttct tccaaaggtg tatcagggtc
    14221 tgggcgatga tgatagaaga tgtcaacata atctagcccc attctttgga gactttggtc
    14281 acaacttgaa atgatggatt tacgagagcc ccattcccca tatggccctt cccacatatg
    14341 ataacctgct ttgctagaaa taatcagttc atcacggtag gcttgaaaat cttctctaaa
    14401 aatacgccca aagtttttct ctgcagctcc agcagggcct ccataattat tcgcaaggtc
    14461 gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc tctctttgat tatggatagg
    14521 atcataatca ccaaaatttt gccataatcc gagagataaa atcggtaatt tcaaacctga
    14581 atgaccggat ttacgatatt ccattttttc ataacgtgca ctatctgcaa tatacaattt
    14641 ttctcctcct catcaaatct ttttaaaacg ctttcttttt tctattttta gtttactaca
    14701 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    14761 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta taacgtttcg
    14821 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    14881 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    14941 gttccgctaa ttctttttgt cgttcgactt gataaaaaat cactggacga tattgcttcc
    15001 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    15061 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    15121 ctgtgtaacc accacttact tgatcataat ttggtttttc aacatgtccg cccgtgtatc
    15181 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    15241 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    15301 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    15361 caggcgtcag attcattcgg atagctaatg gacgtagtgt tgcttctagt tttgataact
    15421 gttctttaac ggatatattt tctcttatgt cattctttgt ctgaatcaat acttttcgtt
    15481 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    15541 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    15601 tatcataaag tgaggaaata agcgattact tcatccccct acagattttt cgtataaaaa
    15661 agagagaatc cttatcgaca gattctcctt tttaatcaat ttattttata aatgaaaagt
    15721 taatttacct tttatggaaa actattttaa aaaataaaca taaatatgca gttaatgtaa
    15781 caagataaga aataatagtc gttactgtat aacttgtgct tactttaata tcagcaactc
    15841 ctttgactcc gcttatttgt accaatccat tagcattcac ctgctccttt acaggttgat
    15901 gatttatttc tacattcgtt cctaaataag catatacagg aagtgtgatt acttggctct
    15961 tctcattagt tactctatat atgatagaac taccagttgt tttataagga aggtttaccc
    16021 attgactgcc aatctttgct tcgtgtttta ttatatgctc cttattctcc aatgaagcga
    16081 taggagaata atcatataaa aaataatcca ttcctttagt ggtatacgcc tctactgttt
    16141 ctttgtttac aacaatatgt cccggctgag aaattatttt ttgattgaaa ttttttttac
    16201 tagaaacagt taaagtgatt aataacaaaa cagtcataaa taaataaaat agttctcttg
    16261 tcttattgta tcttctattt ttgaaaaata tcatcgttaa tatcagagaa gagaatatta
    16321 tttgcaagct taagatccgc caaggaaatt gtaacaattt ggctggcgta ttttgcagaa
    16381 gataccatgg caccaaacta gttgtagcta gccaagtcaa acacgtacca taataaatct
    16441 ttttttctat gtttgttaat tttttaaata aaatcagggg aatacataaa gataacagac
    16501 caactagtcc cattgagtaa gtagttaagt cgttgttcat agcttcagtc agactatcga
    16561 acacattaga tgcatgagca tataaatttg tcacgctagg tttgttgatt ttttggtaga
    16621 gcatttgttc aaacatcgga taccaaaaag cactcgtcag aaccaatgtc attcctgccg
    16681 cactaaataa ttttttaaat atagcaataa aatctgcatt cttgaaacct ttagaaacca
    16741 aacgaataaa aataaaaatg ataattaatg ctgtactcat taatgttgat agtacatggc
    16801 tgtacactag caaactcata ctcaatgcta gctttacaga cggatacttt tcttcttttc
    16861 cagagatgat ttgataagca taaaataata cagggataac aagtgttatt gcgatagctt
    16921 cggcaattgc ggaacgatag tagatgtcta ctgtccgata agcagagaaa atatagagac
    16981 aactaaataa aaatgccgct tttttttgtt ttaaaaaccg ctcgccataa tgataagcta
    17041 cgagtgttgt acacaagttc aataaccaaa cgtaaataat atagcttaca atcaaatttt
    17101 ttgtcatcca atataacatt acagctggaa aaaaagtcag aaagggataa aaataattta
    17161 caccatatcc agtataattg aatgtctcat aattaatcgg actggtaaaa atagtcgata
    17221 accccttgat tcgagataag tgaaaagaaa gatctccagt ggtataaata ctctgtgtat
    17281 ttttgaaaaa gatgaaagaa tatatactac taactataat taatccactc aatattacta
    17341 cttttttatt tattttcatt cttcttgttt atctcctttt tagtcacata tcgagaatct
    17401 ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca ccactaaaca ataagtcaga
    17461 atactgatcc ataaagccat gagagcagtt ggagtatatt ttgttgtaat ttctatctta
    17521 ttttctccag catttacatc cacattcact aagccatgtt ttttataaga gtcatttacg
    17581 actttaccgt ttacttttac ctgtgtacct aaataagcat atactggtac tgtcactttt
    17641 tgctttttgt tgcttgccac tgtgtaagta atgttattat ctgcagcttt atacttccct
    17701 ttttcccacg attctcctac ttttacttca tgtttttcta agtgaggcct ttcttttatc
    17761 gcttctgttg gggcgtaatc gtataatcct cccatatctg atgttgtata acgtgggagt
    17821 gtttcttcat tgataacaat atgaccgtgt tttgattgga taacttttgc gtaattttct
    17881 tttgctgcta tagacgtgac gagtagaaac accacagtag cgccaatgat taaccatgaa
    17941 tattttttat tcatagtctt tttcataaaa acaagcgtta tgatcattga agaaaaaaag
    18001 atttggatac ctaagatacg ccaaggaaac tggagtaagc ttatcggtgt tttttgaaac
    18061 aaaaaccatg gaaataaatt agttacgcat acccatgtag aaactacgcc aacatagatg
    18121 attttttctt ttttctccat ttctttcagt atgaataaag gaaaaatcaa cgcaaccaat
    18181 cctaataacc ccatagtgta tgttgtaata tcgttattta acgcttgaat gatactatct
    18241 gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg gactgtgtat ttcttgataa
    18301 ttcatctgct gcaacatagg ataccaaaaa tagctagtta atgcacaagt caaaaaaaca
    18361 gcctttacta aattcacgac cacaaatttt atatcgtttt ttatttgctt gcttaaaaac
    18421 aaacgaacta taatcataca agcaatcaat gcactacaca atagtgtagt taagacatga
    18481 gtatacacaa gtagacttat actgacagat aaagcgatga cttttttttc tctattgaat
    18541 atcaatccat aagcataata taaaacagct gggataatcg taagagcgat tgcttctgca
    18601 attgcggatc gataatagat atcaactgtt cgataagttg caaaagtata taaacaacta
    18661 aataaaaaag cagcatctat ctttttaaaa aacttttctc cataataaaa actaatcaga
    18721 atcgtacaaa tattta
//
LOCUS       DAPCMU010000046        18531 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPCMU010000046 DAPCMU010000000
VERSION     DAPCMU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.047"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(249..1541)
                     /locus_tag="RD744_001296"
     CDS             complement(249..1541)
                     /locus_tag="RD744_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0557305.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1538..2161)
                     /locus_tag="RD744_001297"
     CDS             complement(1538..2161)
                     /locus_tag="RD744_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557306.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2164..3000)
                     /locus_tag="RD744_001298"
     CDS             complement(2164..3000)
                     /locus_tag="RD744_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0557307.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(3017..3904)
                     /locus_tag="RD744_001299"
     CDS             complement(3017..3904)
                     /locus_tag="RD744_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557308.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3912..5147)
                     /locus_tag="RD744_001300"
                     /pseudo
     CDS             complement(3912..5147)
                     /locus_tag="RD744_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(5830..6513)
                     /locus_tag="RD744_001301"
     CDS             complement(5830..6513)
                     /locus_tag="RD744_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDU0557309.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6531..7148)
                     /locus_tag="RD744_001302"
     CDS             complement(6531..7148)
                     /locus_tag="RD744_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDU0557310.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7158..7982)
                     /locus_tag="RD744_001303"
     CDS             complement(7158..7982)
                     /locus_tag="RD744_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDU0557311.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7975..8535)
                     /locus_tag="RD744_001304"
     CDS             complement(7975..8535)
                     /locus_tag="RD744_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557312.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8709..9665
                     /locus_tag="RD744_001305"
     CDS             8709..9665
                     /locus_tag="RD744_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0557313.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9970..10926)
                     /locus_tag="RD744_001306"
     CDS             complement(9970..10926)
                     /locus_tag="RD744_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDU0557314.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11030..11632
                     /locus_tag="RD744_001307"
     CDS             11030..11632
                     /locus_tag="RD744_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0557315.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11720..13219)
                     /locus_tag="RD744_001308"
     CDS             complement(11720..13219)
                     /locus_tag="RD744_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDU0557316.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13235..13759)
                     /locus_tag="RD744_001309"
     CDS             complement(13235..13759)
                     /locus_tag="RD744_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDU0557317.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14057..14806
                     /locus_tag="RD744_001310"
     CDS             14057..14806
                     /locus_tag="RD744_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDU0557318.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14797..15582
                     /locus_tag="RD744_001311"
     CDS             14797..15582
                     /locus_tag="RD744_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDU0557319.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15551..16267
                     /locus_tag="RD744_001312"
     CDS             15551..16267
                     /locus_tag="RD744_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0557320.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(16369..17403)
                     /locus_tag="RD744_001313"
     CDS             complement(16369..17403)
                     /locus_tag="RD744_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDU0557321.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(17407..18464)
                     /locus_tag="RD744_001314"
                     /pseudo
     CDS             complement(17407..18464)
                     /locus_tag="RD744_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
BASE COUNT         5549 a         3900 c         3254 g         5828 t
ORIGIN      
        1 aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa tgcattattc
       61 ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa gcagcccaca
      121 taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa atagtgttca
      181 aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta atttccgtaa
      241 atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt tcctgaagca
      301 atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac gtgagttgct
      361 gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa taaacgcgga
      421 tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg ctcaccgaac
      481 atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt atctccacct
      541 gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct gccattgaaa
      601 gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa aatcgcacca
      661 gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc ggaaaataca
      721 ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc tcgcaattcc
      781 tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct tccatagcat
      841 cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata aaagtttaat
      901 ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac tagatcaaaa
      961 tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg atgccatctt
     1021 ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat agaaaacaca
     1081 tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg tgcataacga
     1141 taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat tatttcttga
     1201 gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc ttttttcatt
     1261 gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac ttcagccggc
     1321 cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac ttttttctgt
     1381 ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga tacatcacaa
     1441 aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata aaaaaaacgc
     1501 gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca ttctcctttt
     1561 cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca gtgagtaaaa
     1621 aatcaggctg tatacaccag aacagtccat acaaaaggca taaataccac agaaacccct
     1681 tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga ctatctgctt
     1741 tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca aaaagcaaca
     1801 gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat aaataccaat
     1861 caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac atccgtttga
     1921 ttttcccttt cctccaacat accttttttt ctgccaagta atggacagat agataataag
     1981 aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc actaatccta
     2041 aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga ctggttaaaa
     2101 aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt ggctttttca
     2161 tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat tgaagaataa
     2221 gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac tcgcgtaagt
     2281 cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt aagatctcgg
     2341 cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa actggatgat
     2401 gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa agacactata
     2461 cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc tgaccatgaa
     2521 ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa tcagcggcac
     2581 atacgtccca accaaattca gccgattgta aataataaac tgcggaatca aaatcacttg
     2641 tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc ctttgaaatc
     2701 gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta tcgtcgaaac
     2761 tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag caaaccccgc
     2821 ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca gcgaaccact
     2881 cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg gataaatcat
     2941 gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg tattattcat
     3001 ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa atcagaaagg
     3061 tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac cccatagaat
     3121 taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca tcattcggac
     3181 caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt gtctgcataa
     3241 ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa aacgtctgcc
     3301 atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc tctttgatac
     3361 ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa acaagaggga
     3421 tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat cgttccagga
     3481 aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg atagaaatac
     3541 tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt cctttgatat
     3601 tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt acagatgcaa
     3661 atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc agcatcttta
     3721 tataattatc caagccgatc catgagactg gatttcccaa acgataatcg gtgaacgaat
     3781 aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg atgaatggag
     3841 aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt ttttcttttt
     3901 tcatgccttc cttctgctgt tacttcttca taaatgactt tttcaactaa aagattgtat
     3961 tggtcttgaa tttccggatt caaagcacta tcaaagacat tcacttcgcc gttgtccata
     4021 tcggaaatca tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc tgcacgagcg
     4081 agctgttctc gaatattcga catgattggt acaccacgtt ctccttttaa aatttcattg
     4141 gcttcttcac tgttttgaaa gaaatcaata aacttagcag cttcttcttt atgggctgag
     4201 ctttttgcca tactcagcat ttgcgaagaa cgtaaaggga taccagattc tccatctgct
     4261 tttctgcgag gtggattaat caactgaatc tccatatctt caggagcccc tgacaatatt
     4321 tctgtcaact ggttacttga tagaaacacc attgccgctt ctctatccct aaccgcctga
     4381 aaagacagat ccgtcgtact tgcagctcca ccacctggtt ctggatgggc ccctttgtct
     4441 accagatttt tacgcagctg gaaaaaatct gttagatagt ctggattttc aaatcccaag
     4501 ctgttagata gatcttcccg attataaaaa ttcaatcctt tctcaacttg tggtatttga
     4561 ataatacagc cagctataaa atcttcgaat cttgaaatag catagattcc taattcttca
     4621 gtgatttgat agctgatttg ttcaaattca tcccatgtcc aatttgaagc tggttcaggg
     4681 atatttaatt gttcaaatat atttttattg taagcaatcg caaacgaatt caccccgtta
     4741 gacagtccaa ctaattgccc gtcgtaagtc gtcgttgcta aataggattc atctgcatta
     4801 gaaatatcaa ttatcccttg ctccacatat tcatctaatg gttcaatctg attgatgtat
     4861 tgaggaaatt gattgttcag ttggaaaatg tcccagatat catctgatgc tgctagtgta
     4921 cctaattttg tgtaatatcc ttcataatca taaaattcat aatcgatcgt cacatgggga
     4981 tttttttctt cataaagctc gattactttt actgttgcat cgtgtcggac ttgcgaaccc
     5041 caccaagcca tgttcagcgt gatttttttg tcatcttctt gcccacaagc cgtcaagaag
     5101 aataacataa cacttaaagc taagatgatt ctcattcttt tcttcattct ctgcacctct
     5161 acttccattg ttatttttca atatactgta tttttataca gaacatattt attaaacgat
     5221 ctttttcaag gggaataaaa accaagactt gccttatcgt tctgcctagg atgaacaact
     5281 caatactttc aagaaaaatt agatagtgct gattcttctt tcatcgctaa aaacacttct
     5341 tgcttcaatg atgggaggat aattacgcaa agaaaaaagg atatcagaca actgcccaat
     5401 atccttgaca tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta aaaaacagaa
     5461 aaataactag taattctgta tgaaattagg tacaatatac acgtcgatgc acaagcattg
     5521 actatggcaa cttgattggt cagttcccgc tgacttttca agccccgaca atttagctgc
     5581 tactaaattg tcgggtgcct tttttagttt ttattcgttt ttcattctat agaaagcgct
     5641 tcaataaatc aagacatttt tttgttagaa tgctaataat aacaaaaggt tttaactctt
     5701 cttatgtaca ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa ttctgaacaa
     5761 atagtactgg agtacaaaag acgaggaaga gccactatct gctcttcctc gtcttttgat
     5821 tctcacctgt tattcatgat tctttttctc agtttcccga tatttttcca ttttacgact
     5881 aaaaagctct ccgctacttg gtggcgtata tgtgatggca tttgctcctg cttgaatggt
     5941 ctcagcaata ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt caggatattt
     6001 tttacgaaaa tggcgtactg cttctgcagt ttctttacct gcactgatat tgataatcga
     6061 aataccagct gctaactttt catctatttc tgtatatttc gacgttaccg tactgataac
     6121 tggaatatca actactcgac aaacttcttt tactgtctct aaaggcgtag gaccgttcaa
     6181 aacgacccct aacgaacctt gcgcttctgc gaacatgctc atgtaggaag aacgagcacc
     6241 ttgtgtcaaa ccaccaccta caccggagaa aactggaatg tcagctgcct caataatgct
     6301 tttagtaatc gctggatgag gtgtaaaagg atacacagca attactgcat tcgcatttgt
     6361 attacgtatg atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt ttccaaatat
     6421 cttgatcccg cttgcttcta aaatgatttc cggtactctt acgatatctt ttctcaattc
     6481 cgtcatgatt tctggcgtaa attgttctgc catcagccac ttctcctttg ctatctccgg
     6541 tcatactgat tcaattgata cgtctcgatg acctggatga ttttttgcgc ataaccgggg
     6601 tctgtcgcat aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt ggtggccgta
     6661 atcacttttt catacagcgc aggattccaa tctactcctt ggacgaacag ttgtgtatgg
     6721 tcatccatgg actgttccca tgaatcatac actttgaagt ctccttggat agttatccat
     6781 tcttcgttaa caaattcctt tgtttccagg cttactttct tctgatcccc ataagccttg
     6841 atcccgaaaa gattattgta tttgctagcc aaggtacttt ttccccaatt tgattctaat
     6901 atagcttgtc ctaaaatgat actaggaagc actccataac catcttgtag ttcttgggca
     6961 tgcggaacaa gccgttcaat aaattgctga tgggattgcg cttcattttc ttgctgatta
     7021 tcagtaaaag gagaagacag attcttcaag gaaaaaacaa aggctaagag tatcactgct
     7081 acaccggcaa ccactgctgg ccaacggata ttcttgcgct tttgcttttt ccactttttt
     7141 ttcgccatga tcaattccta cttttcattt aattcattca gatattcttc tagcgtcaat
     7201 tggtgtttgg tgatatactc agcgttttct ttaccgacat aacgtaagtg ccaaggttca
     7261 tatgtgatct tagttatatc ttcgtgtcct tttggataac gaacgataaa tccatactgc
     7321 gcagcattat ctgcgatcca tttagctcca ggctgatctc cgtaacttgc atccaataca
     7381 gtagaaggat aactgttgta ccagttttga tcgacgacat cgactgccag acctgtgtgg
     7441 tgttcgctgt atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc ctcctcttct
     7501 gatacacctt gacgactcat cacatcttgg acattttgtt cgaaaacttg ttgttgggat
     7561 gatacagacc ggtaagctga gaccatcact aatggaaatc cagctgcttc agcagccttg
     7621 gcaaatgctt catattctgg agcaatgcgt ttgtcgatca agtatccgtt acttaatgat
     7681 gccaattgag tagactcatc aatttctttt tctaatttgt gatctggacc aaccaaaact
     7741 agcttccaat cgtctgtttt aacatctggc aattgtttgg acgtacgatc ttttgtttct
     7801 ttgtccttta ccttgctgct ggttgtttga ctgttctttt ctttactagc agttgttttg
     7861 gtgcttgttc gttcgactgc ttgattgccg gcttgaacat gcgaatcatc atcgctaaaa
     7921 ataacatagc ccattgctgc gatcataatc acaactgctc caaatcctaa aaatttattc
     7981 acttgaaaaa tcctttcgtt gttcttcacg ttccccattc gcctcatgaa cgtaagtttc
     8041 ttccagattt tcattcaccc aatcgagaag ttcgattttt tctccttcga tctgtttttg
     8101 aacatctgct ggcagacgaa aattcaaaat atcatcatag ccccattcat catattgaat
     8161 acttactttc aagtcaaggt atctaccagc ctgctgatta tttttcaaag ggatcagctc
     8221 gacttttgct gctttgctgc taagccattt ttgcgcgatg cgtgttttga tttgcttgta
     8281 ctctgcaacg gcaacgtttc gtttttcggg aaaaataatc gtttgacgca aaatcttttg
     8341 cgttagcatc cattcataat cagtgaaaag attttttact ttctgcatcg cttcagatgg
     8401 cagatttttc tcgccatctt tccatttttg ccattgttct ggactgatca gcaagtattc
     8461 atctaaaaaa tttttttcag tttcaaactg ttctaataat acactcataa taagatcaat
     8521 atatacttga tgcacgttta tcccctcctt ttaactattc tacttaagtt ttgatcaaaa
     8581 agcacctctt aaatcgctgc tttcattttt tttaatgttt caagtcctct cctctttatt
     8641 cttcgtgatt tttgttataa taatagacag acaagaaact caatccaact atgaaggagc
     8701 gttttcagat gaaaaaatat caatggggaa tcgttggtct tggtaccatc gcacatgagt
     8761 ttgccgaaag cttcaatcaa gagacgagcg aactaacagc agttgcttcc agaacttcag
     8821 aaaaagcgga aaacttcgct caccgttaca atattccaaa agcttacgga agctatcaag
     8881 aaatgttaga cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat agacagcata
     8941 tcgaccatat cttggcagca ttggaagcag gcaaacacgt tctatgtgaa aaagccatca
     9001 cgatgaataa aaaagaactg gctgacgcta tgcgcttagc agaagaaaag aatttgatct
     9061 tagcagaagc gatgacaatc ttcaacatgc cactttatca gcagttacgc tcaataatgg
     9121 atacaggaaa actgggggct ttaaaaatga tccaagcacc tttcggcagt tacaaagaac
     9181 cagaccctaa aaaccggttc ttcaatccag aattagctgg aggagctctg ttggatatcg
     9241 gtacgtatgc tgtatctttc gctcgtttct ttttgagctc tcaacctgaa gtagttgcct
     9301 ctacaatggt tccttttgaa acaggcgtgg acgaacaatc cgtcactatc ttgcgtaaca
     9361 aagaaaatga acttgctgct gtcagcttga cttttcaggc aaaaatgcca aaagtcggta
     9421 tcgttgcctt tgagaacggt tatgtgacga ttgctgatta cccgcgtgct gatcgtgcgg
     9481 agatcttatt tacagatggt acaaaggaat tcatcgaaag cgggaataca tctgaagcaa
     9541 tgaattacga aataaagaat atggtacaca tgattgaagg tgacttgccc aatcgctctc
     9601 tcttcttgac aaaagatgtc atcgaaatct tggatcaaat gcaagttcta tggaaaaata
     9661 tgtaatttta aaaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagccaaaca
     9721 aaaatagctc ctcctatttt tcgtttggct ggacttatta ccgcagagga aagcttttga
     9781 acaccgttta agaaagaggt tgtgagccta ccgttcagct ccgagtcaca aggagcaatt
     9841 tcaaaaaaca gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttgacagg
     9901 agaacaccgt ttaaagaaga ggttgtgaaa tcagcttttt gacctgaata ttagctgaat
     9961 tcacaacctc tatttttgga ttttttgttg ggagaattct gccttgattt caataaaact
    10021 tttaaaagac tgcaaaaact ggaacaattc tgctcgatcc tcgaattcag ggcgactagt
    10081 cggcagaggc atttgccgat acaatacata aacttcttca atacgtgtta aaatttgtct
    10141 gccatcattt gactctgaaa atgtttctgc agtataaatc aataaaccat agatatgttt
    10201 tccataataa gctggttgct ggattctctc tagattttcc agcatgtctt taatcacatt
    10261 ggcctgtgct cttctcatag agaagtaagt ttcgtagtat agcggctgtc tgagccagta
    10321 gttttcttga tgttctctgg catcttcttg gctttcgcga atgaatgtca gtaaatgttc
    10381 gcatttgatc tgcacatctt ctttattttc tgataaaaga aattctgcca tttgtctcaa
    10441 aatatttcga aattcttttt cgatctgtat ttggttttcc ttcagctgct tcgtacgatc
    10501 tggcatgtaa acatttgcaa gcaaagcaaa tactaatcca atcgccatta atcccgcttc
    10561 attgccgatc agctgccaag acatgttttc ctccactaaa taatgagtga ccaaaaccga
    10621 atttactact atcccttcgg ttaatttgaa gcgagaggca agcgggatga acaggaataa
    10681 aaaaagactg aagccaaaaa tagtataccc aacagcaaaa aaactgaaca gtgctgttgc
    10741 cgtagctaat ataaaggctg cgattcgatt catgcccgta cgcaatgtag agcgcttggt
    10801 atttcctaca cttaataatg caatgatccc tgcagctggc caatacaaca gcgataagct
    10861 acccgctgtg accatcgaaa agaagacact gatcactgtt ttgactgttc gcattcctat
    10921 acgcatttac ttcccccttt tttattttaa ttatacttga ataatgaaaa actagctatc
    10981 agacaaaagt ttaggtatga tagtaaaaag accaaaaagg agaatctcta tgcaatttgg
    11041 ccgttttcga ctaggtatgc gtacgatcaa aagtgcctta gctgtttttt tatgtatctt
    11101 gttttttcat ttgactcaac gaggaatccc gatgattgct gcattatctg ctgttttttc
    11161 tttgcgtcag gacttgacta caaccgtttc ttttggaaag tctaggatca tcggcaatac
    11221 attaggtggt acattagcta tcatttattt ttttgtcaaa agctattttc acaatgattt
    11281 tttggtagaa ttgatcttgc ttcctattct agttattatt gtgatcgtcg tgtcagatgg
    11341 tattaacaat aattccggta tcatttccgc aatagctacg ttgttgctga ttgcgttgag
    11401 catccctcaa ggcgaatccg ctttgtatgc tatccaacgt gtactagata cttttatagg
    11461 gacattcatt gcaatcggta tcaatttctt tcttcgtcct cctgaaacag aaaagaagga
    11521 agaactatca gaagatttgg tcgaactcca aaagaaagaa cgattgctaa gaacaaattt
    11581 acaagagata gaagctaaga tccaaaaaca aaagaaaaat agcgaggact aattttcttt
    11641 tgcaataaaa taaaaaaatg gagagaagaa ctttaaaaag tcttcctcca tttttgattg
    11701 aacggtgaag tgtactactt tattcttcct tcatcgagtt ttgttctgcc tcttttggcg
    11761 cttcatacat atttacttgg ctagtagtcc cacgtgtcga caaattctct acgatttcct
    11821 tcaagtcaag tcctgttgtc gcttttagtg tttcttgagt agaagaaagc aaatttgtgg
    11881 cataatttgt cactcggttc gctccactgg tttctcctga actgcttcct gtatcgacaa
    11941 cagagatttt ttcgatattt cctagcggct gtgctgcttc gcgcatcaat tgcggtagca
    12001 tatcgataac catactcaag acagctgctt cgccatattc cttgaaggca ttggcgatct
    12061 tttgttttgc ttcggcttct gcttttcctt ttgctagtgc tgcttcggct tctgcttgtc
    12121 cagctaaacg tgtcttattg gcttccgctt ctgccagtgc ttccacacgg aatcgttctg
    12181 cttcggcttc agtcacttct ttgacttttt gagcttgtgc ttcttgttct ttagcgtacc
    12241 gatcggcatc tgcttttttc ttcacttccg aatcatattg cttttcacga cgtgtgattt
    12301 ctttttcttc cagttcgatt tgtttttggc gttctacgat tttaatctgc atttcctgtt
    12361 cgattacttg ttgttgtgct cgggcacttt ctaaattgta ggcttggtct gcttttgctt
    12421 tagcaacatc ttgttcttgt ttgtaggttg ctaatttcag ttctttttct tttaatgatt
    12481 ctgcgatctc tgtttgtcgc tgtaattcag ctgcttgtga ttctttttct gcttgagctt
    12541 ttttgatacg cgtttctttt agagcttccg cttctgctat atctgcatct cttttgactt
    12601 gggcgattct tggtttccct aaagagtcaa gataaccgtt tttgtctctg acctctttga
    12661 tagtgaaaga aacgatgatc aaccccattt ttgcaaggtc tacagaagcg acttcttgta
    12721 cgctttgact gaacttatcg cgattttgat agatttcttc tacagtcata gaaccaagaa
    12781 tcgagcgcaa gtgtccttct aatacttctc ttgcttcatt ttccagttct tctcttgtct
    12841 tgcctaaaaa ctgttctgct gctgtagcaa tctcttctac agatgaaccg attttgatga
    12901 tcgaagtacc atcacacatc accggtacgc cttgttcggt atacacttca ggggtggaga
    12961 cgtccaattt actcgataat agactgatcc gattcgaacg ttgaaaaact ggcaagacga
    13021 atgcgccacc tccacgcaca attttgattt tgttattgct ttcatcaatg tgcacatttt
    13081 tgcttcctaa ataacttcca ctgatgatca acgcttcatc cggttttgct gtctggtact
    13141 tcgctacaaa gacgatcagc aacatcagta aaatcaaagc tgcgattgcg atcgcgataa
    13201 aaacaactgg tatttccatt taacacacgc tcctttaagc ttttatcatg ctttcataag
    13261 gcatgacata acagattctt tccttgatct caatgatcaa tactttcgtt ccccgctcag
    13321 ctgtctgttc ttgggggctg taaaaacaag ctggacgact aatggaaccg gtaacacttt
    13381 tcaactgaat ttctcccata cctcgtacag ggatcggtgt gatcacaaca gctacttgtc
    13441 cttccagagt tttttcagaa gacgacatcg ttgattcggc atgtttcatc ggcatcaata
    13501 tatagaaatt aagcaaaaat accaacacta tcgatatgcc tccactagca ataaataaaa
    13561 ataaagatga ttgatctgtt aaacgctcac ctagaaaacc aaataagctt gtgaaagtta
    13621 accaagggat aataagcatc ggatcaagcg ggccatcaaa atcgaataca tctccaaaaa
    13681 taatcaatag gactgccaat cctgcacaaa tcagcaatgt ataccaataa atcgtctcaa
    13741 gtgcgattcc ccctatcata tacacgcctc cttccatttc tacgtctatc ataccaatgt
    13801 ttgaaaattc tgtatagctt cttttaccga ctcagtggtg ttttttatag gaaggttgtg
    13861 tttatcgttg acaatttgtt aaatttttgt tttcatgtta aaattttcac aataaattct
    13921 ttcgaggtag cctatgtttc ctttttttaa gaaaaaacca ttaaagctcc agaacacgat
    13981 catccttgtc gtttgtttct ccacattcgt ttctttgatt gtttctgcca tattgatcag
    14041 gaattttatt ctttcgatga accgatctag tgtgtcagat aaaatctcta gcattgctaa
    14101 aatcgttgcc aatgataaac tcatccaaaa aaagctgcag actggtgacc cgacacatgc
    14161 gattcaagga ataacaaatg aaatcatgat tgcatcgaat ttagatttta tcgttgtcat
    14221 ggaccctgaa ggcattcgat attctcacgc agacccaagt tacatcggtc ttcctttctc
    14281 aagtcatgaa gatgtgaaaa aagtgattgg aggacaagaa tttttctctg aaaaagaagg
    14341 aatgttaggg ttcggcttcc gctatttcgt ccctatttat tatgatcaag agattattgg
    14401 tgcagtttgt gttggcttaa caaacaacac gattgacaat caagtcaaag atggacaaaa
    14461 aatgattgtt ttttcattga ttattggact tagcgtaggc atcataggcg ccgtttttct
    14521 tgcccaaaaa atgaaaaaag tactgcttgg acttgaacca caagaaatag cagaacagtt
    14581 aagtgaaaaa acgattatcg aaaatgaagt agatgaagga atcttagcca tctcttctga
    14641 taaaactatc ctcttgatca atacacctgc aaaaaacatt ttctaccaaa cagagcagct
    14701 attggatttc gaagtaggaa aaaaatcagt gataatctat atgacgtttt gtttaaagag
    14761 acgattgagc atcaaacgac cgttcggact caatccatgt atttaaatgg gatagaaact
    14821 atttctagcg ttacacctct ttttagcaat gaccgttttt taggagcagt cgcaacttta
    14881 cgaaatcgat cagaagtaat gaaattaatg gaggagcttg gggggacgaa acaatatata
    14941 aactccatga gagctcaaac ccatgaattc atgaataaac ttcatgtcat ttctggtttg
    15001 attgatttga agaagtatga agaagtcacc cattttatcc agcagctaaa tcaaaattat
    15061 aaaaaagaga ttggccaagt ctcaagtctg atcaaagtac ctgcaattgc cggtttttta
    15121 ttaggcaaaa taaacgaagc caaagaacaa tctgttgaaa tgctgttaga aagcgattct
    15181 tttatcccta aaatggagat ggatgactcc atccattcac ttttgcaagt gttagggaac
    15241 ttacttgata atgcaaaaga agccgtgcta caaaaacatg aaagaaacgg acaggtccgg
    15301 attcatttaa actatgacac agaagctgaa gtattcatcg tgaccgtctg cgatggcggt
    15361 gtcgggatat cagaagaaat ctcagaaaaa atatttacgc taggtttttc gacgaaggga
    15421 atcaatcgtg ggtttggatt gaatctcatc cattcaatcg tcaaacgcca cggtggattt
    15481 ttagaatttt ccaaacaaac aaatggcggt acaatcgtct atctagaatt accaaaaaag
    15541 gaagatcaaa atgatgacaa atgtcctgat aatcgaagat gatccaatgg ttgcagaatt
    15601 gaatcgtact tttgtggaaa gcatccctaa tatgaacgta atcgaaagtt tcagaaatgt
    15661 agaagaagga aaaaaatatt tcagacaaaa aaacgcagat ctgctgctgc ttgatgtata
    15721 catgcctggc gaaagtggta tccatttttt atcttggctt agacagcaag gaaatgatat
    15781 cccagtgatt ttgatcactg ctgcagatga tatcgaaacc ataaagaatg tgatccgcta
    15841 cggtgcctta gattatttga tcaaaccgtt cactttgcaa cgttttcagc tggcgatcga
    15901 aaagtatctt gctttctctc agacaatcca aacgactgaa aaagccgatc agaaattgat
    15961 cgatcaagta ttcaaccaca ccactcccat tcacgaaata ccatcattca tcgatttgcc
    16021 taaaggtctg tcaaaactga ctttaaaaaa aataagtgca tttattctaa aagaagatac
    16081 tattttttca acaaaagaac tggctcaaaa aagctcactg tcacgtatat ccgtcaagaa
    16141 atatttgaat tttttagtag aacaagatat attgaacgaa agaatcaaat atttggaaat
    16201 cggccagccg ctaacctgtt atgaattgac aaagaatggg cggaaaaagc tcacagatta
    16261 ctgttgagtc atcgaattat cccatacaaa atagtccaag tatacagaag taacaactgt
    16321 ttacttggac tatttttaaa cattttattg acgaatatcc atgttttctt atcttaattg
    16381 acgggcttta ggaagagata cctcattttt cgtttttcca gtttctatta tttcacgaaa
    16441 gttctcgaaa cttgtcgaaa tcatatttga taatgcctca tctgtatttg aacctacatg
    16501 cggggttaca agaactctag ggtacatttc tactaattgc tggatagcag ggtctggaat
    16561 cgtctcccat ggcttgaagg atcggaaaaa aacctctgtt tcatttgcaa aaacatcggt
    16621 tgcaaaacca gctaaatgat tgcttttcaa agctcttaaa atagcttcat tatcttgaag
    16681 ctctccacgg gctgtattaa tcaagatgct accttttttc atttgagcta aaaatccttc
    16741 attgatcatt tgatcgtttt cacctgggat atgaggtaca tgaatactga caatatcgct
    16801 tttggctaat aactcttcaa gcgagtggaa cgtcaatact tttttagcag cttctgtctg
    16861 atgaatatcg tagccgatca ctttggctcc tagtccctta aacaaaacag cttccgtcaa
    16921 ccctatcctg cctgttccta tgatccctac tgtgcaattt ctgatttcac gactaaacat
    16981 ttgctcatcc actcgaaaat ctttgaaaga tgttcggatc gtcgtatacg cagtatttct
    17041 caaaagcatc atggcaaaag tcaaagataa ctctgctatc gcatttggcg aatacgatgg
    17101 tacacgggct actgtcatac caaaatcagc tgccgcttgt agatcgatat gattgactcc
    17161 cacagtccgt gtaaaaacat aacgaatccc atattcctgc atttttgcaa gattggtctc
    17221 atctgctata caatttcccc tcaacaatac agcatcatgg tcctttgctg acacgatatt
    17281 gtcatgtgtc aacagttctt cataaagact gagatcataa tggtaaatat tcaatttttc
    17341 aaaaaaatct atctcgttcg gacgaacacc gtaacatgcg attctcatac tttcttcctc
    17401 catttcttat gaaaataaac ctatgccatg aatcatttgt aatacaacga tccatacagg
    17461 tgtcagtacc acagcaaaaa tagttgacag taaggatgca ttggaggcta aaagtgcctc
    17521 cctatcaaaa ctgatagcat aggctgctgc cactgttgca gctggtgtag ccatcatgat
    17581 aacgattgtt gccagtgctt caaaattgac tggtagaata ttcgttgccg tcaaaactgc
    17641 tagaatcacg atattcaatg caggtacaaa taccactttg actagactgt aataccatga
    17701 agttttttca gatgcagctt ttttgaagct tacttcacct aatgttgcgc cgattgctag
    17761 ccacgccaat ggtgaactta aaccagctaa ataatccatc ggctgaaata gccacggtgc
    17821 agtctggtca attcgcaaaa acgctacttg gcttatctct ccttctgcac ctgtaactga
    17881 cacttgtggc agataccctt ggaacagcca aatgaaaagg ccggcaaaag tagcgataat
    17941 gattggattg acgaacatcg ctttgatatt tttagcttcc atcttcaagc cactcatttt
    18001 tatgtatcca taagaataca aaaagattcg gtagccaata ttgaaaattg aggaatacat
    18061 cacgccgaca ggaccataaa tcgcactgac gataggcgtt ccgaaaaagg tcgtagaacc
    18121 aaagatcgtc aggacacgca acgttgtttg cttatcccct ttatagctta agaaaaaagg
    18181 ttttgaaact agaataagaa taatataaat aactaatccc caaatcaaga cattgattcc
    18241 ttgcatcagt aattctgtat tgatatcttg catgaaagct cgaaatgcca gtgctggcaa
    18301 agccactgtt aagacgactt tcgatagaat ttttccaaca gattcagaaa aaatcccctt
    18361 tttctacaaa caaaccctaa taatataata aaaactgttg aagtgattgc actgacaatc
    18421 gacatatctg tcaaagtcga tcgaaaaatg tcaaataagt tcatctttct cctcctaatt
    18481 atgttcattt attcacaata aaaaaagctt ttattttgct aatacgtatt a
//
LOCUS       DAPCMU010000047        17951 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPCMU010000047 DAPCMU010000000
VERSION     DAPCMU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.048"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="RD744_001315"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="RD744_001315"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDU0557322.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="RD744_001316"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="RD744_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDU0557323.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="RD744_001317"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="RD744_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDU0557324.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="RD744_001318"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="RD744_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDU0557325.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="RD744_001319"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="RD744_001319"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDU0557326.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="RD744_001320"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="RD744_001320"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDU0557327.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="RD744_001321"
     CDS             complement(8053..8523)
                     /locus_tag="RD744_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDU0557328.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="RD744_001322"
     CDS             complement(8569..8994)
                     /locus_tag="RD744_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDU0557329.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="RD744_001323"
     CDS             complement(9061..9330)
                     /locus_tag="RD744_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDU0557330.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="RD744_001324"
     CDS             complement(9341..10936)
                     /locus_tag="RD744_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0557331.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="RD744_001325"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="RD744_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDU0557332.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="RD744_001326"
     CDS             complement(14489..15049)
                     /locus_tag="RD744_001326"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDU0557333.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="RD744_001327"
     CDS             15402..16376
                     /locus_tag="RD744_001327"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDU0557334.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="RD744_001328"
     CDS             16546..17946
                     /locus_tag="RD744_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDU0557335.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4875 a         3974 c         3238 g         5864 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa a
//
LOCUS       DAPCMU010000048        17680 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPCMU010000048 DAPCMU010000000
VERSION     DAPCMU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.049"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..1174)
                     /locus_tag="RD744_001329"
     CDS             complement(<1..1174)
                     /locus_tag="RD744_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDU0557336.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQ"
     gene            complement(1198..1929)
                     /locus_tag="RD744_001330"
     CDS             complement(1198..1929)
                     /locus_tag="RD744_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0557337.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(1947..2342)
                     /locus_tag="RD744_001331"
     CDS             complement(1947..2342)
                     /locus_tag="RD744_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDU0557338.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(2444..4081)
                     /locus_tag="RD744_001332"
     CDS             complement(2444..4081)
                     /locus_tag="RD744_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDU0557339.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(4116..5450)
                     /gene="gmhB"
                     /locus_tag="RD744_001333"
     CDS             complement(4116..5450)
                     /gene="gmhB"
                     /locus_tag="RD744_001333"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDU0557340.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(5492..6601)
                     /locus_tag="RD744_001334"
     CDS             complement(5492..6601)
                     /locus_tag="RD744_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557341.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(6626..7861)
                     /locus_tag="RD744_001335"
     CDS             complement(6626..7861)
                     /locus_tag="RD744_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557342.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(7892..9022)
                     /locus_tag="RD744_001336"
     CDS             complement(7892..9022)
                     /locus_tag="RD744_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0557343.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(9009..9782)
                     /locus_tag="RD744_001337"
     CDS             complement(9009..9782)
                     /locus_tag="RD744_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDU0557344.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(9796..10251)
                     /locus_tag="RD744_001338"
     CDS             complement(9796..10251)
                     /locus_tag="RD744_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HDU0557345.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(10450..10683)
                     /locus_tag="RD744_001339"
     CDS             complement(10450..10683)
                     /locus_tag="RD744_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557346.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            complement(10673..11779)
                     /locus_tag="RD744_001340"
     CDS             complement(10673..11779)
                     /locus_tag="RD744_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0557347.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(11797..12558)
                     /locus_tag="RD744_001341"
     CDS             complement(11797..12558)
                     /locus_tag="RD744_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HDU0557348.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(12634..13632)
                     /locus_tag="RD744_001342"
     CDS             complement(12634..13632)
                     /locus_tag="RD744_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDU0557349.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(13651..14304)
                     /locus_tag="RD744_001343"
     CDS             complement(13651..14304)
                     /locus_tag="RD744_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0557350.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(14314..15324)
                     /locus_tag="RD744_001344"
     CDS             complement(14314..15324)
                     /locus_tag="RD744_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDU0557351.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(15343..16593)
                     /locus_tag="RD744_001345"
     CDS             complement(15343..16593)
                     /locus_tag="RD744_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDU0557352.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
BASE COUNT         5602 a         3892 c         2483 g         5703 t
ORIGIN      
        1 cctgataccc atataacttt ataaaaacat aatttagtac aatattcata attgcacaca
       61 aaaccgatgg aataacgaca gtgagtttct tttcaaagta ttcctgtact ctagcaaaca
      121 gctgaaacat atagttgaaa taaataccga tacatatagg tacaattaaa ttaacactgt
      181 cgatatattt ctgcccacca aatatcaaca caatttcttt actaaatagc attaatgcaa
      241 aaagcatagc cgctaccaaa ccaagaactt cattagctct ttttgatagt aaatcatatt
      301 ctcctgactt gattttttga tactgataag gtgcaaaagt ggaattgaca gaatttgtaa
      361 caaggttaat tagagcacca atagaatatg ccacgctgta aatagcaaca tctgacgaac
      421 cacataagta atttatcata attttatcag aaccattcag aataaattca gataaatagt
      481 gtggcatcaa cgatacacaa aatactactg cgaaattcca cacttcatta tccttaaaat
      541 ccttacatct tctaaatacc gatatataca cagccacgcc aacaacacca gtcactagga
      601 cattcattaa ggctctcgcc tcagcggtag gagatataaa cagaaccgct aaacagccaa
      661 ctacagatga gccaacagta actgcaaggg tagctactaa taaagtctta taatcatata
      721 tatatcgttt gtgtaaagac caacattgta tttctgcctg aaatataaaa tatgagaaca
      781 tacagacaac tagaactggt gtcaatccta aaatttctgc caatatatcc ttaataaaaa
      841 tagcaataac aaaccaaaaa atagtcagta ctaaagtcaa tccggatact gtcgaaagaa
      901 tatgttcttg attatcatat tttacataaa gattcatcat ggctttgaac aaactaagcg
      961 atgtaatcat gagcaataag gattcccacg atagataagt gctgtataaa ccgtattgtt
     1021 ctgtagacaa aactctagta aaaattgggg ttgtaatcat tgaaacccct ctctgtaaaa
     1081 aaccacatac cataaaccat aatgcagcct taacaggaag tggagtattc ttatatctgc
     1141 ttaatgtttt tacaattaga ttattactgg tcactgctat tttctcctaa acactattta
     1201 ttcttgattc aaaaacacat tgagctcttc tggagtacca agcacatgaa cctttttcgc
     1261 atctactatg ctaatagtca cctttaaccc tttttttatc ataaagttat ataatggtgc
     1321 tatatatttt tcattcttct ccatattaga ggagtctgca taatactctt catataaagc
     1381 tttatacagc tttgccgatg agaaataata cgcaccaaga gtacaattat cagaaatgcg
     1441 aactttttcg cggacttcta ttacttcatt tcgatcgtta agctttgcaa aactccaatg
     1501 atcgccatca gcatgaaagc atggaatatg tccatctcca caaatatctt cgtatttcat
     1561 ctcatttgat tccacgtatg tatcaatatt gtaaaccata atctcattgt cttgattgca
     1621 ataaggaata gccaacatac atgtagttgc ttgaccatct gtcatatagt ctatttcaat
     1681 cacttctacg ttttcaatac caaagtaagc acaatgctcc tttataaaag ctgaagcgtt
     1741 gtcttcctga cgaacaacaa aaatatactt atcaacattt ttattgtatc caaccaaact
     1801 atccatggcc caatcaaata aagttcttcc tttggcatta atcatatatt taggaaccgc
     1861 ataaccaatt tttcgaaaac gacttcccaa acctgccatc gtaatgataa ctgtcatctt
     1921 tttttccata gaactaacct ccttatttag aatctttttt ttgaactgtt gcagatttca
     1981 tttaattcat ctacagagca tctcaaaaac tcatcaggtc taaccgttcg atcatctaca
     2041 tagaagcctt tatgccccgg ccaaggctta ccatacagga tttcatcata cggaatgtcc
     2101 catttatcca accacgccaa gagcactttt gcagtattag catttatcat tcccaaattt
     2161 cctttatagg aattaacatt gcgtgaggtg aacaatgtaa ttttagcacc attctcctta
     2221 tagaatctca gtttttcaac catattctca tacggcacca aatcctcata tcgctcatcc
     2281 ttcttcttta tcggacacaa agtgccatcg atatcaaaca caaaagaata ttcactatac
     2341 atttttctct tcccttctat tgtattaatt aatataatcg tgccttaatg cctacattgc
     2401 taaaacagta ttgtaaccgt tattcattaa ggcacaattt atattagagt ataactttat
     2461 tacttactct tgcaagaagt tccatacctg tagcaagcat cactatttgg tgatcaaaac
     2521 tttcaccgtg taatggaatc atactaagaa atagcaacgc ttctatcaat acaatcttat
     2581 caaaatcgtc cttaatctcg tccgaaaaga ccgacttaaa caattcaaat aaatcgtaat
     2641 ctcttacacg cgtattaatt gtataatcta tcactgcaca ctcggtatca tattctacag
     2701 taaatagatc cttgatgata taatcatatt taccatctaa actatgaaat agttttgcta
     2761 attcatatct gctgtcccca taaatatcat attttccaaa ttttccccta ggatcaataa
     2821 ccttaacaaa ggaacaattg gaatctacca tcatattagc aaaacacaaa tctccatgta
     2881 taatacaaaa ttcatttaca tcatagagca tacttgggat aagattcttt agaagttcaa
     2941 ttacaccggt taaagaaata aacttacgac cattaatttg aatttgtttc tcaaaaaaag
     3001 ctgaaaatct tttatcttca gacaattttt gaaatctctg catggttttt cgcaaataca
     3061 tatcttccaa tgactctcta atcttttcat cttttaaggt gtatcgctta aaatcgctac
     3121 agataaactt tatacggcta aacagatcat gccactggtt atattttaaa tcaccatata
     3181 agtataactc atgaaccgta tgatatgcat aatattccat atcaacatag ggagctacgt
     3241 aagatgtaga ataatcaaaa attctaggtc ttacatattc aatatcagca ggaagtttta
     3301 aataccactt tatttctcca ataaatttat ccttgtcatc gctagttttc ttcaatatcc
     3361 ctcgatcttt atctatagag atatgattaa actctcgtgc tttaacttcc aactttgtat
     3421 tgtaatattt atcgatgtgt ccaatatcaa accacttttc tgttcttacg gcctttagcg
     3481 gacaacgttc gctgtatagc tgtaaagccc gataaaatga gtttattccc ccctgtttcc
     3541 gactctcaac tgcctctctt aagcaactct tcagaagttt tgacgaagaa aacataaata
     3601 cgccaataaa taatttttca cgtttatcgg atacgtaagc ttcctcttta tcgtggatat
     3661 cagtaattat tccttcatta attttaaagt atgtccaagt actcgacaca aaatcttcag
     3721 aataatagaa tgtatccatt tctatatcag acatactctc cataacaatt gtatctgcga
     3781 agttaattag aacaggctca tcttcatctg ataacccaaa atacacggtt tgccctagat
     3841 cccctaattc aggaaggtca catatatgaa ttctggaatc atgatagttt ttcagtcttc
     3901 tatgaacctt atccgctttt tcataacaga taatatccaa tgaatcgcag attgcattat
     3961 actgattata caaataatca aagacaattt ttttattaat tggatagata attgctggta
     4021 acattcctat actctgcaat tctttttcaa caacttttgc tgctggaatt aataccttca
     4081 tctaattact ccttttacaa gtttcactaa accaattatt gcatagtgca taaatgattt
     4141 ttaacaaatt ctaacaatga acagacacta tagtcttggc cataattagc aggcaaggtg
     4201 atgtcttctg taatcagtac tgtcttacaa ccagcatttt ttccagcaat tacatcactt
     4261 tcactatctc caaccataaa tgattgactt aggtttatgt taaaatcctt tgatgcttct
     4321 aataacagtc caggttttgg cttgcgacaa ttgcaatcaa tttttaattc tataacctct
     4381 cctgcatatc ctttatgggg atggtgcgga cagtaataaa taccgtttaa aaatccacca
     4441 gcatgcccta gcattgtttc aagcttattg tgaatactat taagctcatc aacagtaact
     4501 tcacctcttg caattacagg ctgatttgtt ataacaatac acagaaaacc tagagaatta
     4561 ataattttaa ttgcctccga tactccatca attaactcaa attcttctgc atttctcaaa
     4621 aaaccaacgt atttattaat cgttccatct ctatctaaaa agattgcttt ttgtggattg
     4681 gatagatttt taccaacaat aattcccttt tgaaagtcct taattacagc tctatatcgg
     4741 tccggtgttc ccatatcttt aacatactct ggagaatcgt agcaatacat acgccctgtg
     4801 ttagccaatg gtttcagaat cattctatct aaatcaattt tgtatggtag ccccccacct
     4861 tcaactattg atttatttac aatttccatt aatggatttt ctctcaacac cttcggattt
     4921 ataacatgaa gacctgcatt tactcggttt ttataatatt caggtctttg atcttccttc
     4981 gctaaccatt gaagcacttt cttattgtca tcggatataa ttaatccact atcatacgga
     5041 tgtgagttag gatgcgtaaa caaagtaaca tctgcgttat tctcaacatg aaatctaatc
     5101 atctgcctga aatcaacatc aaatactgca tcagcattca aaagtaaaaa cggttcatca
     5161 cccagttcat ccaacaaaaa atacaatgca cctgcattgc caagtggagt tttttcaata
     5221 aaatatttga ttttaattcc taattttgaa ccatctccga aatgctcctc tattttttca
     5281 tgcaaatagg aaacagtcaa aataatatca ttaaagcctt gatctctaag cgaacacaat
     5341 tctcgttcta aaactggaat gccatctata ggaatcattg gcttaggaat atccggaaac
     5401 aattccgaaa ttcggcttcc tcgtccgccc gccataatta ctgttttcat aatatgccct
     5461 tccctaaagt actttttact ataaaagtta attactcttc ccaaacaaag tccatatatt
     5521 tttcatattc tgctttgtat tcgtattttc cagtgtacaa attcaaccaa tttataaaat
     5581 caaaattata gcagtgaagt gcatcttggc tatcatcaat ataatacgta ccataaatag
     5641 tattatttga ataacgacct acatatttat gccagtacaa atcaatttga tttttgttaa
     5701 tagtaataac atggtttttt acaaactccc aatatgccct aatagagcac tcctcattgc
     5761 cccccattcg tgaaatatag cttcgaagaa gctgaacttc tggagctaaa ttattttcag
     5821 atatctccct tcttgagact cctctagaca cagctccctt ttctctcaca tcactaggaa
     5881 tcgagaagag gtttatcata tcatcaacat caccaaaata caggaagtct gacaaacaat
     5941 acgggaaaaa catgtctcca tacggcattg atagtaacag taatcgctga ttttgtatta
     6001 atttccaatc atcttcgtta ttaacaggat actgttcaag taaactgcag aaaaacgcca
     6061 ttgcatttgg atggtatatt ctttgatctg ttctagtttt acaaacatac tttgcaccaa
     6121 gctgctttgc tctgactatt ccaggcaaag tgtttccaac ttgataatta atatttaagt
     6181 gtccaggctt ttcaggtttc tcactacaaa taactataac tccaagatcc tctatccttt
     6241 tcaatgcctt aatatcttcg tccttccatg tcgaaagaat aattaaagca tttggataac
     6301 atttcttata gtaggccaga gtattataag agaaatcatc ctttaaacat agtggcccct
     6361 gcattacaat tgctacatct ttgcaacaat taactaaacc catcactgga acatcagaca
     6421 tgtcctttga gcgcaattga aacgctgcat aaacttcaga attttcttct actgttgcac
     6481 aatcagcagt caatttccaa gatacgagtt tacttatatt atgaccaaac atgttcctaa
     6541 acacatatgg taaattttcg tacttcttca atatgttata taaaatctct ttcttattca
     6601 tgagatgcac tcctattaat atttcctatt ctatgttagc cacacttcta cattcttgat
     6661 agatgtattc tataaccgct aagcatacag gaatagctgg agtaaatatc ggataaaacc
     6721 agtctatatt aatccctgtt agcaagacat ttaatgtaat tccaaatgct aaatattgat
     6781 agccagtcat acccactttt gatgcaagta atgctgtttt gaaccctttt ataataaaca
     6841 gaatatcaaa tataagtata cctacaccat attgagtaat tgtatagaaa aatccaatat
     6901 cagataggtg tcctctaact cctcctggta atataaacgt aaattcctca ccagtaagaa
     6961 taccagtacc gaacaaaggc gattgcaaaa atttaggaat aaaaaaccaa actgaagcaa
     7021 atcgagattc agcagatcct gcatattcac ctgtactgtt tgtgaaagtt tcaacaaatg
     7081 ttgagaacat ggtcgaatta ataaatatga ctacaagcaa cccagctaaa acccacattg
     7141 caatttttct tttcgaagaa cctttcttaa aaaggatata gcaaccaagt acaataattt
     7201 gatagacaag gacagatcta gaaccatgaa taacagcaga atatgccagt gcgctagcaa
     7261 tcagtaaata atattttact tttcttctag ctacaacttg tcgctcaatc atatacaaag
     7321 caatcggtat tattataatt gtaaaacatg gtggattaat tcttaaaaag cctccacgat
     7381 accaattttc cggggcacac tcacgatata tgttttcata gagaagtact cctgaaaatt
     7441 tctgaataag tgaaatagat gttctaagaa tgtatgaaag aaatgttaca ccacatatcg
     7501 tatttagcat tgaatccaaa ttccacgctt ggttaattaa agtacagaac aatggatacg
     7561 ccaataatac atagtataaa ctcatacttt ccctagattt aacccatatt gtcttatctg
     7621 agtagatgta agctgctatt ataacaacac aaatcagtaa agtgcagaaa tttaaatatc
     7681 tattttttct aaatgctggt aatgaggcta ttaacaaaca tactattgct agaatgactg
     7741 tcacaagtct aacgttcatc gtttctccca accagccgcc caaaggcttc aatatgcttg
     7801 atgagataat cattagaaag accattagaa actgtatgat tttccattta ctaatctgca
     7861 tgttaattat tattcctttc taattgtttt actactcatt caaaatttgt ttgtatgcct
     7921 cgtatgtgag ttttgcagat gccgcccagg tatacttgcg gtttacaagc tcatatatat
     7981 catcctctat tttgcttttc tcagctgggc taaggttcac atactttaca attgtgtctg
     8041 ctaggctctt agaattcaaa ttttcgaaaa gtaaccctat ctttccttcc tcggtttctt
     8101 ccataaaagc aggtacatta ctaacaatag ttggtaagca ataagtgtat gccataagaa
     8161 gcgctccact tccatagatt tctttatatg gaaatattgc ggcatctgca tttctgaaca
     8221 tactgtccat ttcttcatca gaaattctct ctggcttata tagaacacaa tctgaaacct
     8281 ttaaatcttc aatcattttt ttatagtcag tactatcctg tttaggatgt tgttgacctg
     8341 ctatggtaaa atgaactttc tttcttacat ccattggaag ttctgtgatt gcattaagca
     8401 gtacatcaat tcccttatat ggacgaattt gtccaaacaa gataaattca aacttatctt
     8461 tatcatacgc tctagatata tgtttaacat ctccatagat agcatgtgga caaacatatg
     8521 actttgcatt taattctggg aattcagcat ataaatgctg ttctgttact cgattgtgaa
     8581 caatgaaacc atcacaattt gaatatatat taaagtgctc ttggcgatcc ttttcacgaa
     8641 cctcatgtgg taacacatta tgaactgtca taattaattt tttatagtgt ctcttacata
     8701 aaacgtacaa tccgccttca tatttgagac ttttcatcaa ttgcaaatga acaatatcat
     8761 aatcaccaaa tcttatttcc ttccaaatta agaacaagct tttcagaaaa acaagtgccg
     8821 aaaacacttt atttgtactt ttatgataca aaatatgttt gctcttaatt ttaggatcta
     8881 aaggaggaac atcacgctta cacagcaatg ttaaatcaac atattgatta agttctttag
     8941 aaagatcaaa cgaataccaa ttctctggca tttggctttc acacatcaat accttcataa
     9001 tactcacctt ataaccaatc aacagaaaat tctgctttca gttttccttt aattttacgt
     9061 tttgctataa acatttccag tatctctcgc attttcgtct gatattttgt cgttttacca
     9121 taaagcgagt gattgtcccc tgttcctaat acatatactg ctgctttgaa tggaagaggt
     9181 tttaatggac gcccttgctc ttcacatgct ggaacaacgc ccccatgtcg ttttctaatg
     9241 atccatggat tatcatcaca atttgctgtc ataacttccg gatatgcctc aggcaggtct
     9301 tccactgcat attttactat acacgcactt cctgatgaga actttggtag aaccttcata
     9361 taatcgtctc caacaaagta cacgtatccc gttttgacat aaaatccatt ttcctgggga
     9421 tgggaattca catattccgc caaatgagaa cttacaagat catctgcgtc aaccatcata
     9481 gcgaaaccgc cgcccaactc tcgcaaacgc attgcgagag catgtgtttt ataaccctta
     9541 tcaatcattt tttcgtcttg atttgtggga ataggggcat ccttttcgct aacttgaata
     9601 aattcaactc tgtcatcata ttcatgcgtg agtttaggaa tttcatggca agcaacaatg
     9661 attttgaaat caggatcagt ctgattataa cacgaccata atgttcggtt aaataaggta
     9721 ctaaccatct cccaattgtt tgctgccttc gcactcatta aaggaataac aaaatacaac
     9781 atacaatctc tccttttagt aacgattcaa actacaataa tcatttacat caatatcttt
     9841 tttcaatact ctagccggaa cgcctcctac cgaacaatta ggttcaacat cattaatgac
     9901 cacagcatta gcccctacta ttacattgtc ccctattgtg atattaccta aaatttttgc
     9961 gccagtagcg atatatacat tgtttccaat tgtaggcata ccaggcttat tggatctacc
    10021 accagtcagt acattcgttc cgattataac gtgattacct atttttgtat cttttccaat
    10081 tacacaacct atgccaccgt ataagaactt acaattatct ccaatttctg tttgatatga
    10141 aattgagcta ttatgaagta cataaattag tgcctttaca atcttggcaa aaaactccat
    10201 tttgtgtaag taaaaccatc gttctacttt gtataattta tatgcattca tgttttatct
    10261 ccccatcttt ttagcaggta ccccccacac ttcgtagggt ggaatagacc tcaataccac
    10321 tgtatttgca gcaactacag caccttcccc aacgcataat tctgtgttag atttacaaag
    10381 aattttagca cccacacata aaacagcgtt cttttttact cgaatgatga tacgttcatt
    10441 actatctgct caaccacacg tcacattctg atagacttta acaccatctt caaggattgt
    10501 gtcagaatga atcacagtgc ctacagaatt atgaacaaat tcaacagagt tccctatctg
    10561 tgcaccttta ccaatacaac aaccaagaat tttggctatt tttctcgtgc aattatcaac
    10621 tatgtttaaa gcatatcgat tcctcaatgc cattaatttc gttacttcca tatcatttat
    10681 caccttccaa catgctaagc ataatttctc ccgactttga ccatctaaat tctgtagtta
    10741 cacgctgata tgctgcatct atgatatcgt tggtaaatcg tccgtctttt aagaatgtca
    10801 aaatttcatc gtcagattta gccaacattc catatctatt atccaaaata atattctgtg
    10861 ggccaggtgc attaacagca ataatcccga ggcgatagta cattgcctct agcaaaacca
    10921 taccccaaat ttcagtatag tttagattta caaatacatc cgataagcaa tataactccc
    10981 aaatatcttt gttaggaata cgctcaatta tttttactct attctgcagt ccaagtaaat
    11041 ttatctgctg cttcgtttgc tcgagtaaag caccagttcc taccatcagc aaataattat
    11101 gctcatcaag tttattaaat aaactaatca tatctaacgg tcttttatcc ttttcaaacc
    11161 gtcctacata taaaataaca cgaacattgc gaggaatatt ccatttttct ctgagagtag
    11221 taaaatctat tgcttcgtaa ttcttatgta actgttcaaa atccacacaa acaggagcaa
    11281 gtttcacttt atttactcct aattgtacta atttttcaga aactcgatca tttttggcaa
    11341 cacacccatt tttcttataa attttataat ttcgatgcgc caatatttca ataattgtct
    11401 ttatatattt attgccactt tgacttcgcg ttatcccatt ataaggtata tattttattc
    11461 cattttttac agtccatgag tatacatgag gtactgatag ttgaaggtcc gcaaattgta
    11521 ttagaacatc cgcattctta tcaaaattct ttgttgataa gaatccattt gatcctacat
    11581 aacgacactt gatatagtga atcatcgccg tattgcttaa ttgctctgtt ccaacttcct
    11641 catcactttt tacactttta tatacaataa cttcgtggcc taattcagct aaccctcgag
    11701 ctaatcctat ttcctgagaa ttataggacc ccttcttacc aaagttattc gtcattgtaa
    11761 ctactataag tattttcatc tatttttatt gcccccctac ttagattcaa tagtatagta
    11821 gatatacttt atacctccca agatataccg cctccatagt ctacgaggtt cctttaccag
    11881 tctaaataac cactctagac tagctttttc catccattca ggagcttttt ccaaagtgcc
    11941 cgcaaaaaag ttaaagccgg cgcctacacc gaacattact gtgttatgga tcttcttctg
    12001 gtgttcccac atccacattt cttgcttagg cgcaccaata ccaacccaca gaaaatctgg
    12061 atttaagttg ttgatttcct cgcaaatctt gctatcctct tcttcattta acggacgaaa
    12121 tggtggggaa tacattccaa caattttgat tcccgggtaa tctctttcta ggtttgcacg
    12181 aagcttattt aacacttcgt tatcttttcc accatagaaa taatgtgttt ttccctgtct
    12241 aacgccttct tcaaaaacca ttcccataat atctggtgca gcacatcgtt cacaggtaaa
    12301 tcctttgcgc cgtccaattt taacaatagg cataccatct attgcagcca ttgtagcctg
    12361 gctatacatt tcagcaattt tgggattttc ctttgccgaa ataatggccg gcactcccac
    12421 gagatcaaca gtcgcactta ccggagatgt tggcatggaa aaaacttccc ttacaaactc
    12481 gttctttgta gtagcacaaa cacctactat tccacacaca gtgaaatcac gttttctttt
    12541 atacatattc ttttccatct cagtacatcc ttactcatat gttcttcctc cgcatcctct
    12601 aattacacgg aggaagaaca gcttatatac ttattaaccc ttaatctgct tttcaatcca
    12661 attgtaggat tcacgaagac cctcttccaa agaaacagta ggctcccaat ccaaaatctc
    12721 tttcgcctta gaataatctg cgcaacgagc cttatcacct tcaggcttag aagtatcaaa
    12781 gaaaggcttg atgtctttgc cagaaatttc aatgatctta tatgcaattt ccttgatgga
    12841 agtacagtga gaagggccaa tctgaatatg accatgaccc caacccttat ccagggcgag
    12901 caccaatgca tttacgatat cattgacatg aatgaaagca cgtccctgct ctccgctacc
    12961 ccacacatta aaaggctctt caggattatt gattgcttta cgaatcagag caggaataac
    13021 ctgactacgt tcgccaaaat cagtaggtgt gccataaaca ttatggagca tcaggataca
    13081 gcacggaatt ccggtttctt tttccaaata ccccatctcc aactggccca tcagtttgct
    13141 ccagccataa gcagactcag gaagagcagg gaacaactcc tcttcacgga gaggctcagg
    13201 attcaaagta ttctgtctgg tcagagggaa ggagcatacg gtaccaacat agataacacc
    13261 cttgattttc tccttgccag ccttacggca acaatcaaaa acattagaat tgatcagatt
    13321 attgattcgg aacaattcgc cctggttctt aaacacatag tcaatgccag ccacaacatc
    13381 agccagatga actacatact cagtcgtacc aataaccttt ttagcctgct cataatcagt
    13441 cagatcagca ttgaagaaat gagtatccag gtcgatgaca ggatgcccat cttcgtcgtt
    13501 caggtactcc aactttcctc tccacagatt atcagctaca taaacatccc agcagccttc
    13561 cttcaccagt ctcttaacaa gattggagcc aatcataccg caaccaccgg taaccataat
    13621 tgtctttttc atttaacatt cctccaaatt ttattcgttt ccaaagaatt tatcttccag
    13681 catcaaacac caacaatgat atatcggcaa gtgatactct tgaatcatgt atgtttcaac
    13741 ttcaggagcc ttaacacaaa catctgctac cgtggcaagt tccccaccct ttgcgcccgt
    13801 caagccaata acttttatac cgagagccct agcaacaaca gttgcgctca taatattctt
    13861 gctatttccg gacgtcgaaa taccaaggaa tacgtcacca gatctaccaa agccaaacag
    13921 ctgctgtgcg aagacaccca aaccatcgac atcattaata taagcagtag acaatgcttc
    13981 gtgtgcgacc aaaggaattg ccgttaaacc acactcgaga tttttggcta attccggacc
    14041 tcttacaggg tcaattgcaa ctaacttatc ggcaaattcc ttacttacag gacgtggcgt
    14101 cttaaaccgc ttcatcaact cgccagcaat atgttccgag tcagcggctg atcctccgtt
    14161 tcctgccaca agaagtttac caccattcaa atagcattcc tccataatta ggtatccatc
    14221 gataatgtcc tgtttaatcg tctcaagtgt aggatatcgt ttaatcaata agtcaatatg
    14281 tttattaagt ttttccgcta acattgttac tccttatcca ttctgttttt caatataagt
    14341 ttccggcgtg tagtgaataa ctctagttcc accattttca aattcaaacg gaatatacat
    14401 caaatcacgc atagcccaac gaactgcatc ctgtttctca ggttgtacat agaaaaccaa
    14461 gaaacctcca ccacctgcac cgagcaactt tccgcctaaa gcacctgcag ccataccctt
    14521 ttcatatagt tcatcaatgc ttcctgtact aattgctcca ccagtacctt ttttcaatct
    14581 ccaagtaaca tcaagctgtc taccgaaatc atctaaattt ctgttcttat cagtaagaat
    14641 tgcctcagct tcatctacaa gctcatacat ctttttcagt cttactctct tatcctctgt
    14701 gccactgacg ttgttagcct tttgaacatc agaagaaaat ctagtgaatc cggtaaagaa
    14761 catcatcaga ttgttgttaa gctgcttttt acgttcaggc gaaataatga caggcagcac
    14821 ctcataacca tccacattga aattaattcg attgaaccca ccataagatg cggcaatctg
    14881 atcctgccat ccaccggctt cattacacag aactctttca agataaatgg cttcatcagc
    14941 aagtttcttc ttgtcagcat acttaccctc cagtgcataa aaagcattca acataccaac
    15001 tgcaaaagaa ctggatgtac ccagaccaga gcgagcaggt aaatcagctt cgtatgtaag
    15061 ccgaatttca tgcatatcga gcattttcat cgcattacga atcgcaggat gctcaatatc
    15121 ttcaatgcta gttactctct cagtacgaga ataagataac tcagcgctgt aatcaaaaaa
    15181 tctaggcaga tgtctcacat tcacataaca gtacttatca aaagtagtag aaagaactgc
    15241 accgccattc tctttgaaat aatcctccat atccgttcca ccgccaaaaa aagacatacg
    15301 aaacggtgtt tttgtaataa tcataaatac tctctcctaa tcttagtctc tctggaagat
    15361 atcacgggta tatacttttt ccttaatgtc atcaaggcaa gcatcgtagc gatttgctat
    15421 aacagaatca cacattgcct taaactgttc caaatcattc acaaccttac tgccaaagaa
    15481 agtctctcca tctttcaagg tcggctcata aatcacaaca gttgcacctt tagcttttac
    15541 acgcttcata acgccctgga tagaactctg gcggaagtta tcagaattgg atttcatggt
    15601 caaacggtaa acaccaatca caacaggctt ttctttgcca gcatcatact gatttgcttc
    15661 tgcgtaattg taataaccag ccatctgaag tacacgatcc gcaataaagt ccttacgagt
    15721 acggttagat tcaacgattg cctcgatcag attttcagga acatctgcgt agttggccag
    15781 aagctgcttg gtatccttcg gcaggcaata gccaccgtaa ccaaaggaag ggttgttgta
    15841 atgagtacca atacgaggat caaggcaaac tccattaatg atctgctgag tgttcaagcc
    15901 tttcatctct gcataagtgt ccagctcatt gaagtagctg acacgaagag ccagatatgt
    15961 attggcaaac agcttaacag cttctgcttc agtaaagccc atgaacaatg tgtccacatt
    16021 ctctttgatt gcgccttcct gtagcagtgt agcaaaagtg tgtgcatcct caaccaaacg
    16081 ctcatcatcc atatcggtac ccacaataat acgcgaagga tacagattgt catacagtgc
    16141 cttactttca cgcaggaact cagggctgaa aataatattc ttgctaccag tctttacacg
    16201 aacagaagcg gtgtatccaa caggaatggt ggacttaata accataatgg catcaggatt
    16261 tacttccatg accagcctaa taacagcctc aacagcggat gtgtcaaaat gttgagtgcg
    16321 gctattgtag ttagtaggag ccgcaataac aacaaaatcc gcagactggt atgctgcctt
    16381 tgcatccaat gttgcggtca gattcagttc tttctcagcc aagtactttt caatataatc
    16441 atcctggatt ggagatttac ggttattaat cagttcaacc ttctctggaa tgatatccac
    16501 cgctgtaacc ttatggtgct gtgccaacag agtggcaata ctcaaaccaa cataaccggt
    16561 gccggcgact gcaatgttat actgtttctt cataatattt cctctttcca ccactgctgt
    16621 ggtcactact ttcttaacaa aatgctgttt gtgaacagta acctgctatt tatataaaac
    16681 agcccgaaag ctgtgttcat tcatacttaa aacagccagc tcctacgccg gcaaattcaa
    16741 tttttcataa atcccctacc cgtttagggt tatattgcaa gtttgagtca tttttattga
    16801 cctcgtttgg ggttataata cattttcgca ttgaaatgta cctacatttt ccactcaacc
    16861 agtcaccaaa aagtttataa attcacttat taccctctat tcttactcca aaggtatgtt
    16921 taacatcgct ttattgacct attttcaccc caaatggaag tttgtttggt acattattgg
    16981 tctattccca ccccaaactg tctattttga gcgagttttt gttttttatc attgcacatt
    17041 ttcaaggctc aaattctaag cggatttact ggcagggaga cattggtaaa tacttgaaca
    17101 atttcacggc tttcatcatc acatcctacg ataataattc taattggata aagattatca
    17161 ttaataatct actttcacgt aaaaattctg aactaaataa aatctattga taatattttt
    17221 ttcttccact ttttgtatat cctacaggta tagtggattt aattatcccc aatgcctctg
    17281 aagtaacaga catcactaat tcaataacac attcactacc atagtttgta ggaacaaaaa
    17341 tgacaacaaa atctgcttcc ttatgtgatg tttttccatc tgtccaaatt ttattaccaa
    17401 tgaaatctag tttaaaatcc ctcttagaaa ctagttcact ctatattttt attcagtttc
    17461 attagttctg tttttttgtc ttcagtcacc atatacatat atatcttaat tatctatcca
    17521 tttcatccta cgcattcttg cgtaaataca attcactctg ttgagctgat ataaatcatt
    17581 ggtggtacaa agataaaatg ataactgttt ctatacctcc aaaaattcca gtaaatatat
    17641 ctatagatag tttcaaaaaa aattgtctta gtttcataat
//
LOCUS       DAPCMU010000049        17457 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPCMU010000049 DAPCMU010000000
VERSION     DAPCMU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.050"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            76..1017
                     /locus_tag="RD744_001346"
     CDS             76..1017
                     /locus_tag="RD744_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDU0557353.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(1167..2063)
                     /locus_tag="RD744_001347"
     CDS             complement(1167..2063)
                     /locus_tag="RD744_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDU0557354.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            2332..3672
                     /locus_tag="RD744_001348"
     CDS             2332..3672
                     /locus_tag="RD744_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0557355.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            3890..4333
                     /locus_tag="RD744_001349"
     CDS             3890..4333
                     /locus_tag="RD744_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDU0557356.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="RD744_001350"
     CDS             complement(4837..6255)
                     /gene="nhaC"
                     /locus_tag="RD744_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDU0557357.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(6658..7881)
                     /locus_tag="RD744_001351"
     CDS             complement(6658..7881)
                     /locus_tag="RD744_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDU0557358.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(7912..8751)
                     /locus_tag="RD744_001352"
     CDS             complement(7912..8751)
                     /locus_tag="RD744_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDU0557359.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(8768..9448)
                     /locus_tag="RD744_001353"
     CDS             complement(8768..9448)
                     /locus_tag="RD744_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557360.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(9450..10523)
                     /locus_tag="RD744_001354"
     CDS             complement(9450..10523)
                     /locus_tag="RD744_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0557361.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(10579..10817)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(10860..11093)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11170..11841)
                     /locus_tag="RD744_001355"
     CDS             complement(11170..11841)
                     /locus_tag="RD744_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0557362.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="RD744_001356"
     CDS             complement(11928..14606)
                     /gene="mgtA"
                     /locus_tag="RD744_001356"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDU0557363.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            15265..15702
                     /gene="ndk"
                     /locus_tag="RD744_001357"
     CDS             15265..15702
                     /gene="ndk"
                     /locus_tag="RD744_001357"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDU0557364.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(15724..16590)
                     /locus_tag="RD744_001358"
     CDS             complement(15724..16590)
                     /locus_tag="RD744_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0557365.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(16750..>17457)
                     /locus_tag="RD744_001359"
     CDS             complement(16750..>17457)
                     /locus_tag="RD744_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDU0557366.1"
                     /translation="YLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEF
                     DNAQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQ
                     HDQVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLS
                     DKLNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTT
                     NLYPVTRTDVEQQKNNQ"
BASE COUNT         5077 a         3577 c         3090 g         5713 t
ORIGIN      
        1 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
       61 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
      121 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
      181 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
      241 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
      301 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
      361 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
      421 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
      481 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
      541 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
      601 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
      661 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
      721 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
      781 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
      841 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
      901 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
      961 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
     1021 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
     1081 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
     1141 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
     1201 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
     1261 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
     1321 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
     1381 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
     1441 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
     1501 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
     1561 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
     1621 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
     1681 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
     1741 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
     1801 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
     1861 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
     1921 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
     1981 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
     2041 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
     2101 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
     2161 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
     2221 ttctgaatag ttgtaattgt ctgaaaattt gttatcatta gtcattgtta aatattgcta
     2281 tttattctta atataaattt tgattgtgaa tttaaaagaa agaggtcatt tatggaagat
     2341 aaaaaattga aaaaacaact tagctccaga catatcacca tgttagcttt aggtggagca
     2401 atcggtgcag gtctatttaa aggaagtggc gaagcgattg ggatcgccgg cccttctgta
     2461 ttgattgctt ttttgattgg tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa
     2521 atcgttttaa gcggtggcga cacgcatcac ggattatctg gtttgatacg cccttatcta
     2581 ggtgctcatt ctgctgattt tactgattgg gtctactggt ctatgtggat tatcaatatc
     2641 atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt ggttcccgca cattccagcc
     2701 tgggtatttg tttttatgtt ggctgtttta actacgatca tcaatcttta ttcggtacgg
     2761 ttattcgcag aaacagaata ttggctggct tttgctaaaa tcagtgtcat tatcttactg
     2821 atcatttttg ctacttatct tgtcggtcag caaatgttgg gcacaggcgt tttccctacc
     2881 cttcagcaat tgactgacca tggtgggttt actcctcatg gtatgaaggg aatcatcagt
     2941 tctttgcttg tagttatcta ttcttacggc ggttctgaac tgattgctat cacggtaagc
     3001 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggtgtcat gggaagaatc
     3061 atttcctttt acatcattcc tttgtttttg cttttgattc tttttccttg gaatacttta
     3121 gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa aaatgaatat cccatttgca
     3181 gcggatatcg tcaattttgt gattgtcctt gctttgttct catctatcaa ttcgggtgtg
     3241 tatgcatctt caagaatttt gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa
     3301 ctagctgttt taaacaagca tcaagtaccg caacgtgctg ttttattttg taccagtgtt
     3361 ctttatcttg gtgtagctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
     3421 ggttcccttt cgtatacagt attattgatc tggatcatta tcagtgcggc atcctttgta
     3481 ctggcactaa gaaaaggaac attatgggat aaaggaatca gcttttttgc attagctgtc
     3541 ttaggattga ttttccttgg tatcctgttc acgaactcca ttggggtaac tgtattgact
     3601 ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg
     3661 acgaatgaat gaaatcgata gaaaatttta ttttatttat gtggaataga tggagctatt
     3721 gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt tttccgaata aacagtctca
     3781 tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa aataattgat tgttctccat
     3841 gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta
     3901 ttggaagttt ttgttgatga tcggtgtttc aacggtagtt atgtttggta tgatgtactt
     3961 gaatgtctat gcgctcgatc atatcttctt cagtcgtaca cgagtattca tggcgttgat
     4021 gatgggtgca gtcatggcag tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa
     4081 aaagataaat gttatgatct tagccgctag tcttcttgtt tttgccggtt ccttgtttat
     4141 ggtacgaagc caaactactg tcaacgacac agcttggatg aaggccatga ttcctcatca
     4201 ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt
     4261 agcggaagaa atcattcagg cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa
     4321 agaaatggaa tgaccattat ccgtccaaat aaaaaaagag gatgttccag aagttatcag
     4381 cttcaagaaa taagaagaag ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc
     4441 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcctat ttttcgtttg
     4501 gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat
     4561 cagcgttctg acctgagtat tagagagaca aatggaaaaa tagttcctca tattttgcca
     4621 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa
     4681 aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa tggctttcca catttttgta
     4741 taaatttggc ttaataccga gaaactggct tttgaatacc gtttattcgt aaaagggatt
     4801 gtgacaaaag tcttgtcaca atcccttttt atgtttttat tgttctaatt gcgctgtctt
     4861 gcttactgct tcttgcggtt tcacaaattt ttttgagatt ccccaacctg tgaaggcact
     4921 gaccatcgaa aacacaggag aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt
     4981 aggaatacca aatgtacttg ctacgaaact tccggcaatt ccccaaggca ctaagtagtt
     5041 gattactgtt ccaccatctt ctagcgttct acctaataca gttggatcaa atcctgcttt
     5101 ttcataagtt tctttaaatg cattaccagg cagaataaca gataggaatt gttcaccaat
     5161 aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt
     5221 catctttctt gatactcgtt ccatcacggt tgagatcaag ccaaattcga tcaacaatcc
     5281 acctaatgac aaagttagaa taatcaatga cacagtaggc atcatacttt cgattcctcc
     5341 gcggctaagc agtgaatcga tttgttggtt tccagtattt gaaacaaacc ctgattcgat
     5401 gaccccagcc aacttcgtta aggaagtttg tgggttttga ataaaaatca ttcctactgc
     5461 tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc tttttccatg cacacagtaa
     5521 cattaaaaat aaaggaatca gtgaccagat tgaaatagta aagtttgctt ctagaacatc
     5581 agtcacttca cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa
     5641 taaaaagaaa gaaacaacaa atgccgggac agttgaccac atcatatgct tgatgtgttt
     5701 gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt gattcggata atggagacat
     5761 cttatctcca aaaacggcac ctgaaacaat agctccaaca accagcgctg gatttaagcc
     5821 tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact
     5881 ccctacagcg ctacctacga ttgcacacac aacaaataca gaagggacaa accactggac
     5941 actgatcaat ttgaacccaa tgaccattag agttggaatg atccctgctt gtatccatac
     6001 agcaatcaat gaaccgacta aaataaaaat aaagattggg atgattcctg gtttgatgcc
     6061 attgatgatt ccttcgtgca gccaatctgt cgggtatctt tttatcactg catacaacat
     6121 gaccattccg atcgctgcta agatcgtgat attaggtgag acctttaatc cgacaacgcc
     6181 aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt gcttctaaaa aacttggtgt
     6241 tccttttttt cccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta
     6301 agatatcgga ttactccgcc gcaattcatc cgcgattcct cctttataaa aataaaaaga
     6361 cctgttttaa actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt
     6421 ttttcacttt caaactttta ttcaaatgta tggactttcc atgtaattcg tcaattaggc
     6481 ttgatcggtt cacagcgacc accgactttc tgaacaaaac acctaaccgc tactgcgtga
     6541 aaagttgact tgtacgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata
     6601 ttttttcatt tttctgaaaa ttatttttat aactttcaca cacaagttta ttttttacta
     6661 gaaagtagcg ccctcttgtt tttttcttgc tactatttct tgatgttctt gcttgattct
     6721 ttctaatagc tttggctctt tcaacagatc aagagctgta ttggcgagta gttctgctgc
     6781 aatcgcaata gaagcaaggc ctttgtcact tttagcagca gctttgaatt cttctgtgtg
     6841 tccggcaata tatgtctcag agattgctac cgtaggctgg attgttggta tgacttggct
     6901 gacatttcct acatcagaag aaccgagact tgttctttgt tccgtttcta tttcttcatc
     6961 tggaacacct gcttcttttg catgctggaa gaataagtca tcaaaagaag gagtaacgat
     7021 gacatcatca actgcatttt ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc
     7081 agcacctcgt acgatatttt ccactttttc atatacttta tctaatgttt gccgatttcc
     7141 tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg acgttggcag ctactcctcc
     7201 gtctgtaatg atcccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat
     7261 tccattaaac acttggatca atgcttccaa agcatttact cctttttcag gtgctgctgc
     7321 tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga tcattcgcta aagattctgt
     7381 cgttttccca taacgaaaag ctggatggac gcataacgct acatcgacat cctcaaaaaa
     7441 accttctcga acaaagctgc ctttagctga tccgttctcg cccccttctt cccctggggt
     7501 gccatataca cggatttctc caccagtttc atcaatgaat tgcttcacta tgctggcggc
     7561 taatacggag taagtaccaa acaaattatg cccgcatgca tgaccaatac ctggtaaagc
     7621 atcgaattct gccaaaaagg caattgtggg accgggttta ttggaaatat aacgtgcatc
     7681 aaaggctgtt cggtgtccag ctacttcttt ttctacccaa aacctttctt tttctaattg
     7741 tttaatcaac gctgctgagg aataaaactc atagttactt acttcaggat gttcgtggat
     7801 atctagagca agtgcttgat attctggtaa acgatattgg attagatcct tcaattggtt
     7861 tgttgagatt gttgttgtca taaaaaattc ctcctatgct agtcattttt cttattttct
     7921 cccgaatgtt tcccatgcag gcacgcttga tccttttgat gttttctcga ttactttgac
     7981 tgtgtcttct gtttgataag cgtctactat tttttgatac gttttattgt tttcatcttc
     8041 ttttcttgca acgatgatat tcacataagg acgtgcatgg tcatccactg gttctaagaa
     8101 gatggcatca gaagcaggat taaatccagc atctacagct acaccgctat tgatcactga
     8161 aatgtctaca tctgacaaag cacgtgctgt ttgggaagca tctaattcag aaatgtctag
     8221 attttttcta ttctcggtaa tatcagaaac agtgggcgct tgtccttttg ccttatccac
     8281 tttgatcaat ccagcagttt gcaataacag aagagctctg ccgccatttg taacatcatt
     8341 agggattgct acttttgctc cgtctttgat atcagctaca tccttcaaag tgttagaata
     8401 aattcccagc ggtgcattga cagtatttcc gatcgatact aaatcagttc cgtgttcttt
     8461 attgtaattg tctaaaaaga attgatgttg gaaagaattc aaatcaatcg atccatctgc
     8521 taaagccaca tttggttgat tgtaatctgt aaatttcacg tattccaaat taatgttctc
     8581 ctttttcaaa cggtctttca cgttgtccca tacatctgta tcttctccaa taatccctag
     8641 ttttacgata gtttcttttt tgtcagttgt atctgctttt ccttggctac accctgccaa
     8701 ccaaataatt gctacaaatg taatgactac aaaacgaata atttttttca ttttttctcc
     8761 tccactttca atgtgtaatt tttttaatca gttgattact gataaattga cttaaaaaaa
     8821 ctaagattag aatcaataat gtagccacaa tcgttacatc tgtttggaat cgattatacc
     8881 ctctcgatat agcaagattc cctaatccgc ctgcacctac tgctccagcc atggtagtca
     8941 gtccgatcac gttgatgata gtcaacgccg agacccgaat gataccaggc aaaccttcta
     9001 acaaatacac acgtaaaatg ataccgattg gactggttcc catcgcttct gccgcttcga
     9061 tcaccccagg atcaacttct agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa
     9121 ctccaaaaat caatggcact aaagctgctt tcgcaccaat cgtcgttcca actaagaaac
     9181 gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg gatggaacga atgatattga
     9241 tcagcttatc aagtatttca aatagcagac ggttagctag taatccatta gggcgtgtca
     9301 ctactaacgt cacacctaag atgattccaa tcatcccgcc aatgaatgct gtccaaaaag
     9361 acatatatag tgtttgcttc gtcgcttcga caaattcatc cggaatcaaa taaacattgg
     9421 gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc cttcgatcac tttgatacga
     9481 ctatctaaat cgtttttttg taaaatattt atttttacac cagatttgat caaataatcc
     9541 aatgcttttt tccgctgttt ggtttctcca gctaaagtaa cgatcaaact ccctaacggc
     9601 gtttcttgta ataactctac attgccatat aaaatattcg ctgttacttg atatttgctg
     9661 tacagatgag cgattagagg ttcgttcgtc tgatttccaa tataacttaa ttccaccagc
     9721 catttattgg cgatctgatc ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg
     9781 tcaagatgag tagctgttcg aataaaatct ttggtcaatt cttcttcagg attgctgaag
     9841 atctggatgc tgctaccttt ttcaacgatt cttccatctt ccataaccgc taccttgttg
     9901 cagatttctt tgacaacttg catctcatga gtgatcagga cgattgttaa acctaactgg
     9961 cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg
    10021 gttgcttcat cacataataa taccttaggt tcattcgcca aagctcgtgc gattgctact
    10081 cgttgttttt gtccccccga cagttgttta ggataagcag atgctttttc ttccaaaccg
    10141 acaagttcca gtagttcatt cactttttga cgacgttcct gtttcgattt tccagaatac
    10201 tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt ccattagatt gaaatgctga
    10261 aagatcatac cgatggcctt tctttctttt cgcaattctt tgggagagag ctgactcaga
    10321 ttcgtctgat tgacaatgac ttctcctgct gttggtttct gcagcaaatt catcacacga
    10381 actaacgtac ttttccctgc acctgagtaa ccgacgattc cataaacatc gcctttttca
    10441 atcgttaaat ctacagaatc tactgcctgt atctcttttt ctttttgttt gaaacataca
    10501 gaaacatctc gcaattcaat cattaaaatt cctccttatc taaaaaaagc gtcctcaaga
    10561 aaatgaaaat gtcattttct tgaggacgct tttttgcgtg ttaccacctc atgttcaagc
    10621 tgtgttcaca cacaactcct cttcaagtac gatcatactc tagcaagata tcggtcgcag
    10681 ccgtcatcgc cttacatcta tgctcgggat gaattgctcg atggccattt tccaatagtc
    10741 gttccttgct tcttctcagc taccgaagct ctctgtcaaa ggttcttcta tcgtacttac
    10801 catttcaatg caaataatat tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt
    10861 aaaaggacga cgtatcgcgg taccaccttt tttcggaagt ttccttccct caagcccaac
    10921 atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa cagttccgaa tttgcctact
    10981 tatcgacaaa ttattctcca aggccatttt cagcttttct tacttgactc ttctcaccag
    11041 ccagagcctc tctgcaaagt ttccaaactt actttccttt tcaacgaaat cgatattttg
    11101 ttgttgggat taatctacat gaggaaaact gaaaagtcaa gtacgaaatt caaattattt
    11161 tttctatatt tatttttctg tttttttatt taacatttca ataacagttc gaagatcttt
    11221 tccttccgca agaagtttca atttttcctt ttcttcgacg gtataatttc ggacaaaact
    11281 ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa
    11341 agaatcttcc actgcaaagg ctgaactagc ttctaatcct gttcgttcca aagctgtcag
    11401 ataaggaatc ggtgaaggct tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa
    11461 gtaatcagct aatttaaagt gatccaagat atctgttgct cttttcgtaa cagtggtcgt
    11521 agcaatgcct agtttcatat gacgttcttt taagtctgcc aatatttctt ttgcatatgg
    11581 ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg
    11641 gacttcctgt actttttcag ggctacctgc tgctttcact aaaatatcaa aactttgttt
    11701 ccagtttttt ccgccccaag cgtcaacgat ttcttttggg atatggatat caaattgctg
    11761 aaatccccaa agccagccat cacgatacgt tttttctgtg tcaactaaca aaccatctaa
    11821 atcaaatatt gcacctttca tctatacatt aatcctttcc aaaacaatac acatagttta
    11881 gcacgactgg tccaaaaaga acagaagcaa agaaccagcc ttctcgttta taagagttct
    11941 tggaatcgac ggatatagat tttcttcaca gctgtcacaa gcgccagata agcgatgact
    12001 gtagcaaata accataacca gtaattgcca ggaagtggca tcaagcctaa ttcagcacca
    12061 aaacttgtaa aaggaaggac agaaccgaca ataattccta atgtagtgat aacaaacatg
    12121 gcaaatgttg cgttgctttg gataaatggt actttcggtg tacgcagtgc atggagcact
    12181 aaggtttgtg tccatagtga ttcgacaaac catcctgcat ggaataatgc gatgaaaatc
    12241 actcgctgtg aactatccaa tgtatggaaa cttccgccca ctacagcagg acaaataaca
    12301 aaatacatca acaagtaagt agtaatatca aatatcgaac ttgttggacc aaagtaaacc
    12361 atgaattccc caatactaga tgcattccat ttttttggct cgtccaaata ttctttatcc
    12421 atccgatccc atggcaagga aatgcaggaa acatcataga tcagattcaa aaacaaaagt
    12481 tgtaaaggca gcattggtaa aaatggcagg aacgtacttg caatcagcac ggaaaacata
    12541 ttgccaaaat tagaacttgc tgtagccttg atatatttca tgatattccc aaaagtttct
    12601 cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct
    12661 gcagattctt tcgcaatatc cacagctgta tctacagaaa taccgatatc agccactttc
    12721 attgcaggag cgtcattgat accatctcct aaaaatccga cagtatgacc atttttacgt
    12781 aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt ttacgaatac ttcatggtct
    12841 tcagctactt gtgctaattc atgatcactc atttccgaga ttttctcacc ggtaaccaat
    12901 tcattgatct ctagacctac ttgacggcac actgaacgtg taactaattc gttgtcccca
    12961 gtcagaactt ttacagctac accatgttct ttcagagctt tcaacgcttg tttcgttgtt
    13021 tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa ccatgtctga ctcatctttg
    13081 actgaaaatt ctccaacgac gcttggattg gtcttttgtg cgacaccaat cactcgtaag
    13141 ccatcttcat taagtgactg tacttttgca agtacggctt cttttcgttc ttttgttaat
    13201 ggtgtaacag taccgccaag atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg
    13261 ccttttgtga tcatttgtgt tttaccagct ggatcagcaa ccacaacact catacgacga
    13321 cgttcaaaat caaaaggaat ttcatccact ttttgataat tgatcttatc tgtatctaaa
    13381 gtttctgttg cttcatcaat gatagccaca tctaacaaat ttttcagccc tgtttgatag
    13441 tagctattta gatacgcatg gcgcaaaaca cgatcatctt catttccttc acaatccata
    13501 tgatattcaa gaatgatttt atcttgtgtc aatgtccccg ttttatccgt acataacaca
    13561 tcgattgcac caaagttctg gatggcattc aaatttttga tcactgttcc ttttttggcc
    13621 atcacacttg ctcctttgac caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt
    13681 agaccaactg ctacagatag tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc
    13741 ccattgatga cgataactgc tggagccatc aatgacataa atttgatcag tagcatggat
    13801 gttttatgga tgcctaattc aaagcttgat tgaattggtt tttcagatag cgtttctgct
    13861 acatgaccaa atagtgtttc atttccaaca gaaacaatga ttccttccgc acttccactg
    13921 atgacattac ttcccataaa gacaagattt tcgtaacttg tttcgctttg ctgatcgtct
    13981 tcacatactt tcgcatgctt ttctactgga tagctttccc ctgtcaatgc tgcttgggaa
    14041 atgaataaat cctttgcgga caataaacgc atatccgctg gtatcatatc ccctgctgaa
    14101 agccgaacaa gatctccaca gacgatttct tccataggaa tctctgtgta ctttccatta
    14161 cgttttacag ctgccgttac tttcacaagt gatttcagtt tttcagccgc ttgattggaa
    14221 cgtacggact gaatcaacgt cattgtcccg ctgacgataa ccatagtgaa gataatggct
    14281 acaccgaaaa gatctttatc tccaggagct gctaatacat attccgtgat gaatgaaata
    14341 attcctaatg cgatcaacac taatgtaaat ggcgtgatgt atgctttcaa tatttctaca
    14401 atgaatggtg tttttttgcc atatgaaatc gcattttcac cgtaattttc tcttgaacgt
    14461 tcgatttgtt cttctgtcaa tccatttgct gatgcatgga atagttcaaa taattcttgt
    14521 ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat ctttcttttt ttccatctca
    14581 atctctcctt tgtgtccaaa agacagctgt tctcgctgct tttggtcttt agctattcat
    14641 cggatcgata gagaaaaaac tgaaaggggc tgagaacaga tagataatag ccctggcctt
    14701 tcctctagat ccttgttttt cgaatgactg tcatccattt gttctcatct cctttactaa
    14761 gtaattttaa ttcatgactt ccaatcattt ttcgaatagt tatttttcca attagaccac
    14821 ctccttttta tttttcaccg atggggattt gcggcaacaa aaaaacctct cgccaaatac
    14881 tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct cagatctcat cgttgagttt
    14941 tgacactgta caacgtaaaa agcagatacc tgcttttagc taccttatga aaagccttat
    15001 ctcgacaatt cctgttctac ccattggcgt ctctcgacgt ttttgggcag tagcctatgt
    15061 ttgtatagga gcctcaccta acaaatctta ttcggttttc cttcgttaat ataacaacta
    15121 aaaaagagga tgtcaaatca attttccatg tttttcatac tttttttaaa atacacacgt
    15181 tatattcctg tctcacgcgg ttttatttgt ctcattttcc acctcccgct ataatagaaa
    15241 tatagtagga aacgaggtgc tgttatggaa agaacacttg ttattatcaa gccagacggc
    15301 gtgagacgcc atttagtcgg aagcatcatc caacgttttg aagcaaaggg actggccatt
    15361 gctgaaatga agtttgaaac tatgactcct gaattagcaa aagaacatta tcagcacttg
    15421 actgagcgca gtttttttga tgaactgatc gattacatga cttccggtcc tgtcgtttat
    15481 cttgtgctgg tcggcgagga agtgatcgat atcgtccgta agatggttgg agcaacaaaa
    15541 gctgccgatg ctgttccagg tacgatccga ggagactacg cgctgccggg gactgaaaac
    15601 atcattcacg cttctgattc ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg
    15661 ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct
    15721 ggtctagttg ctgggttcag caatggccag cagttttttc tctaaggacc tccgttgatt
    15781 ctttcctaac atatattcga gcactgtgaa gaattcttgg tagtctttcg tcttttcttc
    15841 ccctaaagca gcagaaatca atctttgata ataatctgcc atcaatgttt catagatcac
    15901 tgtacttctt tctttatgtg ctaagctccg tttcatatct tcattcacac tacgtgcctt
    15961 tttcaaccga ccattttcta tataaaacga agtcaggttc attaaaaaag taaagagata
    16021 ctgctcatga ttgttcaact gtgctaatcg tcgcattctt tttttgattt tcaaataatt
    16081 gttctcaatg aaccgatcac tgaatatcca aggaaaatca gctagaatag atagttcaaa
    16141 ttgaccatag tctggtatct gcatcaagta ttcttcaatg attcggcatt cataacgtaa
    16201 aaattcaaat tctgtctcag atacttctgt tttttcgacc atgtgaagat tcaaaaccaa
    16261 ataatacaga tcttctgttt tcttaaaatg cctacggtta ttgctgatct ctttataagg
    16321 atcaaagctg tcttcttgta cccgatctag cattcgtctt tttttcttta actttattgg
    16381 ttcatccagc tggtttcgca tccattcata ttctgcaagt gtcatgttca ttcgctctaa
    16441 aaatgatctc aatgttacaa atgacatttt tttcactgaa tttgtctcaa tcccgactag
    16501 agaagtctta aaacagattc cttccgctaa ttgggactgt gtcaacttct tgttgactcg
    16561 aagtttcttg atcaattcac catcatacat aatagttctc ctttttaatt taaatttttc
    16621 cacattcaat aaatagatac gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt
    16681 tttttcataa aaaataccaa ccttcaagca ttagaatgtt gtctaactac tgaaggttgg
    16741 tttgattgtt tattgattat ttttttgctg ttcaacgtct gttcttgtta ctggatataa
    16801 gttggttgtt gttttattac cattttttga aaagacactt gtcgaatgtt cgcctttttg
    16861 cttttcgatt gcttcattac gttccgcttc atttttatag tcatattttt tcggatcaat
    16921 cgtctcaaat ccgtttggca cataaaaccg caagagattt tcctgattca gcttatctga
    16981 aagacttaac gcagtcttga ctttttcctg atctttggag atttcctcat cgattcctgc
    17041 tgctttcgta tcgatgattt ctcctgtctg attattgtaa gcttttcctc ctaggacggt
    17101 gtatttgggt gtcacaaagt tcccatttcg aaaagcaact acttgatcat gctgagaaga
    17161 taataagtct gtaccaaaat gaagataatc tttatcatcg attcccaata gatgtagtaa
    17221 tgtcggcaaa acatcgattt ctccaccgta ttcatgattg actgtccctt tagtataacc
    17281 aggcatatgg atcatcaatg gcactcgttg catctgtgcg ttgtcaaatt catcccatgt
    17341 atctggatct ttaccaagag cgcttgctaa atctttatta tctgtattcg agatcccaaa
    17401 atgatccccg taaatcacaa agattgaatt ttggtaaata ccagattttt tcaaata
//
LOCUS       DAPCMU010000050        17430 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPCMU010000050 DAPCMU010000000
VERSION     DAPCMU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.051"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            163..393
                     /locus_tag="RD744_001360"
     CDS             163..393
                     /locus_tag="RD744_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDU0557367.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            490..1791
                     /gene="murA"
                     /locus_tag="RD744_001361"
     CDS             490..1791
                     /gene="murA"
                     /locus_tag="RD744_001361"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDU0557368.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1804..1980
                     /locus_tag="RD744_001362"
     CDS             1804..1980
                     /locus_tag="RD744_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDU0557369.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2115..2411
                     /gene="yidD"
                     /locus_tag="RD744_001363"
     CDS             2115..2411
                     /gene="yidD"
                     /locus_tag="RD744_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDU0557370.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2475..2942
                     /locus_tag="RD744_001364"
     CDS             2475..2942
                     /locus_tag="RD744_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557371.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3027..3911
                     /locus_tag="RD744_001365"
     CDS             3027..3911
                     /locus_tag="RD744_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0557372.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4025..4261
                     /locus_tag="RD744_001366"
     CDS             4025..4261
                     /locus_tag="RD744_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDU0557373.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4349..4429
                     /locus_tag="RD744_001367"
     tRNA            4349..4429
                     /locus_tag="RD744_001367"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4383..4385,aa:Tyr,seq:gta)
     gene            4436..4507
                     /locus_tag="RD744_001368"
     tRNA            4436..4507
                     /locus_tag="RD744_001368"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4468..4470,aa:Gln,seq:ttg)
     gene            4632..4802
                     /locus_tag="RD744_001369"
     CDS             4632..4802
                     /locus_tag="RD744_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557374.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5022..5729
                     /locus_tag="RD744_001370"
     CDS             5022..5729
                     /locus_tag="RD744_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0557375.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5789..6433)
                     /locus_tag="RD744_001371"
     CDS             complement(5789..6433)
                     /locus_tag="RD744_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDU0557376.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6560..6847
                     /locus_tag="RD744_001372"
     CDS             6560..6847
                     /locus_tag="RD744_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557377.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6955..7275
                     /locus_tag="RD744_001373"
     CDS             6955..7275
                     /locus_tag="RD744_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0557378.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7356..8669
                     /locus_tag="RD744_001374"
     CDS             7356..8669
                     /locus_tag="RD744_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDU0557379.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8666..9385
                     /locus_tag="RD744_001375"
     CDS             8666..9385
                     /locus_tag="RD744_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDU0557380.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9513..10064
                     /gene="raiA"
                     /locus_tag="RD744_001376"
     CDS             9513..10064
                     /gene="raiA"
                     /locus_tag="RD744_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDU0557381.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10286..12820
                     /gene="secA"
                     /locus_tag="RD744_001377"
     CDS             10286..12820
                     /gene="secA"
                     /locus_tag="RD744_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDU0557382.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12946..14047
                     /gene="prfB"
                     /locus_tag="RD744_001378"
     CDS             join(12946..13017,13019..14047)
                     /gene="prfB"
                     /locus_tag="RD744_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDU0557383.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14152..14838
                     /gene="ftsE"
                     /locus_tag="RD744_001379"
     CDS             14152..14838
                     /gene="ftsE"
                     /locus_tag="RD744_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDU0557384.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14822..15715
                     /locus_tag="RD744_001380"
     CDS             14822..15715
                     /locus_tag="RD744_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557385.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15867..17315
                     /gene="cls"
                     /locus_tag="RD744_001381"
     CDS             15867..17315
                     /gene="cls"
                     /locus_tag="RD744_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDU0557386.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5824 a         2953 c         3782 g         4871 t
ORIGIN      
        1 cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa aaatgtaagg
       61 ggattcctat atttttaagt atacttgttt ttccttttat acattttatg gtattatcat
      121 tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt atacggaatt
      181 gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt ctggtcttta
      241 cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat tagaatggca
      301 atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt agaagttatc
      361 gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac tattttaaaa
      421 gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga attttttgga
      481 gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg aacagtaaga
      541 attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt agcagaagaa
      601 ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat gaatcaagtg
      661 attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt aacaatcgat
      721 gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca
      781 atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc tatgcctggt
      841 ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca agctttagga
      901 gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt gatcggtaac
      961 acgatttatt tagatttccc tagtgtaggt gctactcaaa atatcatgat ggctgccgta
     1021 aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat cgttgattta
     1081 gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga aacaatgcgt
     1141 attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa
     1201 gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat tgcagatgct
     1261 atctctgaac ataaccgccc attgatttct aaattgatcg aaatgggtgc agaaatcatt
     1321 gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac agatgtaaaa
     1381 acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc tatccaatta
     1441 gttgcagaag gtacaagtgt agtgactgaa acagtgtttg aaaatcgttt ccaacattta
     1501 gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat
     1561 ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc agcagcagca
     1621 ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt gaattatcta
     1681 gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt
     1741 gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta aggaagtggc
     1801 tgaatgagta accaacgata tatcttatta actttgataa agattttagt ggtgatctta
     1861 ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat cggtggcggc
     1921 aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt ttttcattga
     1981 taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca gaacatgaca
     2041 aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt aaaagaaaag
     2101 aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat cagcgcttta
     2161 tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat tatatgataa
     2221 aagcaattca aacccatggt gcactaaaag ggacggtaat ggggactgca cggatcttac
     2281 gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc caattaacgc
     2341 gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat acgagtagca
     2401 ctgagcgata aagaaaaaga ggaaaattat tgtcaaagta acaactccgc aactacttat
     2461 atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg tccttgttat
     2521 tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg agtggctgga
     2581 tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg gaaaaagaat
     2641 acgaaatcgt caatgtagca gtagccccaa cgtatcaagg aaaggggatt ggcggaaaat
     2701 tacttgagac agcttttcag aagctttcac aactcgcttc cagtcaaact cagatcatca
     2761 tacgaaccgg cagtactagc agtgcggcgt tacatctcta tcaaaaaatg ggctatgtgg
     2821 agattggtcg agtcaaagac tattttatcc atcattactc tgagcccatt tttgaaaatg
     2881 gagatcaatt aagggatcaa gtaattttag caattcactt acctaaaaat agctttaact
     2941 aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt acaatagctt
     3001 tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt ggtggatcgg
     3061 ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa tttgcaacgc
     3121 ctgaatcttg ggaagaaatg aaagcgcaat tatttcaagt ttataaaaaa atactcgttt
     3181 cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat gagaaacagg
     3241 tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc cgagatttgg
     3301 aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc ggaatggctg
     3361 aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg gcaggaactg
     3421 ggattggcgg ggcgttgttc acgaaaggac aaatcaataa aggggcacat ctttatggtg
     3481 gagaatttgg gttgatgttc ttaaatggcg ataagacatt cagtaaactt ggtacagcgg
     3541 tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaattct tataccggaa
     3601 aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa gttgagagtt
     3661 tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat ccagaagtga
     3721 tcgtattagg cggcggtgtt tctgcaaaag aagggctcat tgatgagatc aaaagccgaa
     3781 tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt ttattatgtg
     3841 aatacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct gtacgtcaag
     3901 gggagaatta agagctttag agaaggtgca gcttgcataa aagaagtgaa tgaaatgaat
     3961 aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata tctgagaggt
     4021 gaaaatgatg tcaaaaaaaa ataaagatgt tgaagtgcgt atagaagaag tgaaaaaaga
     4081 tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa tgattggtga
     4141 gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag attttggaaa
     4201 agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc tgcacgaata
     4261 aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat taggtattat
     4321 aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa acgcgacgga
     4381 ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat ttcattgggg
     4441 tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc gaatccagct
     4501 accccagtat accacaatcg taagctctcg ttagttcgag agctcttttt tttgctttgc
     4561 taccttgttc cttgaagatt ctgctgattt aagatagagt taagttaact acaagaattg
     4621 gagggatcca catgaaatgg aaagaaaaag tagacgaagc agctgaaaag ttatatgatt
     4681 tggtcaaatc agaaaaatac aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg
     4741 tccaggtaaa atcaaaacgc ccaacgaatc atacaaaaaa atggatggct aaaaaccgct
     4801 aataataaca aaagatccat aagtcggcat tttcatcgat tttagggtct tttgttattt
     4861 tctaggttga acatatctaa aaaattgttt ctgacaatag gagtaggaag aacgatgtgt
     4921 gtctaaatcc tctgttgatg gatagaaacc tttatctttt tcagttgtga atcaggtata
     4981 taccagttat aatagaagaa acgaaaggac gggtgtacgt tatgtcgaat caattacctg
     5041 tatatataca gattcatgac caattaaaaa atgaaatcga acaaggtata tggaaagtcg
     5101 gagaccgtct tccttcagaa agagaattag cagtgaaatt caatgtcagc cgtatgacgt
     5161 tgagacaagc aattcaaaac ttggcagatg aaggaatctt agaaagaaag atcggttcgg
     5221 gtacatatgt tgcgcgagaa aaagtacaag aaaaaatgtc tggtgcaaca agttttacag
     5281 aaattatgga atcgcaaaat cgagtaccgt ctagtcggac aatctcttat ttcttgactt
     5341 cgccaagctc aagtgaaatg gaaaaactaa acttgagtaa agatgacacg atcttgcgaa
     5401 tggaacgtat tcgtttcgca gatgatgtcc ctatttgttt tgaagttgcc agtgtgccac
     5461 aaaagctgat cgatggctac agcaaagcag agattactga atcgttgtat cgcacgctag
     5521 aagaaaaagg cgggaaaaag atcggtccag cgaatcaaac gattactgct gtgttggctt
     5581 ctgagaaaat tgctgactat ttagaggtaa aacgaggaga tgcagttctt cgtttgcgcc
     5641 agatttctta ttttgaagac ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa
     5701 atcgttttga gttttatttg gaaaaataaa aaacaggtga tccctgattc cttacttaga
     5761 aataaaagag aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat
     5821 aagaaacagg accctcttca aattggactt gtccgttcag cagctcaatg atggattctt
     5881 taaagtggtc agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt
     5941 ggatttcttt tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg
     6001 gatacgtgat catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag
     6061 acaatgcttg agagacagca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg
     6121 ttcctccaaa atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga
     6181 cttccaacat cggtacgcca gccgttccac ttggttctcc atcatcgctg cttcgctgga
     6241 tttcattttt ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt
     6301 tttttatttc ttgaatgaaa ctttttgctt ggtcctctga ttctattcgt ctaagagaac
     6361 agataaagcg tgattttttg atctcaatct cactttgtcc attttctttt attgtacgat
     6421 agcgctcgat catcttgtca cctctttttt tcattttctt tattcagtat aaataaagtt
     6481 ctaggttgtt tcaacactgt aacggttgcg caatcgctgt agtatgcgat aataaagaca
     6541 aaggtaggtg gatctgggga tgcaggaacg aatgaaaaag tacgatgcaa ttacgcatta
     6601 tttgaagaac aatggcggct ctcaagtaac gttgaccttt acccaatttg acgaactgct
     6661 gtttcccagt aacggacttc ccaaaacagc aagggaaagt acagattggt gggcaaatga
     6721 ttataaacac cctgaaaaag gtgcctatgg ctggataaat gcaggctatg aagtagttgt
     6781 catcaatttg gacaaagaat atgtcgtgtt caataaatta gtgaaatcta gttggctatt
     6841 tgattaaata aaccgcgttt agaaaacgtt gacaaatttc tttcttttta tataatagag
     6901 taacagacgc gaagaaaacg tttgcgtagt ttgttttaaa aaggagagaa aagtatggct
     6961 aaaaaaacaa tcatgcttgt atgttcggct ggtatgagca caagtttact agtaacaaaa
     7021 atgcaaaaag ctgctgaagc aaaaggaatc gactcggata ttttcgctgt gtccgcttct
     7081 gacgcagata acaatcttgc aaataaagat gtagatgtac tgttattagg acctcaagtt
     7141 cgctttatga aagctgattt tgaaaaacgt ctggaaccaa aaggcattcc tttagacgtc
     7201 atcaatatgg ctgattatgg tatgatgaat ggcgaaaaag tgttagatca agcaatcacg
     7261 ctcataggtg aataaataga acagtataac tatcgaacaa gcgatccaaa aaatagatcg
     7321 cttgtttttt cgtatatttt tttagggcga ataatatgaa tcatttttgg ggtagaaggc
     7381 ttctgacgag agatattaaa gcaatagact gtgaggaggt aaatttacca aattatgaga
     7441 agatcgcagc agtggaacgt ttgggcaact ggattctttg tcatcgctgt ggaatgagga
     7501 cgtccgtcat tgatggacag cttgcagatt acggttattt ttgtacacat tgtctatatt
     7561 tagggcgatg cgacactcaa caagatttat atttgtttga tcagcctgaa gcaaaacctc
     7621 gagaagtcgt ctttttttgg acaggacagc ttactgaaaa acaaacggaa atcgcaaaga
     7681 gaatactttg ccattcagaa aaaagacatc atcttatttg ggctgtaaca ggtgcaggga
     7741 aaacagagat gctttatcct attttagtgg aaactttaaa agctggagga agagtagcga
     7801 tttgtacacc gcgagtcgat gtttgtaatg aattgttttt acgctatcgt tatgtttttc
     7861 cagaagaaaa aatcacgttg ctccatggga atacagcaac ggcttatacc tttacttctt
     7921 ttgttatctg tacgattcat cagctatacc gtttttatcg gtcctttgat ttaatagtga
     7981 tcgatgagat cgatgcgttt ccttataacg gggatgcagg attagctcat gctgtgcaaa
     8041 cagcgattaa accagacgga cgattcatct acttaagtgc cacacctgat aaaaagttac
     8101 taaaggaaat caagcagacg tttgacatcc acaaacttgc tttgcgcttt catcggcgtt
     8161 tacttcctga acccgtcttg gttttcatga ataattggcg acaaaaatgc aagaacaaga
     8221 agaagataaa atctttggtg cggatcattc atcaattaat taaagaaaac catatccttt
     8281 tattttgtcc aagtatcacg atgatgcatc gtttgaaagc cacacttcaa gaggctcttc
     8341 cgagatatga gatcacagat gtttcttcaa gagacaatca gcgggcggaa aaagtccaga
     8401 aaatgcgaga agcaaaatat gacatactgt taaccacgat gatccttgaa cgtggagtca
     8461 catttgaaag aatatccgtt atcgttttag gagcggatca tccggttttt actaaatctt
     8521 ctttggtaca aatcgcagga agagccgatc gtaaagggcc ttatacgaac agtcaagtat
     8581 actttttcta tgaagaaaaa actgcagcta ttcgaaaagc ttgtcaggaa atcaaagaaa
     8641 tgaatgaaga agggaagaag atgctgtgag gtgcagttgc tgcagtaaag atatcgtcca
     8701 aaatttaaca ttcagcgaga ttattggtgt ttcgaaatac agattacctc agacttgttc
     8761 agtttgtcgc gcacaatttc atttattgga aacaagaggc tgttctggct gtcaaaaacc
     8821 aatccatttc aatctagaga gtacgaaaaa gaaagtaaag tctattcccg tttactgcag
     8881 tgactgtcaa gaatggaaga agaaatatcc gacctatgat tttcagcacc gtgccttttt
     8941 tgtgtacgat gaaaatatga aggcctggct caatcaatac aagtttatgg gagatatccg
     9001 actagcaggc acctttgcat ataaatggac gcagataaaa aaagaatacc cgacatacat
     9061 ctattgtcca atacctttat ctgaaaaaag gcgaacagaa agaggattca atcaagtaag
     9121 cgagatgttg aaagcagcaa gaattccttt tcagatgcta ctcaaaagag agaagcatct
     9181 gctggctcaa gcaaaaaaca cgcgaaagga aagattggag atgcctcaac cttttgaact
     9241 tgcagtcacc ttttctaaga tacaaaataa aaaaatattg attattgatg atgtctatac
     9301 aacaggacag actcttttcc acgcagcaga ttgtttgcag gtcgcacagc cgaaaatcat
     9361 ccgaaccatt tctttagcaa gatgaattag atgtggaaat ttgatagcga tttaatttgc
     9421 aaataaccta gagttcgata taatagaatt aagaagaggg aaagagtggt ccttctcctt
     9481 cttgattgtg gtagacgaaa ggggtaattt ttatgtttag atataatgta cgcggcgaaa
     9541 atatcgaagt aactgaagcg attcgcgact acgttgagaa aaaagtaggc aaactagaac
     9601 gctatttcag tgacgcaccc gatgcaacgg cgcatgtgaa cttaaaagtt tatacagaaa
     9661 aaacagcaaa agtggaagta acgatcccat taccttatct tgtattacgt gccgaagaaa
     9721 cttctccaga tttatacgca agtatcgatt tggttgttga caaattggaa cgccaaatcc
     9781 gcaaattcaa aacaaaaatt aatcgtaaat cccgtgaaac gggcatgaat actgcagatg
     9841 ctactgcttt attcaatgaa gaggacgaaa acgaaaatga ttctgaatta gatatcgtac
     9901 gtacgaaacg cctttcattg aaaccaatgg atagtgaaga agcagtctta caaatgaaca
     9961 tgctgggaca taacttcttt atttttgaag atgcagaaac aaatggtaca agtatcgttt
    10021 accgccgtaa agatggaaaa tacggactga ttgaaacaga ctgatcagcc ttacttaaat
    10081 aacaacgaaa acttgcagca gctactgaca gctgccgcaa gtttttttga gtttcctcct
    10141 cttttagatg aaatatgaat aaattatgaa gttcttactt taatacgctt aagatatttc
    10201 aattatctat aactaatgat aaaatgaaaa gtaaagtcta catagtctgg ataatacaca
    10261 gaaggaaaag gagattacac aacgcatggc caatttgatt cgttcactta tcgaaaacga
    10321 taagaaagaa ctaaaacgtt tagatagcat tgcaaaaaaa gttgaagcat acgctgacca
    10381 aatggcagca ttaactgatg aacaattaca ggcaaaaact gctgaattca agggcagata
    10441 ccaaaaagga gaaacattag atcaattact tccggaagct tttgcagtcg tccgagaagc
    10501 agcaaaacgt gtattggggt tatatccaca ccacgtacaa ttgatgggtg gaatcgtgct
    10561 tcacgatgga aatatccctg aaatgcgtac tggtgaaggt aaaaccttga ccgcaacgat
    10621 gccagtatat ctaaatgctc tgactggcga aggggtacac gtagttaccg tcaatgaata
    10681 tttagcaacc cgtgactcga cggaaatggg cgaactttat aatttcttag gattaacagt
    10741 ggggttgaat atcaactcaa aatcttcgga tgaaaaacgc gaggcttata attgtgatat
    10801 cacttatagt acgaataatg aattaggatt tgactacttg agagacaata tggtcgttta
    10861 ccgccatcaa atggtacaac gtccgctgaa ttatgccatt gtcgatgaag ttgactcaat
    10921 cttgattgat gaagcgagaa cgccgctgat catttcaggg caagcagaaa aatccacagc
    10981 tttatataca agagcagaca acttcgtgaa acgtttgaaa gaagaagaag attataaaat
    11041 cgatgtacaa tcaaaaacaa tcagtttaac cgaagcaggg atcgaaaaag cagaagaaac
    11101 attcggtttg gataacttat acgacattga aaatactgca ttgacccatc acttggatca
    11161 agcattgaga gccaactata tcatgctttt agatatcgat tatgttgtcc aagacggaaa
    11221 agtactgatt gttgaccagt tcactggtcg gatcatggat ggacgccgtt attcagatgg
    11281 acttcaccaa gcaatcgaag ctaaagaagg cgtagagatc gaagacgaaa cgaaaaccat
    11341 ggcgacgatc actttccaga actatttccg tatgtacaag aaacttgcgg gaatgactgg
    11401 tacagctaaa acagaagaag aagaattcag agaaatttat aacatccaag tataccagat
    11461 tccgacaaat cgtccggtta tccgtgatga tcgtccagat ttgctttacc caacattaca
    11521 aagcaagttc cacgctgtcg tgcaagatat caaagatcgc taccgcaaag gtcaacctgt
    11581 gttagtcgga actgttgcgg ttgaaacatc tgagttatta tcagatatgc tgaatcaaga
    11641 acgtattcct catgaagtgc tgaatgcgaa aaatcacttt aaagaagcag agatcatcat
    11701 gaatgctggt caaaaaggtg ctgtgacgat tgctaccaac atggctggtc ggggaacaga
    11761 tatcaagtta ggacttgggg taagagaact tggtggtcta gcagttatcg ggacagaacg
    11821 acatgaatct cgtcgtatcg ataaccagct tcgaggacgt gcaggacgtc aaggagaccc
    11881 tggtatgtca cagttctact tgtcattgga agatgatttg atgaaacgat tcggttcaga
    11941 acgaatcaaa tctttcttag atcggatgaa gatcggtgag gaagatgctg ttatccaaag
    12001 caagatgctg acgaaacaag tagaatctgc acaaaaacgt gtagaaggaa ataactatga
    12061 cacacgtaaa aacgtcttac aatacgatga tgtgatgcgt gaacaacgtg aagtaatcta
    12121 taaacagcga caagaagtaa tcatggaaga aaaaagctta tccaagccac taatgaacat
    12181 ggtcaaacgg acaatcagtc gtgtagtaga tgcgcataca caattagaga aagaaaattg
    12241 gaatttggaa agtatcatcg attttgccgg cagtactctt gtccatgaag atagtattac
    12301 tcttgcagat atcgaaggaa aaacagctca ggagatcaaa gagtatctaa acgaacgagc
    12361 acaagaagta tttaatacca aagcggctca actaaatggt ccagagcaac tgttggaatt
    12421 tgaaaaagtc gtgatcttgc gtgttgtgga ctctaaatgg acagaccata ttgatgcaat
    12481 ggatcaatta cgtcagtcaa tcggcttacg tgcttatgga cagaacaatc cattagtcga
    12541 atatcaaaca gaaggctata agatgtttga agatatggta ggcgccatcg agtatgaagt
    12601 cactcgaatc tttatgaaag ctgagatccg tcaaaacgta cagcgtgaac aggtttctca
    12661 aggaacagct tctcactcag aagacggaga tgccaaagaa aatagtaata ctagcgcgaa
    12721 aaaacagcca atccatgtga acaaggtcgg aagaaatgat ccttgtcctt gtggaagcgg
    12781 gaaaaaatat aagaactgtc atggaaaaaa tgcaaactaa tttctgacag caggattggt
    12841 gagacaaatt ctttttttaa gaattcgtct caccttcttt ctgttatttg ctgattctgt
    12901 tagactagat ataatgaaaa aagtatagtg aaagaaggaa aaaacatgga aatatccgaa
    12961 attcgaaatc tcctgacaga aatgcaggaa aaggtcacaa gcttcaggag gtctctttga
    13021 cttagaccag ctggaagaag acattgcgga agcagaagca aaaatggctg aacctggttt
    13081 ttgggatgat agcgaagctg ctcaaaaagt gatcagtgaa aacaatgcaa ataaagaaac
    13141 ttatgatcaa ttccattctc ttgctgaaga actggaagaa atcgaattga tggtggagat
    13201 gttgcaggaa gaacctgatg cagccatgca agaagaagca gaaacacgta tcaatgcgct
    13261 agatgaaaaa atgaagacat atgaattatc tatgttgtta gatggtcctt atgatcgaaa
    13321 caacgccatc gttgaattgc atccaggggc tggcggaacg gagtcccaag attggggcag
    13381 tatgctttta cgtatgtata cacgctgggc agagcagcat gggtatcaag tagagacact
    13441 agattatcaa gcaggtgatg aagcaggaat caagagtgtg accctactga tcaaaggaca
    13501 caacgcattt ggttatctga aatcagaaaa aggtgttcat cgccttgtac gtatctcgcc
    13561 ttttgactca gcgaaacgac gtcatacttc tttttgttcg gtagatgtga tgccagagct
    13621 tgatgaaacg atcgatatag aaatcaatcc agatgatttg aaaatcgata cgtaccgagc
    13681 aagtggtgca ggtggtcagc acatcaataa aacagaatct gctgtacgga tcactcactt
    13741 gcctacagga acagtagtag ctagtcaggc acaacgttca caattgaaaa accgtgagca
    13801 agcgatgggg atgttaaaag ctaagcttta tcaattagaa gtggaaaaga aagaacaaga
    13861 agcagctgca cttcgaggag aacaaaaaga aatcggctgg ggttcacaga tccgttctta
    13921 tgttttccat ccatattcta tggtaaaaga ccatcggaca aacgtagaga caggaaatgt
    13981 ccaagcagtt atggacggtg accttgattt atttattgac ggctatttaa aaatgcatct
    14041 acaataagaa cccattgtta aattgaaata aaattgtaag attttgcaag catgttgaaa
    14101 cattgccatg ctataatagc taaagactga aaaagatatg gagagaacgc catgattgaa
    14161 atgaaggatg taatgaagaa gtactcaaat ggtacgaccg ctatccgcaa tctctcaatt
    14221 gtcatcgaac aaggagagtt tgtctatgtg gttggtccgt ctggagccgg taaatctaca
    14281 ttcatcaaat tgatgtatcg agaagaaaaa gcttcaaaag gaactttgaa cgtcgcaggt
    14341 catgatctaa tagcaatcaa aaaccgcgaa gttccttatc tacgcagaga aatcggcgtg
    14401 gtgttccaag actataaatt attaccaaga aaaacagtat atgaaaatgt tgcgtacgct
    14461 atgcaagtta tcggcagaaa acctcgggat atcaaaaaac gagtaatgga agtgctggac
    14521 cttgttggtt tgaaacacaa agttcgagtc tttcctagcg aattatccgg tggggaacag
    14581 caacgtgtct ctattgctcg ggcaatagtg aatacgccta aagtacttat cgcggatgaa
    14641 ccgacaggaa atctggatcc agaaaactca tgggaaatca tgaaattatt agatcggatc
    14701 aatgcccaag gaactactgt cgtgatggct acacataaca gtacgatcgt aaatacgatt
    14761 cggcaccgag taattgctat cgaaaacggc cgcattattc gagatcaagc ggaaggggaa
    14821 tatggatacg atgattagaa cctttttccg ccatttatta gaaagtatca agagtttgaa
    14881 acgtaacggc tggatgacag ttgcttcagt cagtgcggta acgatcacat tgactttagt
    14941 cggtgtattc atggcagtta tcatgaacgc aactaaatta gctcaagata tagaaggaaa
    15001 cgttgatgtc tctgtattcg tagatatcgg tacaaaagac gaagagatga aaaagctaga
    15061 aaaagaattg aatgacttag atcatgttaa aaaagttgaa ttttcaagca aagaacagga
    15121 attgaaaaaa atccaaaacg aaatgggtga ttcctggaaa ttgtttgaag gtgacaataa
    15181 cccgttatat gatgtgtatg tagtaagtgc tactgatcct tcttatacaa aacaagtagc
    15241 agaagaagca gcagatttga agaatgtttc tcgttctgat tacggtggag cttcctcaga
    15301 tcggattttc caaatcgctg gtgcagttcg gacatgggga ttagttgcag cggcattgct
    15361 gctgttcgta gcgatgtttc tcatttcgaa cacgattcgg atcacgattt tgtctagaca
    15421 gcgtgaaatt caaatcatgc gtttagtcgg tgcaaaaaat ggatatattc gctggccgtt
    15481 tttccttgaa ggtggctgga tcggtctgat cggtgcgatt cttccgattt tgatcatcac
    15541 atttggttat gcatggtttt tccaattgat caatccatca ttattacgtt cccattattc
    15601 attgattcat ccacaaaata tcgtttggaa aatcaatctt ttaatggtcg gcattggtgt
    15661 gatcattggt tcacttggct cgatcatttc tatgagacgt ttcttgaaat tctaatacca
    15721 caatataaat aaaatgagaa agttggacag tttcgttcca gctttctttt tttattggct
    15781 aggagaatag aaggaagcgg cttttttccc tttactttaa aaaacaactt gggtattctt
    15841 aagttggtag aagaagaggt gaagccgtga aaattatcgt tgacaatttt tttacgatcc
    15901 ttttgattat gaatattttg ctttccttta tcatcgtttt tcgagaaaga aaggaaactg
    15961 ctcaaacatg ggcatgggtg ctcgttttga tgttcattcc agttgtagga tttattttat
    16021 atattttttt aggaagaggg atttccaaag agaaaatctt tgatttgaag atccaaggga
    16081 aaatcggtaa gaacctggaa attgaagaag acaaacaggc attgatgcga gggctttatc
    16141 ctcatccgcc tactggacaa gtagatgtga aacaattgat ctatatgctc acggtatttg
    16201 aaagtacgct ctatacgact aaaaatgaaa tcactttatt tacagatggt cgtgaaaaat
    16261 tcgatgcgct gattgaagat atcaagcagg cagaagacca tatccacttc cagtattata
    16321 tttatcgcag tgatgcatta ggtgaagaag ttcgagatgc attgacagag gcggcaagac
    16381 gaggggtaaa agtcagagtc cttcttgatg catggggttc cacacaagtt tcttccagct
    16441 tttttcagaa tttgaaaaaa gcaggcggag aagtcgcatt tttctttcct ctatttgttc
    16501 cgtatattaa tcctcggatc aattatcgca atcatcgtaa aatcgtagtt attgatggga
    16561 aaatcggata tacaggtggg ttcaatgttg gaaatgaata tctaggattg gtaaagaaat
    16621 ttggttattg gcgagacaat catttgcgta tttacggaga agcggtgtat accttgcaaa
    16681 accgtttttt gatggactgg aattctcagc atacaaaaga agtaggctat tctcccaaat
    16741 tttttccgtc cattcactct actggtgata tagctgttca aatcgtgaca agcggaccag
    16801 ataccgaaca tgaacaaatc aaaatgactt atttgaaaat gatttcgtta gccaaacggg
    16861 agatattgat ccaaacgcct tattacatac cggatggatc gattcatgaa gcactgaagt
    16921 tggctcttct ttcaggcgta aaagttcata tccaaatacc taacaaacca gatcatcttc
    16981 tcgtttactg ggcaacgtat tctttcgctg cagagctaat cgagtatgga gcaaggatcg
    17041 aaacatatga gaatggattt attcatgcaa agacgatgat tatcgatggt ggcattgttt
    17101 cagttggctc agcaaatatc gatgttcgtt catttcgctt ggactttgag gtcaatacac
    17161 tggtttatga tgaacgaatg gcttcacgtg taagaaaagc tttttttgaa gactcgaaaa
    17221 tttctacgca tctcacaaaa gaaatgtatg aaaaccgagg aataatcatc aagatgaaag
    17281 aaggattagc aagattgatt tcgccgctcc tttaaaacat aaaaaggaca tgagaaccat
    17341 caacagatgg agatctcgtg tccttttcta acggggccat ttctgagagt acgtcagaaa
    17401 tggagaaagc gtctcgcatg agcgaaatta
//
LOCUS       DAPCMU010000051        17313 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPCMU010000051 DAPCMU010000000
VERSION     DAPCMU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.052"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            10..447
                     /locus_tag="RD744_001382"
     CDS             10..447
                     /locus_tag="RD744_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557387.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            542..1426
                     /locus_tag="RD744_001383"
     CDS             542..1426
                     /locus_tag="RD744_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDU0557388.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1650..4817
                     /locus_tag="RD744_001384"
     CDS             1650..4817
                     /locus_tag="RD744_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557389.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4837..5382
                     /locus_tag="RD744_001385"
     CDS             4837..5382
                     /locus_tag="RD744_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HDU0557390.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5410..5865
                     /locus_tag="RD744_001386"
     CDS             5410..5865
                     /locus_tag="RD744_001386"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HDU0557391.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5862..6620
                     /locus_tag="RD744_001387"
     CDS             5862..6620
                     /locus_tag="RD744_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDU0557392.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6706..7446)
                     /locus_tag="RD744_001388"
     CDS             complement(6706..7446)
                     /locus_tag="RD744_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557393.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7507..8760
                     /locus_tag="RD744_001389"
     CDS             7507..8760
                     /locus_tag="RD744_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDU0557394.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9082..9309
                     /locus_tag="RD744_001390"
     CDS             9082..9309
                     /locus_tag="RD744_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDU0557395.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9432..9758
                     /locus_tag="RD744_001391"
     CDS             9432..9758
                     /locus_tag="RD744_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDU0557396.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9826..10539
                     /locus_tag="RD744_001392"
     CDS             9826..10539
                     /locus_tag="RD744_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDU0557397.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10849..11700
                     /locus_tag="RD744_001393"
     CDS             10849..11700
                     /locus_tag="RD744_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0557398.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11821..13686
                     /locus_tag="RD744_001394"
     CDS             11821..13686
                     /locus_tag="RD744_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0557399.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13734..15143
                     /locus_tag="RD744_001395"
     CDS             13734..15143
                     /locus_tag="RD744_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDU0557400.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15251..15952
                     /locus_tag="RD744_001396"
     CDS             15251..15952
                     /locus_tag="RD744_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0557401.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16111..16590
                     /locus_tag="RD744_001397"
                     /pseudo
     CDS             16111..16590
                     /locus_tag="RD744_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5775 a         2905 c         3721 g         4912 t
ORIGIN      
        1 agtagataaa tggagtggtt ttgcaaattt cttttatttc tagaagttat tcacgtatac
       61 ggatttgttt ttctcacaaa taaaaaggca ggaagtacaa tggatcattt gtatataaaa
      121 tatattcatc aattttccgt tttggtgtta ggcacactaa ttatagcggg gattgtcgca
      181 tttattattc aaggccgtga catattaaca gtacatcaag agtttttcag ttatgcattt
      241 ggtattcctc tgatcgctat aaggtctgat aacaaaaagt ggaagaaacg ctattacatt
      301 ctcttgctat ttttgtgttt ttatctgcca cctaagattt ttactttatt aggtatacaa
      361 tgcctatcat tttcttttat ttcctatatc attgttttaa ttttcctcag tttcatagta
      421 tggatgtttt atcttgtcaa aaaataaatg aatgtttcat gaacagtact tcatcacagc
      481 ctaatttttt tctttatgct atcgttatat taaagaaatc aatcagaagg gaaagaaagt
      541 gatgaaaaaa gttttattag tggtaaaccc cagttcaggc ggtgaacaag caaaggaatt
      601 tgagcaatta gcgatagcaa aattggaatc ggtgtttgat gaagtggtcg tcttgcatac
      661 aaaaaaagca ggggatgcaa aaaactttac tcgcgaagcg gctacggaag ggtatcatag
      721 tgtgtttgtc atgggcggag atggaacagt caacgaagga atcagtggaa tcgctgaaca
      781 agaacatcgg ccaaatttcg gatttttccc attgggtacg gtgaatgact tggctcgagc
      841 attagggata ccgctagaac ctgaggaagc gatcaatcat ttttcgattg aatcagtcaa
      901 gccattggat attggaaaaa taaatgacga ctattttatg aatgtcgtcg caattggctc
      961 tatccctgaa tcaatcaatg atgtagatgc ggagaagaag acgaaatttg gaaaactagc
     1021 ttatttcatg tcaggaatca aacagttagc ttccacacaa tcctactcgt ttcacgtaga
     1081 agttgatgga aagaaggaag aaattgagag cagtaccttg ctgataggac taactaactc
     1141 agtcggcggt tttgaaacat tacttccaaa tgcaaaagtg gatgatggta agctccattt
     1201 ggtttattta aaagattctt ctttgatcga tacgcttaaa acattgccag acctattaaa
     1261 aggagtagat gaatctacga aaaatctagc ctaccttact tgtgagcaat tagttgtgtc
     1321 attagctgat gaggaagaat tgacgacgaa tgttgatgga gatgaagggg ataaattacc
     1381 tgtgacaatc aaagtattgc cctcacattt acaggtttat tgttaaaatg gaggttgtga
     1441 ccgaggagtt ggcgggagaa caccgcttgt tcgggataag ggactgtgac aagacttttg
     1501 tcacagcctt ttttttgtac aaaaaagaaa tttacaccta atagaaaaat ttttccataa
     1561 aaatacatat tttaaatatg aaagtacaat tggaaataga ttaaaacgat aaaataaaat
     1621 tgtcaaaacg tcagagagga tggtggagaa tgaatcaatg tgtaaaaaag ctgattgcgt
     1681 cgattttgtt tgtcttccct ctcttgatgg tgacatccgt ttttgccgat gtagagaaaa
     1741 caaacgaatc aacgcaatca gagaatcaaa gttttgacaa gactactttt tcagaagaat
     1801 cgattacttc tgaaactaca gaatctcaaa gtgaggcttc tactaataaa aggcaagaag
     1861 aaaataagac aagtgagacc tcagaagtag ctgaacaaac gtctgatagc agtgaagcgg
     1921 cactggcaag agctgtttca gcggatgggt acagcgaagc tgccacaaca gaagaactgg
     1981 cgcagcttct cgcagacgaa tcagtaacta agatccgact gattcagcct ttgactttag
     2041 acagggaatt agagatcaaa cgagatatcg ttattgattt tggtggattc gctcacaatt
     2101 tcggcacaca ccatatttat atcaatgaga tacctcagtc gatcgagttc cagaatttta
     2161 aaggaacagc tgcacatcca ggtggattga caccaaatat cgatggaaat gccattatca
     2221 ttgcttatat gaccgatttt tggggaagca gatatcattt tacgggagaa ataaaattta
     2281 ccgggacact tgatttgtat gatgggtcca aattaggact gatttacgct cctcgtgcaa
     2341 ctgtcacatt agatggtgtt tccggtgtgt tggatgtcca accagtgaag gaaggactga
     2401 atgccgctcc gggtaaagcc tatttttgta gaagctatca attgaatgtg atcaatggta
     2461 gtcaacttta tggaccgtat ctagggaaat tctacggttt tctagattca ggagatgaga
     2521 ctggtagtgg aaaaacagcg ccaggaatcc atatcttaag cggttcaaag gtctctcttg
     2581 attatgatcg ggcagatggc gttagcgatg aaggagaggc agtcgatacg ctacctggta
     2641 atgtcatgtt tgaagtcagt ggttcgggaa gcgaattttc ggtaaataca aagatcaata
     2701 ttaccaatga caacaatcgc ggaatcatcc aaatgagagg ttcaggatcg aaagtcactg
     2761 tatcgaatgg tgggaaaatc accatcaata cagcaactac gggcggattt cgcttgcaag
     2821 gagattctag tcaaatccat gtatcaagtg gtggacaaat ctcggtcaaa atggctggag
     2881 atgacgatca acccgggaac aatgggatgc gttttgtagg aaaaggcctt tctttgatcg
     2941 tagaaggagc cggatcagtt atcgacatcg ataagcagag tgggcgttcc agcgcagttc
     3001 gttttgagaa tggtacgcaa aagctgacag taaccaatgg aggctcattg agggtaagaa
     3061 atacaggtga tggcaaatct tacgtgaaca gactcaatca aggaaatcaa gcgatccaat
     3121 tttatgacgc ttctggtttt ctagaaagtc caggttcagc tgattttaca gtcactggag
     3181 aaaaatcaaa tatcgatata cgcgcagatt ccggagcaac tgtagataca actggagata
     3241 ttgatttaaa ttttacggca ggtgaaaaaa cttatttagt catggtcggg aaaacagggg
     3301 aagataagac cggcatcatc agtggggatg tgttgaatgt ggacttagaa tctcctacct
     3361 attttgattt ccaaaacaaa cggattggaa gcgagaccaa tggcataggc ggctgggtat
     3421 tccaaggaaa tggagattct tctttaaaga tcgcccattc agaaatcgct ttatggcgaa
     3481 aaacaggcgc aacattcgat aactttgaaa aagacccgga ttatttttct gatatgacag
     3541 acttatcgat caatgggacc gacttaggca atttcgtatc aagccctagt gcaggtttaa
     3601 caaatgaatt tgctcagaca ggtgccggaa tgaaaaatta taatcgaatc agtgccaata
     3661 atcagctccc gatgattgaa tccttaagag tgccaacgaa tgcagataaa aaaatctatg
     3721 ggcatgtcgt tgttcccgaa ggtgtcgagg caaagccaag agatgcttgg acaggcgaag
     3781 tagaagtgac tttacgtatt acttacgcag atacgacgaa atcatctgta gaaattactg
     3841 ctctcacgca aggacaaaca gatgaaaatg aaggggcagg atataaccca tggggagaag
     3901 gcgaacaagg cggcttgttc gaagttcaag tacccaatga tgagagatta aaaacggagg
     3961 atcaagtgca agttgttgct gctaaaaaag taagaggtgg ccaagaagtg acagaactgg
     4021 actctgccaa aacgacagtc gatgtgattc ctcctgaacc tgctaattta agtaccacat
     4081 tgatcaatgc agcttctcgc acgatcacag gtacaggtga agcaggagcc ttggtttcat
     4141 tgaaaaaaaa tcaagctgtt ttgtcagaaa caactattga tgatacaggg gaatttgttc
     4201 tttctatccc agaaaatcaa ctttttgcag gagatgtatt agaaattgta ttaaacgacc
     4261 aagcaggaga agctactgct aagggtgtgc agaacaagcc aagtaccaac gatgaaattg
     4321 ggaatcacaa tccaagtgat acgccagttg tttatcatga tgccccgcct tttgcacctg
     4381 ctacgaagat cgtagtagaa ggtggtctct catttcttgc cccttccaaa ctcgattttg
     4441 gtcagatcaa agcaactggt ttgacacaag aagcatttgg ccaaatggat atacaggaaa
     4501 aattatggat ttatgatcag cgagaaacga aaaatccgtg gatcttaacg ataaaattat
     4561 ctcagccttt cacaaatgat gcaggatttt ctatgcagga tgtcctatat ttcaagaaag
     4621 gagaggagta tcaattagtg aatgaagaag aactggaaat tgtcagagga caaaattcaa
     4681 cgaatgtgca agcagattat tcagaatatt taaataacgg tcgggcgttt aaacttgtct
     4741 taaccaaaga aaatcagata gctggtaatt actcagcaga gataacatgg acattagcag
     4801 atgcgccgac agaataaaga tcaaggagga tgattgatga atcgaatatg gaataaaagt
     4861 catcgaaaca actgctcttt acttatgatc cttctccttg gattcatcct ttggcagaca
     4921 cctgtccatg cctccgaatt caattttgcg gtagattttg aattaccggc taatcagatt
     4981 gataagaaca aaacatatat aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc
     5041 tacacggtct ttaaccgaac agataaatca gtaacagttg agacattgat ccactccgct
     5101 acgacgaatt caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca
     5161 ttaagttatg atatgaaaaa cttagcaaaa acagtgggag aaataactgt tcctcctcaa
     5221 agtgcggtca ctgagacact ttcactgaaa gcgccagaaa aagagctggc tggtgtggtc
     5281 gcaggaggga tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt
     5341 gtcgtcaatc agttttctca agtgaaagca atcttgattt gaaatagcga aaaagagtct
     5401 tccccagatt tgaaattaaa aaaagcttat gcggatcaaa gcgatggaag aaatgtgatt
     5461 aaggcgaatc ttcaaaatcc aaatgcttct tatatgttcg gcttgactac atcggttgaa
     5521 atctacaaaa aagaaacaaa agagccttac ctttcaataa aaaaagaaaa aatagatgta
     5581 gcacccaata caaattttga tttacttgtc ccattagaag gaaaaaaatt agctccggga
     5641 cattatcaag cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct ccataccgat
     5701 tttgaaataa ctgatgatgc agcggaccaa ttgaatgaaa aggatattga tttaaaaaat
     5761 cagccagaaa catttgactg gcgatggctg atacttgttg gagtcattct tattggctta
     5821 tttgctggat tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa acaattgcat
     5881 taagtaccgt tctagctgga agtctattat tatgtacaca aagtgttttt gctgagaata
     5941 agagctcagc cactacagaa ggaacgattt ctgtgaccaa accagatgct gcaaatcctg
     6001 aagacgttgt tccagttcct cctgttgatc caaatgatcc tgatactcct tttacacctg
     6061 acgaaccaaa tcagggaaca tccggattac tgacgattga ccaagctcca gatcttgatt
     6121 ttggaacaat caagttaggt acagcaaaaa cagtatatgc tgcattacaa agcggaacgg
     6181 acagtactgg agcacaaaca caagtacaga atttcgtaca agtaacggat aaaagcggaa
     6241 attatgccgg ttggaaatta tcagtcaaac gaacagaatt tattggtaca acatctgata
     6301 cgaataaact aactggttca aaggtgaaat tcaaaaacgc cgtgattcga aatagcgcaa
     6361 ctaatagcag tggaatgcca tctaccgttt ctggaactgg cgctgaagga atcgaaatcc
     6421 cagcagatca ggcagtagat ctagtaactg caggagcaaa tgaagggatt ggcacatgga
     6481 tttattcatt aggagagaat gtggaacaag ggagacaatc agtggaatta tttgttcctg
     6541 tatccaatta tgctgtagac aactacgttt caacattcac atggtcgtta acagatgctc
     6601 ctacagaaga ggaagcgtaa aatcatatca acgatttcag ggagtggagc cgattcaagg
     6661 cccactccct gaaatctgct atgcttacgt ctcagctctt attcatcaac tcgaagcagt
     6721 tttttggaag tcatggaaac gttcctttgt cttttcaaag atctttcgac caacgagtaa
     6781 ggagtcaccg ttgaaaaatt ctaacacata atgagcagaa tcaattttct gcaattgcga
     6841 taagttgata cagtaagatt ttaaaagtaa aaagtaagca tacttttcta actctgtctt
     6901 caattgtttt aaagaagtat tcactaattt atcttctgta gttgttttga ccataacttg
     6961 gtttttcaaa cctttgatac tttcaatata taaaatttcc cgaggataca ggacgagact
     7021 tccacctttt tgatcaataa agattttatc gacatttacg atctgctgtt ctttccaatt
     7081 gatctgttct agcacttgac gaatttgttc agataaattc ctgtcttttc tgatatatcc
     7141 aatcggaagg attttccgat tgatgatctc tactgcaaaa ttcgtataag cggtgaaaaa
     7201 aacaatatta ctatgtgcat tttttttacg tatttgttcg gcgacatcaa tacctgtgag
     7261 tgattgcttc aagtcgatat cgataaagaa aacatcgtga ttatcaatag tgcccaactg
     7321 gatcaactga ttgatttgag agatagaagt gagctgcacg atattctcga tatgatgatt
     7381 gaccaattct ctgatgatcc attcgctgtg aaactggtta tcttctatga taaaatactt
     7441 tttcatatgc aggctcctat taaaaaaata tgtgtacatt ttgaaacaaa acaatggagg
     7501 aataagatgg tcgcaacagt gacactttat ttgaccttta ctttgatttt agatttaggt
     7561 atctatttat tcttttcgaa acaactgcta gtaaaaaaca gctttggttg tatagctttg
     7621 atggtggtga cacttttact tcctatccct aaagtgagct ttccttacct agttggctta
     7681 ttattgatgt ttctttttta ttttactacg aaaaacctgt ttatcagctg tttgatcagt
     7741 aatctttttt ttgctatcac tggactcagc ttttttctgg tttttgatct gatggattat
     7801 ttaggaaaga gacatcttgg aacagcaagt ttttttgcac tacttatcgc atttattttc
     7861 tatctattag taaaaagtat agacaaacag acgagatttc tcaacttgct ttttacttat
     7921 ccaactgaac actattttgg aggtatttgt attttattaa gctggggcgt gatttacttg
     7981 gtattgggaa gtgttcactt ccgctcatct aattatctgc ttctatctct tttgtcgata
     8041 gtcatcagtt ttttttcttt gtttttttcc attatgttga tcagtttcca agtaaaagaa
     8101 agacaacatc aggaatcgct tcaactgtat caaataaatg aagaatatta ccatcatcta
     8161 gaggaattca aacatgatta taaaagtcta ctgttttcat taaaaattac tcttcaggaa
     8221 aataaaaaag aaacgttgga atttttagaa atattagaag atcattctgc agctattttt
     8281 gaaaaaccgc aaatacaaca gctaacaaat attgtatccc cagctgtcag gggtgtcttt
     8341 ttaaaatttc tagaaaaagc agaatcggag aaaatcccga tccaagtagt tattcctgtc
     8401 gaagtgaaaa caatcccgat tgatctggtt gtttttattc gctgtttatc tatttttatt
     8461 tctaacgcat tcgatcatcg tgtagccgat cagagtccga ttacgatcaa tctgctggaa
     8521 gaaaaagagg gatcacgttt tagtatagcg aacaaggcaa atccaacgaa tttgacgcta
     8581 tcggagatgg tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg tctatacagt
     8641 gcgaaaaaaa tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag
     8701 aatcactatt tttttgtaga aatgtatctg gcatctagaa agagcaaatc agcctactga
     8761 cttactgtca aaggataagt ttgaggaaag tctacacgga gatagagaca acaacacagc
     8821 atattcaaaa gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt
     8881 agttaaacaa agtagttgac acttccgcta cttagtatta gtacgaaagt atacttcatg
     8941 atactaagta gataagaagt gaaaagcgga acccacgatc gtcaaaatgc taactgcttt
     9001 gcttacacca gataaaggga agagcaatat cgcttatttt aggcgtggag aagcaagcaa
     9061 atccctacga tcacaaaaaa aatgctgacc cagcagttaa gagagttaga acaagatgaa
     9121 agtatccatc gaaaagccta taaccaagtc ccgcctaaag tagagtactc gctcaccgaa
     9181 gaaggaaaat cattaagaga gattctagta gcgttgtctg tttgggaaga agaacgtatc
     9241 gaaaaacaaa aagcctcaag ggaagaagac acgctactgc atacacatgg tggctaccta
     9301 aattattgat tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag
     9361 catcatctat tttttatgta aaaaaatggt atgattaatc aagtaagaag tgattggaag
     9421 aaggcaagca aatgaatagt atggaaaaag tcaaccagtt tagagatgaa cgtaattggc
     9481 gacaatttca taacgaaaaa gatttggcta tctctatttc tttggaagca tctgaattgt
     9541 tagaactttt tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca
     9601 aagaagaact ggcagatgta ttgatctatt catacatgat ggcagacaac cttcacctgg
     9661 atctagatga gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa
     9721 aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa atgtgtcatt cggctgttgg
     9781 aaagagcaaa agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga
     9841 aaagcaaaaa gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct
     9901 ttgattttca atgaacagat caaggattat tttgtcagag agacaggaga taaatatgct
     9961 atagcgaatg taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac
    10021 tttgatgcgg tagaaccaat gacgacagaa ggggtcatga ggtcgcaaat gagaggaaca
    10081 gatctaccag tgattgctag tatcgcagtc ccttcagttt caatcaattt accggttttc
    10141 aaaggcttgg ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa
    10201 atgggaaaag gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgctgttt
    10261 acaccgctag aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg
    10321 tatacataca aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat
    10381 gaagtcgagg gaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg
    10441 cgactagttg tacaaggaga actggaaagt gttacatcga taaaagatgc gacagatgat
    10501 atgagatctg ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt
    10561 acagattaaa aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa
    10621 agagaaacgc aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca
    10681 gtttttttat tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt
    10741 ttgaataaga aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca
    10801 gtaggcgaat tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat
    10861 aaaattttga ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt
    10921 atgggaaaag gactagcttt tcaaaaaaag attggggacg agatttcgcc agaagcggtc
    10981 caaaaagaat ttgttttaag agattctttt gctaaaaacc aattccagca gctgatggct
    11041 gatattccgg cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa
    11101 ctaaaagttg aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg
    11161 atcacacgag caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa
    11221 ttttatccga aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg
    11281 ggtgtggaac tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa
    11341 caaggaaacg aagacatgca agtgacgatg accgctacca caatggtaag agatatactg
    11401 agtatcatca gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt
    11461 atcattactc atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag
    11521 caagatgaat ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc
    11581 gaacggatca atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag
    11641 atttatttaa cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa
    11701 ggattgttac tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt
    11761 gactgttttt ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca
    11821 atggataatc aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc
    11881 aaaagtttag tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca
    11941 gatacgcaaa agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag
    12001 tatcaagtag tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa
    12061 ggtcttgcag tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc
    12121 gatattatct ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg
    12181 aaaggattct tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat
    12241 caaatattgt ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt
    12301 acagcagcta aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt
    12361 gtgtatccag ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc
    12421 ccagtgattc tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta
    12481 tgggttcaaa gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg
    12541 attttagttc cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga
    12601 ccaatcggga cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt
    12661 agtccaatcg tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc
    12721 atgcattggg gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta
    12781 ttgatgccaa tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca
    12841 ttgcgaacaa aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta
    12901 tttggaatca ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt
    12961 gccgcttgta tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct
    13021 ttctccagca gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt
    13081 gtagagtcaa atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc
    13141 gtaggtacat tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa
    13201 catgcgaatg caggaaaacc gattacttca gcacggcata ccttaaaaag tcctttaacg
    13261 ggtaaagtac tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga
    13321 aaaggtatcg ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc
    13381 acgactattt ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt
    13441 ttgattcata tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc
    13501 aaacaagggg atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt
    13561 agagcagctg gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc
    13621 gatatacttg aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta
    13681 aaataaggag gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc
    13741 aactgctgcc aaatcaatta gaaggggctt atgatcgaga cggaaaagga ctatccgttg
    13801 cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact
    13861 ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat cattacgatc
    13921 gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga
    13981 ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag
    14041 aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga
    14101 tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa
    14161 aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag
    14221 tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc
    14281 aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata
    14341 atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc
    14401 aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc
    14461 aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac
    14521 gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa
    14581 aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg
    14641 gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag
    14701 aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc
    14761 agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac
    14821 cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga
    14881 ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag
    14941 tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatcgat cttgtcagtg
    15001 catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag
    15061 gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg
    15121 cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca
    15181 tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa
    15241 gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga
    15301 agatattttc tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga
    15361 agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga
    15421 tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa
    15481 tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg
    15541 gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt
    15601 tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat
    15661 ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa
    15721 agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga
    15781 tttctcggca gtatttttaa cagaagaaga acaaggaatt ctgaaaacag acgcacagat
    15841 cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga
    15901 ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa
    15961 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
    16021 ctcatatttt tcgtttggtt aggcttatta ccgcagatac acggctgttt ctggattacc
    16081 atttattctg gataaggggg tgtggcacga ttttcagaag gaatctatga accaatcact
    16141 gtattgattt tagcttcgat ggtaacccca ttcggcttga cgatcgctta tttcttaggg
    16201 aaaatcattc gtaaaaatat actcaatcgt caagaaatcg atacattgaa gaccgctttt
    16261 cctatgggga tctgtcagat cacagaggga tgtttcccta tcgtattgaa cgacttggca
    16321 cgcaatgtca tagcaacggg agtcggtggg acagtaggtg gcggattaag catgttttgg
    16381 ggcgcagata gccgtattcc ggcatcggga atgtttgcag tcgctacgat gaccagacca
    16441 tgggcattca ttggtgcatt gttagctggt tcttttgtca caggaataac cattttatta
    16501 ttgaaaaaac cagtagatcc gaatgcagaa atcatccaag aagaaaaaga ggaagaagac
    16561 atatcttggg atgatttgac aattagttag gagaggcaga atggattaca agaaaaaagt
    16621 acaatagatg taccttgaga caggaatggt atgaaagaat cagagttaga aagaatcgaa
    16681 tatacagatt ttggtttagg agcgatcgaa actgtccaga gcatcggcac cctccttgaa
    16741 atgacgatga aggaaaaatg agaacctttc gttgtcgaaa gggtctggta taccttatgt
    16801 cgaaggaaag gaaacaaaaa aactttcctg tactattcca aaaaggaaag aaagttatta
    16861 tacagtaaaa aaagaaatta ttcttcatgc tagagatcag tacacgttcg agcccaatat
    16921 caaacactag ttccaggcag gcgcaacagg tgcggttatc actgaatttt cttccagcag
    16981 tgacgattca agtgatattt ttacaaatcc agctatttat cgttaacaaa aagattttta
    17041 ggagaagctg atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg gcttttttat
    17101 ttagagaaac atcatttatc agtgaaacat aacacgagaa caaaatgatt ttttcgttaa
    17161 taaaagaaac aagtatttgt tgtttaaaat aatacacgtt attggcatat aggacgaaat
    17221 cccagtatct tctataagta agtcatttca tatcgatgaa aacacgttat ttatttttgt
    17281 tttatctata aaatggctga aaaatgattt ttt
//
LOCUS       DAPCMU010000052        16851 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPCMU010000052 DAPCMU010000000
VERSION     DAPCMU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.054"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            303..2069
                     /locus_tag="RD744_001398"
     CDS             303..2069
                     /locus_tag="RD744_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDU0557402.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2171..3430
                     /locus_tag="RD744_001399"
     CDS             2171..3430
                     /locus_tag="RD744_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0557403.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3577..4878
                     /locus_tag="RD744_001400"
     CDS             3577..4878
                     /locus_tag="RD744_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557404.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4881..5732
                     /locus_tag="RD744_001401"
     CDS             4881..5732
                     /locus_tag="RD744_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557405.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5745..6560
                     /locus_tag="RD744_001402"
     CDS             5745..6560
                     /locus_tag="RD744_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDU0557406.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6711..7877
                     /locus_tag="RD744_001403"
     CDS             6711..7877
                     /locus_tag="RD744_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0557407.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8107..8754
                     /locus_tag="RD744_001404"
     CDS             8107..8754
                     /locus_tag="RD744_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDU0557408.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8838..9782
                     /locus_tag="RD744_001405"
     CDS             8838..9782
                     /locus_tag="RD744_001405"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDU0557409.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9917..11368
                     /locus_tag="RD744_001406"
     CDS             9917..11368
                     /locus_tag="RD744_001406"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDU0557410.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11389..12225
                     /locus_tag="RD744_001407"
     CDS             11389..12225
                     /locus_tag="RD744_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDU0557411.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12225..12884
                     /locus_tag="RD744_001408"
     CDS             12225..12884
                     /locus_tag="RD744_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0557412.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            13010..13699
                     /locus_tag="RD744_001409"
     CDS             13010..13699
                     /locus_tag="RD744_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557413.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13705..14661
                     /locus_tag="RD744_001410"
     CDS             13705..14661
                     /locus_tag="RD744_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDU0557414.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14705..15217
                     /locus_tag="RD744_001411"
     CDS             14705..15217
                     /locus_tag="RD744_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557415.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15473..16021)
                     /locus_tag="RD744_001412"
     CDS             complement(15473..16021)
                     /locus_tag="RD744_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0557416.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16110..>16851)
                     /locus_tag="RD744_001413"
     CDS             complement(16110..>16851)
                     /locus_tag="RD744_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDU0557417.1"
                     /translation="EVFISIKKELPFLSEKRLFTMSVSAMSTVSMSIVGSYMALIDSK
                     YVITALVLNLFGGYILASIVNPYVLEEKEDELIIEENKEQTFFQMLGEYILDGFHVAI
                     TVAAMLIGFVALIAMINAIFHGIFGITFQEILGYIFAPLAFISGIPWKEAVDAGSIMA
                     TKLVTNEFVAMTELANGNFHFTERTTAIISVFLVSFANFSSIGIISGAMKGLNEEKGN
                     LVAKHGLKILLTATLVSFLSAIVTGLLV"
BASE COUNT         5506 a         2987 c         3406 g         4952 t
ORIGIN      
        1 ttaggctagt ctaaattatt atgtttcatt cttctagaat atacttctac gttaaaaact
       61 gttaaaaaac ttttacttta gaaaattgga gaaattgatc gcttaagata aattcaggac
      121 actaaatagt acaggaagta gattaaaact tttaaaagtt ttaagtctat ctatttggca
      181 agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc gataacattt ttgaaagcgt
      241 tgtcttttgt ttctctatca cttatagtga tgacgtcata ttaaaacaca aggaggaaat
      301 caatggatac agctgcaatt tatcatcgac cggaaagtga atatgcattt ctatatacga
      361 atacaagatt tcgtattcgg cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc
      421 tttgtggaga tccatatacc ataacaactg aaaaatggta tcaaaaacaa cgaccaatga
      481 aaaaatgtct aagtaccaat gttcatgatt attgggaaat tgaagtgacg agcgaaacaa
      541 ggcgcttaca gtatgcgttc catgttgttg gggaagataa cacggactgt ttttacggtg
      601 atcaaggaat ttttccttat cgagaaaatg tgattactga accaaatttt tattttcgaa
      661 taccttattt tcatcaaatc gatcgattca ctattccaga atgggtaaaa gaaacagtat
      721 ggtatcaaat ttttcccgaa agatttgcaa acggggacaa agcaaatgac cccgaacaca
      781 ctttaccttg gggaagtaaa gatccagacc gagaagactt cttcggggga gatttgcaag
      841 gtgtgaggga tcatttggat tacttgacgg accttggcgt caatggcatc tatttttgtc
      901 ccatatttaa agccacatcc aatcataaat acgatacgat tgattattat gaggtggatc
      961 ccgcttttgg cgataaaaaa cttttaaaaa acttgattga tgaagcacat aaaagaggga
     1021 tacgtatcat gttcgatgct gtattcaatc atatgggtgt ccattcacca cagtggcaag
     1081 acgttctcaa aaacggagaa aaatcgatct ataaagactg gttccacatc cgttttttcc
     1141 cagtagattc ttatcagatg actgacttac cagaaactgc tgaaaatatc ccatatgata
     1201 cttttgcttt tactccattc atgccgaaac taaatacagc aaatccagaa gtgcaaaaat
     1261 atttgctgga tatcgcaact tattggataa aagaattcga tattgatgga tggcgtcttg
     1321 atgttgctaa tgaagtcgat catcattttt ggaaaaaatt cagagaagca gttacagaaa
     1381 tcaaaccaga catctatatc cttggagaga tttggcattc ctctcaagct tggctgcaag
     1441 gggatgaatt ccatgcagtg atgaattatg cattcacaga ttcgattaaa gattattttg
     1501 caaaaaagaa aatcacagct tcacaaatgg tcagtggaat gaatcatcag caaatgcttt
     1561 acagagatca ggtgaacgaa ggaacattca acttattaga ttctcatgat actgccagga
     1621 tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc
     1681 ttcaaaaagg gtctccttgt atctattacg ggacagaaat agggatgact ggtgaagatg
     1741 acccggattg ccgcaaatgt atgatatggg agaaggagga acaagatcta gagctattcg
     1801 gttttataaa agaacttgtt tctctaagga aacagttgtc taagattatt tctgaaggtt
     1861 ctactcagtg gctaatagta tatgatcgtg aagataagct ctatttcaca cgggaactag
     1921 agggacagat catctatgtt tactttaatc agtcaaaaga accttgggta gtagaacagg
     1981 aaaatgaggt gattcttagt caaaattgtc agttgttaga agacgggaaa gcagagatac
     2041 gacaatacgg atttttgatc atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt
     2101 tgcaaatgaa gatttgtgcc cattataata agggtgtaac agcacgttga atacacagga
     2161 ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt ttagggttaa tctctttagg
     2221 aatcttaggc ggattagcgg cttgtggcaa tggtaactct tcagcagaag gggaagccga
     2281 tgaaggaaaa acgttaacag tttccgttga ttctggatat aaagattata taaacgaaat
     2341 aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt
     2401 cgatcagttg gaatcattag cgttagatgg gcctgctgga aagggacctg acgttatgat
     2461 ggcagcatat gaccggatcg gtgcgttagg acagcaagga catctggcgg aagtaaaact
     2521 gggaaatgaa gcagcttacg atgaaacaga caaagcccaa gtgacttatg atggaaaaat
     2581 ctacggagaa cctgcagtaa tcgagaccct tgttttgtac tataacaaag atttagtcga
     2641 tacagcacca tcaacgttta aagatttgga gaatctatcc aaagacagtc gttttgcttt
     2701 tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa tggactgact tttattattc
     2761 ctatggttta attgcaggtt acggtggcta tgtatttggt gatgatggca caaatccttc
     2821 agatatcggt ttgaataatg caggtgctgt agaaggaatc tcttatgcaa cagattggtt
     2881 caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt gcaggagact tcgccagcca
     2941 gcaattcatg tccaataaaa ctgcagcaat cattgacggt ccatggcaag cgcaaacata
     3001 caaagaaaac aatatcaatt atggtgtagc caaaattcct actctaaata atggacagcc
     3061 ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga
     3121 cctctctcaa aaatggcttg attatgtaac gaatcaggaa aatcaagaaa aattctttga
     3181 aatggtaaat gaaatccctg caaaccaaca agctagggaa acagcaaagg ctaaaaacga
     3241 tgaattaaca acagctgtga ttgaacaata tgaaacagct caagcgatgc caaacatccc
     3301 agaaatgggc gaagtttgga caggtgcaga aaacttgatg ttcgatgcag catcaggttc
     3361 aaaaacacca aaagaatccg cagatgaagc agtaaaaaca atcagtgaag ccatagaaca
     3421 aaaatactga ttaaaatgat aaccgtccat ttttataaaa aatggaacag gtgcttcaga
     3481 taaaagtggc aagagcgaga tggaaaatta tcttgctctt gttgccatta tcttaaaagc
     3541 agtttgcggt ttagcaagtt tagagggggc attcagatgc agcaagataa aacaagcaat
     3601 atcaacgaaa aaaaaatcac gctttgttca cttattccag ggatggggca gtttgtaaat
     3661 ggccaagctt ttaaaggtat cgtttttttc gccatattcg tcctattcat tggtcagatg
     3721 cttctgggcg gatttcatgc attggtagga ttggttactt tgggaagtat accaatggaa
     3781 gatcattcat tatttatttt gattgaaggc actttgcaat tcatcattac aatcattttc
     3841 ttgatttttt acggtttgaa tcttttagac gcttatcgag tagcgaagat gcgtcaagag
     3901 ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata atataggtga tgaaggtttt
     3961 ccttatctat tgacacttcc cgcttatctt ctcatgattt ttacgatcat tttcccagtc
     4021 ctagttacat tgttcactgc atttactaat tatgacttca atcatattcc accagccagt
     4081 ttaattgatt gggtaggggc taaaaacttt tttagtatct tctttttaag ctcatatcgt
     4141 aatacttttt ttgcagtctt ttcgtggacc gtgatttgga ctttatgtgc aacaactttg
     4201 caaatcaccc ttggtattct cacagcggtt gttgctcacc agaaatttat taaattcaaa
     4261 cgtattttcg gtatcatttt cttattacca tgggcggtcc cagcttttat tactatcatg
     4321 agtttttcga atatattcaa cgatagcgta ggtgccatca atactcaagt gattcctttc
     4381 ataaaccagc ttccttttgt agatatcagt gcaatctctt ggaaaacgga cccgaactgg
     4441 acgaaagtag caattatctt agtgcagggc tggttaggat ttccctatat ctatgtgatg
     4501 gttacaggaa tcttacaatc gatttcagaa gacttatatg aagcagcaaa aatcgacgga
     4561 gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg
     4621 ccaacattta ttacacagta tacggggaat ttcaataatt tctccatgat ctatctgttt
     4681 aacaatggtg gtcccggaag cgtaggcgga ggagctggtt ctactgatat tcttatttcc
     4741 tggatctata aacttacgac tggaacttct ccgcagtatt ctatggcttc agcaatcacg
     4801 ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg
     4861 ttcaatatgg gggattaaca atgaaaaaat ttcattcgag tgctcgattc caaaaacgac
     4921 tttctcactt ttttacttac ttttttcttt taatactgtc agtcattatc atctatccgc
     4981 ttttgatcac agcaagtact gccttcaagt caggaaatat tgcagctttt agtttagatc
     5041 tttcaagtga ctggacttta ggaaacttta ctcggctatt cagtgaaaca ctttacggga
     5101 cttggtataa aaatacattg atcatttctg tcctaacaat gatcgtacaa gtgactgtag
     5161 taactttggc gggctatact tatagtcgct atcgttttat cggccgcaaa tcaagtttga
     5221 tgttcttttt agtgattcag atggtaccga ctatggcagc attgactgcc ttttatgtca
     5281 tggctttctt attagaagcg ctcgataagt actggttctt gacaatgatc tatataggcg
     5341 gtggaattcc aatgaatact tggctgatga aaggatactt tgacactgta ccaatagatt
     5401 tagatgaatc tgctaaactt gatggggcag ggcatttcag gatttttttc cagattgtat
     5461 taccgcttgt caaaccgatg ctcgccgttc aagcattatg ggcttttatg ggaccttttg
     5521 gggatttcat gttagctaag ttcctgcttc gaacacctga aaatcagact gttgctgtcg
     5581 gcttacaaac atttatttct gatgtgacaa accaaagagt ctctcttttt gctgctgggg
     5641 caattttgat tgcactaccg atttgtctat tattcttctt tttacaaaag aactttgtta
     5701 ccggcctgac atctggcgga acaaaaggat aggagcagag tatcatgcaa acatttccgt
     5761 taaactattt ttccgcatta cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat
     5821 catggcctaa atttttcttg atatttgtat ttttagtttc cttgatggtc atgcctgtga
     5881 ctctttttta tgccaatcag atccaagcga ttccattaga acaatttcta tccgttcatt
     5941 cgctgatcga tgaacaagga acccaaaaat tttctgaact tgaattatct gaaactgggt
     6001 tacaagcatc gcaacaaacg atagctgtga cccctgaaat tttggtaggt gtgtctttat
     6061 ctgagaaaca acaatccgat catggaactt ttattgattt tgagaaggaa caatgggtga
     6121 tccaacaaaa ggataaatct ggtatacgcc gctatacgat gaattatagc ccatctttcc
     6181 aaccagattc tgtacgtacg cccgaagatt tccaacgatt tttagaaaga gaattttatg
     6241 ccagtaatcg tccaacaatc attttatctt atagtttaag tcttggtctt gtcctatttg
     6301 tcatgacgag tctcatttta ttcggggctt cctttttctt gtggatgaca agaaaaagcc
     6361 aactttcatc cattcataca ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa
     6421 taggttccat ggcagcagca gttgtagggt ttatccactt tgactttatc ttgatgctaa
     6481 gtgtccaaac aacgataact gtcttgctgc ttttatgggt ttttgtcaaa acaagattca
     6541 aagataagcg tgtcgaatga caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa
     6601 tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt aaacttctta gttgattctt
     6661 ttctttttta gtattaacgt aatagggtag tttttttaag gaggaaaatc atgttagaga
     6721 aaactttata caatcaatta ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt
     6781 gggacggtac aacgaaacaa tacggagata cggacactcc tccccaaatc aaaatcaata
     6841 tccatgaaga aatcccaatg aaagagatta caagtaacgc ttctttggca ctaggagaag
     6901 cctatatgga tcatcgaatc gagatcgagg gaagtatcca agcattgatc aacgatgctt
     6961 atcagcaagc aaacagcttt ttacgaggaa aagattatct aaaatggctg ccaaagaaag
     7021 aaaaacatac gaaacaaaaa aacaaagaag atatccatgc gcattatgac ttaggtaacg
     7081 acttttataa actttggcta gatccaactt tgacttattc ttgtgcttat ttcaatacac
     7141 ctgaagatac gttggaacaa gcacaaatca ataaagtcca tcatatctta gacaaactct
     7201 ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg ctggggaaca ttgatgttca
     7261 ctgctaccaa agagtataat gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg
     7321 atttgattac caaaagaata aaagaagaac atttagaaga taaatgccgt gtattattaa
     7381 tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg
     7441 aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac
     7501 caaaaggaac agctttgatc catggaatca gccgacaaca aggcggtgcg acaaatgctt
     7561 ggatcaataa atatattttc cctggcggtt atgtccctgg tgttactgaa ttagttgata
     7621 atattacaaa aaacgatttg caactgattg atttggaaag cttacgtcgt gactaccaat
     7681 tgacattaga acattggaca aagaattttc atcaagtcca agatcaagtc acgaaagaaa
     7741 tgggtgattc cttctatcga atgtgggatc tatatttaca agcttgtgca gcttcatttg
     7801 aatcaagtaa tatcgatgtg attcaatatt tgcttgttca acctggaaac aatgacatcc
     7861 caatgcatcg tgcataattg aggcaaaagt actgtttaaa gactatttaa aaagaagcta
     7921 agttttatga tgctccagaa gtgaactttt ttgtctaact tctaggattg tctctaaagc
     7981 ttagtttctt tttctttata ttttcataat aaaagtttgg ttctttaatt tttaaacatg
     8041 acagaaaatt ttttcttcgt tatacttgta atagaatact attttataac aaaggggatc
     8101 gaatcaatga aaaaaactgt tatttatact agtttatttg cagctttgtt tttagctgct
     8161 tgtcaatcaa ataccaatca aggcacatct gggaatacgt cttccagtag cgaacagcgc
     8221 tcttcacagg tatcggactc ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa
     8281 agttctagtg aagcaagtac tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa
     8341 ttgttccaag ataaataccc agatactgca attactagtt tggaattaga tagtgattgg
     8401 ggatcttact tctacaagat agaaggtgtc gatgatcaaa aagaatacga agcaagaatt
     8461 gatgctcaaa atgaaaaaat ggagacagag aatccagaaa ctctagatcg agatgaacaa
     8521 aatggtcaaa agaaaaaaga agatggtctg gatgttgata aattgatcac gattgaggaa
     8581 gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg caacagattg ggatttggat
     8641 aaagatttag gtactactta ttgggaagtc aaagtaaaag acggcaatca aagtacatca
     8701 gtcaagatca atagccaaac aggcgaagta ctgtcaactg aacaagatga ttaagaattt
     8761 cttcggaaaa caagagttga tttttctttt agtcatgata cactagacaa aataacttgt
     8821 ttgaaggagc gatttacttg agtttagaaa cgacctttgc caatcatatc tttgccaatc
     8881 ctttgatgaa tgcatctggt gtccactgca tgactaccca ggaattagat gaactggctc
     8941 attcagaagc aggagcattt atcactaaga gctgtacgat caatgaacga aaaggaaatc
     9001 ccgaaccacg ctattttgat gttcctttag ggagtatcaa ttctatggga cttccaaact
     9061 taggtttttc ttattatcta gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa
     9121 atcaaccgtt atttttttcc attgccggga tgagtgtcca ggaaaacttg gaaatgcttg
     9181 agaaaataga aaaaagtggg tttaatggga tcactgaatt aaatttatct tgtcccaatg
     9241 tgcctggaaa accacaacta gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag
     9301 tattcagcat cttttctaag ccgctaggaa tcaaattgcc tccttatttt gattttgctc
     9361 attttgatca gatggctgat atcttgaatc agtttccatt gacctatgtc aatgctatca
     9421 acagtgtggg caatggtttg tatatcgata cagaacaaga agcagtagtc atcaaaccaa
     9481 aagaagggtt cggcggcatt ggcggagagt atatcaagcc gactgctttg gcgaatgttc
     9541 gcgcttttta tacacgtttg aaaccagaaa tccaaatcat tggtacaggt gggattcgga
     9601 caggacaaga tgcatttgaa catctgctat gtggcgcaag catgttacag attggtacag
     9661 agcttcataa agaagggccg gaaatttttt ctagaatcat aaaagaatta acgcagatta
     9721 tgtcagaaaa aggatatacg tcaattgacg aatttaaagg caaattacgc accatttcat
     9781 agcaaagaga cgaaggtcat gattgatctt cgtctttttt tataaaaaac actcgtctgt
     9841 tcattcctgt gcaagcgtac tcattgtatg ttagacgaac ttgtgggaaa ataacagagg
     9901 caaaaaggag gataatatga atgatgcaac agaattagca aaagagctta aagaaaaaga
     9961 atttagtttt caagaactag ttacggatat acagaaaaaa ataaagaagc aaaatcatga
    10021 attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat tcccacgagc tattgattcc
    10081 ccagtcagaa aaagggccat tggctggtat cccatttcct ttaaaaatgc tcggtcaaga
    10141 aaaaaaggga tggctagcta cttcaggatc tcgtttattt gaaacacacc gagcttctca
    10201 tacatctaat tatgtgcggc aagcagaagc gataggtcta gttccttttg gacaaaccaa
    10261 tgcgccagag tttggtttca aaaatattac agacccagtc atctatggcc cagcaagaaa
    10321 cccttggaac cttgaatgta caccgggcgg atcaagtgga ggagctgctg ctgcagtttc
    10381 atcaggaatc gttcctttag ctggtgctag tgatggcggt ggttctatta gaatacccgc
    10441 ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg acgatgccag ttggtccata
    10501 tgcttggaga ggatggcaag gagctgctat tgatttcggc ttgacagttt ctatgagaga
    10561 cacggaggct ttattttatg ggatgagaag tatccattcc ggtgcgccat atcaagtatc
    10621 gccagttgag tggcaaacac atccaagaaa aaaacggttg aagatagctt tttgtgttga
    10681 ttcaccgatc cactcacaga ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga
    10741 tttccttgga aaagaagggc atgatttgac agagattcct tacccactag atggtcgtaa
    10801 actgatcacg tcttattatc agatgaatgg cgcagaaaca gcagcaatga tccgctcgat
    10861 tgaaacaggc atccagcgag ctgtcagaaa agaagaactt gaacccattt cttggacttt
    10921 gttccaatat ggtcagaagc tctctgccgc tacttacatc gattctctgc atgtatggga
    10981 tgaagcagca attgtcatgg aaaacctaca tcaagaatac gatctattcc tttcaccaac
    11041 gactgcattt accgccccta aaatcagcga agatttgcaa agtgatgcga taagagcaca
    11101 gatggaacac atcagtgatt gttctgaagt agaaggttta gacgttgttt cttccatgtt
    11161 tgaggagagc ttgaagatca caccttatac acagttggct aatctgactg gacaaccagc
    11221 aatcagcttg cctactcatt tgacaaaaga aaacatgcct ctaggaatcc aatttatggc
    11281 tgccagagga agagaagatt tgctatttca aatcggctat ctatttgaag caaatcatca
    11341 gttccattta ccaaatccat ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa
    11401 agattgtttg ctctaataac tagtgccttt tttattttaa gcgcttgcgg tacagctgcc
    11461 gctaatcagg aagataacag ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg
    11521 acttctggca cactgtttcc cgcatcttat tataatgatc aaaatgaatt agttggttat
    11581 gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa
    11641 tacaacgtgg atggacaaat cacttctgtt tcaaaaagag aatcagattt tgctgcgaac
    11701 gattttggta tcacaaaaga aagagaaaaa aaattctccc tttcagagac tttcaaatat
    11761 tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag gaatccactc acttgaagat
    11821 cttaaaggga aaaaagcagc tggcgaaccc aatactagct atatgcgttt agcggaaaaa
    11881 cacggagcta aattagtcac atatgacaat gccacgaatg atcagtattt aagcgatgta
    11941 gcaaatggtc gaacagatgt tattctaaat gattattatc tgcagaaaat ggccgtatct
    12001 gctttaccag atatccctgt aaaaatactg gagaacgttt atttcaatga aaacgaaagt
    12061 ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa
    12121 gagatgaaag aagatgggac gacaaagaaa atatctgaaa aatattttca aactgatgtt
    12181 tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg agtgatgtac tttcccctaa
    12241 ttgatttttc cctcatgtgg acttcacttc ctttcgtact gcaaggcttg gtatacacat
    12301 tagggattgg cttcgtgtcg tttcttttag gaaatttggt agggttgctt ttgacagttt
    12361 taggtctttt ggattggctg cctctgaatg tatttattcg tttttattta tcgttcttta
    12421 gaggcattcc cgcactagtc ctgctgtttc tgttatattt cgggttacct tatcaattaa
    12481 gtgccttaac ggcttcagtc atctgcttta cgattaccag cagtgcattt attggtgaaa
    12541 tctatcgagg atcactagca ggcgtttctt caggtcagtg ggacgctggt ttagcttctg
    12601 gtttttcttt ctttggggta ttgaaatata tcgttttgcc gcaagctttt cgaatctcag
    12661 taccggcact cagtaacgta gctatggatc tagtgaaagg tacttcgtta gcagcaacaa
    12721 tcacagtgcc agaaatattt caaaaagcaa agatcatagg cggtcggaca ttcgattatt
    12781 tttctctcta tattttagtt gcactgattt attgggtcat ttgtatcggg atcgaaaaag
    12841 tgcaacagta tttagaaaaa cgttctatcc atcagtcaat ctgaaaaata gaaatggtgc
    12901 tagcgtggct ggcaccattt ctattttttg tttcttgtat attctgccta gtattttaaa
    12961 accatttcat gtaaaatgta aatcgaaaat atgttcggag gtagaaaaca tgaagacccc
    13021 gctaaccatc gaaatagaat cctcgttata ccaatattgc atcgagcaag gcggcttggt
    13081 tgtagaagaa gtcacgatgc ctgatgacca aggaattgta gatacgctta gctgcttgac
    13141 aaaatttgat ggaagccgag aatggcgatg ctatgagctg aaagtgacaa aagcagattt
    13201 tcgctctaca gccaaactgt catttgttgg acattataat tattttgttt taccaagtaa
    13261 actatatgaa gaagtaaaaa ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc
    13321 gtacacggtt gaagaggaga tgcctgcctc tggtacattt ctgattgcga aaaaagcagc
    13381 aaagaaagag cttgctgtac cggaagaagc tttgacaaac cgcttcatgg cttctttatt
    13441 cagggaagta agaaaagcaa agcaaatgga gcgaggcgta aaatattttc caaaagatca
    13501 attgtacaaa gaattgaaaa gacgttcaga aaatcaagat ccattttcta acgaacgata
    13561 ttatgaacgg tttttagagg aaatagctag tcaggcaatc aatgagttgc aagaagaagt
    13621 tatcgcgctt cagcatgatt atgaatattt gaggcaacaa cagcagatcc gtcggttgcc
    13681 tacagagcca ttggagtgaa aaatatgtac tatccttatt taagaggaaa acaatttgac
    13741 ctattagcat taaaagaagc actgtcgcga ggattgctct ccaataaaat ccaaccagtg
    13801 atcgaaccag tacgtgattc cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag
    13861 caccatccaa tcgctgtgat ccaaaatccc caagtaggac aattcaaatt attcgatcag
    13921 catgtgcaca gttggacagt caaagaaaat agctctgtcg tccctgcaca gatcttaacg
    13981 ccagaaaatc tcagtgaagt actggattca cctccagcgt ttcttgtttt cgatggacag
    14041 cactacccaa aagatacaga ggtgtggaaa cagttggcag gagttgattc gaaatttttg
    14101 ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac
    14161 tcttttcaaa cgagaaagca tgttgaaagt tatgcagata aaacagatga cttttttagt
    14221 gatgactatc tttttttcca cgcagacgga tatattggat tttctgattt cacgatcgaa
    14281 ggtagccgtt atttcgataa agggttccct agccgagcgc ttgctatcca tctaacgtat
    14341 attgatgctt acggcaatat acgcgtgaaa cattttgtct ctgacagtaa cgactctgct
    14401 aaagaccaag cattgaagtt tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt
    14461 caccatcatc aactcttgat tacttcagga atgattgaat tattggctct ttaccagcaa
    14521 caaaaattcc ctggactggg agtgttgaaa aagtggtcac ttatgcatca tttggaactt
    14581 attagtcacc tgctagatca tccaaaagat tggcttaaat attattcaaa agttcaggaa
    14641 ctatatgaag tgatccaatg attagataat gtaaatgaaa aaggaataga aaagaggaaa
    14701 ctggatgtat attcggcaaa caaggaaaga agaagtagaa gaagtggtag aaatcattaa
    14761 tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc
    14821 aacaaaagaa acgattttga acgatattga aagaaaagaa agctatgtat taatagtgga
    14881 agatcaaatt gctggaacag ctgcattagt ctcaggcgtg gatcccgttt acacaaaaat
    14941 agatggtagt tggaaagaat caactggtcc ttatttatcg attcatcgtg tcgctttagc
    15001 agaaagtact cgaggaaaaa aactagcaaa accgttctta gaacatttgt tgacgattgc
    15061 agccagcaaa aatatccaag atatccgtat cgatacttat ccagctaatg aaccgatgga
    15121 aaagaccatc tattctgctg gattcactta tcaagggatg attgaatttc cttttgaaca
    15181 cggcgaaaga aaagcatatc aaataatcat caattgacta taaaatcatc tgagcaaaga
    15241 taaagacatt aacactactg aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa
    15301 gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa
    15361 ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac
    15421 atagcttttg tcacgatctc tcggcttatc ctacgagtat tattcgatac atttatatca
    15481 ataatccttt ttttatttta tctgcagctt cttggaacgc tccatcgttt gacacgacta
    15541 aaagtgaggg gatagctttc aaacgtttca actgtgctaa atcttctaca taaaccgcca
    15601 gtctggctga aatctgttca gatgaatctc cacgtttttt caggcgcgat tgcaatgttt
    15661 ctttagagat cgtaataaag ataggacgga ctaccgtccc gaaatactca tataaatgcc
    15721 aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac tgtcagtttc tttttaattt
    15781 ctgctttact ggaaccatat ttatgttgag catattggtc ccactctgca aactcatttc
    15841 ccttgatcat ctgattgaat gtattgtcag aaacaaaata ataatcctct tgatcttttt
    15901 ccccaggtct tggtgatcga ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag
    15961 cttctgcgag tgaagttttt ccagaaccag aaggaccgat aaatacatag caataattca
    16021 ttgtcatttc tcctttgatg attgaacaag tgaaaaagct ttggaaaata tttccaaagc
    16081 ttttaaaagt aactttgctt ttttgtattt tataccagca agccagtcac aatagcactt
    16141 aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc cgtgttttgc gacaagattt
    16201 cctttttctt catttaatcc cttcatcgca ccagaaatga tcccgataga agagaagttg
    16261 gcaaatgaaa cgaggaagac agatataatt gctgttgtac gctcagtaaa atggaaatta
    16321 ccatttgcta attctgtcat agcaacaaat tcatttgtaa ccagtttagt cgccatgata
    16381 ctaccggcat ccactgcttc tttccatggg attccgctga tgaaggcaag aggggcaaag
    16441 atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc cgtggaagat agcattgatc
    16501 atggcaatca aagcaacaaa accgataagc atagcagcaa ccgtgatcgc cacatggaaa
    16561 ccatcaagaa tgtattctcc caacatttgg aaaaatgtct gttctttgtt ttcttcaatg
    16621 atcagctcat cttctttttc ttctaataca tagggattta caattgaagc cagaatataa
    16681 ccgccaaata agttcaaaac aagggcagtg atcacatatt tagaatcgat cagtgccata
    16741 taagatccga cgatcgacat agaaactgta gacatagccg atacactcat ggtgaacaaa
    16801 cgtttctcag ataggaaggg taattccttt tttatggaaa taaaaacttc c
//
LOCUS       DAPCMU010000053        16819 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPCMU010000053 DAPCMU010000000
VERSION     DAPCMU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.055"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..455)
                     /locus_tag="RD744_001414"
     CDS             complement(<1..455)
                     /locus_tag="RD744_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDU0557418.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="RD744_001415"
     CDS             complement(560..1423)
                     /locus_tag="RD744_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDU0557419.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="RD744_001416"
     CDS             complement(1619..3718)
                     /locus_tag="RD744_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDU0557420.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="RD744_001417"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="RD744_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDU0557421.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="RD744_001418"
     CDS             complement(5071..6912)
                     /locus_tag="RD744_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDU0557422.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9409)
                     /locus_tag="RD744_001419"
     CDS             complement(7094..9409)
                     /locus_tag="RD744_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0557423.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="RD744_001420"
     CDS             complement(9594..10454)
                     /locus_tag="RD744_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDU0557424.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="RD744_001421"
     CDS             complement(10513..11034)
                     /locus_tag="RD744_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDU0557425.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11312..12073
                     /locus_tag="RD744_001422"
     CDS             11312..12073
                     /locus_tag="RD744_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0557426.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="RD744_001423"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="RD744_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDU0557427.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="RD744_001424"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="RD744_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDU0557428.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="RD744_001425"
     CDS             16433..16789
                     /locus_tag="RD744_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0557429.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5766 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaa
//
LOCUS       DAPCMU010000054        16657 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPCMU010000054 DAPCMU010000000
VERSION     DAPCMU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.056"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..766
                     /locus_tag="RD744_001426"
     CDS             <1..766
                     /locus_tag="RD744_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0557430.1"
                     /translation="TANTVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQ
                     VYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVII
                     ENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNE
                     YVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEW
                     LGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1340..1909
                     /locus_tag="RD744_001427"
     CDS             1340..1909
                     /locus_tag="RD744_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDU0557431.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1896..2756
                     /locus_tag="RD744_001428"
     CDS             1896..2756
                     /locus_tag="RD744_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0557432.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2946..3650
                     /locus_tag="RD744_001429"
     CDS             2946..3650
                     /locus_tag="RD744_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557433.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3655..5490
                     /gene="walK"
                     /locus_tag="RD744_001430"
     CDS             3655..5490
                     /gene="walK"
                     /locus_tag="RD744_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDU0557434.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5487..6803
                     /locus_tag="RD744_001431"
     CDS             5487..6803
                     /locus_tag="RD744_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557435.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6804..7673
                     /locus_tag="RD744_001432"
     CDS             6804..7673
                     /locus_tag="RD744_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDU0557436.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7728..8537
                     /locus_tag="RD744_001433"
     CDS             7728..8537
                     /locus_tag="RD744_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDU0557437.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8640..9269)
                     /locus_tag="RD744_001434"
     CDS             complement(8640..9269)
                     /locus_tag="RD744_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDU0557438.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9439..10731
                     /locus_tag="RD744_001435"
     CDS             9439..10731
                     /locus_tag="RD744_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557439.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10807..11469)
                     /locus_tag="RD744_001436"
     CDS             complement(10807..11469)
                     /locus_tag="RD744_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0557440.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11694..11978
                     /gene="groES"
                     /locus_tag="RD744_001437"
     CDS             11694..11978
                     /gene="groES"
                     /locus_tag="RD744_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDU0557441.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12029..13654
                     /gene="groL"
                     /locus_tag="RD744_001438"
     CDS             12029..13654
                     /gene="groL"
                     /locus_tag="RD744_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDU0557442.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13743..14891)
                     /locus_tag="RD744_001439"
     CDS             complement(13743..14891)
                     /locus_tag="RD744_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0557443.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14993..15577)
                     /locus_tag="RD744_001440"
     CDS             complement(14993..15577)
                     /locus_tag="RD744_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDU0557444.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15704..16135)
                     /locus_tag="RD744_001441"
     CDS             complement(15704..16135)
                     /locus_tag="RD744_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDU0557445.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16153..16473)
                     /locus_tag="RD744_001442"
     CDS             complement(16153..16473)
                     /locus_tag="RD744_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0557446.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5466 a         2845 c         3376 g         4970 t
ORIGIN      
        1 tacagcaaat acggttattc attatcacgc atttatacgc aaagctcttc aagaggctgt
       61 tattacagaa ttgataagta caaatgtagc agataaagta agaaagccaa aaaagaaaca
      121 atttgtttct caagtttata atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga
      181 agaaaaactc tatttggtag ttcttttaac agcattttat ggattgcgaa gaagtgaagt
      241 tttaggatta aaatggtctg ctattgattt tttaaacaaa aaaatcatga taaaccatgt
      301 aatcatagaa aatccagaga atatgtcaca gttaataaag aaagatcaaa caaagaactc
      361 ttccagctat cgtacattac ctttggttga aacgattgaa cgagcattag taaatcaagc
      421 taaatggcaa caagataatc gtattctttt aggagatgat tatattttag aagataatga
      481 gtacgtattt actatggaag atggacggtt gatgaagcct caatatgtaa cgcatcgttt
      541 gagtaagttg attaagaaaa atggattgaa aaaaattcgc tttcatgact tgcgtcattc
      601 taatgcttca ataatgttag atagtggcca gaatatgaaa tccattcaag aatggctggg
      661 acatgcatcg tattcgacaa ctgctaattt atacacgcat ttaaccgcag gagtaaaaga
      721 tcgtgcagca gaagcattag aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca
      781 atatctttgg ggtatcgctt cttgttttta gaattttttt gttggcaatt tgttggcagt
      841 atgttggcaa tgaaaaaatt cacaaactaa aaacctattt ataaagcttt ataaaacttt
      901 tttcacaaat agagcatttg gttaagagtc agctgctcta ccaactgagc taacggcccg
      961 tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa
     1021 gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc
     1081 tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact
     1141 tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata
     1201 aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc
     1261 acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat
     1321 gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga
     1381 actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga
     1441 ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc
     1501 attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt
     1561 taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca
     1621 agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat
     1681 tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca
     1741 attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat
     1801 tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt
     1861 tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt
     1921 tagtagattc aggtacggat gttccagaag aattgataaa aaaatacgag atgtatatga
     1981 tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac attacccctg
     2041 aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt
     2101 cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa
     2161 caatttccag tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag
     2221 aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg
     2281 cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc
     2341 tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc
     2401 aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc
     2461 caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta
     2521 aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct
     2581 tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac
     2641 ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag
     2701 tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca
     2761 aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa
     2821 aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat
     2881 gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc
     2941 tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat
     3001 tcaatttagc caaagaaggc tatgacgttt atactgcata tgatggggaa gaagccttgg
     3061 aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg
     3121 acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa
     3181 cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg
     3241 tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg
     3301 gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt
     3361 tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac
     3421 atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag
     3481 aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg
     3541 taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag
     3601 tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa
     3661 agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt
     3721 ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg
     3781 atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc
     3841 ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga
     3901 tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg
     3961 atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat
     4021 gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg
     4081 gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt
     4141 tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc
     4201 tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt
     4261 tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac
     4321 tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc
     4381 aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt
     4441 ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa
     4501 gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc
     4561 ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta
     4621 gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg
     4681 atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta
     4741 gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc
     4801 tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca
     4861 ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta
     4921 gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat
     4981 acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt
     5041 cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa
     5101 tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat
     5161 aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta
     5221 ttagaaacgc ataataacgt gattttaagt atcaccgatc aaggattggg gattcctaaa
     5281 aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca
     5341 caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc
     5401 gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat
     5461 gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac
     5521 tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag
     5581 gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc
     5641 gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc
     5701 aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat
     5761 ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc
     5821 tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag
     5881 catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta
     5941 ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc
     6001 tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa
     6061 aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta
     6121 cgaaacattc gatcaaactg aagaagtata gttatatcct gtcacagcaa ccctatacac
     6181 tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg
     6241 aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg
     6301 attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc
     6361 cctatattag caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca
     6421 atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag
     6481 gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa
     6541 ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta
     6601 cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag
     6661 tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac
     6721 ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat
     6781 tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg
     6841 cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa
     6901 acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata
     6961 tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc
     7021 agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg
     7081 caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg
     7141 aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca
     7201 aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag
     7261 aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata
     7321 cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca
     7381 tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag
     7441 aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat
     7501 ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg
     7561 tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagc gtcaacgctt
     7621 tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg
     7681 agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa
     7741 aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg
     7801 aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct
     7861 tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac
     7921 atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg
     7981 ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa
     8041 aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg
     8101 gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat
     8161 tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg
     8221 ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc
     8281 cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg
     8341 cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta
     8401 gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa
     8461 aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg
     8521 agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca
     8581 atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat
     8641 tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact
     8701 aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa
     8761 taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct
     8821 ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac
     8881 atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc
     8941 gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg
     9001 gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct
     9061 ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac
     9121 tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg
     9181 attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg
     9241 ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg
     9301 taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc
     9361 tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga
     9421 agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca
     9481 tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca
     9541 ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct
     9601 cagtggatag acaaaacagg attttctatg tattttacac aagtatacca ggctacattt
     9661 gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac
     9721 atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca
     9781 ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca
     9841 actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag
     9901 caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt
     9961 gttatagtca cttttacgct tatcggttca gcggtttttc aacatttgtt cagaatgact
    10021 gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg
    10081 tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga
    10141 tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcaaatgca
    10201 gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag
    10261 caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt
    10321 tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta
    10381 ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg
    10441 atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct
    10501 ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg
    10561 atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg
    10621 tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa
    10681 aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa
    10741 atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag
    10801 tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat
    10861 tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa
    10921 gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag
    10981 caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat
    11041 actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa
    11101 agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt
    11161 gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat
    11221 tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat
    11281 cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata
    11341 catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag
    11401 gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt
    11461 taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga
    11521 ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt
    11581 gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact
    11641 cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa
    11701 aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag
    11761 gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg
    11821 gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc
    11881 aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga
    11941 tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat
    12001 caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc
    12061 acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg
    12121 acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga
    12181 tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa
    12241 acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc
    12301 aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa
    12361 tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca
    12421 taatatctca actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc
    12481 gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg
    12541 tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat
    12601 gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc
    12661 tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat
    12721 tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga
    12781 tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt
    12841 agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc
    12901 tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga aagatgcaac
    12961 aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt
    13021 agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat
    13081 tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc
    13141 aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa
    13201 attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc
    13261 aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg
    13321 agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat
    13381 cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact
    13441 aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt
    13501 agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt
    13561 attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc
    13621 aatggatcca tcaatgatgg gcggtatgat gtagtaatta agaaagaacc ctattttaac
    13681 agggttcttt cttctttttt tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa
    13741 tatcaaaggc tatttagttt atcaattact tgttgtttgg ctctttttgt tacatggtta
    13801 taaatagaca gagttgtgtt tgcatcagaa tgccctacac gctccataat ggctttaaga
    13861 ggtacgccta attcagataa tagtgaaacg tggctatgtc tgaatatatg tgaggataga
    13921 cttttttcta attccaactc ttcctctact ttatggagta tagcgttaaa tgagtgcagc
    13981 gcaagcggag tgccacttgt agatataaat atatattgat cgggatctgt gggtctacct
    14041 gcaagaatat tgtcagctat cacactttca atcaattctt ttgcacgatt gggtaattgc
    14101 acttcacgtt gcgaataagt attttttgga gttgttttta tagcattatc cattttcaca
    14161 gatgtgtaat ctaaggtccc attaatggaa attttcccat cctcatagtc cttcatttgc
    14221 aaagctagca attccccata tctcaaacca gttaaatata gaaactcagc tattatgccg
    14281 tgtagttttc tgcgaggatt ggagtatagc tgtttcagta tttgatcaat ttcttctttc
    14341 tccagatatt ttttattcat agaaagcctt cttttttctt cttccacttt tttaggatgg
    14401 attttaactg ctagcgcagg gtttctttga atgtattttc tatcgattgc atagtttagc
    14461 ataacagata gagttgtttt tgtttgtttt gtgtagttca gtgagaggtc accaaacgta
    14521 tacatatctt caattatctt attaatgagt gtctcatcaa tgtttctaac gatcgtatca
    14581 tcgcttatgt gcttagaaac atgtttcatc atcattggaa ccttcaaata gctagtacgt
    14641 ttaacatgct gcttataata ttcataccat tctttataca gctcaccaaa agtgatatct
    14701 gatttattgt aatcttcgag tgcttcttta atttttttat ctagaatttt ctgagctttt
    14761 ttccacgctt gtggtgaatt acttgtaagt gttgtagatt tttttcgtgt tttttctgta
    14821 taaggatcta catatctttc aataaacttg aattggccat ctttggtctg ttcaacccac
    14881 acttttaaca tctcctatca tttgctataa taggcataac aaatagacct atataggttt
    14941 gttttctaaa agcacgctct tactctggac ggtggggcgt gttttttatt tattatacag
    15001 ttgctttaaa aatagctgct ttttcaaatg atactaaagg agagaagtgg atttctattt
    15061 ctcccttttg gtttaacgtg aaatgagcag ttacatccat ttgttttcct ggagcaacag
    15121 atcccattgt attttcgttg gcatatgtct ctgatttttt gtcatctggt ccatatactt
    15181 ccacatctgt acctacaggg atatctgaat cgccatcatt ttttacggta taagtaattt
    15241 ttactacttg tgcaggttgg ttttcttcaa attgatttct ttcatcagtt agttctacac
    15301 tatttagtgt atattcagca tcaccaactt ttacagtgtc accaatctta tagaaggtat
    15361 cgcttttttc ttctttagaa gatgaagcag aggttgaaga tttagttact ttttcgccac
    15421 cattatcact tgctttatta ctatctgatc caccatttaa agcagaacca ataattataa
    15481 ttaaaattac tgctaatacc caaaaccata cttttttgta aaaaggttta ctaactttat
    15541 acatttttcc gtcttgaccc ataacttttt ttgccattta aatattcctc acttcttgtt
    15601 ataatatatt tgcgatctca gaaatgaggt atgagtccgt gtgccagcac gggctttttt
    15661 aatgttttgc tgaaatcggt ttttttaata attcttgatg tattcataca tattgccttg
    15721 cgtaagaata ttttttctta aaatagcatt ggcagacagc ataacaagtg tatctgagct
    15781 tattctgaat agaataatac tccataaatt tttcgagatt aaactgagat tcatcagtca
    15841 gttcgttctc aatatagata tttaataaaa ttagaatagc tattttatct gcttctgttt
    15901 caaattttga gtgaaaagtt gtagaagtat cgtacaaaac tgaatattca aaatgagaag
    15961 cggtgaaatg cgcaagttca tgagataaat gaaaggcttc tgcagtttcg ccatatagat
    16021 tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct ttcatttcca
    16081 tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa tacatacaac
    16141 ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg ctttacgcat
    16201 tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc
    16261 cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag agactccaaa
    16321 gtaatcagct aaccgagcta actcaataga attaggagtt tgtcttttcc aacttcctaa
    16381 atatccattc gaatacccga aattcatttc gagctgtcta atggataatc ctttttcttt
    16441 tgctaactcc tttattattt cgtaagtatt cattgataaa tcaacctttc tgaatgctta
    16501 caaaaaaagt ttagataaat aatgtaaaaa tagttgactt atatacatta ttaatctata
    16561 ctatgttttg taaacaagtt taacaactaa aaagacaaca aaaaatacta ttgattaata
    16621 aatgccaacc gccaagaaag ctttttaaat caaagtt
//
LOCUS       DAPCMU010000055        16551 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPCMU010000055 DAPCMU010000000
VERSION     DAPCMU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.057"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(61..2766)
                     /locus_tag="RD744_001443"
     CDS             complement(61..2766)
                     /locus_tag="RD744_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDU0557447.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3072..4019)
                     /locus_tag="RD744_001444"
     CDS             complement(3072..4019)
                     /locus_tag="RD744_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDU0557448.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4209..4478
                     /gene="rpsN"
                     /locus_tag="RD744_001445"
     CDS             4209..4478
                     /gene="rpsN"
                     /locus_tag="RD744_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDU0557449.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4654..5016)
                     /locus_tag="RD744_001446"
     CDS             complement(4654..5016)
                     /locus_tag="RD744_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDU0557450.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5027..6148)
                     /locus_tag="RD744_001447"
     CDS             complement(5027..6148)
                     /locus_tag="RD744_001447"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDU0557451.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6164..6514)
                     /locus_tag="RD744_001448"
     CDS             complement(6164..6514)
                     /locus_tag="RD744_001448"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDU0557452.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6644..8155)
                     /locus_tag="RD744_001449"
     CDS             complement(6644..8155)
                     /locus_tag="RD744_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDU0557453.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8477..9829)
                     /locus_tag="RD744_001450"
     CDS             complement(8477..9829)
                     /locus_tag="RD744_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDU0557454.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(9982..11058)
                     /locus_tag="RD744_001451"
     CDS             complement(9982..11058)
                     /locus_tag="RD744_001451"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDU0557455.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11254..12999
                     /locus_tag="RD744_001452"
     CDS             11254..12999
                     /locus_tag="RD744_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDU0557456.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13279..13716)
                     /locus_tag="RD744_001453"
     CDS             complement(13279..13716)
                     /locus_tag="RD744_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDU0557457.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(13700..15073)
                     /locus_tag="RD744_001454"
     CDS             complement(13700..15073)
                     /locus_tag="RD744_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557458.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15395..15883)
                     /locus_tag="RD744_001455"
     CDS             complement(15395..15883)
                     /locus_tag="RD744_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557459.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(15905..16507)
                     /locus_tag="RD744_001456"
     CDS             complement(15905..16507)
                     /locus_tag="RD744_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDU0557460.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4641 a         3548 c         3010 g         5352 t
ORIGIN      
        1 tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca acctcttttt
       61 ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt tttcttcgat
      121 gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga tcatgatcca
      181 gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta acaagtttac
      241 cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg agacttggat
      301 cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag cgatcccttt
      361 accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg ctaaggcgat
      421 caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta gtaagaaaat
      481 attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg
      541 agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta aatagtattc
      601 tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg cagtataaaa
      661 cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt gtagctcaac
      721 aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt tgatgacttc
      781 gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt ggataccagc
      841 tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac
      901 ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg
      961 ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga tcggtaattc
     1021 attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca tatttccatt
     1081 cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt tttgatattt
     1141 ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa
     1201 gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg
     1261 gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag caatgatgcc
     1321 attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag ccttcgtgtc
     1381 ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc ttagcaaggg
     1441 ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag cacttgtttg
     1501 caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag atattccgtc
     1561 aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca gcttaccatc
     1621 ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca cttgtccaat
     1681 tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt tcagctgaga
     1741 ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat ttttcacatc
     1801 tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt catcgattcc
     1861 ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc
     1921 ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg cttgatcttt
     1981 caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat ctactttagg
     2041 gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag gtagttgact
     2101 ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga
     2161 taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt cgtaacctat
     2221 ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat ttgtgatttg
     2281 cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat tgattttttc
     2341 atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac tcagcagatc
     2401 ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac ggactccgtc
     2461 ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt tatgaagcga
     2521 ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg gcttgtctgc
     2581 actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata atgctttgat
     2641 gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa gtgttgcaat
     2701 cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat ttttgattga
     2761 ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg tatttcatac
     2821 ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg gcaattgtct
     2881 cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac tttatcatga
     2941 aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta tacgcaaaaa
     3001 agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt cgttttttat
     3061 tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg atgtccttca
     3121 actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat tgaaataaaa
     3181 acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc tgcatctaga
     3241 tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg caaatctaga
     3301 tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg gatgccggtt
     3361 tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc
     3421 agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt ttttaccttt
     3481 tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg gagaagctgg
     3541 atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc tgtcggctgg
     3601 atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac cattccggct
     3661 gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg caacacgact
     3721 tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac atgaacagtt
     3781 gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg tacataaggg
     3841 aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc ataaggattc
     3901 acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct gggatgagca
     3961 tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg
     4021 gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac aaaaaaaatg
     4081 tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt
     4141 aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat aattggagga
     4201 atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg
     4261 aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa ggcttgcgta
     4321 aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc
     4381 ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa ttagcacata
     4441 aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa
     4501 aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc gcttcttcta
     4561 gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg gtcctttttt
     4621 atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag tcagtgcttc
     4681 gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc gatccaaatg
     4741 gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca acaggataac
     4801 ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg gaagcttagg
     4861 tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat ggttacctag
     4921 attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca cgactggaga
     4981 aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat tcttgatatt
     5041 ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta cctaattgat
     5101 ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc gtcacttttg
     5161 ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat acacgcccaa
     5221 tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt cgtggccagc
     5281 catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg atatatgttt
     5341 ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat tcagaaacca
     5401 gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag ggattaagtc
     5461 cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc cataaagccg
     5521 ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc agcacttcta
     5581 taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct gtagaaaaat
     5641 gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca gctgcctctt
     5701 ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct tttagatgaa
     5761 tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct tgcaaccatt
     5821 cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt gcgtcgtaag
     5881 gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac tcgactcctt
     5941 catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc tcagctgttt
     6001 caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct tttccttgcg
     6061 gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt atcactgctt
     6121 tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct tccaaaataa
     6181 tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg ccttcatgag
     6241 gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca tcttgaaagg
     6301 taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc
     6361 cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc atttcatcag
     6421 aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg atcctaggaa
     6481 gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc aataaattta
     6541 gaacactctc attctaccaa attataacca ttttgccaca aataagtcca caaataacaa
     6601 aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg ttatttgaac
     6661 ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt tggctgttat
     6721 tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg tcacgacgat
     6781 tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc tgcattggca
     6841 atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt gctgtcgttt
     6901 ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta tctgctgttt
     6961 gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca actgcagcac
     7021 ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc atccgttttt
     7081 tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag
     7141 acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag ctttctggat
     7201 cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat ccacgtgcag
     7261 caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc aatacgctca
     7321 ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg cgtgcttcta
     7381 atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt gttaattcag
     7441 gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct tttgcgcgga
     7501 catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc acatgatgtg
     7561 gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt gctgagaaaa
     7621 gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct tccaagaagc
     7681 ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt ccaagtttca
     7741 atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg atatgtggac
     7801 gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg gcttgtacac
     7861 gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata gctaattcac
     7921 gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca
     7981 ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca ataacgtctt
     8041 ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct tcaaaaccag
     8101 cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat ttcaaatgaa
     8161 tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc gatttgttcg
     8221 gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca ttcttagccc
     8281 ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc caaacgggtc
     8341 gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct atggcaaaac
     8401 cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt ttcgttttct
     8461 gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca aataagccca
     8521 tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt ttgatagcat
     8581 caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg tatttttggt
     8641 tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg gcgaatttct
     8701 caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc cctaattcca
     8761 gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta aaagtatcta
     8821 ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata gctgcaccat
     8881 ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg agcccttggc
     8941 gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg
     9001 ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact acaaaatcgg
     9061 tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca ataccgaatg
     9121 ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca tctgcgggca
     9181 caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct ttagcgattc
     9241 cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt gctgcagctt
     9301 ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg cccatttcca
     9361 aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat
     9421 tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca gacgcggtta
     9481 gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct gcgcctactt
     9541 tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct acaggaataa
     9601 ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca gatgctccgt
     9661 ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga ggaacgaata
     9721 aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag
     9781 agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt gagatctctc
     9841 ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg actctcactt
     9901 ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac gcaatcactc
     9961 cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac gctgatggta
    10021 tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca agcccatatt
    10081 ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt ttaattcatt
    10141 caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc
    10201 taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt cttctggcac
    10261 ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat aatcatagaa
    10321 tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat ttcctaatac
    10381 agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag aatcatactg
    10441 ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg taatgccgac
    10501 actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac cttcacattg
    10561 atcaaatact tttttaggat tttctttcca ttgattctta agtactggta cataaggaac
    10621 ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca tggcacatgc
    10681 actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga agccttggat
    10741 cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc cttcttcttt
    10801 gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc cacttgggtc
    10861 ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg gacctttgac
    10921 ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat aataaatggc
    10981 atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc gtccgccata
    11041 tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag tgatttactc
    11101 tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa acgagccagc
    11161 ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc cgttttttat
    11221 aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc acgcagccgc
    11281 ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt tgtacagcaa
    11341 aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt catcaagata
    11401 ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca aacattcaaa
    11461 acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca
    11521 gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc ttttgcctct
    11581 gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag
    11641 atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt tgaacgagcg
    11701 atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg ggtactccaa
    11761 gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca aaatggcgaa
    11821 caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat ttatgacgct
    11881 ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta
    11941 atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa
    12001 caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga tgatggtttt
    12061 ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa agaagaacag
    12121 acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc ctttgctttt
    12181 cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta catcgatttt
    12241 ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc cgttttagcg
    12301 ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa
    12361 ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga acagtattat
    12421 gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt gacgatttgt
    12481 catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc atttgggaca
    12541 ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt cgcttcgcct
    12601 tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa acaaagcgga
    12661 ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct ccaccatcta
    12721 ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa
    12781 ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat tttagctgca
    12841 ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt
    12901 tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt tttggcaatc
    12961 gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt gacagaaaga
    13021 atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt tttgtgaaaa
    13081 ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag
    13141 ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca tttatcctgt
    13201 ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt acctactatc
    13261 ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa aagacatatg
    13321 atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc ttaactttcc
    13381 actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga
    13441 gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt
    13501 gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac tatcagctcc
    13561 gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat aatggtccac
    13621 actggtcacg ataaccacgt caagaccata ggattctcct aattttatga catcttcttt
    13681 tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc ttccataaat
    13741 gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt gccaagataa
    13801 gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat aatagatgga
    13861 ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa aactggtgga
    13921 tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc attggaatag
    13981 ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg tgccagaaac
    14041 taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac
    14101 tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa atccatggtc
    14161 cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa atcgaaagtt
    14221 tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg aaattcccta
    14281 gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat tccttcttct
    14341 ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa atagcaagat
    14401 caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat aagccaaaga
    14461 atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg tctttgaaat
    14521 ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa aaaagtgtat
    14581 aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga
    14641 gaaatagcca gatagctgca aataagtact ttttcccatc catattgata aaaccttgcg
    14701 tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca ctcagcccta
    14761 gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt ccaagcaatg
    14821 aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct agaccaaatc
    14881 gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc gtaaatggca
    14941 aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa
    15001 taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca aatcgtttga
    15061 gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc tcttcgattt
    15121 tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa aaaaatacag
    15181 aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag cagacaagcc
    15241 cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa gatagaaatc
    15301 aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc ttctcgaccc
    15361 gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt ttcattttat
    15421 aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta atctcaaatc
    15481 cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc aaaaagatct
    15541 ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa gaagtcaatc
    15601 cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt ccatttatct
    15661 cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag taggacacac
    15721 cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc atgtaatcca
    15781 cttgtttttg accaatgatc ggcacaaaaa cttccggcca tacttcttgt atgactgcca
    15841 tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca cgtccttttt
    15901 tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg ctatacacac
    15961 ccaccgacca cggtttcaag aaaatcacaa tctgcataaa ttccctaaaa gataatttcg
    16021 tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa
    16081 ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact
    16141 tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca agcaaaaatc
    16201 cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat ccattgccaa
    16261 acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg atcacttgga
    16321 tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg ataaacgctt
    16381 gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc aacacaaaga
    16441 aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact gatccttttt
    16501 tagacatcta tattccccac atcttttttc atttattaaa actgcctcct t
//
LOCUS       DAPCMU010000056        16501 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPCMU010000056 DAPCMU010000000
VERSION     DAPCMU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.058"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..546
                     /locus_tag="RD744_001457"
     CDS             <1..546
                     /locus_tag="RD744_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDU0557461.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
     gene            735..1403
                     /locus_tag="RD744_001458"
     CDS             735..1403
                     /locus_tag="RD744_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDU0557462.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1603..1989)
                     /locus_tag="RD744_001459"
     CDS             complement(1603..1989)
                     /locus_tag="RD744_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557463.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1961..3343)
                     /locus_tag="RD744_001460"
     CDS             complement(1961..3343)
                     /locus_tag="RD744_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDU0557464.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3499..4686)
                     /locus_tag="RD744_001461"
     CDS             complement(3499..4686)
                     /locus_tag="RD744_001461"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDU0557465.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4693..5724)
                     /locus_tag="RD744_001462"
     CDS             complement(4693..5724)
                     /locus_tag="RD744_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDU0557466.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5740..7194)
                     /locus_tag="RD744_001463"
     CDS             complement(5740..7194)
                     /locus_tag="RD744_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0557467.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7369..8274
                     /locus_tag="RD744_001464"
     CDS             7369..8274
                     /locus_tag="RD744_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDU0557468.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8365..9042
                     /locus_tag="RD744_001465"
     CDS             8365..9042
                     /locus_tag="RD744_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDU0557469.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9039..9209
                     /locus_tag="RD744_001466"
     CDS             9039..9209
                     /locus_tag="RD744_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557470.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9323..10219
                     /locus_tag="RD744_001467"
     CDS             9323..10219
                     /locus_tag="RD744_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0557471.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10274..11644)
                     /locus_tag="RD744_001468"
     CDS             complement(10274..11644)
                     /locus_tag="RD744_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0557472.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11882..12628
                     /locus_tag="RD744_001469"
     CDS             11882..12628
                     /locus_tag="RD744_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDU0557473.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12625..13464
                     /locus_tag="RD744_001470"
     CDS             12625..13464
                     /locus_tag="RD744_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDU0557474.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13465..14415
                     /locus_tag="RD744_001471"
     CDS             13465..14415
                     /locus_tag="RD744_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDU0557475.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14556..15308
                     /locus_tag="RD744_001472"
     CDS             14556..15308
                     /locus_tag="RD744_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557476.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15327..16478
                     /gene="queG"
                     /locus_tag="RD744_001473"
     CDS             15327..16478
                     /gene="queG"
                     /locus_tag="RD744_001473"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDU0557477.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5369 a         3091 c         3316 g         4725 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtctc gtccaatttc gtaaaacaac cgtaacggtt
      541 gtgtaaaaat atcattttag agcataaaaa aaaggagtgg atttctccac tccaaaaaag
      601 tctaactttt gggggtcact acagtgtcac agtccttttt ttagtaaaag gatacatttc
      661 ttccttgtat attccatgcg tgttcgtgag aaagcatata taatttaatt aacagatagt
      721 tagggggaga aaaaatgatt ttttcaatta caatgaaact ggtgttcggg ttgatcggtt
      781 tattattagt ggtacgtttg cttgggaaga aatccatgtc agaaatcaca ccgtttgatt
      841 tagtctatac gcttgtttta ggtgggatct tagaagaatc aacctatgat gacaatgtcc
      901 atgtagggca tgtcctattt gccattgcgc tttgggcagt aatgatttat ggtatcgaac
      961 gtatcgtcca aaaaaatgaa aaggtcaatc gttgggtcaa aggcgagcca tctgttctaa
     1021 tcaaagacgg cgtcatcaac atgacagagt tgacaaataa ccatatcgag atggagcagt
     1081 tgcgagcaat cttaagacag caagaatgct tttctcttga aaatgccaaa catgttatcc
     1141 tagagaatgc tggtcaaatg agtgtattga aaaaatcaga tgaagacaaa gctttatcga
     1201 tcctgcttgt agatgaaggg caaattcagc ataaagtgct tcagtccaat caattgactg
     1261 aagcatggct tatggagaac ttgaagaaag aaggctatgc agatgtaaaa cagctgattt
     1321 atgttgaatg gtcagaagaa aaagggctct acatactaaa taaagaagac gtcatgcatg
     1381 aatcttaccg aatagatgga tagatagaca gaggttgtga aatcagcgcc ttggcttgtt
     1441 actcaaaact aagcgacttt ttcacaacct ctgatatcta atatacgttg ttccagaagt
     1501 tgcttcttcg aaaaataagc caaaattcca caaaaattta agaagcaatt ttcgaaaaat
     1561 cctttcttat tttttgaagt tgatcacttc tgtcacaacc tctcatgtca caaaccattt
     1621 ttttgtttcc tttaactcaa tataccgtac gtcttcccac aatatcggct gatggtcaat
     1681 cataaagtaa tcttcatagg cttcaccaat gaagtgccct tcgacctcat ccaagagtct
     1741 tccaaattca tcttttaaat tgagctgaat gattactgct ttacgagata aaaaggcttc
     1801 tgtcagtata ctatcgattt cttgcaatgt catttgttgt ttaggaagag gctgtttgcg
     1861 tgcttcttct tcattttttt tgataccgtt gactagttct tccatcgcaa aagcagtccc
     1921 ccattttaat ccaaatggcc gatctcgata ctcgttatag tcaatgaatt tttttgctgt
     1981 ccgtctcatc ttttccacct tctaatccac tcataccacc tgcatgcccg ccaacgagag
     2041 aactacgtgc gatggcgcgt cctccttgca agagactatg cgcatggatg attgattgaa
     2101 agccgtattt ttttctaatc gtatcgacaa caaaatcaac tttcgcttca tttatctgct
     2161 tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc gtatacgagg tcggaacagt
     2221 tcacgcctac acgccgaata tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag
     2281 catctgctag atctctgcta gcatttgtcg cttcgatttt catttgctgg tgaaacccgg
     2341 tcttgccatt tggatcaaga tagcctaaag agtatcctac ccataagctg actacttgtg
     2401 tttttgctgc agatcgtctc aagcgagtgc cgacttggtc ggccatttct ttgataacga
     2461 tctcgatttc ttctcgtctt gtataatctc tatctaggat ttgactattt ccgatacttt
     2521 ttgattttgg tgtatattct tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat
     2581 aaagctgagt accgatgacc cctagcttgc ttttcaagtg ataataatcc gcgtgagcta
     2641 aatcataaac agaatgtata cccatttgtt tcagacgttt tgctgttcgt ttgccgattc
     2701 cccaaaagtc agtgatatct gagattttcc ataacttttc tggtacatct tcataacgcc
     2761 attcagcaaa catttgcttt ccgtcttttg agccattgtc taaggctagt ttagcaagta
     2821 gcgggttatc gccaattcct atagtcgtgt agattccggt ctgctcaaac acatctttat
     2881 ggatcagttt agccatgtcg tacgcatttg ttttaccaaa gagtgccatg ctatttgtta
     2941 catcaaggaa gctttcatcg acggaataga catgatggtt cgcttcgtct gcatattttt
     3001 tatagataga attgatttct ctgttctttt ccatataata ggccatcctt ggggcaacaa
     3061 tatataaatc acttggatag gggaagggaa gatctctggc acggctgata ttggtgatgc
     3121 cgtaagcttt ttttgctgca ggactggaag ccaagattaa tccgctaccc cgtgtttctg
     3181 tcgaatcgga cggataggac atgactacta atttagttgt aaggggatca agatcctttt
     3241 caacgcactc tacggaggcg taaaaagatt tgcagtcaat acataaaata tctcttgaag
     3301 gttctttttc ataaacaaat accggatttt gattcgccca cataacaatc acctcttcaa
     3361 cacaattaga acacacgttc tctttccgtg tcaataaaaa acagcaattt tcaaatacga
     3421 gcaaaaggct cacatttgga aattgctgag aggttatttt tatctatttg tttttagctg
     3481 agatcgttga tttttttatc aaacatgaac agcttctgga tcgatctttg ttgtcccatt
     3541 tccaatgtgt gcaacataaa attccggagc atagccgaca actttcatgt attcatcata
     3601 gacattgttt ttgaaggcag gtatattttc ttctttgaca agagcaatcg cacagccacc
     3661 aaatccagct cctgtcattc gtgcacctaa gacaccttct tgtttttgag cagcatctac
     3721 tagtgtatct aattcgatcc cagttacttc ataatcatag cgaagagagt ggtgagaatc
     3781 atttaacagt tgaccaaatg cttctaagtt cccagcttct aattcagctt ttgccttcaa
     3841 agtccgctga ttttcagaaa cggcatggcg agctctgcgt atcaatgtat catcgccgat
     3901 ttggtcaata gttgattcaa attcttcttc atctaagtct cctaaagaag agataggcaa
     3961 agttttttgc aaacgtgcca aggcttcttc gcattcgcta cggcgttcat tgtattttga
     4021 atctgccaat tcccgccgct tattcgtatt catgatgacg attgcataac catctagttt
     4081 cactggaacc atttcgtatt tcagcgtatt tgtatctaat aagattgcat gatctagctc
     4141 accgaagccg atggcaaact gatccatgat acctgaattg acaccgatga attcattttc
     4201 tactttttta cctgtttgga ctagttccaa gcgatctgtt tttaaatcaa acagatcttc
     4261 taatacaaca cctactaata attctaatga agcgctactg gaaagtcctg caccattagg
     4321 gattgttcct tccactaaca actcaaaccc atgatcgatt gagtgtccag cttcttttaa
     4381 tttaaggacc attcctttta cgtaatttgc ccagtttgct tttttatcat attctagctc
     4441 atctaaagta aaactaatga caccgatatc gtcaaagttt gtggaataaa ctaatatttt
     4501 attatcgtct cgaggtgctg ctacaccttg cgttccataa gtgatggaag cggggaagac
     4561 atggcctcca ttgtaatctg tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta
     4621 ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat
     4681 ttccatgatg agttattcct ttcttgtttt gattttgaat tctgtcactg tttcaaatac
     4741 tgatccagct tttaaatgaa tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt
     4801 ttgagtctca aatgtgatgc caccgtgatg tgccagtttt cggttgcgca tttctggtgt
     4861 ttctgtgccg aaatttgcag tgaaaataac cacactagaa gcatctgtag caatacctac
     4921 ttggattctt ttatcggggc tagtcaagcg agcggcttct ttacccaagc cggtttcttt
     4981 taagaaaaat ggatgatcga ttccgtcaac aagatttttt tgatccaaat cactagcaaa
     5041 aacggacgct aattttttag gtatttgaaa atcgaaagca cttcctgcag cattttcttt
     5101 tacccctata ggaatactat ctgttcgcaa aggagcataa gcttcgctat tgagccaaag
     5161 ctcatgttga tcgattgact gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt
     5221 cggattgaat aaagtatctt ggtcactggt gccgcgagag gtcactcgcc agatattatc
     5281 ttttgtcaaa gtatagcgga tttccacatc cattgtacct ggaaaaccat gttctccgtc
     5341 gggacttgta gtagtaaaaa tgacagaagc ttcattttcg ccattcaaaa tgacataaga
     5401 ccattttttt aattcaaatc caggagcacc accatgcaga ttatgtccag tttttggatc
     5461 tgtggctaat tggtaagttt ttccattgag agaaaagcgg ccattttcga ttctccctgc
     5521 ggtgcgaccg atagaagcac cgatatacgg atctttttcg atatattctt cagcggtgtc
     5581 aaatcccaaa actaattctc gatcatttga ttttaaactg acgattctag cacctaaatc
     5641 agagatagaa agtgctagct tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc
     5701 aaaatctttt tctgtaatct tcattttcaa gatcctttct taatgggaag gaactactgc
     5761 atcgtgaagt tcatcttcca ttgtatcagc tggagccaat ttgttttcca attcctcaac
     5821 gatttgtgca tgttttttct ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa
     5881 aatcatcaaa gcagcaggag catagaatgc ataagctttg aaagtcgtta cgccatgagc
     5941 agtgatatca gcagcagtag cattaccagt catcccagaa gcgatagcga caaaccctac
     6001 gatcccgtta gacaaagcac cagcgatttt atctagtaaa gggcgaattg atagagtaac
     6061 agcttcgtta cggataccat ttttccattg accatattca acagagtctg tgatcgtcat
     6121 caaagcggat aagaaaatca attgttgagg gaagtagaac aatactagcc cgatgataac
     6181 gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat
     6241 ccctccaagg taaacgtagc gacgagagat gacgcgtgta agcattggga atagtggaac
     6301 agctactaaa ccaagtaagg cagcaactag accaacgatc caaaattcag ttggttggcc
     6361 aagtacatat ttgaagaagt agaataatac ggcagttgtt gctacattgg caatcgcata
     6421 agcgatataa gcaagggcaa gccacatcaa ctgatcattt tgagcaactg ctttgaacac
     6481 atctttgatc ccagcttttt cattttgctg acggatcgca gtatcgactt cttttgtgcc
     6541 caaggcagtg ataatcgcag taccgcctga aataacagca gcaatcacac cgaaccagaa
     6601 ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga taggtaaaga aagtaacgat
     6661 agggacaacg actagagtag taccatttgc acctagagaa gagccgaaac gagcaaacgt
     6721 agcaaatttt tcacgttcct ttgtttccgt actcaaagct ggaatcattg accaaaaagc
     6781 gatatcctta aatgaataaa aactatccaa taagatgaaa acgattgcaa ataatatgat
     6841 aaataaagtc gtattatgag tagctaagcc aaagaaattg gtaaagattg ctaccaagag
     6901 tacggaacta acaacgccgc cgataactaa ccatggtttg aatttaccgt attttgtacg
     6961 agtattatcg atgatgccac caatcaaagg atcaaaaata atttcaacta agcggatacc
     7021 tacaactaag cttgtaactg tagcaatcat ttttgcattc gttgccttat ctgttcctgc
     7081 gaacatctgg cttgtcacga ataccataaa ataagtgctt aatgtaacat agaatgtgtc
     7141 gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc attttgcttt tcataatctg
     7201 tattcctctc ctgttcaata tataatacta agtaaaccgg ttacaacgaa taacaaagat
     7261 cttttaatga cttaaactta acaagaaatc gcttcctttt taatatattt ctgtgacttt
     7321 tatttaactt tttgttgcat tatactaaag gagtaaggag ggatagacgt ggagcaatcg
     7381 atttttttaa aaaaggaaca tcaacaagta gaaaaagaat tgtattttga tttttgtggt
     7441 ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta ttcgcaatga ttacatcctg
     7501 catgtcgtga tggaaggcaa aggatcttat catgtgaaaa atcaacaatt ccaattgaaa
     7561 aaaggtgatc tgttcttgat tcgtccagga gactcgacgt tttacttatc agatggagaa
     7621 gatccgtgga tgtattgctg gctttcattt ggtgggagtg ctgcagaaga aatcattcgg
     7681 cagtcgcttt tcagagaaga ccaatacacg atggtctctt ctgacataca aaaatatatc
     7741 gacattattt tagaatgcat gaaagatacg gaagaaacct tggaaaatga attacagtta
     7801 acagcattaa cctaccgttt tttatcagtc cttttaaaag atggcggaaa ggtcagcggt
     7861 ggtgtacaaa aggttcattc ctctttagct gttgaaacag tcaaatacat cgaggcacat
     7921 tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt tagcggtcaa tcggagccat
     7981 ttgtcgcgag tattcaaaaa tcatatgggt accagtatca aggaatattt gatcggggta
     8041 cggatcaatc gaggggcgtt tcttctttct ctgacggatg aatccgtgga aagtattgcc
     8101 tatcaagtag ggttcaatag tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga
     8161 gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa attttaagaa tattgctttg
     8221 gataccttga aaaaacagtt ggaaaaacaa accgcagttt cttggacaac ctgattagtc
     8281 tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg gttatgctac actgtaaagc
     8341 gaagactaaa aaggaggaaa agggatgcgt acacttatca tcattagcca tccaacgatc
     8401 gaagaatctt taaatcagca atttttcaaa gaagcttctg ctggtttacc agctacttgg
     8461 catcatttgg aatcaagtta tccagacgga aaaatagata tagagactga actacagctc
     8521 ttggcccagc atgatcggat cattttccag tttccgtttt actggtatag ttctcctgct
     8581 catttaaaaa tttggcaaga taatgtcttg gaacgagcag cacatgtatt aaaagggaaa
     8641 gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa
     8701 gaagaatata cgatttccga atttttgagg ccgtatcagc gaatcgcaaa taaattccat
     8761 atgacctatc ttccgccatt cgttttagca caatttatgt atttatccca ggaaaaacgt
     8821 tgggagaagc taattgctta tcagcagtat ctctcattgg aaggcaaacc atcgctgact
     8881 cagcggattg actggtttat tcaaagagta caggaaaatc agaaaatgca ggaagaagaa
     8941 tctgaaaaac aagcatatat cgtagaagca ctgactgagg caaaagagca gatcgaagac
     9001 ttgaccttta cactgcaaga aatgaaagga acttcattat gacaaatgat tggctgatcc
     9061 aacaattaga tgaattaagc gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa
     9121 taaagaaact gatggctgag atagacgaac gaacaatgca gctgcaagga gaaatcgacg
     9181 gacaatcttg gaatcatctc aactggtgaa attttcttat aaataacaaa gataaaaatg
     9241 tgcacagcga caattatttg ctacaataag aatatgaagc gtttgataac gctattatac
     9301 ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag tggggagaaa atgttcggat
     9361 gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg atgatcatga ttggggcagc
     9421 agttgccgtg tttttgatca tagcaggagg agttgcgggt ctatattttc atggaaaaaa
     9481 ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg acaaagaaaa cagagacgaa
     9541 acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa acttctgaag aaaaaacagc
     9601 aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga
     9661 aaagacgcct gcattgattg aagaatacca gacagaaatt cagggaaatc aagagggaat
     9721 agctggttta tcagcagttg ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga
     9781 agggatcaga aaattacgtg cagcgtatca ggcagcagaa aataaagacg gtgtagactt
     9841 agatactttg atcaatcaat tatcggctaa ttatacgaat catgtcgcta agatttccga
     9901 tatttatttg cagactagcg cctctcttca agcagagtca acaagcacac aagacacaac
     9961 atcttctgat tctgagacag cagagtcggt agaagcttct gaaatgcaag ctcgaacaac
    10021 acaagacaca acagattcct ctgaaactaa taccaacagc gcgacttcac aggctgcgac
    10081 aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt
    10141 agaaacgatc ttgtcgctga atgggatgag catggagaat tatttcctga ctcccggtga
    10201 gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg aagcttccac taaggtgaag
    10261 cttcgttctt tttttatttt ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt
    10321 aaagcaagaa aacgagtcca ataatcaatg agattctcgt ttcaggattg ataaacatga
    10381 aaatcaatac gatagctaaa aaagcaagtg tcacataatt cgtataagga gatagtggca
    10441 ttttaaaagg atgatcatgc attttatctg cgtgcttctt tttgaagcga atctcgctga
    10501 tcaaaatcac tacccaagga accataccag gcaaaatact tgagctgtat acgaaaacga
    10561 aaatattttc tgactggttg atgaacattg gtagaagata atttaagatc agaccaatta
    10621 agattccacc tgagattgct acgacaggat aaaaaggcgc accgtgacga gatagtttca
    10681 gaaatttttt agacatcttt ccatttatgg caagtgtata gaccatacga cttgcgctga
    10741 aaatccctga attgcaacct gataatgccg ctgtaatcac gacaaaatta atcagtcctg
    10801 cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa aggtgaacca atctcactca
    10861 attgattcca cggatagata ctcacaatga taaaaatagc accaatataa aaaatcaaga
    10921 ttcgaccgat agtggactgg acagcttcaa tgatcgtctt tttaggattc tctgcttcac
    10981 cagcagtcat tccgatcagt tcgattcctt gataggaagc aacaacgatc gataatgcaa
    11041 agaaaaatcc tttgacgcct ccagtgaaga atccaccatt tttccaaaga tttgaaatcc
    11101 ctactgcctg accatgatta ccaaatccaa ataagattac cccaagacct gcaatgatca
    11161 tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct
    11221 tgacagaaat aagatttgcc agcataagaa acaagacgac aatgattccg ggaataattg
    11281 ccggcaaatc aggccaccaa taattcatat aagatcctac agcaatcact tcacttattc
    11341 ctacgacaat atattgaaaa atattactcc aagcggtcaa gtagccagcc acaggatgga
    11401 tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc
    11461 ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat cgcataggct cccatcactg
    11521 agggccctgt ccaacgtatg gttgaggagg cacccataaa cagaccgaca ccgatcgttc
    11581 cacctagtgc gatcatttgg atatgacggg cactaagact tcgagttaac ttttcttctt
    11641 ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca
    11701 catgtttttc attattaggt tagccttatt gaactcttga tgtgattcta cagaaaattt
    11761 tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt
    11821 ccattttagt taaactatat ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga
    11881 tttggaacgt acggccattg taattcaaga cctttcagcc gcttatcaag ggaaaactgt
    11941 tttgaaaact atcaacttga cgataaatcc acaaaaaatt accggtatta tcggaccaaa
    12001 cggtgcagga aaatcaacat ttatgaaatc tttgctagag ttgataccag ctactactgg
    12061 taaagtcacc ttttctggac agccggtaaa ctcagtccgt aaaaaaatag cctacgtgga
    12121 acagcgaagt gaattggatc tttcctttcc aattgatgta ttaggcgttg tacttttagg
    12181 aacatatcca tctttacgaa ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag
    12241 acaagctttg aaaaaagtag ggatggaaga atatgccaaa agacagatca gcgaactatc
    12301 gggtggacag cttcagagag tttttattgc aagagctcta gcccaaggag cagaatggat
    12361 ctttttagat gaaccattcg tagggattga tgcgttaagt gaacgaaaga tctttgacat
    12421 cttgcaggaa ttgaagaatt caggaaaaac gattttgatc gtccatcatg atcttcataa
    12481 agtagaagaa tatttcgatg aggttattct tttaaataaa cagctgatcg cttccggtcc
    12541 agtacaagaa gtctttacat cgaagaacct tcaattggcc tatggtgaga tcatcagaca
    12601 tttagtaaaa ggaggagaga aaaaatgatc caatccttta ttgatggatt aatgaattat
    12661 caattcctgc aatacgcttt ggtcacttcc atgcttgtag gacttgcttc tggagtcatc
    12721 ggttctttta ttattttacg agggatgtct ttgatgggag acgctatctc tcacgcagtt
    12781 cttccaggtg tagccatttc ttatatgttt ggttttagtt acatctttgg agccactgct
    12841 tttgggatgc taacagcagc agtgattggt ttcgtgactc agcggagccg attgaaaaat
    12901 gatacagcga ttggaattgt ctttagctcc ttttttgcat tagggattat cttgatttca
    12961 tatgcccaga gtgcgacaga tttgtatcat attttatttg ggaatgtctt agctgttagg
    13021 gaaagcgatt tgttgctgac agcactcgtt agtggaatcg tcttgatatt tgtctttttc
    13081 ttttataaag aattgaagat cacatcattc gatccgacaa tggcaaaagc ttatgggctg
    13141 aacacatcgc tgatccatta tcttttgatg ttctttttga cattagtggc tgtagtcagt
    13201 ttacagacag taggaacaat cttggtgatt gccatgttga tcacaccagc cgccacggct
    13261 tacttgctaa cgaaccattt actgaaaatg atcattacag ctgcaggaat cggtatgcta
    13321 agtgcagttg tcggtgtgtt tttcagttat agttacaatt ggccatcagg agctacgatc
    13381 gtgttagcat gtaccgcatt ttttatcctt gcttttttat tttctccaac aaaaggaatt
    13441 ttatttcaga aaggaagcag atagatgaaa acaaaaaaat cactcttttt aatcttggca
    13501 gttagtttct tggtattagc cggttgcgga aagcaggctt ctgaccaagc ggatgaagga
    13561 agtaaagaaa agttgtcagt cgtggctacc aattcgatct tggcggacat ggcaaaagag
    13621 gtcggaacag atcaaataga tatccacagt atcgtaccag tcggaacaga tccgcatgaa
    13681 tatgaagtac taccagaaga catcaaaaag gcaagcgatg cagatgttat tttatacaac
    13741 ggtttgaatc ttgaaacagg taacagctgg ttcgataact tgatggaaac ggctaaaaaa
    13801 gaagaaggaa aagattattt tgcagttagc aaaaatgtag aacctcttta tttaactagc
    13861 ggtgaagaac atacaaaagc agatccccat gcttggctag acctatctaa cggaataaaa
    13921 tatgtggagg aaatcgcacg tatattctct gaaaaagatg cagaaaatgc gacactctat
    13981 aaaaaaaatg cagaagcata tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa
    14041 agttttgctt ctatcgaaga gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag
    14101 tatttttcac gagcatatga tttgccagca gcttatatat gggagatcaa tacagaaagt
    14161 caaggtacgc ctgatcaaat gaaagcgatc attgatcaga taagagcaaa agaagtaccg
    14221 gttttattcg tggaaaccag tgtagactca agaagcatgg aacgggtagc aaaagaaacg
    14281 ggtttgaaaa tctatgataa actgttcact gattccatag caaaagaagg agaacaagga
    14341 gattcttact atcagatgat gaaatggaat attgaaacga tccatgaagg tttgtcacaa
    14401 acaaaagaaa gctgaaaagt gcaaggtcta gcttgaggag cagaccttgc attttttata
    14461 agagatatga aaaaaaatta tgctgttttt cgtcgtacag ataccttttt tgaagaaatt
    14521 ccgttatagt tagagtaagg aaggaaggtg tggctatgaa ttttacacgt aaagataaat
    14581 atacgattaa agaagaagtc tttcgattga tcagccagca ggaaaatacc gacaaggatc
    14641 aagaccttca atttcttgaa gcagaattag ctaaattatt catagcaaat caaaatgaga
    14701 caacagaacc agatgaagag ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt
    14761 tagatgaaaa taccggtatc ggttattttg tgttcctttc tccagtcggc ttcacacctg
    14821 gcgtattagt taaacaaaac gacgaaataa ccgtcaaagc atggcctgca ttattttcag
    14881 tcatcaaagc agaaggagta atcaaaaaga tccagtcggc ctattataaa agtcaattta
    14941 ttgccaacag caaattaatg aatatgtttt ataatcgtct agtagaaatt gctaggcctg
    15001 ggaaaatccc gcaaacattc acgtacacac agcaaaaaaa tctctatgaa gaagtattgt
    15061 attcccgcga tctgtctgag ttttcgccat gggaagaggt aaggctccat gaactgttaa
    15121 gtacaaaacc agtttcattg aattttttta atgcattaga taacaaaaga aaattaattt
    15181 caacaaatta ttgggaagta tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg
    15241 ccgatcagca gatccagttt gtccagctga aaaaagaaaa actatattat gggttataca
    15301 gccgttaaca gcaagcgagg aagaccatga cattaaaaga agaaatcata caagaaagca
    15361 aacgattagg aattgacaaa atcgggttca ccacggcgga accttttgat tcattaaaag
    15421 aatcgttgga agaacaaaaa gcagctggcc atacttctgg ctttgaacat ccaaatatcg
    15481 atgagcgtct ttatccagaa atgacatttg atcagccgaa atcgatcatt tccattgcac
    15541 ttgcgtatcc tactaggatc catgaagaag tgcctaggga tgaaaaacgc gggcagtttg
    15601 ctcgtgcttc ttgggggatc gattaccatg atatactcag agaccgtttg aaccgattga
    15661 tcgaattcat ccaaacaaga gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc
    15721 cgcaagtaga tactggagaa ctgatcgatg tcgctgtagc acagcgagct ggtttaggat
    15781 ttattggacg aaacggattg ttgatcactg aagagttcgg ttcatttgtc tatcttggtg
    15841 agatcgttac taatattgta tttgagcctg ataaaccagg agaatttggt tgcggtgact
    15901 gtatgcgctg cgtgactgct tgtccgacaa aagcattgct tggtgacgga agaatgaatg
    15961 cccagcgatg tttgtcttat caaactcaga caaaagggat gatgccggaa gaatatcgta
    16021 aaaaaatgcg aaatgtcatt tatggatgtg atatctgcca gttagtctgc ccttacaatc
    16081 aaggaaaaga ttttcatttt catgaagaga tggaaccaaa agtagacgaa gtatatccaa
    16141 aattaaagcc aatgctttcg atgtctaata aagacttcaa acagcaattt gggcatttag
    16201 ctggttcatg gcgtgggaag aaacctttgc agagaaacgc attgattgct ttagctaatc
    16261 taggtgatcg aacggcattg cctgagattt cagaatgttt aacagatgtc cgccctgtca
    16321 tcagaggaac agcagcttgg gctattggac gtctaggaac aaaagaaccg gaaaaatggc
    16381 ttgatgtatt agatgaaata ttagctaaag aaacagaaga agtcgttata aacgaattgg
    16441 aacatgccca gaaattattg cggagaaagt taaaataaaa aaatagttaa cattattcaa
    16501 a
//
LOCUS       DAPCMU010000057        16460 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPCMU010000057 DAPCMU010000000
VERSION     DAPCMU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.059"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(29..727)
                     /locus_tag="RD744_001474"
     CDS             complement(29..727)
                     /locus_tag="RD744_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDU0557478.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="RD744_001475"
     CDS             complement(720..2072)
                     /locus_tag="RD744_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDU0557479.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="RD744_001476"
     CDS             complement(2295..3695)
                     /locus_tag="RD744_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDU0557480.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="RD744_001477"
     CDS             3856..4593
                     /locus_tag="RD744_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDU0557481.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="RD744_001478"
     CDS             4718..5197
                     /locus_tag="RD744_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDU0557482.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="RD744_001479"
     CDS             5194..5961
                     /locus_tag="RD744_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557483.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="RD744_001480"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="RD744_001480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDU0557484.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="RD744_001481"
     CDS             complement(6864..7466)
                     /locus_tag="RD744_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0557485.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="RD744_001482"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="RD744_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="RD744_001483"
     CDS             8147..8731
                     /locus_tag="RD744_001483"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDU0557486.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="RD744_001484"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="RD744_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDU0557487.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="RD744_001485"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="RD744_001485"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDU0557488.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="RD744_001486"
     CDS             10632..10811
                     /locus_tag="RD744_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557489.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="RD744_001487"
     CDS             10833..11858
                     /locus_tag="RD744_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDU0557490.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="RD744_001488"
     CDS             12033..13277
                     /locus_tag="RD744_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDU0557491.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="RD744_001489"
     CDS             13430..14377
                     /locus_tag="RD744_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0557492.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="RD744_001490"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="RD744_001490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDU0557493.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="RD744_001491"
     CDS             complement(15279..15521)
                     /locus_tag="RD744_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557494.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="RD744_001492"
     tRNA            15642..15714
                     /locus_tag="RD744_001492"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16426)
                     /locus_tag="RD744_001493"
     CDS             complement(15833..16426)
                     /locus_tag="RD744_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0557495.1"
                     /translation="MGTIVVVLGLLGLLFAPSIISIFSLTITGFIAFTLVFGKKDTKK
                     MFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINEAFSPLLFIILPIM
                     ILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNILHTVAHILLIQGV
                     ARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5611 a         2941 c         3313 g         4595 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa
//
LOCUS       DAPCMU010000058        16265 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPCMU010000058 DAPCMU010000000
VERSION     DAPCMU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.060"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(95..1030)
                     /gene="murB"
                     /locus_tag="RD744_001494"
     CDS             complement(95..1030)
                     /gene="murB"
                     /locus_tag="RD744_001494"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDU0557496.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1103..2023)
                     /locus_tag="RD744_001495"
     CDS             complement(1103..2023)
                     /locus_tag="RD744_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0557497.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2136..2888
                     /gene="xth"
                     /locus_tag="RD744_001496"
     CDS             2136..2888
                     /gene="xth"
                     /locus_tag="RD744_001496"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDU0557498.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3752..4285
                     /locus_tag="RD744_001497"
     CDS             3752..4285
                     /locus_tag="RD744_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDU0557499.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4775..5299)
                     /locus_tag="RD744_001498"
     CDS             complement(4775..5299)
                     /locus_tag="RD744_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557500.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5292..5765)
                     /gene="tsaE"
                     /locus_tag="RD744_001499"
     CDS             complement(5292..5765)
                     /gene="tsaE"
                     /locus_tag="RD744_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDU0557501.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5888..6868)
                     /gene="pta"
                     /locus_tag="RD744_001500"
     CDS             complement(5888..6868)
                     /gene="pta"
                     /locus_tag="RD744_001500"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDU0557502.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7021..7701)
                     /locus_tag="RD744_001501"
     CDS             complement(7021..7701)
                     /locus_tag="RD744_001501"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDU0557503.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7827..8696
                     /locus_tag="RD744_001502"
     CDS             7827..8696
                     /locus_tag="RD744_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0557504.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8722..9237
                     /locus_tag="RD744_001503"
     CDS             8722..9237
                     /locus_tag="RD744_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557505.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9284..9877)
                     /locus_tag="RD744_001504"
     CDS             complement(9284..9877)
                     /locus_tag="RD744_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557506.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9902..10081)
                     /locus_tag="RD744_001505"
     CDS             complement(9902..10081)
                     /locus_tag="RD744_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557507.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10158..10667)
                     /locus_tag="RD744_001506"
     CDS             complement(10158..10667)
                     /locus_tag="RD744_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557508.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10763..11143)
                     /locus_tag="RD744_001507"
     CDS             complement(10763..11143)
                     /locus_tag="RD744_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0557509.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11474..12223
                     /locus_tag="RD744_001508"
     CDS             11474..12223
                     /locus_tag="RD744_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDU0557510.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12407..13684)
                     /locus_tag="RD744_001509"
     CDS             complement(12407..13684)
                     /locus_tag="RD744_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557511.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13763..14407)
                     /gene="pcp"
                     /locus_tag="RD744_001510"
     CDS             complement(13763..14407)
                     /gene="pcp"
                     /locus_tag="RD744_001510"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDU0557512.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14606..15550)
                     /locus_tag="RD744_001511"
     CDS             complement(14606..15550)
                     /locus_tag="RD744_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDU0557513.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15552..16238)
                     /locus_tag="RD744_001512"
     CDS             complement(15552..16238)
                     /locus_tag="RD744_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDU0557514.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4680 a         3379 c         2880 g         5326 t
ORIGIN      
        1 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
       61 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      121 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      181 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      241 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      301 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      361 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      421 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      481 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      541 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      601 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      661 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      721 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      781 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      841 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      901 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      961 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1021 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1081 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1141 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1201 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1261 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcctcttga atgacaagct
     1321 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1381 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1441 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1501 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1561 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1621 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1681 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1741 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1801 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1861 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1921 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1981 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2041 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2101 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2161 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2221 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2281 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2341 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2401 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2461 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2521 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2581 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2641 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2701 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2761 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2821 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2881 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2941 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3001 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3061 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3121 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3181 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3241 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3301 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3361 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3421 caaatggcaa aatagttcct catattttgc tattttttga gctaataccg agggtcagct
     3481 gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa
     3541 atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca
     3601 tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa
     3661 atattacata aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt
     3721 tacagtaatt tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac
     3781 tgccaatcat cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca
     3841 gatcgttgat gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt
     3901 ccaaattcac ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg
     3961 gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt
     4021 tgatacaaag gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct
     4081 ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt
     4141 gaacactgtg tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga
     4201 tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt
     4261 gaaaaaactt gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag
     4321 cctgccgttt cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt
     4381 aaatattgtg actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt
     4441 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     4501 ttcatttggt tggacttatt accgcagatg caagcttttg gacaccgttt accaagaggt
     4561 tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggcaaaata gttcctcata
     4621 ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt tattcaaaaa
     4681 aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg ccttctcaaa
     4741 aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa ctcattttat
     4801 atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc gcttcgattt
     4861 ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga acgtcaagct
     4921 ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct agcataagag
     4981 tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct cccacgtgag
     5041 acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc ccttcataga
     5101 ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct tcttctaagg
     5161 tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc tccttattaa
     5221 cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc cgcaagatga
     5281 tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg tttgaaggtt
     5341 tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc tgtatcactt
     5401 ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag atttccccat
     5461 tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc tgctccgcta
     5521 gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact atattctcta
     5581 ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg ggcaattcct
     5641 ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt gtcacctggc
     5701 attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc tgatagttcg
     5761 atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg taatgaagtt
     5821 tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc tagttcgcgt
     5881 gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta tatacatctt
     5941 cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt agaattggtc
     6001 cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt cctgactgca
     6061 attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc gctttcagtt
     6121 gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc atgtattgtg
     6181 gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct ggcgatttcg
     6241 ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg aacaattctg
     6301 ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga ttcacgttga
     6361 tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg atcatcgctc
     6421 cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg cgaacagtgt
     6481 cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata tacgtaagca
     6541 tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg gatgcctttc
     6601 catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa ttatcagggc
     6661 tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt cttatttctt
     6721 cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg gcagctgcgc
     6781 ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg cgaacaattt
     6841 taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt atatgtatta
     6901 tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc aaaaatgctg
     6961 gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag cgctgtttta
     7021 ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa ggagcgcatc
     7081 attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata acggactggg
     7141 atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt tttcttgtgc
     7201 cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt tttcaatgat
     7261 cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc cttcccgaac
     7321 ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta gatttccatg
     7381 ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc ttaacgatgg
     7441 cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat tttctccatg
     7501 ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca attccaatgc
     7561 ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt tttttaaaaa
     7621 ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt cttgccaact
     7681 gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac tcacattttc
     7741 ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa actaggaaca
     7801 agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat atggacggca
     7861 cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata cgttttgcaa
     7921 atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa gctcgcgcag
     7981 ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca caggtatttg
     8041 ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca atctctgttt
     8101 tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat ggtctttatt
     8161 ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc catttacctc
     8221 accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta ttgcacatca
     8281 catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt ttaaaaatta
     8341 tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag atttctcttt
     8401 taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac cataaaaacg
     8461 ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct ttagaagaag
     8521 tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca ggagtcagtt
     8581 tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca acggacacaa
     8641 accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac ttgtagtttt
     8701 atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca gcttggacgt
     8761 gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat ggttggtcgc
     8821 tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct cgttgctcac
     8881 atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga gttcgaaaag
     8941 gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat tcgtagactt
     9001 cactggacag tccaaggtga tatctataat catcaatatc aaatccatca tttcaatcag
     9061 caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt attggatgtc
     9121 tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga ttactggctc
     9181 taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt aagttaaaaa
     9241 gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata ttcttttgca
     9301 tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag tcatggcgtt
     9361 cgtataattt tttagccttt ggattttttt catccacact tagaccgatg acggagcgat
     9421 tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg ccttgtcctc
     9481 taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac gtttctttat
     9541 caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt accagtggct
     9601 gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca actttaccgt
     9661 ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca gtgattgctt
     9721 cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc agttccatat
     9781 cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt ctcgcaaaac
     9841 gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa tcgcaaaatc
     9901 gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta acgattcttg
     9961 ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta tgttcgacta
    10021 cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc attttttcca
    10081 tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt gtaaaaaaga
    10141 gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc tccatctatt
    10201 aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc tttcactttt
    10261 gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg tttgaactct
    10321 tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt tcgtacggtt
    10381 gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa ctggtattca
    10441 tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat agcagatatg
    10501 ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga gatccgatat
    10561 tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt cataccggta
    10621 atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct tcattctcct
    10681 gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat gaaacgaaaa
    10741 aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg tctggatcaa
    10801 agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct gattcgatcc
    10861 ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa gcaagatgac
    10921 gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt ccaaaaatct
    10981 ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct ttgcggtaat
    11041 tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat ttctgataat
    11101 ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca ctcctattct
    11161 tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc agattgaaaa
    11221 aaatagctgt ttttcctttt tagtaagaaa aaaggcaaaa aaaacagaaa gtaggtcctc
    11281 caagacactc ttcctgttcc caatataata aatgaaagta agaaattata ttttcaagaa
    11341 aacaaacaga aaattatcat aacacaaatt atttataatt gaaagactag ttttttttca
    11401 agaaaagcat acaatagatt gtgttagtaa atacagaaca cactacattt agtgtcttaa
    11461 ggagtcctca tacatgaagt catattttca gtttttagct catcagctaa aaggctggcc
    11521 gcaacaaaat tattatcttt tctttttcag tcttggctgc caggtcatga ctcttgtcaa
    11581 tagtcccatc acttggctgt ccgttattac atttatcggt accacgcttg gtgtattgtg
    11641 tattttatcc atcaatgcgg caaaatcagt caacggtatt ttaggtattt tgtctgcgat
    11701 ttgttttatt atcgttgggt tttcagcaaa gaactattta agtatcggtg aacagattgc
    11761 ttatatgatc acattggata tccccgtatt gttaagtaaa gaatggaatc ataatatggc
    11821 ttcaaaaatc cgaaaattca ctggaaaaag ctggtttatc gcaatcatca gtactttcct
    11881 tgtctatctt gtttcaggat tcctgattgg tcgttataca gatgatcctc ggccgtgggt
    11941 agatgccatt gctttttcta ttagcttgac tgctggtatc atcagtttct tgcgttacaa
    12001 caaccaatat tactggtggt tagcttcagg gcttgctcaa atggtccttt ggtatatttc
    12061 atttacgcat ggatctgcaa gtttggcgat gttcgtgaac agctctgttt acttgctgaa
    12121 tgatgtcctt gcatttaccg tttcaccttg gtataaccaa aaagaacgtg aacgtatgca
    12181 aaaaatggaa gaagaatact acaacgcaca acaacacgca taaaaaaact gagcgtggga
    12241 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaatcagaag
    12301 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    12361 cttcttattt cttggagtta aacgcttctg tcccagcctc tattttttat agtaattcct
    12421 tcagatactt tcttgcctca tcattgaaac gcagtgattc ttctaacttc tgataatatt
    12481 ccaacgcttg tttctgagct tcagtaaaac gataatgttt tcctttccat ttgtcgattg
    12541 tctcttgcac atttccagat tggatatctt tcactactag ttgccatttt tcttttgaga
    12601 ctgtacggtc tttcttatca gcagattgta tagcttctcg ttctttacgc aatactttta
    12661 aaaaaccatt tctgactatc cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc
    12721 gacaatattg cttccaaata aatagataga tttttggttg gatgaatcgt tggtttttcg
    12781 caaaatattc catctgagca gtgacttctg gtgttcgttg acgttttgtc tttctcaaca
    12841 cggaaataaa tgtttcaaaa taagcattag gatgtactgg cacttcttca tatacgatgg
    12901 attgccaatc tttacgtaaa aatgcgcgat agttgatcaa gaattcttct acatccttta
    12961 ctaattcttc tgcaaagtcg tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc
    13021 cattgatgtg ttcacttatt ttcttgattt cattttccaa tgcttgataa tccgctatgt
    13081 aaatcggcat ttctacttgg cgaaattctg cgagccgctg agctaatttt atatccggta
    13141 aatatggctg tttcatccga cgattttggt acaagacaaa acagtatttt tgccatagtc
    13201 cttcaggaaa atcgatattc ttctgcttaa gccataataa ttcgtctaag gccagatcaa
    13261 cttttgtcat tccctgatgg atcgaagctg ccactgtcgg agagacatgc aggtgatcgg
    13321 caaagtggtt gatgcctagt tttaggatct ttccggtttt tggcgattta acgatgtatt
    13381 gcacttttaa ttctctacca cactcacaat acaatttttg ctggcttcgt tttgttcgaa
    13441 aaaacggatt gatcttgaag tcagaaaaca cccaatctgt tgcagctaaa taatgttctt
    13501 ccataaaacg agagctgatt agatatcggc ggtgcttatc gactagttcc cgctgttttt
    13561 tcgttaattt tgcatagatc tcccaacgtt gactgcttgt gtattgttta gaaattttga
    13621 tgggatagct tttgctgatt tcttttggaa accattcttc aaagacggat tgccattctt
    13681 ccacggctgt ttctttcctt tctaattaca aaaagctggc tactaacata acgttagcaa
    13741 ccagccagtg tttcttgcta aattagtgag ttgttccggc atttccggaa atatcttctt
    13801 tattttctac tgctgcttca actgcataag tcaaagaaga agcaatgtct tctaaagaca
    13861 tagaaggcgt acccactgga cgatcgatca cttgatcggg tgagaatggc acgtggatga
    13921 acccacctcg aattgccggg aattgtgttt cgatcaaatg aagcaaacga tacatgatat
    13981 cattgcagac aaatgttcct gctgtataag agatgtacgc tggtagacca tgatcttgta
    14041 cattttttgt cattgctttg attggcaatg aggtaaagta agcggctgga ccgttttctt
    14101 ctaaagccgt atcgaatggc tgatttcctt cattatcagg gattcttgct tctgctagat
    14161 tgattgcgac acgttcgaac gagactgctg accgcccacc tgcttgtccg acacagatca
    14221 cgacatctgg ttggtgctgt tggatggctt ctttgactac ttgactgctt ttttcgaata
    14281 cggtcggtac ttccactttg atgatttcag caccgctgat ttcatctggc atttttttta
    14341 ctgcttcata ggctggattc actttgtccc caccaaatgg atcaaaacct gttactagta
    14401 ctttcatctg ttgttcctcc gtccgattga tttaacatat ggttatcttt tatcatacca
    14461 ttttccttga acagcgtaaa taaaggaaac aatagtttaa ataacataac agatcaaact
    14521 taatatattc ttataaaaaa atagtgttcc actacaatgt tagaagaggt tgcgacaatt
    14581 attctgtcac aacccctctc ttctattatt taaatagaat catataagca atttggaaaa
    14641 ctagcataaa taacgcaata aacaactggt ttttgatcac tccgtatttg tctttcatct
    14701 ccagcatcgc tacaggcacg atattgaagt tagcagccat cggtgtcagc agggtcccac
    14761 aaaaaccgca agtcaatgca accattccta ttaacgctgg attcgctcca tgactaaata
    14821 cgaatggtgc accgatccca accgtcatca cggtgatggc cgcaaatgca ttccccatga
    14881 ttaccgtaaa tatcaccatg ccaagagcgt aaatgatgat gccgatgttc acatttcctt
    14941 ctggaacgat cgtacctact gcattggaaa tcactgtccc aactccagcg cttgtaaata
    15001 ccgcacctag agaagctaaa agcattggca acatgcttaa cggccctact gtgcccagca
    15061 tatctgctgc atcatctaaa aatgtacttg gtcgattatc tcgagaaaag aacatcaaga
    15121 ttagcatggc tgcaaaaaca ccgacgccca cgccgactaa agcacctaaa ttggtaaata
    15181 gagcaaacaa gatcgcaaag acaccgatac tcaaagctgg aataaaaatt ttcattccta
    15241 gtttgtctga cattttttcc atatattctt ttgatggaag ccggctttct cctttgctga
    15301 ctttcttagc aattgctggt atggtcatcg caaagatcaa gccaccgttc acgattgccg
    15361 gaagccatct tcctcctgca ataactaccc ctaagaccgt ccagaaaaag gctgacccat
    15421 aacgatgagg attggtttta tctgttaaat ttcttactcc agtgtaaatc aatacgagtc
    15481 ccatcacgat ataaaggatc tccagtaatt tatcgcctaa taaaatatca ggatttgtaa
    15541 aaaagtccat cttattgccc ctttcctgga taatatttct tcgctaattt cttttcttta
    15601 agatagaagt agacacttgc cacaactaaa gccgtgatag ccactggaac ttccactaaa
    15661 gcaagctgtc ccaatgttac gtcatatcct agatctttca gtgttgattg gactagcaac
    15721 gcccctgctc ctccgatgaa tagtacttgg ccaaagaacc agcagacatt ttccatagct
    15781 gaagccatac cttttagttc ttcttcatgc ttaggatgga cttctaaccc ttttgattcg
    15841 atggtcccca atgccatcgg taaaatgatt ggacgaacaa atccagcaac tccaccaaaa
    15901 ctcacgttaa aagccgcaaa gatcattcga aagatcccat aaatatctac aatcatacca
    15961 gcagatactt tcttgaatcg cttgatcaag gaagccgctg attctttcag accatttctt
    16021 tcaagcgttc ctgttgccaa cataatgatg ataaaaatcg ccatcccgcg attgtttaca
    16081 aaacttgtac ctaaaagttc taacattcct tgaatgccaa gaccgccagt caaagctgta
    16141 acaactaaag caaccattac gatcaatatc gagtctaatt taaatgcaaa cccgagaata
    16201 acaatcaaga cgcctaaaag gctaagataa ttttccatat attttcctcc ataaaatgac
    16261 aatat
//
LOCUS       DAPCMU010000059        15897 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPCMU010000059 DAPCMU010000000
VERSION     DAPCMU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.061"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            138..1475
                     /locus_tag="RD744_001513"
     CDS             138..1475
                     /locus_tag="RD744_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDU0557515.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="RD744_001514"
     CDS             1523..3652
                     /locus_tag="RD744_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDU0557516.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="RD744_001515"
     CDS             3627..4316
                     /locus_tag="RD744_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557517.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="RD744_001516"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="RD744_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5713)
                     /locus_tag="RD744_001517"
     CDS             complement(5546..5713)
                     /locus_tag="RD744_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557518.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="RD744_001518"
     CDS             complement(5790..6230)
                     /locus_tag="RD744_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDU0557519.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="RD744_001519"
     CDS             complement(6234..7079)
                     /locus_tag="RD744_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDU0557520.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="RD744_001520"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="RD744_001520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDU0557521.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="RD744_001521"
     CDS             complement(8703..9650)
                     /locus_tag="RD744_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDU0557522.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="RD744_001522"
     CDS             complement(9873..10223)
                     /locus_tag="RD744_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDU0557523.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="RD744_001523"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="RD744_001523"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDU0557524.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="RD744_001524"
     CDS             11646..11966
                     /locus_tag="RD744_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDU0557525.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="RD744_001525"
     CDS             11988..12452
                     /locus_tag="RD744_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0557526.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="RD744_001526"
     CDS             12657..13262
                     /locus_tag="RD744_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDU0557527.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="RD744_001527"
     CDS             complement(13301..14590)
                     /locus_tag="RD744_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDU0557528.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="RD744_001528"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="RD744_001528"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDU0557529.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15897
                     /locus_tag="RD744_001529"
                     /pseudo
     CDS             15700..15897
                     /locus_tag="RD744_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5009 a         2933 c         3244 g         4711 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaa
//
LOCUS       DAPCMU010000060        15678 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPCMU010000060 DAPCMU010000000
VERSION     DAPCMU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.062"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(237..603)
                     /gene="ssrA"
                     /locus_tag="RD744_001530"
     tmRNA           complement(237..603)
                     /gene="ssrA"
                     /locus_tag="RD744_001530"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(774..1547)
                     /locus_tag="RD744_001531"
     CDS             complement(774..1547)
                     /locus_tag="RD744_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0557530.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1691..2041
                     /locus_tag="RD744_001532"
     CDS             1691..2041
                     /locus_tag="RD744_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDU0557531.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2022..2738
                     /locus_tag="RD744_001533"
     CDS             2022..2738
                     /locus_tag="RD744_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557532.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2738..4186
                     /locus_tag="RD744_001534"
     CDS             2738..4186
                     /locus_tag="RD744_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0557533.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4256..4765)
                     /locus_tag="RD744_001535"
     CDS             complement(4256..4765)
                     /locus_tag="RD744_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557534.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4755..5480)
                     /locus_tag="RD744_001536"
     CDS             complement(4755..5480)
                     /locus_tag="RD744_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557535.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5703..7823)
                     /locus_tag="RD744_001537"
     CDS             complement(5703..7823)
                     /locus_tag="RD744_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDU0557536.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8053..9231
                     /locus_tag="RD744_001538"
     CDS             8053..9231
                     /locus_tag="RD744_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDU0557537.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9247..10005
                     /gene="aroD"
                     /locus_tag="RD744_001539"
     CDS             9247..10005
                     /gene="aroD"
                     /locus_tag="RD744_001539"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDU0557538.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10028..10513
                     /gene="ybaK"
                     /locus_tag="RD744_001540"
     CDS             10028..10513
                     /gene="ybaK"
                     /locus_tag="RD744_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDU0557539.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10584..11837)
                     /locus_tag="RD744_001541"
     CDS             complement(10584..11837)
                     /locus_tag="RD744_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0557540.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11955..13304
                     /gene="gdhA"
                     /locus_tag="RD744_001542"
     CDS             11955..13304
                     /gene="gdhA"
                     /locus_tag="RD744_001542"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDU0557541.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13416..14762
                     /locus_tag="RD744_001543"
     CDS             13416..14762
                     /locus_tag="RD744_001543"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDU0557542.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15070..15456)
                     /locus_tag="RD744_001544"
     CDS             complement(15070..15456)
                     /locus_tag="RD744_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0557543.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15505..>15678)
                     /locus_tag="RD744_001545"
     CDS             complement(15505..>15678)
                     /locus_tag="RD744_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDU0557544.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         5121 a         2807 c         3157 g         4593 t
ORIGIN      
        1 ccatcagcat aaaagttcac ctcaaaaaga gtgtacgaac aaaagttctt tttgtaaacc
       61 ttaaaataga ttttcctgaa tacgtattgg ggatttctaa atgtttgaat gtttttttga
      121 atgcaaaatc aatgcaaaaa attttgtatt tctctacttt tctctccttg accaaaataa
      181 aaaaacgttg atttaacaac gtttttcata catgtttatc tgtctcaacc atacatatat
      241 ggagacggcg ggagtcgaac ccgcgtccaa acacatcgcc acttaaaaat ctacgttcat
      301 agtcaactca tttaaggatt cactttacag cttgccgagt gacaggcatt ctgtatcgct
      361 agtctgatgt tctctttttc aacttacaga cggaaagttg aaacgtatcc cactaaagtt
      421 aggaccctta ctcgagcaca tgggcgatgc cgagaggatc tacgctaact gtttttaggc
      481 agctaaagcg taagagttgt tttcgttttt agcagttata tttaactgta acgtttttac
      541 gtagacgtaa cctacgaaac gcaattcaag ctcgactgtg cctgtcgaat ccgtaacgtc
      601 cccgttagct aacaagggtt acgtgtattt ttcatacaaa attagtatag catatattgg
      661 gataaattca ataaaaaagc gaatcatatc ccattattta tataataact gcccctttct
      721 taagaaaaaa taagcgattt tcttaagaaa agaggtagct tcaaaaaaag atcttaaatc
      781 agatcaaaat acttcaatgc gtggacaatt ccgccttctg tatttttctt tgtgataaag
      841 gtagaaagtt cttttaatcc gtcataggcg ttgcccatag cgattttgtt gtcgcaagct
      901 tccaacaagg caaaatcatt cggaccatcg ccaaatccaa aagtaggaac tttttccagc
      961 tgaagttctt tttgcagacg tttgacacct gccccttttg agattccttt cttcactgta
     1021 tcgatcgagt aaggaccatt ccgataaaaa gttagctcag gaaaatgttc gtgatagtat
     1081 tcgtcacctg attgagataa gatcagcaac atatttactg tgttttcttt aaagcctaaa
     1141 ggatcaattt ccggaacatc tgaatgaatg aagtcgtaag cattgatcat tgtttggcta
     1201 tgaccggtac accagatttt ctgatcgtta tagtaagaaa gttcatgacc gttcaactgt
     1261 acatgatcat acattttttg acattcttct gaagaaaatg tttgagaata gatttcttcg
     1321 ccgtctactc gaatgaaaga accattcatg gtgaccgcag aagtaatgcc cgcatcttcc
     1381 atgatagagt ggatttctgc ttctgtacgt cctgtagcga tgacggggag aacattgttt
     1441 tcttttaatg cgttcatagc ttttgccact tcgggtgtga tttttgattt cgcatctagt
     1501 aatgttccat caagatcaaa gaaagtgatt gcacgataat ttgacacaaa taaaactcct
     1561 tttttattgg aataagttta tcttaacaaa gaatgaacaa aagacaaacc tttcgtgaag
     1621 aattcctgaa catattggtt cagtcaacaa tttagagtat actataagaa gagaatttag
     1681 gagggaaaga atgatttttt tcagtattgg tatcttattc ttaataattg gcagtctttt
     1741 tcggattctg ccctcaaaag gaaatctgcc tttttatggg tatcattcgc ctcttgccgc
     1801 aaaaacagac gctcattggc ggctggctca aaagacaagc ggaaactggt tttttcttat
     1861 gggcttactg atggcattga tcggctatta tttaaagaca tctgggcata cgaattattt
     1921 tctgattgaa atgcttctgc tagtatttcc aattatgcct atttttatta tgacagaaaa
     1981 gaaattacag aaatatgacc tggaaactgg agggaacgat aatgaatatt ttaatgattg
     2041 aagataatca atctgtttct gaaatgatgc agatgttttt cttaaatgaa ggttgggaag
     2101 caactttcaa gtatgatgga aaagaagggc tcgatgcctt tttagaaaac ccacaacatt
     2161 gggatatgat tacactagac ctgaatttgc ccacactgga cggtatgcta gtaggaagag
     2221 agatccgcaa agtttcaagt actgtgccga ttatcatgct gacagctaga gattctgaaa
     2281 gcgatcaggt gatcggctta gagatggggg cagatgatta tgtcacaaaa cctttcagtc
     2341 ctctgacgct tattgctcga atgaaagctt tgcatcgccg tagtgagttg gcagaagcaa
     2401 gagaagaagc agcagtttca gatgatcatt ttgatatcga gactagtcat ttcaagatga
     2461 atacgaaaac acgagaagcg tatctggaca accggccaat tgaaggactg acgcctaaag
     2521 agttcgatct attgtttact ttagcaaaaa aaccacgtca agtattctct cgggagcagt
     2581 tgcttgaatt agtctgggat tatcagtact ttggtgatga acggacagtg gacgcccata
     2641 tcaagaaatt acgacaaaaa atcgaaaagg tcggaccaca agtcatccaa actgtttggg
     2701 gagtcggcta caaattcgat gattctggtg ttgcctaatg cgttatttgt atcagcaact
     2761 cttagctttt tgcggaatgg tcgcgttgat cttattgatc gttggagttt cttttacaca
     2821 actgacaaaa caaacgattg aagaaaacaa ttaccagcaa ctgtttggat atgctgaatc
     2881 agttgaaaag acaacgcaaa cctatgccga tacgctcccg cagtttagtc aagatcaggc
     2941 gtttcaaaat gcattgctgc tgacagagca agtactgaca gaacaaaatg tgaactttat
     3001 atttatcgat aaaaacgaac gtgtcattta tccgactaat acagctttac tgaattttac
     3061 cttaacagct gatcaatgga aaaatctgag gaatggacgt caagaaaaat tcacatcgaa
     3121 taaaaatatt ttaggacaag cacaggcgac ctcttacgca cttgtgccat ttaatttgaa
     3181 tcatgaattc tatggagcac ttgtagttag tcaacctgcg cgaaatatcg ataacagcgt
     3241 tcgtccaatt acactgaacc tgttcaaagg atttattttt tccagtatcg ttgcagttat
     3301 tgccagctat gcttttgcag cattccaagt taagcgtatc aatcggttaa ggaatgcgac
     3361 gaaagaagtg acgaatggta attttgatgt acagctcccc gtacatgaca aagatgaatt
     3421 tgatgagtta gcagatgatt tcaataaaat gacgaattca ttgaaagaat ctcaagcaga
     3481 aattgaagaa caagaaaatc gtcgaagaca atttatggca gatgcttctc acgagatgag
     3541 aacacctctt acgacaatca acggacttct ggaagggtta gaatacaatg caatccctga
     3601 aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc tgatccgttt
     3661 ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat caaatcagta tggtcattaa
     3721 aaaattcaat ggaacagaaa cattgaaaaa tattgttgca cagttagaag ctaaagcaga
     3781 agcagcagga gatacattga cattgaaagc cgttgatgat attgatgttt acgctgatta
     3841 tgaccgattt gtccaaatca tggtcaatat tattcaaaac gcgatacagt ttactgaaaa
     3901 cggccaaatc acgattactt tagaaaaagg ctatcttgaa acgatcatca cgattgaaga
     3961 tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt attataaagt
     4021 agatccttca agaaaaaata caaaatatgg tgaatcagga ttaggtttac caatcgtgca
     4081 gcaacttgta cgcttgcata aaggaaaatt agaagtagaa agcgaactag gaaaaggaac
     4141 aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa caagcggtat
     4201 aaaagatcat gaatcgatct tctataccgc ttgtttttac tataaaatga ttgatttagc
     4261 ccgcgggctc taaacgatca atataaggac tttgagcaat agtagcatca tgagtaaaga
     4321 gtaagatacc ttgattttct tcatgagcta aataagaaag caagtggagt agctcttgga
     4381 tctccaatac actaagttca tcaaatgtat cttcaataac aatattgttt cgcttggaaa
     4441 gcaaggcatg aagtaattgc agctttactt tttcaaaaga cgtcaactca gctgccgcac
     4501 aattaagcaa ggaaggagag atatcaagtt tttcttgata ggccaaacga tcttgtttga
     4561 tttctttttc cggtacatcc aacaggacat ggtctttaag attcagataa ggaaacatcg
     4621 gccaattttt ttggatgatc gcaaaattat ccagttttgc aacaagaacc tcttttttca
     4681 agtgctccaa tattttatgc tgtttttcga gaggacacaa aataacggtc aagtgttcag
     4741 catgaatcaa atattcaggt ttcattacgt ccaggcctcc tactttgcaa atgatggata
     4801 aaaaaagtga aagggaatag actaagaatg agacagcatc cactgagccc aataagagtt
     4861 tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat tttggaaatc gatggtaaaa
     4921 aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg aagtcaagat atcacggata
     4981 tctggcagtt cagtagtctt taagacaaga aaattgatcc tttgaagaag cgcttcataa
     5041 actgactgga agcttacgat ccacaataaa accaagatac aacaaagaaa gagaggaaag
     5101 aacagctgcc agaaaataaa aaggaataat ttggttttag aaagaccagt caggcgccaa
     5161 ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat aaaaaggaag
     5221 gcaaatagaa aaaataaact gaatatgcct tttatataaa atcggctagc taattctaaa
     5281 gccggaagaa gctctgtaga tgcagaagcg ccaataaatg aaagccgatc ttggacttga
     5341 ttggctaaag ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa aaaagcagaa
     5401 aagcttgaaa ataaacaata gagtattgtc tgctttttgt aataaattaa tcgagcagta
     5461 gcatagcgga ttattttcat acttttcacc tctataaaaa gtatagaaaa tgaatatgaa
     5521 taaagtatga aattccttta gtgaaatcac gaattaaaac cagaaaatat tttgtaaaaa
     5581 gcaagaggtt gtgaagaagc tgccctattc tattaccgta gatgcaagct tttgaacact
     5641 gtttattcga aaagaggagc agcgacaata attttgtcgc tgctcctttt aaatttattt
     5701 tgtcattcgg aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg cgttttataa
     5761 agatcaacag ttgattgtcc gttgtgttca ctaaagacac tagttgactt gtcaccaaga
     5821 gatttttcag cttcttttaa tttctctaag ccattcttgt agtcataatc actggtatca
     5881 attgggtcta atccactatt cgtatagaac cgaagaagat caccattatt gatttggtca
     5941 gaaacagcta actgtttatc aactttttct ttcagtgaat caatttcatt ctgtacttcc
     6001 tcagtaggtt cagtaatagc ttcaccagtt tcgttactat agatcgtacc accgtagtaa
     6061 gtatattttg gtgtaacaaa atcaccatca cggaaagaaa caagctgttc atgatctttc
     6121 gagaatagat cttgtcctaa ctgaatataa ttttttgtat ctacacctag taagtgaagt
     6181 aatgtaggca aggcatcaac ttgtccgccg taagtgtgat tgattccgcc ctgtgtttgt
     6241 ccagggatat ggatcatata agggacacgc tgcatttgtg cattgtcgta gtttgtccaa
     6301 gtagaacttg tttttccaac taattcagct agatttttat ttcttgaatt cgagacaccg
     6361 taatggtcac cgtaaaggac gatgactgaa ttatcataca agccgctttc tttcaaataa
     6421 ttgaagaatt cttgtacggc ggtatctaaa taattggctg tcgcaaagta accgttgatc
     6481 gtttcatcat ttgtattggc taacgggaat cctgcttcgt cattggtgaa ttttgaataa
     6541 ggataatggt tagatacagc aatgaatttt gaataaaatg gttgttgcag acgttccaag
     6601 tattgtacgg attgttggaa aaatggttta tcatgcaacc cgtattggaa cgaattgtca
     6661 tcattaacat cataataaga agaatcaaag aaataatcat atcctaaatt cttgtatgtc
     6721 tcgtttcgat tccagaatgt accagcgttc ccatggaata ccgcactagt gtaaccttgt
     6781 gtttgactta aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa ttgtgtgaac
     6841 aaggagcctt gattcaagcc aaacaatgaa ttttccatca gcgtttccgc atcgcttgtt
     6901 tttcctgctt ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt gctgtggtaa
     6961 agactattga tgaaaggtgt aacttcatgc tggagaccat tctcatctgt caatttataa
     7021 tcgatcagga attgctggaa gctttctaaa tggatataaa tcacattttt tcctttagcg
     7081 agcccgaaca aatcgtcatt tggtgctgca taatgttctt ttacataatt ttctacttct
     7141 ttcatatcat tgggacttgc ttctgcccgc acttggtttg tctggtaagt ttgaacaccg
     7201 tcataaacca tgaaagcatt caagccaaga tatttgacca aataatcacg agagaatgtt
     7261 cgagtcagca attctggacg atcagcttcc gccataaaaa gatttcctga gaaaatcata
     7321 actgctaatg tagagatcgc aaaagccatt cttgctcgga tcgggcgagg gtccaacttg
     7381 attttttttg caatcagcag aacagtcaag ataataaaat cgatccaata gatcacatca
     7441 tatgggcgaa acagtcgaat ggcactttct cccagaccac tcgctacttt cccagcacct
     7501 aaaatcgtgt tgatcgtaat gaagtccgtg aattctcgat aataagtgac attcgaaaaa
     7561 acaagcaatg tcaaaagaaa ataaatgacc atcatcgtga tataagcaac tctagggcgt
     7621 ctgatataaa gagcaattcc aagtaataac aatgtagttg caatagggtt gataaacaag
     7681 ataaagtatt gaatgccact ttcgattccg agatggaaat caactaaata ggcaaaaatc
     7741 gttttcaacc aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt attcaaaaag
     7801 gcaggtgttt ttatacattt caagataatc ttcctttctg cacttcttaa tcacagaagt
     7861 gtcatgaaac catgttacac agctcactta ttctcattta ttttactagt atgacaatat
     7921 agtgtcaatt gaaaccagaa gtttatgctg tttcattaag gttttcttta agcttcatga
     7981 aaaaatgagc agtaagacaa gtcctatgtt ttttgttata cttgggaaga gaaagctaaa
     8041 gcaggtgaag gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt gaaaaaagga
     8101 tatccactga tccaaaaaga agaccttgct catccccaaa aagaaaccac gcaatgggtg
     8161 gaattagttg attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa gcagaataag
     8221 ggaatcggtt gggtattgag ccagcagaac atcccatttg atcaatcttt ttttgaagaa
     8281 ttttttgtga agtcaaaaga aaaaagaact ttcttttttt cagatgattc tactaccgct
     8341 tttcgtatat tcaatggaga aggagacgga atcggcggtc tgattgttga ttattatgca
     8401 ggttatgctg ttttttcttg gtataacgaa acgttatatg cacatcgtga attattgatt
     8461 caagcttttc gtgaaatcta cccggaaatc gtaggaggct atgagaaaat tcgatttgaa
     8521 tcagtaaatc tgcctgagag ccagttcatt tacggaaaag aggcaccgga acccttgatc
     8581 gtaaaagaaa acggagtaaa atttgccgtt tatttaaatg aaggattaat gactgggatc
     8641 tttttagacc aaaaagaagt acggggatca ttagcagaag gtttcgcttc tgggaaaaca
     8701 gttttgaaca tgtttagtta taccggcgcc ttttctgttg ctgccgcaat gggaggggca
     8761 atcgaaacaa caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca ggaacagttt
     8821 gaagtgaatg gtttggatgt cgatcagcaa aaaatcattg ttatggacgt attcgagtat
     8881 ttcaaatacg ccaaaagaaa acaattttta tatgacatga ttgtattgga tccaccaagt
     8941 tttgccagaa ataagaaaaa agtctttcgc gtagcaaaag attatgggga actggtcaag
     9001 gatagtctgg atattttagc agaagacggg atacttattg cgtctacaaa tgctgcgaat
     9061 gtctcgatag aacaattcca aacaatgatt gaagaggaat tccatgccaa aaatgtttct
     9121 tttgaacaac ttgctttata tcgtctgcca aaagatttcc agacgacgga taccttttta
     9181 gaaggcaatt atctgaaagt gtttgtttat caaattaaaa ataaccatta gaaggagcga
     9241 tgcagaatgg tttgggtaaa gggattgaat ataggaagcg gtagaccaaa gatttgtgcg
     9301 cctcttatcg gggccgtccg agaggaagta ttacgtgaag cagttcttgc acgtgaagca
     9361 ggggccgatt tagtcgaatg gcgagtcgat cattatcgtg aagttttttc ttttgatgaa
     9421 gtagcagaaa cattggttct tatccatgag atgcttgatg gactgccgtt attatttacg
     9481 tttcgtacct tagctgaagg cggagaacaa gacatatcga tccgtcagta tcgcgacctt
     9541 tatgaacatg tgatacacac aggattagta gatatggtcg atatcgagtt gttcaaagta
     9601 gaaagtctag gaaaaagact gatagaggaa atcaagtctt taaacattcc tttaattatc
     9661 agtagtcatg attttaaaga aacaccagca gaccctgtct tgctttaccg attgaatgtt
     9721 atggaacatt ttggagccga tatcggaaaa ctggcagtaa ctcccaataa tgaacgagat
     9781 gttttgcggt tgatggagtt gactagacgg gcaaatgctt ttgtctccat gcctattatt
     9841 acgatgtcca tgggaaatct tggtaaaatt tctcgtctgg ctggcggaac gacgggttcc
     9901 gttatgacat ttggttcctt aagcagcgat tctacttctg ctccaggaca attaccggtc
     9961 aaagcattga agcaaattat tcaattatta aatgaaccgt tgtagaacaa gtttgaggtg
    10021 atattttttg gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg tcgaacaaaa
    10081 aaagattccc tatacagagc acgaatatga gtgggacgaa agccacctaa gtgcgtcatc
    10141 ggtcgctgaa cagcttccag aaagccaatc tcgtatattt aaaacactgg ttgctgtggg
    10201 caatgtgacg gggcctcttg ttgctgtgat accgggagaa gccgaattga atctaaaaaa
    10261 attagcaaaa gtttcaggga ataaaaaagt agaaatgctc catttaaaag atttggaagc
    10321 aacgaccgga tacatacgtg gtggctgctc gcctataggt atgaaaaaac tatttcctac
    10381 ttatttagat caaattgcag aaagctatga acaaatcatt gtctcggctg gcagaagagg
    10441 gctacagatg gagttagcac ctcaagatat ctgtgcatta acgagcggac aatttgcaga
    10501 tataaaacaa taaatcatac gtataaaaga aagaaggagg ccgggatgtt gtttgtccag
    10561 tctccttctt tcttttttat acattatgct ctttgcccgg cattcattcc tgctacatgc
    10621 cctgtagcga aagccgctgt gatattaaag ccgccagtat aaccattgac atccagtaat
    10681 tctcccgcaa aatacagacc atcaatgatt ttactctcca atgttttagg ataaatttcc
    10741 tttagagaaa cccctccgcc agttacaaat gatttctcaa taggaaaagt tttgttgatt
    10801 gatagcttaa actctttgca tagttgaaca aaatcttgaa tttgtttttg agaggcttgt
    10861 tgtcctgtca gatgatccat ttcaagccgc tcaagaaaga aaactaacaa tcgctcaggc
    10921 aatataccat gccaagcatt gaccaagttc tttttggttg ctttactttt ttctgtcaat
    10981 atatgaataa gctcttgttt ggtttgtgta gggaaacaat ccagagaaag tgtcactggt
    11041 tctcccgttt tctctaattc tttattgaca aaacttgaac aacgcagtgc agccggacca
    11101 gaaataccga aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac acgacctttt
    11161 ttatttaaaa cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt tttctcctga
    11221 atatacggtt catccgatat caagggggac tctgtcgcat aaagaggcgt caccgtgtga
    11281 cccactcgct tcacgatctt gtacccatct ccagtagaac cagtagatgg ataagtccgt
    11341 ccaccagttg ttaagataat ggcaggagca tggaactctt cgaattccgt gcgtataccg
    11401 taaattttat gttctttgtg gatcaatttc tctactcgag tagagaatag aagcgtcaca
    11461 tctagttctt tcagacgatt gagcaatgct tcgataatcg tttttgattt atttgttaca
    11521 ggaaacatcc gtccatgatc ctcttctttt aaatgtacgc cttgtgattc aaagaagttc
    11581 atgatatcga aattattcca ttgcgcaaac gtactatata aaaactttcc atttccagga
    11641 atatgtgcaa tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg accaccacct
    11701 gtcatcagta gtttttttcc tgcttttttg tttttttcga tcaagagtac gcgtgcgcct
    11761 tgttcagctg cactgatcgc tgccatcatc ccgcttgttc cagcgccgac tacgatcaca
    11821 tcatacagtt tttccattcc ctcacctcaa ggaaagtgta gcatatctga agaaagaaaa
    11881 cagaaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa
    11941 aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac
    12001 atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg
    12061 ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa
    12121 cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc
    12181 gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat
    12241 tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt
    12301 tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc
    12361 ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt
    12421 caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag
    12481 aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga
    12541 caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat
    12601 tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag
    12661 tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag
    12721 gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag
    12781 atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa
    12841 accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag
    12901 cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct
    12961 tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag
    13021 ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg
    13081 gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa
    13141 gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc
    13201 aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat
    13261 ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt
    13321 ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta
    13381 ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt
    13441 caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag
    13501 cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg
    13561 atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa
    13621 tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc
    13681 gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa
    13741 aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga
    13801 tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta
    13861 ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag
    13921 aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag
    13981 aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct
    14041 ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat
    14101 tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg
    14161 aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt
    14221 tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg
    14281 gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg
    14341 atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg
    14401 tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg
    14461 gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa
    14521 cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg
    14581 atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt
    14641 acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt
    14701 tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt
    14761 aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa
    14821 tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg
    14881 ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct
    14941 ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt
    15001 ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat
    15061 attcttagct taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt
    15121 aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc
    15181 ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct
    15241 tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc
    15301 cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata
    15361 atctttacct acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat
    15421 caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta
    15481 ttatacccgt ggtgctagtt aattttaata tcctaaagaa tatctcaaag ttccttcaaa
    15541 acgtcgttgt gctcgactta atagaacact gtaagccagt aatatgcttt caaatctact
    15601 ctctagccct ttaacagaac gtgagttgaa attttgacaa cctaattttt ctaaggaaga
    15661 aaaaacagtc tcaactgt
//
LOCUS       DAPCMU010000061        15544 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPCMU010000061 DAPCMU010000000
VERSION     DAPCMU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.063"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(64..927)
                     /locus_tag="RD744_001546"
     CDS             complement(64..927)
                     /locus_tag="RD744_001546"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDU0557545.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="RD744_001547"
     CDS             complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="RD744_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDU0557546.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3783..4589
                     /gene="proC"
                     /locus_tag="RD744_001548"
     CDS             3783..4589
                     /gene="proC"
                     /locus_tag="RD744_001548"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDU0557547.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4668..6224)
                     /gene="rny"
                     /locus_tag="RD744_001549"
     CDS             complement(4668..6224)
                     /gene="rny"
                     /locus_tag="RD744_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDU0557548.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6635..7684)
                     /gene="recA"
                     /locus_tag="RD744_001550"
     CDS             complement(6635..7684)
                     /gene="recA"
                     /locus_tag="RD744_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDU0557549.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(7793..9034)
                     /locus_tag="RD744_001551"
     CDS             complement(7793..9034)
                     /locus_tag="RD744_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDU0557550.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(9274..9852)
                     /gene="pgsA"
                     /locus_tag="RD744_001552"
     CDS             complement(9274..9852)
                     /gene="pgsA"
                     /locus_tag="RD744_001552"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDU0557551.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(10061..10984)
                     /locus_tag="RD744_001553"
     CDS             complement(10061..10984)
                     /locus_tag="RD744_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557552.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(11723..13009)
                     /locus_tag="RD744_001554"
     CDS             complement(11723..13009)
                     /locus_tag="RD744_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDU0557553.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(13002..14270)
                     /locus_tag="RD744_001555"
     CDS             complement(13002..14270)
                     /locus_tag="RD744_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDU0557554.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            14522..14983
                     /gene="mscL"
                     /locus_tag="RD744_001556"
     CDS             14522..14983
                     /gene="mscL"
                     /locus_tag="RD744_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDU0557555.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(15043..15489)
                     /locus_tag="RD744_001557"
     CDS             complement(15043..15489)
                     /locus_tag="RD744_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557556.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         4412 a         3391 c         2726 g         5015 t
ORIGIN      
        1 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
       61 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
      121 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
      181 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
      241 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
      301 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
      361 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
      421 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
      481 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
      541 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
      601 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
      661 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
      721 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
      781 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
      841 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
      901 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
      961 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
     1021 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
     1081 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
     1141 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
     1201 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
     1261 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
     1321 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
     1381 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
     1441 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
     1501 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
     1561 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
     1621 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
     1681 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
     1741 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
     1801 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
     1861 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
     1921 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
     1981 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
     2041 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
     2101 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
     2161 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
     2221 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
     2281 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
     2341 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
     2401 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
     2461 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
     2521 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
     2581 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
     2641 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
     2701 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
     2761 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa
     2821 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
     2881 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
     2941 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
     3001 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
     3061 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
     3121 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
     3181 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
     3241 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
     3301 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
     3361 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
     3421 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
     3481 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
     3541 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
     3601 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
     3661 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
     3721 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
     3781 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
     3841 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
     3901 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
     3961 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
     4021 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
     4081 tagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
     4141 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
     4201 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
     4261 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
     4321 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
     4381 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
     4441 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
     4501 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
     4561 aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa
     4621 aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa
     4681 tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct
     4741 tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct
     4801 tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca
     4861 ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat
     4921 gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca
     4981 gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact
     5041 gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc
     5101 tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata
     5161 tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc
     5221 aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct
     5281 ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt
     5341 tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt
     5401 ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat
     5461 ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc
     5521 tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc
     5581 ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc
     5641 gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat
     5701 tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca
     5761 tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca
     5821 atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct
     5881 tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc
     5941 gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt actttctctc
     6001 agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct
     6061 tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat
     6121 tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga
     6181 ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata
     6241 ctatcttttt catttttatc atctatacag cttctatgat aaattattct aaactcgtca
     6301 atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta
     6361 ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat
     6421 ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt
     6481 ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa
     6541 ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac
     6601 tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt
     6661 cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa
     6721 ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt
     6781 cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa
     6841 tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca
     6901 tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct
     6961 ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca
     7021 aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa
     7081 acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt
     7141 tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat
     7201 gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga
     7261 ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt
     7321 ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat
     7381 attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta
     7441 cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt
     7501 cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc
     7561 cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt
     7621 taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg
     7681 ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat
     7741 atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc
     7801 ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag
     7861 cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga
     7921 tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag
     7981 ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt
     8041 ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt
     8101 ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt
     8161 cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct
     8221 tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat
     8281 aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc
     8341 ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat
     8401 atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc
     8461 gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa
     8521 aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg
     8581 gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc
     8641 ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt
     8701 tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat
     8761 tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg
     8821 tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca
     8881 actcttctaa acgctgctga ttatctccta caactgtttg ataataaact tcgataccta
     8941 gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa
     9001 gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata
     9061 cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg
     9121 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
     9181 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
     9241 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
     9301 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
     9361 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
     9421 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
     9481 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
     9541 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
     9601 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
     9661 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
     9721 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
     9781 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
     9841 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
     9901 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
     9961 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
    10021 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
    10081 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
    10141 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
    10201 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
    10261 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
    10321 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
    10381 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
    10441 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
    10501 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
    10561 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
    10621 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
    10681 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
    10741 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
    10801 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
    10861 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
    10921 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
    10981 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
    11041 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
    11101 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
    11161 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
    11221 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
    11281 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
    11341 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
    11401 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
    11461 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    11521 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
    11581 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
    11641 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
    11701 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
    11761 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
    11821 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
    11881 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
    11941 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
    12001 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
    12061 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
    12121 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
    12181 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
    12241 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
    12301 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
    12361 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
    12421 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
    12481 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
    12541 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
    12601 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
    12661 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
    12721 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
    12781 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
    12841 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
    12901 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
    12961 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
    13021 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
    13081 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
    13141 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
    13201 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
    13261 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
    13321 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
    13381 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
    13441 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
    13501 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
    13561 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
    13621 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
    13681 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
    13741 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
    13801 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
    13861 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
    13921 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
    13981 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
    14041 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
    14101 tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
    14161 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
    14221 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
    14281 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
    14341 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
    14401 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
    14461 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
    14521 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
    14581 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
    14641 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
    14701 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
    14761 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
    14821 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
    14881 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
    14941 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
    15001 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
    15061 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
    15121 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
    15181 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
    15241 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
    15301 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc
    15361 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
    15421 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
    15481 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
    15541 aaaa
//
LOCUS       DAPCMU010000062        15127 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPCMU010000062 DAPCMU010000000
VERSION     DAPCMU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.064"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            142..1977
                     /locus_tag="RD744_001558"
     CDS             142..1977
                     /locus_tag="RD744_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDU0557557.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2037..2753)
                     /locus_tag="RD744_001559"
     CDS             complement(2037..2753)
                     /locus_tag="RD744_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDU0557558.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2980..3522)
                     /locus_tag="RD744_001560"
     CDS             complement(2980..3522)
                     /locus_tag="RD744_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDU0557559.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3527..3754)
                     /locus_tag="RD744_001561"
     CDS             complement(3527..3754)
                     /locus_tag="RD744_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDU0557560.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3833..5713)
                     /locus_tag="RD744_001562"
     CDS             complement(3833..5713)
                     /locus_tag="RD744_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDU0557561.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5902..6516)
                     /locus_tag="RD744_001563"
     CDS             complement(5902..6516)
                     /locus_tag="RD744_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557562.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6658..7218
                     /locus_tag="RD744_001564"
     CDS             6658..7218
                     /locus_tag="RD744_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0557563.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7523..7837)
                     /locus_tag="RD744_001565"
                     /pseudo
     CDS             complement(7523..7837)
                     /locus_tag="RD744_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(8091..9726)
                     /locus_tag="RD744_001566"
                     /pseudo
     CDS             complement(8091..9726)
                     /locus_tag="RD744_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10165..11034)
                     /locus_tag="RD744_001567"
     CDS             complement(10165..11034)
                     /locus_tag="RD744_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0557564.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(11054..12175)
                     /locus_tag="RD744_001568"
     CDS             complement(11054..12175)
                     /locus_tag="RD744_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDU0557565.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(12177..12572)
                     /locus_tag="RD744_001569"
     CDS             complement(12177..12572)
                     /locus_tag="RD744_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0557566.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(12591..13397)
                     /locus_tag="RD744_001570"
     CDS             complement(12591..13397)
                     /locus_tag="RD744_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0557567.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(13390..14178)
                     /locus_tag="RD744_001571"
     CDS             complement(13390..14178)
                     /locus_tag="RD744_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557568.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(14192..14668)
                     /locus_tag="RD744_001572"
     CDS             complement(14192..14668)
                     /locus_tag="RD744_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0557569.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14740..>15127)
                     /locus_tag="RD744_001573"
     CDS             complement(14740..>15127)
                     /locus_tag="RD744_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0557570.1"
                     /translation="FNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYI
                     LQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSF
                     SVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         4731 a         3055 c         2358 g         4983 t
ORIGIN      
        1 cgtttcattc atcgtttcat cgaatacttc gcttttgatt cgattgaaca attaactcag
       61 cgttggaaaa aaagaattca ttggcttttt gttcataaaa gttatttttt agttgcagca
      121 tttttttact gctttacctt cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa
      181 gaaactatcc aggttgcctt aaaaatattt actcagcgac aagcacccat gatcctgaca
      241 atcatcatta ttatgggatt tttcttctta ctattgttga tttccaatcg tttttggtac
      301 gccttgactg ctaccttgat cattaactta ctgctcacta tttcgacagt aatcaaaatg
      361 gaaatgcggg aagagcctgt ctttccttca gatttaaaaa tgctgaccgg attgtcagaa
      421 ctattatcga tggttagccc agtcttatta atcgtgggcg gattgatcct tctcctatta
      481 ttgattacta gtattattgt acaacgtaga ctacaaaaac agtattcctt gaagattcat
      541 tggaaacgtc gatttatcag tatcgctgta ttattaggat gtttttcggg tgtctttttc
      601 attaatcaca aaaattcgcc ttctttcttg ctgtttaatt tatttaaagt aaacaagact
      661 ttctttaacc agcaaggcgc cgtaaaagat aacggaccag tcattcaatt cctaaacaat
      721 atcgacatta aaatcatgga agaacctgct ggctattcga aagaaaaaat taatggaata
      781 atgaaaaagt acaacacaaa ggcagatgaa atcaaccaaa caagaagtga ctggttaaac
      841 aatcagacga tcgttctcaa tttaagtgaa agcttcagtg atcctgcacg tgtccctaat
      901 ctgacagtac ctacagaccc tattccttac atcaaaagca ttataacaga aggaacagga
      961 ggattgatgt tatccgtcgg ttatggtggc ggaacagcta acatggaatg ggaaggactg
     1021 acaggcttga gtatcagtaa cctgtctgct tcgttagtta caccttatac ccagctagtg
     1081 gagcgtcaga aaataagtcc aaatattacc gatctattcg atgaaaaaat agccatccat
     1141 ccttttacag ctgctcttta tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg
     1201 ttctattatt tagacagtcc ggataaatta acttatacgg ataaaataga acaaagccca
     1261 cgcatatcgg atgattcagc atacaaagaa accttgaaag ctttacaaag caatacggaa
     1321 acgtcacaat tcatccaatt atccactatg caaaaccata tgccttatga tgattactat
     1381 agtgaattga actacactgc tgaaggaact gctgtcattc ctaatagaag aaaagaatta
     1441 caaacttata tgcaaggcct tcactatacc gatacagcag taaaaaaatt catccaagaa
     1501 atcgacaaaa tcgataaacc gattaccttt gttttttatg gtgaccattt acccgcaatc
     1561 tatagtggga atgattcgaa aaaatatgga ttagaacagc accagacaga ttatttcatt
     1621 tataacaaca agtatagtcg tgaacaagca gctacgttaa ataaagaagt tgttgctcct
     1681 aataactttg gtgctatggc tcttgagcat gcgaatatca aagtaactcc ttactatgcg
     1741 ttgttgacag aagtatctaa tcatctacct gcattaacaa ttgatcctac agaaagcttg
     1801 tctaatcgtt ttaatggaaa acaggtcttt gttacagagg atagtcaaat tatccaagaa
     1861 aaaaatttaa ctgagaaaca aaaagaaatt ctagaagatt atcgtctgat acaatatgat
     1921 cttgttgcag gtaaccagta cagtgcaaca tgggcgacac aaaaaataaa agactaacaa
     1981 ataaactgag caaggggttg tgataaaaga catgtcacaa ccccttgttt atctcattaa
     2041 tttgcttgtc cataaacgat ttgattaaag ccatttttca attcattatt cgctgtctcg
     2101 ttgaagtaaa gataattatc gggattcccc tgattttgtt gtgtcaccaa tacttttggc
     2161 tgattttgct gaaacccgaa tagatagacg tgctttttca gatatggttc tgtctctgca
     2221 tcggaataga cggctgtgat ttgatagtct gcttgacctg tcccactttc tgaccattct
     2281 gcagaaacag gcactccacc aatagccatc ttccactccc cgttcaaaat agcttgaggg
     2341 actttaagac catataagtc cacactcatt tgattcgtat aacttttgta ttgttgtccc
     2401 attgtttggc cccatttggt cataaatgcc tctaattgtg cggatttgtc tgcattccac
     2461 agtgtttgtt caacttgttc gctgctttct gaagaggaag atgaagcctc tacagaagaa
     2521 cttgaagtag atgtttgagt tgtttcagtc gttttggaag actctgacga agtagaggta
     2581 tctgctgttt ctgtacttgc tgcagttgac ttgctctctg tactagttgt tgtagtagtt
     2641 gcaactgttg ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta
     2701 caaccagcta acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa
     2761 actcctttcg attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat
     2821 ttttcgtatt ttctcatcaa tctttcaaat cttctaaaaa ctgagaagca cccgctgatg
     2881 ttgtctgtaa tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta
     2941 cctcacgctt tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc
     3001 atccaaatct tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt
     3061 attgaaactt aaaagttcaa caagcaattg ttgcaagcca tatttttctt ctttttcagc
     3121 taatttgatt gcccgtgtga taaagacatt ttggaaatca aaatcttttg caaagttttc
     3181 caacattgtt gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg
     3241 catgacttct tctaaagtag acggaacgat ttcattttca atcactaact catgagagat
     3301 tttattgaac catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg
     3361 cgaagatgta cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa
     3421 atttgctaat acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc
     3481 ttgttttgtt tcttgaacaa atttttcttc cactatattc attgatttac gcctctttta
     3541 cttttgctga ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt
     3601 ggacttcatc tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat
     3661 tgcttacccc aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag
     3721 ctagtgtatc taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga
     3781 tagtcttatt ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt
     3841 tttcttcttt gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca
     3901 agatactagc ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc
     3961 cgttgagtaa aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa
     4021 cagttttctt agccaagcgg taggcatgaa ccaacgattc aaaattagat tgcatcaaga
     4081 caacgtcact cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag
     4141 ccgcagccaa agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt
     4201 gttcttggta ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt
     4261 catcaatgcc taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca
     4321 tcactaactg ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc
     4381 gaatcacatc tgacacacct atgatttgag tgattttttt gtcgattgct acaataatga
     4441 ccgagctgcc tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtgagagaaa
     4501 gtttgtgagc ggcaatcaat ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag
     4561 ctgtgattcc ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa
     4621 tgtctttctc ctgagcataa tggatcacgg cgcgccccag aggatgatcg gataacgttt
     4681 ctaatcgagc cactaaagct aagctgtccc cttcatcctc tgtatagttc ttgatagtgg
     4741 atacagcagt agttcctttg gtcaatgtac cagttttatc aaagacaaaa gtatcgattt
     4801 tggaaaaagt atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac
     4861 cgattcctgc tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa
     4921 ccaaaacagt gattgctaaa cgaaaatcac gactaatcag accaacaacc aacgcaataa
     4981 gtaaaacggt tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt
     5041 tggtatcttg tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga
     5101 ccttagttgc ttcaatcgtc acgtagccat ctgctaaaat cgttccagag aagacttcat
     5161 ctccactgat tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcatacc
     5221 ctttcccgtc aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacga
     5281 tatcgccttc ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa
     5341 cactagcagt agatggggcc atctcggtca aagccttgat tgactcccgc gttttggtca
     5401 atgttttttg ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct
     5461 tgtattctcc gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga
     5521 taactttgac tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag
     5581 aagctatggc tagagcaatg ttatagaaca agacaaaacc agtaaatttt ccgataaaac
     5641 caaaaataat caacacagct gataaggcgg tgatatgatt tcgatgctct tggatccact
     5701 tttgtaattt catctttctc cctcttttcc tttcattctt acgagactaa gtatagatac
     5761 ttatccatca taaaaaattg accactatca agtttagtca aaaaggcggt tttcttttgg
     5821 tagaatacac tacaaaaaat agttagcaag aagactcact aaaagtgatc ttgccaacta
     5881 ttgtcgtttc tatcgaaatt ttcattgaat gattttcctg tacagcatac atcggagatc
     5941 tatttcttta tctaagattt ttatcttgct aattttttcc tttagtcttt caaatccacg
     6001 tcgttcatag aattgatacg tacagccact atctgtaaaa aggaatattt cttttccttt
     6061 ctctcttttt tcaaattctg ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg
     6121 atttgcagct aaaaaaatga tctctccatc tggcgaatgc ttctttaaat atgctgcaag
     6181 catttgttca tttgctgtct cgtaagctcc tttactttct ttagcaaata tattttgtaa
     6241 aaaatcgaat atcttaacat atatttgctg tccaaaagaa cgatgtttct ttttttctcc
     6301 ttttatctcc gccagcaaaa cacctgccaa ctcgtcctct gcatacaaag caagaatttg
     6361 agttgctcga ttcaactcca tatacaaaaa atatcttccg taaagattca gcagaagttc
     6421 attttccata tatctctcaa agttcattcc ttcaacagca aaatagatag cttttttgta
     6481 atctttcttt tctaagggtt taaaagttag tttcatcatt tcgcctcctc aacttcaatg
     6541 attgtgttat acttacatta taatagacac agtgtctact atctataatc aatcatctat
     6601 aagtttgtcg attaagcgac agctttgctt tgatatgtcg gataaggaga taggtaaatg
     6661 aaaaagacag atagacgcgt caaaaaaaca gaaaaagctt tggctgaaac actatccaca
     6721 ttattagtaa ataaaaagat tcaagcaatc actatacgcg aactaacaga aacggcagat
     6781 gtacatcgtt cgacttttta tacacattat aaggatatct atgatctata tgatcaatta
     6841 gaaagcaact tttttcggga tctaaatgaa attctctctt atgatccagc ccatagctat
     6901 gaggaactat acactcgctt gattgactat gtttacgcaa atactttttt atatcgctta
     6961 tttatcctca accatggtga tatttcgttt cagaagaaag ttaaacaagt tattgaagaa
     7021 aactatctga acatatggct atatgaagac aagaaaacga ttatcacaga agaaatgaga
     7081 ttctttacta cgtaccacgt ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc
     7141 aattacaagt actccaaaaa agaattatta gaactcttac agaaactgaa tgatcacata
     7201 gagaaaatca tgccttgaat aaaaacatac taagcttagt taagaagaac cgtaaccttc
     7261 tcgtataaca actgaatctt atttagccaa attgtttact gaagattttt tgagtaaaat
     7321 ttctataatt cctacttatt gaaaagtagt ttgtagtgct gcttctccct ctctaagtga
     7381 tatatcttgt tgtaccaatc aaattcacct catcattaat aaattacttt taggctaatt
     7441 tattacaact tataggttga agctgtaacc aatttatagt catattatat ttttatcatc
     7501 cagtttattg aattcttctc gtttacctca attcattttc aaggggaatc agctcatcat
     7561 ctaacatttt acgccatgct cgcttcattt cttgtgggta tttaaatggt ttcgcttctg
     7621 gatgtttggc tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa
     7681 ttggatcttc acctggcata tattgcagat tagaagcatg taattttttc tcagaaacaa
     7741 accactcatt ggaaaagaca tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt
     7801 taatgacaaa gatatcttca ttgctatcta gtgtaccttc atttctttgt gcttaaattc
     7861 aaagataatt ttctcaataa agatttttga ttccagtaag attcctttac tccttttaca
     7921 acatagcgtt gtcctttgtt ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc
     7981 caattctcta taaagaagat taagcttcat tattataata taaagtttaa atttggttta
     8041 ttacttactt tttcttattc tctagtaagc taatattgaa aaaaatgtaa tcagatcatt
     8101 atttttatat gttgtaactt cctgataaga aaactaatta aaagaaagag gtaactacat
     8161 aaactaatta aaaaagctaa cttcatagtt ggaggataaa tggaagaaac ttcaatgatt
     8221 gtttctcctt gtgttccttc aatcgacact agtcctatta tcaatctctc ttttttgtcc
     8281 atttactttt gcgactgttc cccaataatc aaatacagga agcgttattt tttgtccttt
     8341 agattcatgt actgtataga ctattttcga atccatcact tgataaggca tagtatgcca
     8401 ttccttgcca attcttactt catgattctg caaatgttct ttgtattgta atgcttccgt
     8461 gggtgcatag tcataaagtc ctccgggaag gagtgtcgtg taacgaataa gattttcttg
     8521 attcattacc aagtgatctc tctgagtgac aactttatgt tcatagtttg cttttgttgc
     8581 agcagataca gccaacagta ataaaacaat tgccgttata ttaactactt ttctttgctt
     8641 agctgatgtg ctcttcatga aaacgaacga taaaatcaac gagccaaaca gtacttgaaa
     8701 gcctaagatt cgccaaggaa attgaatcat ttgaatgggt gtattttgta ataaagacca
     8761 tggaaaaaat ttggtggata agagccaaga gactaccgca ctaaggtaaa tcactttttc
     8821 tttgcgatta aatcctttaa aaaagaagag tggacagatc aaagaaataa taccaatgat
     8881 tcctaccgta aaggttgtca ggtcattatt catcgcacca atcaaactat ccgagacatt
     8941 taatgcttgg tgttgtaagg tcgttttatc tggctcatta atcgattgaa ccatgatttg
     9001 tcggaacatc ggataccaaa agtatgctgt taaaagaatc gtcattccta ctgcctttga
     9061 caactccaca atcaaccgga tgctttcttt ttttcttgtt tcacttacta caaagccgcc
     9121 taagactagt aagccaacgg ctattacact cataaaagtg gaaagaacat gtgtataaat
     9181 gagtaagctc atactgactg ctaatggaat tgcacatggc tttttctcat aaatcagtgc
     9241 gtaagcataa tacataacta tcggtataac cgtaagtgca atcgcttcag caatagcaga
     9301 acgatgataa atatcaatcg tacggtaccc ataaaatgta tataaacagc taaatataaa
     9361 agccgcatcg attcttttta aaaattttag tccataatga aacatcagca tgatagtaca
     9421 aacattcaat agccaaacat ataagatata actaacgatt aaattattac ttatccaata
     9481 aaaaatgact gccggaaaaa aggtcaagta gggataaaaa taattcactc cgctcccata
     9541 atgattaaac gtcgtaaaat tgattggacc agaaaaaatt gaagataacc ctttgactct
     9601 agcaaaatgg aaggcaatat cccccgatgt agaaatagta tgaacatttc taaaataatt
     9661 gattgaataa agtccagcaa taattgctag gagaatggac aatattattt ttttctttac
     9721 ttgcattact taattatata ctcctttcat ggattgcttt atctagtata acttaaattt
     9781 taaataaatc gttatttatt tttactctca cttaaaaaag cactaaaatt ataagagagg
     9841 gaggtaatgg tctgaacacc aagaattgtt ccaacatcga attgcttaac taatgagttc
     9901 aaagcttaat tatgtggctc ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa
     9961 caaacatctt tcataaaaaa aacagccata tccgctttct tggatatgac tatttactac
    10021 tactttacta tctttcattc ttcttgtctc acttatgata aggcgctggg tatcatgagt
    10081 gaatgatatt gctgaataca aatagtccga ccacattaag ttgatcggac ttcataagca
    10141 ttttattgac taatactttt ttgcttaaga attgattcta acatcttttg ctaaagttaa
    10201 gcatcctata gttcctgcat tatctgctaa cttaggtgtg acaatgtatt cttcgatatt
    10261 tggcgtatcg acatagccgt taattagttc taaaaaacgt ctttgtactt tctttttcag
    10321 atgttcttgt tgcatgacac caccaccaaa aattataata tctggtgaaa gcatcagggt
    10381 cgtattacag gcacattgtg caatataatc cgcctcgatt tcccagaacg gatcttctat
    10441 agaaagatct tgtcccttgg ttcccgtacg catctcgata gcgggtccag atgccaaccc
    10501 ttcgagacaa tcatgatgaa aaggacaagt accagagaaa gtatctcctg aatgtctctt
    10561 aactaacgta tgccccattt ctggatggct aaacccttca acaaaaatcc catcttgtat
    10621 agctcctcca ccaattccag ttccaacagt gtaataaaca acactggata aaccttttcc
    10681 atgacctgaa acatactccc cgtaacaagc agcattcaca tcggttgtcc aggcaattgg
    10741 aatttcgaaa tgcttattca acgttccaac aaaatcaaaa tttttccagg cagtttttgg
    10801 agtagaggtg atatgtccat aggtctttga ttcacgatgg atatcaatcg gtccaaatga
    10861 ccctactcca atgctttcta attgttcttt atagtgatta aagaaatcaa ttaccaaact
    10921 cattgtttca ttcggtgtag ttgtaggaaa actcactctt tctaaaacct ttaaatcatc
    10981 attgccaatg gcacaaacaa atttagtccc acctgcttct attgagccat acatttctta
    11041 ctccaccgtc cttttatcta ggaattcgtt cttccctgtc taatagtttt tcgaaaaact
    11101 catgtggttc cttttggtac caataggcaa cagaagaaat atcgtcttga cgctcataaa
    11161 gcttgatgtc atcatttcct agggtttgga aagtaacacg taaattttct ttgaaactaa
    11221 tggggtcggg taaatgccag cggtacaacc catgcatcgg taaagcatca tcgccaaaac
    11281 catgaactgg atttggatta ggttttccag cactaaaata atcccgggtg tgatctcttt
    11341 tagattgaaa aggatagccc ataaatagcg tattaaaatt ttgagcatat gccctttctc
    11401 caaagttctt tttatggaaa gcccaagcgc cgccaaaata atcttctgct cctgtagatg
    11461 ttattgtagg gaaatcttca tctccatcta tataaaactt aaattctcct tccccccacc
    11521 agtatctttc caatgcgcac aaagcaaagt aggttcctac ataataccca gtgcctttga
    11581 ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat gggttcacgg ttccatttag
    11641 catgaaaata catcgcatca tccggtactt cagtcaatgc ataattaaat gtataaaaaa
    11701 tgtgcttaat aaactcagga tgttcattcg ttaaagtaat cttagctttt tttcgaaatg
    11761 gcatctcaaa atagcaattc atccctgatg ttggattcac tacgattggt aaggaattca
    11821 cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc taatggactt tctacagaag
    11881 gagtggattc atcatcccaa taaatacgca aaattaaatc acgtaagacg aaactcccag
    11941 cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa aattccaggg ccttccattt
    12001 ctgctaaagt aattgtctct ccagcattta tcggaacaga aggtgaccct tttctcccaa
    12061 ttcctagatg actcgctgtc attgccgctt tccctttttc accattttta ttttctggtg
    12121 aaatagttct actttcctct cccttgtgga gccatatatt atttaaacaa ttcattttat
    12181 tcgtcctcta tcctttcttc acttttttga ttattcatat agatgatttg ttgttgtgcc
    12241 tccgcaataa ttgctccaat atctaaatta tcatttccta actgcaaagc gattgacatt
    12301 gccaagggaa gattcattcc tgcaattacg tgcactctgt cagaaatata ggtaaaaaac
    12361 ttttgattta cacttcctcc agccatgtca gttaaaacaa ttatttcgtc tgtagtatcc
    12421 actaactctt taatttgttc atctatatca tcaatttctt cgacatatgc acataagcta
    12481 ctaatttgat tatgcatacc tgttaaaaac tctagtgtct ctttaaaacc ctcagctagt
    12541 attgaatggc tagcaatgat caatcttctc ataattcaaa tccttttcct ttacgctaat
    12601 attccaaatg cagccataac catcgcaaaa acaataacga gtagaatcaa aatggtcata
    12661 ctgatttttt tcttactgag tagataatat gctgcagcag taataattac tggaattaat
    12721 gttggtaaaa ttttgtccaa cattggttgg attttcatac taacttcacc aaaagtgaat
    12781 ttaagtggcg ttgaaacact gataactgaa gcaattaatg atccaactac tgttagcccc
    12841 aatactgaag cagcttcagt taaataatta atcttttcac cgtattcagt aacaatcttc
    12901 attccctgct tataaccaaa ttcaaagcct ctcattcgga aaaagaagat taccatgatt
    12961 aaaatgaacc ataaaaagat tcctacagga ttattttcta aagccatata agccgcaatt
    13021 gatcccatga tggttgggat catcgtcatc aaaagagagt ccccaatacc agcaattgga
    13081 cccatcgtac caactttaaa gtcattaatc gcatcttttg cttctaatcc ttgcttttcc
    13141 tccattgcaa cacaagcacc taaaataatg gctgccagcc aaggctgtgt attgtaatac
    13201 tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat ctttgtataa ttttcttaaa
    13261 ataggtgaca atgcgtaagt cacagatgca ccttgctgtg tttgataatt aaaggtacaa
    13321 actgacatca accaacgata gttcgctttc ctaagatcct ttttcgttac ttgatatttt
    13381 tcgctcattt tattcatcct ccccaataaa cccaacatca gaagctcccg cagaagcagt
    13441 gatttgtgca tctttttcag ctctatgata cacattaaat gctaaagcga atcctatgat
    13501 cgccgctcca ataataggta ccttcaaaaa agcggctaag aaaaatccaa caagtaaata
    13561 actaatgtat cgacctgtag gcataaagcg taacaacatc gcaataccaa cagcaggcaa
    13621 catttttccg gctaatgtta gcccatccat aaaccaatta ggtaaaacat ccaaaacgta
    13681 attaacagca gactgtccta aagtaacggc aataaatact ggaattgctg cagataaacc
    13741 aaagaaaatt ggccccaacc acaaaacact attcattttc ttaaattctc gtttattcgc
    13801 atattcctgt gatttgcgaa caacaaaacc atttaagatt ttgacaatta catctagttg
    13861 cacccctaac atacctacag gtagtcctat tgctaatccg gcttctactc caagctggct
    13921 accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc ggtacagatg agccaccaaa
    13981 accggcaact cctaatgaca tcaattgaac tgtaccgcca actatcaaac ctgtttttat
    14041 atcacccatc aaaattcctg tgaataatcc ccaaaaaact gcgtaattta caaagatcat
    14101 cggaatccca taataatcaa catgtttaat aaatgctaat atagtcagca taataatttg
    14161 aaaaatagta aaatgcatta tccttactcc tttatcctaa ttttgattga atttcctgtg
    14221 ccttatccgc cggaacaaat tgtgtaacaa tttctacacc agaatccaac aattttttat
    14281 acgtgctgat attttcatct gatgcatagg ctctagaaga tagctgtaat gcattatctt
    14341 taacaacagt accgccaaca attagtcggt caattctaat accgttatct actaaatgaa
    14401 ggattgtttc cggctctttc acgatgacaa atactttttc ctgatcgtat ttcccctttt
    14461 taaaattcgc aatcgctgtt tcttcagtga taattgatac agccattcca gcaggtctag
    14521 ctaaacgcat acttgatttt tggatgtcgt tatttgcgac agaatcatcg atcaccatca
    14581 ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat aatcccatgc aataaacggt
    14641 tatcaactct cgacataaca atactcataa atacttcctc ctttatcttt cattttctta
    14701 caagcaatta ccatgccaaa acaaaaagaa cttgataaat caagctcttt ttacataatc
    14761 ataaacgtat ttaacctcat gaatcgggat gtcgacacta taataatgtt caacgacact
    14821 gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc tcactcgttt cattgaaata
    14881 aatttcatct tccaaagtat tttgttttac taatcgttca attaagcaac taatgtgaac
    14941 atataatccg aaacacgtat tattatcaaa tgaaattcct agatcatttt gtaagatgta
    15001 tatagcatct gaaacccgtt ctaataattt agtcggattt aatatggtca attgctccat
    15061 taaattcgtt agactaaaat tcttcagaat attattgttc aaaactttca gttcattatc
    15121 attaaag
//
LOCUS       DAPCMU010000063        14883 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPCMU010000063 DAPCMU010000000
VERSION     DAPCMU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.065"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            21..851
                     /locus_tag="RD744_001574"
     CDS             21..851
                     /locus_tag="RD744_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDU0557571.1"
                     /translation="MIGLAILLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTEN
                     PGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIV
                     NKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGE
                     TAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWT
                     IWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE
                     "
     gene            966..1886
                     /gene="pstC"
                     /locus_tag="RD744_001575"
     CDS             966..1886
                     /gene="pstC"
                     /locus_tag="RD744_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDU0557572.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1886..2770
                     /gene="pstA"
                     /locus_tag="RD744_001576"
     CDS             1886..2770
                     /gene="pstA"
                     /locus_tag="RD744_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDU0557573.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2785..3591
                     /gene="pstB"
                     /locus_tag="RD744_001577"
     CDS             2785..3591
                     /gene="pstB"
                     /locus_tag="RD744_001577"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDU0557574.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3607..4365
                     /locus_tag="RD744_001578"
     CDS             3607..4365
                     /locus_tag="RD744_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDU0557575.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4378..5055
                     /gene="phoU"
                     /locus_tag="RD744_001579"
     CDS             4378..5055
                     /gene="phoU"
                     /locus_tag="RD744_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDU0557576.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5293..6324
                     /locus_tag="RD744_001580"
     CDS             5293..6324
                     /locus_tag="RD744_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDU0557577.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6321..7088
                     /locus_tag="RD744_001581"
     CDS             6321..7088
                     /locus_tag="RD744_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDU0557578.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7188..8756
                     /gene="liaX"
                     /locus_tag="RD744_001582"
     CDS             7188..8756
                     /gene="liaX"
                     /locus_tag="RD744_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDU0557579.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9053..9373
                     /locus_tag="RD744_001583"
     CDS             9053..9373
                     /locus_tag="RD744_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDU0557580.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9373..9723
                     /locus_tag="RD744_001584"
     CDS             9373..9723
                     /locus_tag="RD744_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDU0557581.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9890..10828
                     /locus_tag="RD744_001585"
     CDS             9890..10828
                     /locus_tag="RD744_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDU0557582.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10843..11673
                     /locus_tag="RD744_001586"
     CDS             10843..11673
                     /locus_tag="RD744_001586"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDU0557583.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11717..12742
                     /locus_tag="RD744_001587"
     CDS             11717..12742
                     /locus_tag="RD744_001587"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDU0557584.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12757..13695
                     /gene="galU"
                     /locus_tag="RD744_001588"
     CDS             12757..13695
                     /gene="galU"
                     /locus_tag="RD744_001588"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDU0557585.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13820..14260
                     /locus_tag="RD744_001589"
     CDS             13820..14260
                     /locus_tag="RD744_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDU0557586.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14263..14865
                     /locus_tag="RD744_001590"
     CDS             14263..14865
                     /locus_tag="RD744_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDU0557587.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         4887 a         2554 c         3231 g         4211 t
ORIGIN      
        1 aacttaaatt attcttacca ataataggat tagctattct tttatcaggc tgttcaaagt
       61 ggattgatcg aggagaatcc attacggctg ttggctcttc tgctttacag cctttagtag
      121 aaacagttgc tgaaacttac cagacagaaa atcctggaaa attcatcaat gtacaagggg
      181 gcggaagcgg aactggtttg tctcaagtac agtcaggagc agtcgacatc ggaaactctg
      241 atttgtttgc ggaagaaaaa tcggggatcg atgcctcagc attagtcgat catcgagtag
      301 cagttgttgg gatcactcct attgtgaaca aggatattgg cgtaaaagac atcagtatgg
      361 aaaacctgaa aaaactattt acaggcgaga tcaaaaactg gagtgaagtc ggtggaaaag
      421 atcagccagt agttatattg aaccgcgctt caggaagcgg aacccgtagc acgtttgaaa
      481 aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca agcggaatgg
      541 tgcgccagat cgttgccgac acacccggtg caatcagcta tgttgctttt tcttatgttt
      601 ccgatgatgt agatacgttg aatgttaacg gtgtagaacc gacagatgaa aatgtgacaa
      661 cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagatcat ccaaaaaaac
      721 tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataac atcgttggag
      781 aactaggtta tatccctgtt tctcatatga aagtcgaacg tgattggcaa ggaaatatcg
      841 ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca tgttattaaa
      901 accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt aagggggagt
      961 catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca aaaatggaac
     1021 agcgtggaaa attcatcagt tttttatgta tcgcgctgat cgtgttggtt gtattatcta
     1081 ttttttattt tgtagctagt aaaggattgg ccaccttttt tactgataag atcaatgtat
     1141 ttgattttct gtttggtaca gattggaatc caagccaaat cggagcaaat gggaaaccaa
     1201 tggtaggggc attgcctatg atcacaggtt cgtttattgt gaccttttta tctgctatcg
     1261 ttgccacgcc atttgccatt ggtgcagcag tattcatgac ggagatctct cctaaaaacg
     1321 gaacgaagat cttacagccg gtcatcgagc tgcttgtagg tattccttct gtcgtttatg
     1381 gttttatcgg tttatcagtt atcgtgcctt ttgtcagaag tatttttggc gggactgggt
     1441 ttggtatctt agctggtaca tttgtgttgt tcgttatgat cttgccgact gtcacaacga
     1501 tgactgttga tgcattaaag gcagtgccac ggcactatcg ggaagcttca ttagcgcttg
     1561 gtgccacaag atggcaaaca atctacaaag tcgtgctacg agcagccgtg cccgggattt
     1621 taacagcagt cgtttttggt atggctcgtg cgtttggtga agccttagct atccaaatgg
     1681 tcatcggtaa tgcagcattg atgccaacag gtcttactac tcctgcttct acattgacgt
     1741 ccatcttaac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat aatgtgttat
     1801 ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg atccgtatga
     1861 ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa ttgctacagg
     1921 tgttttatat atagtttcgg ggatcatcgt attgatttta gcagctttac tactttatat
     1981 cttagttcgg ggaataccgc acatttcatg gtccttcttg accgaacctt ccaaagccta
     2041 ccaagaaggc ggtggaatcg ggatccagct atttaactcg atttatttgc ttttgatcac
     2101 gatgatcatc agttttccta tttctttagg tgcgggtatc tatctttcag aatatgctgg
     2161 aaaaaactgg ctaacagatg tgattcgcac gtcgattgaa atcttgagct cgttgccttc
     2221 tgtcgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt atggtttctc
     2281 gattatttct ggtgcattgg ctttgactgt attcaacttg cctcttttga ctagaacagt
     2341 tgaggagtct ttacaagcgg tccattacac acaacgagaa gcaggattgg ccttaggttt
     2401 gtcacgctgg gaaacagtga taaaagtggt ggttccagaa gcgacaccag ggattttgac
     2461 tggtgtgatc ctaagttctg gaagaatttt tggtgaagca gctgcattga tctatacggc
     2521 tggacaaagt gcacctgcgt tagattttac gaactggaat ccattaagtg tttcaagccc
     2581 gatcagcatt ttccgtcaag ctgaaacatt agctgtccat atttggaaga tcaatacaga
     2641 aggcacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt tgattattgc
     2701 tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc gaaaaatgac
     2761 ttcagcctaa ggagaagaag caaaatgaaa aaatataact ggaatgaaac aaatgtcatc
     2821 accatgccaa aagagaaaga agtagcacta tatacggatg atctacatgt ctggtatggc
     2881 gacaatgaag caatcaaagg agtggatctg gaatttgaaa aaaataagat cactgcactg
     2941 attggtcctt ccggctgcgg aaaatctacg tatcttcgtt cgttgaatcg aatgaatgat
     3001 gggatcgcta atacgaaaat cactgggaaa atcatgtatc aaggcgtaga tattaatgcg
     3061 cctcaagtgg atgtttatga gatgcgcaaa cggattggca tggtttttca acgtcctaat
     3121 ccgttcagca agtcgattta tgacaatatt acatttgctt tgaaacagca cggcgaaaaa
     3181 aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagctgc cttgtgggac
     3241 caagtaaaag acagcttgga caaaagtgcc ttggctttat ctggcgggca acagcaacgt
     3301 ttatgtattg ctcgtgcgat cgcgatgaaa cctgatatct tgctgctaga cgagccggca
     3361 agtgctttgg accctatttc gacaggaacg gttgaagaaa cacttgtaaa cttgaaagaa
     3421 gactatacga tcatcatcgt gacgcataac atgcaacaag cagctcgtat cagcgactat
     3481 actgcctttt tctatttagg aaaagtaatg gaatatgaca aaactaacaa gatatttacc
     3541 agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta agacggaggc
     3601 ctcaacatga caaaagagat tatttcatct aaagatcttc atctgtatta tggaaagaaa
     3661 gaagcactaa aagggatcga tctaagcttc aatcaagggg aaatcactgc gatgatcggt
     3721 ccttctggtt gcggaaaatc cacttatctg cgttctttga accggatgaa tgacctgatt
     3781 ccgaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta tggtccaaaa
     3841 acagatgttg ttgaactaag aaaagaaatc ggcatggtat tccagcagcc aaacccattt
     3901 cctttttcta tctatgaaaa tgtcatctat ggattgaaat taaaagggga aaaagataaa
     3961 aaagtccttg atcaagtagt tgaagaaagc ctgaaggcag cgtctgtttg ggatgatgtg
     4021 aaagacaaat tgcataaaag tgctttatct ttatccggtg ggcagcagca gcgtgtatgt
     4081 atcgcacgtg ttttggcagt taatccggaa atcatcttat tggatgaacc aacaagtgcg
     4141 cttgatcctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag agagcggtat
     4201 acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga taagacggca
     4261 ttctttttag atggtcactt gatcgaatac aatgatacga agaagatatt catgaatcca
     4321 gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg aaacggaatg
     4381 ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta cgaaatgggg
     4441 atgatggtga gcagcgccgt gcacaagtca gttcgtgcat acatcaaaca cgataaaatc
     4501 ttagcccaag aagttatcga aaacgatata aatatcaata acatggaaac acgcttggag
     4561 aagaaaagtt ttgaaatgat cgcattgcaa cagccagtta caactgattt gcgtatgatc
     4621 attacggtca tgaaagctag ctcggattta gaacgtatgg ccgatcatgc ggtgtctgtt
     4681 gctaaatcca cgattcgcgt aaaagggcag acgagaatac cggaaatcga aaaagagatc
     4741 tccgatatgt ccgattatgt gaaaaaaatg gtggacaacg tcctagttgc ttatgtcaaa
     4801 acagacgaaa aagacgcacg tacgatcgcc aatatggatc aaagagtcaa tgactatttc
     4861 aatcgaatct ataatgcaac aatcaaaaat atgcaggaaa atcctgagac agtcatcagt
     4921 ggaacagact acttgaatgt tgccagttat ctagaacgga tcggagacta tgtgacaaac
     4981 atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgagttaaa tacgaaccac
     5041 aatgagattt tctaaactga agctgtgatt atgccgtttg tttaggaaaa gaggttgtga
     5101 aatcagcggt tgtgaaaaag ctgttctgca tcaagtaata agaacagtca aacaaaaaat
     5161 agctcctcat atttttcgtt tggctggact tattaccgca gatgcaagct tttgaacacc
     5221 gtttattcgg gaaagggttt gtgacaaaac atgtcacaaa cccttttcat catttcatag
     5281 aaaggataaa gaatgcaaaa aaaaattagc atcatcacta tattttttat ttgtgtgctt
     5341 acttcatggg ttattcagca tcaagccgct ttatatgaag tacctgtcat cgagatagaa
     5401 aaagtacgaa taaaagagga tcatcaattg attacaggaa aactgatgaa cacggatcaa
     5461 aaaggagaat cgatttattt atcggttcct tatcaaaaaa atcaaataga aacagcaaat
     5521 ataaaagcga gacaaatgta tctagtccaa atgcatggag atgaatggca gctagtagca
     5581 aaaaaaaatg atggctggct tttcttcttc acaagtatct tcattggcgc catgttgctt
     5641 attggtggaa aacagggtct atttgcttta ggaagtctca tcatcaatat cgttttactt
     5701 ttacttttgt tgctcatctt tactaaaaca gaagggattt ctctattaaa tatatctatc
     5761 ctgttcgtcg ttatagggat gattattagc atattggctt tagatggatg gaatagaagc
     5821 agtataatca aaatttcagc tgcagttacg agtgtttttt tagcattttt tatttgtctt
     5881 ttgacaatga atcattggaa agaccaagga ctgcgctatg aggaactgaa ttatctgaca
     5941 cgaccgtatc gttctgtttt tctttctagc gtactgattg gaacagctgg aggaacactc
     6001 gataccgtta tcacagtcat tgcgacatta gaagaattga ctaagcgaca accagaaata
     6061 acagcgaaac agttatggct ttcaggaagg caagtgggtc gagatgtgat tggctcgatg
     6121 accaatatcc ttttgttcgt ctatatcagt gggtcagttc catcgcttct tttatattta
     6181 ggaaatcaat ggtcgttcaa agaaacgata gaacagcatt tatcattgga atttttacgc
     6241 gtcattgctg gcagcttagg aatagtatta tctatcccta tctcggtcct cttttttctg
     6301 actgtaagga ggctgaaacg atgaatgtac ttattatact tgttgggatt ttagcaatca
     6361 gcgtgctact tattggcggt acgcaaggta tgtatattct tttaggctta ttcatcaatt
     6421 tagggatctt tttcctgttg ttattcggtt atcatcagaa gtggccgata ctggcattgt
     6481 cggtcatagg atttttattg attgctgtgg ttatcttatt ttttatcaac ggttataatc
     6541 taaaaatgcg tgcagctttt gtgagtatac tgatcttttt attttgtttc ctactattgc
     6601 ttcctatcac tgatttttta gcaatccaag gttttacatc ggtagaacta gaagaattat
     6661 ctggcttaga taagacttta gcgattgatt ttagattact tacgagatcg ttgcttctga
     6721 ttagtcttag tggtgctgtt ttagatgcga gtgtagcaat ttctagtggg acattcgaag
     6781 tatatcaggc aaatccgcaa ttatcttttt accaattgcg ccattctagt ttcgctattg
     6841 ctaaaaaaat attggcgtca acagtaatga cacttctttt tgcattcatg ggaagttctt
     6901 tagcgttgat cctctggttt attgatttag acattccttt tcagcaagtg atcaatgaaa
     6961 aatcatttgt tctagaatat acgatggcta ttcttaccac attgtccgca ctacttgttc
     7021 ttcctttaac ctgcgtgact acagcatatc tctttacaaa aaagaaagaa caactcaaga
     7081 tggagtaaaa gttacaactt aagtcctatg acatttttac tgaatcagtg cataatatgg
     7141 atgtaaggaa aagcttgcaa acaattttgc ttaaggaggc aatttccatg aaagaaagag
     7201 aacgtatttt agatttagtg aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat
     7261 tagaaagcat ggcaactgaa aaagacgaaa agcaaatcaa aaaagaagca gacaaggtaa
     7321 ctgctagtca taaagaaaaa gatcaggcaa gtcaattgat cgataaactt gaaaacggcg
     7381 aagaagaaat ttctgagcca gttgatccaa aggaaagaga aagacaagat caagaaaact
     7441 tagaaaagat cttagatgaa ctagcaactg aagcgaataa aacttcggcg cgtctagatg
     7501 aagtaaatgc tcaattcgct gacaataagg cagcacgcaa tgagaaacag gaagcattga
     7561 tgcagctgaa tacaaaagaa gaattaggcg agttgacaga agaagaatta gcacagcgtc
     7621 agacgttaga agcagaaatc aaagaattag aagctagtgg cgatacactg ttagaagaac
     7681 aattgaaatt agaagcagaa ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag
     7741 aaacgtttac tgataagttt gaattgccag atgattggaa agatcaagca acagatacac
     7801 tgaatcaagt aggcgaaaaa atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga
     7861 aaacattcca aactgtttca gaaacagtga atgacaacat ggaatggaaa gatgtcagct
     7921 tacgtgtccc tgggattgcg acaacgaaat ttgagcatga attttactat gaagctcctg
     7981 cagcaagtat ccttgatatc aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca
     8041 gcaacgatgt gaaagttgaa gctaagatca aactttacgg aaaaatgggt gctgaaccat
     8101 ttgaagcatt ctctgaacgg agccagattg aggttaatga agatcatatc tccttccaaa
     8161 ttccaaataa acgtgtgcgt gcagaccttg ttttctactt acctaaacga gtatacgacc
     8221 acgcagctat taaacttcta aatggcaaca tcatgattga aacattggaa gctaaagata
     8281 tttatacaaa atctacgaat ggtaacatca tcgtggacca attaactgca acgatgctgg
     8341 aagtagaagg tgtaaacggc aatatcgata ttcgaaacgg gaacatctta gattctatta
     8401 tcgaaacagt aaatggtaca gtgacatttg gtgcgacagc agaaaacctt tctgtgtctt
     8461 tggtaaatgg tgatgtacgt cttacgatta aagaagataa tttgaaaaaa gtagaagcaa
     8521 gttcagtgaa cggaaatgtc aaagtcgctt taccagatgg catcggtttg gaaggacatg
     8581 ctaaaacaag tcttggaagt atcaacagcc gtctgtccaa ttacgaagta gttcgtgaga
     8641 aaaaagaacg gacaaaccaa atgttgcaat tccgtcgtgt ttcagatggt gaaatcgcgc
     8701 aagtgcaact ttcaacgaca acaggaagta tctatttaaa agatacagat aaataaagag
     8761 gttgtgaaag agatgttcag ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc
     8821 tattttacaa aattgtgcct tattgccgat agtctatctc ttgaacaccg tttaccaaga
     8881 gattatgaac ctgccaatta tcgccgagtc acaaggaata atttcagaaa aagctcgtca
     8941 cgttttatga atatggtaat ttgtgaccga gaattttgca gaaagacacc gattaagaag
     9001 aaggttgtca caacgatttt cttcatttaa aatagtagaa cagaggtgag acatgatgaa
     9061 aaaaaagttg acaaaatcta ataaaaatgt tgttttgaca gggactttag caggaatagg
     9121 agaatacatc gggattgatc cgactgttat ccgtgtgctt tatgtattcc tgagcttagt
     9181 tgcttttgga agtccaatta ttttatacat tttattagct ttgattatcc catctgcgcg
     9241 ttctgcacga acaaactatg gacataacaa tgcatattat gaaaaaaaca attatcgtga
     9301 acaaactcgt gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg
     9361 gagtgacttt taatgtcgta ctttcaacgt attttagtta atacgttaac attcatatca
     9421 ttggctgttc tttttcctgc gatggtccat gtgagcagta tttggacggc cgttcttgcc
     9481 agcttcgtat tgtctgtgtt gaatatgttt gtcaaaccga ttttgacgat actgtccctg
     9541 ccctttacat tagtcacatt gggattgttt acgtttgtta tcaatgcagc gattcttaaa
     9601 atgacctcct tttttgtggg agcagaacat tttagttttt cttcattcgg tgcagcgttg
     9661 ttagtagcta tcttgatgtc gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa
     9721 taaaagaaag ctgtgccgaa ctatcggcgc agttttttta tttagaatca ggaatgcgtt
     9781 ttctattaca ttggtaatta aataaaaacg ttttttttaa ttggtagtac ttttggctag
     9841 acaatgataa aatgatagag aacactctaa tagaaatgag gtagcatgaa tgtcagaagt
     9901 tgtaaaagtt agccaattag tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa
     9961 aagcttagac cgtgtcatcg taactgggga catctcacgc ccaggactag agttgactgg
    10021 ttattttaat tattattccc ataatcgttt acagctattt ggcagtaaag aaatcagttt
    10081 tgcggaacgg atgatcccag aagagcgttt gatcatcatg agaagattat gtagtgaaga
    10141 tatgcccgca tttattattt caagaggatt accagctcca gaagaaatga tccaggcagc
    10201 aaatgaaaat gggatagctg tactgcgttc tcccatctca acttctcgtt tattaggaga
    10261 attatccagt tatttagatg gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt
    10321 ggatgtttac ggattaggtg tactgattca aggtgacagt gggataggaa aaagtgaaac
    10381 agcgttggag ttgatcaaac gcggccatcg attgatcgca gacgatcgag tagatgttta
    10441 tcagcaagat gaattaacgg tgatcggaga accgcctaaa atcctggaac atctgattga
    10501 aatccgagga atcgggatca ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg
    10561 ctctatgcaa gtccagttgg ctgtttatct ggaagcatgg gcaaaagata agaaatacga
    10621 ccgtctgggc tctgaagaca cgaccgttga aatcgcggaa gtgggcattc cacaaataaa
    10681 aatccctgtc aaaacaggac gaaacgttgc aatcatcatt gaagtagccg cgatgaattt
    10741 ccgcgcacgt acaatgggct ttgacgcaac gaaaacattt gaagaaagat taagtcgctt
    10801 gattgaagaa aactcaggca acgactagca aggagtgaca atatgttagc acaaatcaat
    10861 cgagttgctt ttgagcttgt agggattcct atctactggt atgcattgat catcgtatca
    10921 ggtatcatta tcgctatgtg gcttagcagc agagaggctg ttcgggtagg attaaaagca
    10981 gatgatgtga cagattttat gttgtgggga ttgcctatct ccattattgg cgctcgctta
    11041 tattatgttt tatttgatct tccccagtat atagctgatc caatccaaat attcaatatc
    11101 cgttcaggag gcttagctat ttatggcgga ctgattgcag gcgcgatcac gctgtatttt
    11161 tttactaaat atcattttat ttctttgtgg acttttttag atatcgctgc accaagtgta
    11221 ttattagcac aggcaatcgg tcgttgggga aatttcatga accacgaagc atatggacca
    11281 gacacgacgc gaagcttctt agaaagtctg catttaccgc aattcatcat tgataatatg
    11341 tatatcgaag ggatctatcg tcaaccaaca tttctttatg aatcactttg gagtttgatt
    11401 ggatttattt tgcttttgtt tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc
    11461 cttttatgtt atctaatgtg gtatagtttc gggcgtttct ttattgaagg attacgtaca
    11521 gacagtcttt actttttagg tgtgatccgt atttctcaat tattatctgc tgtgttattt
    11581 gtcggtacat tgattctatt tatttggcgc agaaaaaaag gaaatttacc ggattatgac
    11641 cgctcattag gaaaaaatca acaaattatc tgatttttag agcgttaaaa atgaaaaaaa
    11701 tttcaggagt ggtaaaatga attatagagt agcggtttta ggaccaggct cttggggtac
    11761 tgctttggca caagtcctcg ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc
    11821 ttcacaagta gaagaaatca acacccaaca cacgaatcaa cgttatttac cagatcatta
    11881 tctacccgat tccatccaag cattcaccaa tatggaagat gcagtaaaag acgcagatgc
    11941 ggtgctattc gtcattccaa cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa
    12001 attgaataca aagccagtga tcatccatgc aagtaaagga ttagaacagg gaacacataa
    12061 aaggatctct gacatcttgt tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt
    12121 tctttctgga ccaagccatg cagaagaagt agcaaaacat gacatcacta cgatcacagc
    12181 tgcaagtaca gatgcaaaag cagcaaaata tgtacaagag ctgttcatga ataattattt
    12241 tcggatctat accaatcctg atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat
    12301 tattgctatt ggtgctggcg ctattcacgg cttagggttt ggcgataatg caaaggcagc
    12361 aatcatgacg cgcggattag ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc
    12421 gctgaccttt atcggattaa gcggaatagg cgatctagtc gttacttgca caagtgttca
    12481 ttctaggaac tggcgcgcag gcaacttgtt aggacaaggg cagcccctag aagaggtgct
    12541 agccaatatg ggaatggtcg ttgaaggtgt gaatactacc caagcagcga tggaactagc
    12601 ccaaatgatg gaaatcgaaa tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca
    12661 aaaagaaatc aaaacagccg caaaagaaat catgttacgt gatggaaaaa aagaaaatga
    12721 attctcatta acaatttttt gaaggagcga atgtatatga aagttaaaaa agcagttatt
    12781 ccagcagcag gattaggaac aagattttta ccagcaacaa aagctatggc aaaggaaatg
    12841 ttgccaattg ttgataaacc aacaatccag ttcatcgttg aagaagcgtt gaaatcaggt
    12901 atcgaagaca tcttgatcgt tacaggaaaa gctaaacgac caatcgaaga tcactttgat
    12961 tcaaatattg aattagaaac aaacctaagt gaaaaaggaa agaccgaatt attgaaatta
    13021 gtagaagaaa cgacagatgt caacttgcat ttcatccgtc aatctcatcc aaagggttta
    13081 ggacatgccg ttcttcaagc aagagccttt attggaaatg aaccatttgt cgttatgcta
    13141 ggcgatgata taatggaaga cgaagtacca ttgacacagc aattaatcga tgattacgaa
    13201 cgtactcatg cttctacgat cgccgttatg caagttcctc atgatgaaac ttcaaaatac
    13261 ggaattatca atccaggaga agtattggaa gatgggttat acaatgtcaa aaactttgtt
    13321 gaaaaaccag atccaagcaa agcaccaagt gatttagcta ttattggacg ttacttattg
    13381 actccagaaa tttttgatgt attgtccgag caacaaccag gagccggaaa tgaaatccaa
    13441 ctaactgacg caatcgatac attgaataaa acacaacgcg tatttgcccg taaattcaca
    13501 ggtaaacgtt acgatgtcgg tgacaaattt ggttttatga aaacaagtat tgaatatggt
    13561 ctaacacatc cagaagtagg tgcaccttta cgtgagtaca tcctgtcttt aggtaaaaca
    13621 ttagaagcag aaggaagcaa gatcgatcat aatccaaaag gaataacaga aatcaaagaa
    13681 gaaaagaaaa aataaccctt ttttctaacg agcttcagct gctaattgaa taaaaagtag
    13741 ttgaagctcg tttttatttg tttaaaaaac acgaaaatgc tatccttata gaaaagagag
    13801 acaagaaggg agtttgatca tgtcaggagg agaaattgca ggattgatcg cagcaatcgc
    13861 ctttgcgata cttgtcgttt ttatcgttcg tgtgttgttg caagtttcta aaactttaga
    13921 aaaagtagat cagactgtag cagaagcaaa tacgacgatc gaaatcgtca caaaagacgt
    13981 agatcttttg tctagacaag tagaaggctt actggtcaaa agtaatgaac tgctcttgga
    14041 tatcaatgga aaagtcgcaa cgatcgatcc attgttcaca gcagttgctg atttaagtga
    14101 aagtgtatca gaacttaatc attctagtaa aaacatcgcg acgaaagtcg gcggaatcgg
    14161 taaaacgaca gctaaagcga cggttgccgg aaaagtcggt gggacagcga tgaaattttt
    14221 taaaaacaaa aaacatactg aaacgacagg aggaaaataa aaatggctaa aaaaggtgga
    14281 tttttattag gtgcagtagt cggaggcaca gccgcagcaa tcgccgcgtt attgttagca
    14341 ccaaaatctg gtaaagaact tcgggatgaa ttagctgccc aagcggatga tttgatggat
    14401 atggcttcag attacacaga tatggcgaaa gaaaaatcga ctgagttaac aggtattgca
    14461 aaagaaaaag cgcaagatct ttctcaacaa gcaggtgatc tagcaggtag tgtcaaacaa
    14521 aaagtaagtg aatcagcaga tgaagcagga agtgaatctg acgatatctt gttaaatctt
    14581 aaagaacaat cagctgactt atctaatcgt ttcaaaaaaa cggcagaaga tatgaaaaaa
    14641 cctgcagatg aattaggaag tattgctaaa gatacttcag aagatatcat gattgatgta
    14701 aaagacgctg ctggtaaagc aggagatact gtcaatgaag gaatgtcaga agcaaaacca
    14761 gttgcagatg actcaaaaga aatgatgaaa atggcaaaag aagatgcgaa agaagctaaa
    14821 aaagaaataa aagatacaat gacagattca tctacaaatc actaaaatca caaatttata
    14881 gaa
//
LOCUS       DAPCMU010000064        14815 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPCMU010000064 DAPCMU010000000
VERSION     DAPCMU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.066"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(296..1024)
                     /locus_tag="RD744_001591"
     CDS             complement(296..1024)
                     /locus_tag="RD744_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDU0557588.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="RD744_001592"
     CDS             complement(1002..2576)
                     /locus_tag="RD744_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDU0557589.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="RD744_001593"
     CDS             2805..3743
                     /locus_tag="RD744_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557590.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="RD744_001594"
     CDS             3755..4666
                     /locus_tag="RD744_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0557591.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="RD744_001595"
     CDS             4690..6162
                     /locus_tag="RD744_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDU0557592.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="RD744_001596"
     CDS             6213..6824
                     /locus_tag="RD744_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0557593.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="RD744_001597"
     CDS             6854..8767
                     /locus_tag="RD744_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDU0557594.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="RD744_001598"
     CDS             8895..11876
                     /locus_tag="RD744_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDU0557595.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="RD744_001599"
     CDS             11873..13534
                     /locus_tag="RD744_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDU0557596.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="RD744_001600"
     CDS             13574..14506
                     /locus_tag="RD744_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDU0557597.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAPCMU010000065        14655 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPCMU010000065 DAPCMU010000000
VERSION     DAPCMU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.067"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..626)
                     /locus_tag="RD744_001601"
     CDS             complement(<1..626)
                     /locus_tag="RD744_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0557598.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="RD744_001602"
     CDS             899..3370
                     /locus_tag="RD744_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0557599.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="RD744_001603"
     CDS             complement(3473..5209)
                     /locus_tag="RD744_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDU0557600.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="RD744_001604"
     CDS             complement(5206..5910)
                     /locus_tag="RD744_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557601.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="RD744_001605"
     CDS             6041..6544
                     /locus_tag="RD744_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDU0557602.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="RD744_001606"
     CDS             6504..6842
                     /locus_tag="RD744_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDU0557603.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="RD744_001607"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="RD744_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDU0557604.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="RD744_001608"
     CDS             8393..8971
                     /locus_tag="RD744_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDU0557605.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="RD744_001609"
     CDS             8975..9496
                     /locus_tag="RD744_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDU0557606.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="RD744_001610"
     CDS             complement(9575..10129)
                     /locus_tag="RD744_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDU0557607.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="RD744_001611"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="RD744_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDU0557608.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="RD744_001612"
     CDS             10669..10920
                     /locus_tag="RD744_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDU0557609.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="RD744_001613"
     CDS             11045..11836
                     /locus_tag="RD744_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557610.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="RD744_001614"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="RD744_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDU0557611.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="RD744_001615"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="RD744_001615"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDU0557612.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="RD744_001616"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="RD744_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDU0557613.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..14654)
                     /locus_tag="RD744_001617"
     CDS             complement(13893..14654)
                     /locus_tag="RD744_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0557614.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDY
                     KRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         4783 a         2675 c         2975 g         4222 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcata
//
LOCUS       DAPCMU010000066        14393 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPCMU010000066 DAPCMU010000000
VERSION     DAPCMU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.068"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="RD744_001618"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="RD744_001618"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDU0557615.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="RD744_001619"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="RD744_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDU0557616.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="RD744_001620"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="RD744_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDU0557617.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="RD744_001621"
     CDS             2857..3732
                     /locus_tag="RD744_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDU0557618.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="RD744_001622"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="RD744_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDU0557619.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="RD744_001623"
     CDS             4629..5063
                     /locus_tag="RD744_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDU0557620.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="RD744_001624"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="RD744_001624"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDU0557621.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="RD744_001625"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="RD744_001625"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDU0557622.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="RD744_001626"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="RD744_001626"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDU0557623.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="RD744_001627"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="RD744_001627"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDU0557624.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="RD744_001628"
     CDS             13334..14260
                     /locus_tag="RD744_001628"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDU0557625.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         5050 a         2494 c         3124 g         3725 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt tta
//
LOCUS       DAPCMU010000067        14381 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPCMU010000067 DAPCMU010000000
VERSION     DAPCMU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.069"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(27..923)
                     /locus_tag="RD744_001629"
     CDS             complement(27..923)
                     /locus_tag="RD744_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0557626.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="RD744_001630"
     CDS             complement(916..1596)
                     /locus_tag="RD744_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557627.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="RD744_001631"
     CDS             complement(1597..2379)
                     /locus_tag="RD744_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557628.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="RD744_001632"
     CDS             complement(2372..3289)
                     /locus_tag="RD744_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0557629.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3776)
                     /locus_tag="RD744_001633"
     CDS             complement(3453..3776)
                     /locus_tag="RD744_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557630.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4564..7791
                     /gene="fss3"
                     /locus_tag="RD744_001634"
     CDS             4564..7791
                     /gene="fss3"
                     /locus_tag="RD744_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDU0557631.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7904..8218
                     /locus_tag="RD744_001635"
     CDS             7904..8218
                     /locus_tag="RD744_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0557632.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8231..8605
                     /locus_tag="RD744_001636"
     CDS             8231..8605
                     /locus_tag="RD744_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0557633.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8615..8950
                     /locus_tag="RD744_001637"
     CDS             8615..8950
                     /locus_tag="RD744_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDU0557634.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9033..10373
                     /locus_tag="RD744_001638"
     CDS             9033..10373
                     /locus_tag="RD744_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDU0557635.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10451..11125
                     /locus_tag="RD744_001639"
     CDS             10451..11125
                     /locus_tag="RD744_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDU0557636.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11118..11282
                     /locus_tag="RD744_001640"
     CDS             11118..11282
                     /locus_tag="RD744_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557637.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11358..12500
                     /gene="dcm"
                     /locus_tag="RD744_001641"
                     /pseudo
     CDS             11358..12500
                     /gene="dcm"
                     /locus_tag="RD744_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12490..12780
                     /locus_tag="RD744_001642"
     CDS             12490..12780
                     /locus_tag="RD744_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557638.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13039..14223
                     /locus_tag="RD744_001643"
     CDS             13039..14223
                     /locus_tag="RD744_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0557639.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14220..14357
                     /locus_tag="RD744_001644"
     CDS             14220..14357
                     /locus_tag="RD744_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDU0557640.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4596 a         2632 c         3019 g         4134 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaaacct
     4321 tgatttataa aggttttccc gtaaatatat tttattatat ttgattaaat ttgaaatgtt
     4381 tgtgttctaa aatgtgttct aatttaaagg cagtatattt cgtattttag attaaatttt
     4441 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
     4501 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
     4561 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
     4621 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
     4681 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
     4741 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
     4801 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
     4861 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
     4921 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
     4981 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
     5041 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
     5101 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
     5161 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
     5221 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
     5281 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
     5341 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
     5401 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     5461 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     5521 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     5581 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     5641 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     5701 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     5761 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     5821 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     5881 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     5941 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     6001 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     6061 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     6121 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     6181 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     6241 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     6301 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     6361 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     6421 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     6481 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     6541 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     6601 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     6661 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     6721 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     6781 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     6841 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     6901 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     6961 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     7021 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     7081 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     7141 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     7201 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     7261 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     7321 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     7381 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     7441 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     7501 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     7561 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     7621 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     7681 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     7741 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     7801 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     7861 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     7921 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     7981 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     8041 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     8101 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     8161 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     8221 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     8281 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     8341 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     8401 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     8461 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     8521 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     8581 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     8641 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     8701 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     8761 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     8821 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     8881 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     8941 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     9001 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     9061 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     9121 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     9181 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     9241 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     9301 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     9361 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     9421 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     9481 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     9541 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     9601 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     9661 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     9721 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     9781 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     9841 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     9901 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     9961 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
    10021 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
    10081 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
    10141 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
    10201 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
    10261 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
    10321 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
    10381 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
    10441 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
    10501 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
    10561 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
    10621 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
    10681 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
    10741 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
    10801 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
    10861 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
    10921 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
    10981 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
    11041 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
    11101 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
    11161 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
    11221 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
    11281 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
    11341 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
    11401 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
    11461 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
    11521 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
    11581 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
    11641 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
    11701 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
    11761 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
    11821 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
    11881 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
    11941 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
    12001 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
    12061 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
    12121 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
    12181 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
    12241 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
    12301 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
    12361 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
    12421 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
    12481 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
    12541 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
    12601 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
    12661 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
    12721 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
    12781 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
    12841 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
    12901 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
    12961 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
    13021 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
    13081 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
    13141 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
    13201 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
    13261 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
    13321 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
    13381 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
    13441 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
    13501 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
    13561 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
    13621 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
    13681 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
    13741 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
    13801 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
    13861 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
    13921 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
    13981 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
    14041 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
    14101 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
    14161 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
    14221 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
    14281 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
    14341 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt t
//
LOCUS       DAPCMU010000068        14238 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPCMU010000068 DAPCMU010000000
VERSION     DAPCMU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.070"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..301
                     /locus_tag="RD744_001645"
     CDS             <1..301
                     /locus_tag="RD744_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDU0557641.1"
                     /translation="KKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFN
                     VSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            501..815
                     /locus_tag="RD744_001646"
     CDS             501..815
                     /locus_tag="RD744_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0557642.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            836..1213
                     /locus_tag="RD744_001647"
     CDS             836..1213
                     /locus_tag="RD744_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0557643.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1223..1996
                     /locus_tag="RD744_001648"
     CDS             1223..1996
                     /locus_tag="RD744_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557644.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2018..3421
                     /locus_tag="RD744_001649"
     CDS             2018..3421
                     /locus_tag="RD744_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDU0557645.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3603..4787
                     /locus_tag="RD744_001650"
     CDS             3603..4787
                     /locus_tag="RD744_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0557646.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4784..5074
                     /locus_tag="RD744_001651"
     CDS             4784..5074
                     /locus_tag="RD744_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557647.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5071..5292
                     /locus_tag="RD744_001652"
     CDS             5071..5292
                     /locus_tag="RD744_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557648.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5334..6113
                     /locus_tag="RD744_001653"
     CDS             5334..6113
                     /locus_tag="RD744_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDU0557649.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6180..6674
                     /locus_tag="RD744_001654"
     CDS             6180..6674
                     /locus_tag="RD744_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDU0557650.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6730..7368
                     /locus_tag="RD744_001655"
     CDS             6730..7368
                     /locus_tag="RD744_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557651.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7440..7835
                     /locus_tag="RD744_001656"
     CDS             7440..7835
                     /locus_tag="RD744_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0557652.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7819..10272
                     /locus_tag="RD744_001657"
     CDS             7819..10272
                     /locus_tag="RD744_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0557653.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10269..12296
                     /locus_tag="RD744_001658"
     CDS             10269..12296
                     /locus_tag="RD744_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557654.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12293..13315
                     /locus_tag="RD744_001659"
     CDS             12293..13315
                     /locus_tag="RD744_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDU0557655.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13332..>14238
                     /locus_tag="RD744_001660"
     CDS             13332..>14238
                     /locus_tag="RD744_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0557656.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQ"
BASE COUNT         4825 a         2408 c         2836 g         4169 t
ORIGIN      
        1 taagaagatg tttaaatctc gagatggtat cacacttact acagcacata aagttaaagg
       61 aaatgagtat gatactgtta tcgctattaa tcttctaaac gggaaaattc ctcattggaa
      121 tgaaattttt aatgtttctg ataaaggaat tagttcagcg agaaaactac tatacgtaat
      181 ttgttcaaga gcaaaaaata atttatttct tttctcagag gtgggttatt ttacgagtaa
      241 aggagacttg ctttatccca ctcaattatt agataaaata gaatttgatt ataatattta
      301 aaatacctag gtagtcgttg ttttaaatat agtaggaatc ggacgctcag tacggagcgt
      361 ctattttttt acccaatttt gaaaggaagt gaacttatga aaataaaaaa tcaagaatca
      421 aaaggtcgct ctcccccctt taaaaccatc acacaaataa gattcattca aacataaaaa
      481 cagaaaggat aggtaaaaat atggaactta aatatgtcat tcccaatatg gaaaaaacat
      541 ttggacaatt agaatatgct ggtgaaggga acattgaaca acgtcgaata aatggcagac
      601 caaccattct ctctcgtagt tacaatttat actcaacgat tcaacgagca gatgatattg
      661 ttgtcattct tccctccgaa gctggcgaga aacattttga atccgacgaa aaagtcaaac
      721 tcatcaaccc acatattacc gcagaaggtt ataaaattgg caatcgagga tttaccaatt
      781 acattatgca cgcagacgat atggtgaaag cataagaaag gaaaggtgaa aaaatatgag
      841 attagcagaa ggtattgtga ttgaaaaaga ccagacattt ggcacattga aattttccgc
      901 cttgcgtcgt gaggttcgta tccagaatga agatggtacg gtttccgaag aaattaaaga
      961 acgtacctat gacttaaaat caaaaggaca aggacgcatg atacaagtca gtatccccgc
     1021 ttctgttcct ttgaaagaat ttgactacaa cgcagaagtg gaattaatca atcctgtggc
     1081 agataccgta gcgacggcta cttttcgagg ggcagacgtt gattggtacg tgaaagcaga
     1141 cgatcttgta ttaaaaggac agacaaaacc tcatcagaaa tataaaaaag aagcgtccga
     1201 aagtgaaaaa taggtgttgc ttatgacaaa cacaccattg attttgaaag aaattccgaa
     1261 ggatgaagcc atttctttta ttcggcaata ccattattca aagatactcc ctagattgtg
     1321 taaatacttt ctagggattt tttcagaaga aaaattatta ggcgttgtgg aattaggctg
     1381 gggtacgcaa cccctacaaa ccatacgcaa actctttccc gacagttccc ttcaaaccac
     1441 agattattta gaaattggaa agatgtgttt tcttccagaa atgaatcaaa ccaattattt
     1501 tggcagtcaa gctctttcag ctctaattaa gtggttgaaa gaacatacgg actgtcattt
     1561 tttatataca ctggcagatg gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa
     1621 cttcttttac tgcgggtatt tcaagacaag tgtctatcgt gataagcagt catgggaaaa
     1681 gatacaccct cgaagtgctc gccttttatt agaggaaaat gctcgctttg aacaggtaga
     1741 gaaaaagcat tggctttccc aggcattttg cgaatacaaa gggattgaga aaatcaatgg
     1801 acgcatgttt cgctatctct atccgttgac aaaagaagcg aaaaaactgt taggacacac
     1861 tctctatcga cgtcattatt atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc
     1921 ttatcagaaa tatgaagcca tttctcaacc aacttttgat aaacaggctc gtatctacaa
     1981 tacacaatta ttttgatagg tggaaaggag gatttttatg ccattgcttc atgacagagg
     2041 agagcgaatc aaagccaacg ataagcactt ggtctatcat tttgtactaa tttcattgct
     2101 ggtagttttt ctagtcgtcc tactcccttt tcattggaga gcgttacaat cggtgaactg
     2161 gcaagcagtt cagttagata catttttcta tcaacttcat acgccatact tttggagcag
     2221 tattctcact gctctttttg tatgcggact tatcagctgg ctttattatc actatcgtat
     2281 agattctata aaacagttag agcaccgtca aaagttagct cgtatgattt tagaaaatca
     2341 ttggtatgaa gccgaagaaa ctcaatcaga gggattcttt aaagatttac ccagcaagaa
     2401 aaccaaacaa cgaatccgcc attttcctaa aatatattat cgaatgaaag atggactaat
     2461 ccatattcat gtagaaatca ccttagggaa atatcaagat cagcttttgc acctagaaaa
     2521 caaactagaa acaggattgt attgtgaatt agtggaaaaa acgttgaaag attcttatgt
     2581 agagtatgtc ctactttatg atactattgg caatcgaatt ggcattgaag acgtggtagc
     2641 tcaagatgga cgtgtcaaat tgatggaatc ggtctattgg gagttcgatt ctctccctca
     2701 catgttaatt gctgggggca ctggtagtgg aaaatcctat tttattctca cattgattga
     2761 atctctcctg catacaaatg caaagcttta tattcttgac cctaaaaatg ctgatttggc
     2821 agacttaggg gcggtcatga ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat
     2881 caatcaattt tacgaagata tgattgatcg cagtgaaacc atgaaacaac accctaacta
     2941 taaaacagga gaaaattatg cttatcttgg tttgtctgcc aattttctta tttttgatga
     3001 atatgtggca atgatggata tgctaggtcg tgaaagtacc accgttattc ataaactcaa
     3061 acaaattgta atgttaggac gacaagcagg cttcttttta attcttgcct gtcaacgtcc
     3121 agacgcaaaa tattttagtg atgggattcg ggacaacttt aacttccgtg tggctttggg
     3181 tcgtatgtct gaaatgggct ttggtatgat gttcggaagc gatacacaaa aacaattttt
     3241 cttaaaaccc atcaagggaa gaggttacgt ggatacagga aaaagtgtga ttagtgagtt
     3301 ttatacaccc cttgtaccaa aaaggtacga tttcttagga gaaataggaa aagtcattca
     3361 aaaaaagcaa tctgagcctg taccgcacga agtgaaaggt acaggttcag acttgctttg
     3421 attggtgtgg cgttagccac gccaatcctt tagccccctc tatctaacag gggggcacaa
     3481 atacaactga aaacaggcaa ttctcatcaa ccaatgttga tggtacaaag gattgcggaa
     3541 aatatgaaag gtgtaaactc aagggtcata gtttacatct tttttagatt ggaggtttgc
     3601 gaatggaaaa cgaaaaaatt tggattaagg aattgaaaga aaagcgactg gcttatggcg
     3661 tttctcaaaa taaactggcg gttgcttctc atattaccag accttaccta tcggatattg
     3721 aaacaggcaa ggcagtccct actcaacagg tgaaagaaga tttactgaac gctttggaac
     3781 gcttcaatcc cgataaccca ttggagatgt tattcgacta tgtacggatt cgattcccta
     3841 ccaatgatgt ggctcaaatt atcggtgagg ttttacgttt gaatatggat tatatgctac
     3901 acgaagactt tggctattat tcttatcccg aacactatcg tttcggtgat attgttgtcc
     3961 tagtctctca tgatgtaagt aaaggtgttt tattagaact gaaaggaaaa ggctgtcggc
     4021 aatttgaaaa ctttctattg gctcaacatc ggagctggta tgatttcttt caagattgca
     4081 tggaacataa aggtattttc aaacgcttgg atttagctat caacgataaa acagggattt
     4141 tgaatattcc agatttggca aagaaatgta aacaggaaga atgcatttcc gtctttcgaa
     4201 gctttaaaaa ttatcgcagt ggtgaattgg ttcaccgtga tgaaaaacca tacatgggaa
     4261 acacacttta tattggctca ttaaaaagtg aagtctattt ctgtatatat gagaaagatt
     4321 atgagcaata cgtgaaaaat gatgttccct tagaagacgc agaagtaaag aatcggtttg
     4381 aaattcggtt aaaaaatgac cgagccaccc aagccatgaa agatttattg gcacatcaac
     4441 aggcggaaaa aacagccttt gaaattatca atcgctatat ccgctttgca gataaagacg
     4501 acactaaacg tcggagtgat tggaaaacta atgaacgttg ggaatggttt atcggtaaaa
     4561 atcgtggagc attacgccta accacacagc cagagcctta ttcttttgaa cgaacattga
     4621 actggttaca tcatcaagtc gcccctacat taaaaatcgc aagcatttta gatgttctca
     4681 acggtacaac cattatctcc accatgattc aggaagccaa actgacagaa aaacatgaga
     4741 aattaattga acaacaacat ctagccatgg aggatttgat tacatgagcc ttttaacaaa
     4801 agaattaaag aagttagggt ttcagtgtgg cattgagttt caagcgtata tacaaaatac
     4861 agggaaatat acgtctctca ttattgaggg aaaacggcaa gctggtgata ccatctatac
     4921 ctatgatttt tacaaggtga gattttataa taattatacc aatcgagtaa ccgtctatgg
     4981 agagcattta accccttttc aattattaag gcgtgtaaaa agctatatct attatcgaga
     5041 aaaatatctt aaggaaagga ggacaatcac atgaattttg ggcagaattt gtacaactgg
     5101 ttcttggata atgcacagtc attggtattg ttagcgattg ttgtcattgg actgttttta
     5161 ggttttaaac gagaattttc aaaactcatt ggcttcttga ttattgctct aatcgccgtc
     5221 ggacttgtgt tcaatgcagc tggcgtgaaa gatgtgttat taaacttatt taaccgaatc
     5281 attggtgctt aacagatatt tttcatgata aaataggaag tatatatttt gttatgaaag
     5341 gatttatgaa tatgtttaca aatgatcaat ttaaatctct tttaattaaa acattagaaa
     5401 gaaagagctc ttcaaattat tatgaaggag gtaacgagat tgtttctaaa atgaaaatat
     5461 cagaggtaac tttagatgag actgacgatt ttacttatta taaaggatat aaaaagggct
     5521 ggaatacgct ttgcacatat cttaaaataa gagttccttt tgatgattta gacttttttg
     5581 aaagtcataa agatttgatt acccagacag caagctccat atatgataaa caaggtgata
     5641 atgtattagt ggatactatt ttagtaccac ttccagagaa ttacgaagta attaacttta
     5701 gtcagttgaa aattagtgat gtggtttcac aagctattga agacgctgag tcttttatga
     5761 gtaatggtga atatcaacgt gcctttgaca gagttcacac tgcttttcat ggatatttaa
     5821 ttgaaatact aaaaaaatat gaaattacag ttcctagaga cgaaaatcta tctaaattat
     5881 atagtaggat acaacaatta attgaaaaag aaattcaacc tactgaactt gctgatatag
     5941 tgaagacaac gataaggtca tcaaatggaa tgattagctc tcttaatgaa gcaagaaata
     6001 ggcattcact tgcacaccct aatacaaata ttattggaaa gagagaagca aaactaataa
     6061 taggtatttc aagtacagtt acagattata ttagcggata ccttgataag tagcgtcatt
     6121 aactgattgc tacttttttt atgactattt ttataaaaag gatgtgataa gaattggaaa
     6181 tgaaagtcta tgtggcaaat ttaggacggt ataatgaagg tgaattagtc ggagcatggt
     6241 ttactccacc aattgatgag gaagaaatgg cagaaagaat cggtttaaat gaggactacg
     6301 aagaatatgc aatacatgat tttgagcttc cttttgatgt ggacgaatac acgccgatta
     6361 gtgaaatcaa ccgtctatgt gaagccattc aagaaatcga aggtacacct atttataatg
     6421 aactcaaaga aattcaaggc atgtggttta gtagcttgga agaattactg gaaaacaaag
     6481 aggatattca ttgttatagt gattgtgatt cgatggaaga tgtcgcccgt tattacgtgg
     6541 aagaaacagg acagcttggt gaagtccctt ccaatttaca aaattatatt gactaccaat
     6601 ctttgggacg tgatatggaa atagagggta attacctagt tacctctcat ggggtatttg
     6661 aatattgtca gtaagtttaa caatccgtgt tataattaaa ttaataaata gcacggagag
     6721 gtataaatta tggtaaatgt atttgaaaat cttaaactat taaaactaga catgaaagat
     6781 aaaggctggg tgatagattc cttttacttt cgttacaaac agcaaaatta tattgtactt
     6841 gttaagctat ttgaaaaaga ggaaaaagtt ccagaatatg ctctgttaaa actagaattt
     6901 ttaaaggaaa acgatttttc agatatgcta gctgtctatg ctaattcggt aaaattatat
     6961 acagacacga aaacaataag agaatatttt ggtatcgaat acagtagtaa cttgggagat
     7021 gttttatttc aatttagcca aactcttgct cgatttattc cgacagaagt tagtgagaag
     7081 aaaaatgaag accaaaaaga agccatgtgt ttctcattaa gtcaaagtga ttcagaagat
     7141 cccagaaaaa aatattgttt ttcagttaga agaaatcctt taaagggaaa tggtgagctg
     7201 ggaaaacgaa gtccttttaa tgacaataaa acgagattgt atagacccag cttatatgaa
     7261 cgattagggt cagataccaa tttaagtttc agatattcaa tgaatccaga tgatgaagaa
     7321 actgatgaag gtattattgc aaaatggaca aaaaataaaa ttgaatagtt aaagcattga
     7381 aagagcaatc acctattttg attgttcttt ttctattttt acggaaagga cgtgaagaca
     7441 tgaaaaaaat aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcgatta
     7501 atgatttaca actccctttc ccaattacct ttacgcaaat gacatggttt atcgtttctt
     7561 tgtttgtcgt catactcttt ggcagtttac caccactttc tctgattgac ggagcactac
     7621 tcaaatactt agggattccc attgttctca ctgggttcat gtgccaaaag acctttgacg
     7681 gaaagaaacc ctatggattt ctaaagtcta tcattaccta tgctctacgc cctaaagtaa
     7741 cctacgctgg caaacaaata cgattaaaaa aagaaacaat gaatgaatcg attacgattg
     7801 taaggagtga acaactagat ggcatatccg attaaatata tggaagataa tctcgtattt
     7861 aatcatgatg gcgaagtatt cgcttattat gaattaattc cctataatta cagtttttta
     7921 agttccgatg aaaaaattca agttcatgat aatttctgtc aattgattgc ccaaaatcgt
     7981 gatggcaaga ttcatgctct gcaactagca accgaatcaa gtattcgagc cacacaggaa
     8041 caatcgaaaa aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa
     8101 cagaccgaag cgttaatttc tatgattggt gacaatcaag tagactatcg cttttttatt
     8161 ggttttaaac tctacctcaa tgaacaggaa gtatccgtaa aaaatgttgg gaaagaaatt
     8221 caaaaagcat ttgcggaatt ctttcatggt gtgaatcaca aattaatggg cgattttgtt
     8281 tctatgccta tggcggaaat cgaacgcttt tctaagatgg aacagttgtt agaaagtaaa
     8341 ttatccagac gctttcagat tcgtaaattg gagaaagatg attttggtta tattattgaa
     8401 catctttacg ggcaaacagg tgtccctttt gatgactacg actaccattt accgaaacga
     8461 tacgaagata aaaaagtgtt ggtaaagaaa tacgatattc tcaaacccac tcgctgttta
     8521 atcgaagaaa atcaacgcta tttacggatt gaacaggaag ataccactac ttatgtggcg
     8581 tattttacaa ttaatagtat tgttggtgaa ttggatttcc ctaatagtga aattttttat
     8641 taccagcaac agcagtttac tttccccgtt gataccagta tgaatgttga aattgtcgcc
     8701 aataaaaaag ccttatctac cgttcgcaat aaaaagaaag aactgaaaga tttagatagc
     8761 cacgcatggg aaagcgacaa cgaaaccagt actaatgtgg tggacgcttt agaatctgtc
     8821 gatgaattag aaagtaattt agaccaaacc aaagaatcta tgtacaaact aagctatgtg
     8881 gtacgggtat cagcaaagaa tttggacgag ctgaaacaac gttgtgatga agtaaaagat
     8941 ttttatgatg atctgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat
     9001 aatgaattta ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg
     9061 gattttttgg ctggtcttgg tttcggtgct acacaaatga ttggtgaaaa ttctggtatt
     9121 tacgtcggtt acaatctcga tacaggcaga aatatttatc tccaacctag ccttgcaagt
     9181 caaggaatta aaggctctgt aacaaatgca ttagcgtccg cttttattgg ttcattaggt
     9241 ggcggaaaat cattctctaa caatatgctg gtctattatt cagtactctt tggcggacag
     9301 gcaaccattg ttgacccaaa agctgaacgt ggtgaatgga aagaaacctt acctgatatt
     9361 gcccatgaaa tcaatattgt taatctcacg agtgagaacg acaataaagg gttacttgac
     9421 ccttatgtga ttttaaaact cccgaaagat tcagaaagcc ttgccattga tattctcacg
     9481 ttcttaacag gtatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagctatt
     9541 cggagtgtaa ctcaaagcaa acaacgaggg ttgttattgg tgattgatga attgcgaaaa
     9601 gaagatacgc ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat
     9661 tttgcccatc tgttattcag tgatggcagt gtcacccaat ccattagctt ggaaaaacaa
     9721 ctcaatatta ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa
     9781 tacactacca tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta
     9841 gactttatcc actctgaccg ttccattttt aaaattgttg accttgatga ggcttggagt
     9901 tttttacaag tggcacaagg aaaagctcta tctatgcgat tggttcgagc aggacgtgcc
     9961 atgaatgcag gggtctattt cgttactcaa aacgcggacg atttactaga tgaaaagatg
    10021 aagaataata ttggcttgaa atttgccttt cgttccactg acctagtgga aatcaaaaag
    10081 accttagaat tttttggtgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa
    10141 ttagaaaatg gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt
    10201 cacccgattt ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag
    10261 gtggaagaat gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt
    10321 gtgtgggcgt tattttactt ttattaggca cgctgggcaa tgcagtacaa gcgacaggct
    10381 tagttgatga aaccattgat acaagcaatg aatactccaa gtatggtttg aatcaatatc
    10441 agcttgatta ttatgtggac aatagctggg gctggcttcc atggaattgg agtgatggca
    10501 taggacaatc ggtgatgtat ggattgtatg ccattactaa ttttatttgg acaatttctc
    10561 tttatctatc gaacgccaca ggttatttgg tgaaagaagc ctatgcctta gatttcatta
    10621 gtcaaacatc ccaagcaatt ggcgaaaaca tgcagacgat tgcaggcatt acaccgaatg
    10681 gcttttccag ttcagggttt tatgtcggtt tcctccttct ttttattctc attgttggaa
    10741 tctatgttgc ctatacagga ttggttaagc gagaaacgac gaaagtgata cgagctgttt
    10801 taaactttgt cgtggtgttt attctatcgg cttcctttat tgcgtatgca cccgattata
    10861 ttgcgaaaat caatgatttt tccaaagatg tcagtaccgc cagtctgaat attgggacaa
    10921 aaattgttct tcctaacacg gatagtcaag ggaaagacag tgtggacatg atacgtgaca
    10981 gcttattctc tgttcaagta aagcaaccat ggctattgct tcaatatggc tccactgatg
    11041 tagaagcgtt aggggaagaa cgtgtggaaa gtttattgtc taccagtcca gatacaaaca
    11101 atggtgaaga tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt
    11161 taaccctacc gaagacgatt tccagattag gaacagtctt ctttttgttt gtattcaata
    11221 ttggtatctc gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat
    11281 ttattatttt cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg
    11341 atggcatggg caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta
    11401 ttaccttgat tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa
    11461 gttctccctt tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta
    11521 aactgggcga tttaatgagc ctattcactt tacaaagcaa cgactctcaa aacatgggca
    11581 gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca
    11641 gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa
    11701 agcagaatca aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga
    11761 aagaaacagc actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg
    11821 aaaagacagc ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcaa
    11881 aacaacagat acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga
    11941 atgtcactca taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca
    12001 aacaacagcg tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg
    12061 agctggataa agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata
    12121 accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta
    12181 aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc
    12241 aattcaatca gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct
    12301 aaaaataaca gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct
    12361 agtcattttc tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctattcc
    12421 acaaggcggt gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata
    12481 tggtaaggaa tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga
    12541 atcgggcggt acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa
    12601 cagtttgagt accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac
    12661 cagtgcagaa caacaaggag tggatataga ttctgttatc caatcttata attatggcgg
    12721 tggcttttta aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca
    12781 gttttctaaa gagaagtcgg gtggtcaaaa agcagactat cccaatccta tagccatacc
    12841 tgtgaatggt ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca
    12901 atacctaacc gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga
    12961 cgaagccttg aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc
    13021 ttttgattgt agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg
    13081 tgtcgcccaa gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg
    13141 cgaccttatc ttctttcatt caacctataa tgcaggcaca tatgtgactc atgtggcgat
    13201 atatttagag ggaaatcgct tttatcatgc aggcgaccca atcggttatg gcgacttaag
    13261 cagtcgttat tggcaagacc atctgattgg ggctagacgt gttattcata actaagaaag
    13321 gaatgaatca aatgattata aaaatcgagc gaaaagaaaa gaaggagaaa ccaaagaaat
    13381 ctaaaaaacc gagcaagcaa agaaaagttc cgatgattaa agttggcacg cataagaaat
    13441 taacctttgt tttatgggtt ttattgattg gtagtgtcgg atttggaatc tataaaaatt
    13501 ttactgccat tgacactcat actgtccgtg aaacagaaat cattaaacag cagattgtcg
    13561 ataccaacca agtagaaagt tttgtgaaat cctttgcgaa agactacttt tcgtggcaac
    13621 aatcccaaga agccattgat aaacgaaatg aaaaattgac ccattatttg acggaagaat
    13681 tgcaggtatt aaatgaagaa atgattcgta aggatattcc tactagttct agtgtgaacg
    13741 atatacaggt gtggcaggtt tctcaagtga atgagaacac ctttgaggta ctgttttctg
    13801 ttgaacaagt tattaccgaa gacaaagaca aggaaactat atcgtctagc tttcatgtgg
    13861 ttgtccatat agatgaatca gataatatgg ttattatcaa aaatccgaca atgagtaaga
    13921 aacctcaaaa atcggactac caacccaaac agctagaaag tgaccataca gtagatacag
    13981 aaacgatgga cgaaatcact agctttttag aaacgttctt ccagctctat cctaccgcca
    14041 cagaaaaaga attgacttac tatgtaagta atcatgtgtt acctatgatt aacaaggaat
    14101 atgttttcga ggaattggtg aatcctatct ttaccagaaa agataaccaa gtaatcgtga
    14161 atgtcgctgt gaaatattta gatcaagaaa caaaggcaac ccaaatctcg caatttgagt
    14221 tgatattaga aaaacaag
//
LOCUS       DAPCMU010000069        13829 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPCMU010000069 DAPCMU010000000
VERSION     DAPCMU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.071"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..902)
                     /locus_tag="RD744_001661"
     CDS             complement(<1..902)
                     /locus_tag="RD744_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDU0557657.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="RD744_001662"
     CDS             1185..2153
                     /locus_tag="RD744_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0557658.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="RD744_001663"
     CDS             2266..2544
                     /locus_tag="RD744_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDU0557659.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="RD744_001664"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="RD744_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDU0557660.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="RD744_001665"
     CDS             3001..3756
                     /locus_tag="RD744_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0557661.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="RD744_001666"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="RD744_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDU0557662.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="RD744_001667"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="RD744_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDU0557663.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="RD744_001668"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="RD744_001668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDU0557664.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="RD744_001669"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="RD744_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDU0557665.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="RD744_001670"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="RD744_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDU0557666.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="RD744_001671"
     CDS             8493..9035
                     /locus_tag="RD744_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDU0557667.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="RD744_001672"
     CDS             9058..10614
                     /locus_tag="RD744_001672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDU0557668.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="RD744_001673"
     CDS             10628..11530
                     /locus_tag="RD744_001673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDU0557669.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="RD744_001674"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="RD744_001674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDU0557670.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="RD744_001675"
     CDS             12973..13395
                     /locus_tag="RD744_001675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDU0557671.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4569 a         2472 c         3017 g         3771 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgggc tctttgtcaa taaggactga tgagttgtgc
    13741 aaaatcaaat ctgagtcaga atgaaccaca ttctggctca gattttggct ctttgtcaaa
    13801 taggactgat agagtgagat aagaacatc
//
LOCUS       DAPCMU010000070        13648 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPCMU010000070 DAPCMU010000000
VERSION     DAPCMU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.072"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            75..317
                     /gene="yaaA"
                     /locus_tag="RD744_001676"
     CDS             75..317
                     /gene="yaaA"
                     /locus_tag="RD744_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDU0557672.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            304..1428
                     /gene="recF"
                     /locus_tag="RD744_001677"
     CDS             304..1428
                     /gene="recF"
                     /locus_tag="RD744_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDU0557673.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1425..3371
                     /gene="gyrB"
                     /locus_tag="RD744_001678"
     CDS             1425..3371
                     /gene="gyrB"
                     /locus_tag="RD744_001678"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDU0557674.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3391..5862
                     /gene="gyrA"
                     /locus_tag="RD744_001679"
     CDS             3391..5862
                     /gene="gyrA"
                     /locus_tag="RD744_001679"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDU0557675.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6098..6397
                     /locus_tag="RD744_001680"
     CDS             6098..6397
                     /locus_tag="RD744_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDU0557676.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6447..6959
                     /gene="ssb"
                     /locus_tag="RD744_001681"
     CDS             6447..6959
                     /gene="ssb"
                     /locus_tag="RD744_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0557677.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6985..7221
                     /locus_tag="RD744_001682"
     CDS             6985..7221
                     /locus_tag="RD744_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDU0557678.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7364..9337
                     /locus_tag="RD744_001683"
     CDS             7364..9337
                     /locus_tag="RD744_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDU0557679.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9343..9795
                     /gene="rplI"
                     /locus_tag="RD744_001684"
     CDS             9343..9795
                     /gene="rplI"
                     /locus_tag="RD744_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDU0557680.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10069..11436
                     /gene="dnaB"
                     /locus_tag="RD744_001685"
     CDS             10069..11436
                     /gene="dnaB"
                     /locus_tag="RD744_001685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDU0557681.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12216..13508
                     /locus_tag="RD744_001686"
     CDS             12216..13508
                     /locus_tag="RD744_001686"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDU0557682.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4549 a         2467 c         3003 g         3629 t
ORIGIN      
        1 ggataaattt gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa
       61 atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc
      121 aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag
      181 aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg
      241 gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg
      301 ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc
      361 gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt
      421 ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa
      481 ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc
      541 actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc
      601 gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa
      661 gatctttctt tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta
      721 ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa
      781 agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat
      841 attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc
      901 gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag
      961 cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa
     1021 atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga
     1081 acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg
     1141 cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa
     1201 atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg
     1261 agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa
     1321 acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag
     1381 attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa
     1441 gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg
     1501 aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc
     1561 accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga
     1621 aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga
     1681 tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt
     1741 tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag
     1801 taggatcttc tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag
     1861 gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg
     1921 aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag
     1981 agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga
     2041 accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat
     2101 accattatga cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga
     2161 tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta
     2221 tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt
     2281 atgaaggcgg aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt
     2341 atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc
     2401 gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa
     2461 caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac
     2521 atttcatgaa attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat
     2581 tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag
     2641 gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg
     2701 aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc
     2761 gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag
     2821 caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta
     2881 cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga
     2941 ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt
     3001 acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg
     3061 taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga
     3121 aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac
     3181 ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga
     3241 tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg
     3301 gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact
     3361 tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat
     3421 gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt
     3481 gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta
     3541 tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc
     3601 gtcggagatg tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg
     3661 gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt
     3721 ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa
     3781 atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat
     3841 gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac
     3901 ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag
     3961 gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg
     4021 gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc
     4081 cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact
     4141 gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa
     4201 gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca
     4261 gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac
     4321 gccagcgcat ctgttatctt gaataactta tataaattga catccttgca aaattcattt
     4381 ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt
     4441 cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa
     4501 gaaaaagcag aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc
     4561 gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg
     4621 atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc
     4681 cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg
     4741 attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat
     4801 gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt
     4861 gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg
     4921 acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt
     4981 ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct
     5041 tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa
     5101 ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta
     5161 ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa
     5221 ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct
     5281 tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc
     5341 gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac
     5401 tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga
     5461 ggaatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa
     5521 gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca
     5581 gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca
     5641 ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt
     5701 gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta
     5761 cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa
     5821 gctgcagaac aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg
     5881 taagctagca agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag
     5941 ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga
     6001 gtgtataatc agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga
     6061 acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat
     6121 catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt
     6181 cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg
     6241 ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc
     6301 ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga
     6361 tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata
     6421 gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga
     6481 cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg
     6541 ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg
     6601 tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag
     6661 gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg
     6721 tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc
     6781 gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca
     6841 ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt
     6901 ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa
     6961 cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt
     7021 aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta
     7081 ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc
     7141 gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta
     7201 cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc
     7261 tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga
     7321 aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa
     7381 tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt
     7441 cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg
     7501 tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca
     7561 gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg
     7621 agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa
     7681 aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa
     7741 agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac
     7801 agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca
     7861 gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag atgccattga
     7921 taaattggat gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga
     7981 ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt
     8041 agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt
     8101 tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg
     8161 ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt
     8221 ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta
     8281 tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac
     8341 cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc
     8401 ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa
     8461 aaaagcttac atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga
     8521 agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga
     8581 gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat
     8641 ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg
     8701 ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc
     8761 agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt
     8821 tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac
     8881 aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt
     8941 agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc
     9001 tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta
     9061 taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc
     9121 agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg
     9181 cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc
     9241 agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat
     9301 caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta
     9361 caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca
     9421 caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag
     9481 ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa
     9541 aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg
     9601 gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa
     9661 caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta
     9721 ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat
     9781 gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga
     9841 aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca
     9901 aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa
     9961 cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc
    10021 tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg
    10081 tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt
    10141 tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat
    10201 cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg
    10261 attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt
    10321 ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac
    10381 tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga
    10441 atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa
    10501 cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa
    10561 gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca
    10621 ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa
    10681 ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct
    10741 caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca
    10801 gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt
    10861 acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct
    10921 cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa
    10981 tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag
    11041 ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa
    11101 ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt
    11161 ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca
    11221 attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa
    11281 ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc
    11341 gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat
    11401 aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca
    11461 atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta
    11521 atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa
    11581 taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga
    11641 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt
    11701 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg
    11761 agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
    11821 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa
    11881 tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca
    11941 acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa
    12001 aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt
    12061 acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat
    12121 aaaataaaaa taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg
    12181 gttcaggatt aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta
    12241 cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag
    12301 tgattgcacg gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga
    12361 cttataaact gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg
    12421 gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa
    12481 aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc
    12541 atatcaaatt ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga
    12601 ttaaaggaat cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag
    12661 acttattaga taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc
    12721 gtcaatttgt aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat
    12781 attatgaata cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg
    12841 atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata
    12901 tcgaccaggg aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa
    12961 tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata
    13021 cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa
    13081 tccgcgaagt aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt
    13141 ttgattctgt agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa
    13201 actcgatcga tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag
    13261 atggcgaact aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa
    13321 tctatgaaga attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac
    13381 tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct
    13441 caacattttc tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc
    13501 aaatctaagg ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg
    13561 tttatctata cgataataat aacttgaaca ctgtttattc agaataaggg actgtgacaa
    13621 aaaccgtgtc acagtccctt tttccgaa
//
LOCUS       DAPCMU010000071        13398 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPCMU010000071 DAPCMU010000000
VERSION     DAPCMU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.073"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(15..707)
                     /locus_tag="RD744_001687"
     CDS             complement(15..707)
                     /locus_tag="RD744_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDU0557683.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="RD744_001688"
     CDS             complement(1144..1713)
                     /locus_tag="RD744_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0557684.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="RD744_001689"
     CDS             complement(1787..2110)
                     /locus_tag="RD744_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557685.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="RD744_001690"
     CDS             complement(2264..3076)
                     /locus_tag="RD744_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557686.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="RD744_001691"
     CDS             3430..4278
                     /locus_tag="RD744_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDU0557687.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="RD744_001692"
     CDS             4423..5364
                     /locus_tag="RD744_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDU0557688.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="RD744_001693"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="RD744_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="RD744_001694"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="RD744_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDU0557689.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="RD744_001695"
     CDS             complement(9828..12014)
                     /locus_tag="RD744_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDU0557690.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="RD744_001696"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="RD744_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDU0557691.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="RD744_001697"
     CDS             complement(12236..12676)
                     /locus_tag="RD744_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDU0557692.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="RD744_001698"
     CDS             complement(12750..13289)
                     /locus_tag="RD744_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0557693.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3764 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaata
//
LOCUS       DAPCMU010000072        13212 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPCMU010000072 DAPCMU010000000
VERSION     DAPCMU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.075"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            147..1016
                     /locus_tag="RD744_001699"
     CDS             147..1016
                     /locus_tag="RD744_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDU0557694.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="RD744_001700"
     CDS             1225..2250
                     /locus_tag="RD744_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDU0557695.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="RD744_001701"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="RD744_001701"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDU0557696.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="RD744_001702"
     CDS             3333..5036
                     /locus_tag="RD744_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0557697.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="RD744_001703"
     CDS             5038..7071
                     /locus_tag="RD744_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDU0557698.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="RD744_001704"
     CDS             7082..7522
                     /locus_tag="RD744_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0557699.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="RD744_001705"
     CDS             complement(7722..8849)
                     /locus_tag="RD744_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557700.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="RD744_001706"
     CDS             complement(8852..9529)
                     /locus_tag="RD744_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557701.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="RD744_001707"
     CDS             9793..11559
                     /locus_tag="RD744_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDU0557702.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="RD744_001708"
     CDS             11574..12011
                     /locus_tag="RD744_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0557703.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="RD744_001709"
     CDS             12024..13175
                     /locus_tag="RD744_001709"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDU0557704.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAPCMU010000073        13175 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPCMU010000073 DAPCMU010000000
VERSION     DAPCMU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.076"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(6..335)
                     /locus_tag="RD744_001710"
     CDS             complement(6..335)
                     /locus_tag="RD744_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDU0557705.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="RD744_001711"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="RD744_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDU0557706.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="RD744_001712"
     CDS             1256..2269
                     /locus_tag="RD744_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0557707.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="RD744_001713"
     CDS             2269..3546
                     /locus_tag="RD744_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDU0557708.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="RD744_001714"
     CDS             3609..6311
                     /locus_tag="RD744_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0557709.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="RD744_001715"
     CDS             6463..6780
                     /locus_tag="RD744_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0557710.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="RD744_001716"
     CDS             6810..7130
                     /locus_tag="RD744_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0557711.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="RD744_001717"
     CDS             7238..8698
                     /locus_tag="RD744_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0557712.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="RD744_001718"
     CDS             8766..8987
                     /locus_tag="RD744_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557713.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="RD744_001719"
     CDS             9018..9200
                     /locus_tag="RD744_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDU0557714.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="RD744_001720"
     CDS             9200..9613
                     /locus_tag="RD744_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDU0557715.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="RD744_001721"
     CDS             9736..10917
                     /locus_tag="RD744_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0557716.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10988..11152)
                     /locus_tag="RD744_001722"
     CDS             complement(10988..11152)
                     /locus_tag="RD744_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557717.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11306..11379
                     /locus_tag="RD744_001723"
     tRNA            11306..11379
                     /locus_tag="RD744_001723"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11416..11616)
                     /locus_tag="RD744_001724"
     CDS             complement(11416..11616)
                     /locus_tag="RD744_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0557718.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(11921..13153)
                     /locus_tag="RD744_001725"
     CDS             complement(11921..13153)
                     /locus_tag="RD744_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDU0557719.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         4242 a         2297 c         2750 g         3886 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagag ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt acaaataacc cctgtatcct
    11401 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
    11461 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
    11521 atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga
    11581 aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg
    11641 atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg
    11701 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
    11761 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
    11821 aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc
    11881 tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc
    11941 ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc
    12001 ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat
    12061 cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat
    12121 tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg
    12181 aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca
    12241 gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa
    12301 aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa
    12361 tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg
    12421 ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag
    12481 gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc
    12541 gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag
    12601 ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt
    12661 tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga
    12721 cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat
    12781 tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg
    12841 cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt
    12901 tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga
    12961 tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg
    13021 tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt
    13081 tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa
    13141 aatcagataa cataataaaa attcctcctc accat
//
LOCUS       DAPCMU010000074        13150 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPCMU010000074 DAPCMU010000000
VERSION     DAPCMU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.077"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            114..1853
                     /gene="yfcC"
                     /locus_tag="RD744_001726"
     CDS             114..1853
                     /gene="yfcC"
                     /locus_tag="RD744_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDU0557720.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2010..3194
                     /gene="iadA"
                     /locus_tag="RD744_001727"
     CDS             2010..3194
                     /gene="iadA"
                     /locus_tag="RD744_001727"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDU0557721.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3305..3508
                     /locus_tag="RD744_001728"
     CDS             3305..3508
                     /locus_tag="RD744_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDU0557722.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3609..4271)
                     /locus_tag="RD744_001729"
     CDS             complement(3609..4271)
                     /locus_tag="RD744_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557723.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4284..5786)
                     /locus_tag="RD744_001730"
     CDS             complement(4284..5786)
                     /locus_tag="RD744_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557724.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6334..8013
                     /locus_tag="RD744_001731"
     CDS             6334..8013
                     /locus_tag="RD744_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDU0557725.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8140..8619
                     /locus_tag="RD744_001732"
     CDS             8140..8619
                     /locus_tag="RD744_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDU0557726.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8838..9518
                     /locus_tag="RD744_001733"
     CDS             8838..9518
                     /locus_tag="RD744_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDU0557727.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9526..10857
                     /locus_tag="RD744_001734"
     CDS             9526..10857
                     /locus_tag="RD744_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDU0557728.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10872..11834
                     /locus_tag="RD744_001735"
     CDS             10872..11834
                     /locus_tag="RD744_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDU0557729.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11945..12283
                     /locus_tag="RD744_001736"
     CDS             11945..12283
                     /locus_tag="RD744_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDU0557730.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12362..13069
                     /locus_tag="RD744_001737"
     CDS             12362..13069
                     /locus_tag="RD744_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDU0557731.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4177 a         2321 c         2884 g         3768 t
ORIGIN      
        1 agtgattttc taataaatta ttcatgtgga aatgtcattt ttactgtata attcatttga
       61 ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata aatttgaaga
      121 aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca ccccatacgt
      181 atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc gttccagcag
      241 gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca agtactcgta
      301 ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg tatgtgtttg
      361 atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat gttccggaaa
      421 aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa aaactagatg
      481 aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc tatgatactt
      541 ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc ggctttggtt
      601 tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct gctgtaggga
      661 tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt accggagcga
      721 tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt ttagctttac
      781 cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca gaagaggtaa
      841 ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac tcgatcgtag
      901 cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg atgagcccgt
      961 ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt gcgacattcc
     1021 gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg atatatgcag
     1081 aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat tatttccgtt
     1141 ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat aaattgatct
     1201 tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt
     1261 acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga atcattgctg
     1321 ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc caatctggtg
     1381 cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt cttcttgtct
     1441 tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat gggatcggga
     1501 cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt
     1561 tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg ccaatcatgg
     1621 ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct tatcagcttg
     1681 gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt gtgctaggtg
     1741 ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa ggattcctgt
     1801 ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag tagtactgga
     1861 gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac cgtcatttta
     1921 cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga tcctatcatc
     1981 agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg
     2041 caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt ttggctgtag
     2101 aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca ggtgagggaa
     2161 aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga ggaggagaag
     2221 gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca gctggtgtaa
     2281 cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact gcgttgatca
     2341 gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa ggttcgtacc
     2401 gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca attgataaaa
     2461 ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca agttttgaag
     2521 agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga aaagctggga
     2581 tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag
     2641 aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc acaccagaat
     2701 tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc actgctagcg
     2761 aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa gcactgaaac
     2821 gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat ggacaaggaa
     2881 gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga agtgccaagg
     2941 cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct
     3001 tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg
     3061 aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat attgatacag
     3121 ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg gggactttcg
     3181 aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg ttctgtttgt
     3241 ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa gaggaggaag
     3301 cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag tcggtgaagg
     3361 aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag ggaaagcaca
     3421 atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa
     3481 gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa tcaagcaaat
     3541 aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt tgatcattat
     3601 cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa gacttcatca
     3661 cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt ttgttcatgg
     3721 gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag atttccagta
     3781 ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat agcgactcgc
     3841 tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc atcaattcct
     3901 acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt cttagtgatt
     3961 tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt gtaagcttgg
     4021 aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt cagtgaacga
     4081 tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag cgaaagaaaa
     4141 gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc ttcgaaattc
     4201 agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc caattgttct
     4261 gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt taatcttaat
     4321 atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac tccggctaat
     4381 agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc agaaacagca
     4441 aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc ttgtctgtta
     4501 cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc tgtctgttgg
     4561 tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga agcaactcct
     4621 aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat tttacttctg
     4681 gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat ggtaatcata
     4741 atgatcagag taagtatgat aataccggct acagccacta gtaaaacaac atttttagaa
     4801 aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc atttgattgt
     4861 aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc atccatttta
     4921 tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga tgtcccattt
     4981 agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat gaagttgaat
     5041 ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat cgtcatttca
     5101 taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac tgccaggtca
     5161 tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc gctgtcatct
     5221 attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt ttgtgcagtt
     5281 gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc gcccattcct
     5341 ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga atcagaacta
     5401 gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa ggaataactc
     5461 tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg tgtaagagaa
     5521 aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt ttgttcctgt
     5581 tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac ttctttttgt
     5641 gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag cacaaaaata
     5701 agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac ttttgtactt
     5761 ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg catttgataa
     5821 tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca tcaacatgag
     5881 gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa atgaaaaaag
     5941 tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat cctagatgct
     6001 acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc gatttagttt
     6061 atttgttgta taataatggg gaacagctga atacttatat aagtattctg gctatagcgt
     6121 ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc atccaactgt
     6181 cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag aaatagtgat
     6241 tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa tcgacatctt
     6301 taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat tatcccatta
     6361 ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga ggatattttt
     6421 attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga cgtcgtaatc
     6481 cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt tttaacacat
     6541 ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc tgtaccagta
     6601 ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa tgaagcagcc
     6661 aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt tggtgaaaca
     6721 gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat caatctgaaa
     6781 acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac ggctatccca
     6841 atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt cttagcctta
     6901 ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc ccaaatagca
     6961 gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc ttgtgtagca
     7021 agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca acgaaaagtt
     7081 gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt agaaaaactt
     7141 caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta tgatccagaa
     7201 gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct gcaaaaaatg
     7261 gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta tatcactact
     7321 acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat ttatcgagct
     7381 ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc caatcctaat
     7441 gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt tcaaggagag
     7501 tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc atacaaaaat
     7561 atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat gagtgttgca
     7621 ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga tatcggaaac
     7681 attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc ggttgtcact
     7741 atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg gggattcgtt
     7801 tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga aacaatcgtt
     7861 gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga catccgagat
     7921 caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt gccagtcatt
     7981 atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg aacctctcta
     8041 tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat acagcatccc
     8101 tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca aacaaaagtc
     8161 gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag tcatgtggta
     8221 gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt gatatgtaaa
     8281 actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc tttgaacgat
     8341 cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg ttttcctgga
     8401 atggattttg tattaatgca tacaggatat ccccacggag caaacacccc tattggaata
     8461 aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga aaaagaagaa
     8521 gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc cgatttagaa
     8581 aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag agaagctaag
     8641 cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca tcctgtatta
     8701 tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat atttagcagc
     8761 tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag atacagagaa
     8821 aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta acaattgaaa
     8881 tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca ttgacagact
     8941 taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat agtgaccaca
     9001 taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt attgttgaga
     9061 ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc ggtggaaaac
     9121 atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca agctatgaag
     9181 tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa gcagaaggaa
     9241 aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg cttgcagaag
     9301 aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg gggccagaag
     9361 atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc cattacaata
     9421 ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa gatagtgaag
     9481 gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc gacaaaacat
     9541 gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc tgtaagaagc
     9601 atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa agatgcagaa
     9661 aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga acgcaaatta
     9721 aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga aggggacgtt
     9781 ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg cgcgatgact
     9841 aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg caaccgggcg
     9901 gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg aggattcaat
     9961 acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt acgcacctct
    10021 tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca attgatcaaa
    10081 aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca ttatttgcag
    10141 cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca ttcgcgtttt
    10201 ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga tgttttaggg
    10261 aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag tgtaaagaaa
    10321 acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga agtcatgccg
    10381 gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt aatgaaagcg
    10441 atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca gatatctgga
    10501 aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc taagttcaaa
    10561 ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg cgtacttagc
    10621 gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg aaatatcttg
    10681 attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga attagacatt
    10741 cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga agtgtttatt
    10801 gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg ttcttagaaa
    10861 gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa tacgataaaa
    10921 tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa gtaggattag
    10981 ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt gaaacgattg
    11041 aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac caaggagcgc
    11101 tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga tatcagttgg
    11161 cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga gatcttgtgt
    11221 gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct ttgatgtttc
    11281 ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga atcgtcggag
    11341 atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt gcttcgatat
    11401 tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa atgccgatga
    11461 aagtcgctaa actttccggt tatctttatc aaaatatcca ggtggatgaa aatggtgcag
    11521 gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat tcagagacag
    11581 cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg atttttgttc
    11641 agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc atcaatgaat
    11701 tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat gcaaaagatt
    11761 tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg tataagagca
    11821 gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat cacaaatgta
    11881 tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt aaaggagaaa
    11941 gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg aagatcgagg
    12001 cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag aaactgtcat
    12061 agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg gcgatagcgt
    12121 gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac aaggaacaaa
    12181 agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg tagaaggatt
    12241 tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat tcaaggtctt
    12301 ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga gtgaaacgaa
    12361 aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg tcattcttgg
    12421 attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa aatacgatca
    12481 agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat tacaaagtca
    12541 acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata actacaattc
    12601 aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag ttgctgcgat
    12661 aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt atgtcccatt
    12721 gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt cgttcccctt
    12781 gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg ggattttcgc
    12841 tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta acgcgtctcg
    12901 gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag taccgatggc
    12961 aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt cgacattcct
    13021 gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa ataaagaagt
    13081 tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga ataccattta
    13141 tttggaataa
//
LOCUS       DAPCMU010000075        12999 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPCMU010000075 DAPCMU010000000
VERSION     DAPCMU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.078"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            96..1271
                     /gene="mltG"
                     /locus_tag="RD744_001738"
     CDS             96..1271
                     /gene="mltG"
                     /locus_tag="RD744_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDU0557732.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            1407..1886
                     /gene="greA"
                     /locus_tag="RD744_001739"
     CDS             1407..1886
                     /gene="greA"
                     /locus_tag="RD744_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDU0557733.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2055..2786
                     /locus_tag="RD744_001740"
     CDS             2055..2786
                     /locus_tag="RD744_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDU0557734.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2783..3850
                     /locus_tag="RD744_001741"
     CDS             2783..3850
                     /locus_tag="RD744_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0557735.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3857..4489
                     /locus_tag="RD744_001742"
     CDS             3857..4489
                     /locus_tag="RD744_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557736.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4629..5294
                     /locus_tag="RD744_001743"
     CDS             4629..5294
                     /locus_tag="RD744_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDU0557737.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5322..5615
                     /locus_tag="RD744_001744"
     CDS             5322..5615
                     /locus_tag="RD744_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDU0557738.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5683..6630)
                     /locus_tag="RD744_001745"
     CDS             complement(5683..6630)
                     /locus_tag="RD744_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDU0557739.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6808..7611)
                     /locus_tag="RD744_001746"
     CDS             complement(6808..7611)
                     /locus_tag="RD744_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDU0557740.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7757..8671
                     /locus_tag="RD744_001747"
     CDS             7757..8671
                     /locus_tag="RD744_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDU0557741.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            8719..9189
                     /locus_tag="RD744_001748"
     CDS             8719..9189
                     /locus_tag="RD744_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDU0557742.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9274..9882
                     /locus_tag="RD744_001749"
     CDS             9274..9882
                     /locus_tag="RD744_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDU0557743.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            10016..10216
                     /locus_tag="RD744_001750"
     CDS             10016..10216
                     /locus_tag="RD744_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0557744.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            10970..12196
                     /locus_tag="RD744_001751"
     CDS             10970..12196
                     /locus_tag="RD744_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDU0557745.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(12304..12774)
                     /locus_tag="RD744_001752"
     CDS             complement(12304..12774)
                     /locus_tag="RD744_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDU0557746.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         4490 a         1979 c         2631 g         3899 t
ORIGIN      
        1 tttttgatag aatatttaaa taaaatcatt cacgccaatt caaatatcat gtataatgaa
       61 taaagttacg aacttgatag taaggaggag ttgttttgtc aggaaacaaa caacacccaa
      121 gtaaagataa aaaagaagcc agtaaggcag ctacagcaga tataatcgac aagcggagaa
      181 atctgcgtaa aaaagaagat aagattgtga ggaagatcat tttagtgatt gctcttacat
      241 tattgattat tggcggattt cttggattta ccgtgtatcg ttatgtagat agcgggttga
      301 agcctttaga taaatcagat gaccaattag tccaagtcga gatcccgagt ggctcttcta
      361 acaagcagat tggtgagatt ttggagaagg acaatattat taaaagcggg attgttttca
      421 attattatac aaaatttaaa aatttaactg gctttcaagc tggctattat cagttagcgc
      481 ctaatatgac tcttgacgag attggcaaac agttgcaaga aggcggtact tctgagccaa
      541 caaaagttgc agatgggaaa attgccattc cagaagggta cgatatcgat cagattgctg
      601 aacgggtagc gaaggtaact ggtaaagaca agaaagaatt tttagattta gtgaatgatg
      661 agactttctt taacgagttg caccaaaaat tcccagaact gttggaaagt gcatctaaag
      721 ctgagaacac aaaataccgt ttggaaggtt acttattccc agccacatat gattattaca
      781 cgagtacttc gcttaaggat ctagtgattg aaatggtgaa caagacgaat acggtcatgc
      841 aaaactatta ttcagctatc aagcagaaaa atttgactgt acaagaagtt ttaactctag
      901 cttctctagt tgaaaaagaa ggcgtaaaag aaaatgatcg taaaaacatt gcacaagtct
      961 tctttaaccg aatcaaagca aatatgccac ttcagtcaga tatctctgtt ttatacgcat
     1021 taggtgaaca caaagaatta gtgacgtatg aagatacagc tgtagattca ccatataatt
     1081 tgtataccaa taccggctat ggacctggac cttttgacaa tccaagcgaa gaggcaatca
     1141 aggcagtatt agaaccagca gaaaatgact attattattt cgtagcagat acgagcactg
     1201 gaaatgttta tttcgctaaa acttatgaag aacatatgga attagtggaa aaatatgtaa
     1261 ataattctta gaaaaacagg ctctagacat tgatcatttt tagaaaaaca tttacatttg
     1321 aagaaaagcg tggtaatctt ttttggacta tattcctcaa aagattacct tttgcggtat
     1381 tttatcgaaa aaaggagacg ttacacatgg tagagaaagt ttatccaatg actctagaag
     1441 gtaaagaaaa attagaaaaa gaattagaag aactaaaaac agtcaaacgc ggagaaatca
     1501 ttgaacgaat caaaattgcc cgcggtttcg gagatctttc cgaaaactct gaatatgaat
     1561 cagcgaaaga tgaacaagca tttgttgaag gaagaatcac aacaattgaa aatatgattc
     1621 gttttgctca aatcatcgat aacgacggtg tagatgcaga tgaggtatca attggtaaaa
     1681 cagttacatt catcgaacta ccagatggag aagaagaaga gtacacgatt gtcggaagcg
     1741 ctgaagcaga tccgttttct ggcaaaattt ccaatgattc tcctattgcg caagcattga
     1801 ttggcaaacg taaaaatgat gaagtaacta ttgctactcc aggtggcgat atgttagtta
     1861 aaatcgtcaa agtagaacaa gcgtagtctg actgaaagat ctagagagtt catcttactg
     1921 atgatacact ctagatcttt ttctctttgt gtattttaga tttcaacaaa cgatcttaca
     1981 tctaaagtcc tatagccaac agttaaaaaa ataggtatga tgaaatgaga atgtgtcagg
     2041 agggagaaag agcgatgaat agttcttggc gtttttttgt ggtagttgaa gcactactgc
     2101 tgttatttgc tgtttggcaa atcgtaaaca atactggact tttgctttta gtgatattcg
     2161 gtattttcaa tatatatcta gctatgcgca aacatcctcg tactaaattt caaaattttc
     2221 aattgatatt aggaagtttg gtcatttttt ttagcttagt caacagtcct gctttatgga
     2281 tgatggctgt atttgccgtt ttatttattg gtctgaaagg tgtagagatt tcagggatag
     2341 atttaaccaa aaatgctttt tggcgaaaga aacagatcat gatgatccaa acagagcaat
     2401 taaaaacaca taataatgag cggaaaagac agcaattatt cggtaaccaa cgtattggaa
     2461 acgacgtcta tgaatgggac gatatcaaca tcgccgtcat ttctggtgat actatcatag
     2521 atttgggcaa tacgcttttg ccaaaagatg ataatattgt gattgtaaga aaagggatag
     2581 gcagaacacg gatcttagta cctttgggcg tagccatccg attagagcat gcgacattag
     2641 taggaaatgt cttgtttgaa gaagagcaat tttctttgaa aaatgaacaa atcaaaatct
     2701 acagcagtga ttatgatgaa aaccctcgta gattgaagat tattactaat accttgattg
     2761 gagatgtcga ggtgatacga atatgatggg aaaaatatcc agagcgatgc tagctgttta
     2821 ttcggggatt gcggctttcc ttattattct attttcactt ttcacttatt tttatgccag
     2881 caatcaaagt cattggtggg gagaattact gcgtgcacgt ttattatatg ttccgcttat
     2941 cttccatttg ttagccattt ccttaggtgt aggattaatt gtctttctat tattatcact
     3001 tattcaaaag acaaaatacg ggaaaatcga agaaaagctg cgtgcacttt cttctggcaa
     3061 ttatgaatcc aaattgttgc ttcttccgat cccaagtgca tcggacgatt tatacatcaa
     3121 agatattgat aaggaaatca ctaagataaa agaaaaaatg attgaagtat ctagtgaatt
     3181 acaaattgta acgagccgtc cccaatatgt agatggacaa accaaagaag aaattttaga
     3241 attagaaaga catcgattag cccgtgagct gcatgattca gtttcgcaac aattgtttgc
     3301 agcaatgatg atgatgtcgg cattgacgga gcaagcagaa aaaagcgaga caccagaaat
     3361 gtttcgcaag cagttgaaaa tggtagcaga gatcatcaac gcttcccagt ctgagatgcg
     3421 tgcgctgctt cttcatctac gtccagtcaa tttagaagag aaaagtttaa agcaaggtat
     3481 cgagcagtta ttgaaggaat tgcaaaataa aatccagatt tcgctgaaat gggatgtaga
     3541 agatgtaaaa ctatctagtt ccattgagga tcacttgttc cgaatcgttc aagaattgtt
     3601 atcaaataca ttaagacatg ctaaagccaa tgaattagag gtatatttgc acaaaataga
     3661 caataatctt ttgttacgta ttattgacga cggaacaggg tttaatatga atgaaacgaa
     3721 aacgggaagt tatggattga acaatatcaa agaacgagta gctggtattg gtggaacagt
     3781 aaaaatcatt agttttaaag gtcaaggtac aagtgtagaa atcaaggtcc ctttgatgaa
     3841 ggaggcatag ataaggatga taaaagtttt attagtagat gaccatgaaa tggtgcgctt
     3901 aggcgtctct tcttatttat ctattcaaga agacattgaa gtaattggag aagctgaaaa
     3961 tggacgacaa ggctatgaga aggcgatgac acttcgaccg gacgtcattt taatggactt
     4021 agtcatggaa gaaatggatg gtatcgaatc aacaaaagca atcttaaaag attggccaga
     4081 agctaaaatc attatcgtaa ccagttttat tgatgacgaa aaagtttatc ctgccattga
     4141 agcaggtgca gcagggtatt tgttgaagac ctctaccgca catgaaattg ctgatgctat
     4201 aagggcaacg caacgtggcg agcgagtatt agaaccagaa gtgacaacaa aaatgatgga
     4261 aaaaatgagt cggcgaaatg agcctgtatt acatgaagaa ctaacgaatc gggaaaatga
     4321 aattttaatg ctgatttctg aaggaaaaag taatcaggaa atagcggacg aattgtttat
     4381 tacactgaaa acagtaaaaa cacatgtttc caatatattg gctaagctag aagtggaaga
     4441 ccgtacacaa gccgctatct atgcattcaa acatggtttg gtgaaataaa aacgatgata
     4501 aagaggataa tcaaaagctg agaaaataac tattattttt cttggctttt ttacaataat
     4561 gatgcttgct tttgtttttg taaaggcttt gttatactga gaaaagaaga tactaaagga
     4621 ggaaaacggt gaaacaaaat tttgcaattg taggtcttgg acgatttggc ggaagtatct
     4681 gccgaacact gatcgaagct ggtcaagaag tactggctat tgatagtaat gaagatcggg
     4741 tcaatgaata tatgaatata gccactcatg ctgttgtggc aaatgcacag gatgaaatga
     4801 cacttcgatc attaggcata agaaatttcg atcatgtaat tgtcgctatt ggagaagata
     4861 ttcaagccag tatcttagtt actctgatgg taaaggaaat gggcgtacca aatattttag
     4921 caaaagccca aaatgagtat catgccagag tacttgaaaa gatcggagct gatcgcgtcg
     4981 tccatcccga gcgagacatg ggaataagga ttgcgcataa tttagtgtca aagaatattt
     5041 tagattacct agagttgtca gataaatatt ctttagcaga aatccgagtc tccaatccta
     5101 aatttttcaa caaaacatta gctgaattaa atttcaggca acgttttgac ttaacagtag
     5161 tcgccatacg aagagtagat caaacggtta tcgcttctcc tgcagcagat gaatatgttc
     5221 gtgaaaatga taatctactg gttattggag agacaggcga tgtggatatt ctagacgata
     5281 agatgaatca atagaaagaa aagagaaagt aggttgttgg gatgaaatta acggtaacgc
     5341 caaaagcaca aaagtggttt gactcagaaa ttgatctgcc agaaacttac ggaatacgtt
     5401 tttttggaaa agtctacggc aaaacagagg ttcatgatgg tttttcgatt ggaatgtcag
     5461 ttgaacagcc agaacatcct gtaaagaaag tggaaatgga tgggacactt ttttttatcg
     5521 aggaagcaga tgaatggttc tttaaaggat atgatttact tgttgattat gatcccaacc
     5581 tggaggagcc aacctatcac tttaaaaaac aataaaaaaa gagagggtga cacaattttt
     5641 gtcaccttct cttttttctt ttgaaaatat tgttttcagc tattaatatc ctgcagacaa
     5701 ttttacttga ttggtttgaa gctcatgggt ggcaaagtag cttttcagat tggttaaaaa
     5761 aatagccatt aatttctttt gaaaatcagg tgtcaaacct gcaatgtggg aagtaatcag
     5821 tacattgtcc attttccaaa gaggatgatc ttgtggaaga ggctcttcct caaaaacgtc
     5881 taatgctgca aaggaaagtt gctggttatt taaagcctgc accagatctt gtgtcttaac
     5941 agaagcacca cggccaacat tgatgaatac agattttgga tcaaagtgtt caaacatatg
     6001 ctgatcaaag agcccagttg tttcctccgt tagaggtaag atattgacta cgaaatctac
     6061 ttcaggaaga acttccttta gttgatttaa agaaaaagtc ttttccattc cttctgctgg
     6121 atgaccggat gtattgattc catagcagtt catgtctaat gctttgcatt tttttgcaat
     6181 catttttcca atatggccag ttccaatgat tagtattttt ttcccttgga tagattggta
     6241 atggaccgat gaacctaacc atttcatgtc actctgtgcc ttttgtgctt gaaaaagacc
     6301 acgagaatac ccaaggatga ttcctaagac atgctccgta atcgattcac tgtggatgcc
     6361 actaccattt gaaagtaaaa tatgtttctt tgaaaaagtc tcaaggggta gctgatcaac
     6421 accagctgag atagattgga cccatttcaa ggtagaagag gctaatagtt tttcttggta
     6481 gtctgagttc cagcccaaac tgatctctac attttgtaga tcctctggtt gtaattctgt
     6541 tttaaagata taaccaggcg ctatagtatg gatttcttcg ataaattcat cacgaaatgg
     6601 acgatctgca taaatgattc tttctgacat agttaatttc ccgcctttct ttactctaag
     6661 tgtagcgcat acaagaagaa ctttgaagcg ccttgcttgt tttttgagaa agaaatattt
     6721 ttcaaataaa aaaggggctg tgacaaaaaa atgtcacaac ctctaaaagg gtctattgaa
     6781 gtagaaataa cgagttgtct ttatttctta attgatcgat atttttgccc atcctttgac
     6841 agcttgagca aaaataaaga ttcctgcaaa agcaatgaag gcgctagtgt caaaagaagg
     6901 aagaaagacc atcaagattg ttgcaattat agtaggcaat gaaattgaat agatcaatgt
     6961 ttttaaacaa tcaagaaaag tagcttttgt tagtcgaaag cgtgcataga gaaaagcagc
     7021 aatgttggta aatagcaaag tgatcagcag attcagaaaa ctaggataaa tagaaaatat
     7081 aaatgtcagg acttttatcc aaagtggtat agatgcttca cttaaaaaag aacggagctg
     7141 cttacctgtt aagtttttta aagattcatt ggtgtaggga agttctaatt ggttattgtt
     7201 caacaaagca gaagtcgtgc ctgtattagg aagagctatg ataagtttgt tttttaacat
     7261 cccgacacta acaaaattcc ccatcaaatc agtagagatg tcctgttctg ttcgttttcc
     7321 ctctggatca aatgtgaaga taatggagtt tgtttggtaa atgaatcctt ctttttcttc
     7381 ggtttgaagc tgcccgtttt ggattgtaaa atcaggaatg cgagtagcaa tcttttgccc
     7441 atccaattga atgttgttta agacttgaaa aacttgaaaa gagattggca aagccataat
     7501 tatactcaaa actaaaagat agacaatggc tttccaaaat gggacatttc tcgcctcctt
     7561 taggtctttg aatcgtataa aggaactgat aattaattgt tttgttgtca taagtactcc
     7621 tcatctagta gtgtcatcac ttgcaattct agcgaagtat caagtgaaat acaagtgctt
     7681 taaggaaaca gctgaatgat tggtgaaata tcaaaaaaat gttattcttg tctaaagact
     7741 atccaatata aataaaatgg aggaaggaat agtgcctaca cagccacaga gatcatcaaa
     7801 aaaactattt ggagtagtgg ttttgaatgc ggtgatcact ttatcagaat ttattggagg
     7861 gattatgtca gggagtttgt ctcttgtttc agatgctttc cataacctta gtgatacatt
     7921 ggcaattatt ttttcttacg gggcacaaaa aatggcccga aaagaagcaa ataaaaaacg
     7981 tacgtatggc taccagcgtt tagaaatatt gagtgctttc atcaactcat tcatattgat
     8041 catattatcc ttgtttttaa ccgttgaagc attcaagcgg ttcacttcac cagaaaagat
     8101 aaatagtcat ttgatgctaa cagtagcagt tattggattg ctcgcgaatt tgttctcagt
     8161 attattatta cgacaagaag cagatgaaag tttgaatata aaatctagtt atttgcattt
     8221 gttaagtgat acactgtctt cggtaagtgt gattatcgga gcaattttga tccgtttttt
     8281 cgatatctac tggatcgatc cagttattac actgatcatt tcgatatata tattgataga
     8341 agcgataatg gtgatcaaaa aagctgccgc tattttgata caaagtgcac ctgctatcga
     8401 ctatgaaaaa atggaacaag aaattgaagc aatcgatggg gtaaaggatg ttcatcatgt
     8461 ccatatttgg cagtatagcg aaaagataat tattttcgac ggacatattg attttgaaga
     8521 tcagctacta agcgagattg aaaagatata tcctcgtata atttctttat taaaattaaa
     8581 atatggtatt acacatgtga cgatccaagc agagacaaag gcaaaggatc aaaaaaaatt
     8641 aattttttta aataaagatg aaagttattg aaatatagag tatgataaat aagaattttt
     8701 aataaggtag gttggctcat gaagcagtat cgacagttaa aaggatattt agaagcaaaa
     8761 ggatattggg agatactagt ctatctcttt tttggtggtt tggctacgat tgtaaatttt
     8821 gttagttttg ctctcgcacg ccaatatttt gatttaagta tggccttatc caattctatt
     8881 tcgtggtttt gttctgtatt gtttgctttt gtaacgaaca aactttgggt attccattca
     8941 aaatcaccga attttacaca tgcacttatt gaatgcggta agttcttctt ctaccgtatc
     9001 ctttcttacg gattggacat gggggcaatg gtattgttga tcaatgttat gaacagcaat
     9061 gagtatgtgg cgaaaatcat cactcaaatt attgttatcc ttgcgaacta tattttcagc
     9121 aagttgttta tttttaaaga aactgaggtt tttgaagaag aagtaggtca aagtgaaaaa
     9181 gacgactagg cataacaatt gaagaatttc cttctctttg ttactctttg taatgtagac
     9241 attattaaga aaacattgag gaggaatttt tttatgactt acacattacc agatctacct
     9301 tatgcatatg acgcattgga accttatatt gacgaagaaa cgatgcatct gcatcatgat
     9361 aagcatcaca atacttatgt gacgaattta aattcagcaa ttgagaaata cccagaatta
     9421 ggcgaaaaaa caatagaaga attattatct gatatggacg ctattccaac agatatcaag
     9481 acagctgtac gtaacaatgg tggcggacat gctaaccatt catttttctg ggaaatcatg
     9541 gcaccaaatg ctggtggcga acctacagga gaaataaaag aagcgattaa tgaagctttt
     9601 ggtgattttt cttcttttaa agaagaattc aaaaaagcag ccgctggacg atttggttct
     9661 ggatgggctt ggcttgtaat ggaaaatgga aaattagcta ttacctctac tgcaaatcaa
     9721 gattctccat tgatggaagg aaagacacca attctaggtt tggacgtttg ggaacatgct
     9781 tattatttga aatataaaaa tgtccgacca gattatattg cagcattctg gaatgttatt
     9841 aattgggatg aagtaaacaa acgtttcgaa gcagcaaaat aaccatttcc tccgtcatat
     9901 cagtcttaaa aataatttgg aaaataaaag agcgaaagct tgctagcgtt ttctaaattg
     9961 ggtataatgt tcttgcaagg ttacttgcaa gaactttttt ggaggatata catttatgga
    10021 acacggaacg gttaaatggt tcaacaatga aaaaggattt gggtttatta cagtagacgg
    10081 cggagatgac gtatttgttc atttctcagc aattcaagga gacggcttca agagcctaga
    10141 agaaggtcaa gaagtagaat tcacgattgt tgaaggtgca cgcggacctc aagctgctga
    10201 agtgactaaa ttataataat ttgttgaata aagacacgac ttcttagaga gtcgtgtctt
    10261 tttgtctatt tatgaagttt ttagattttt tggctaagta aatgcatttg ttcacttgac
    10321 atgtcctaac taattatcaa gcaaggaaca ttttcttcgt tagtaatgga ttttaaagaa
    10381 atttcagttt ttggatgttg aataaaagac catttgtgaa accactagta tacatactca
    10441 ttaccatgtc aattctatta ctttaaaaga aaatcgtttt actttgaaga ggagcatatc
    10501 tatccattaa aagatgatgg taatgcaatc actagttcga gtcattttaa gagccatgca
    10561 gtgagctgga aataaaacga atagaagatg ggtttaacaa aaggaccagt cataggaaga
    10621 aattgtcgtg aaatcgaatg ataatctgaa cggtaggcaa agactgttaa aaagatggga
    10681 aaggtatgtg cgatcatttt actgcacgat tatttggaaa ataccttgtt gttcgctttt
    10741 tctggaaaaa atacataaaa aaagataaaa cgattgcgaa ctgcgtgtat tcggtctttc
    10801 gatctttttc acggtatggt atgatagtta agaattttga gaaattttaa aaaataataa
    10861 tcgttacaca aaaatgtttt ttaaagtttt cttatgatta aaaagtgaat ttgctgattg
    10921 aacagttata tgagacaata ttttagaaaa tgtgaaacag aggtgtatta tgaagcgatg
    10981 gcaaaaagtt ctattaacct tgttaggggt attcgttttg ctgattgccg gattatcagc
    11041 atacggaatc agatttatgg gagaagcgag ccaaacagta aacaaaattt ctaaaaactc
    11101 taatcgaata tcctccaaac gttctgagag agtaagcatt gatgacaaag aacctttttc
    11161 ggttttgcta ttaggtttag ataccggtgg attaggaaga acggaacaag gacgttctga
    11221 tacgatgatg gtagtaacag ttaatccgaa acaaaaaaaa tcaacgatca tcagtttgga
    11281 ccgagatatt tatacgaata tcgtaggtta tggaacggta gataaattaa atcatgccta
    11341 cgcatttggt ggtgtcgaaa tggccatgga ttcaattgag caactgctcg atattcctat
    11401 tgatcattat gtaacgatca atttggatgg catggaagat ttgatcaatg cggtcggtgg
    11461 catcaaagtg aataataaaa ttgactttac tttggatggt gtccatgtac cagtaggtga
    11521 acaaacattg aatggagaaa aaggtttagc ctattctcga atgcgacacg aagatccaga
    11581 aggcgatatc ggtcgacaac gtcgtcagcg ggaagtagtg acgaaaatcg tcaataaagt
    11641 tttaagttta gatggcgtaa gcaattatag aaaaatttta aaagcagttg agaacaatgt
    11701 gacgacggac ttagattggg atgacatgct tgatgtagcg acgaactaca ccccggcgtt
    11761 tgaaacaatc aaacaagacc agttgcaagg cgaaggacaa atgatcaatg atatctacta
    11821 tcaaatttta ggaaaaaatg atcttctttc gatccaaaat gaactgaaga aacaattaaa
    11881 tataaaaaca agcgatacat tacctaattt gaaaaatgag aatgcttcaa tgatgttctt
    11941 tgatgattcg gaagatggcg attcgacaaa cggtgatgga tcgaatagtg aagggtacac
    12001 cgattattct tcctattcag aaaacacata tgatcagtac caagatccga atacctatta
    12061 tcagcaaaca gaagatacaa gtcagtacta tacaccaagt caagatactc aaagttatga
    12121 tccttacgct ggacaggaac aaaatggtta tggttatgac cagaataatg agtatggaac
    12181 aacaagtggg tactagaaaa aaatagtttg gttaataaag agtgccacct aaattagaca
    12241 aaaaatctaa cttaggtggc actttttcta tgtgttaaat tacaggacgt ctctttttag
    12301 cgcttagttt aaaaaataaa ttgaataatt caatagagtt gcgaatagta cccataaata
    12361 ataaggaatc atgagccaca ttgtcaggcg atgatgcagc cacaactgat acataagcac
    12421 tgacaagaaa ataactaaaa gtgtaatatc gattgctgca atgaaaatat ttcttaaatt
    12481 aaaaaataga gcagaccata taaaattaaa aatgaactga atcacaaaaa gaccgacaag
    12541 attgtttcgc tgttttttgt tgttgtgatt aagtaacaaa taaagagaaa tacccatcaa
    12601 tatatacagt aaggtccaca tcggcccaaa tatccagcta ggtggtgcaa atggtggaag
    12661 ttgcaaagaa tagtaatatt ttccgggatt tccgctaagc aaccctgata aaaagcctaa
    12721 tatgacaatt cctataacac aaacccaaaa acgaatatct tttatcatct tcattttact
    12781 aactcctttc tataatatta gtttaccatg aaggctcaca gcaagacctg tatttagttt
    12841 gtgaagtaag aaaaaaattg ataattgttg ttattaaaaa gttgaacatt tgtttatatg
    12901 aatgatgttt ttgaaaaaga tcatatgatt tgattctcat gttttctatt tgtacttttc
    12961 tcgtctactt ctgttataat ctgcatctta cttttttca
//
LOCUS       DAPCMU010000076        12961 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPCMU010000076 DAPCMU010000000
VERSION     DAPCMU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.079"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            139..477
                     /locus_tag="RD744_001753"
     CDS             139..477
                     /locus_tag="RD744_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557747.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="RD744_001754"
     CDS             complement(682..1257)
                     /locus_tag="RD744_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDU0557748.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="RD744_001755"
     CDS             complement(1989..2570)
                     /locus_tag="RD744_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0557749.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="RD744_001756"
     CDS             complement(2753..3379)
                     /locus_tag="RD744_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0557750.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="RD744_001757"
     CDS             complement(3401..4732)
                     /locus_tag="RD744_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDU0557751.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="RD744_001758"
     CDS             complement(4887..5288)
                     /locus_tag="RD744_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDU0557752.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="RD744_001759"
     CDS             complement(5374..5580)
                     /locus_tag="RD744_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557753.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="RD744_001760"
     CDS             complement(5675..7177)
                     /locus_tag="RD744_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDU0557754.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="RD744_001761"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="RD744_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDU0557755.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="RD744_001762"
     CDS             complement(7748..8365)
                     /locus_tag="RD744_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDU0557756.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="RD744_001763"
     CDS             8586..9959
                     /locus_tag="RD744_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0557757.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="RD744_001764"
     CDS             10034..11146
                     /locus_tag="RD744_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDU0557758.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="RD744_001765"
     CDS             complement(11218..12855)
                     /locus_tag="RD744_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDU0557759.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3506 a         2749 c         2311 g         4395 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 a
//
LOCUS       DAPCMU010000077        12885 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPCMU010000077 DAPCMU010000000
VERSION     DAPCMU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.080"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(51..296)
                     /locus_tag="RD744_001766"
     CDS             complement(51..296)
                     /locus_tag="RD744_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557760.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(290..691)
                     /locus_tag="RD744_001767"
     CDS             complement(290..691)
                     /locus_tag="RD744_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0557761.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(863..2671)
                     /gene="uidA"
                     /locus_tag="RD744_001768"
     CDS             complement(863..2671)
                     /gene="uidA"
                     /locus_tag="RD744_001768"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDU0557762.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(2699..4465)
                     /locus_tag="RD744_001769"
     CDS             complement(2699..4465)
                     /locus_tag="RD744_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDU0557763.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(4478..5272)
                     /locus_tag="RD744_001770"
     CDS             complement(4478..5272)
                     /locus_tag="RD744_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0557764.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(5283..6659)
                     /locus_tag="RD744_001771"
     CDS             complement(5283..6659)
                     /locus_tag="RD744_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0557765.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(6788..7435)
                     /locus_tag="RD744_001772"
     CDS             complement(6788..7435)
                     /locus_tag="RD744_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDU0557766.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(7519..8946)
                     /gene="uxaC"
                     /locus_tag="RD744_001773"
     CDS             complement(7519..8946)
                     /gene="uxaC"
                     /locus_tag="RD744_001773"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDU0557767.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(9020..10099)
                     /gene="uxuA"
                     /locus_tag="RD744_001774"
     CDS             complement(9020..10099)
                     /gene="uxuA"
                     /locus_tag="RD744_001774"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDU0557768.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(10102..11733)
                     /locus_tag="RD744_001775"
     CDS             complement(10102..11733)
                     /locus_tag="RD744_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDU0557769.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            12050..12721
                     /locus_tag="RD744_001776"
     CDS             12050..12721
                     /locus_tag="RD744_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0557770.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         4084 a         2538 c         1697 g         4566 t
ORIGIN      
        1 actattagaa gattctttta aacagaataa gtggaaaggg gactttttag ttagttaatt
       61 ttaaatttta agatcgcttc tatcaattta atttctagct gtcttagcat atactcatct
      121 acataggtaa taaattgccc cgcctcatta aatccatttg tcaaacataa cttagctata
      181 taactatgat aatgtttgag tatcatcttg attgaaattg gatcttcttc tagagcagat
      241 tggataatgg atagaggaac taatggatac tgccgttttt tggggttgtc taccataagt
      301 cataccccat ttttaattgt tcaactgcct tctttcgttg ataccagata ccacttgtac
      361 tcatattgag gattttaccg atttcagtat cattaaaacc tgcaaaataa taaagtagga
      421 taacctttct tcttatttcc gataaatgat caatggctgc cgataacttt tgatctgaaa
      481 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggagt
      541 acttaccttc atttgaattc gtcaatggtt cctcattatc tgttatgtta atgtttcgtt
      601 ttcgaataga cttagtttcg tttcgtaata cttttttaca gtaggcatca aattgatgtt
      661 gtatagattt ctcattgtaa ttttgttgca tgataaatat gctccttttt tcccctttcc
      721 actaatacaa caatcagaaa gcgccttttt ttcgaaaaaa ctagaaaaaa atatccttct
      781 ttaaacagaa aatcttttta taaaaaaaag gagttggcaa ttaagcagcc actccttttt
      841 tatattcctt ataaaaggta aattattttt tataactgaa atttggtatt gatgaccatc
      901 gtttctttag atagtgagca atcattttag gctttctatc tctagtaaag attcctttct
      961 tatttccttg cactcttaaa atcccttgac ttgtttcaaa atctgcaaaa ttccacactt
     1021 gttcaccaac aaaattgtca catttatcta ttacttcgtg atttgcttcg taataaccca
     1081 cttgatactc ttctgtaaac attgttggta ctgtatcatg aagtccagct actgtatctg
     1141 ctccatattc tgtaaacatt attggcgtat cgggacatct ttccatccat ccatctagct
     1201 cttttttcaa cagatatttt gcatgtttta ggtctccacc tgaaacatac caaccataat
     1261 agcgatttaa acataaaaca tcgacaatat ctcctacttt acaaatatct ggtgttgact
     1321 ttaagtaagt aacgattgtt acaggtctct tctgagggtc aagttcttta gtcaattcaa
     1381 ctaaaggttc aaaatattct ttagcgcctt cagaatccga atctggttca tttgcaatag
     1441 accacattac gacactcgga tggttcttgt cgcgttctat caattcctta atcgccattt
     1501 tgtgagcatt ttctgtccgt atttctttcc aagtatcatg tttttctacg ttgtcatcta
     1561 ataaagtagc catgaaattc aaatgtaatc ctactgcagg tacttcatca ataactacaa
     1621 taccttctcg atctgctaag cgcataattt cttctgaata aggataatgt gaagtcctaa
     1681 atgaattagc accgatccat ttcattaaat taaaatcttt gatatttact gcttcattca
     1741 aaccacgacc attaatatac gtgtcctcat gcttcccaaa tcccttaaag taaaaaggtt
     1801 tattattaat caaaaacttc ccattcgaaa cctgaatact acgaatgcca aattcttctg
     1861 tgtacgtatc aatcacttta ttttcgttaa ttaaactaac tttcatacta tataagtaag
     1921 catttagagg ctcccaaagt gttgggttct taagttgaat cactccttct ttccccttag
     1981 cttctgctat aagttgagct tcttcgtcat agattgctat tagaatttct ccggtccatt
     2041 ctgtatcgat attaaattct acatttgcat ctgaatcaat tatttttgta ttaatagtga
     2101 catcctttat atagtctttc ggagttgtat acaatttaac gggtctatta atgcctgcat
     2161 aattgaaaaa gtcaaaatta accaaatttt ctttttttat gtctccatct tcctgtatcg
     2221 tttctttcaa aatccctaca ggtaaagtgc tatcgtcaat aacattgttt acagccactg
     2281 taattctgtt acttccatct tttataaact gattaatttc tgattcaaat ggcgtaaacc
     2341 ctcctttgtg ttgattaatc aattttccat tcaaaaatac ttttgcttca tgtgttgcag
     2401 atccaaaacg aagaactaat ctttcttcta ataatttttt aggaagaatc acttcttttt
     2461 cataccacac ccatccaaca tgatttctaa tgttctctgt ttcaaataaa tcattataag
     2521 aagaaggaac tgaaattgga taactctctt tttcaaccat tggttctaaa gcaagttctt
     2581 cttgtaagtc atcatttgta tctaacttaa atctccaaag accgcttaaa tcaattactt
     2641 gcctcgtctc tgtcataatg ggatataaca tgtaaatcac tcctatataa tttaaatatt
     2701 aaataaatgt atcctttaac ccacaataag catctattgg acttattccc ttaaattcag
     2761 aaaggcccat tagtttgtcc actaatattt ctaaagtttc ttgttttcca tcataagtat
     2821 tgatataagt ttttacttga ggcacatcag ctaaatgaaa cggacactgt acagatacaa
     2881 aaattgttgg aatttcatga atataaaatg gaatatccgg agtaccttta cttgctggcc
     2941 aaacaattct ttgaactgta cctgggttaa cattagctat attaataatc aagtcatatt
     3001 tttcagttaa atctttgatt ggtctcttag cagcatatat tccagctata tttttaattt
     3061 tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt tcaacttcaa
     3121 atccttttgc tgacaaaatc tgtttcaata aatcaatggc ctgaggtcta tttccagcaa
     3181 ctacttcacc aaacccacct tttaccccat gtacatctac cagtaaaatc cttttatatt
     3241 tttctggtac aataggccaa atatcttgtt tatctttaac tagtgtaatt gcttgatcag
     3301 ctacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccata gcttcttgtt
     3361 tcggtaacaa aattttattc ttttcttttt tatgcaaacc tagtttagcc tttaagccta
     3421 aaattcttct taatgcatca cgtaaacgct cttctgaaat aattcctttg taataacctt
     3481 ccatcatata attaaagtct tcatctggat cattaaaaaa taggaataga tcacatccag
     3541 ccgcaataga agtgggcaat aattctgacc gtttcattgc cccagtcatt cctaacatat
     3601 gactagcatc agtaactact aatccattaa acccgagttt tcctctaagt aagtcagtga
     3661 ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca agattaaaat
     3721 gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat ccagcttcta
     3781 ttaagcctga gtatactttt ccaaaagaat tatcccattc ttctgtactt aaactattaa
     3841 tactaaaaga tagatgttga tctcgttcgt caataccgtc accagggaaa tgtttagctg
     3901 caggcaaaat tccactctct tgaatacctt tcatataagc taaactaagt tctaaaactt
     3961 tatcagggct ttgtccaaaa gatcgactag aaatgatagg atttctccaa tttctattaa
     4021 tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca attccagaaa
     4081 ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt acttctacac
     4141 caacttcggt accatctgta caagctccgt taccacctgc ttctgtatta gcagcaatta
     4201 ataaaggaat tttgctttct gtttgtagga tatagttttg atcatatacc tcatcagcag
     4261 ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatacta cttaagtaat
     4321 ttttatcacg actagatccc atattaacaa ataattgacc tattttttct tctaaagtca
     4381 tagcatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatga tatggttttt
     4441 ttcgtaagtc tacttttata gtcattttct ttctcctcta cttattagta agatttttcc
     4501 aaaaagtatc tatatatttt tgttcaatat ctatatactc tcccgataaa catttaaata
     4561 aaatatcagt tcttaagttt tcccatgatt ctttttcttt attaaccaaa ataggataaa
     4621 agaaaacttt attcttattt gcagcttcta gatcaccagg agaatcacca atcattaata
     4681 tcttatccaa atcacaacca ttttcaatta cacttcgaat aatgtcttct ttttttcctt
     4741 tatcttgaca acacagttca tcaactaagg ataataattc atgtctttcc cattcttctt
     4801 gaatagcttc cttattagct gagcttacaa taattatctt cccatatttt ttcagagctt
     4861 ttatagtatc tctagcactt aaaaagggtt tatctaatcc tttagcttga ctaatctgtc
     4921 gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga gagtattcta
     4981 tttccctctt caaagatgga ttagacaatt cttttgttac agatacccag tgctttaatc
     5041 gattaatcga tggtacatca tttttttcta atgttttaac taaacctaaa aatctgttta
     5101 ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat ttttccctat
     5161 gatgaacatc aaaaatctct gcagcaattg gcccgaaaaa gagattgtgt ttataagtca
     5221 ttgtatccat gacacaacca tctgaatcaa tacaaaatat ccaactgttc atgttttttc
     5281 tcctagttat gtttttgatt tagcaaatta taattttcta atactttttc tttagataat
     5341 ggatatacga aaaataaaat caatcctaca acaaagaaac aaactgctgg aatcaacgtt
     5401 gaagagttat acagcttttg taaaacttct ccactttgta cttttgctaa cgaatcatac
     5461 cctattatag aaagagtcca acctccgaca ccgccagcta aagcttgtcc aatttttcgg
     5521 gcaaaagaat atactgcata aatagttcca tcttctcttt ggccagattt cacttcttga
     5581 tcatcgatta catcagtaat attcgcccaa atgactgtat taaaaatacc taaccccata
     5641 tatccaacag aagtaagaac taagaataca tacatattac taggctttat caaaaataaa
     5701 agtaaatata caatagcaga gaagaacatc cctactgacg ccaattcttt tttaccgtat
     5761 tttttactga aaattaaact tacaggtgaa acaagtacca acatcaaaat aggattgata
     5821 aagttcatta aaactattcc ctgtgcatta ttataataat ctggaaatac ataattgttc
     5881 atactcattg ttaaaagctg agcaagcaac aaaaaaatag ctgcagctat tatagaaagc
     5941 aacgctctat ttttagctaa tccttttaaa gatttcaaca atgaaacttt taactgcgat
     6001 tcatcacttt taacccgttc tacagttaat ttataacata ttacgtaaca aataactgca
     6061 cacactgaaa atactcctgc cacaattgta aaagttgatc caccacgtac aatttgagct
     6121 ccagaactat ctcttgtata aataaaaatt ggtgttaaca cgccaatgaa catacctgct
     6181 aaggtagctc ctatactacg aaatgttgat aacgctgtcc tttcttctgc aacagaagtg
     6241 atagctgatg ccattgaacc gtaaggaata ttaatagatg tataaaaaat acttccccat
     6301 agtatatatg ttacatacat atatataact ttaatactca tagaagctga tgccattgat
     6361 gtttgataca ttaaaaaact tgctaaagcg actggaccac tcatccgtaa aatccacggt
     6421 ttaaatttgc cagttttaga tggttttgat ccatccacaa tacgccccat tgttatgtct
     6481 gtaactgcat caataaatct tgctactaaa aataatgttc ctacggtagc tccgcttatc
     6541 cccaatactt ttgtataaaa aatcattaaa aaagaactag caaaaatgaa agtgaaatca
     6601 ttcccaaaat ctccaaacat atatcctatt ttgtctctta gaccaaattt tctttccata
     6661 taatttgcgc tccttcctat tgttaagcgt tttcattctg ttgaactgcc aacttacgta
     6721 ctatactctg aagtataaag agttagtatt tattttttac attttatagt attatttatt
     6781 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctattcgag
     6841 ttacttcttc aaaatcacct ttttcaacag gagccaacaa attcccacct acaccaactg
     6901 caacaacgcc tgcttcaaac cactcttcta agttatctag acttactccg ccagtaggca
     6961 ttatatttac atatggtaat ggagcttgat acgtttttac aacagatgga ccaaaagcag
     7021 aacctggaaa tagtttaatg atttctactc cagcggtaag tgcctccttt atctcagtaa
     7081 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctct gctgtttgtc
     7141 gatcaaaaga tggacttacg atatattctg ctccagccat aattgcaata cgagcagtaa
     7201 acgcatctaa aactgtaccg gctccaatta ttacatcttt ttgatcagca tattttttag
     7261 caatttcttt gattatttct tcagcattcg gaactgtaaa agttagttca atccctttaa
     7321 ttccacccgc agtaattgca tttattgctt taatggcctc atctttattt tttccacgta
     7381 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
     7441 tctccttctt ttatggatac atactctagc tagttgtact aacttacaat aaataaattt
     7501 aagcttttat tttttgtatt attttctact ttcgatttca aaaaaattaa aataattata
     7561 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
     7621 tcgacctgct ttaacccact cgcttagtaa ttgacataat actcgacgga aatactcatg
     7681 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
     7741 actttgttgc gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
     7801 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
     7861 tgctagtgga agccaatcgg ttggatttaa tgaatataaa attgtttttg gtaagcaccc
     7921 actcttttca gcagtgtcga acaatgctac aacattttgt gtcaaactag cttgatcccc
     7981 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
     8041 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
     8101 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
     8161 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
     8221 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaataatcca ctcgttggct
     8281 caatgcctgc ttcaacccat taaagttttt gattggtata cccgctgctt gactaagttt
     8341 taaaatatag ttcgaaaatc ctgatgattt gatactccag accttatctg gacggaaagt
     8401 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatatc gcaagtcatc
     8461 tgtaggatca tccgttgtac aaatggcttt cacatccatc atcttcagca attcttttgg
     8521 gcgataggaa tccgtttgga ttttttcgtt ggcacgtcgc caaatttctg gtgcattctt
     8581 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
     8641 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
     8701 attacctgca ccactgatca actcttcttc tatcccatta gctcgcatca atcgccattt
     8761 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
     8821 attttctagc aaacatgctg ggtctaaatg acaatgataa tcgatgattg gttggtgttc
     8881 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
     8941 aaacatattt aagcactcct ttccttccct tagtttttag aagagaaaat ttttcttcta
     9001 aatagactcc attatataat tatttactcg cccccttttc aagcgcttcc caaataccat
     9061 ttaaataaac tgcacctaga gctcggtcat ataacccata accaggtcta ccggtttctc
     9121 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
     9181 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcataaa
     9241 aatcgagtcc attatcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
     9301 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
     9361 ttccagtaca taacgtaata ccattagaag gagaatccac tacattacac aaccattcta
     9421 aatcgtcacg atttttaaca atacgaggta atccgaaaat agaatatggt ggatcatctg
     9481 gatgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
     9541 atgcaaagtt ctctcgcaat ttcgcttcat caacttcttt ataggcttct aataattgct
     9601 tgatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
     9661 ctacttttct aataatttcc tctggatcag taggaatatt ttttgaaata taggctaacg
     9721 tagaagaccc atcatctaaa tgataatcca aatctgattt tacccaatca aagactggca
     9781 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
     9841 aattctcgat gtattgatct cgtgttggca aaccaatttt gatatcatca tgaacattaa
     9901 cactctcaat aactttcaag gtaaggccat tggcttcgac ttcatctttt aaagctttaa
     9961 ttcgttctct cggccagacc tctccggctg gtatatccat taatgttcca acaattcctt
    10021 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    10081 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    10141 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcaa ttttctttcc
    10201 tatctcaact tgatataaat ctaaaccaaa tatttcctta ttttctaaga tttcctgtac
    10261 cgcacaatga atctttgcag gatcttgttc acctaatttc aatactgaga atttttcctg
    10321 caactctacc aataaaggat caggacttgg cacaaaattt tttccttgat catcgactgc
    10381 caataaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    10441 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    10501 agtatctgtt gcaattcgtt gcggtgtatc tggaatatag ggattaggca aacgtttatt
    10561 gattacttca tcaataaatt gttttggatc aataacgcct ggactttcaa ctactggtaa
    10621 gccttctaca taacctatcc ctttaattaa gccaaccaaa tcatcatctt gcatttctgc
    10681 ctgaattgat ttatacccca acaaacaacc tgttattgct aatgccgtat ggagcggatt
    10741 taaacatgtc gtaaccttca tgatatctgc tttatcaact gtttctctat ctgtcataat
    10801 cacaccagcc gcttccagtt taggtcgacc atttggaaaa ctatcttcaa tgactagata
    10861 atggactttt tcagtattgg taaaagcagc gatattggta tttttcgcag tatgaattat
    10921 atctaagtct tcgaaacccg ccttcaataa atccattttg acactttccg aaggatttgg
    10981 cgtaattcta tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    11041 aataaatttt tcagaaacgt atcctttttt ttgccaagct tctgcaatca taaaaatgct
    11101 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggtaa
    11161 ctctccagca ttaaacctgc tatacaacaa ggatgtcaca atactcatcg tatgttgagg
    11221 attggtaggt ccagatttta tatctgcttc cactaccgaa aaaaattgtc ctttagaatc
    11281 ttttaaactg tatccttttt cagtaatagt aaacgttgta aactgtaatg taggatcttc
    11341 aaaatattta accactcttt gatagtccgt tatattcttt ggattgcaat ataaactatc
    11401 agctgttact gctaaaagtc tttttttgag ttcaccatct tcacccataa caatctccaa
    11461 aatatcattg ttgtagtcat gataagcttt ttctactact tcttcatcaa atgtctcaca
    11521 cacaactact ccggattcta attcattttg atctgctaag cattgagcaa tttctgcatg
    11581 aaatcctcga taaagatttc ctccaccaaa atgaagccac actggatgtt tctgaccttc
    11641 ttgtttgacc gcttcgacat taaaatacgg tagttgaact tgcattttgt tcaatccttc
    11701 gatagaatct tgccagtctt taatcaaaac catatttacc cctccatact gatatgttag
    11761 tttccgttac aattgtagta taaaatcgaa tgtaatcgct gtcaataaga aaaaactaaa
    11821 aatattcttt tttctaccat gtcagaatta ttgtgtcttt ttttagtaaa agcacaccaa
    11881 tatatatttt ttagaactaa cttaaattaa agcttaattt ctctttgatc ttatttgagt
    11941 attttttgtt tttttaatag ctagtatgtt actttgaaat ttgatattat ataacgagca
    12001 tagaactaaa atatcaaatt caaaaatgaa attttgagag ggattaaata tgacaattaa
    12061 aactagtctc caaaatcaag cttatcaatc tattcgtcag caaattatct acgctgactt
    12121 agaacctgga aaaaaagtat cagaaaaaaa attggaagaa acgctttcta tcggtcgtac
    12181 tccaatacgt gaagcattga ttcaactccg tcatcaagga cttgttgata caattcctca
    12241 aagtggaaat tatatctcat taatcgactt agatttagca aaaaatgctc gttttgtcag
    12301 agaacattta gaacgacaaa ttatgctaga gtgttgtgcc aagatgaata atcaaaagaa
    12361 acaagtttta gaaactataa ttgcagaaca agaaaaagct gttagtcaaa agaatgctcg
    12421 tagttttttt cataatgata atttatttca taaagcttgt tttgaaattg cagaacggga
    12481 agaagtatgg caatggttgg aagataataa tacgcacttg gaacgatttc gttggttacg
    12541 agtaaaaacg gaaggattaa aatgggaaaa aattatggaa caacatcatc aactatttaa
    12601 agccttgacc atgcaagacc ctgaagaagc taacttctta acatccttgc atcttcattt
    12661 aatgcttgat gaacaaaatg ttgtattgac tactcaccca aaatatttta aaaatatata
    12721 actgctaaat ttcttttttt taatttattt acatttctga ttactacaga aaactaacta
    12781 ctttttttat gtaaagttct gtcaaatata gtcactttat attactttct cttgagtaaa
    12841 aaaacatgtg aggtcgtaaa caaagattga actatctaca ttcat
//
LOCUS       DAPCMU010000078        12734 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPCMU010000078 DAPCMU010000000
VERSION     DAPCMU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.081"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="RD744_001777"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="RD744_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDU0557771.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="RD744_001778"
     CDS             complement(322..891)
                     /locus_tag="RD744_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDU0557772.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="RD744_001779"
     CDS             1077..1979
                     /locus_tag="RD744_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0557773.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="RD744_001780"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="RD744_001780"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDU0557774.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="RD744_001781"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="RD744_001781"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDU0557775.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="RD744_001782"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="RD744_001782"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDU0557776.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="RD744_001783"
     CDS             8547..8732
                     /locus_tag="RD744_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDU0557777.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="RD744_001784"
     CDS             complement(8794..9990)
                     /locus_tag="RD744_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDU0557778.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="RD744_001785"
     CDS             complement(10160..11596)
                     /locus_tag="RD744_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDU0557779.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="RD744_001786"
     CDS             11889..12698
                     /locus_tag="RD744_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0557780.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAPCMU010000079        12586 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPCMU010000079 DAPCMU010000000
VERSION     DAPCMU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.082"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            99..1982
                     /locus_tag="RD744_001787"
     CDS             99..1982
                     /locus_tag="RD744_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDU0557781.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2057..2341)
                     /locus_tag="RD744_001788"
     CDS             complement(2057..2341)
                     /locus_tag="RD744_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDU0557782.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2331..3065)
                     /locus_tag="RD744_001789"
     CDS             complement(2331..3065)
                     /locus_tag="RD744_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDU0557783.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3194..3817
                     /locus_tag="RD744_001790"
     CDS             3194..3817
                     /locus_tag="RD744_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDU0557784.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            4009..5130
                     /locus_tag="RD744_001791"
     CDS             4009..5130
                     /locus_tag="RD744_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDU0557785.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5137..8265
                     /locus_tag="RD744_001792"
     CDS             5137..8265
                     /locus_tag="RD744_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDU0557786.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8284..11478
                     /locus_tag="RD744_001793"
     CDS             8284..11478
                     /locus_tag="RD744_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDU0557787.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11624..12331)
                     /locus_tag="RD744_001794"
     CDS             complement(11624..12331)
                     /locus_tag="RD744_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0557788.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4290 a         2215 c         2652 g         3429 t
ORIGIN      
        1 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
       61 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
      121 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
      181 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
      241 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
      301 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
      361 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
      421 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
      481 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
      541 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
      601 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
      661 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
      721 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
      781 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
      841 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
      901 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
      961 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
     1021 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
     1081 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
     1141 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
     1201 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
     1261 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
     1321 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
     1381 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
     1441 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
     1501 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
     1561 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
     1621 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
     1681 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
     1741 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
     1801 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
     1861 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
     1921 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
     1981 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
     2041 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
     2101 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
     2161 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
     2221 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
     2281 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
     2341 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
     2401 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
     2461 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
     2521 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
     2581 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
     2641 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
     2701 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
     2761 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
     2821 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
     2881 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
     2941 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
     3001 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
     3061 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
     3121 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
     3181 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
     3241 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
     3301 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
     3361 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
     3421 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
     3481 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
     3541 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
     3601 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
     3661 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
     3721 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
     3781 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
     3841 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
     3901 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
     3961 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
     4021 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
     4081 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
     4141 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
     4201 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
     4261 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
     4321 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
     4381 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
     4441 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
     4501 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
     4561 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
     4621 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
     4681 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
     4741 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
     4801 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
     4861 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
     4921 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
     4981 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
     5041 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
     5101 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
     5161 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
     5221 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
     5281 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
     5341 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
     5401 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
     5461 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
     5521 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
     5581 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
     5641 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
     5701 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
     5761 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
     5821 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
     5881 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
     5941 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
     6001 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
     6061 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
     6121 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
     6181 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
     6241 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
     6301 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
     6361 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
     6421 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
     6481 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
     6541 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
     6601 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
     6661 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
     6721 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
     6781 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
     6841 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
     6901 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
     6961 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
     7021 agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
     7081 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
     7141 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
     7201 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
     7261 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
     7321 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
     7381 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
     7441 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
     7501 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
     7561 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
     7621 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
     7681 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
     7741 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
     7801 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
     7861 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
     7921 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
     7981 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
     8041 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
     8101 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
     8161 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
     8221 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
     8281 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
     8341 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
     8401 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
     8461 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
     8521 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
     8581 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
     8641 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
     8701 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
     8761 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
     8821 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
     8881 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
     8941 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
     9001 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
     9061 caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa
     9121 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
     9181 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
     9241 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
     9301 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
     9361 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
     9421 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
     9481 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
     9541 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
     9601 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
     9661 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
     9721 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
     9781 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
     9841 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
     9901 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
     9961 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    10021 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    10081 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    10141 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    10201 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    10261 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    10321 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    10381 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    10441 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    10501 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    10561 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    10621 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    10681 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    10741 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    10801 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    10861 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    10921 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    10981 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    11041 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    11101 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    11161 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    11221 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    11281 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    11341 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    11401 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    11461 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    11521 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    11581 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    11641 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    11701 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    11761 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    11821 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    11881 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    11941 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    12001 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    12061 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    12121 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    12181 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    12241 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    12301 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    12361 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    12421 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    12481 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    12541 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcga
//
LOCUS       DAPCMU010000080        12465 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPCMU010000080 DAPCMU010000000
VERSION     DAPCMU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.083"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(74..436)
                     /locus_tag="RD744_001795"
     CDS             complement(74..436)
                     /locus_tag="RD744_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557789.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(510..890)
                     /locus_tag="RD744_001796"
     CDS             complement(510..890)
                     /locus_tag="RD744_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDU0557790.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            954..2147
                     /locus_tag="RD744_001797"
     CDS             954..2147
                     /locus_tag="RD744_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0557791.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2224..2808)
                     /locus_tag="RD744_001798"
     CDS             complement(2224..2808)
                     /locus_tag="RD744_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDU0557792.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3009..4007
                     /locus_tag="RD744_001799"
     CDS             3009..4007
                     /locus_tag="RD744_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0557793.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4119..4628
                     /locus_tag="RD744_001800"
     CDS             4119..4628
                     /locus_tag="RD744_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDU0557794.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4658..5317)
                     /locus_tag="RD744_001801"
     CDS             complement(4658..5317)
                     /locus_tag="RD744_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDU0557795.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5319..6083)
                     /locus_tag="RD744_001802"
     CDS             complement(5319..6083)
                     /locus_tag="RD744_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDU0557796.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6091..6768)
                     /locus_tag="RD744_001803"
     CDS             complement(6091..6768)
                     /locus_tag="RD744_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDU0557797.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6805..8190)
                     /locus_tag="RD744_001804"
     CDS             complement(6805..8190)
                     /locus_tag="RD744_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDU0557798.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8400..8484)
                     /gene="ffs"
                     /locus_tag="RD744_001805"
     ncRNA           complement(8400..8484)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RD744_001805"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8507..9022)
                     /gene="tadA"
                     /locus_tag="RD744_001806"
     CDS             complement(8507..9022)
                     /gene="tadA"
                     /locus_tag="RD744_001806"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDU0557799.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9129..9986
                     /locus_tag="RD744_001807"
     CDS             9129..9986
                     /locus_tag="RD744_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557800.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10067..10402)
                     /locus_tag="RD744_001808"
     CDS             complement(10067..10402)
                     /locus_tag="RD744_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557801.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10469..11839)
                     /locus_tag="RD744_001809"
     CDS             complement(10469..11839)
                     /locus_tag="RD744_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDU0557802.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11917..12453)
                     /locus_tag="RD744_001810"
     CDS             complement(11917..12453)
                     /locus_tag="RD744_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0557803.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3692 a         2541 c         2098 g         4134 t
ORIGIN      
        1 tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg
       61 cttaatctgt attttagaag aaaggcactc cctctacttc gtaagttcca agaacagagc
      121 gcatttgacc tggttcttcc caaatatatc cttcgcctaa ttcttctctt tgattctttt
      181 gttcagtttc aaagacaaac aacggaacat tttcctcttt aatatgtgta tttgtcaatt
      241 ccatcaaact aatcttgcta acatcgatat gaactatttc ttttaagaaa ttaagtatat
      301 tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa
      361 ttgaatggac caaaaatttt ttagtaccat cctccaaatg caacataatt gtaccagcaa
      421 ctcttttgct tgccaaaatt tccacctcga tcaacttgtt ctatggctta cataaaaatt
      481 ctagcacatt ttcagaaaaa atacactttt tatttcttat tttctttccg ttctagttca
      541 gataatgctt cttctatcca aataggtaac tgctccgcaa tagtggtaaa tccgatctta
      601 cgatttttat tcgtgaaata cctttttctg cgatatggaa cagctggatt tgttttttct
      661 aacgttctta aagataaact gatttttttg gaatattcat caatgtcgat gatctgaact
      721 gttactggct gtccgacttt taacaatgaa tggatatttt tcgtataacc ggattgtact
      781 tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc
      841 tgtatccctg tcacttttcc ttcaattatt tgtccaattt tataagtcat ctacttccct
      901 ctcttcgcat ttatcattat actgtaaata gctcaaaaaa agaagcgacg aaaatgactg
      961 gaggtcttaa gatgatcgaa tttgataaac agatttcaag aaaagataca gaaagtgtaa
     1021 aatgggacgc aattgctgag acatatcaaa tggatgactt acttcctttg tgggtagcag
     1081 atatggactt cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg
     1141 gaatctttgg ttatgccact ctgtctgata aactatatca ggctatcatt cattgggaac
     1201 gccaacacca tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa
     1261 ccagtttagc tgcagcagta caaactttta caaaaccagg cgattctatt ttgattcatg
     1321 acccagttta tcctccattt tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa
     1381 gctctttgct agaaaaaaat aatcatttca tcatggactt gtctgacatg gagaaaaaaa
     1441 tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg
     1501 tttggagcaa agaagaattg aaacagttaa gcgaattgtg tttaaaacat caagtgattc
     1561 tgtttagtga cgagatccat caagatttaa ctttattcga tcatacattt acttccatgc
     1621 taacgattga tcctaattta gatgatctac tagttgcttt tacttccgca accaagactt
     1681 tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa aaatccagag atgaaagctg
     1741 attttgaaaa acatttagtg atgaaccaac agcacgaaat caacactttt ggactgattg
     1801 gaacccaagc ggcttatgag acgggtggag aatggttaag ccaactgatt ccttatctag
     1861 aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa gtgagaataa
     1921 tgaaccctga aggaacttac ttaatgtggt tagattttac cgcctatatt tcagacgacc
     1981 aagaattgga gaacactctt gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt
     2041 ttggtccttc cggtcatggg catatgcgct tgaatattgc ttgtccagaa gaaactttac
     2101 tggaaggact taatcgaatc aagtatgctt tagagacaat cgattaaatc atttattcaa
     2161 aataaaagga cgagacctta ctaggtttcg tcctttttca taattactcc gtaccatacc
     2221 aaactattct gaaatttcaa ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg
     2281 accacgttgt acatttgcga tttcatctac tgtgtccatc ccttctagta catgtccaaa
     2341 gactgtatgt cggaaatcta gccaaggagt tccgccacct ttatacgctt caatgatttc
     2401 tgatgggaat ccagcacctt ctagttgtcc aagcatatta gctggcacat ttgtgttatt
     2461 tacaataaag aattggcttc cgtttgtgtt tggacctgaa tttgccatag ataaagctcc
     2521 gcgaaggttg aacagttcac gactgaattc atcttcaaaa ctttcgccat aaatactttc
     2581 gccacccatt cctgttccag ttggatcgcc gccttggatc atgaaatcag gaataacgcg
     2641 gtgaaaaatc acgccatcat aataaccttt tttggaaagt tcaatgaaat tttgaaccgt
     2701 cttcggtgct agttcaggga ataattgaac agtaatgtca ccacggtttg ttttgataac
     2761 agcttttggt cctttttcac tttttaaatc tagttgtggg aatgccatat tgtttcctcc
     2821 taatatttgc atacttcata ataataataa cataatacct aacctctcgt aaaaatacta
     2881 gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc
     2941 gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag
     3001 gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg
     3061 ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac
     3121 ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc
     3181 cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc
     3241 atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga
     3301 ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt
     3361 cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg
     3421 attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg
     3481 gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga
     3541 ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat
     3601 ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga
     3661 cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc
     3721 tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata
     3781 gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg
     3841 cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg
     3901 aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc
     3961 ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc
     4021 tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca
     4081 tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt
     4141 tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac
     4201 aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg
     4261 aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc
     4321 aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag
     4381 atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg
     4441 gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag
     4501 aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga
     4561 tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta
     4621 ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttcttttttg
     4681 tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc aaaaaataat tctaataata
     4741 caaaaaataa gataaagcta tgcatgaaaa attcttctgg aagtacattg atgattggat
     4801 cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa
     4861 aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac aactggcagt aacatgccga
     4921 tttgaaacgg acgaatcaac cgccaaagcc gatcattctt ttgcaaatat ttgataaaca
     4981 aaatactcgg gatcaaagtg atgaaaaaca cagcataatc cactaaaaac aaaattttca
     5041 cttcataaaa gtgatgggct ccgctagcag aaactgggaa atctggcaag ctgaggatgg
     5101 ttttgaaggg attattgaga taattcatta ggtgatcaaa atttttcagt aacgtctctt
     5161 gatctaaaga agtataatcc aaaatttgca aatgtccgat atcaaatacg tatagtggtc
     5221 gaaagttgat cgtcacacca actgccagac tgatcagtgt cagaaatagg ctgaacaatc
     5281 cagcatattc catccactgc catctcgaat ttttcatatt agaagctcca ttcatccagt
     5341 gattgtttca cataggtagc tgggataggc aacccaggta catcttcttc ttttgtaaaa
     5401 cctgataaaa cgagcaaagt atctatatta ttgcgtatcc cagcttggat atctgtttca
     5461 tagttatctc caaccatgat tacttcttct ttttgcagcc ctaatacttc aacggcttta
     5521 tccataatga tggcttctgg ttttccgatg tagatcggcg gcgtttgggt tgctgtctct
     5581 acaaaagaga tgactgaccc tgctccagga agaaggccgc gttcagtagg tatattctta
     5641 tctgggttcg ttccaatgaa agtagcacct ttttgaatag caagtgttgc tttaactact
     5701 ttttcatagg tcagataatt atctaagcct accactacat agtcaggagc ctcttcttcc
     5761 catgtaaatc cggcagctaa aatcaagtca atcaatcctg cttctccgat aacatagact
     5821 tttttccctt ttccatctgc tttcataaaa tcgattgttg ccaaagtagc tgtgtagact
     5881 gtctctggag ctacatgaat gtcaaactca tctgctagac gattagctac tgtttctggt
     5941 gacttagtcg tgttattggt aacaaacaag aaaggtaatt ttcttttttg caattcttcc
     6001 acaaatcgtt taccggccgg tatgggttgt gtcccacgat aaatcgttcc gtctaaatca
     6061 attaaatatc ctttatagtc cattcatcca ctactcttcc ctttttctga tcgtaaatgc
     6121 tgtttttgtt ttttctactt gtttaggttg ttcttgtttt tttacttttt cagaggtttt
     6181 tgctgactct gtttttcgat ttttaataac cggttttttg cgattagatg cgactggtcc
     6241 ttttttctca tcgatatacg cacgattatg gttttttttc ttttttcggc gctgagttgt
     6301 cttttctttt ttaccgccga ttctctcgat gacaaaatac gcacaaccaa aattgcaata
     6361 ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct ggatgcgccc gtcgattatc
     6421 cgcttggaaa aatcctttta ggcgtagttg ttcataaccc caatctccta cgatataatc
     6481 atatcgggct agtacatcac tgtaacgttc tccaagcttt tctgcattaa atccttcacg
     6541 ataatcggac acaagccgat aatgtctttc acctatcaaa aaattactgt catctaacag
     6601 tgtcactagc tcgcctttga ctttttctgc tttaggctcc tcaacgattt cttccaaagc
     6661 agctgtcagt tcttcggtga ctgttgtttc ctgttcagct gatgattttt tcttagcaat
     6721 ttctgtttta tgctcaacgt tctgctgttc attttgtttt gcagacatgc gttcaccacc
     6781 tatctttttt attatacctt gtttttattt gattgggtag tgagctttca agtaatctcc
     6841 taccacactt cggataaatt cagggaacaa aaattcattt tttccagcaa tctcaatcgt
     6901 tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta aaagtataat ttttattcag
     6961 atcgattggc ttgttcttcc aaaagacttg atggttaata gaatcatatg tgattccatt
     7021 atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag gatagttacg
     7081 taaaaagtta cgattttttt ctatttctaa aactaagcgg atcagatcat ttccacccaa
     7141 tgtgacattt aacaagtgca tcggatgcgg caacgcagtg tgaagctgat cctgattgac
     7201 ccgccctttt ggaatttcag ttaaaaacag accactgttt aatattgctg cttccgtctt
     7261 tcctcgttct ttcaccgcct caaaggctac ttggataaac gaatgctcat tgaagatatc
     7321 tagactcaga gaaaatggca aatctgctac ttctttttct tttaataatt catgcccacg
     7381 ttctagataa ctatttattt cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg
     7441 gatggctttg gctgaatgct taaggatttt tttctcttca tcgatggtta aatggacttc
     7501 accgacatac tgcccgtact ttccagcggc tgctatttgg acatcattga ttacgtgacc
     7561 ttctctgaat aaatgatggg tgtgcgatcc taaaataaca tcaattgctg gtatttcttt
     7621 tgcaatttgg aaatcatcct gaatcccgag atgactcatc aaaactaaaa catccacttg
     7681 tgaacgtaaa tcttcgacta attctggcaa aatatcataa ggattccgta tgtcccagcc
     7741 attcggatta taagtaagcg gaaatggagc agtaaatgca attagtccga ttttcgtctg
     7801 gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc ggtggctgga gcgtattttt
     7861 atcgaataag ttatccagga tgatatcaaa ctcagcatga tcgtataaat gattcaactc
     7921 ttctttggaa tttcctacgc cttcattgtt tcctatcgtc gctgcatcat aaccaacttc
     7981 attcatcagc aagatatttt cctgtccatt cgttgcttct gacaatggat gccaacgatc
     8041 cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg
     8101 tttacgttga tccaaaaacc ttctgatcct tggccaattt tctaaatgag aatgcagatc
     8161 attcgtatgt aaaatcacta ttttttccat gtaccctctc gcctctcttt atctttatca
     8221 taccataggc agctttttct tccattgaaa caagctctat ttcagagttt ttcctgcctt
     8281 gaagcaaatt gtataagtac aatattcgat aaatcagtct ggctaaaaaa tcaggaacat
     8341 tcatttttac agtactataa aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat
     8401 tataatcaga cacatggcgg aacctgctta gtgctgcttc cttccagacc tgacacgctt
     8461 cacaagccca ccattgtcct agcctttcgg caaagaatag tataccttat ttttcattcg
     8521 ttgaccagtc tttttttaat ttcttttttc gctggcggag ctttcggaaa aagtcagtca
     8581 aaatcatccc acattcgtct tctaaaatgc caccttctac atatgcttgg tggttaaacc
     8641 gctcgtcctc caaaagattc atcaaggtac cggctgttcc gccttttgga tcatacgcgc
     8701 cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct
     8761 ctaaggtaac aaaaagtgca gcatcttcca agcgccagct tcccactttt tcacaggctt
     8821 ccttgattgc aagcatttct gcatgtgtag tagcatcttg cgtggtttct ctaaggttat
     8881 atcctcttcc aatcactttt ccatccaata caaccactgc tccaatcgga acttcatgaa
     8941 gcatttctgc tttctttgct tctgccaatg caaggcgcat ccatttttct ttttctactg
     9001 catcaagatt tgtatttacc aattaccctt ccctgccttt gttttttctt gttttacttt
     9061 agcatatgat aaaaaggctg taactaactt ttttttctat ggtaaaataa atacaagagg
     9121 tgaacaagat gaaaatggat aaattacttg ctctttaccc acaagcacag aaaaaaaact
     9181 ctccagcatc agaagcggat atcctttctt ttgcaattga tgatgctttt atctggatca
     9241 aacaagattc attatctcaa caagaaacca gccttttgaa agcgttattc cctgtaatca
     9301 acgatcaaaa aaaacaccca tggtatcgct atctttttaa agatgcttct tgtgttcaag
     9361 aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag
     9421 aatggcaaga tactattttg gaattatttc gctattctgc tgatttcttt ttttatacag
     9481 acacagatgc gctcttagtc gaacaaataa accgtgatta tcttgatgct actgaaatca
     9541 acggtatttt tctatcttta gatgctgatt ttgatacgac aacgtctgta ttcgttggtt
     9601 ctttccattc acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt
     9661 taacagagca aaacaatctt tatactcaca gccgtacttt ttctatgcaa gatgtggctt
     9721 tacattacta tacaaaagat actgtaaaag atagtaagtt gatcaaaagt tacatagaac
     9781 tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg
     9841 tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa
     9901 aatttcaaga acagtctggt ttcaatctaa aaaaagccaa cgatttactg ttttgctatc
     9961 ttttattgct tcatgaacat aattaaagat agattaaagg ggttgtgaca ataattttgt
    10021 cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt
    10081 caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat
    10141 aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca
    10201 agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt
    10261 catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt
    10321 ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg
    10381 ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat
    10441 agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt
    10501 tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca
    10561 aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt
    10621 ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg
    10681 catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga
    10741 cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca
    10801 ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt
    10861 cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct
    10921 gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag
    10981 gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac
    11041 taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc
    11101 taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt
    11161 tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata
    11221 gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc
    11281 ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt
    11341 ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg
    11401 ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca
    11461 tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt
    11521 caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt
    11581 catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc
    11641 tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg
    11701 gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa
    11761 aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg
    11821 ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa
    11881 aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat
    11941 attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag
    12001 aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc
    12061 aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt
    12121 aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat
    12181 agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc
    12241 taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat
    12301 tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct
    12361 tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga acagcaaata
    12421 ttctttacta taatttgtta ctctccccat cattcttacc tccgt
//
LOCUS       DAPCMU010000081        12185 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPCMU010000081 DAPCMU010000000
VERSION     DAPCMU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.084"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(147..320)
                     /locus_tag="RD744_001811"
     CDS             complement(147..320)
                     /locus_tag="RD744_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557804.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            606..1934
                     /locus_tag="RD744_001812"
     CDS             606..1934
                     /locus_tag="RD744_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDU0557805.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2136..2969
                     /locus_tag="RD744_001813"
     CDS             2136..2969
                     /locus_tag="RD744_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDU0557806.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2985..3722
                     /locus_tag="RD744_001814"
     CDS             2985..3722
                     /locus_tag="RD744_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDU0557807.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3877..4845)
                     /locus_tag="RD744_001815"
     CDS             complement(3877..4845)
                     /locus_tag="RD744_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDU0557808.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5077..5910
                     /locus_tag="RD744_001816"
     CDS             5077..5910
                     /locus_tag="RD744_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDU0557809.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5944..6783
                     /locus_tag="RD744_001817"
     CDS             5944..6783
                     /locus_tag="RD744_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0557810.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6809..7369
                     /locus_tag="RD744_001818"
     CDS             6809..7369
                     /locus_tag="RD744_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0557811.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7568..9289
                     /locus_tag="RD744_001819"
     CDS             7568..9289
                     /locus_tag="RD744_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDU0557812.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9454..10596
                     /locus_tag="RD744_001820"
     CDS             9454..10596
                     /locus_tag="RD744_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDU0557813.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10612..11823
                     /gene="thiI"
                     /locus_tag="RD744_001821"
     CDS             10612..11823
                     /gene="thiI"
                     /locus_tag="RD744_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDU0557814.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2045 c         2531 g         3457 t
ORIGIN      
        1 ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt
       61 tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac
      121 gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa
      181 taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc
      241 ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc
      301 aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt
      361 attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc
      421 attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac
      481 atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga
      541 gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg
      601 attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa
      661 atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg
      721 aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga
      781 aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc
      841 gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt
      901 ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa
      961 aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg
     1021 gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga
     1081 tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac
     1141 tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta
     1201 ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag
     1261 gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg
     1321 accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt
     1381 atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag
     1441 agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa
     1501 ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg
     1561 atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg
     1621 atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa
     1681 gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa
     1741 aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga
     1801 gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga
     1861 aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg
     1921 gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg
     1981 cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta
     2041 taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac
     2101 gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg
     2161 atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc
     2221 gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat
     2281 tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata
     2341 cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg
     2401 gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc
     2461 cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg
     2521 aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag
     2581 aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg
     2641 tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga
     2701 gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt
     2761 atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat
     2821 ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga
     2881 tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc
     2941 aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata
     3001 ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt
     3061 cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca
     3121 gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta
     3181 atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata
     3241 agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa
     3301 tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag
     3361 agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg
     3421 aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta
     3481 atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact
     3541 ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg
     3601 ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac
     3661 atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat
     3721 aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat
     3781 tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag
     3841 cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa
     3901 ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact
     3961 attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata
     4021 atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt
     4081 ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa
     4141 agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg
     4201 catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg
     4261 ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg
     4321 acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg
     4381 tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca
     4441 acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact
     4501 tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa
     4561 aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga
     4621 tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg
     4681 tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc
     4741 tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt
     4801 catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt
     4861 acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat
     4921 cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa
     4981 aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa
     5041 agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg
     5101 tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg
     5161 tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt
     5221 gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga
     5281 atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat
     5341 tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa
     5401 ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag
     5461 tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa
     5521 tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat
     5581 gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt
     5641 gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg
     5701 cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa
     5761 gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt
     5821 ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga
     5881 ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag
     5941 tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa
     6001 gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt
     6061 gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat
     6121 atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac
     6181 ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa
     6241 atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg
     6301 atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa
     6361 aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata
     6421 ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta
     6481 gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc
     6541 ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa
     6601 atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta
     6661 ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg
     6721 actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat
     6781 tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga
     6841 aacattccac aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa
     6901 tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac
     6961 ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa
     7021 agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt
     7081 agatgcactg acagatatcc tttactttac ttatggttct ttttcattac taggaatagt
     7141 gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg
     7201 aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga
     7261 atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc
     7321 taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca
     7381 ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg
     7441 catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt
     7501 ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga
     7561 accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat
     7621 tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt
     7681 agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa
     7741 aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa
     7801 tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct
     7861 caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat
     7921 ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac
     7981 ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga
     8041 aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat
     8101 caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt
     8161 tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat
     8221 gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga
     8281 aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct
     8341 tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa
     8401 aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt
     8461 atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac
     8521 gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga
     8581 tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca
     8641 aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca
     8701 cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga
     8761 tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa
     8821 agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt
     8881 agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac
     8941 tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt
     9001 caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga
     9061 tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca
     9121 tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata
     9181 tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc
     9241 tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt
     9301 tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt
     9361 gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata
     9421 atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca
     9481 accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg
     9541 aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga
     9601 aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga
     9661 acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat
     9721 catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa
     9781 gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac
     9841 gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat
     9901 gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa
     9961 attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg
    10021 acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc acggggaact
    10081 ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa
    10141 gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct
    10201 ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta
    10261 gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac
    10321 tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat
    10381 gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat
    10441 atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt
    10501 cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc
    10561 aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac
    10621 agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt
    10681 atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc
    10741 catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt
    10801 ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa
    10861 gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa
    10921 gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa
    10981 ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg
    11041 cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat
    11101 gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg
    11161 ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa
    11221 attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca
    11281 aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca
    11341 tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt
    11401 actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc
    11461 attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct
    11521 atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa
    11581 atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat
    11641 tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat
    11701 caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag
    11761 atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta
    11821 taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag
    11881 ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta
    11941 tcaagaagtt atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag
    12001 agatcaagct acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt
    12061 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa
    12121 tttttgtgaa atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga
    12181 ggttg
//
LOCUS       DAPCMU010000082        11981 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPCMU010000082 DAPCMU010000000
VERSION     DAPCMU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.085"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(221..1423)
                     /locus_tag="RD744_001822"
     CDS             complement(221..1423)
                     /locus_tag="RD744_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0557815.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="RD744_001823"
     CDS             complement(1426..1620)
                     /locus_tag="RD744_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0557816.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="RD744_001824"
     CDS             complement(1667..2515)
                     /locus_tag="RD744_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDU0557817.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="RD744_001825"
     CDS             complement(2720..3070)
                     /locus_tag="RD744_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557818.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="RD744_001826"
     CDS             3665..4111
                     /locus_tag="RD744_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDU0557819.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="RD744_001827"
     CDS             4735..5727
                     /locus_tag="RD744_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0557820.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="RD744_001828"
     CDS             5743..6627
                     /locus_tag="RD744_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0557821.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="RD744_001829"
     CDS             6676..8283
                     /locus_tag="RD744_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0557822.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="RD744_001830"
     CDS             8284..9768
                     /locus_tag="RD744_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDU0557823.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="RD744_001831"
     CDS             9945..10937
                     /locus_tag="RD744_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0557824.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="RD744_001832"
     CDS             10989..11858
                     /locus_tag="RD744_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0557825.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3710 a         1960 c         2484 g         3827 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca a
//
LOCUS       DAPCMU010000083        11956 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPCMU010000083 DAPCMU010000000
VERSION     DAPCMU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.086"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(115..1578)
                     /locus_tag="RD744_001833"
     CDS             complement(115..1578)
                     /locus_tag="RD744_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDU0557826.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1642..4659)
                     /locus_tag="RD744_001834"
     CDS             complement(1642..4659)
                     /locus_tag="RD744_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0557827.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4810..5649)
                     /locus_tag="RD744_001835"
     CDS             complement(4810..5649)
                     /locus_tag="RD744_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0557828.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5794..6039
                     /locus_tag="RD744_001836"
     CDS             5794..6039
                     /locus_tag="RD744_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557829.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6102..6524
                     /locus_tag="RD744_001837"
     CDS             6102..6524
                     /locus_tag="RD744_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557830.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6618..6935)
                     /locus_tag="RD744_001838"
     CDS             complement(6618..6935)
                     /locus_tag="RD744_001838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557831.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6962..7168)
                     /locus_tag="RD744_001839"
     CDS             complement(6962..7168)
                     /locus_tag="RD744_001839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557832.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7149..7331)
                     /locus_tag="RD744_001840"
     CDS             complement(7149..7331)
                     /locus_tag="RD744_001840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557833.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7345..7695)
                     /locus_tag="RD744_001841"
     CDS             complement(7345..7695)
                     /locus_tag="RD744_001841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557834.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7721..8023)
                     /locus_tag="RD744_001842"
     CDS             complement(7721..8023)
                     /locus_tag="RD744_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557835.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8026..8259)
                     /locus_tag="RD744_001843"
     CDS             complement(8026..8259)
                     /locus_tag="RD744_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557836.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9245..9567)
                     /locus_tag="RD744_001844"
                     /pseudo
     CDS             complement(9245..9567)
                     /locus_tag="RD744_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9690..10805)
                     /locus_tag="RD744_001845"
     CDS             complement(9690..10805)
                     /locus_tag="RD744_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDU0557837.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10811..11209)
                     /locus_tag="RD744_001846"
     CDS             complement(10811..11209)
                     /locus_tag="RD744_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDU0557838.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         3194 a         2434 c         1833 g         4495 t
ORIGIN      
        1 agatagatac gagatttcaa attaaaaaac gaaaatgaga gatacaaata tatcaaatat
       61 ttaaaaagta accacatttt ctataccact ccttatcaga aacagcctta ttctttagaa
      121 taagactgtt tctgggttga ttaatttgtc ttttgttaaa ctaccatcta aggcaagttc
      181 taaatgtaaa tgtggtcctg tactatttcc tgttgaaccc acataaccaa taatttgtcc
      241 ttgttcaact gtttgtccta cgttcacgca atattcctgt tggtgcgcat aaagagccac
      301 acttccgtct tcatgctcaa ttgtgacata atttcctgac aaagtatgat acgaagcttc
      361 tatgaccgtt cctgccttgc tcgcatagat tggttctcct tgattggctg ccatgtctaa
      421 tcctctatga aattcttctc ctcgccaacc gaaggaacta gaaattgaat aattttttag
      481 aggtaccata tagccttctt tgcttagttg tggaccagct acttctttat catatttgat
      541 taagtcatag gtttcgatta acccatttaa ttttttatcg tagctagtgt ctgttgcgta
      601 tcttcctgtt aaaaatttag tcgcttcctg ataggttttt gccttacttt tccaaacacc
      661 atcataaaaa gaggtatttc caactaagcc ttcttttaac agtttggcat agtcttttaa
      721 gctttcttca tagctctcat attgccgaaa agttgcttgg gtcgtataaa gtgtcccatt
      781 gcctaaatct tcttgtgtcg caaaagagac gccctttccg ttatgtgtgc ctttgattcc
      841 aaatagattg tagtttggtg cctgagccaa ttgactttgt ccgcttgcgg attctaaaat
      901 ggcttgcgcg atcataacag aagcgtataa atcattttct tgtccaattt tacgagcaga
      961 ttcaccgatt ttacggataa acgtttctac agacgcgtct ttttcaaaat gaatagtccc
     1021 ttcgttatct tgtgtgctgg aagggactaa ttcttgtatc ggtgcgcttg gtgcttggtg
     1081 gtagtttttt gtctcttctg aaccgattga ttgtgcctgc ggtacgtcat tcataggtac
     1141 agtcacactc tgttcaggtg ccttttgtgg gcgttccgtt tttgttggcg gttccgcttc
     1201 tgtttcggta cttggttttt gaggagtctg tgagctttca tcagaaggtt gctcatttgg
     1261 ttcttctgac ggttcctcct caggttgttg attgtcttct ggtttgtctg gttgattcgg
     1321 tgtttctggt tgttcttcag aacctgttgg tggagcgttc atttctggat tatctggtgt
     1381 atctattgta tcttcagtcg gtgtttctgg ttgattttct tctccagtgg aatttgttgt
     1441 cgtttcttca gtgatcgttg cagattgcga atttgtcagt tgattgttgc cttgttcatt
     1501 agccaaaacc gctggcgtat acccaaatac ttgacctact aataacaaag cagataaaca
     1561 tttactcgtt tttttcacaa gattgcctcc attcttcaat caatcatcta gagacagctg
     1621 attgctgtct ctagtcatac tttaattgct ttgtttcatt ttacgacgaa ccagaacagc
     1681 ccccacacca acaaaaagaa acagtcccaa tgcaattaac acatcgttca ctgtttctcc
     1741 tgtttttggt aagctttgtt ttggtgtgct cgtcggcgtt tgtttgacag gggttgtcgg
     1801 cgtttctttt ttaatgcctt ttgctgtcaa tgtttgtttt tcattcttca aatcaaagtg
     1861 acgtgcttct tcctctttat ggtctttatc cttatagaca atatgagttg cgaccaattt
     1921 ctccccgtct tttaacgtgc ctgctttgta gtcaaaggat actttaaact cttcagacgc
     1981 tttagaaact ttacgtgcag tttctaccgt accaacgact gtatcttttc catttttatc
     2041 aatcttatgg aattgagtca cccagtattt ggtttcccct accgtagatt ctggaaattc
     2101 ttgcgataca tgatcgatca actcttggtc tttgtctgga tcaaatgttt ggtcgccatt
     2161 cactgtaata aagcgtgttt cgatttttgg ttctttcgca gtcactgttt gatctttatt
     2221 ggttaaatca aaatgacgag cgaattcttc ttcctcttct ttgtcgttat agacaatgtg
     2281 cgttgcgaca atcgtgtcac cgtctgaaac catgtctgct ttgtagttga acgtcacatc
     2341 aaatgtttct ttttcatcgg tcacttttcg tgtttctttt accgtatcta atacagtgac
     2401 ttttccatcg gcagtaattt tatggaattg cgtcacccaa tatttctctg tcccaaccgc
     2461 agatttaggg aatacttggg acactttatc cactaattct tggtcttttt tcgggttgaa
     2521 atcttttcca cctgtcgttg tcacaaacaa agtagaaatt tctggttggt cttcttctgg
     2581 aaccacgatt tcttcgtttt tcacagtaat tggttgtact tctccgtctt tgaccgttac
     2641 taaaatcgtt tctgtttgtc ctgacgcttg cccataatgc tcagggaaca caatttccgt
     2701 aaatttatac gtaccgtctg gtaattttag tttttctggt tcttcttttg tttcatacac
     2761 aaaggacaca tttttattat gtgcagtcat accttcaatc aggaatttcg ctcctggtaa
     2821 ttgtagatga ttgattgaat cgactttatt gaagtctact tcacccgttt ttaacaagtt
     2881 gtcaatcaca tctaattcaa acgtaagcac aggcgtcgtg ttatcgccat aggccacaga
     2941 gaacgtggtt tgttcttcag aaggttgacg gtagttgtct ggcacatttt tttcaatcaa
     3001 ttggtatttg ttttgatcgc cattgtagaa ttttttgttt gccttccagt gtccgtcttt
     3061 gtcagttttt actgaatcca agacttctcc ttttttcaca aggactgttt ctttatctgg
     3121 cgcagtaata tctgttttgg cgataatatc aaattcgata ttttctgcag gaacggtatc
     3181 ataaacaggt ttgccttgat tccaggattc ttccgcatta tagacttctt tgactttctt
     3241 tccttctgga atgaactgtt gtaagtcatt ttttgcagtt aacgtcactt ctttgtttgg
     3301 ttcaatcgta atcgtatgag tttgtttgtc tagaatatac ccagctggtg ctttgacttc
     3361 tttgacatcc aatttatttt gaacatattt tccgtctttt tcatagaatg cagggatttc
     3421 ttttaaggcg atttcccctt tggcatccgt tgtatattgt tttgtttctc ccgtatcatg
     3481 acgtttgatt tcatacgtca catttggaac ttttttccct gtgacagcgt ctagtttggt
     3541 tagtttgaag tcgccatttt ttagaacttt aatagttagt ccagctctat taggatctgg
     3601 cacataaagc tttcctagat tttgagcaac aggatctaca taggcaaaac ttgaacctac
     3661 ccctctagaa tttggatcca gtgaagaaac agaaattcgt ccgtcatttg agttttgatt
     3721 tgcttttata aacaaactat ttcctgattt tcttacttga acgttagctg tgttttcaac
     3781 aaccgaccca tcccattgag aaaacacacc ttttgtatcc ttaacttcta cttcttcccc
     3841 tactttaacc gtcactgttt ggctatcaaa agaaggatct tcaagtaaat tgtttatttt
     3901 tctatctaca gtctgtttga aagcttcata attagattgt ccaatagttc cataattaac
     3961 aaatgaacca cctaaaactt cccaaatata tgtttgtgct aatacatagt tatccattga
     4021 accactagaa tagtagccaa aataaccaat taaagaaatc ttttttctaa tagcacttgt
     4081 cgtaattaaa ttttcgagtg gtgtcacatc atattgaata cccgcactcg tgattgcgcc
     4141 ttcttgaatg caaaaaacat cttctccgtc aatttgccag cgaaccatgt aatcatgagt
     4201 atacattgtt ccttcaggag taattcgagt catattcaca tctttacgac cattttcagt
     4261 caatgttaga ttggtagcac gtgtctgtgc attcttttcc aagattgctt gccattcttc
     4321 agattcttgc gccaatcgtt cttgttcaga aaaagaagtc gctttttgaa tatcttcaat
     4381 ggttataaag tatttctcat aaccttcagg gacctcttga gtcattacat tgggcggaga
     4441 aacatcttct gtcgtgtcct gcgtagttgt ctcttctgtt gtttcacttg tggtttcagt
     4501 actgatcgct tcttcactac tttttgattc ttcagtcttc attacttctt cagtagaatt
     4561 tgattcattt atcgtttctg caaatgccgt tacagatcct aacatgggat tgagaaatgt
     4621 aacgaaaatt gccaaccaag ttgccattct tttaaacata tttttttcgt tcctttcatt
     4681 ttatttttca gtcttctaaa gtaatttctt atggacttcg acaccgaaac atttttatcc
     4741 tcctcaaaac aaaaaaagac tagaacttat aattctagtc ttcatcaacc acttactttt
     4801 cttttatttt tagtagaaat ctacagtgtg tttttgtgac ccgtcactgt aaataataac
     4861 atccaccata aaacctttgt tgtaccactg cgaatttgga tcgctaacct ctgcattagc
     4921 ccaatcatag gcttcatcta cagtgttaaa tacctttcca ctattaccaa tctctccaac
     4981 tacaattgtt ggctcttcag gtgtcgttgt gccaccattt ccgccttgat cgcctccacc
     5041 aggattgctt ggttcttcag gcgttgttgt tcctccacct gtgcttgggt tagaaggagt
     5101 tgtttctcct ccagtatttc caccattacc tggttgtgta ggtgtttgag tagtgccacc
     5161 attgttttct ggtgtacttg gcgttgttgt accaccagtt gttcctgttt gatctccagc
     5221 tggtgttgtt ggtgtcgttg tatttggtgt cgtttgtgta tttgaagtcg ttccttgtcc
     5281 tactggttga ttcggatcaa ctttgtcttt ctcttgaatc actgtttgag ccacagtttc
     5341 acctgcgctg ttttgtacac ttgctacatt gccattaaat actaatttat ttcctgcata
     5401 gattaaatcc acatttgtaa tcttattcat gtccgctaat ttttggacag taatatttgt
     5461 tgcttgactg attgcgctta gggtatctcc ccagacaatt gtgtattcat tccctttaat
     5521 ttctgctttg atttgttccg ccgaacgagg tgtccaaact ttgttttctg ctgcttcagc
     5581 attcgttccc acagcaagac taactcctcc gactacgatt cctagtgcca atactttcaa
     5641 cattttcatt gtttttcgcc tccgatttga ataaatttat ttacaaaaac atagtaccaa
     5701 acttatgtct atcagtcgat atattttttg tagatttttt tattttttga tatactaact
     5761 ttggtactaa aataactaaa aggaatgtca cacatgcaac ttaataattt aaatgaaata
     5821 catgattggg ctcaaaataa tttacttaat cgtaaacaag ctgccaaaat cactggacaa
     5881 aactatgatg ctttttctag ctctttacgt agaaataata tcaaaccatt tctagaaata
     5941 ggcgacgaac ctggtccttc catcattcgc ttatatctaa gagaagatat tgaaaaatac
     6001 gcaaaacagc tcgaagaaaa aagacagaaa cgtcaatgag gcgttttctg tcttggattt
     6061 acaagtaaaa caataataaa cacttttaca acggaggttc tatgaataag aaatacattg
     6121 tttcagttgg ttcaatcgta ctgattgtac tcgctattat tgctttttct gtatccaaca
     6181 aaacagaaac acagccacaa aatacggttc aatcgactac tacaagtcaa tctagcgatc
     6241 attccgtcta tccttcacta aatagtttaa aagaaacgca tggacaagat ttaattgatc
     6301 ttggttcttc tcaaattact actgtcttat ttgaaaagaa aaatattgcg tttattactc
     6361 ctaaagaagg aactgacctt gaattttcta tcaaagtaac aaataccaaa gaaaatttat
     6421 tctccattcc tgacattcaa tataccccta acaagttaaa gattggggat acgtttggtt
     6481 tggctaaagt aaatgaacaa tactttgctt actcactgaa ataagtttct aatagcaaga
     6541 ggaaaaaccc tcttgttatt tttatcacat aatcatttta taaaaacgct tcgaaaatat
     6601 caacattttt gaagaaacta tttttctaat ttctcaattt cttccaaggg attttcgcta
     6661 atgacccata caggcacaga ttgctctcga ggctcccctt cttctaacca ccaacctgca
     6721 taatgaactt cttcctcctc agcattacca tgataccaac caacaaagga ttcatctaca
     6781 acgaatgaaa tttcttcttc tggatcaaat aattcatttt tccatgcttc aattgcttgt
     6841 tctttggtgt acttttgttt gttaaataca ccacaaattt cgtcatctac ataaaaaatt
     6901 tcaaaatcaa aattactcgt tccttttact ggcatcttaa cttttcctcc tttgactcac
     6961 ttcaatagat ataccactca tgttcattcc attgtttact agttaattgg agttttatct
     7021 tagggctttg tgcactcttt ccaaaaactt gagcgtcgtt tttttcttct aaccaaaaaa
     7081 ggtaatttct tacattcggt actaaatgtt tttcctgcat ttcttcatga aaaacatata
     7141 attctgcttc atttttcaac caattcatta ttcattccct cattcacttc ttcataccgc
     7201 cattcagata gtgagatttc tttttgtttc tccacttgtc ctataaaaat cgctgtattc
     7261 aacaagctga tcacacttaa taataaagca atgaaactaa tggttacact tactctttca
     7321 cattttgtca ttttgtttcc ttctttactc gtttgttatt tcttgtaata ggtctaataa
     7381 ctccttcacc gaaatttgca aatggttcgc aaaaatttca ataaaagcac tcgcacacat
     7441 taccagacct tcaactgata tatcactgcg acaataaaat tctggagaaa cactgatttc
     7501 tgattctttc ttacaattga acgaaaaaac tatttttgaa tcaaattccg gtaattcttc
     7561 cacaactatg ttttgggatt ctaccaagaa agggagtgcc tgttctaatg cttcttctgg
     7621 tgtttgagca gatagaataa ttgcttcttg ttcttttatt tcattgttct caaatacttt
     7681 ataacttttt ttcattgtta gttcctcttt cattcctctc tcaattagct aagccgttac
     7741 tggtacgcca ttctctaaat ttagtatcgc taatccttaa tgctcgtcga atatcaatca
     7801 ttcgataacc tattgcacga agttctttat atcttttagc agttagcgtc tctaacgtca
     7861 tttcaggcaa tgggttcgaa tcaaaaatag aattcgaacc gattatttgg gccaattgtt
     7921 gtacttgtaa cagctcttct gaacttaatt cagtgattgc tcgattgcct atcattgcat
     7981 acattttctg tcgaatatac ttcttttctt gtttagacaa catttctatc cactcctatt
     8041 ttcattatca tttgttacct ccacttcagt aaatccacaa ataaatgaat tcatattcca
     8101 agtaccattc ccttcaacta caacttttct cctgtcctct ttcggatatt caagaatcag
     8161 accgtttacc aatatgtttt tcactactac taatttgttt gtataccgtg gaattgctgt
     8221 tccgatatac ttcactttat ttcctacgct aattgtcatt tcctcactcc tattcttttt
     8281 tatccaaaac tttttcctgt cctattcctg ccaacgaata tacttcactg catgcttaaa
     8341 caaaacaaaa tgttgccctg ttttatttcc gtcttttact tgttccacat acagttcata
     8401 tttggtcaca ccttttaaaa cgccagatac tttttttcat ccattgtcac aattagtttt
     8461 ttcatcaatc gattgatact gcactgctca tttttcttgt tcatatagta ttcctttctt
     8521 tttcaaaact agcctctttt gtttactagt tttcatcaat catcaacgat ttaacagatt
     8581 ttttctttcg cagggtttct agcgtcagca accccaaact ctaaaaaagt gcctttgctc
     8641 ccttgaaaaa cgagcggacg attgtactcg tcatcatatg tttttcatac tacctgatcc
     8701 acgcaaataa cctttttttg ttctgtgcca cacccatcca tacgtatcgc ttgagattca
     8761 ccatactttt ttaggtggtt tatccttcct aagtccctgt tagacacgct gattaactta
     8821 gaaagtcctg tttgtatcca attttcaaag atctcccaaa agaaataagt aggtttacgt
     8881 gtatcgcttc tgttcaaagt acctgtttgt acttgagtat ttctactccc ctcgttcaag
     8941 ggattccttc caccagctag cttttccttt catcatcaaa tgttcctgaa aagcatttac
     9001 gtttcgtgtc cgtgtgctta cttattcttt tttcgatagc gattatccgc tcgctactcg
     9061 ctattttata ctttgtttgt tagctcaaat aaaaactaac aaacaaaaaa ggctagtttc
     9121 tttccgtttt atttttttaa ctgtcgataa atagttgtta gtttaattcc aagttctaaa
     9181 accaatacgc ccaacaaaac cacaggaaaa acaattgggg caaacaagta aaatcggata
     9241 gttttcatgt cttccctctt tctttttggt gtcgctgtgg tcgttcttgc cgttcaatac
     9301 ttcgatacat tttttattca ttatttgctc gttttgtttt cctctggact gcttttcttg
     9361 ctttttgaat actttatacg cttttcgtgc ctgaattaat aggtcttgtc ggcgtttcat
     9421 tcgtgagaaa acaatatttg aaaccatcaa atcctggttc gaacaaccac gattcgcaat
     9481 aattttccca tcaaaaaaaa tagttttcac ttgagaaaaa aacagttgga aatcttgttt
     9541 tgttgctaat ttttttgtca aaatcactgt gcttttccct gttgaatcta caatgattct
     9601 tcgaacaatc cgattcgata acttccaacg ttgccgatta aacaatgtta cttcacaaca
     9661 aatatgacgt aagtgattag gggtcatttt catgtgtcca attcctccaa ccaattatgc
     9721 atcgaaattt tgggcaattg tgacagccca taaatctgcg tttccaggtc ttcataacta
     9781 caagaacgct tggcttgaag taatgaatat tctgcttgat ctgatagttt tgttgccatt
     9841 ggatctagat tcaattctct tacacaacat tcccaaaaat tcaatagcgc tctaaaataa
     9901 aatccttttc gattctgaat tgcttttcct tcatgacgag caattttttc attttgaata
     9961 caccgttgat acgttttgct taaatcttct gcaaatgttt cccctaaaat tcgatactcg
    10021 cttccatcag ataaaccatg tgtttgatta tactttcgtt gccgcttttc tataattcgt
    10081 ttactgtcgt atatcatgtt tagctgctct ttcgcttcac gccattcagc tgaactgcga
    10141 acaatccatg tcaaaacatc tttcggaaca aacgtacttt cgctgtaatg agcaataaac
    10201 tctttttgcc aatcatcagc catttcaaca ggactagtcg tatcgatttt tgtatctaga
    10261 ttttctgtat ctagattgtt agaagaatta ttaaataatt gtgcagaatt ttcacatgga
    10321 tttttttcgt caaaatcatc gaaaatcgct aaattatcaa cagattcctc ctcttctttt
    10381 ccttgtgcag aatctacact ggttgagtca acaggaccta atgaatacac atcattttct
    10441 gtcgtatcta attctcctag atacaaccga gctttgtttc tttttcctgt ttgttgatca
    10501 aatcccatat cctctatttc tagtagctca tattctttca attctttttt gattgtatta
    10561 acagaacgtc tgcaacaatt caatgcattg gctaattctt gttccgtaaa ttcaaaataa
    10621 acaaaacctt gactatctac ttgatttcgt tgaatggcta aatctaagcg tgaccgaaaa
    10681 accatatacg cgatttttgc tttatcagac aatgagcgat atttttcact tgtaataaat
    10741 acctttggaa attgatagta taattcattg tatttacgag ttgcggtaaa tctttttgtt
    10801 gccatcgtta ttacacctca tcttgtccat atattaagaa agaattttgg tgttggtaga
    10861 cacgaacatc ttccattgta gcaatcaatt cttttagata aattcgaccg aattcaattg
    10921 ttagtaagtc gtaatcttct aattcatttt tgatttcttc aatttcttct tcagacacat
    10981 tgagtaaaat ggctaattct tgattggtat aagaaatata gacatgtttc ttttcatcaa
    11041 tccgtttttc aattaaggat tcttctaagt actcattcag taagctatag gcgtattttg
    11101 cttgtgcagt catcgcataa tattttgacg attttataaa gaatagtttt ggcatatgaa
    11161 aagttgcttg tgtcagcaca tctgttgcgc gaaatttgtt ttttaccata ttttttcctc
    11221 ctagaaaaac acgcaaaaaa agctctgtat aaatcgaatt acacagagtt tcttcttgtt
    11281 gatatttaat ttctaaaata cgtttcgggt aaaaactaaa cacttactaa tatttagatt
    11341 gtagaatctt cgccttcatt tattcatttt aaagagaaaa agatcaagtg aaatgtgccc
    11401 aaaagtttga ttctattaat ttttaaacac cattcataat atatttccta taatgtaaaa
    11461 agcgtcgata cttattaaag catcgacgtt atttttatag taaatttatc aaaatttttg
    11521 ccaacatttt ttccgatttt tttccctatt tctatctttc cttatctttc ttagttaata
    11581 ttatttgatg ttaatatatc actactaata ttaaaaaaca ttgatttatc ataggataaa
    11641 gagtgatagt ttaatactta aagagacata ttaattttat ttaaaaattg acattaatga
    11701 ctcttaatca gcgggtcgcg ggttcgagcc cctcacggcc cattgggtgc caaacccacg
    11761 gaacaggtaa atgttggtgc gaaagcattt cgaaaacatt tacgctgttc tttttttatt
    11821 tttcaaaata actagggctt tttattttta actcaaagtc cactgtagcc ctttggactt
    11881 tgagttaaaa aagaagcata attatgaaca gtaaagtttc taatataaca ataattcgag
    11941 aagctttgca aaatca
//
LOCUS       DAPCMU010000084        11737 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPCMU010000084 DAPCMU010000000
VERSION     DAPCMU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.087"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(799..2430)
                     /locus_tag="RD744_001847"
     CDS             complement(799..2430)
                     /locus_tag="RD744_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDU0557839.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2444..4117)
                     /locus_tag="RD744_001848"
     CDS             complement(2444..4117)
                     /locus_tag="RD744_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDU0557840.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4130..4447)
                     /locus_tag="RD744_001849"
     CDS             complement(4130..4447)
                     /locus_tag="RD744_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0557841.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4472..5872)
                     /gene="lacG"
                     /locus_tag="RD744_001850"
     CDS             complement(4472..5872)
                     /gene="lacG"
                     /locus_tag="RD744_001850"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDU0557842.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6109..6894
                     /locus_tag="RD744_001851"
     CDS             6109..6894
                     /locus_tag="RD744_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0557843.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6897..7589
                     /locus_tag="RD744_001852"
     CDS             6897..7589
                     /locus_tag="RD744_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0557844.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7863..8234)
                     /locus_tag="RD744_001853"
     CDS             complement(7863..8234)
                     /locus_tag="RD744_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDU0557845.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8634..8864)
                     /locus_tag="RD744_001854"
     CDS             complement(8634..8864)
                     /locus_tag="RD744_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557846.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8958..9296)
                     /locus_tag="RD744_001855"
     CDS             complement(8958..9296)
                     /locus_tag="RD744_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557847.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(10224..10340)
                     /locus_tag="RD744_001856"
     CDS             complement(10224..10340)
                     /locus_tag="RD744_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDU0557848.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(10816..11694)
                     /locus_tag="RD744_001857"
     CDS             complement(10816..11694)
                     /locus_tag="RD744_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDU0557849.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3653 a         2440 c         1919 g         3725 t
ORIGIN      
        1 cacaaaatat tttacactct ccattataac aattgggtta aaaagaaaat agatagtatt
       61 ctacctcata tttatttgaa agtaatatat aaaagacgtt caaattcgaa cgcctctcgt
      121 taaccaaata ttggattcag aatctagttt tcatatataa acagagtatg attaggtgca
      181 aaaattgcac ttttattgca cttttatttt tatttatagt gttttactgt tctgtatatt
      241 gttttaactc ccttgatagt atcgcattct gttttataca ctttacgttg aaaacagagt
      301 gggaataaag agccaacata atatggaggg ggaatcccta taaatcaaca ttcttcaata
      361 gctaaaatgc ttgattttca ttaaaaataa gatggttccc taccaaaaga gactataata
      421 taagttaatt tgtttcattc ttatcataaa aagaagttct cgaaaaacga actgttttcc
      481 gagaactttt ttctatccat ctaagtgctc aaatcatatt cacgacggaa ttctaaggtt
      541 accctttctt aaacaaactt tatgttatct gcacaacttt ttgcaatttt ttcattgctt
      601 cttacgaaat cgttgaactc tctttagcat aatacaaagt gtagatgaag tctggtgctt
      661 tactcgtttt ggtaaaaatg actggaaatc gtttctggtt atttttggca aaggcgaggt
      721 gcaggttcga ttcctgcctt ctacgctaac gatgtggctg aagggaacct ccgatcttga
      781 atcgaaacga tccgcagtct attgtttcac caatgaaaca aaatcaacgt tcgtccaagc
      841 atttcctgaa gccttgctat aaaccccaat ttcacaaaca cctgttgtaa ttggaatgtc
      901 tttgattgta atttttgtcc attcacttgt tttaacaacc gcagtattta gttccgctcc
      961 accataattt ttgatatata actgattagt atcaaactct cccccagaca ttacataggc
     1021 actcaatgtg taagttcctt tgcttatgcc actaacattt tgataagctg atgcctcgta
     1081 tgcttgatcc tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg cttccgtttt
     1141 gatcgtaccc ttagcggttc cttgttgaaa ccaggtttgc caattggaag gactggtcgt
     1201 tggtccatcg ctttcgaaac ttgcattttt aactagattt atttgcgccc ctgcttgttt
     1261 aaacgcacgc aagcttggca aggcgtttcc agcaaaatca aacatcgctt ggttgtccca
     1321 ttcgtttccc atagtggatt gaacactgag ataattcata cctgcttgtg ttgcccaact
     1381 cacatttccg ttgtaccaaa gaggttccca atagaagaag cccgccccgc gattattcgg
     1441 tactgcctga atccgatcgg caagatccag caaatactct gcttgtcctg tcggtgttgg
     1501 ttcatatccc cccgcagctg cttcggcttc accaaaagca tttggcatag tatcggcatt
     1561 cgctgttgta tgcccataag ctgtttcaac tacgatcact tctttaccat accgttggct
     1621 gatcgcattc atattggttt gaagttcatt cattgaaccg tgccaatacg gataatacga
     1681 cactccaatt aaatcaaatg acacacctcg actcgtgatt tcatcaaacc accaagtaaa
     1741 tgctcctgta tcgccaccat cagctaagtg gagcattact ggagttgttt taggttgagt
     1801 gtctcgaaca gcttggatac cagacttcaa aaaagcagcc aatcgatcaa attctccccc
     1861 gccttctccc caagacttgc cataaggcca gagcattccg ctattcagtt cattgccgat
     1921 ctgaaccata tcaggataaa cgtccagctg cttcatttcc gacagaacgt tctttgtata
     1981 gtcgtacaat gccgtattca tttcttcaaa ggtcagacct tgccatgctt taggaagatt
     2041 ctgtttccca ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca ccttaagtcc
     2101 cagatttttt gctcgttttg caaggtcaat cgtcgtttcc agatcatttg tccctgctcc
     2161 ataactttct ccattactat tataaggatc ctgccacaaa cgcagtcgaa catagttgac
     2221 accattattt tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat aatattttgc
     2281 accagatttt tccatatctg acaggatcga aatatctgct ccgcgaataa attcgctggc
     2341 agatgcttca acagtttttg aaacagcccc caaagcccct aacacagctg caaataaaac
     2401 agctttacgc aaccaaattc ccattttcat tatggctcct cctttattga aattggttca
     2461 gaataaactg aacagctttt tcaggatctc tcgttaatcc gatatattct gctccttttg
     2521 tcgcaaccag cttgattcct aagcgatcgg tgtcttcttt gatatcatca tagtaagaat
     2581 tgacttgcgg tgctaagacc accatatcaa aatcctgcag catttcgtaa tgagaaccat
     2641 aagcacccgc cattgcactg atcggtgctt tcgattcaga tgcacctttt tttaaagcat
     2701 tagctaacat cgcacttgtc cctgcgccag cacaaagtac caacacattg atttctttat
     2761 ttgcatcgct gataacttga ctcgctgcca caggtacttc ctcacgtaca tcggcagaag
     2821 caccttcctc ggttgcgatc acctctgctt tttgtgcttc tttctctact aagacagcat
     2881 cgtatgcttt gcaaaatggt agatagatca ggaagtcaac gacgattaga accgctgcta
     2941 gaacaaaagc taagataccc atgcccgttc ctagaaccaa accgatcggt gctggagttg
     3001 cccaaggcaa aacgtacata aagctgttca tgcccaatac atcgacaaaa aacttgaaca
     3061 ggcaaacgtt tacaacaggt gttaacaaaa atggaataaa aaaataaggg ttcaaaatca
     3121 tgggtgccgc aaatagcaat ggttcgttga cagcgaaact tactggaata aaggaagctt
     3181 tcccaactgc cttcaactgc ttagacttag caaagagtag gaagataaaa ggaacaacca
     3241 atgttgcccc agttcctccc atagttccta caaaattccc taaacccacc gtcagtactt
     3301 tagaagcttg ttcaccggaa ttgaacaatt gcagattggt ttccacattg gcatagataa
     3361 ttgccgcaat tgccggctcc acgatcgacg gtccatgaac accgacaaac caaaacagcg
     3421 ccatcgctcc ccagataata gcgattccta agtacccgtc agctgctgta aatagcggct
     3481 gcagcaccgt aatgattgct tctgcaaatg agatttggaa gatttgtcga atgcctaaat
     3541 caattaaaac ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg aaggtttgcg
     3601 aaatcgttcc aggaacttct ttaggcattt taatggtaat atctttcagc acacaaaact
     3661 tatataaatt aactgttaaa aatgcagcga caaaggaagc cagtagtccc tttgtcccca
     3721 tatattccgt tgaaaatcca ccttcaatcg gagagacgct taacaacagg aaacttacca
     3781 cagaggccag catcacagag gtggtgttga ttactttgtt tttcggtaag cgacgattca
     3841 tcgaatcagc caagcagcga gcagtggttg ctgaaacgag taaccctaca acacccatcg
     3901 aataattata gactttccat agccatgtgg agacagattc aggccattgg taaccaaaaa
     3961 tctcaggaac cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct ggcatcgctg
     4021 ttaaaaatcc gtcacgaacg gctcctaaat aactattacg tgagattttt tcaaaaaatg
     4081 gtttcccttt ttcgatttgt ttaatgattc catccatagc gttcttctcc tatccttgat
     4141 tctgtctgta cagtacaata aagtcttgaa tcagatctct cagcaataaa gtggtcatta
     4201 aatgatcctg cccatggata aaaatgaatc ccatctccat ctctttccca gatgcttctt
     4261 ccgctaaaat ttttgtttgc gaattatgtg caagggttaa attttcgtcg gcttctttca
     4321 acgccgaatc aacatgttca aagtttcctt gtcttacttc tttcaataat ttcaataatg
     4381 tactgcgagc gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt aattcctctt
     4441 tattcatctt ttcttcctcc attatcactt attaaataat cgttttggtt tcactgacca
     4501 acttgtacca gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa tcaacataaa
     4561 acaagccata ccttttgtta tacccatttg tccatgagaa aagatccata agcgaccaca
     4621 aaaaataccc cttcacattt accccggctg ttatcgcctt gcttaaggat tccaaataga
     4681 ctctcaaata gtcgattctc ggctgatcca taataatgcc atcttcaaac tgatctttat
     4741 accccatccc gttctcagtg atatagattt tgttgtaatg tgggtaatcg cttttaattc
     4801 gcagcaacaa gtcatataga ccttctggat aaatcagcca gtcccaatca gttcgaggaa
     4861 ttccttcttt ataaatacgt tctccgattc ccttcacttt ataaacagag gtcccttttt
     4921 cacccgtacc gttatgatgg attgcatttt ctccatcgta agctttgaca aaatggcatt
     4981 ggtagtgatt gatccctaag tagtcattac gcgtggatgc ttttttcaat tcaacgaaat
     5041 cttcttcagg aaaatgatag ctggcctgat ttgcttcaca tatctcatca agagcagtca
     5101 aggtttcagt tgaataatag cctaaatagg tagcatctaa taagaaacgg atcgacaatg
     5161 catcgtctaa aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca tatttcgtct
     5221 ctaatgagtg aactacacca atttcacccg gaagttcgtt ttctttgaaa tagttcacta
     5281 cacgggcatg agcaaccatc atattatgaa gacaggcgac gatctttgta aaatcatatt
     5341 tgattcctgg tgggaaaaca cctaacaaat attgattggt tgcaactgga tagatttcat
     5401 taaaggtact ccacaccttg acttctttga attcatgaaa acaaaaaatg gcataggaaa
     5461 caaatgcttc tattgtttct cgattcaaaa aatctccatg gtcaaacaaa cgtttagggg
     5521 tatcaaaatg atgcaacgtc acaaacggcg ttacgtttct tttcttacat tcttcgaaca
     5581 cacgatgata aaaagcgatt ccttctggat ttggtttccc agctccatca ggaaaaatgc
     5641 ggctccaagc aatggataat cttagaccat ttacaccaaa acgttcacac aattcaatgt
     5701 cctttgcata ttgatgataa aaatcacttg ccggatcggg actgaatcgc ccttgttcct
     5761 ctaaaaagtc atcccaagca acagcgcctt tgccaccttc cttagtggct ccttcaacct
     5821 gataggctgc tgtggctccg ccaaaaataa aatcttctgg taatttaagc atgtacatct
     5881 ctcctcaaat ctataatcag ttttcgctct ttctaactga ttgcattgta aacgcatcca
     5941 aaacatttgt caacaaaaac aaacacaaat aatcataaaa accagaaaac agcaataaaa
     6001 tctattgtaa caaacataaa aaccacccta aatgtttact tttgttcgaa ttagattgac
     6061 tgttgtccgt ttatatgata gattatcctt aaattagagg tgaagacaat gttgaaaaaa
     6121 gaaagattgc ttactatcgt agaaatggtc aataaaaaag gaatccttac cgttaatgaa
     6181 atcatcaatc aattagatgt ttctgatatg accgttcgca gagatttaga tgaattagaa
     6241 aaatcgggca agctgctgcg tgtccatgga ggggctcaaa gtctttccta cactttagat
     6301 caagaacttt ctcacactga aaaagccact ctgcagattc aagaaaaaaa agaaattgct
     6361 gccacagcag cctccttgat tcacgaggga gaaacgattt tcttaggacc aggtacaaca
     6421 atcgaactat tggcgacgca tttactgaac aagaaaattc ggatcattac aaataattat
     6481 ccagtttttg atattcttgc tcaagcagat ggaccagaaa ttctactgat tggcggtgat
     6541 ttccggaaaa ataccggcgc atttgtcgga ccgatcacca acgacaattt aagaagacta
     6601 aagtttacaa aagcatttat cagttcaaat ggcgttcaca acgaagcgat ctctacttac
     6661 agtaccgaag aaggagaagc acagcagatc gctctaaata attcccgtaa gaaatatcta
     6721 ctcgttgata ataaaaaatt caatcgagat gatttttatg tgttttataa tttgcatgat
     6781 ttcgatctgc tcattacgga tcgccagatc tcgcgggagg ttcaagagca ctatgcacaa
     6841 tatgtcgatc tgcaaattgc tgattcaacc gaaaatacca ctataggagc gtaactatga
     6901 aagggattat atttgatttc aacggaacaa tgtttttgga ctctcatctg catgaagctg
     6961 cttggctgca tatgatacat gatcatacaa aagatggttt atcagacgag gatattttaa
     7021 aaaatattca tggtcggacg aatacggaga ttttgaaaca tttcatttca gagcatttga
     7081 ccgcctctga gatcgccgta ttgtcagaag aaaaagaaag ctattatcgt atgctttgct
     7141 tgcaaaatga aaacgagctt cagttgacaa aagggcttga agcgactctt gaccagctga
     7201 ctgaccaaca gattccatta acgattgcca cggctactgt caaagagaat gttgcttttt
     7261 attttgatat tttcgattta gcccgctggt ttgactggga caagattgtc tatgacgacg
     7321 gctctttccc tgggaaaccg caaccggata tttttcttaa agcggcagac aaactgagct
     7381 tagctccggc tgattgtctt gttatcgaag atgcttactc aggattactt gctgccaaac
     7441 gtgcggggat cggaacaatt attgcaattg atccattcgg taaaaacaaa acagtatttg
     7501 aacaggcaca gctaaataaa gatggaacca tcaaagattt tactggtttt tggacagagc
     7561 acctgtctgc cacatccgtt aattcttaaa cgattcaact aactaccaaa gaaaagcaac
     7621 tgaaatgcac acctcctttt ataaaaaact gccagtgaaa cgacatgaaa gatacactgc
     7681 tatatcctta gtgaaatata gcagtgtatc tttgactttt tcattctttt aaccatatga
     7741 cgcgttcatc tctatctata attaacatta aaaatagtgt tttcacatta tttctcaaac
     7801 caatcttaaa aatcatcagg agtacttttt tgatatactt ttattaatgc tgggcgtaat
     7861 ggttaaaaga tttccactag ttcctctcca ccaagctgag ttgaaaaatg cttatctgta
     7921 aacaacatag acaaagagga aataaacgtt tccgtatctt tctctgtccc aaggatatta
     7981 attaattttc ctactaggta agtatcatta aacttaagat ctgccttcac atcaaaagtt
     8041 ttgataaaag cttttatcat gcgtacaatt ttgtctttat cgccataatt gatttcacca
     8101 ctcatatcaa tatagtcact aatccctgaa gaaataatgg caactgcttc ttctttcgcc
     8161 ttatcagata attcatttaa tgactgggat tgattgatga cttcttgagt tttatctgtt
     8221 acgtaatctc tcattatcct atcggcatac tccgcaaagt aatcttgaaa aaattcttct
     8281 acagggtaac caacttcgtt ttcccaacca ggagaaattt cattatcagt acttattaaa
     8341 tctattaaaa accaacccgt tctctgagtg ttgattctat aatattttta atttcctata
     8401 taaatcaaaa atattataga atcaataagc taattaagaa acaaacttta gaaactctag
     8461 aagaacttct gaatttatat agaagtgtgt agctacttac taaatataca aaagttaaaa
     8521 taaaatactc tggatactct ttatactgca ttaaagttag taatattaac agcaaagata
     8581 tcgaaaaaac aataacataa tatgtgtcaa aatttttcat aataagaact cccttactgt
     8641 tgcatactaa cataatccaa aaactcttct acttcagcta aagacattcc actttcttta
     8701 gtccaaaaaa cagttttttc tatcgatcct tctctgtaaa ctgtaaatgt cggagtcgag
     8761 ttaattgagt agcgactctt gaattctttc cactcatttt catcttctct aacttttgct
     8821 acattcacgt aataaatatt ttcttttccc ttttctacca gcatatcgaa tagcttcctg
     8881 cttttgatga tcgatttctt gtgctacatc tagtaaattt tcatcaattt ctatatcata
     8941 aagtatttca ctagtgtcta catccacaat atatactttt tatcagtagc aatttttgag
     9001 taatccgtta aaatgatctc agtctcgtca ctttcatcag tataaatata cgttaacaat
     9061 gaactcacct tttcaatcgg tgttagctct aagcctactg tattttttac tattgagtca
     9121 ttatttacca ccataacttc gtactgtata taccatccta tttggtttcc atcaatatca
     9181 gtaattggct cactagctaa tggagtctcc ggaacaatat tttcctgata tttcttaaac
     9241 tcttctgtag cacgaaaaag gttatcatgt tcctcacttg aaactggttg agtcatagaa
     9301 aatgttactt ggctaaacag agggaaaata aacactacaa tcaacaaagt ataaaaaaaa
     9361 gcccttcttt ttcatttttt atcttccaat cacaaattga aaaacttatt cctgcaactt
     9421 tgagtctagt atcacaatcg actgactaca actattcatt tacattctac tttaagttcc
     9481 tccttaacca ttaattttat taaagatact tgtaatagtg attctcacaa aatttcatga
     9541 aaaatacttt attactgtat ctaaaattcc aatacacaac ttatcgacta ttatctgaaa
     9601 accaaaactt attattcctt gatgttattc tagcaatagt aactgctttt ttattttttt
     9661 gcatattttt tcggcgttac tttttaaagt taactttttc gacaaataga aagagatacg
     9721 aatcactgat ttttttgaag aaaagaaatg gagtgagctg aacgttagag atattgggag
     9781 ctttcgtaac tgcttattaa acgataatgg ttatttgcaa ccgtattgct cgcttctaca
     9841 tgaaatattt catgaaatat ttcgcaaaac ctttgattat gtagttacgt ttccattctt
     9901 gactaaaaat gtttcaaaaa cgaacaaagg tgttaaaagg taaagtgatc aatactactg
     9961 gtcaaaataa gaggttgaaa gggaattgaa aaataactac agataatact tttataaacg
    10021 tacgtgcttt gtggttctgt agttaactat ataactggaa ttagggaatg gtatcaattt
    10081 gaaaagaaag attctaccaa tttatcatat gttaaaaatg gaaaatcaac agcagttctt
    10141 tcggaaacct tacacaaaaa ccgattattg aaaatagccg aaaaacgttt ttcggctatt
    10201 tttttaatta agtttccttc ttcttacctt ttcccactat tccgtttcac atctttgatg
    10261 atcttcagcg cacgttttcg tgttttgata tttgggcttt tcaagcggcg gtatgcactt
    10321 gagatatctg ttttttccat atggacgacc cactttcttc tttgattgta gtcatgctaa
    10381 aaaacacaaa tcgaaacaat tacgatttgt gttttaaatg gtatcaaaag ttaaaatgaa
    10441 agtcaacttg ttaaaacagt cgatcagact agctgattga ctcctatctc tggaaataaa
    10501 ttttcattat tacgttttag ctacctaaaa aaggccctct tgtccgttaa ctttaatatc
    10561 tattgttata gctagtttac ctattgaacg tactgtgtgt tcgatagaac actaaaaaag
    10621 aggctgggac agaagcgttt aactccaaga aataagaaga aattcacgaa aattgctttt
    10681 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    10741 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt ctcacgctct tttaaactat
    10801 ttcgaagtac taaacttatg aagtaagaat acctaagcca gagccaatgt gctcgcctgc
    10861 aagcaattgc tcttttgctt gtcgctgtaa actcgtactt tctggataat catgtatttg
    10921 ggcatctact cgtccaaaga tccaagggac tccactatcg tcctgttcat caactgataa
    10981 taatcgataa tcggctgcaa aaagacagat ttttgactct tttttcaacc atttagtaag
    11041 attgggtgat agtaaccaag tatctgtgta catcacttgg taatggtaat caggaaaata
    11101 aagttcaaaa aaacgaatgg cactttcgta actatctctt atcttattct gagataaatc
    11161 tgcatcggaa ggaatatgaa gagaaaggca ctgctttcct tctaatctta gatccgtgta
    11221 ccctttcgga aaatatgtca tttcgtactc caattcacca atccgaaaga tcaagccact
    11281 agtgtaatgc caaatccaaa aaccacggtc aaagcgatag cttcctgtta cttggaaatg
    11341 ttcatgaata aatctagtaa aagcattcat tgttgccaaa tagatagttc ttggaatctt
    11401 caatggttga taaatttttt tccaactttg agaagctgca tatagataaa tagctaatgc
    11461 tgcaaaaggt tcctcttcat agactttgtc tattttcttt gcagtcgcct ttgctttatt
    11521 aggatccatt agttcatgac tatattgctc aaacacctca tcaaggatgt tttttgcttg
    11581 tacaatcatt tgctctataa cgactttggg aagagttaaa gtttctgcca gaatttggat
    11641 ttcattgctt attttcttca tcaaatttcc ccttttcata tctttgaacc tcattgttta
    11701 aatgactgat tatccggaca ccatatggta taaacat
//
LOCUS       DAPCMU010000085        11457 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPCMU010000085 DAPCMU010000000
VERSION     DAPCMU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.088"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(106..768)
                     /gene="trmB"
                     /locus_tag="RD744_001858"
     CDS             complement(106..768)
                     /gene="trmB"
                     /locus_tag="RD744_001858"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDU0557850.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(838..1620)
                     /locus_tag="RD744_001859"
     CDS             complement(838..1620)
                     /locus_tag="RD744_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDU0557851.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1824..3035)
                     /locus_tag="RD744_001860"
     CDS             complement(1824..3035)
                     /locus_tag="RD744_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557852.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(3028..3765)
                     /locus_tag="RD744_001861"
     CDS             complement(3028..3765)
                     /locus_tag="RD744_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557853.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            3903..4337
                     /locus_tag="RD744_001862"
     CDS             3903..4337
                     /locus_tag="RD744_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDU0557854.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            4330..4674
                     /locus_tag="RD744_001863"
     CDS             4330..4674
                     /locus_tag="RD744_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDU0557855.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            4854..5864
                     /locus_tag="RD744_001864"
     CDS             4854..5864
                     /locus_tag="RD744_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDU0557856.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(5933..6877)
                     /locus_tag="RD744_001865"
     CDS             complement(5933..6877)
                     /locus_tag="RD744_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0557857.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6861..9581)
                     /locus_tag="RD744_001866"
     CDS             complement(6861..9581)
                     /locus_tag="RD744_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0557858.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(9578..10798)
                     /locus_tag="RD744_001867"
     CDS             complement(9578..10798)
                     /locus_tag="RD744_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDU0557859.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(11079..>11457)
                     /locus_tag="RD744_001868"
     CDS             complement(11079..>11457)
                     /locus_tag="RD744_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDU0557860.1"
                     /translation="EKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIE
                     IVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKK
                     ADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         3478 a         2273 c         1870 g         3836 t
ORIGIN      
        1 cattttaaat caaataaatc ccagaaaata cattacatct gtaatgttca ttttctgaga
       61 tttatttatg gatattaatg tttttatgaa agcagaaagt tagtttcaca gacttttctt
      121 tttggtctta tcttgaaatt gagcttctac tcgataaata cgttgacctt ttgaagaaaa
      181 tttttcttca tactccgtca taatattgcc ttcaaattga ctttgatgta aatctaacca
      241 tacctgtttg aggatcatgc catattgaga aaaacttgcc agagaatact caaacaatcc
      301 ttgattatct gttttgaaat ggatttctcc atttggacga agaatctgtt catctacagc
      361 aagaaaagtc ttatatgtca atcgccgttt ttcatgacgt ttttttggcc atggatcgga
      421 aaaattcaga tagatttgat ctacttcgct gtcagcgaaa tactctgtca acgctgatcc
      481 atccacatgt agaagtttca ggttaggaag cggttgttcg atcaacttgt ctagtgcaat
      541 cgaaacaaca ctgacctgca tttctactcc aatataattg atttcaggat gggcttttgc
      601 cattccagtg atgaactggc ctttccccat accaatctca atgtggattg gatgatcgtt
      661 ttcaaacaat tcattccatt ttcccttcca caatgttgga tcactaatta caaaattcgg
      721 gtgagccgct aacatttccg ctgctcccgg acgatttcgg acacgcatta tttatctcca
      781 ttcctttcct ctaattaaaa aggtctggcc aaatctccgc agagtttggg ccaaacctca
      841 gccactgaaa attcgtttta attgtaaaat ttcttcgttc atccgtcgat gatcttcacg
      901 tttgcaatat ctggtgattt cttggagcaa attcatggcc gcataccaat gaaatttttc
      961 aagcatttcg tcagtcgggc gtatgccata actgatcagc cactgattcc agctagacag
     1021 tggcacataa tttcctaaaa tcgttgcaat atccaccgca gggtctgcaa acatgattga
     1081 atcccaatcg actagatata aataattttt acatactaac caattacgat ggtttacatc
     1141 tccgtgtact actgataagt tttccttatc tattgacggg atctgctcac gaagaaaacg
     1201 atggacaatt tgcagaaatt gatttttctg gagttcttcc ggtaattctt tttcatattt
     1261 ttgcagcatc ttctccggcg tcatgacacg accaccgata cgggtcagca tggttttaag
     1321 agaatttgaa tgatgtaaat ggtacaaaac atcaatcacg tcgtttcttt gaccaatctc
     1381 attaggagtc agcaagcgac cttccagcca ctcttgtgct gtcaaagtat cgccattccc
     1441 cgttcgtttt gtccaaacaa gttttggtgt aattccttcc cttgaaagag cagccaacat
     1501 cggagtcgtg tttcgtttaa taaagacttt gtcctcatca cgcatgccta tgtatgtttt
     1561 tcctgtgtcg cctttgatgg gacgtaaccg ccactcttgg tctaactgaa actccatcat
     1621 gcattctccc gtcctcttac tctttgtttt gcagtactta ttattctagt cgcctgaaca
     1681 ctacacgtca agcacatata cgttcttttt catttaattg ccattcaaaa aaccagcttt
     1741 tctcaaaaaa aatagaacga acagcattct tactgtcaac gctctatttt tcattctcat
     1801 ttttacccta cacagctttg ttttcagtct gacatcttct tcaaacgagt tggtacatat
     1861 agtttaacaa agagcatagt aacaatcaaa taagccgcta gcggaaccca tcgctgctgc
     1921 ccatctaata cgactgcagc aatcaaacca aaaataaccg acgcgataat taatacccat
     1981 gtgagtagat actgaatcgc ctgcttcttc tgattcttat ggattggata aagctgaacg
     2041 agaaccatat agcggaactg gttcgctaaa ggtaacagtt ggaaaccgat caaatataaa
     2101 aataagaaac cgataatcag actaaaataa aagtcgacta ctccggcaat caataaccca
     2161 cctataatta ctaatcggaa ataaagacca ctaaaatcag aacctcttgc caatcttctt
     2221 gcaaacaagt aaagataagt gttttttgat tctcttttaa tatgagtcaa tataggatcc
     2281 aaatagcgtc ttcttttgat ctttgccgta atttcaggaa catctgtgaa taaattgata
     2341 aattgataga tctgatgcaa cctatgttct tctacatgca ccattttctc ccaatccaat
     2401 cctttttctt gttgcttcca caaaaaccag taatatagac ttgcctgtac taatgcacct
     2461 gctattcctg cccatattag ccaataaaat gatagaaaca aaaagatcag agaagaaaaa
     2521 aaccagccta atttccaata acgaataact tgttgatcta gtcgaaacaa ttctgctctc
     2581 ttgatttgca gatgactgaa tttcaaactc cataatatga tcagatagac gaaaaacatc
     2641 gaaaaggatt gctccgtact aaccaccact aatggcatgg ccaatccgca tgccaatatc
     2701 aaaaagatca acggaaaaat gcaagaatag cgaaaagctc gttccaaata ggtttgcatt
     2761 tctttttctt taggaagtaa aaaaacaata tctgctgcct ctgcaaaagt agccatctta
     2821 cccacgaata aacagagcat ccaaaataca gagattatga gccctccaat tgcaaacggt
     2881 ggctgcaggg ttttcaacca attagaataa tataatccca caccgccaat caagaaaaga
     2941 caaactaaaa caaaatgatc gttcaataca taacgcatat atttcatcat tttcttttga
     3001 tgtcttgcta aacgtttttg aaaaaaacta ctcatgtgcc tgctcctttg ttaaagcaat
     3061 atagatttcg tccaaagaag aatccggcag ctgaaattct tttctcaaca tctcaagaga
     3121 accgtctgct cgtatctctc cgttatgtaa gaccacaaaa cgatcacaat atttttctgc
     3181 agtagctaaa atatgcgtag acatcaaaat accggctcct tcttttttca tttctaccat
     3241 caattcaagc aatgcattga tagctagtgg atccaagcct aaaaatggtt cgtcgatgat
     3301 atataaagaa ggctcgatca aaaaggcgca taagatcatc actttctgtt tcattccctt
     3361 tgaaaaatta gctgggaacc agtcaagacg attagataac cgaaaagtct ccaacaactt
     3421 ttctgctctt ttcattgcta tatccatagg tatatcgtag gccatcgcag tgacttcgat
     3481 atgttctctc aatgtcaatt cttcatataa agaaggagtc tctggaatat acccaatttg
     3541 tttgcgatat tcttccgtat ttttctttaa ggatagatga ttgatttcta tcgttccttt
     3601 tgaagcttcc agcagaccga taatgtgctt gatggtcgta cttttccctg ctccgttcag
     3661 tccaatcaac cctactatct ctccaggctg tacatcgaaa ctgatatctt ttaacacagg
     3721 gagatgtccg tacccccctg tgacatgatc gataattaaa ctcattcttt ttcctccgcc
     3781 atttattttt atttattata ccacgaagaa aatgacggta ttttgttttt ttccttacaa
     3841 tagctttaac ttatggtaaa gttaagctga agtatttatt ctttatagaa aggatgtttt
     3901 atatggaaaa ctgtattttt tgcaaaatca tcaatcaaga aatcccaagc tataaaatat
     3961 atgaagacga caaggtctat gcttttttag acatctctca agcaacgaaa ggacacacat
     4021 tagttgtgcc taaacgtcat gtagcagata tttttgaata tgatcccgaa cttgcaggag
     4081 aggttttttc gcgtgtgcct aagattgctc aagcattgga aaaagctttt ccagaaatga
     4141 aaggtcttaa tatcttgaat aacaaccgtg aactagccta tcaatctgtt tttcattcac
     4201 atatccattt agtccctcgc tacagcaaag aggatgattt ttctattcac tttgtcaatc
     4261 atcaagatag ttatggatcg gaagaactaa aagctattca agaaacaatc gtaaagcagg
     4321 tgtcctgcga tgattaaaaa ctttttcaaa ggactgttat ttggagctgc tgctggcact
     4381 gtcggaggtc tgttattggc gcccaggagc gggaatgaaa caagaaagaa actgatcgaa
     4441 gaattggaag aagcaacaac tctgacaaat gatttaaata acagcctgaa tcattttaaa
     4501 agcgcattga tcgaaacaaa agaaacagct gaatctgtga ttcctgcttt ccaagaagca
     4561 atcaaaaaag atatcgaaga ttttaaattc caagccgaac cacgtatcac acaaatcaat
     4621 gaacaagtag aaaaactaaa agctgatctg ccagagtatc ctgaacaaat ataataatga
     4681 ttattttctc aacagattta actatataag tgagaaaagt ttgcgtattt cctaggaatg
     4741 tgatgaaatt ttgaattttg tttgaaacta ccacgacaac gtttttaaag gaaatgggat
     4801 atggtatagt taatatataa aaataataac taattacagg agtgcaaatt ttaatgaaaa
     4861 aaaaatcaat tattttggca gctactagtg cgttggctgt tttaactcta gcagcttgtt
     4921 caggcgatac taataaagat atcgcaacga tgaaaggcgg aacgatcact gtatctgatt
     4981 tctatgatga agcaaaactt gaatcatcga accaatcctt agtacaacgg atgatcattt
     5041 ataaagtatt caataacaaa tatggcgata aagtaaccga taagcaagtg gatgcagaat
     5101 acgacaaaca agcgaaatca ttaggtgata cttttgaatc acaattagaa gctgctggat
     5161 atacgaaaga tacttataaa gaatatattc gtaacaattt agcttttgaa gctggcttga
     5221 aagctcacgt agatattaca gatgacgatt tgaaaactgc ttggaaatct ttccatccag
     5281 aagtagaagc acaaatcatc aaattatcaa gtgaagatga agcaaaagat gtgaaaaaat
     5341 cagcggatga tggagatgat ttctctaaat tagccaaaga caaatcaact gatacagaga
     5401 caaaagaaga tggcggtaaa gttaaattcg actcaacgac aacaactatt cctgcagaag
     5461 taaaagaagc agctttcaaa ttgaaagacg gtgagatctc cgatgttatt actacaacaa
     5521 atcctacttc ttatgcgaca gaatactacg ttgtcaaaat ggtgaaaaac caaaataaag
     5581 gcaatgacat ggataaatac aaagatcaac taaaagatat cgctactgaa actaaactaa
     5641 gtgacaatgc attcacaact aaagtaatcg gtgaagaatt aaaagatgca aatgtaaaaa
     5701 tcaaagatga tgcctttgaa aatgtgttga gtgcattcac aacaacttca tcaagtacga
     5761 aagactcaag tgaaactaca gcttcaacaa agagttctga cacaaaatca actgattcaa
     5821 caaaagaatc ttcaactgaa gaaacaactg attcatcaaa ataaaagaaa acaaaaaaag
     5881 cgaagactta tgtctcgctt tttttgtttt gaatgaaaaa aataaatttt tgctataaat
     5941 cttttggtac atagaaactt cggttgtcca taccgaaaat ccgatcggtg tattttcctg
     6001 gttctgtttg acgaatcgaa gtcaacatca tctgcatcga agcatcgata ttgtcgattt
     6061 gatgtaatat ttctgcttcc attattcttg gacgtacagg tgagccatat tctaataagc
     6121 catggtgtgc taaaaccata tgacgtaata caagaacatc ttcgtcagct tcgctgattt
     6181 tcaacgcaag acaggcttta gttatttctt cgtcaatcaa taccagatga ccgatcaaat
     6241 ttcctgcaag cgtatattct gtcgacatag gaccggaaag ttcaatcacc tttcctaaat
     6301 catgcaaaat gataccagca tacaacaatg ctcgattcag ctcaggatat tcatcggcga
     6361 ttgatttccc taagtgaagc attgtcaaag tatgataagc taatccactg gcaaatgcat
     6421 gatggtttct ttttgctgcc ggaaactcaa aaaattgttt ttgatattta ttaagtagat
     6481 agcgaacaat acgattccaa tgcgcattgg tgatctcaaa caacaattga ttgatttctt
     6541 cttccatttc ttctttcttc aaaggggctc tttccatgta taaactaggc tctcctggct
     6601 catctaccct tgttaagcgc atatgaagaa tctttacttg agggtttcct tgataaacct
     6661 ctcttttacc gtttagaaaa acgactgtgc cggctgtaaa tttattgatt tcctcctccg
     6721 aagcatccca gtatttacca tcgatagtac ctgaagtatc ttggaaagta aaagcaataa
     6781 atttttttcc gtttttagcg attcttacat ctgcactttt gatcaggaca aattgttcaa
     6841 actgctcatc gattactaaa tcacgaattt ttttcattat ttgctccttc taattgtttt
     6901 acgatttggt tcatctcatg gtaatagctt accatttctt tatcagaaga aaaacaaatt
     6961 atttgatact tttctccaaa tttagctaaa agttcagcta attgatactt tcgctgatga
     7021 tcataatgaa gccagccatc atcaatgatg atcgggcata tcgaacggtt agcttctaat
     7081 gagaggtaag caaatctcat cgccataatc agctgatctt tcgtacctgt agataactca
     7141 tagatgggaa attctgtata tccgtctgtt aaagtcagtg tatcattttt cagtaatatc
     7201 ttctgataat ttccttttgt taaaatacct aaataatcac ctgctgctct taacaactgg
     7261 ggtaattgtc tttcactcat ctctgttgaa agatcaccta agaatgtccc ggcgatttca
     7321 agtgctcccc attcctccag taattcctgt aattttgctt tttgccggct agcttgttga
     7381 taaagttcgt ccaatgtgcc gtctctttgc aactcttcta cttgaagttg gagtcgttgc
     7441 cgattttcac ttgcttcttt ttcttcttgc tggagttggg attgcttttc tttcagttgg
     7501 cgcagtctta cagataattt ctcttttgtc agctcttcag gaaatagtgg agtaagcatt
     7561 tccgataatt cattgtatct tttcatctgc tgttcggctt gtttggcttt ttgtaaaaag
     7621 acaggtactt ccgaaggata agataagcct gcttgagtca attgttcgct gtattcttgt
     7681 atccatactt tttgtttctt tttcaattga ttgatttctt gcttcaataa agtattttgc
     7741 tgatagcttc tggcaaattt catctgttcc atctcttgta taaaacggga aagcaatacc
     7801 attttttctg aaattgtctt atcttgtatc ggtaaccatt ctttaacaaa ctcaaatttt
     7861 tggtcgattt gtcgtagttt ttcttgtatt tcctgttctc ttttatgttt ccgctcaaat
     7921 acgccatata atttctgata atctatgact tcctgtccat attctttcat gagtttgagc
     7981 gtatccatct ttcctaagtg ccgatgatcc gcaaaacgat atacttcttg ctgaagaatc
     8041 tgtttttctt ctccagttct cttttgtttt tcttctgctt cagctaattg agctaaggct
     8101 agatcaagtt gtcctagttt ttcttgccac attcctttta tctcagagaa atcattttta
     8161 gttttaattt ttggttgcct tttgtagaag aaatagccaa tcatgcctgc accaattagt
     8221 aaaaggatca ctttcaaggg cattggtaca aatatacctg caatgattat tgctccgcca
     8281 gccatcagca tatacggttt agatgaagag actggtgcta tctctgactg attttgtaat
     8341 aatgctggat aggtctcttc caagtcattc acttctgctt caatcatctc ttgctgttct
     8401 ttagctattt ggagacgcac agaaatctgg ctttctagat cagcaatcct tctttcttca
     8461 gccagaagat gcttccattc ttcttcatat aaaaattctt ccggtaacgg atttttctgc
     8521 caatgccacc tttgttccaa aatcttcaat tctgaatttg tttctctagt ctcaatttgt
     8581 aatctttgcc attcttcgtt taattgttcc gctgtaaatt gaagttgctg gagatctttt
     8641 agttcctgct cctgctctaa ataaaaataa tatctggctg actgctgatc catcccgcta
     8701 tgcttttcca gtgttgcttc taattcttcc aaacgctcat ttgtttgctg ataccgtttg
     8761 gagaaagact ccagatctaa aagaagttgc tcatcaggaa gacctgtagt gctgtttttt
     8821 ttgagagatt gccattcttc atataaagaa aaattaagtt cttgctgtct ggctaatgaa
     8881 aattgtttgt tcgcctcttt taattgtgcc aataactgct gctgttgcga ctcgtattcc
     8941 tgttcttgtt ggatcaactt gccaaaataa gcttcctgtt cttgtttttg atgaattttt
     9001 tcttgcagct ctttccattc atttaatttt tgattgatta gtaatttttg gccttttttc
     9061 ttgaataatt gttgtgcttt ctgataatat tcttgccgct tttggaatac ttgagaactg
     9121 cccgtgatgc ctaaagaaat cagcgcatca tgtagctctt tttctctcaa atgatctagt
     9181 tccatcaact gctcttgctg gaaagtaaat acctgctgga ataactcttt cgtcaaaggt
     9241 gcgatcagtt tttctagtaa ctcatcactt cccacttgat cccccagcca caccttcgat
     9301 tttccgcgat tttgctgctt gtagcgctcg acagcaaatt caccatatac aggatgttta
     9361 agccatattt tgccgccata ggctgagccg tcttttggtg tataatcctt ttttttacgg
     9421 ccttttgaag ggaaaccaaa taatattgct tgaataaatt gataaatcgt tgattttcct
     9481 gcttcgtttc ccccatagat cagctgattg cctgaataaa agtcaaaact tttttgacgc
     9541 cattttccaa aagcagcaat ctcgatcctt aaaatcttca ttcctgatcc tcctcaaata
     9601 aaaaatcttg attaagcagc tgcttggctt tttcaagtgt ttcttcttga tattccggca
     9661 gttcgctcaa tattctagcc gcttcttgat gactatatac ttcatttaaa atttgttgga
     9721 aatcttgagg tgtctgatag tattgaaata actgttccat caatttagca gaagcagcaa
     9781 gtggggtctt cgtataaaaa gtattctcta tgagagaaat acgccaaata aagaaatcat
     9841 ttatcctatc tgagaattcc tctaacaaat agtctaatag ctctcctgat tgtatccgtt
     9901 ccaaaacatc tgtattgaga tggtcataat cttttaattt tatctcaatt aaactaaatc
     9961 catcctctac ttttgaaagc tgctgacgaa tgtgaacaat gacctctttt gtcgttcttg
    10021 ctttcctcaa tgatatttcc tttgtcttcc aatagacctc tgctacttta atcggatgta
    10081 tttggcaact tcctttactt aattcaacta gtagaataga tgtagaagct tgttcctttt
    10141 tagtatgacc ttgaggcgca cctggataaa caattgtttg cttttcattt aagaccgttg
    10201 gcacatggat atgccctaaa gcccaataat cgtatccctt ttcttgcatc tttgagggtt
    10261 ggaaaggagc atagtttcct ttttgagaga cgccaggttc cccgtggtac aacccaatat
    10321 ggtaatctgt gagttctcta gaaggaaatt ccatcacttt gtctttttga atccacggtt
    10381 gacgataact aaatccactg agagagacag tttctccact ttttatcgtt attttttttg
    10441 tctctaccgt ttcggatgta aataaatgga catttttagg aaaggagaac caataccgct
    10501 ctttttgata gaaatcatga tttccgaata ttataaatac aggtatatgg ttcttttcta
    10561 accgtttcat ctgttctgaa aaatgtttct gtatttttaa tgaaggacga ttttgatgaa
    10621 aattatctcc tgcaagtaaa acaaaatcaa cgttatttat tattgcctgt tcaatgatat
    10681 ttgataaaac ggtcagattc gtcttcaata gtttttcctg tacagtttta tcaagagttg
    10741 tcagtccttc aaatgaccga tccatatgta aatctgccgc atggataaat ttcaccatta
    10801 aaaatttcct cctttttttc ttttatcata ccattttcaa tgtttttttt catcaataac
    10861 cgaaaatttg ttcgtattat gcaaagaaat tacgataaat ggccgacttc aaaaaataag
    10921 tttattcgaa aaaaagattg tgacatgatt tttgtcacaa tcctaaatat ctatgttata
    10981 ttcgcttcgt ttgacactga agaagtacta tttgtactgg aatttatgct ccatttttcc
    11041 gtatttagaa ttctatgttc tatactacct taattagttt atatggtaag aattctttca
    11101 aatgcttgag atactccgtc tttatcattt gaaacagtga cataatcagc ttttttcatg
    11161 atttcttgat gactatttcc cattgcaaaa ctaatatctg cactttcaaa catagataag
    11221 tcatttaaac tatctccgat cgcgattctc aaaggtttct ttaaattgta gattgattcc
    11281 aaacgatcaa acaccacacc tttagataca ttttgaggta cgatttcaat atttacaggt
    11341 gccgattcag taacagacac atttagcatc tttttcaaat ggttaaatat attagctttc
    11401 aattgacgat taggaataaa aataaaaaat ttgtaaatgc tgatagcatc cttttct
//
LOCUS       DAPCMU010000086        11429 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPCMU010000086 DAPCMU010000000
VERSION     DAPCMU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.089"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(31..576)
                     /locus_tag="RD744_001869"
     CDS             complement(31..576)
                     /locus_tag="RD744_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0557861.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(588..1280)
                     /locus_tag="RD744_001870"
     CDS             complement(588..1280)
                     /locus_tag="RD744_001870"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDU0557862.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1299..1583)
                     /locus_tag="RD744_001871"
     CDS             complement(1299..1583)
                     /locus_tag="RD744_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557863.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1596..2159)
                     /locus_tag="RD744_001872"
     CDS             complement(1596..2159)
                     /locus_tag="RD744_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDU0557864.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2387..3049
                     /locus_tag="RD744_001873"
     CDS             2387..3049
                     /locus_tag="RD744_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDU0557865.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3173..4369
                     /locus_tag="RD744_001874"
     CDS             3173..4369
                     /locus_tag="RD744_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDU0557866.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4470..5846)
                     /locus_tag="RD744_001875"
     CDS             complement(4470..5846)
                     /locus_tag="RD744_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0557867.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5883..6749)
                     /locus_tag="RD744_001876"
     CDS             complement(5883..6749)
                     /locus_tag="RD744_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDU0557868.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6838..7863)
                     /locus_tag="RD744_001877"
     CDS             complement(6838..7863)
                     /locus_tag="RD744_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0557869.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7881..8555)
                     /locus_tag="RD744_001878"
     CDS             complement(7881..8555)
                     /locus_tag="RD744_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0557870.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8739..9632)
                     /locus_tag="RD744_001879"
     CDS             complement(8739..9632)
                     /locus_tag="RD744_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0557871.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9706..11403)
                     /locus_tag="RD744_001880"
     CDS             complement(9706..11403)
                     /locus_tag="RD744_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0557872.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3386 a         2515 c         1962 g         3566 t
ORIGIN      
        1 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
       61 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
      121 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
      181 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
      241 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
      301 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
      361 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
      421 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
      481 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
      541 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
      601 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
      661 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
      721 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
      781 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
      841 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
      901 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctggtttg
      961 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
     1021 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
     1081 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
     1141 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
     1201 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
     1261 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
     1321 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
     1381 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
     1441 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
     1501 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
     1561 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
     1621 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
     1681 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
     1741 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
     1801 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
     1861 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
     1921 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
     1981 agacgatctt gttcgtcaaa agcaatgatc ccgacaccac caggatgaaa taccagttct
     2041 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
     2101 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
     2161 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
     2221 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
     2281 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
     2341 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
     2401 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
     2461 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
     2521 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
     2581 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
     2641 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
     2701 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
     2761 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
     2821 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
     2881 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
     2941 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
     3001 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
     3061 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
     3121 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
     3181 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
     3241 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
     3301 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
     3361 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
     3421 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
     3481 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
     3541 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
     3601 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
     3661 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
     3721 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
     3781 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
     3841 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
     3901 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
     3961 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
     4021 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
     4081 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
     4141 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
     4201 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
     4261 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
     4321 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
     4381 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
     4441 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
     4501 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
     4561 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
     4621 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
     4681 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
     4741 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
     4801 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
     4861 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
     4921 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
     4981 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
     5041 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
     5101 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
     5161 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
     5221 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
     5281 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
     5341 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
     5401 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
     5461 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
     5521 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
     5581 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
     5641 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
     5701 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
     5761 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
     5821 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
     5881 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
     5941 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
     6001 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
     6061 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
     6121 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
     6181 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
     6241 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
     6301 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
     6361 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
     6421 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
     6481 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
     6541 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
     6601 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
     6661 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
     6721 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
     6781 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
     6841 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
     6901 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
     6961 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
     7021 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
     7081 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
     7141 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
     7201 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
     7261 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
     7321 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
     7381 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
     7441 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
     7501 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
     7561 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
     7621 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
     7681 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
     7741 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
     7801 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
     7861 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
     7921 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
     7981 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
     8041 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
     8101 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
     8161 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
     8221 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
     8281 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
     8341 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
     8401 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
     8461 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
     8521 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
     8581 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
     8641 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
     8701 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
     8761 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
     8821 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
     8881 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
     8941 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
     9001 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
     9061 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
     9121 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
     9181 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
     9241 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
     9301 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
     9361 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
     9421 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
     9481 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
     9541 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
     9601 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
     9661 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
     9721 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
     9781 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
     9841 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
     9901 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
     9961 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    10021 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    10081 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    10141 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    10201 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    10261 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    10321 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    10381 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    10441 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    10501 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    10561 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    10621 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    10681 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    10741 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    10801 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    10861 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    10921 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    10981 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    11041 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    11101 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    11161 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    11221 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    11281 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    11341 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    11401 catttttttc tccccgttct ttttttatt
//
LOCUS       DAPCMU010000087        11181 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPCMU010000087 DAPCMU010000000
VERSION     DAPCMU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.090"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(25..162)
                     /locus_tag="RD744_001881"
     CDS             complement(25..162)
                     /locus_tag="RD744_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDU0557873.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="RD744_001882"
     CDS             complement(159..1343)
                     /locus_tag="RD744_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDU0557874.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="RD744_001883"
     CDS             complement(1599..1853)
                     /locus_tag="RD744_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557875.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="RD744_001884"
     CDS             complement(1878..3020)
                     /locus_tag="RD744_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDU0557876.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="RD744_001885"
     CDS             complement(3080..4432)
                     /locus_tag="RD744_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDU0557877.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="RD744_001886"
     CDS             complement(4503..4847)
                     /locus_tag="RD744_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDU0557878.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="RD744_001887"
     CDS             complement(4857..5231)
                     /locus_tag="RD744_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0557879.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="RD744_001888"
     CDS             complement(5244..5558)
                     /locus_tag="RD744_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDU0557880.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="RD744_001889"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="RD744_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="RD744_001890"
     CDS             complement(8976..9638)
                     /locus_tag="RD744_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557881.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="RD744_001891"
     CDS             10255..10485
                     /locus_tag="RD744_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557882.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="RD744_001892"
     CDS             10491..10790
                     /locus_tag="RD744_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557883.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(10826..>11181)
                     /locus_tag="RD744_001893"
     CDS             complement(10826..>11181)
                     /locus_tag="RD744_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557884.1"
                     /translation="YLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDEYLKENY
                     DIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNETITKTDM
                     ALMNREAEKIINKLT"
BASE COUNT         3104 a         2449 c         1878 g         3750 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgcttgaa attcattagg tgagtttgtt tattattttt tctgcttctc
    10861 gattcatcag cgccatatct gttttcgtaa tggtttcatt aggaatatag tcagcacatt
    10921 ttcttttctc taacattcta tacccactat tatgtattcc taaggtattc aattccttaa
    10981 ctgttgaaaa caaattccaa acggtttggt ggcttccttt tccgctactt ccattataaa
    11041 taatatcata attttctttc aaatactcat cagatttttt aaaagcactg tagtatgctc
    11101 ttgaaacagc agttcttcta taagattctt tagaattata tttatcatct gattcaattt
    11161 gtttggaaat agataaatat t
//
LOCUS       DAPCMU010000088        11118 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPCMU010000088 DAPCMU010000000
VERSION     DAPCMU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.091"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            80..1339
                     /gene="lysA"
                     /locus_tag="RD744_001894"
     CDS             80..1339
                     /gene="lysA"
                     /locus_tag="RD744_001894"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDU0557885.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1326..2126
                     /gene="dapF"
                     /locus_tag="RD744_001895"
     CDS             1326..2126
                     /gene="dapF"
                     /locus_tag="RD744_001895"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDU0557886.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2129..3010
                     /locus_tag="RD744_001896"
     CDS             2129..3010
                     /locus_tag="RD744_001896"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDU0557887.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3007..3723
                     /locus_tag="RD744_001897"
     CDS             3007..3723
                     /locus_tag="RD744_001897"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDU0557888.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3729..4424
                     /gene="dapD"
                     /locus_tag="RD744_001898"
     CDS             3729..4424
                     /gene="dapD"
                     /locus_tag="RD744_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDU0557889.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4440..5441
                     /locus_tag="RD744_001899"
     CDS             4440..5441
                     /locus_tag="RD744_001899"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDU0557890.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5438..6799
                     /locus_tag="RD744_001900"
     CDS             5438..6799
                     /locus_tag="RD744_001900"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDU0557891.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6759..7910
                     /locus_tag="RD744_001901"
     CDS             6759..7910
                     /locus_tag="RD744_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDU0557892.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7885..9054
                     /locus_tag="RD744_001902"
     CDS             7885..9054
                     /locus_tag="RD744_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0557893.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9229..10608
                     /locus_tag="RD744_001903"
     CDS             9229..10608
                     /locus_tag="RD744_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0557894.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10889..11118
                     /locus_tag="RD744_001904"
                     /pseudo
     CDS             10889..11118
                     /locus_tag="RD744_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3699 a         1946 c         2494 g         2979 t
ORIGIN      
        1 attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag
       61 tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt
      121 ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat
      181 gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc
      241 taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga
      301 tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat
      361 ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt
      421 gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg
      481 ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca
      541 tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga
      601 gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca
      661 tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt
      721 gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg
      781 gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat
      841 tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga
      901 accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa
      961 aaagacacca cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc
     1021 agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac
     1081 agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc
     1141 ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg
     1201 atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa
     1261 tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt
     1321 ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg
     1381 cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata
     1441 cagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg
     1501 gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc
     1561 agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta
     1621 cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc
     1681 cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg
     1741 gtgaaaaagt aacgatcact agtttatttt taggaacgat tcatacagtt gtttttgttg
     1801 aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt
     1861 tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc
     1921 gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt
     1981 atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac
     2041 aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg
     2101 aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac
     2161 gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca
     2221 tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct
     2281 cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt
     2341 gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca
     2401 ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag
     2461 tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat
     2521 tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt
     2581 agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat
     2641 ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat
     2701 cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc
     2761 taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga
     2821 aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc
     2881 tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt
     2941 ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt
     3001 tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt
     3061 gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg
     3121 cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag
     3181 aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat
     3241 acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt
     3301 gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg
     3361 ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca
     3421 tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta
     3481 tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca
     3541 ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc
     3601 aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg
     3661 ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg
     3721 taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc
     3781 ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg
     3841 gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag
     3901 ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc
     3961 ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc
     4021 aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag
     4081 taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg
     4141 gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg
     4201 ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag
     4261 gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg
     4321 cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga
     4381 caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa
     4441 tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca
     4501 aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt
     4561 cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa
     4621 cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt
     4681 ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg
     4741 aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc
     4801 ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac
     4861 aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg
     4921 ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc
     4981 cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata
     5041 caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc
     5101 cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag
     5161 ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag
     5221 ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag
     5281 gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt
     5341 tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg
     5401 ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc
     5461 gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt
     5521 tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga
     5581 tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc atcatgttga
     5641 ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc
     5701 cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa
     5761 gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga
     5821 gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa
     5881 aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat
     5941 ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg
     6001 gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa
     6061 ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat
     6121 catcgaggaa atctctttta gagaactgag agagatggct tatatggggg ctaacgttct
     6181 tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac
     6241 caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg
     6301 actgacagga atagctggac gcaaggattt tctttccatc actttattca aacgtcatat
     6361 gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc
     6421 aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat
     6481 tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat
     6541 cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat
     6601 tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt
     6661 atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac
     6721 agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg
     6781 cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg
     6841 aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg cgaaacagga ctactccttt
     6901 attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag
     6961 tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg
     7021 gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact aaacacattg
     7081 aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg
     7141 cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct
     7201 ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg
     7261 cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg
     7321 aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg
     7381 accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa
     7441 cgatccgcaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt
     7501 cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta
     7561 cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct
     7621 tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag
     7681 ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg
     7741 gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg
     7801 gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca
     7861 gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt
     7921 tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat
     7981 gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca
     8041 ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa
     8101 ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa
     8161 acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt
     8221 ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta
     8281 gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt
     8341 cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt
     8401 ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc
     8461 gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg
     8521 acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata
     8581 agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa
     8641 aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg
     8701 atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg
     8761 aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt
     8821 gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa
     8881 actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca
     8941 ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg
     9001 gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata
     9061 gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt
     9121 tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt
     9181 taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc
     9241 aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca
     9301 cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg
     9361 tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat
     9421 ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa
     9481 acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa
     9541 gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta
     9601 ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc
     9661 aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt
     9721 gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg
     9781 atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac
     9841 ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac
     9901 ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa
     9961 gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt
    10021 ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga
    10081 gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact
    10141 gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa
    10201 ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca
    10261 agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat
    10321 gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc
    10381 caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat
    10441 gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa
    10501 gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg
    10561 aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata
    10621 tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa
    10681 acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca
    10741 ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga
    10801 ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta
    10861 aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga
    10921 tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt
    10981 acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg
    11041 cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa
    11101 ttcttatcga ggaaaagt
//
LOCUS       DAPCMU010000089        11100 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPCMU010000089 DAPCMU010000000
VERSION     DAPCMU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.092"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            390..1829
                     /locus_tag="RD744_001905"
     CDS             390..1829
                     /locus_tag="RD744_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0557895.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2124..2342
                     /locus_tag="RD744_001906"
                     /pseudo
     CDS             2124..2342
                     /locus_tag="RD744_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2591..2806
                     /locus_tag="RD744_001907"
     CDS             2591..2806
                     /locus_tag="RD744_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557896.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3023..3279)
                     /locus_tag="RD744_001908"
                     /pseudo
     CDS             complement(3023..3279)
                     /locus_tag="RD744_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3466..4377)
                     /locus_tag="RD744_001909"
     CDS             complement(3466..4377)
                     /locus_tag="RD744_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0557897.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4396..5400)
                     /locus_tag="RD744_001910"
     CDS             complement(4396..5400)
                     /locus_tag="RD744_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDU0557898.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5397..7520)
                     /locus_tag="RD744_001911"
     CDS             complement(5397..7520)
                     /locus_tag="RD744_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0557899.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7525..9972)
                     /locus_tag="RD744_001912"
     CDS             complement(7525..9972)
                     /locus_tag="RD744_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0557900.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9959..10348)
                     /locus_tag="RD744_001913"
     CDS             complement(9959..10348)
                     /locus_tag="RD744_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0557901.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10408..10911)
                     /locus_tag="RD744_001914"
     CDS             complement(10408..10911)
                     /locus_tag="RD744_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDU0557902.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10924..11085)
                     /locus_tag="RD744_001915"
     CDS             complement(10924..11085)
                     /locus_tag="RD744_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557903.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         3238 a         2330 c         1998 g         3534 t
ORIGIN      
        1 tgatatttag tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt
       61 ggaaaacaaa taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa
      121 tttgcttacg tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt
      181 ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa
      241 ataaagcatc tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt
      301 cctatggcta gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc
      361 tacacgatca ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc
      421 aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg
      481 atggcagtat tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga
      541 atcaagcgga atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt
      601 ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct
      661 ttgtcttggg atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac
      721 gggtggtaca agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg
      781 aagtctttcc catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat
      841 taggctcgat tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt
      901 acattttttg gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct
      961 taccaaaaga tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg
     1021 cggctacgat tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc
     1081 atccagtccc ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag
     1141 ttgaaaagaa agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta
     1201 ccatgagctt gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga
     1261 ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga
     1321 tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg
     1381 ttggcccgga gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt
     1441 atatgttttt gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg
     1501 gtgccggcaa tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta
     1561 tcgaaaactt gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca
     1621 gcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc
     1681 aagtaactaa ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct
     1741 ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa
     1801 aacaaggagg tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc
     1861 tgcgtatggg tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc
     1921 gaaaaactca agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac
     1981 aagaagactg cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt
     2041 ttagaggcta ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac
     2101 ccagattatg taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat
     2161 ttccgtctag tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa
     2221 ttcgagaatg gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta
     2281 tcaatatttt gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt
     2341 aggaacaacg gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac
     2401 tcttttatca agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct
     2461 ttagcatttt tgatttaggt tccttttcta attataataa gatgaccact acagtaacct
     2521 actgaaatat ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat
     2581 cttcattctt atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga
     2641 ttttacttcc aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct
     2701 gaaaagtggt gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga
     2761 atttgctatg aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga
     2821 attacttgcc acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg
     2881 aatagagcat acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc
     2941 tatccccgta gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat
     3001 ttttgaccct tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt
     3061 taattcggtt ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca
     3121 ttcatctaag gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc
     3181 tgacattaca tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc
     3241 aagaagaaaa actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat
     3301 atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc
     3361 gagttaccga atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata
     3421 agttacacta cttataactt ctaataaaat tagattgagt cattattatt taacatcaac
     3481 gattttccag tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt
     3541 tgatacatta tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag
     3601 ataaactgga ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc
     3661 attttcaaca taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt
     3721 ttctaaaaaa tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac
     3781 ttgttttgct tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac
     3841 taaatgattc ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg
     3901 cgtctttttt ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga
     3961 gtgtttttca acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc
     4021 tcgaaccata tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact
     4081 cattctcttt tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc
     4141 ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc
     4201 atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca
     4261 agacaataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg
     4321 ttcttttttc ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct
     4381 ttttcctctt ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt
     4441 ttccgttaga ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc
     4501 gtaaatgccc acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa
     4561 atcacctggt ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc
     4621 cacccgagga agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc
     4681 aaaccccgtt tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc
     4741 ctcatcaaaa atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt
     4801 cactaataaa cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat
     4861 cgcgattggg ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc
     4921 agctaaagca aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc
     4981 atagttatag gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac
     5041 caaagacgca aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg
     5101 tggtaatccg gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac
     5161 ttgaataatc gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt
     5221 ttccaccatt gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt
     5281 gccagaacta ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat
     5341 acccataaaa gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat
     5401 tttttctcac ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg
     5461 gtctgctttg gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct
     5521 gattggcttt tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga
     5581 ttcagatttg ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga
     5641 ggttgagctg ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct
     5701 tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct
     5761 cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct
     5821 tttttcgact tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt
     5881 acctgatcct ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg
     5941 ccgatttttc tcccagccaa attcattcgg gattgtttct tattatttga aactggattc
     6001 tccttagtta agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga
     6061 gaagaaccgg atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact
     6121 gctgcaccac caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt
     6181 cgattcatta acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta
     6241 ctctggaggt tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc
     6301 acaatttgca gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc
     6361 gtggagatgc taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta
     6421 ttgaataaac gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat
     6481 aagaaactaa ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata
     6541 atccctgtta gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc
     6601 acggtcccta aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca
     6661 atctctgcct tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat
     6721 agaatcttgt tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt
     6781 agtaaccaag gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta
     6841 tcttttcctt ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct
     6901 tcactaacat ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg
     6961 aacgaacccg atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg
     7021 gtttctcgtt ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat
     7081 aatagaaacc caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat
     7141 gtttggatat tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc
     7201 tcttgaacca cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta
     7261 gtaatggcat aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc
     7321 aaccaatccc aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg
     7381 gaatatagat ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta
     7441 ccaactaaac tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc
     7501 cgaaaaagtt tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc
     7561 aaaggcatgg aagagttctt caaagactgg atgtacctga accacgccta cccgaccata
     7621 caaatcctgc atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc
     7681 atcttctcga tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact
     7741 gcgaaacgca aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact
     7801 attttgcgtg acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt
     7861 cgataatgtt ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat
     7921 tttgaagatt tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag
     7981 catggaaaca ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg
     8041 taaaaccaaa tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt
     8101 aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg
     8161 atccgcgata ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa
     8221 taatccccgt tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt
     8281 ctccccatct cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc
     8341 agaatccttc tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt
     8401 actagttaag ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc
     8461 accccgttcc ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata
     8521 aactaagaga ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc
     8581 tagcgcattg gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac
     8641 attgcgcccc gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg
     8701 agtggcgcca aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt
     8761 catgtatcgc ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg
     8821 ccggactagt tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt
     8881 tagttcatct actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc
     8941 tttggactga tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt
     9001 ggaatttgtt tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt
     9061 cttattgcga acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt
     9121 gtcaattggg aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc
     9181 catttccccc acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg
     9241 ttccatgcat aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc
     9301 gtaacgtttc accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc
     9361 ttcaaatggt gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg
     9421 tgtgactcgt ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt
     9481 ggtatatcgg cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt
     9541 cactccatag acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt
     9601 gacctcttca tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact
     9661 atcgccaatc atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc
     9721 tgttaatcgc ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc
     9781 tgtggcaatt tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa
     9841 gttatcgtgg acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa
     9901 ctcatagtag gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt
     9961 aatcgggtac atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct
    10021 gttctttgac tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag
    10081 atcgtaaaaa tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa
    10141 ctgccaccgg tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca
    10201 catgggcaaa tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag
    10261 tcacaggaaa aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa
    10321 tactggtgta gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta
    10381 gaaatcaact acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt
    10441 taccaaataa ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt
    10501 cttcaagtgt tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac
    10561 atcactcata tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc
    10621 ctcaaaagaa ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc
    10681 ttcaagttcc tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc
    10741 aatctcaaag ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc
    10801 aattcgttct ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc
    10861 accttcgtta tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc
    10921 cccttacgca ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc
    10981 attaaagacc aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa
    11041 ctcccgctta aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
//
LOCUS       DAPCMU010000090        10972 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPCMU010000090 DAPCMU010000000
VERSION     DAPCMU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.093"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            476..1339
                     /locus_tag="RD744_001916"
     CDS             476..1339
                     /locus_tag="RD744_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0557904.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="RD744_001917"
     CDS             complement(1468..3090)
                     /locus_tag="RD744_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0557905.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="RD744_001918"
     CDS             3339..3860
                     /locus_tag="RD744_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDU0557906.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="RD744_001919"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="RD744_001919"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDU0557907.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="RD744_001920"
     CDS             5941..6759
                     /locus_tag="RD744_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDU0557908.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="RD744_001921"
     CDS             complement(6862..7059)
                     /locus_tag="RD744_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557909.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="RD744_001922"
     CDS             7228..7806
                     /locus_tag="RD744_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDU0557910.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="RD744_001923"
     CDS             7901..8038
                     /locus_tag="RD744_001923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557911.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="RD744_001924"
     CDS             complement(8851..9354)
                     /locus_tag="RD744_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDU0557912.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="RD744_001925"
     CDS             9713..10738
                     /locus_tag="RD744_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0557913.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         3362 a         1903 c         2127 g         3580 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tt
//
LOCUS       DAPCMU010000091        10882 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPCMU010000091 DAPCMU010000000
VERSION     DAPCMU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.094"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..440)
                     /locus_tag="RD744_001926"
     CDS             complement(<1..440)
                     /locus_tag="RD744_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDU0557914.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQS"
     gene            complement(676..2184)
                     /locus_tag="RD744_001927"
     CDS             complement(676..2184)
                     /locus_tag="RD744_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDU0557915.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            2343..2534
                     /locus_tag="RD744_001928"
     CDS             2343..2534
                     /locus_tag="RD744_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557916.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2737..3654
                     /locus_tag="RD744_001929"
     CDS             2737..3654
                     /locus_tag="RD744_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDU0557917.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3647..4720
                     /locus_tag="RD744_001930"
     CDS             3647..4720
                     /locus_tag="RD744_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557918.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4868..5386
                     /locus_tag="RD744_001931"
     CDS             4868..5386
                     /locus_tag="RD744_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557919.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            5367..5759
                     /locus_tag="RD744_001932"
     CDS             5367..5759
                     /locus_tag="RD744_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDU0557920.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5835..6770
                     /locus_tag="RD744_001933"
     CDS             5835..6770
                     /locus_tag="RD744_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDU0557921.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6903..7682
                     /locus_tag="RD744_001934"
     CDS             6903..7682
                     /locus_tag="RD744_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDU0557922.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7675..7992
                     /locus_tag="RD744_001935"
     CDS             7675..7992
                     /locus_tag="RD744_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDU0557923.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            8236..9846
                     /locus_tag="RD744_001936"
     CDS             8236..9846
                     /locus_tag="RD744_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0557924.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            10003..10851
                     /locus_tag="RD744_001937"
     CDS             10003..10851
                     /locus_tag="RD744_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0557925.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3475 a         1913 c         2154 g         3340 t
ORIGIN      
        1 gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc catcccgttg
       61 attttactta atcctagacc aacagctttc ataaataatg gaagaatctt gatatagcgt
      121 agaataccga taatagcaga aataaataca attggcatca atacggaaag aaagaaaggt
      181 gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc agcaaaagca
      241 agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt
      301 aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg tttcaatttg
      361 attgcttttt tgttactgct gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata
      421 atacctaata aataactcaa ataatcactc ctattccaaa ttccgtttta ttttacctgt
      481 tttgcaaata aaatactaat ctttagtgaa attttcatta cttttttaga gtgaaacgca
      541 ttcattttcc attagaaaat aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt
      601 agtaaagtct ataaaaaaac gaaactatcg attaagctaa tagtttcgtt ttcaacattt
      661 ataagtaaac tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt
      721 aatatcgatt cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga
      781 gtgggcttgt gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt
      841 cgttactgca ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga
      901 ggaatgaagt gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa
      961 aggaatgtca tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga
     1021 cgcatccatc aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc
     1081 aatcacagca gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg
     1141 ggcagggact accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt
     1201 gattcgatta actgcttctt cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa
     1261 ataacgacgg atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc
     1321 aaatcctaca cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt
     1381 aattttttca ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac
     1441 agctacttta ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc
     1501 gactgtggct gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg
     1561 gaaagtttcg aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg
     1621 gttcaagcaa gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg
     1681 tttagttgtt tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac
     1741 gttgactgga tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag
     1801 tttattgata aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt
     1861 gattaataag ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt
     1921 aatcccataa tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag
     1981 tatttcagct tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc
     2041 tggaagtaca cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa
     2101 tttgtcatag tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc
     2161 tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat
     2221 ttttttgagg attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt
     2281 taaaccaact tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct
     2341 acatgaaaaa aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta
     2401 ccggttatga aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa
     2461 acgaagaaac gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc
     2521 tgcctagtaa ataaaaaata agataaaggt catcggattc gattctgtcc tatcagatgg
     2581 aaagactagt tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg ttttatggag
     2641 cattgcccgc tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat
     2701 aagtgaaaaa cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca
     2761 aattatgagc cagacgaact agaggagacg aacgcggatc aggatacagg acaagaatcg
     2821 tcttcttttt acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac
     2881 ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac
     2941 aatggccgat tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa
     3001 ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta
     3061 gaaaacatct cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc
     3121 acggtcggta ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag
     3181 tgggagaaat attcccaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac
     3241 gcaaaggtga tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc
     3301 aatcgacaaa tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag
     3361 gaagaaattc aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg
     3421 cttccttata ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa
     3481 gcaagagcag accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc
     3541 tatttcggta gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt
     3601 ggcaatgaga atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga
     3661 tgaagcagaa tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc
     3721 attggaagaa caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga
     3781 aacaaaaata catgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac
     3841 ccatgcttac gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca
     3901 agatatcgtc agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa
     3961 agatttgatc gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa
     4021 tgaagaacca atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt
     4081 actgaaaccg ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa
     4141 aacaagacat ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt
     4201 tgtattaccg accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc
     4261 accagagaca atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg
     4321 gtcagtggat cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca
     4381 attattagcc catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac
     4441 gaagccactt cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga
     4501 tatttttatc gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac
     4561 ttatcaagta cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca
     4621 aacagtcttg tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa
     4681 ttacgaattc ttggtcccat taatggtgag aaagtcctga ttggataagt tggattacca
     4741 gatgtcattc agataattct gttccaaagg acttgcggca gttatccaga ataattgtca
     4801 caagtctttt ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc
     4861 attaggaatg tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc
     4921 aggtaggatc gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt
     4981 tatagtactt tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat
     5041 cagcagtttt tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt
     5101 tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt
     5161 aaagggagtc tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga
     5221 tacgctgtta tatcgtttgc ctcaaccttg gatctattac cttcaagggg tgtcgtttga
     5281 tttgatgcac agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg
     5341 tttttacaat cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga
     5401 gtgtcagtat actcattttg acaagttgca gtacaaccta tacacaatcg gcttccaacc
     5461 aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa
     5521 acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt attttgaaac
     5581 gaaattttga agtcaaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg
     5641 atgagaaaaa tcaactctat tggatgttta ctgtggacgg gaaaagtgct actactggtg
     5701 ccaaagagat caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag
     5761 tttcataaaa aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa
     5821 agaaggaaaa taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc
     5881 taggggctaa tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg
     5941 ccgccatgct aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt
     6001 tattcggtga taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac
     6061 gagcaaaata ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac
     6121 ttggggtgat ggaagctttt gaaaagttga cgcatccatc ccatgaagtg ggtaatccga
     6181 tcattgttat agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg
     6241 caatgaaaga aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg
     6301 aaagtcgaca cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt
     6361 tggtcttggc tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg
     6421 cgatcagcgg attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag
     6481 aattttatag tctgatcatc ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt
     6541 tagcttttga acgtcctgag atcagtcggc tgatcgatat aaaaacagtt catttaagtc
     6601 caacggagat tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt
     6661 actcggtcat caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt
     6721 atatatacat tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt
     6781 catgaattga agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat
     6841 ttagcgaatt tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt
     6901 taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt
     6961 gtataagcta tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg
     7021 atcctctctt atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag
     7081 ttgcatctat gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt
     7141 tagaattacg ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc
     7201 ggacgttttt ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact
     7261 taaagttttc aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct
     7321 caatgactgc atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag
     7381 tagatgctga ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc
     7441 agatgaaaag cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat
     7501 tcatggtatt attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg
     7561 ccggatttat gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg
     7621 gtaaagatgt caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact
     7681 aaactatatt tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg
     7741 ttcccgatgc tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa
     7801 tatgtaccgg tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa
     7861 catctggata ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct
     7921 tacaaaacac gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg
     7981 tctatgcttt aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa
     8041 ctgatctatc cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga
     8101 taaaaaagat tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg
     8161 gatttgtcct tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata
     8221 gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct
     8281 aatcttctat gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg
     8341 aacaatcaag atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga
     8401 ttgttcctga tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta
     8461 gccacaaaag aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc
     8521 atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc
     8581 ggaatcagtc cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca
     8641 tctttgattg ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat
     8701 ctgcggcctt atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg
     8761 gctggtactt attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc
     8821 agtacagtcg cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga
     8881 cgcagaaaag acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga
     8941 gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc
     9001 tcagtcatta ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt
     9061 ttccgtccag agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat
     9121 ccaaataaat tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg
     9181 ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat
     9241 agtttttcga ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct
     9301 ggaccactat acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg
     9361 gctttgctag cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct
     9421 ttgaatcacc atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc
     9481 ttccaagtaa tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta
     9541 gcattcggta ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca
     9601 cctttagcaa agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt
     9661 gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc
     9721 tatgcaatga tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct
     9781 caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa
     9841 gaataggtta actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg
     9901 cttgtcgttt ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt
     9961 ataaggtctg gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt
    10021 acagatagtt cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt
    10081 ccaatccctt tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat
    10141 gaatattatg gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta
    10201 ggggaagttt tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt
    10261 atccatcttt cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac
    10321 atcagcggag taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac
    10381 atggtagaag ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca
    10441 catatcgatc gaatccgaga taatacctat gcttatctga ttgtagatga tttgaacaat
    10501 ttagtacgag gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc
    10561 aaaccgatcc tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt
    10621 aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg
    10681 ccagtcaaac tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag
    10741 aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt
    10801 ggtacacatt taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta
    10861 ttcaaaaaac ataccaaaaa ta
//
LOCUS       DAPCMU010000092        10790 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPCMU010000092 DAPCMU010000000
VERSION     DAPCMU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.095"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(8..1813)
                     /gene="glmS"
                     /locus_tag="RD744_001938"
     CDS             complement(8..1813)
                     /gene="glmS"
                     /locus_tag="RD744_001938"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDU0557926.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2222..2512
                     /locus_tag="RD744_001939"
     CDS             2222..2512
                     /locus_tag="RD744_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557927.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2525..3550
                     /gene="sppA"
                     /locus_tag="RD744_001940"
     CDS             2525..3550
                     /gene="sppA"
                     /locus_tag="RD744_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDU0557928.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3553..4212
                     /locus_tag="RD744_001941"
     CDS             3553..4212
                     /locus_tag="RD744_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDU0557929.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4369..5301
                     /locus_tag="RD744_001942"
     CDS             4369..5301
                     /locus_tag="RD744_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557930.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5455..6744
                     /locus_tag="RD744_001943"
     CDS             5455..6744
                     /locus_tag="RD744_001943"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDU0557931.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6991..8241
                     /gene="clpX"
                     /locus_tag="RD744_001944"
     CDS             6991..8241
                     /gene="clpX"
                     /locus_tag="RD744_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDU0557932.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8324..8911
                     /locus_tag="RD744_001945"
     CDS             8324..8911
                     /locus_tag="RD744_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDU0557933.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8985..9140)
                     /locus_tag="RD744_001946"
     CDS             complement(8985..9140)
                     /locus_tag="RD744_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557934.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            9276..10604
                     /locus_tag="RD744_001947"
     CDS             9276..10604
                     /locus_tag="RD744_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557935.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            10615..>10790
                     /locus_tag="RD744_001948"
     CDS             10615..>10790
                     /locus_tag="RD744_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557936.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVY"
BASE COUNT         3569 a         1869 c         2232 g         3120 t
ORIGIN      
        1 tcttttatta ttctactgtc actgatttcg ctaaattacg tggtttgtca acgtcgaatc
       61 cccgtaatag cgttgcgtaa tatgcaagta attgagtcgg tacgactgtg gttagagtag
      121 ttagataagg gtggacagtt ggtagcacca gctggtcact ttccttttct aatccctctg
      181 agacaataac tagtgtttta gcgccacggc tttcgacttc tttcaggttt cctcgagtat
      241 gcgcaccagt cacttcttca ctgatgattc cgatcactgg tgttccttct tcgatcaaag
      301 caatcgttcc atgtttcaat tcgccagcgg caaaaccttc tgcctggata taactgattt
      361 ctttcaattt caaagcagct tcaagagaaa cgttgtagtc agcaccacgt ccaatgtaaa
      421 aggcatttct tgtatcgctg agatatactt ctgccaactc ttctaaataa tctttttggg
      481 cgatgaccga ttccatcgca ttagcaacta aactcaattc atgagtgata tcgaatacaa
      541 gagaagtttc aaattctttt ttatcaccga tagctttggc taataaagtt agtacagcta
      601 tttgtgcagt ataagctttc gttgatgcta ctgcgatttc gggtccagca tgcaatagca
      661 gtgtaaagga tgcttcccgc gaaagagtag aaccagcaac atttgtaatc gtaagtgaag
      721 ggtagccgag acggttgatt tttaccagca cttggcggct gtctgctgtt tcgccacttt
      781 gactcaagaa aataaagaat ggtttttttg aaagaagagg tgtgttatag ccaaattcac
      841 tggctaaatg aacctcaaca ggaatctgtg tcaatttttc caaagtctgt ttacctgcta
      901 accccgcatg gtaactagtt ccgcaagcaa cgatatagat tcgatcgcta tcagacaatg
      961 tatcgaccag cttttgatca agagagacac gattgtcttc tgttacgtat ttttgagcga
     1021 tacgacgcat gataataggc tgttcgtcga tttctttcag catataaaaa ggataggtac
     1081 ctttctcgat atctgaagca tctacttgtg cttcgaatga agaacgttct acgatgtcac
     1141 ctgcaaaatt ttgaataata actttttctg atgtgacggt aaccatctct tcatcttcaa
     1201 tctcaacgaa ttccttcgta tgagcaagca tagccattgc atcgctggca atcacattga
     1261 atccatcgcc gagtccgatt aatagtggac ttttattttt agcgacgtaa ataacatcag
     1321 gtgctgtccg atcaatcaaa gcgaaagcgt aagaaccttt gatgacccgc aaagctttta
     1381 aaaacgcctc ttgcgttgta gtatccttcg cgaaagtttc aattagatga gcgataatct
     1441 cagtatcggt ctcaccaata aagtggtcat ttgctaaata agcaatttta agttcttcaa
     1501 agttttcgat tacgccgttg tgaacgagca cgaagcgtcc actttgggaa gtatgtggat
     1561 gggcgttttc ttcagaaggt tgcccgtgag ttgcccatcg tgtatgacca atacctatat
     1621 ttcctgtagt atcaatattt attttgtttt tcaaattttg aattctgcct tgtgctttga
     1681 ccagataatc agttgtgcca tctgagacaa agataccagc tgaatcataa ccacggtatt
     1741 ctaatttttc tagaccgtca atcaatcctg gtgttacatt ttttaatcca atcattccaa
     1801 caattccgca cataaaaaac atcctacttt ctataatgaa ttcgtatgaa tgcttccagc
     1861 tgcaggaaaa tccttttgtt ttatctttgc agacattttt tgtggatttt ctgtagagtg
     1921 cagtccctag ggccatccgc cgaatctttc gataaaccct ctcctcgtca tctgtttaca
     1981 cagacctggc gctatcctaa aaggtataac tatcctcctt tttgtttttt atctggaaac
     2041 atttaaaatc ataacctttt gcgaatgttc ttgtcaacaa aaaaagaata aaaaataaat
     2101 tggtatatat caaattcttt gtcaaatgtc ctttttatgt caaaaaataa tgggttttta
     2161 tctatcatct ttttcaggag ttcggtacaa tagaaagtaa tcggatagag gaggagtaaa
     2221 aatggattca aaaattgaga tcatgacact gggaatgtta aaaaaacaat tgtccaaatt
     2281 cgaagcatca gctggtgtat cagatgatac gaaaatattt ttagatactg gctgggatag
     2341 catccaagaa atcgcacctg atgccttgga agttgctcaa gctcgtgagt ttacggtaga
     2401 agatgaatta acaaaagagt cgttttccgg gtatgcgcgg gaagaaaaag cagagcggtt
     2461 cgacacttct gagcagtcag aaactgtgat cgtcataaaa aacttgtatt agaaaggaga
     2521 aagaatgaat aaaagacgtt gggcagcagt tctgattgct gcaggattat ttgtggtttc
     2581 gttagtttca gctagtttga ctaagcctca ggaaaacgat caaattgagg gattgaacag
     2641 ttggttatat ggggatgaag aactagctcc aaatgtgttg gaagagggag acagtacgca
     2701 aaaaatcgta aaattatcag tagatggaac gattgctgcg ggagggtctt caggtttatt
     2761 tgcttctgaa ggttacaatc ataaaaattt tatggaacaa ctgaaggcga ttgaagaaga
     2821 tccaacagtc aaaggtatct ttttagaagt aaattctcct ggtggcggtg tctacgaaag
     2881 tgcggaaatc gctaagaaat tggatacgat tcgtaaagaa catgatatcc caatgtatgt
     2941 cagcatgaaa aacatggcag ctagcggtgg ctactatatt tctgctcaag cagataaaat
     3001 ctttgcaaca gaagaaacag tgaccggctc aattggtgtg atcatgtcag gattgaatta
     3061 ttcaggactt ctagaaaaac tgggtgtgga agatacaaca gttaaaagcg gagcgttgaa
     3121 agatatgggt tcaggaacgc gaccagagac aaaagaagaa gaagcggtgc ttcaagcata
     3181 tatcgacagc gcatatagtc gttttgtagc aatcgtcagc gagggacgga ataaatctga
     3241 agaagaagtc aaaaaaattg ctgatggaag gatttatgat ggtgtccaag caaaagaagt
     3301 aggtttagtc gatgaattgg gatttcctga agatgcttta aaagctatac gaaaggaaca
     3361 acagcttgaa gatgcggaac tagtagaata ttcttccacg ccatctgggt ttgctagtac
     3421 atggttagga agtaaactag cagaattcca gggattgaag ccttctgaga ctagtcagat
     3481 cttatccgtt cttgagaatc tagggacagc ggaatcacca aaagcaatgt attattacgg
     3541 aggtgagtaa gcatgtcaga gctttcaaat gaaaataagg aacaagaaaa agataagcaa
     3601 cagcagcctg ctgtaggacc tgactccaac ttttcccaaa aggtactgag ctcttttgaa
     3661 agagagcctt tgacgcccga agaaatcaaa aagaaacaac gtaaatggaa aaagtatacg
     3721 gacacaagaa atgaaccgcc aaaagcaaat gattttccta agtatttcta tgcaggtttt
     3781 tttatccgaa tgtttgcctt cttggtcgat cttttgtgta tcggagctat tacgcgttta
     3841 acacttggta ttgccaaaaa tctaggttgg cttaattttt cagattctta tctgagtctc
     3901 tacggattca cagccttact tatttatcta gcgtatttta ttttattgac gaaattcaat
     3961 catggccaaa cgattgggaa aatgatcttt ggtattcgag tcatcagctt tgatgaagaa
     4021 gaacttagct ggtcaaccgt tttagttaga gaaggtgctt gccggttcat tctaaaattt
     4081 cctttgctga tgttagggta tcttccagca gtctttaatc agaaaaagca acatctgggc
     4141 gattttttca ctaatacaag tgttgttacg atcaatctga tcaaagcgtt caaccaggaa
     4201 gtcaacagct gatgagcaat aaaaagcagt aacagttgaa agcaccttct aaaagtcaga
     4261 tctaaaatct agcttttaga gggtgcttca gttattttta attgtcatgc tttttttgtt
     4321 ttgctactat tgaacatgag ctgaatatga gcaagaggag gacactgcgt gaagccgatt
     4381 gattttccag aaaaatatga gaatttaatg cgagtggcac aacaagcact agcaaaccag
     4441 caatatcagc aggcaaaaga attatttcaa cgagcttacg agttgaaaga aagctttgag
     4501 gctaatagcc tgcttgtttt ttgtctttac gagttggacg agaaaaaaga agcattgaag
     4561 caagcactct tgcatgaaaa acagtatctt caaaatgagg agtttgctga attttatttt
     4621 gacttgttaa tcagtacaca agatttttta tatgctagaa aattgatagc aagtacagat
     4681 ttttatgaag ctttcgagca gcgggtcatt gaaaaaatcc aatttgcaga agaattatcc
     4741 ggacaaatgg agaggcagaa gatcaaggca ctccacaata aatcggagga acttccttcg
     4801 ttggaacctg cacgtcagct tgcgtctatt gatgagatcg aacagcttcc ttatcatgag
     4861 tttatccaaa cagcgagtaa acttattgta tcgccagagg tccatatttt agcaagggca
     4921 aaattactgg agacacttag gcaattgaac gaatgccatc ctgttttcta tttgacgatt
     4981 gaagaaaagt tagtcaaagt cataccaaaa gatctgccaa agccgcagca gcaggcaagt
     5041 taccagcaat tatgtgtatt ttcagatcgt tacgggaatg aagatgcgct gcttagttcc
     5101 gtgttaaaag aagaattcac gttgcaatct gcaattgttt atcctgtcta tgatacatac
     5161 atcgaagacc cgaagcgctg gtttcaactg actgtagagg cgtgtacagg aaaaacgatg
     5221 tatgaaggta cagaagatga aaaacaagat tttttaaaaa aaagagaaaa aattttgcag
     5281 caaatgatct tcttccatta gattttatca gtgaaaaaga taaaaatgtt atgaaaaaca
     5341 aggattctat ccattcaata tgtttacaat ttgaaagggt tagtgtacta tttaaaggta
     5401 tgcatgaaac gtttttgatg tataaaatga atttctaaac tcggagggaa ttaaatgact
     5461 gcaacatttg aaaaaaaagg cacgaatgat ggtgttttaa aatttacgat tgcgcaagac
     5521 gaaatccaaa aaggattaac aactgcgttt aataaagtga aaaaaagctt gaacgtacct
     5581 ggattccgta aagggaaagt ttctcgtcaa gtattcaacc gtatgtacgg tgaagaagct
     5641 ttatatgaag atgcattgaa tgctgtatta ccagaagctt atgaagctgc tgtcaaagaa
     5701 gctggcatcg atcctgtttc tcaaccaaaa atcgatgttg aaagcatgga aaaaggtcaa
     5761 gactgggtaa tcactgctga agtaacagtg aaaccagaag taaaattagg acaatataaa
     5821 gatctagttg ttgaaaaaca agaccgtgaa gtaacagatg ctgacgtgga tgcacgtatc
     5881 aaacgtgaac aagaagcaca agctgaatta gttatcaaag aagatgctgc agctgaaaat
     5941 ggcgatacag tagtaatcga cttcgaaggc tttgtagatg gcgaagcgtt tgatggcgga
     6001 aaaggcgaaa attactcact tgaattaggt tcaggctcat tcattccagg ttttgaagat
     6061 caattagttg gtcataaatc aggtgaaagt ctagatgtaa atgtaacttt cccagaagat
     6121 tatcaagcag aagatcttgc tggaaaagaa gcagtcttca aagtgacaat ccatgaagta
     6181 aaagcaaaag aagtgcctga attagatgac gaattcgcaa aagatgttga tgacgaagta
     6241 tcaacattag acgaattgaa agaaaaatat cgcaaagaat tagtagaaac aaaagaaaaa
     6301 gcagcggacg atgcaaaaga tgaagcagcg atccgtatgg ctgttgaaaa cgcagaaatc
     6361 gttgaattgc cacacgtgat ggttcatgat gaagtacatc gttcaatgga cgaattcttg
     6421 aacaacatgc aacgtcaagg tatctctcct gaaatgtact atcaattaac tggttctaca
     6481 gaagaagact tgcataaaca attcgaaggt gaagcagaaa tgcgcacaag aacaaatctt
     6541 gtgatcgaag caattgcagc tgctgaaaat cttcaagcaa cagaagaaga aatcaatgca
     6601 gaaatccaag aattatcaga aacatacaac atgccaatcg aacaaatcaa acgcgtgttg
     6661 acagaagata tgctggaaca tgatgtgaca atgaaaaaag caatcgaatt gatcacaggt
     6721 acagcgacag aagcgaaaga atagaaatca agtaataaat catttctgaa gaagctactt
     6781 ctagaaaatt gtttattcga aataagggac tgtgacacga ttttgtcaca gttccttatt
     6841 ttttgttaag agtaaaaaat gcaaaggtat aggtcagttg atcctttgtt aaacactttt
     6901 tctttttact aaactatgtt atgatagtaa aagactgtaa agtctttgat agtttcttgc
     6961 atactttttg caagttaagg ggtgaagacc atgtatgaca atccaagtag caatgagacc
     7021 gtacgctgtt ctttttgcgg gaaaacccaa gaagaagtga aaaagatcgt agctggtcca
     7081 ggggtctaca tctgtaatga atgtatcgac ctatgtaaag aaatcattga tgaagaattt
     7141 tatgaagaag ctgtacgcga attcactgaa gtccctaaac cactagaaat tcttgaagta
     7201 tttaataact acgtgattgg acaagatcgc gcaaaacgct cgttagctgt tgccgtatac
     7261 aatcactata aacgtgtcaa tcagcaagca caagaaaaca cagatgatgt tgaactgcaa
     7321 aaaagcaata tctgcttgat cggacctaca gggtctggta aaacattttt agcacaaaca
     7381 ttagcaaaaa cattgaatgt accttttgca atcgcagatg cgacaagttt gacagaagct
     7441 ggttatgtgg gggaagatgt agaaaacatt ttgcttaaat tgcttcaatc tgctgattac
     7501 aatgtggaac ttgcagaaaa agggatcatc tatattgatg agatcgataa aattgcgcgt
     7561 aaaagcgaaa atgtatctat tactcgtgac gtttccggcg aaggtgtcca acaagccttg
     7621 ctgaaaattc tagaaggaac tgttgcaagt gtgccacctc agggcggacg taaacatcca
     7681 catcaagaat ttattcagat tgatacgacg aacatattgt tcatcgtagg tggtgcattt
     7741 gatggcatcg aaacgattgt gaagaatcgt atgggcgaaa agacgattgg atttggtaca
     7801 caaaaccaaa aactttctga agacgaaagt gtcatgcagc atatcattcc tgaagattta
     7861 ctaaaattcg gattgatccc tgagtttatc ggacgattac ctgttatggc agctttagag
     7921 aaattgacaa cagatgatct ggtacggatc ttgactgagc cgaaaaatgc gctagtcaaa
     7981 caatatcaaa aacttctttc tctagataat accgaacttg aattcgaacc tgaagcgttg
     8041 agagccattg cgaaaaaagc aatcgaacgc aataccggtg ctcgaggact tcgttccatc
     8101 attgaagaaa tcatgatgga tgtgatgttt gatatccctt caaatgaaaa catcgaaaaa
     8161 gtgattatca caaaagaagc agctgaattg tctggtgagc caacagtaat ctataaagat
     8221 tcagagaaaa aagcgggttg atgaaaagag gctgccgtaa aagtcatttt ggcttttgcg
     8281 acagccgctt gctttattca tcaccttgtt aaggagagag accatgaaag tccatcaagc
     8341 tgaaattatc ataagtgcag tggcaccaaa acaatatcca gatacacaat tgccagaaat
     8401 agctctggca ggacgatcaa atgtagggaa atcttctttt atcaacacat tgattgatcg
     8461 aaagaattta gcccgtactt ccggtaagcc aggaaagacc cagacattga atttttattt
     8521 gatcgagaac gcattacatt ttgtcgatgt tccgggatat gggtatgcaa aagtatccaa
     8581 aactgagcga gcaaaatggg gaaagatgat cgagacatat ttaacgacta gagaccagct
     8641 acgtgcggtc gtttctttag tagatttgcg tcataagcct tctcaagaag atatccagat
     8701 gtatcaattt ttgaagtatt atgatatccc tgtaattgtt gttgccacta aggcagataa
     8761 gatccctcgt ggtaaatgga acaagcatga gagcgaaatc aaaaaagcgt tggattttga
     8821 tcgcaatgac acgtttattc tcttttcttc cgttacaaaa cagggaaaag aagaagcgtg
     8881 ggcagcaatc gaacaagcaa ttgaatcata aaaagaataa aaaagactta ccagtcaaac
     8941 tctttcataa aagtttggct ggtaagtctt tttttgagct taacttactt ttgtttcttg
     9001 tcttttttct tttcattttg tggctgtggt tttagaaatc gatcgatctc ttcttttggt
     9061 gcaactgttt tttccggatc gacagcaaat gattcgaaaa gtttatctaa attatctgta
     9121 cgttcaaatt tcaaccccat tgtaaacacc tctttgtttc tttttactta gtctatcatg
     9181 taatcgtcca aattggtttc taagacataa agttttagtt tttctttatc attggttata
     9241 attaaattat aaaaagattt gaaagaggtg agaaaatgat tggcgaagat aaattattga
     9301 tgactatcca gctcacagac ttaaatgaag aagaaaaaaa acaggaaata agcgattggg
     9361 ctattttgac acgtctgttg atccagccaa catttatccg ctctttcaca caacaagcgg
     9421 atccgtatga tcttcgaaag cttcaagaaa aaataatgct agcatcggta agagacgaaa
     9481 gttggttagt tgtaggaaat gacgagaacg aatgtagttt tcgcttggaa gataatcaat
     9541 tattgataaa aaatgtattg tctatctctg ttttcaaaga aaagcaattt ttgattcgag
     9601 acttcattca gaaaaaaatg attgctcatg gggtgttcgc ctatatgcga gcttatagcg
     9661 agtttatcta tcacaataca aaacagatct ctcaacggct attgtttgag aaaaaggatg
     9721 aaatcgaaca tttacctaaa atgaaacaac aaaacgggga agtagtagtc gattgcaacc
     9781 aatttccagg atacgatctt ttttaccaag gtttatgttt cacttcgtgt tgggaaatgt
     9841 attatagtcg ctattatcat caaatcatcc ctaaaccgat ttttctcgac atccaacaag
     9901 tagaaaaagt gaaggaatta gaaaacgaag tcatatgtat ccagctttat cgagacccct
     9961 ttaactggca aaagccaaac aatcaaatgt ttcaatccta ttttagggac cagcttgggt
    10021 ttgatcatct tgcttgggac aatggtgtcg gtttattgaa accgccattc gttgaataca
    10081 tttacactga ccatacgatc caatctgtcc agtatcagaa tgcacaaatg caacctgttc
    10141 ctaaaaagaa tgcttcattt tttgtaacaa aaagttatga tatccaacaa ggagattata
    10201 aagaaaggcg tgttcgcgga acactaaatg ctcaggcgta ttttccttgg gttgacgaga
    10261 atcgatcacg catgatgtgc tataaagtta tcgatccgac tgttgctttg gataatggaa
    10321 tcgaagctta ttgttattat attagggaat atctagaagt agaggtaaca gatgaaaaat
    10381 atcaagagta tcttttatcc ttaagaatct atgttccttc agaatccctg tcagaattac
    10441 cgttaaagga aatcaaacat agactctctg atgttacctt caaacgaata aagaaaaagc
    10501 ggcgaagtat ccaattcgat gtcaagaaag gtgagcagca tctgcgtgtg caatttttag
    10561 attaccgaga attagaacaa ttgattactt tacaaaaaat atgaggtgaa gaaaatggat
    10621 aaacaactct attatcgaat aatggttccg gcatcattat tatccgtctg tctctttttt
    10681 gttcctaatt ttcctattcg atcagtcatc ggttttttgg tcatgtttgg cgggatggga
    10741 ctttattatt atcttcttca taaggaaaga aaacctgctt ctgtttatat
//
LOCUS       DAPCMU010000093        10738 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPCMU010000093 DAPCMU010000000
VERSION     DAPCMU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.096"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            296..565
                     /gene="rpsN"
                     /locus_tag="RD744_001949"
     CDS             296..565
                     /gene="rpsN"
                     /locus_tag="RD744_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDU0557937.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            580..759
                     /gene="rpmF"
                     /locus_tag="RD744_001950"
     CDS             580..759
                     /gene="rpmF"
                     /locus_tag="RD744_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDU0557938.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            791..1168
                     /locus_tag="RD744_001951"
     CDS             791..1168
                     /locus_tag="RD744_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557939.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1189..1338
                     /gene="rpmG"
                     /locus_tag="RD744_001952"
     CDS             1189..1338
                     /gene="rpmG"
                     /locus_tag="RD744_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDU0557940.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1362..2345
                     /locus_tag="RD744_001953"
     CDS             1362..2345
                     /locus_tag="RD744_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDU0557941.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2375..2497
                     /locus_tag="RD744_001954"
     CDS             2375..2497
                     /locus_tag="RD744_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDU0557942.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2515..4038
                     /locus_tag="RD744_001955"
     CDS             2515..4038
                     /locus_tag="RD744_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDU0557943.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4062..4301
                     /locus_tag="RD744_001956"
     CDS             4062..4301
                     /locus_tag="RD744_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557944.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4494..4955)
                     /locus_tag="RD744_001957"
     CDS             complement(4494..4955)
                     /locus_tag="RD744_001957"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0557945.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5150..5368)
                     /locus_tag="RD744_001958"
     CDS             complement(5150..5368)
                     /locus_tag="RD744_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557946.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5486..7102
                     /locus_tag="RD744_001959"
     CDS             5486..7102
                     /locus_tag="RD744_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDU0557947.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7343..8683)
                     /locus_tag="RD744_001960"
                     /pseudo
     CDS             complement(7343..8683)
                     /locus_tag="RD744_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8959..9114)
                     /locus_tag="RD744_001961"
     CDS             complement(8959..9114)
                     /locus_tag="RD744_001961"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDU0557948.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9410..9742)
                     /locus_tag="RD744_001962"
     CDS             complement(9410..9742)
                     /locus_tag="RD744_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0557949.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10256..10447)
                     /locus_tag="RD744_001963"
     CDS             complement(10256..10447)
                     /locus_tag="RD744_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0557950.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10541..>10738)
                     /locus_tag="RD744_001964"
     CDS             complement(10541..>10738)
                     /locus_tag="RD744_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0557951.1"
                     /translation="VTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGI
                     TRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3561 a         1706 c         2088 g         3383 t
ORIGIN      
        1 tttcatattg ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg
       61 gactaataaa agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt
      121 tgattatctc tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt
      181 atttcatgtt atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa
      241 cgtaacgatt acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc
      301 aaaaaagtcg aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga
      361 taaacgtcag gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga
      421 ttcaaatccg aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat
      481 gcgaaagttc ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc
      541 tggagtaaaa aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa
      601 acatctaagg cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt
      661 tcatttgacg aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac
      721 tataatggaa agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga
      781 gggaatcaaa atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct
      841 tgaaacgacc cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg
      901 aattattact gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga
      961 ttactatgag gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga
     1021 agagtatgac gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat
     1081 gaaagaaaat ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca
     1141 aattcaagca tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat
     1201 attattttgg aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg
     1261 aataaccctg aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt
     1321 tttaaagaag tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa
     1381 tcgtttctgg gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct
     1441 caccctaccc cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg
     1501 atcacaaatt attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt
     1561 gttgcagtct tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag
     1621 aacatcaaca accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac
     1681 caatcattca aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt
     1741 tattgactgt tattgatagt gacagttttg aacttattga aagagaacca caagccctta
     1801 agcaacttgt catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg
     1861 cactaaaaaa attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt
     1921 tcacacatag tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc
     1981 ccttagtttt tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac
     2041 accatcaagt acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag
     2101 attttttact ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt
     2161 ttaaaggatt gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg
     2221 aacaggttaa ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg
     2281 gaaaaatgtt ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca
     2341 aataatgata agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc
     2401 atatcgacga cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa
     2461 agaacataaa cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag
     2521 aaaatcagtg taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc
     2581 actaatgatg caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc
     2641 tatccaatgt atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg
     2701 gttccagcgg gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct
     2761 catgatgctg atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct
     2821 aaagaaagtt ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta
     2881 ttgccaggta gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta
     2941 gatcctcata cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat
     3001 cagttagtta aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat
     3061 ttgactaaac tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa
     3121 aaaagttttg tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa
     3181 caagttccta ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa
     3241 ttaaaagagt atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat
     3301 gataaaattg caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct
     3361 cttgaaagtc ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa
     3421 gacaatctaa aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa
     3481 aaagaagcga aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag
     3541 gatcgcgaat tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat
     3601 ggaattttgg atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc
     3661 gagtacaaag attattatac tactggctat aagacagata ttgatactat caacattaaa
     3721 gacaacacca ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt
     3781 ggatacaaaa ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact
     3841 gatgatgcag gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca
     3901 agtaaggcag agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat
     3961 gaattgcata attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa
     4021 gaaatgatgg ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat
     4081 tgtctatgga ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga
     4141 gattaaatgg gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag
     4201 ttcatagagt attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa
     4261 gtagacgttt agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga
     4321 tcatttttcc cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa
     4381 gcgcaacact ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg
     4441 tctgttattc agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc
     4501 tttcgatttt ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg
     4561 ccaagggcat gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg
     4621 aaatgattgg agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga
     4681 atactgctta aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc
     4741 ttctttcctg ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca
     4801 ccagttttgc cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg
     4861 gattttggac ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga
     4921 ttttttgtat gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg
     4981 tgtgagaggg gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa
     5041 ctaattgaaa agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac
     5101 tgaaaatcga caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga
     5161 taacgtccgc atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag
     5221 tttttttctt gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt
     5281 tgccaattat actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga
     5341 gtatagtaag atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa
     5401 acttatacta gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa
     5461 ttcaaggtat agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta
     5521 tcttggttca ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga
     5581 aattgctgtc attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa
     5641 gaaaggtttt aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg
     5701 tacattacgc gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa
     5761 tcaaattgtt attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat
     5821 attagctaaa aaccctttag acattgattt gacccaacga ttagagattg gtgccattat
     5881 tatagtagtg gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga
     5941 aagaaaagct gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga
     6001 tcaattcgag tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac
     6061 gatttacaaa attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac
     6121 aattaaaggt gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga
     6181 gacttcctat tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc
     6241 tgaaacagaa gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc
     6301 acaaaaattt gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg
     6361 gtttctttgg tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta
     6421 taaaagtatt gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat
     6481 gattatacaa aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat
     6541 taataaacta gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga
     6601 gggttgttta actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg
     6661 gaaaaagtct aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt
     6721 caagataggc gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt
     6781 tgtattctct atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt
     6841 tcgaattgat ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt
     6901 gaattatcat cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg
     6961 agttaaagaa gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc
     7021 cggtcaaacg agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag
     7081 cacagattac tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga
     7141 tattattatt aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa
     7201 aagaagcggt gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca
     7261 gtcggttaga ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag
     7321 aataatcgag atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg
     7381 ctttagtcag ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga
     7441 ttcgttctcg gtagttttga aagtttctat agccataggc aattcgtttt agtactttga
     7501 tatggttatt caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga
     7561 tttctgtttg gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct
     7621 tgtagtcttc ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa
     7681 gaaaatcctg gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat
     7741 cgactatctc tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac
     7801 ctttcaattc ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc
     7861 gacgatcccc ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac
     7921 caatgtgttg gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt
     7981 tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga
     8041 cttttatagg gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa
     8101 tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat
     8161 ttttcactga tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa
     8221 tggttttagg ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca
     8281 tacgtgcgat caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga
     8341 ctctgcgagc aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc
     8401 cacagtgttt acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga
     8461 catcattcaa taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt
     8521 gatagtggcg atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac
     8581 atgtttcgtc attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt
     8641 ctagaatatc tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg
     8701 aatggttttc gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa
     8761 aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat
     8821 tttcttcgct tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg
     8881 gtagtagtct tctaaataat cttggattaa caccttcatc tctattttac ttttatattt
     8941 gttggagtcg attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc
     9001 aacaggagtc ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg
     9061 ataaataaaa gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc
     9121 ttaataaatt tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata
     9181 gtaacgtaga atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt
     9241 acatggaatg taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta
     9301 tcgattcaac atttttttac catttctttg attgtttttt ctctaccatg agaagatttg
     9361 cgtcgatgaa atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt
     9421 ttattgattt cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga
     9481 tctttgtgtt cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa
     9541 tgtagaaatc tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt
     9601 atcgacgata ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag
     9661 caattttctt ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt
     9721 tacgatcata tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa
     9781 gaaaagtgta tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga
     9841 tagataagag tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt
     9901 gattggacac atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc
     9961 aattctagag gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg
    10021 cccacttcat acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta
    10081 agctaaaaac gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat
    10141 cattatagaa cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta
    10201 ttgttcgaaa tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt
    10261 ctttaatttc gaatttcgag atagctagat atattgcaag catacattct tgataacaat
    10321 cttgatcata ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga
    10381 gaattgcctc aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag
    10441 aattcataaa tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga
    10501 acgagcaaag aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa
    10561 tttttttgta aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct
    10621 ttccaatttc tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag
    10681 gttctttcaa caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcac
//
LOCUS       DAPCMU010000094        10621 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPCMU010000094 DAPCMU010000000
VERSION     DAPCMU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.097"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            44..667
                     /locus_tag="RD744_001965"
     CDS             44..667
                     /locus_tag="RD744_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557952.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1117..2166
                     /locus_tag="RD744_001966"
     CDS             1117..2166
                     /locus_tag="RD744_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0557953.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2163..3044
                     /locus_tag="RD744_001967"
     CDS             2163..3044
                     /locus_tag="RD744_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0557954.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3047..3817
                     /locus_tag="RD744_001968"
     CDS             3047..3817
                     /locus_tag="RD744_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0557955.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4096..4950
                     /gene="aroE"
                     /locus_tag="RD744_001969"
     CDS             4096..4950
                     /gene="aroE"
                     /locus_tag="RD744_001969"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDU0557956.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5128..6291
                     /locus_tag="RD744_001970"
     CDS             5128..6291
                     /locus_tag="RD744_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDU0557957.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6408..6818
                     /locus_tag="RD744_001971"
     CDS             6408..6818
                     /locus_tag="RD744_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0557958.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6992..9220
                     /gene="topB"
                     /locus_tag="RD744_001972"
     CDS             6992..9220
                     /gene="topB"
                     /locus_tag="RD744_001972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDU0557959.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9391..9675
                     /locus_tag="RD744_001973"
     CDS             9391..9675
                     /locus_tag="RD744_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557960.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9835..10071
                     /locus_tag="RD744_001974"
     CDS             9835..10071
                     /locus_tag="RD744_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDU0557961.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10068..10466
                     /locus_tag="RD744_001975"
     CDS             10068..10466
                     /locus_tag="RD744_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDU0557962.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3716 a         1771 c         2240 g         2894 t
ORIGIN      
        1 aaataatata taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga
       61 agtagttaaa tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa
      121 tccaagtaag tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt
      181 tgcaatgttt ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc
      241 gtccatacca accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa
      301 acaaagccgg gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat
      361 gcttgatgtt tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta
      421 tccgatcgtt tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt
      481 gacatttatt ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg
      541 aataactgat tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat
      601 caaacaatta gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc
      661 taattgagac attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag
      721 aacaagtgat gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa
      781 attcaaaaat tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact
      841 tatcaaaacg tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg
      901 tattaatggt gagactttcc catagataca actttttaga gtgcatttct aaagcttgta
      961 tctatgctag acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt
     1021 aaagacaatg accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat
     1081 aataaataaa aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca
     1141 gttgtagtaa ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc
     1201 gaaaaagaaa gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa
     1261 tacgtgagcc atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa
     1321 gaaggattaa aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag
     1381 agcaagctag ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga
     1441 atcgcaacac ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta
     1501 ggtgctgtaa cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt
     1561 aatgtaacag gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat
     1621 ttgttgccaa atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa
     1681 taccaagtag cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat
     1741 gcggtgcctt ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat
     1801 tttatctatg ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa
     1861 gcaaacaaag caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga
     1921 ctagcaacaa tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca
     1981 gaacttgcga gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg
     2041 gatacagtga tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg
     2101 aaggaaaaag ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac
     2161 aaatgatcgt atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt
     2221 tcatgactta tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag
     2281 gtgctgctgt ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat
     2341 taggcgtagg tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa
     2401 agataccggt tattttagca gggattttag tgatgtcagg attgaactca gttattttgt
     2461 ttgtgatgcg aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc
     2521 atcgctttgg gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca
     2581 tcttgattgc tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga
     2641 caggagacaa cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt
     2701 taggtttaac tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg
     2761 acggatatgc agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca
     2821 tcatcggtga agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg
     2881 tcggcagtat catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga
     2941 cttatctgaa gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga
     3001 agaaagcact gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt
     3061 gacaatcaaa aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt
     3121 ggatgaaatt aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg
     3181 tgctggaaaa agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc
     3241 gatctctttt aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct
     3301 ttctcgagtt ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa
     3361 tttgctactt gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa
     3421 tcaaaaagaa gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt
     3481 ggatactcca gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc
     3541 gacgatcaca gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa
     3601 aacagctaaa gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg
     3661 tctcatgatc acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact
     3721 ccaaaaaggg aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg
     3781 cgaccttttg cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca
     3841 gtagaaggac ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat
     3901 aaacaattaa acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta
     3961 aatagaatat tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac
     4021 ctagacgttt cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa
     4081 caaaggagga ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg
     4141 gcttctcaca gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat
     4201 gcggtgtatt tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt
     4261 cgaacttttg atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct
     4321 tatctggatg aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac
     4381 gaaaagggtc gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa
     4441 gaagctggga tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca
     4501 aaggcagtca ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga
     4561 aacaaccaaa cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct
     4621 tgtaagataa aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa
     4681 agctgtcttc tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg
     4741 ctcttagata taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct
     4801 agagaaacac gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt
     4861 ggcatgttga tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg
     4921 atccatgtga ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc
     4981 tcgcataaac ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt
     5041 ttttattaaa ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt
     5101 atcagtaaga aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat
     5161 aagaaggcga aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa
     5221 cgaacgcgta tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca
     5281 tttgaacatt tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa
     5341 caacaaaaaa ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga
     5401 agaaagcgat cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca
     5461 ggtatctcat tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg
     5521 acttcagaaa cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt
     5581 ggaagtgata cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg
     5641 gatggtcctt cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc
     5701 ccaggggtag gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg
     5761 cgtcaaactt tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc
     5821 aaatcgtttg aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa
     5881 aaagatctga atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat
     5941 gttcttttgc agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga
     6001 cgccaacagc aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc
     6061 cgtgcacctt atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg
     6121 ttcttgacga aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt
     6181 gtgccagtag acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa
     6241 attgataatc aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa
     6301 agatcaagaa gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa
     6361 aaaacgcttt cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc
     6421 tgaacttcaa aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg
     6481 aagcatcagg tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag
     6541 aagaagcaaa aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt
     6601 ttgcagatac gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag
     6661 tcatccagac aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg
     6721 gtaagattga gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg
     6781 atcgatttgg catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct
     6841 ggaaacatca agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc
     6901 gactatacgg tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt
     6961 ccgcaaaaaa ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc
     7021 ttcacaagca aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta
     7081 tgaaatcagc gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt
     7141 ggtagatatg gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa
     7201 tttgcctatt ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt
     7261 ttcgatcgta aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga
     7321 tcgagaaggt gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga
     7381 aaagaactat ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt
     7441 taagaatctc caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca
     7501 gaacgctgat tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg
     7561 aaaagggatt gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat
     7621 ttatcagctg caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg
     7681 tttaattacg acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa
     7741 aacccaagaa gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa
     7801 cggacggatt cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc
     7861 gctatctagt ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact
     7921 tgatgctgct caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt
     7981 ttacattaca gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt
     8041 tttaggaata gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc
     8101 gaaaaaagtt caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg
     8161 ctttgcgaag ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt
     8221 ggctatgttt gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca
     8281 attaaagcta aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg
     8341 catcaagtca aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc
     8401 agaagtagaa ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg
     8461 aacgttgatc acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga
     8521 aattctcaaa gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa
     8581 tcttaagcag aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg
     8641 aatcactctt tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc
     8701 gcgctgggaa gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt
     8761 agagaatatc aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa
     8821 ggtagacttt tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa
     8881 cgatcaatta ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat
     8941 cgctacatgc ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa
     9001 gctgactaaa acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa
     9061 aggttttact ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac
     9121 aatcggtttt tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa
     9181 agaaacatca agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg
     9241 gaatatttat tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa
     9301 tcaagcaaaa acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg
     9361 aaaaaagtgc attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac
     9421 caagcatttc taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa
     9481 acagcatatt caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact
     9541 tatttttttc ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg
     9601 gtggtggtag cggctatgat cattatttat tttctaccag ttattacaca agtatttcat
     9661 caatattttg gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct
     9721 ttgtcatttt ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata
     9781 cttggatata atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa
     9841 aaaatgaaaa agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca
     9901 gaagtaaaag aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct
     9961 gatggttctg tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat
    10021 tcgatcatga atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt
    10081 cgactaaaga aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa
    10141 tgatcggtgt aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag
    10201 tattcaatca agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt
    10261 tagctaaaaa acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt
    10321 tgtttcttca attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta
    10381 tcataatgat taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt
    10441 atttacgaca aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag
    10501 catgtcatta aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat
    10561 aaaaatatgg aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag
    10621 t
//
LOCUS       DAPCMU010000095        10504 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPCMU010000095 DAPCMU010000000
VERSION     DAPCMU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.098"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            12..1130
                     /locus_tag="RD744_001976"
     CDS             12..1130
                     /locus_tag="RD744_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDU0557963.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1258..2634
                     /locus_tag="RD744_001977"
     CDS             1258..2634
                     /locus_tag="RD744_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDU0557964.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3035..4615
                     /locus_tag="RD744_001978"
     CDS             3035..4615
                     /locus_tag="RD744_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDU0557965.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4813..5036
                     /locus_tag="RD744_001979"
                     /pseudo
     CDS             4813..5036
                     /locus_tag="RD744_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5241..5561)
                     /locus_tag="RD744_001980"
     CDS             complement(5241..5561)
                     /locus_tag="RD744_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDU0557966.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5709..7943)
                     /locus_tag="RD744_001981"
     CDS             complement(5709..7943)
                     /locus_tag="RD744_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0557967.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8422..8688
                     /locus_tag="RD744_001982"
     CDS             8422..8688
                     /locus_tag="RD744_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDU0557968.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8688..10415
                     /gene="ptsP"
                     /locus_tag="RD744_001983"
     CDS             8688..10415
                     /gene="ptsP"
                     /locus_tag="RD744_001983"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDU0557969.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3338 a         1835 c         2149 g         3182 t
ORIGIN      
        1 gaggaaatct attgtttgaa aaacttagac agtctaaact aatgttttgg tcgcttgaat
       61 tattgatcat agctgcattg atcatgattt catcagaaat aaactttatt ttcgaaccga
      121 tcgggacatt tttttccacg ttatttgcac ctgtactgat tgcaggcttt ctttactatt
      181 tattgaatcc tattgtcaag ctgttgatga aagcaaaaat caaaagaata tgggctgtcg
      241 caattgtgct gctcctttta gttgcagcaa ttatttggat tttacttagt gtcatcccaa
      301 gtctagtaca gcaaatttcc tcattggcat ctaatatgcc tgatttcatc aaacaggtag
      361 aaagctggtt aaaagaggta gccgaattgc ctttgttcaa ggaagtagat atcaataaat
      421 attttgagca attagacatt tcttacggga caatcattca gcaattcctt agtggagtat
      481 ccaatagttt aggttcgatc gttggaacga tcgcttcggc aaccgtagta atcattacag
      541 caccattcat cttgttctat atgttgaaag acggagaaaa actagtgcca aacatccaac
      601 agtttttccc agaaaaaaga agaaaccaaa ttgtggagtt attggggcaa ctgaataaaa
      661 cattggctaa ttacattagc ggacaagcaa tcgaatgttt attcgttggt accttcacat
      721 ttttaggata tatggtaatt ggtgtggatt atgctttctt atttggggtc attgcgggat
      781 tgacaaacct aattccatat ttaggacctt atctaggact agcacctgca tttctagtaa
      841 cagtcttcaa tgatccggta aaagcattac tttgttgtgt ggttgtgcta atcgtccaac
      901 agttggatgg gaatatcatt tatcctaatg taattgggaa atcactgaaa atccatccat
      961 taacgatcat tatcgttctt ctagtagctg gaaatattgc cggattgctt ggtatcttct
     1021 taggtgttcc tttttacgca atctgcaaga cgatcatttc ctatataatc aaaatcgtca
     1081 aagaagacaa gcaaaatgaa aacaagaaga aaattgctac gacctcgtag gtagttttag
     1141 tacacttgtt tttattgcaa agaaaattgc tttgtgatac cattttaacg tggctatagt
     1201 gccacgtttt tttctatcgt aaactaaaaa ttaattttat aaggagagaa tgcgattatg
     1261 aatgctgacc ctgagagtca gtcgctgtta gcgcagattt tattattgat cgttttgaca
     1321 ttgatcaatg cttttttagc agcttccgaa attgctgttg tttcgatcaa taagaatcga
     1381 atcgagcaaa aagctgatga aggagatgtg aaatctcaaa agcttttaaa aatattgcag
     1441 aatccaaata atttcctatc gacaatccaa gtaggaatca ctttggtcaa cattttgtct
     1501 ggtgcttcac tagctaatac tttatcggaa cggttagctc cggtattagg tggcggcaca
     1561 gcggcaaaaa atattgcaaa tatcattgtg ctggctattt tgacctatgt atcgattgtt
     1621 tttggagaat tgtaccctaa acgtattgct ttaaataaat cagaagaagt cgcaaagttc
     1681 acttcagggc tgatacgctt gattggtgta gtagctaagc ccttcgtctg gctgctttct
     1741 gcttccacaa gtttattggc gcgtatcacg ccgatgactt ttgatgatga agattcaaaa
     1801 atgaccagag atgaaatgcg ttatatgctt gaaaacgaag gcgtattgaa taatgaagaa
     1861 ttagaaatgc tccaaggggt tttttctctt gatacgaaag ttgcccgaga agtcatggtc
     1921 ccacgaacgg atgcatttat gattgatatc aacgacactg tggaagaaaa tgtgaatgaa
     1981 gttttatccg aaaactattc aagaatccct gtttataacg aagacaaaga taaagtagtc
     2041 ggaatccttc atacaaagaa tttactaaaa gctgcccata aattcggctt tgataattta
     2101 gatatcaaga aaatcatgca ggagccttta ttcgttcctg aaacggtctt tattgatgat
     2161 ctgttatatg aaatgaaaaa gacgcagaat cagatggcta ttttattaga tgaatatggc
     2221 ggcgtcgttg ggttagcaac actagaagac cttttagaag aaatcgtcgg agaaatcgat
     2281 gatgaatcag atgaagttga gaatctttat gaaaaaatcg atgaacacga atatatcatc
     2341 caaggacgta tgctgattga tgagttcaat gaagcttttg acagtgatct gcatatgagt
     2401 gatgtagata cgatggctgg ttatttgatt acagcattag gcatgattcc ggatgaaggc
     2461 gaaaagttat cgtttgatgt cgataatatt acgctggttt ctgaagaaat ggaaggttct
     2521 cgaatcctta aaatccgtgt catttttcat gatccggaag aaacagaagc ggaacctgaa
     2581 gaagaacgaa gatacttcag aaaagaattc gaagatgacg agccaagaag ataattggaa
     2641 acttggttat gaaagacgtg ttgcttgaat aaacaagcaa cacgtctttt cttttagtca
     2701 gctgacaaaa aaatatttga ctcttatggt ttggtggctt tttcattcgc tgaatccatg
     2761 ctatactgat tcagttgtag tagttgacaa aggttgtgaa aaagctatcc tgcattaagt
     2821 aataagagca gccaagcaaa aacagctttt cgtatttttg gcttgggtgg gcttattgct
     2881 gataggctgt ctctagaacg ccgtttactc ggaaaaagag actgctacac ggcttttgtc
     2941 acagccctat atgaaattgt ctttctttaa gagatcggac aatctgtgcc tagagaaaat
     3001 ataaataatt gaatggaaga aatggagtta ggttatgaat aatccaaaat taaagcaaca
     3061 agtggacaat cgcagaactt ttgcgatcat ttcccatccg gatgctggga aaacaacgat
     3121 tacagaacaa ttactattgt ttggtggggc tatccgtcaa gccgggaccg taaaaggcaa
     3181 aaaaacaggt aattttgcca aatcagactg gatggaaatc gagaaacaac gtgggatttc
     3241 tgttactagt tctgttatgc agtttgatta tcaagggaaa cgagtaaata tcttggatac
     3301 accaggacat gaagattttt ctgaagatac ttatcgtact ttgatggccg tggatagtgc
     3361 ggtcatggta atcgatagtg caaaagggat cgaagcacag acaaagaaat tattccaagt
     3421 tgtaaaaaaa cgtggcatcc cgatatttac ttttatcaac aaactagatc gtgatggtcg
     3481 tgaaccattg gaattgttag aagaattaga agagctatta gatatcgaat cttatccgat
     3541 gaattggcca atcggtatgg gtaaagggct agaaggtctt tatgatatct accataatcg
     3601 tgtagagttt tatcgtccgg aaaattatca ggatgaacga ttagtagaat tggatgaaga
     3661 tggactgctt cccgaaaatc atcctctaac tgaaaattcc ttatatgaac aagtcttaga
     3721 agaagtagaa ctaatcaaag aggctggaga tgcatttaat caagaaaaaa ttgctcgtgg
     3781 cgatcagaca ccagtatttt ttggttctgc attgactaac ttcggtgtac agactttttt
     3841 agagactttc gttgaattgg caccagctcc ttatggacac aaaacacaag atgatcagat
     3901 ggtaagtcct tatgaggaag aattttcagg tttcgtattc aagatccaag caaatatgaa
     3961 tccagcccat cgtgaccgaa tcgcttttgt ccgaatctgt tctggaacat tcgaacgagg
     4021 aatggatgtc tggttggaac gtacaaataa aaaactgaaa ttaagcaatg tcacacaatt
     4081 tatggcagat tcgagagaaa acgtcgaaaa agcagtagca ggcgatatca tcggtgtata
     4141 tgatacaggc aattatcaga ttggggatac attgttcgaa ggaaaactaa aagttgctta
     4201 tgaagaactc ccttcattta ctccagagct gtttatgaaa gtgactgcaa aaaatgtcat
     4261 gaaacaaaaa tccttccaca aaggaatcta tcaattagtt caagaaggag cgattcagct
     4321 ttataaaaca tatctaacgg aagaatatat cattggagct gttggtcagc tacaattcga
     4381 agtattccaa tatcgtatgt taaatgagta taacgccgaa gtaatcatgt ctccaatggg
     4441 acacaaaatc gctcgctgga tcgatccaga agatttggat gagaagatga gttcaagtcg
     4501 caatatcttg gctcgtgacc gtttcgacca accgttgttc ttattcgaga accaatttgc
     4561 agaacgctgg tttgcggata aatatcccaa tgtaaaatta aaaagtttga tgtaaagaag
     4621 tcttgacaca agctgccctg catcaagtaa taagaatagc caaacaaaaa cagcctctca
     4681 tattttgttt agagccgtga cggattttcc gtgacggctc tttttactat attaaaatca
     4741 tagaaatgtc taatcaaaaa aatataaaaa ttattttgcg tacatatgat taaagaaagg
     4801 agagtgtgac acatggaaac agaatggcaa agacgtgaaa gaaaaaaaca gaaaaagaag
     4861 agatgttacg attatgtcga ttattagatg taaaagactg ggacaaaaac gagcaaattg
     4921 cacaaaaagt acgatcaatc atccataaag aggaaaaagt taaaaataaa gacacgcgta
     4981 gaactgaaaa tgaaaagaga aaagtacaaa agaaaagaga aaaatcaaaa agctagaaag
     5041 aaaaagaggc tgagacagaa gcgtttaact ccaagaaata agctggaatt cacaaaaatt
     5101 tgaaaagcaa ttttcgtgaa tttcagctta tttccgaagg agttgcttct gctttcgccg
     5161 tttatacgtg tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctctttaa
     5221 ttattatgtt caaaatttgt ttacacggct aatgtagcgc ctaattcatg ttttggaatc
     5281 gagatttcaa caaggcctgc caattcgatt actttgattt catcctcgtg gtaaacggcg
     5341 tctttgattt tatctaagaa ataattttta gcatcttcga tttctttcgg ctggatttta
     5401 cgtttgtcat tgatcagcag aatttcatta tgttcagggg cttcagtgta gacaatagtg
     5461 gtatttccgg ctgtgtagac cttgactaga tgggcatcag tgttttcaag ctggtttaat
     5521 acaagacggg aatggctgtt tgttacattt accaatttca tggcgctcac ctcttttatg
     5581 tatttttaat tgcttgtttc tcttattata atttcttttt tataaaatga acagtatttt
     5641 gacttttctg aaaaaagaat gagacggcat ttctcaaaga aataccgtct cacgctataa
     5701 aactggattt attcagccgt ttcttcggct gattctttag ccaaacgttc tggtttagaa
     5761 gtaacgatga tttttccatc tttatccagt tttgctttca actcatggat agaagggtga
     5821 tcaagataaa attcagcgat accatcttcg atttgttctt ggatcgtgcg tcgaagagga
     5881 cgagcaccca tagatggatc atagcctaag tcaactaatt tttctttgac attcgtagga
     5941 acttcgatgt gaagttgctg tgctgccagc atttggttga catcgtctag cattagtgtg
     6001 acgattgtca tcaagttttc ttttgataat gcactgaatt cgatgatccc gtcaaaacgg
     6061 ttcagaaatt ctggtgtaaa gtagttgttt aattggttca atacagaacg agtaacacct
     6121 tcgcgtgccg caccaaaacc aacgtttgct tctactttac cagttccggc attacttgtc
     6181 ataatgatga tcgtatcttt aaagctgacc gttcttcctt gtgcatctgt caatcgacca
     6241 tcatctaaga tttgcaagaa catatgcaaa acatctggat gagctttttc tacttcatct
     6301 aacaagacaa gactgtaagg atttctccgt actttttctg ttaattgacc agcttcttcg
     6361 tatccgacat aacctggagg agaaccaatc aatttagaga cactatgttt ttccatatat
     6421 tcagacatat cgaaacgaat cattgaatct tgtgaaccga atagttcgta ggcaagttgt
     6481 ttcgcaagct ctgttttacc tacaccagtt ggtccaacaa ataagaagga gccgattgga
     6541 cgattctgtt tgtttaatcc aacacggttt cggcgaatcg ctttcgctac tcgatctaca
     6601 gcgttatctt gtccgatgac atgagctttc aagtcatctg ctaagttttt cagttgtgtc
     6661 tgttcttttt cttttaattc acccactgga atacctgtcc gttgttcaac gattttttcc
     6721 atatcttttt cggtaatcac aggtgtttcc tcttcagtga gctgacgttc tttcattttt
     6781 tgaagtttat tgatctgatc gcgataataa gctgcttttt cgaaatcttc ttctcgagaa
     6841 gctaaaactt tttgttcttc tgcttcttgc agttttttct cgattgtttt aggatcaacg
     6901 atttggatcg tcaagttttt cttagaacct gtttcatcta gcaaatcgat ggctttatcc
     6961 ggcaagaagc gatcttggac gtaacgattg gataaagttg cggctgcttc gatggcctca
     7021 tcagtatatt ttacatgatg atagtcttca taacgttttt gcaaaccttt caaaatactg
     7081 atcgtttctt cgacagttgg ttcatctacc cgtacaggtt gcatacgacg ttctaaagca
     7141 gcgtcttttt cgatgatgcg gtactcattt aaggtagtag caccaaccat ttgcaattct
     7201 cctcgagcta aagctggttt taaaatatta ccagcatcca tatttccatc accagcagaa
     7261 ccagcaccga cgatttcgtg aacttcatct atgaatagga tcacattttc tgcttgacgg
     7321 atctcttcaa tcaatttttg catacgttct tcaaattgtc ctcgtatacc agtaccttgg
     7381 actaaagata cgacatctaa acgaatgact tctttgtcca ttaatttttg cggtacatca
     7441 ccatctacga ttttttgtgc taaaccttca acaactgctg ttttaccaac acctggttct
     7501 ccaatcaaga caggattgtt cttggttcgg cgatttaaaa tttcaatgac tcgtttgatt
     7561 tcgtcatcgc gtccgatcac aggatcgata tcaccttgtc gtgcttgttc tgtgatattg
     7621 attccgtatt ctcccaataa accttctgta ttgccttgag gtggctgacc accattgaag
     7681 ttgttgtttc cgccaaattg tgttggtggt gtttgctcat aatttccttg ctgttgcatt
     7741 tggcgagaca tggaacggaa aagatcgtcc aaactgccaa atccaaatgg atcattttgg
     7801 gccatattag tcagacctcc attttgttga tttttgattt tttgataaca gctctgacag
     7861 taatcgagtt gtgtccgctg accattgaca gtggcataca agtgaatcgt tgcctcattc
     7921 ttgccgcagt tttgacaaag catgaatcat tcacgtcctt tcacaaaaat gggtacatcc
     7981 atattttaca tctcgttttt catgaggtaa aagaataacc cctagttttt taagagattt
     8041 atgaggtaga aaaggtgaaa tctctattga ccattcctga cctttgactc ttattatact
     8101 aaaaataaaa agggaagcaa gtaattcgtt tcatatgaaa acaaagaggg tcaaaggaaa
     8161 gaatgataaa cgtcaggaga agagaaagcg acaagaaaaa ggaaataaac acgtttgtta
     8221 taaaatgtga agaaaaggat gaaaaagaat ccggaataat ttgaatactt ttatctgaaa
     8281 ttgaatgaaa actgttgtat ctaacgaaaa aaaatgttaa tctttacaga tgaaagggtg
     8341 gcatttgtta ggattctctg aattactaaa aaagaaaacc ctcacataaa aacaacactc
     8401 acttaaagga gaccgattaa tatggaaaag aaagaatttc acgtagtagc agaaactggg
     8461 atccacgctc gtccagctac actattagta caaacagcaa gcaaatttaa ctctgatgta
     8521 aacttagagt ataaaggtaa atcagtaaac ttgaaatcaa tcatgggcgt tatgtcttta
     8581 ggtgtaggtc aaggttctga tgttactatt actgcagaag gtgctgacga agctgatgca
     8641 atggcagcaa tcgttgaaac aatgaagaaa gaaggcttat ctgaataatg gttgaaatgc
     8701 taaaagggat tgccgctagt gacggtgtag ccgttgcaaa agcttacctg ctagttcaac
     8761 cggatttaac attcagtaag gcaacagtag aagatactgc ggctgaagaa gctcgtttgg
     8821 atgctgcatt agctaaatct actgaagaat tgcagcaaat tcgtgaaaaa gcagcgcaaa
     8881 gcttaggtga agcagaagcg caagtatttg atgcccattt gatggttctt tcagatccag
     8941 aaatggtagg tcaaatcaag caaaatatta aagacaacag cgtaaatgct gaatcagcgc
     9001 taaaagaagt aacggatatg tatatcggta tgttcgaagc aatggaagat aatgcttata
     9061 tgcaagagcg tgctgcagat attcgtgacg ttgctaaacg tattttagcg cacttattag
     9121 gcgttacttt accaaatcca tcaatgatca acgaagaagt ggtcgttgtt gcacatgact
     9181 tgactccaag tgatacagca caattagatc gcaattttgt aaaagcgttt gtaacagaca
     9241 ttggtggacg tacttcacat tctgctatca tggcacgttc tttagaaatt ccagcaatcg
     9301 ttgggacaaa agaaattact gctaaagtca aagaaggcga tattttggct gtcaacggga
     9361 tcgaaggcga tgtaatcatc gatcctacag atgaacaaaa agctgaattt gaaaaagcag
     9421 gtgcagatta cgcagcacaa aaagcagaat gggaaaaatt aaaaaatgca gaaactgtca
     9481 cagctgacgg caagcatttt gaattagctg caaacatcgg aacaccaaaa gatctagttg
     9541 gtgtccataa taatggtgga gaagcagttg gtctgtaccg tactgaattc ctgtatatgg
     9601 attctccaga cttcccaaca gaagatgacc aatatgaagc ttacaaagct gtattagaag
     9661 gtatggaagg aaaacctgta gttgttcgta caatggacat cggtggagat aaagaactac
     9721 catatctgca attacctcat gaaatgaatc cattcttagg atatcgtgca ttgcgtatca
     9781 gtttatctga acaaggcgac gaaatgttcc gtacacaaat gcgtgcattg cttcgtgcat
     9841 ctgttcatgg taatttacgt atcatgttcc ctatggtagc tacactcaaa gaattccgcg
     9901 cagcaaaagc gatctttgaa gaagaaaaac aaaaattgat aagtgaaggc aaggaagtat
     9961 ctgatacgat ccaagtcggc atcatgatcg agattccagc agcagcagtc ttagcagaca
    10021 agtttgcaaa agaagtagat ttcttctctg taggtacgaa cgatttgatt caatacacaa
    10081 tggcagcaga ccgtatgaac gaacgcgttt cttacttgta ccaaccatat aacccatcaa
    10141 ttttacgttt gatcaaaaat gtaattgatg cagcacacgc tgaaggaaaa tgggcaggta
    10201 tgtgtggaga aatggctgga gatcaaacag ctgttccatt gctagtaggt atgggtctag
    10261 atgagttctc aatgagtgcg acatctattc ttaaaacgcg tagtttaatg aaacgcttgg
    10321 acacagcgaa aatggctgaa cttgctgatc gtgcattgaa agaatgcgat atgatggaag
    10381 aagtcgttga acttgttcat gaatatgtaa aataaacaac gtatcaaaag ctggtgtggc
    10441 ttgtaaaaga gctacgccag tttttttgtt cttttttaag aaaacgtttt tgtcagtaag
    10501 aata
//
LOCUS       DAPCMU010000096        10475 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPCMU010000096 DAPCMU010000000
VERSION     DAPCMU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.099"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            11..157
                     /locus_tag="RD744_001984"
     CDS             11..157
                     /locus_tag="RD744_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDU0557970.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(219..2030)
                     /locus_tag="RD744_001985"
     CDS             complement(219..2030)
                     /locus_tag="RD744_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDU0557971.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2027..2392)
                     /locus_tag="RD744_001986"
     CDS             complement(2027..2392)
                     /locus_tag="RD744_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDU0557972.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2555..2950)
                     /locus_tag="RD744_001987"
     CDS             complement(2555..2950)
                     /locus_tag="RD744_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDU0557973.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2968..3930)
                     /locus_tag="RD744_001988"
     CDS             complement(2968..3930)
                     /locus_tag="RD744_001988"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDU0557974.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3930..4142)
                     /locus_tag="RD744_001989"
     CDS             complement(3930..4142)
                     /locus_tag="RD744_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDU0557975.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4163..4861)
                     /locus_tag="RD744_001990"
     CDS             complement(4163..4861)
                     /locus_tag="RD744_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDU0557976.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4881..5423)
                     /locus_tag="RD744_001991"
     CDS             complement(4881..5423)
                     /locus_tag="RD744_001991"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDU0557977.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5555..6559)
                     /locus_tag="RD744_001992"
     CDS             complement(5555..6559)
                     /locus_tag="RD744_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDU0557978.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6556..7545)
                     /locus_tag="RD744_001993"
     CDS             complement(6556..7545)
                     /locus_tag="RD744_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDU0557979.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7542..8348)
                     /locus_tag="RD744_001994"
     CDS             complement(7542..8348)
                     /locus_tag="RD744_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0557980.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8514..9470
                     /locus_tag="RD744_001995"
     CDS             8514..9470
                     /locus_tag="RD744_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0557981.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9547..10065)
                     /locus_tag="RD744_001996"
     CDS             complement(9547..10065)
                     /locus_tag="RD744_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDU0557982.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10153..10302)
                     /gene="rpmG"
                     /locus_tag="RD744_001997"
     CDS             complement(10153..10302)
                     /gene="rpmG"
                     /locus_tag="RD744_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDU0557983.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3251 a         2292 c         1788 g         3144 t
ORIGIN      
        1 tgtatctgga atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc
       61 ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa
      121 ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga
      181 agactttcta aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt
      241 tgtgctattt tatctgcctg tctcagattc catggatctg tataaggtct agctactgct
      301 gcagttagcc aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt
      361 tcccctgaag catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga
      421 taacttcctg tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt
      481 tttatgacag gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa
      541 tagcgactgc tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga
      601 atatcaaatg gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct
      661 ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc
      721 catagtattt ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt
      781 ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct
      841 ttttccgctt cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca
      901 ttctgatagg caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct
      961 ggatgctttc cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc
     1021 tttggaatcg gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct
     1081 tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc
     1141 aggttttctt ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt
     1201 cctcgagggt aataagggag cccccttctt gatccactgt atacaatcgg ttccttgcct
     1261 gatggatgat agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta
     1321 aacaatccga cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta
     1381 ggcggtatct tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca
     1441 tttttctttg cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga
     1501 ccgcttgcta aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa
     1561 ccatcttttc ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga
     1621 aattcttctt gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta
     1681 gattgctgat ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt
     1741 accgtctgtt cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc
     1801 tgtgttacca atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa
     1861 acagaattca ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca
     1921 ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt
     1981 tctgctacac ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct
     2041 ccatttcgat aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat
     2101 aaacaaaagt aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct
     2161 tcttttcttc ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt
     2221 ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca
     2281 gatctaactg cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc
     2341 tatagccatc ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt
     2401 ccttctttct accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa
     2461 tgaacggcac aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga
     2521 ttcggttttt aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc
     2581 cagaccatcc gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta
     2641 atttatttgc tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt
     2701 cttttcgaat agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta
     2761 aaatatgccc agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca
     2821 ttccttctcg aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta
     2881 gatagacttc ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc
     2941 ataaaaccat gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc
     3001 aatgacacca gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg
     3061 cgggaaagta aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc
     3121 cgatactcct ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca
     3181 agctaaacct aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc
     3241 ttgagccatt tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt
     3301 cagttttgaa tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt
     3361 agcgctagat acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg
     3421 atccacagtg atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc
     3481 gccagcaacg atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc
     3541 gttttctcca gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat
     3601 agcgaatttg attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca
     3661 gtttaagtta tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc
     3721 catgccgatt ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat
     3781 tgattcaatg atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct
     3841 ccatttttgc tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc
     3901 taaatcaatc ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca
     3961 attcggtgct ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa
     4021 cggctatcat acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca
     4081 cccacataga taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc
     4141 atctttaagc acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa
     4201 tacatattac aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat
     4261 agcttgtctg ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc
     4321 caccgacatg tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca
     4381 caataaataa aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa
     4441 accctaagat aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca
     4501 gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat
     4561 aaatcaagaa aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca
     4621 gtatcagtga gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta
     4681 aacgccacca ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga
     4741 tctcaagtcg cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag
     4801 cataggtcca taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca
     4861 tgaaaacctc ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc
     4921 tccggttgcc attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga
     4981 aaattattta aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta
     5041 ttgtagataa tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct
     5101 gattgtggct caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca
     5161 taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt
     5221 tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct
     5281 atcacttttg ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct
     5341 ttttcctttt tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt
     5401 aatcttactt tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga
     5461 aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac
     5521 ctagaaatga ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc
     5581 tgataaaaag tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag
     5641 gtacttcccc acttggtaga atcactctag caaccatatc tgcactaagc agaaaaatgg
     5701 aaccgaacaa accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg
     5761 cagcatgagg agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc
     5821 ttgccaaagc aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta
     5881 atccttgcgc gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc
     5941 ctagcggaat taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac
     6001 cccagattga tcccgctaac catgcattca caaatccata attttcctta gataattgga
     6061 tcgtgcatag caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga
     6121 ttcgattcat acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag
     6181 ctaaaacacc acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa
     6241 agaaacctaa ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag
     6301 aatctgccaa ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag
     6361 ctcctgctaa taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca
     6421 aagaagatga ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg
     6481 taccaatcat taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg
     6541 tccaatatat ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc
     6601 aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt
     6661 aaaatccgcc aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg
     6721 atagtccgtt ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac
     6781 aggtccgact aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa
     6841 ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa
     6901 agatattgct tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa
     6961 tactgctaat tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc
     7021 ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat
     7081 agctacgcca gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa
     7141 accaaatacg agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa
     7201 atcagaagct ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt
     7261 gattcctagt aagcctgaat ctgctaaagg atttcttgtc accccttgca tcaatgcacc
     7321 gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt
     7381 tcgtatgatt tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc
     7441 tgctttttcc acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac
     7501 aaaacagacc aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc
     7561 tcttttggca acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca
     7621 atcgttgcat caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt
     7681 gtaccttctg tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa
     7741 gcatgattaa tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca
     7801 gtcaataact gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc
     7861 aagatcaaga tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt
     7921 ccaccagaca gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac
     7981 gcccactcaa ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg
     8041 tatcgtccgt aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt
     8101 gctaataatg caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta
     8161 ttgacaatga tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta
     8221 gattttccac ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca
     8281 gacatgtttt caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat
     8341 ctcgtcattt cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca
     8401 tttcgtcaag cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat
     8461 atcaggaata ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat
     8521 taaaaacaaa agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt
     8581 gctccccctc ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata
     8641 gtactcatac agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac
     8701 ggatcatcgg cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc
     8761 agtggactgt tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc
     8821 caacgatttc ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt
     8881 tgatttcttc ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc
     8941 caacttatgt tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg
     9001 gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca
     9061 tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt
     9121 gggtgaccaa caactcagcc tttatggtca gcaaaaatcg gtcaagtgga agaatcgtat
     9181 acgacgatct ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt
     9241 ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc
     9301 ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag
     9361 cagtgacaga caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac
     9421 ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa
     9481 aaaaagcatc ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca
     9541 tagattttat tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg
     9601 atgggcatat agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc
     9661 tgaaaaatca atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg
     9721 ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata
     9781 gatatcttct gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc
     9841 cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg
     9901 aagtgaaaga tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat
     9961 tgtatgttcg cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt
    10021 gccgatcaga ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta
    10081 tgattttaga tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga
    10141 actttaaaaa cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata
    10201 tttttgtttt tccaaacgat caggattgtt acgtttgttt ttgcttgtta gatagttacg
    10261 ttctttacaa gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt
    10321 tacaagaatc ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat
    10381 tgctagtgtt tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt
    10441 acttctcaaa caaaatcttt ttctttttgt ataaa
//
LOCUS       DAPCMU010000097        10419 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPCMU010000097 DAPCMU010000000
VERSION     DAPCMU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.100"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(121..606)
                     /locus_tag="RD744_001998"
     CDS             complement(121..606)
                     /locus_tag="RD744_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDU0557984.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    696..1041
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1086..1727
                     /locus_tag="RD744_001999"
     CDS             1086..1727
                     /locus_tag="RD744_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDU0557985.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1746..2381
                     /locus_tag="RD744_002000"
     CDS             1746..2381
                     /locus_tag="RD744_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDU0557986.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2399..3214
                     /locus_tag="RD744_002001"
     CDS             2399..3214
                     /locus_tag="RD744_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0557987.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3533..4969
                     /locus_tag="RD744_002002"
     CDS             3533..4969
                     /locus_tag="RD744_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0557988.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5158..7605
                     /locus_tag="RD744_002003"
     CDS             5158..7605
                     /locus_tag="RD744_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDU0557989.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7835..8902
                     /gene="rlmN"
                     /locus_tag="RD744_002004"
     CDS             7835..8902
                     /gene="rlmN"
                     /locus_tag="RD744_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDU0557990.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9095..10198)
                     /locus_tag="RD744_002005"
     CDS             complement(9095..10198)
                     /locus_tag="RD744_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0557991.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3437 a         1789 c         2270 g         2923 t
ORIGIN      
        1 agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat
       61 attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca
      121 ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga
      181 aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg
      241 atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa
      301 tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa
      361 agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc
      421 aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac
      481 ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac
      541 tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg
      601 actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg
      661 aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag
      721 ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa
      781 cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag
      841 ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa
      901 aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat
      961 ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac
     1021 cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa
     1081 agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat
     1141 tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag
     1201 gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc
     1261 ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga
     1321 tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt
     1381 ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc
     1441 aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa
     1501 agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga
     1561 tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg
     1621 cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa
     1681 caatcataat gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa
     1741 gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag
     1801 atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg
     1861 gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt
     1921 atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag
     1981 tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag
     2041 caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag
     2101 cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt tctggcggac
     2161 aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg
     2221 atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat
     2281 cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa
     2341 atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat
     2401 gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc
     2461 gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat
     2521 aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg
     2581 atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca
     2641 gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg
     2701 gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat
     2761 ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat
     2821 gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca
     2881 accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa
     2941 tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata
     3001 cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc
     3061 ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa
     3121 aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa
     3181 atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg
     3241 tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag
     3301 ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt
     3361 aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga
     3421 aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt
     3481 cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca
     3541 caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca
     3601 agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg
     3661 gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg
     3721 ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt
     3781 agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt
     3841 ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata
     3901 tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat
     3961 cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca
     4021 ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc
     4081 acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt
     4141 agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc
     4201 aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc
     4261 tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata
     4321 tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg
     4381 cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac
     4441 ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg
     4501 aaaaaaaggt tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc
     4561 atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact
     4621 gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga
     4681 gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca
     4741 aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg
     4801 cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt
     4861 tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag
     4921 ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt
     4981 aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat
     5041 taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa
     5101 ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg
     5161 gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa
     5221 cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc
     5281 ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt
     5341 gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc
     5401 attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt
     5461 tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa
     5521 ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta
     5581 gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg
     5641 attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg agcattcctg
     5701 ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta
     5761 caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag
     5821 agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca
     5881 gaaaaagaag ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact
     5941 gctgatgaat ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc
     6001 gacagttttc aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca
     6061 aaacagacca atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca
     6121 ttagaatttg agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat
     6181 ttgcttgatt ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat
     6241 atcggcgtat tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc
     6301 agcttaggac catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt
     6361 aaaattgtga acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg
     6421 gaagcaccga ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt
     6481 atggtttcct ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa
     6541 gtacctttag gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct
     6601 cacttactcg tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc
     6661 acaagtatat taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa
     6721 atggttgaat tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac
     6781 cctagaaaag cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa
     6841 aaattcgcgg caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa
     6901 aacttggaaa acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa
     6961 ttagctgatt tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg
     7021 caaatggctc gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat
     7081 gttatcacag gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc
     7141 ggtacagatt ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga
     7201 gatatgctat tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt
     7261 tctgaccacg aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat
     7321 gaagagaaaa tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa
     7381 gatgagctgt ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc
     7441 cttctccaaa gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg
     7501 gaagctcatg gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag
     7561 caggaagaaa catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa
     7621 gggttgaaaa acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg
     7681 gtttttttcc atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt
     7741 aaagttctat tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat
     7801 gtagcgaaac tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt
     7861 gacgaatgaa gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc
     7921 ccaagtttgg gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct
     7981 atcgaaacaa ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat
     8041 ggtcgtccaa gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa
     8101 catgattgag actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca
     8161 agtaggatgt aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga
     8221 tctgactgct ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg
     8281 caatcttggc gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa
     8341 ttacgacaat gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg
     8401 tgctcggcac attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa
     8461 taacggcttg caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac
     8521 atcgatcatg cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga
     8581 gtatttagag aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa
     8641 tgatagacca gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc
     8701 atatgtcaac ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc
     8761 aaaagctgat gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat
     8821 acgtaaagaa catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat
     8881 gaaaaaaaca gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag
     8941 cagttttctt aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag
     9001 ctataaagct ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga
     9061 tgacagcctg cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct
     9121 ggctttcttg tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc
     9181 gggtgatttc ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg
     9241 cttctaaatc ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct
     9301 ttttcataaa cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg
     9361 tcagtacacc ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag
     9421 tcgtaccgta atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag
     9481 aaaccaatcc ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac
     9541 gatcgtcggc actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca
     9601 tttttccgat ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa
     9661 aagtagattc gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag
     9721 gtgcttcggc aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga
     9781 agagtccgtc cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac
     9841 agttcgaacc aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt
     9901 ttacagtatc gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac
     9961 tttcatgctg gatcaactgt tcaatatttc cataagttga attacccccg taccagagac
    10021 tggcgacaac ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa
    10081 gtgcagtctt ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg
    10141 ctttttcata cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat
    10201 tcatttcctt ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg
    10261 cggctgtaga tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg
    10321 aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac
    10381 gacaataatg aatttttgtt cctcttagta tttctaccg
//
LOCUS       DAPCMU010000098        10353 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPCMU010000098 DAPCMU010000000
VERSION     DAPCMU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.101"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            6..305
                     /locus_tag="RD744_002006"
     CDS             6..305
                     /locus_tag="RD744_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0557992.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="RD744_002007"
     CDS             378..1739
                     /locus_tag="RD744_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDU0557993.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="RD744_002008"
     CDS             1761..2021
                     /locus_tag="RD744_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0557994.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="RD744_002009"
     CDS             2035..2787
                     /locus_tag="RD744_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDU0557995.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="RD744_002010"
     CDS             complement(2881..3486)
                     /locus_tag="RD744_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0557996.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="RD744_002011"
     CDS             complement(3539..4513)
                     /locus_tag="RD744_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDU0557997.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="RD744_002012"
     CDS             complement(4891..5610)
                     /locus_tag="RD744_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0557998.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="RD744_002013"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="RD744_002013"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDU0557999.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="RD744_002014"
     CDS             7161..8558
                     /locus_tag="RD744_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0558000.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="RD744_002015"
     CDS             8639..9079
                     /locus_tag="RD744_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0558001.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="RD744_002016"
     CDS             9227..9553
                     /locus_tag="RD744_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0558002.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="RD744_002017"
     CDS             9554..10303
                     /locus_tag="RD744_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558003.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3285 a         1825 c         2146 g         3097 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aag
//
LOCUS       DAPCMU010000099        10270 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPCMU010000099 DAPCMU010000000
VERSION     DAPCMU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.102"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..277)
                     /locus_tag="RD744_002018"
     CDS             complement(<1..277)
                     /locus_tag="RD744_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDU0558004.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
     gene            605..1084
                     /locus_tag="RD744_002019"
     CDS             605..1084
                     /locus_tag="RD744_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558005.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            1117..3345
                     /locus_tag="RD744_002020"
     CDS             1117..3345
                     /locus_tag="RD744_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0558006.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3474..5117
                     /locus_tag="RD744_002021"
     CDS             3474..5117
                     /locus_tag="RD744_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDU0558007.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(5190..5735)
                     /locus_tag="RD744_002022"
     CDS             complement(5190..5735)
                     /locus_tag="RD744_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDU0558008.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5758..6243)
                     /locus_tag="RD744_002023"
     CDS             complement(5758..6243)
                     /locus_tag="RD744_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0558009.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(6233..7045)
                     /locus_tag="RD744_002024"
     CDS             complement(6233..7045)
                     /locus_tag="RD744_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDU0558010.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(7184..8716)
                     /locus_tag="RD744_002025"
     CDS             complement(7184..8716)
                     /locus_tag="RD744_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDU0558011.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8825..9856)
                     /locus_tag="RD744_002026"
     CDS             complement(8825..9856)
                     /locus_tag="RD744_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0558012.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3411 a         1905 c         1969 g         2985 t
ORIGIN      
        1 acttacattt aaatcgttgt ttcttcagct ccagataagt gcgatactca gacacatcat
       61 tcagtaacaa ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa
      121 gacaatggga acactcttct ggcttgtaca gaagagttcc tttgtaaatc aagcaaattt
      181 gccctttgta tttttcttta gttaaacaat tttcctcgaa atagatattt aggtctagaa
      241 tatttagggt atctttgata agctgagtat gagacatctt gaatcctcct atgaattggt
      301 agccaaatta attctaaagg atttcagggt gtcttttcta tattttttta tacaaaaaag
      361 atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca acatcatctt
      421 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
      481 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
      541 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
      601 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
      661 taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac tgggccttgc
      721 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
      781 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
      841 agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
      901 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
      961 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
     1021 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
     1081 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
     1141 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
     1201 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
     1261 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
     1321 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
     1381 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
     1441 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
     1501 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
     1561 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
     1621 cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat ccgagatgcc
     1681 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
     1741 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
     1801 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
     1861 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
     1921 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
     1981 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
     2041 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
     2101 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
     2161 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa agaaagaacc
     2221 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
     2281 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
     2341 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
     2401 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
     2461 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
     2521 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
     2581 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
     2641 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
     2701 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
     2761 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
     2821 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
     2881 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
     2941 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
     3001 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
     3061 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
     3121 ggtcagccaa aagcattgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
     3181 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
     3241 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
     3301 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
     3361 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
     3421 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
     3481 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
     3541 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
     3601 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
     3661 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
     3721 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
     3781 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
     3841 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
     3901 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
     3961 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
     4021 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
     4081 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
     4141 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
     4201 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
     4261 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
     4321 aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc gcttggcaaa
     4381 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
     4441 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
     4501 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
     4561 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
     4621 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
     4681 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
     4741 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
     4801 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
     4861 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
     4921 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
     4981 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
     5041 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
     5101 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
     5161 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
     5221 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
     5281 acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat atcgataccg
     5341 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
     5401 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
     5461 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
     5521 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
     5581 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
     5641 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
     5701 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
     5761 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
     5821 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
     5881 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
     5941 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
     6001 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
     6061 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
     6121 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
     6181 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
     6241 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
     6301 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
     6361 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
     6421 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
     6481 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
     6541 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
     6601 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
     6661 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
     6721 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
     6781 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
     6841 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
     6901 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
     6961 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
     7021 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
     7081 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
     7141 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
     7201 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
     7261 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
     7321 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
     7381 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
     7441 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
     7501 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
     7561 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
     7621 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
     7681 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
     7741 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
     7801 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
     7861 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
     7921 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
     7981 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
     8041 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
     8101 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
     8161 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
     8221 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
     8281 aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt
     8341 ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg
     8401 ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa
     8461 cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt
     8521 tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt
     8581 aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg
     8641 tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg
     8701 ttatggtctc catcattcac actccattat cttttgttaa gtaatataca aattcaagat
     8761 aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt
     8821 tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca
     8881 tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat
     8941 ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata
     9001 gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag
     9061 gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg
     9121 ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat
     9181 tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac
     9241 gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg
     9301 caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt
     9361 caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag
     9421 ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc
     9481 ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt
     9541 aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag
     9601 tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa tggataatca
     9661 gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat
     9721 cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact
     9781 gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc
     9841 tagttttgct gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca
     9901 tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt
     9961 actagctaaa agatatgaag taagaagata catttttagt tattttatta gtgcagtcat
    10021 tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat
    10081 gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag
    10141 aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat
    10201 attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg
    10261 aagatttatt
//
LOCUS       DAPCMU010000100        10227 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPCMU010000100 DAPCMU010000000
VERSION     DAPCMU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.103"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(183..1031)
                     /locus_tag="RD744_002027"
     CDS             complement(183..1031)
                     /locus_tag="RD744_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0558013.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1030..2097
                     /gene="gnd"
                     /locus_tag="RD744_002028"
     CDS             1030..2097
                     /gene="gnd"
                     /locus_tag="RD744_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDU0558014.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2099..3100
                     /locus_tag="RD744_002029"
     CDS             2099..3100
                     /locus_tag="RD744_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDU0558015.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3097..3381
                     /locus_tag="RD744_002030"
     CDS             3097..3381
                     /locus_tag="RD744_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDU0558016.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3414..4211
                     /locus_tag="RD744_002031"
     CDS             3414..4211
                     /locus_tag="RD744_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0558017.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4225..5058
                     /locus_tag="RD744_002032"
     CDS             4225..5058
                     /locus_tag="RD744_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0558018.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5076..5933
                     /locus_tag="RD744_002033"
     CDS             5076..5933
                     /locus_tag="RD744_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0558019.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5955..6464
                     /locus_tag="RD744_002034"
     CDS             5955..6464
                     /locus_tag="RD744_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0558020.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6501..6902
                     /locus_tag="RD744_002035"
     CDS             6501..6902
                     /locus_tag="RD744_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558021.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7014..7367
                     /locus_tag="RD744_002036"
     CDS             7014..7367
                     /locus_tag="RD744_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558022.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7401..7844
                     /locus_tag="RD744_002037"
     CDS             7401..7844
                     /locus_tag="RD744_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0558023.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7861..8946
                     /gene="uxuA"
                     /locus_tag="RD744_002038"
     CDS             7861..8946
                     /gene="uxuA"
                     /locus_tag="RD744_002038"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDU0558024.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8939..9652
                     /locus_tag="RD744_002039"
     CDS             8939..9652
                     /locus_tag="RD744_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0558025.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9859..10008)
                     /locus_tag="RD744_002040"
     CDS             complement(9859..10008)
                     /locus_tag="RD744_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558026.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3405 a         1626 c         2236 g         2960 t
ORIGIN      
        1 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
       61 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      121 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      181 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      241 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      301 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      361 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      421 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      481 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      541 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      601 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      661 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      721 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      781 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      841 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      901 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
      961 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1021 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1081 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1141 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1201 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1261 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1321 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1381 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1441 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1501 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1561 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1621 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1681 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1741 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1801 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1861 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1921 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     1981 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2041 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2101 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2161 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2221 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2281 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2341 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2401 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2461 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2521 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2581 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2641 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2701 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2761 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2821 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2881 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     2941 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3001 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3061 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3121 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3181 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3241 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3301 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3361 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3421 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3481 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3541 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3601 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3661 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3721 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3781 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3841 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3901 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     3961 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4021 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4081 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4141 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4201 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4261 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4321 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4381 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4441 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4501 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4561 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4621 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4681 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4741 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4801 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4861 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4921 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     4981 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5041 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5101 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5161 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5221 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5281 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5341 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5401 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5461 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5521 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5581 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5641 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5701 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5761 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5821 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5881 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     5941 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6001 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6061 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6121 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6181 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6241 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6301 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6361 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6421 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6481 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6541 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6601 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6661 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6721 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6781 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6841 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6901 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     6961 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7021 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7081 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7141 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7201 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7261 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7321 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7381 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7441 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7501 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7561 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7621 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7681 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7741 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7801 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7861 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7921 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     7981 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8041 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8101 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8161 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8221 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8281 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8341 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8401 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8461 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8521 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8581 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8641 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8701 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8761 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8821 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8881 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     8941 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9001 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9061 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9121 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9181 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9241 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9301 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9361 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9421 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9481 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9541 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9601 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9661 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9721 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9781 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9841 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9901 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
     9961 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10021 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10081 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10141 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10201 ttcgaagaca atagaagaca atgaatt
//
LOCUS       DAPCMU010000101        10155 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPCMU010000101 DAPCMU010000000
VERSION     DAPCMU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.104"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            141..461
                     /locus_tag="RD744_002041"
     CDS             141..461
                     /locus_tag="RD744_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558027.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="RD744_002042"
     CDS             913..1113
                     /locus_tag="RD744_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0558028.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="RD744_002043"
     CDS             1260..4049
                     /locus_tag="RD744_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDU0558029.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="RD744_002044"
     CDS             4096..4623
                     /locus_tag="RD744_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDU0558030.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="RD744_002045"
     CDS             4644..5834
                     /locus_tag="RD744_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0558031.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="RD744_002046"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="RD744_002046"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDU0558032.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8348..9022
                     /locus_tag="RD744_002047"
     CDS             8348..9022
                     /locus_tag="RD744_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDU0558033.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9019..9360
                     /locus_tag="RD744_002048"
     CDS             9019..9360
                     /locus_tag="RD744_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558034.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9390..9749
                     /locus_tag="RD744_002049"
     CDS             9390..9749
                     /locus_tag="RD744_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDU0558035.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3393 a         1811 c         2174 g         2777 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaacggg aaagctagct attatacacc aacagcagga atcccagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag actatcatca
     5341 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     5401 atcctgtgat catcaatgga atgatcgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatattc gcttgtggca gaactgccgg
     5581 gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     6721 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     6901 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gccttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     7201 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     7261 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     7321 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     7381 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgaa atcagtgcct
     7441 tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta ccgttttttt
     7501 agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc
     7561 gttcagctcc gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat
     7621 tgtgacttgt gaccgaggag ttggccggag agcaacgttt atttaaaaaa aagaactgcg
     7681 aactctcgca gttctttttt taaaatacgg gataaaggaa gtttccagca gacaagagaa
     7741 gaaagcaaaa gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca
     7801 aacatataca aagaaggtta ttcaaatgga aatctgattt gatgatgctt ctgctgttga
     7861 aataaagcag catctagtac ctgaaaacaa actaatagta acattcgaag atggagtagg
     7921 tccgtatttt cagcatgcaa tgatccatat gtaagtttaa ttcatccttg aaatcatcgg
     7981 accagataaa gttatgacca atctatccca agcaatctag aagagatatt agtaaaagaa
     8041 catttactgg aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcagtggt
     8101 ctgattgacg gaagcttagg tgtgatcgat ttctctggac caaacaacga aggaatcaag
     8161 gggaatccgg cacgttaaga aaaaaagtat aaaaaactag acacttgatg agcgttcatt
     8221 caattgattg aagggactag gctggtgtca ttttttatac atatattttc tctttcgtct
     8281 tgatagggct tatgggataa tataaggaag aaaatcatcg ttttattcaa aaaggagttt
     8341 ttgacaaatg aaaaaacgta tattatttat cggtgacagc attacagacg caggaagaga
     8401 ccgagcagaa gcaactgatc taggtaaagg ttatccactt ttgattgccg gaaaactaat
     8461 ctcccaatat ccaacgatga aatttgaatg catgaaccgt gggataggag gagacaaaat
     8521 tgcagatctg caagtacgct ggcaagaaga ttgtttagct ttacaacctg actatgtatc
     8581 gattttgatc ggaatcaatg atacctggca taatactagc gatcaggcta tattcggaac
     8641 tgagaaatct gcgcagcaat ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaac
     8701 agcggaaatc tgcaacatca tcttgatgga gccgtttgta cttcctataa ccgacgaccg
     8761 tcatggctgg cgtaaagact tagaccctaa aatccaaatt gttcgtaaac ttgctaagga
     8821 atatggcgca ttgctggttc ctttagatgg catattcaat gctttaggga ttgcttatgg
     8881 tttttcgaaa tatacaagag aagatggggt ccacccaacg attggcggac atgagatcat
     8941 tgcacaaagc tggatagatg caattgccgc agtcgatttt tttagtggag ataaaaatga
     9001 gcgaaagaaa agtgagaaat gatggaaata cggtgggcag taaaagaaga tttacctcaa
     9061 atcaatcagc tgattactga tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac
     9121 ctaaatgata agaataaact attgctcgtt gctgtaacaa aagaaaaacg gattatcgga
     9181 tttgcgcaag cggacgtgca acagtcagaa gaacgggtgg atcagcgacc atcaggaaaa
     9241 ctaatccaac acacattagt aacaaaagaa aatacggaag aaatccaaaa aaaatttgca
     9301 gaatacttga tgctggaagc taaaaaaaga agaataaaat caatttttgc ccagttttaa
     9361 tcaaaaacag taatagaagg agaaatacaa tgagttgttt aggaaatgtc atctggttta
     9421 tattcggcgg actaatcggt ggtcttagct ggttagcagc ggggatactt tggtgtatca
     9481 cgatcatagg tatcccaatc ggtatgcaat gttttaagat cgcctccctt tcttttgcgc
     9541 cttttggcaa agaagtaatt tatcaccagt ctggcatgtc attgatcgtt aatatcatct
     9601 ggctgatcgt ttcaggccta ccaattgctc tcgcacatgt cgtcagcggc atccttctat
     9661 gtatcacgat catagggata ccttttgcca agcaatcttt caaacttgct cgattggcgt
     9721 tgatgccatt cggtgcagaa attgtataaa aagagaataa taaaggttag aaaacgcata
     9781 gagtcaggtg ggatacatgt atttcacctg actctatgtg ttttcctgta taagttctcc
     9841 catgtgactt ttacgtcttt tatttatatg ggattactgt ttatggaaag ttgaaataat
     9901 tttcattaaa gaactctttc tatatttttg aaagcgtagt ggagcttcga atatctggga
     9961 aggacatttt gcagggattg aaaaaagata ttgtcctatc gaaaaattaa tgaatttaga
    10021 caaattgcca gcaacaggct tcaaacttag ttgctatcct gtcaagatca agaaggcaag
    10081 tgcgggttgg ataagagcag gggcaatgat cgaagagaaa aagaaagaat aagccaaaac
    10141 atctgtatgg aagaa
//
LOCUS       DAPCMU010000102        10015 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPCMU010000102 DAPCMU010000000
VERSION     DAPCMU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.105"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(479..703)
                     /locus_tag="RD744_002050"
     CDS             complement(479..703)
                     /locus_tag="RD744_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558036.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            894..3671
                     /locus_tag="RD744_002051"
     CDS             894..3671
                     /locus_tag="RD744_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0558037.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3752..4171
                     /locus_tag="RD744_002052"
     CDS             3752..4171
                     /locus_tag="RD744_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDU0558038.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4183..4653
                     /locus_tag="RD744_002053"
     CDS             4183..4653
                     /locus_tag="RD744_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0558039.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4670..5449
                     /locus_tag="RD744_002054"
     CDS             4670..5449
                     /locus_tag="RD744_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0558040.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5436..6257
                     /locus_tag="RD744_002055"
     CDS             5436..6257
                     /locus_tag="RD744_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0558041.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6274..7323
                     /locus_tag="RD744_002056"
     CDS             6274..7323
                     /locus_tag="RD744_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0558042.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7336..8340
                     /locus_tag="RD744_002057"
     CDS             7336..8340
                     /locus_tag="RD744_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0558043.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8973..9992
                     /locus_tag="RD744_002058"
     CDS             8973..9992
                     /locus_tag="RD744_002058"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDU0558044.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1651 c         2093 g         2911 t
ORIGIN      
        1 ttcttgcaat cttttttgct acgtaatttt tgtaaaaaag tgggttgaga gaaatctaat
       61 tgattttcaa agaaggagta tgaaaatgag aaaaatattt attcgtttat tgtgggtact
      121 atcttagagg aataaataat agtggagaaa actatggttt ggcgcagatt ctatcaggtt
      181 tgatggaacg aagacaacct cccgacatca agtcaaaagc tacttgagat tttgatctga
      241 aaataaaaaa agaaaacaag gtaggaacat caatgtttct accttgtttt cgtaatcaat
      301 gggaaaaaac taagagtggt gtacgtaatc aataaaaagg atggtgacaa cagtacctct
      361 ttactctctg aaagcatcat aactcgtttt tcctcattat gccaataaat tgttgttata
      421 ccacatataa ggatttgtaa tagctaaaaa cagacaggaa agttgaaatc tgtgaatctt
      481 attttgtatg gaaggattta tagaacgcaa aaatcaagat aaagatgaat aaaaatcctg
      541 tttcggctat atattgcgta tctacgccaa taaaacgagt ttgttctgtc atagccatat
      601 aaagcaaagg caaaaataca actacagcga gataagatcc gatgatttct cgaaaagttt
      661 tccaggaagt atgtttcctg tttttatatt tatccgattt cattgaatag tcatagcctc
      721 cttattcttt catatgttga aattccagta taaaaactgg aaggaactgt acgtttttta
      781 gaataaaaat tataagggat aagtaggctg ctgtctataa taagatatat ttttcaattc
      841 tttattaaag attttattga caaaatgaat ccgttttcta tgatggagga ggggtgaata
      901 agttgttgaa gaaggaaatt ttatgttact tagagaacca gacagccttt ttcgatttag
      961 aacatatgaa cgaggtcttt acagcacaaa ggttagctga tcattttggg gtaaaaagaa
     1021 acacagtcag tcattatttg aaccagttaa atgatgagca actactagtc aaaatcaatt
     1081 ctcgaccagt gatttatttc cacaaagaag cttttgaaaa acaatttttt aagttacgca
     1141 cgaattttta tttttctatc gatgatctca aagaggaaca gccttttttc gcgcaaccaa
     1201 aagacttatt ctcttttatg atcggatacg acgccagttt gaaagaaagt atcgaacaaa
     1261 tcaaaacagc tctttactat ccagatggcg gattaccact tttgattaca ggcgaaagtg
     1321 gcacaggtaa aagttattta gttaatttgg tctatcagta ttgtctgctt catgatctgc
     1381 tagaagattc tgcaccattc atcactttta attgtgcaca atatgcagat aatcctgaac
     1441 ttttgactag caatttgttt ggacatgtta agggagcgta cactggtgca gaagaaaata
     1501 aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt tttagacgag gtacatcgct
     1561 taacaccaga aggtcaggaa aaactgttca cctatcttga tcaaggtgtg atttatcgta
     1621 tgggagaacc taatttgtca cgccggatca aaacacgtct cttttttgct acgactgaag
     1681 agattactag tcatttctta acaactttca ttcgaagaat acctatccga atcgaactac
     1741 ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact gatttactct ttttttctcg
     1801 aagaacaaag aaaaatgggg agccctttgt cagtcagtgg accagctttg tcacttttaa
     1861 caagtcagaa attatccgga aacataggag agctgaaaaa tatcgtcaaa gtttcagcag
     1921 caaaagcata tgctgaacag cgtgaacagc ctgaaatcca tgtcacgatc catcatttgc
     1981 aaaaagaact acttgcgcag cctattacta aaaatacagc acagcaagga atctatatta
     2041 cgcaagacca gaccttagag caattaatgg agaagcaaca gcccgaacag cagcgaatcg
     2101 ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa taactgtctg ctatattctt
     2161 gcgaaggaaa attaaagcaa gaagtgattc aattatttga ttttttgctg tttgagacca
     2221 gtagacaaga aaaacacgag ctgctggttt attggacaca atctatccga gagacctttc
     2281 gacaaatgga aactgcctat caaatcaaat tcgatggaaa cagtgtctat gccttgagtt
     2341 actatctcta tcagcgaagc actatcaaat ggttaccgga agaacatgat attatccaac
     2401 taatcaaaga attagaacaa caagtttctc agtcttatcc atctagttat cactatgtcc
     2461 aacgaattct ggaattaagt aagatcaaat tagatattga agacacaagt atggatcgga
     2521 ttttactgac gatctatcta aaaaaagcga aatggtcaaa agaaaaacga ctgccaaaag
     2581 cgatgattgc tgctcacggt tatgcaactg ctagcagtat ggcaaatgta gtaaatcgtt
     2641 tattacaaga ggatctattt gaatcatttg atatgccact agatgtcaca ccacagcaga
     2701 ttgcttcaga aatccttgat tattgcgaaa acagcgatgt ttcaaatgga ttgatcatat
     2761 tagtagacat gggatctctg aaagaaatcc atcagttttt taaaaaacag ttatccgtac
     2821 cgcttttgat tttgaataat gtgacgacac ctttagcaat cacagtaggg gaatgcctgc
     2881 aaaaaaatgt atcattagaa gagatagcag aagagactgt tcgacagatc cagccagaat
     2941 ggcgactgct ctatccagag gaaaacaaac ccaaagcact cattactact tgcttcactg
     3001 gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa aagcttacct acggcttgtc
     3061 agttgaaaat catcccatat gaatatcagc aattaaaaga taagaaaaac agtgacccat
     3121 tattttccat atacgaagtc gtaggaatga ttggcaccac agatcctgat atcaccaata
     3181 tcgactatct ttcattagaa gacctcatct cgggggaaaa gatgtctgta ctagctgaat
     3241 ggttggacac tactatgaat gcttcagaga aggaaatatt caatcagcgc atcattcgta
     3301 atttttcatt ggaaaaagta ctggattccg tgactattct agatacagag aaggtgatgg
     3361 gagaaatcga tcattttatg agagaattag aggtgcagtt gaatcgaagc atcagcaatc
     3421 ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat tgaacgattg atccgacaaa
     3481 tgccaatcga aacctatcaa ggattagaga aatggaagca gtgtcaaaaa gaagggttat
     3541 ctgctattaa atctgcgttt agtgtcattg aagagcatta tagtgtcatg atccccaatt
     3601 ctgagattgc ctatatctat gatattttga gtgaagatac agagtttctc tcgatagaag
     3661 aagaattttg aacataagca agttgttgcc actaattttg gcatgcaact tgctttttta
     3721 gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga gaaattatct tagcttccca
     3781 tggaaaattt gcttcaggga tattagattc attagagctg atctatggga aaaatcattc
     3841 tattactgta ttcgattgct atactgatga gaattttgat ttagcggaaa cagtaaggca
     3901 gctttttatg aggtacaaag ataaagaaat cattgttctg acggatcttt ttggcggaag
     3961 tgtcaacaac gaattcttac aatatatcaa tcgggatcat gtctatctcg ttgccggatt
     4021 gaatttgcca ctattgattg agctagtatc tcggttggac gctgaagtag agacaactgt
     4081 tttgatccag caggtgctaa ctgattcaaa aaaaatgatc cagttttgta acgagcggct
     4141 caatcagaaa atagaagaag acgaatttta ggaggaagaa aaatgattgt attgacaaga
     4201 gtcgaccatc ggttattaca cggacaagta gctttttcat ggacacaaac aataggcgca
     4261 gactgtattt tgattgctaa tgatgatgta cctacgaatg agatacgaaa gacaacgatc
     4321 aaattagcca agccacaagg agtcaaacta gtgatcaaat caattgatga ctcgattgcc
     4381 gcgttgaaaa gcggggtgac agataaatat aagttattca tcgtagtgga atcgatcgag
     4441 gatgcatata agctggcaga agcgtatcca gaaatcaaaa ccatcaactt aggagggatc
     4501 aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa atattttacc agaagaggag
     4561 gaacgcttga aacgattaat tgacaaacaa atagaagtag aaatccgtca agtaccagca
     4621 gataaaaaag tggatgtaag agaagttttg taagaaagga agaacctcaa tgattgtaca
     4681 agcagtctta ttaggaattg ttgcatttgt cgcacaatca gaatatgcac tagggacatc
     4741 gttgctttct cgtccgattg tgacggggct gtttacagga atcgtgttag gtgatgtaaa
     4801 aactgggatc attatgggcg caactttaga gttagcattt atcggatcgt tctcagtagg
     4861 tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta gggacggctt ttgctattac
     4921 agctggagcc ggtacagaaa ccgcgctttt actaggtttg ccgattgcta cgttgacatt
     4981 gatattgaaa aatatttact tagggctttt gattccaatt atgaaccata aagcagacaa
     5041 ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg cacttgcttg ccggtatcgg
     5101 tctttccttg atgcttggtg ttgtggtaat ggtttcttat atggtgggaa gcaatacgat
     5161 cggaaatcta ttaaatatga ttcctgactt cgttcaaaag gggttgtcag tagctacagg
     5221 gttgattcca gctcttggtt ttgcgatgct tgctcgtttg ctgatcaaca aacaagttgc
     5281 accatatttt ttcttaggtt ttgctatagc ttcttactta gagattcctg tgaccggagt
     5341 agctattttt ggtgcgattt tagcagttgt ggtagtcaat attatgaatg tccgtcaact
     5401 accacttcaa acaactgcag gggaggtaga agaagatgaa gaattctgag atcaaaaaca
     5461 gcaaaataac gaaaaaagag ctaaatcacg tattttggcg ttcttttcaa atggagtttt
     5521 cttggaatta tgaacgtcag atgaatatgg cttatgttta tgcaatgatt ccgattttga
     5581 aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt gaaacggcat ctagagtttt
     5641 ttaacacgac cccacatatc gttacactga ttttaggaat cacggctgca atggaggaag
     5701 aaaataagga tgatcctgaa tttgatgtga ctgcgatcga tagtatcaaa acatctttga
     5761 tgggacctct tgcagggtta ggagattctt tcttttgggg aactttgcga ttgattgcca
     5821 caggtgtggg tacctcttta gccatgcaag gaaatatttt aggccctatc ttgttcattt
     5881 tgattttcaa tattccgcac attctgtttc gctatctagc aacaggttgg ggatatcgct
     5941 taggaacggg ttttttgaaa aagatccagg caaacggtat gatggaaagt ttgacattag
     6001 gcgcgtctat tattggactg atggtcgttg gagcaatgac tgccacgatg atcaccatca
     6061 atattccttt aaaaatcggt tcaggagaaa atgctgtgac tgttcaaagt atttttgatg
     6121 atatcgttcc aaatatctta tgtcttggag catttggcat agtgttttat ctattgaaga
     6181 aagaagtgaa accattaacg atcttgatcg gactagcttt ctttggaatt tttggttcgt
     6241 ggatagggat tttttaaaaa aggagcagag aaaatgttta cgatgcaaga ttatatctat
     6301 gaagaaaagg aagtactaag tacgatcttg aaaaagaatg atttttcgac aagagaacat
     6361 atgaaaaaaa cagtgaattt actgatctta gcaacgggtt catcttataa cgcttgttta
     6421 gcagcgaagc cagcattgga atcttatggt gatcttactg tcgatattca agaacctttt
     6481 tattttttca attatggaaa actttctcct tcaatcgata ctgtgattgc tgtgtcacaa
     6541 agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga tacaaaaaca agggttacct
     6601 gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt tagaagctgc acaaattatt
     6661 gatttaggtg ccggggtaga atcagtaggc ttcgttacaa aaggctattc tgcgactgtt
     6721 ttacagttac tactcttagg gataggaatt ggaataagta aaaataaaat cagcaaaaaa
     6781 atagaacaag actacatgca acagttgcgc aaaataatca accatttacc agcgattatt
     6841 caaaaaacgg aagaattttt tgatggatat caaagtttat ttcgtttagc acagcgtttc
     6901 gtcaccattg gatatggtcc aaactgggga acagctaaag aagcagagac gaaattgact
     6961 gaaacaatcc gcgttccgtc acaaggattt gagttagaag cttatatgca cggcccatac
     7021 ctggaggcag atgcttcgca tttgctattc tttattgaag gggaaagtgt aaataaggaa
     7081 cgttcgcaaa agttgcaaag atatatgagt cggtacgttg gagagactct caccattacc
     7141 acaaaaaaag caagaaacga gaaaacactt ggattagcaa tagaatgtga tgaatatctg
     7201 tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt ataaaaccgc agtagctaaa
     7261 ggtattgact tgaacaaaaa gatttttgaa gactttgata cagtcttgaa aagtaaatta
     7321 taagaggtgg aaatcatgct gaaattcaat gaagaagaac aaatcaaagc aataaaaggc
     7381 gcactagctt tacgtccgca agtagaagaa atcatcgata aactttatgc ggaaaaattt
     7441 gacgctgtct attatctagg aatcggtgga acgtatgctt cctcgatgca agctgtcact
     7501 tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc atgctgcaga atattacact
     7561 actggaaaca aaagattaac aaaagattct atcgttattt tgtcctctgt taccggtaca
     7621 actcaagaag tagtgaaagc agtagaacaa atcaagaaag taggagcgac actgattgga
     7681 tttatcgata aagcgaacag taagttgtct cagctatgcg attttgtggt cacttatcca
     7741 gctcctggaa cagaacaaat caaatttttt atggcagcag atcgattgat gtatttgaat
     7801 ggagaattcg aagcttattc agaatactat gaacagctcg aacgttattt accaacaggg
     7861 ttagtggaag cagagaaaaa agcagatgca tttggcttat catttgcaga aaaacatcga
     7921 cgtgatagta tgcattattt tattggagca gggaatcaat ggggagctgt ttattcatat
     7981 gccatgtgtt actgggaaga acaaagctgg cttccatcga aatctatcca tgctgccgag
     8041 tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc cagtcacatt atttttaggc
     8101 gaagatgaac agagagtgtt atctgaacgt gtggctaaac ttttgccaaa aatttgcagc
     8161 aattatacat tgatagacac aaaagactat ccagtagaag gtatcagtga aaaatatcgc
     8221 ggacgtgttt tgtctttctt attgatgcat gtagtgacac aacgcatcga tgctcatgtg
     8281 gagcagttga actgccatcc attagagata cgccgctact atcgtcagtt tgattattag
     8341 gatagactgg ataaaaagaa tataaaaaat aaagagagca gcgataactg ctccctttat
     8401 ttttttaaaa tattggcatc aattacgtta taaaacacat tttttgcatt atctacagtt
     8461 caacttataa tctgtttaat gggacatgtg ggtgccaacc attttttgtt atgcaatagt
     8521 actatttagt tgttgcatgg atccgtagta aaagtccaag tacaacgagt tgcgccagtt
     8581 tacatagcgt tttgtatcca gaatgttgac taaaacaaaa gttcctatgt caaccgtcaa
     8641 cagagacaat ctgtccgttg caggacctga cagatttaga aatgttcaat ctgtctctta
     8701 tgtattttaa atcaatactc actaggagaa gtttcctaac ctcctaatag taatgctggt
     8761 agtaacattg tcaaaactgc ttcctcttag gaacaagcaa agcataacga gtgtaaaaat
     8821 aaaatgtaga ctattgtaac atatcattga ttatttcaag taaaaacgat aaaagaagta
     8881 aacaaacact taaaaacaat ggaaattagt cccaacttta agaaatgtat actataatgg
     8941 aaattatcaa ttatttattt ggaggaatat ccatgaaaca aaaaatcgca gttcttggtc
     9001 ctggatcttg gggcactgct ttagcacaaa cattggcaga aaacgggcat gatgtccgta
     9061 tttggggaaa tgtacctgaa cagattgatg aaatcaataa gtatcataca aatcagcatt
     9121 ttttacctga tttgacgatc ccagaatcga ttatcggata taaagaattg gcagaagcag
     9181 tggataacgc agatgctatt ttatttgttg ttccaacaaa agcgattcgt tcagtcgcac
     9241 aggaacttat tggtaaaatg aatactaagc caatcattat acatgcaagt aaaggactag
     9301 aacaagatac gcataaacga atctcagaag ttttggctga agaaattcca gaagaaaaaa
     9361 gacaggcaat tgttgtatta tctggtccta gccatgctga ggaagtggca gtacatgata
     9421 tcacaacaat tacagctgca agtattgatc aaaaagcagc agcctatgta caaaaactgt
     9481 tcatgaatga ttatttcagg atttatacaa ataatgatgt gattggcgta gaaactggtg
     9541 cagcactgaa aaatatcatt gcaattggtg ccggcgctat ccatggatta ggattcggag
     9601 atgatgcaaa agcagcaatc atgactcgag gattggcaga aatcagccgt cttggtgtcg
     9661 cgatgggtgc caatcctctg acatttatcg gtttgagtgg tgtgggggac ttgatcgtta
     9721 cttgtacaag tgttcattct cgcaattggc gtgccggtaa cttgttagga cagggacata
     9781 agctggaaga agttctagaa aacatgggaa tggtcgttga gggtgtggca acgacaaaag
     9841 cagcagtaga gctagctcaa cagctaggtg ttgagatgcc aattactcaa acaatctata
     9901 atgttttgta taatggagaa gatatcaaaa aagcagcaaa agaaatcatg ttacgtgacg
     9961 gaaaaatgga gaatgaattt tctcttcgtt aaatagatga tcaatagtta atcgt
//
LOCUS       DAPCMU010000103         9889 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPCMU010000103 DAPCMU010000000
VERSION     DAPCMU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.106"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(50..562)
                     /locus_tag="RD744_002059"
     CDS             complement(50..562)
                     /locus_tag="RD744_002059"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDU0558045.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(576..2873)
                     /gene="recJ"
                     /locus_tag="RD744_002060"
     CDS             complement(576..2873)
                     /gene="recJ"
                     /locus_tag="RD744_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDU0558046.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3015..3458)
                     /locus_tag="RD744_002061"
     CDS             complement(3015..3458)
                     /locus_tag="RD744_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDU0558047.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3459..4247)
                     /locus_tag="RD744_002062"
     CDS             complement(3459..4247)
                     /locus_tag="RD744_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0558048.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4244..5185)
                     /locus_tag="RD744_002063"
     CDS             complement(4244..5185)
                     /locus_tag="RD744_002063"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDU0558049.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5362..5949)
                     /locus_tag="RD744_002064"
     CDS             complement(5362..5949)
                     /locus_tag="RD744_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDU0558050.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6170..7477)
                     /gene="obgE"
                     /locus_tag="RD744_002065"
     CDS             complement(6170..7477)
                     /gene="obgE"
                     /locus_tag="RD744_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDU0558051.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7771..8781
                     /gene="gap"
                     /locus_tag="RD744_002066"
     CDS             7771..8781
                     /gene="gap"
                     /locus_tag="RD744_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDU0558052.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8866..9801)
                     /locus_tag="RD744_002067"
     CDS             complement(8866..9801)
                     /locus_tag="RD744_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0558053.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2720 a         2266 c         1707 g         3196 t
ORIGIN      
        1 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
       61 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
      121 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
      181 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
      241 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
      301 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
      361 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
      421 gtttttcttt tgcataattt acgatttgtt tagtagcttc ccgataagcg tcaccatctg
      481 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
      541 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
      601 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgtcgctgg
      661 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
      721 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
      781 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
      841 tgagaatgga tcaatttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
      901 taggcctcat caggtgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
      961 acttctttca gatccgctaa atcatctgga caatccataa caactagaga gtttggtttt
     1021 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     1081 ataatctcag gcacgaattt caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc
     1141 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     1201 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     1261 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     1321 aaagcgaccg catcaagctg actcgatgca tcggaaagga caaatttcag atgctgttgg
     1381 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     1441 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     1501 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     1561 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     1621 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     1681 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     1741 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     1801 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     1861 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     1921 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     1981 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     2041 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     2101 tgaagtccta tccgttctga atgatgaatg gcatcgattc ctaatgaaac aaggattcga
     2161 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     2221 tctgcaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     2281 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     2341 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     2401 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     2461 ccttcttcga tcttttcttg gtataccgat tggtttggtc catacccatg ctcaaatcga
     2521 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     2581 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     2641 acagcttctt ggattcggct gattgcttta tccatatcgt gtatcagata cggatcatgt
     2701 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     2761 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     2821 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     2881 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     2941 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     3001 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     3061 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     3121 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     3181 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     3241 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     3301 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     3361 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     3421 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     3481 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     3541 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     3601 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     3661 gccttatcga agaaatttgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     3721 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     3781 gaataaaccg ttgattttgc agttgccatt tttccagcca tagaggcgat attaatgata
     3841 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     3901 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     3961 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     4021 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     4081 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     4141 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     4201 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     4261 cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt
     4321 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     4381 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     4441 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     4501 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     4561 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     4621 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     4681 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     4741 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     4801 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     4861 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     4921 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     4981 tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg
     5041 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     5101 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     5161 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     5221 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     5281 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     5341 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     5401 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     5461 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgagtaa tttctccgcc
     5521 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     5581 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     5641 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     5701 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     5761 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     5821 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     5881 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
     5941 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
     6001 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
     6061 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
     6121 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
     6181 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
     6241 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
     6301 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
     6361 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
     6421 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
     6481 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
     6541 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
     6601 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
     6661 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
     6721 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
     6781 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
     6841 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttaggtacga
     6901 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
     6961 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
     7021 gttcgatttt tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc
     7081 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
     7141 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
     7201 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
     7261 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
     7321 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
     7381 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
     7441 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
     7501 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
     7561 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
     7621 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
     7681 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
     7741 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
     7801 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
     7861 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
     7921 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
     7981 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
     8041 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
     8101 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
     8161 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
     8221 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
     8281 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
     8341 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
     8401 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
     8461 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
     8521 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
     8581 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
     8641 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
     8701 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
     8761 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
     8821 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
     8881 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
     8941 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
     9001 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
     9061 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
     9121 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
     9181 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
     9241 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
     9301 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
     9361 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
     9421 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
     9481 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
     9541 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
     9601 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
     9661 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
     9721 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
     9781 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
     9841 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAPCMU010000104         9370 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPCMU010000104 DAPCMU010000000
VERSION     DAPCMU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.107"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..405)
                     /locus_tag="RD744_002068"
     CDS             complement(<1..405)
                     /locus_tag="RD744_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDU0558054.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQ"
     gene            complement(395..2569)
                     /locus_tag="RD744_002069"
     CDS             complement(395..2569)
                     /locus_tag="RD744_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDU0558055.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2585..4927)
                     /locus_tag="RD744_002070"
     CDS             complement(2585..4927)
                     /locus_tag="RD744_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558056.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4949..7492)
                     /locus_tag="RD744_002071"
     CDS             complement(4949..7492)
                     /locus_tag="RD744_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0558057.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7502..7900)
                     /locus_tag="RD744_002072"
     CDS             complement(7502..7900)
                     /locus_tag="RD744_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0558058.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7981..8265)
                     /locus_tag="RD744_002073"
     CDS             complement(7981..8265)
                     /locus_tag="RD744_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558059.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8289..9287)
                     /locus_tag="RD744_002074"
     CDS             complement(8289..9287)
                     /locus_tag="RD744_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0558060.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2664 a         2006 c         1465 g         3235 t
ORIGIN      
        1 ttgattcaat gtcttttcaa aaaagaatgt ttttctctca atttttttga cgtttctatc
       61 atgaatcacg agaaaacttg ggacactttt aacatggtaa cgttgtaaaa aagtttgaag
      121 ttcttcttgt cttggttctt ttcttgtttc aattcgatat acattcctag agaatttttt
      181 gtcgtatctt tccaagaaag attcaacttc ttggcatttt ttacaatcat ttcttgctag
      241 atacacaaaa aatgattgat tcgttttttg aagttctagt acttcttcta aattactatt
      301 gattagtttt ccatttgatt cttcgttagt ttttatcagt ttaaaacaac caaataagag
      361 gaaactaaca ataagtatcc taacaataac tttcttattt cgcatattgt ttctccaaat
      421 ttggtttccg aatatagacg actcgcggtg gttcctggac gtcttcagct gttcgataca
      481 ccacaaattc gcctgttctc aatgctcgaa tgcgattcgg attgaattta aagcggtcta
      541 cgtctcgttt cgtgcctctt tctcctgtcc aatcaacctt tcccatatca gaataacctg
      601 cttcttgtgt cgtcatttcc gttaattcaa tgtcttcttc tgtccccatt aaatctgccc
      661 aataatcaat ttctgtcggg tcatttgtct tcccaataaa gaacgtattc acattcccaa
      721 tcaactgttt tcgaaggaca ggatcaattc tatcaatgtc cgcaagcgtt tgaggggcat
      781 acgccgcatg caccccagcg cctcgtccct tattgactaa gtctataagg ttatcattta
      841 gataagaagc tggttcgtca aatattacta ggactttcga gcgattggct gtttctcctg
      901 cttgtttata cacttctgac gcaaagaaat taatatctcc cacaagcatt tgagccaatg
      961 tccgaataaa ctcactataa atcaatccgt ttaacgagat ataaacaatc tttccttctt
     1021 taacaacttc ttgaatatct aattctcttt ttcctccaga agtatcaaag agttcaccaa
     1081 gctcactatc caataacata ttgatattgg tctgtaaatt ctcagaagtc gcatacaaat
     1141 aaaacgggtt cttcttatgt tcgtaacgaa caaacaaccg tttaaacaac gtttgctcga
     1201 tttctgtcat gagagaaatg ttttctttta ataatagata atacgtttct aagtcaacgg
     1261 ttttacgatc atgttcaacg attcgctctt cacttaactg ttccacatcg gcaaaatcac
     1321 gttctgtttt gtccatcgct tcattctttt tcgttttttt cttcgtggtt ttcttctttt
     1381 ctacgaccac tttttcttta aataaatctg gatttttcaa ctgaatacga tccgcaaaca
     1441 aatgtaagac gtttcgtggc aataaataat gttgtaacca aactagattt ctttcaattt
     1501 tatggctctt ttggaactcg tctaaaagct gaatcgttac ctgtaataac gctttggctg
     1561 ctgtcgaata atacacgctt tctgtttctg ctagattggt taatttatct cgaatggcaa
     1621 tatcattgcc attttttaca ggattataac gcaaatcgcc accgtccaca aagacttcaa
     1681 tcgggcgatt gtatgcctct gccacttctt ttaacgcttg taaagtctct cctgctcctt
     1741 tcccatcgac taagataacg gggatacgaa acttacacgc atgttctgct agtaattgga
     1801 tcaccgtcgt ttttcctgaa cctgtagtcg ctggaataat cacatgttgg ttcaattcac
     1861 taaagttcat cacataaggg agcccatact catctgttcc taatagaatc actttggcta
     1921 atgaagccag ttgtttattc cgcaaagcaa cgtcttttat gacaattgct acctggtacg
     1981 cttgctgata ctctgctgta tatttttggg actcctgaaa tttattcttc cgacgttcct
     2041 tgtattcttt actatttagc aaagctgttt gccgttcttt ctttgaaaca acttttcttt
     2101 tttcaataaa agacgcatac gcaatcaaac aacttcctgc acctgtccca cctaaaagga
     2161 gggcaaaaat cgtttgtcca ttaaactgca acgcatgaga aaacaagttt aatggaaata
     2221 ataagtaaca cgacaaatac cctctaggaa gaacaaaaat aaaacaaaat agatttaata
     2281 gcgtaaaacc taacgcaaaa ataaaaaaaa gagagcttct tcgttttaag aagctctcat
     2341 actcaaagtc ataaatatgt gcacgtgtct cttttttatc tatcataaag acaatcgcta
     2401 aaggaatact gcctacaata aaaaatggat atagacaaac acctaataaa gtgacgataa
     2461 aataaagttg ttgaaaccga ctttttggcg tttctttttc ttgttttctt ttgcgatcga
     2521 cagaaaacat ttgctcaaaa tgtgtcggtc gttttggtgc attcatcata ctatcgattt
     2581 cctcctatcg ttttcgttgt ttcatttctc ttcgataaat cggttgtttt tctcgttttc
     2641 gtttcatttt tgcttgagtt gctcgaaact cactccgtgt cttcggtgct tgttttggtg
     2701 attttgtttg tttcatggtt gagtatgctt tagatacggt tgaaggcgct ttggtttgtg
     2761 ctgttactcg attgactgct gacatatgtt cccgaacccc ctctggacgt cgaacagatt
     2821 tcggtgtggt agaacctgtt gctcgtggta cattcccttt tcttagtggt ttttgtaccg
     2881 catgtttttg tcctgcaact gtccgggttt tcatcgaact tgtttttgtt acgggagtaa
     2941 gtgcctcctt ccgacgacga cttgtttgtc cctgacgacg ttttcgatcg ttcaattctg
     3001 ttctaggcgt atgcttctgg ccataagact tgtgtttttg ctttggattc gctaatgaca
     3061 ccaccattgc agaaccctga ttgcgatacg gactacgaga cgcacctttt ctacttcttt
     3121 ctcttagact aggattgcct tgcaattctc gttgtaataa atcggctgtt tttcttgcct
     3181 gtttctccat tgctgttcgt tgcatacgtt cttgactttg ctcttctgct tgtttcgctt
     3241 gatcatgcaa gtctcttgct ttttgtgcca tacgttccat acgatttggt gctttatgac
     3301 gaggttgttg tgcggattca cgccgaaaag cacttcgtgt ggcttgtttc gccgtttgtg
     3361 gcatacgaac gtctgagcca cccccacgtt ttcgttgacg ttcttgtctc ttccgtgcgt
     3421 tttctttttc tttttgtttc tgtgcttttc tcgcttcttt gcgttctttc aaccgttgtt
     3481 tggcttccct tctcaatttt gcttctgccc caaatggatt tcccgcaaaa gctaacgctc
     3541 ctcgaacacc cattcttcca gtaaatagac tgcctaaaaa gcctcggaac aaataaattc
     3601 ccaacggcgc aaacaaataa acaatgatca agagaatttt ttcccatgga tcgtcttgtg
     3661 tactacttaa ttggaacccg taactaagaa aagaagaaaa aaggatacat gcaaaacctg
     3721 tcattccttt taaaaacgtc aaaataccaa aaactttggc atagttacca aacacattca
     3781 ttgtgacaag aaacaatcct aaaaaaagta agaacggcaa catcggtact aacaatagtt
     3841 gcatagttcc aataatcaat cgaaacatac ttaagacaaa ataaacaacg gtttgaatca
     3901 gattcacgac aaaataaaac aaggcaaatg ctgtattttt ccaactattg taatattgta
     3961 tgttcttgtt tttcagtgta tctgcttcat actctgtgac tttgtcatga attttctccc
     4021 ctatttttgt acctaaatca ttgtccaata aaagattgat tcgatcatat tcttttccag
     4081 catatttgac tttcttctca cgaattgtgt ctacattggt tgtgccataa tttaacaaca
     4141 gatatggttc ataaacatta ctataaaaga cccgagccgc catcaatttg cctgccgttt
     4201 ccactttttt atccgctgtt acctcttctg tttcttttgt ttcataattt gtttcttctt
     4261 caggtgttaa cactggattg accttcacga acgccgtttc cactgtagaa tctaaagaaa
     4321 aaagaacatc aaaaagattc ttatttgtcc cagaatcttt taatacgctt aacccagtaa
     4381 aaataaggat cgtcataaga aagacacgga gtgcttgttt cattcgagcc tctccagcat
     4441 atttgattgc catcataatt gccgaaaaac tgattccaat agttcctgca atacttaaca
     4501 tattgtccgc aattttttgt gttaagtttt gaatgggttc tcgaatggcg gaaataatat
     4561 ctaattcaaa gagaaattca accattgaag cattgaattc tcctaacatt tttacgccac
     4621 tccaaacaaa atttttcatc actcctgcaa cattgtacga tagctttttt ttaaggttca
     4681 ttgactcata gtcattggtc atcaattcaa acgaattctt tttgtaattt ttatacaatt
     4741 gatccacatc agtcgttact tcttttactt ctactttatc gttggcattc tctccttcag
     4801 aacgatcggt ttcagtgaca cccgcaccat cattcatttg atcggcagaa acagtagccc
     4861 cttgtagaca gaaaaaaaga ccccctaata aaaggagcgt ccaccatttc agagattttt
     4921 ttcgcaaaaa aaccacctct tttttgtttt atcgttgttt ttcattttct aacattgctt
     4981 gaattttttc atcttgattt tcagtagaag aaacagccaa taaccaacta tcaaacaaca
     5041 catcaatggc aatcggttga tttcttccca aatgatcctg gaataaacag gttcctgttt
     5101 gtaattgttc catcatttct aaattggctg aattcacttc catatcaaag aaggacaata
     5161 gtttctctcg tgcttctttt tgtttcggac gaaacgcaaa tttgtaagaa attaactctt
     5221 ttttatcttc ttgatcgtaa tctttaaacg cttgcacaac aaggtaaata tccgtattat
     5281 tcgcacgtcc ttttcgcaag ctatcatcaa tcaaatagct tccttgtgcg gtatcttcat
     5341 acccttttgc ctcgtcataa ataatcgcag cgtcttcttc tttgtttgta gaaaagatat
     5401 acgtaaactt cataatgact tccataattg ccattcctgc aagttgttcg ctatttaatc
     5461 gacccgaatc aatctctttt tgtgtcggaa tgcgtattcc ttgtgtccct aacacgttga
     5521 tttgtgaatc aaaacgtatc ggcgtactgt gctcattccc aatgagaatc tttcctaaac
     5581 caacgctatg ccctaaaatc aatttagcta gttctttgtc actttttcga atttcttcga
     5641 taacttgact taacgaatgc ttacctggca tggtgtaaac tacatcgatg gcgtcttgga
     5701 tcaccgtttt cttgtggctc gctgtttctc gattggtatc tacttctcct aacattccta
     5761 acacattttt agcggtttga attgcctctt cttttggtaa aaacaataga ggatcaagca
     5821 tccctcgaca gctttcatct gaagataacg aaataaaatt aattcgttca tacatagcca
     5881 caaactctgg tatgtgtccg aatttttcaa gtgcctttaa aaagaacttt tgggtttcat
     5941 ttttcggatc aatataaaga attttttgac ctaaaaacat cagccataaa aaaatatatt
     6001 ttacaaggac cgattttcct ttacctggtg gaccaataat caacgtattt ccattggtat
     6061 gttccgcgcc ttcaatactt ttctttgtta aattgggata ataccatact agtttactag
     6121 atagcgctaa cgcttctttc gcagatttaa acttttttgc aataacaatc cgtcctaaag
     6181 gaaatccttt cttatttcct acttctttct ttaagtccaa gccataatct gccacaaaac
     6241 ctgtcgtagc gacctgctcg taactacgaa aacgttgcgt acttccaatt aagcattgat
     6301 taaacaacgt tagctgatcc gctaccgatc gataaaattt gaaatctgta gactcaatcg
     6361 caaattcaat atctttgact cgttcttcca attcttcttt tgaagaagca gatacaacca
     6421 gcgtgacggt agaataacac aatcgttttt ctttgcgctt cagcttatct tttacatcca
     6481 ttaaccgttc tgtaccaaaa tttaatacat cgtctgaatc taaaatgccg tcaacggtct
     6541 catattcttt ttcttgttca taaatccgtc cagccatttt attaattttt cgttcatcag
     6601 attttgaatg ctcaaatcga agtcgaatat gcgtatcaat tggaaaagat aagctatcct
     6661 gaagattttg aataaagcta ctcccataca tggctaaagg agcgtccgtt tgacttaaaa
     6721 acgtcgaata atgcgtttga ttcaactgtt caatcgcaat gtatcctgct tcattcgaaa
     6781 tgacgccctc gcaaatttcc attggcgtaa gctgtctttc aggcaatttt tcttctgatc
     6841 gatggaaaaa ataataataa atttgtccaa tctcttgggc agataagcgc tctatcggct
     6901 tataattgga caattccgta tagagctcct tttctaatgt ttcatacgtt ggtaacacac
     6961 tacttatagg aactcgttgt cccgtaatac gatagataaa ctttttaaaa acatgcttaa
     7021 gcaattgaaa atattcgatc ggatcagaca ctttcatttc tgtagtgaaa cgcacaaaca
     7081 gatacgttcg atactcgttt aattgcactt cgtcttttaa aatctttcct gcctgttcaa
     7141 agtacacttt cccaagatct gcgcattctc cctttaccaa atctcgaatg gtttcttcaa
     7201 tgtgcgtatc taggttaaat tgacgtggaa tattgataaa atgatagtca tattcatcat
     7261 gtccaagtat gccttcgcca tcttgaagat agttcttgaa aaaatcaatg tcatttaaag
     7321 gaaatacttg ggaagaaagt tgatagccta cccacacatc atcatttttg gtcaaaacaa
     7381 aatttcggat aatggcgtct atcggaaatt ctaatcgagg aagcttcgtt tttttctttt
     7441 tgaactgcca aatcccgata ataaagacga ccaaaagaaa caaaaacgcc atttactcga
     7501 ctcactcctt ccatttgtgt agggaatacg tcactttttc atggaaaaac ggcacataca
     7561 tttcatgttc aaagcgttgt ttccgtttct gataatagga cgttcgccca attaaatact
     7621 cgaaaaagtg tttattgtcc catttcattg aaaacaatgt ccaaacgaga acaccaatcc
     7681 caaaaatcat aatcaaccag gactttcgaa acagtgtctg caagaaacta atttgattca
     7741 catacgcaat caaaaatagg atcaaaagaa tgaagagaaa taccccacct atgactaatt
     7801 gttccatgcg aatgccatta gaaatccagg gaattttttg tcctttaatc ccgtaaattt
     7861 tgtgagggtg cttaaactcc tttgtatagt tgtattccat gttcactcta cctcctttta
     7921 caaaaaaaga agcgaccaga agttctaatc ttctgatcgc ttttgcagtt tgtctttttc
     7981 ttatgataat tgtatccagg gcacaatcat ttgcgttacc cccacgttct cttcactatc
     8041 cttaataaag ataatgttct ttagccattc accaatatca acggcattcg ataacaataa
     8101 gacgagcatt atcaaaataa agacgccaat aatggcaaac gctgcccgcg caaagccttg
     8161 ggtcgcataa gcaccaacca ttagaacaat cgcaacgatt gccaatagcc aaatgacttg
     8221 tccagataca tcatcaaata acgatttaag atcaaaaagt ttcatttcca ttctcctcta
     8281 agttcatttt attcatttaa cgtagtaaca aaataactat cgccacttgt ttttacttcc
     8341 attgaaaagt ttgattggaa ctcgtttcct ttctcaaaat agaaacgata cttcccttgc
     8401 aaaaagtagt ttccttcttt attttttact gcatgatcca gttgtacatt gatcaattcg
     8461 ccgccttcta accctttttc gttagaaatc aatgccaatt tttcatcatt tttgacatat
     8521 aactcaaaaa accgtttcac aaatgcttcc agcttgattt cttcgctttc agtgaccgct
     8581 tgcccagaag aataaaatcg attttcctga tagttcacag aattggtatt cttactctca
     8641 gaaagattca aaaattgaac gccatcaagt acttttaaaa cattgttttg ataactgact
     8701 ggaaaagaaa gttgactgct tttccaatgt tctccttctg taaaagtaac ctcataaaat
     8761 aggcgataca ttgcttgtgt caaatcgatt tcttctagtt taataaagcg aacatctttc
     8821 gctactcgat tggtagccgt cgttacaaag cctagatcag acgcttccaa tccttgtgct
     8881 aaataatgat tgacggaagc ctcccattcg tttcgttttt ctggcgaata agtaaataac
     8941 ctttcaataa aatgttgtcc ctcgtacttc aattgttccg tagaagacaa ggtttggttg
     9001 acttcagtta acaatgtttc tggattgact tgttttttca ctgctaaacg cgttaatgta
     9061 tccactcgtc caaagtttag gaggagcacg acacccatta acagaaacaa acaaaaaaaa
     9121 gcgcctgaaa atacactcat ggcacttttt tgcgaaaatt ggatcattcg atctgacggt
     9181 tctgttttct ttttttggaa caaaaaacgt ttcttttgtt tcttttcctt ctgttgtttc
     9241 ggtggcttag tttggaacgc ttgtggatta aattttgcat tactcatgag aaatgccata
     9301 ccgttctaac aataatttcg gtattttgtc cacaatcact acttcgtgga caaccacact
     9361 tgccccctcc
//
LOCUS       DAPCMU010000105         9359 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPCMU010000105 DAPCMU010000000
VERSION     DAPCMU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.108"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(90..758)
                     /locus_tag="RD744_002075"
     CDS             complement(90..758)
                     /locus_tag="RD744_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0558061.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            892..1179
                     /locus_tag="RD744_002076"
     CDS             892..1179
                     /locus_tag="RD744_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558062.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1595..3133
                     /locus_tag="RD744_002077"
     CDS             1595..3133
                     /locus_tag="RD744_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDU0558063.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3130..4095
                     /locus_tag="RD744_002078"
     CDS             3130..4095
                     /locus_tag="RD744_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558064.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4082..5404
                     /locus_tag="RD744_002079"
     CDS             4082..5404
                     /locus_tag="RD744_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0558065.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5467..5571
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5645..6388
                     /locus_tag="RD744_002080"
     CDS             5645..6388
                     /locus_tag="RD744_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0558066.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6403..7395
                     /locus_tag="RD744_002081"
     CDS             6403..7395
                     /locus_tag="RD744_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0558067.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7407..8144
                     /locus_tag="RD744_002082"
     CDS             7407..8144
                     /locus_tag="RD744_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558068.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8134..8856
                     /locus_tag="RD744_002083"
     CDS             8134..8856
                     /locus_tag="RD744_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDU0558069.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8940..>9359)
                     /locus_tag="RD744_002084"
     CDS             complement(8940..>9359)
                     /locus_tag="RD744_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDU0558070.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2712 t
ORIGIN      
        1 tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt
       61 aaattaaata aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt
      121 ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc
      181 agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga
      241 aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa
      301 agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt
      361 ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct
      421 gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat
      481 aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt
      541 ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa
      601 ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc
      661 agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg
      721 attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa
      781 aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa
      841 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
      901 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
      961 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
     1021 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
     1081 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
     1141 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
     1201 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
     1261 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
     1321 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
     1381 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
     1441 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
     1501 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
     1561 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
     1621 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
     1681 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
     1741 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
     1801 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
     1861 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
     1921 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
     1981 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
     2041 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
     2101 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
     2161 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
     2221 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
     2281 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
     2341 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
     2401 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
     2461 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
     2521 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
     2581 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
     2641 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
     2701 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
     2761 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
     2821 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
     2881 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
     2941 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
     3001 attaagcgga aaaataacgg aggatcaaat gattgatatg aattaccaag tcaatgtcga
     3061 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
     3121 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
     3181 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
     3241 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
     3301 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
     3361 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
     3421 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
     3481 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
     3541 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
     3601 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
     3661 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
     3721 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
     3781 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
     3841 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
     3901 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
     3961 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
     4021 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
     4081 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
     4141 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
     4201 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
     4261 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
     4321 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
     4381 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
     4441 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
     4501 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
     4561 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
     4621 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
     4681 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
     4741 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
     4801 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
     4861 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
     4921 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
     4981 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
     5041 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
     5101 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
     5161 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
     5221 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
     5281 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
     5341 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
     5401 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
     5461 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
     5521 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
     5581 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
     5641 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
     5701 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
     5761 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
     5821 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
     5881 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
     5941 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
     6001 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
     6061 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
     6121 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
     6181 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
     6241 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
     6301 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
     6361 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
     6421 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
     6481 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
     6541 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
     6601 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
     6661 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
     6721 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
     6781 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
     6841 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
     6901 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
     6961 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
     7021 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
     7081 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
     7141 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
     7201 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
     7261 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
     7321 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
     7381 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
     7441 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
     7501 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
     7561 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
     7621 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
     7681 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
     7741 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
     7801 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
     7861 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
     7921 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
     7981 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
     8041 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
     8101 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
     8161 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
     8221 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
     8281 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
     8341 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
     8401 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
     8461 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
     8521 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
     8581 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
     8641 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
     8701 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
     8761 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
     8821 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
     8881 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
     8941 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
     9001 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
     9061 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
     9121 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
     9181 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
     9241 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
     9301 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DAPCMU010000106         9006 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPCMU010000106 DAPCMU010000000
VERSION     DAPCMU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.109"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..240)
                     /locus_tag="RD744_002085"
     CDS             complement(<1..240)
                     /locus_tag="RD744_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558071.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHF"
     gene            315..518
                     /locus_tag="RD744_002086"
     CDS             315..518
                     /locus_tag="RD744_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDU0558072.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(659..1144)
                     /gene="ssb"
                     /locus_tag="RD744_002087"
     CDS             complement(659..1144)
                     /gene="ssb"
                     /locus_tag="RD744_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0558073.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1147..2046)
                     /locus_tag="RD744_002088"
     CDS             complement(1147..2046)
                     /locus_tag="RD744_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDU0558074.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2059..2652)
                     /locus_tag="RD744_002089"
     CDS             complement(2059..2652)
                     /locus_tag="RD744_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDU0558075.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2728..2877)
                     /locus_tag="RD744_002090"
     CDS             complement(2728..2877)
                     /locus_tag="RD744_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558076.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(2893..5097)
                     /locus_tag="RD744_002091"
     CDS             complement(2893..5097)
                     /locus_tag="RD744_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDU0558077.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5108..6568)
                     /locus_tag="RD744_002092"
     CDS             complement(5108..6568)
                     /locus_tag="RD744_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDU0558078.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(6629..9001)
                     /locus_tag="RD744_002093"
     CDS             complement(6629..9001)
                     /locus_tag="RD744_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558079.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2201 a         1875 c         1507 g         3423 t
ORIGIN      
        1 aaaatgatcg gggtttctaa tttgcatcga ttcaatgact aattgattcc gtttattgaa
       61 atgacctgtg acggcaatat tgtattttcc atcatccata attaacaatt tatcgataat
      121 ttcaatatct gcaactacac aattaatcga ttcatgaatt gtttgtaaag taaagcgaac
      181 caataatggt tgtgttttta ttactcggat tttatcaagc aaaccaacat atttgttcat
      241 cacgatcacc tcgctaaaat tatagcaaaa acagctaaaa ttgagaattt ttattcgatt
      301 ttagatatgc tataatgatc aagacagtaa agtttgtgac ggtggctatc cgattgaaag
      361 tgaggtggtg cgtatgtgcg tacttccaaa atcttacaga aaggagaagc cttttgcctg
      421 ttgaaacggc gctaggatta atgattagtt tcggttcttt gatcgcaaca atcatttttg
      481 gaatcttagc agttgtaaac aacaatcaaa aaaaataacc gtcagcttta gccggtcgac
      541 gattattttt caaaaataat attaagctgg tcaccgtctt aaacggtgct gttaggggag
      601 ttgttagcgc aactcctctt ttcgtatcca ttataacaac tttattgatc ttttacaatc
      661 agaatggtaa atcatcatca ctaatatcga ttgattgtcc gccaaatgga ttttgtgaag
      721 cgttcaatcc attgttgtta ggcgtagcta catcccgttg ttcaaaggtg gtagggcttg
      781 gactgctgga cgattgatca gcgtgcgcac ggctttctgt cgctgcttta ctttctaata
      841 gttggaaatt atcggccaca acttctgtca catacacacg ttgtccttgt tggttatcgt
      901 aggttcgggt ttgaatccgt ccaacaacgc cgatcaaact gcccttttta gctaatgtcg
      961 ccatcgtttc tgctggtttg cgccaaatca cacagttaat aaaatcagct tctcgattgc
     1021 cgttttgatt cgtgaaattt cggttgacag ctagggtaaa agtcgctact gcagtaccac
     1081 tcgcggtata acgtaaatct ggatctttgg ttaagcgtcc tactaaagtt acattattga
     1141 tcatctttat ctctccattt cttgattcat ttgttgtgat cttttaaact gccgttttct
     1201 ttcttgattt tcaattttga tcacactttt cattaggtga tcaatttcgt tgactgtatc
     1261 tgtaaatgaa atggaattcg caaacgtgtt aactgagcga tatttttccc actgcttttc
     1321 caaatagtca tttcctctga tcttatccat ttctctttca taataaaagg acaacggttt
     1381 tgtatcccca cgttttgtaa atgtatttcg aacacttttg tataactctt tcgtatcgat
     1441 attttctagt ttcggaatgg taaataagtc gtagtaatct tttgctctcg aattatctgt
     1501 gccaaagctg aacgtagcac ttagcttttc tgcaataatt tgttctgttg gataagctgg
     1561 aatctccact tttttatctt caaacattaa ttggtgatag tgtgtatcta cttcaggata
     1621 aatagaatct cctgttgtca cgtccatttt aaacggtaca cgtactttct ctaaattagc
     1681 caccaattta atttgaaatc caggataaaa atcggcttct cttgtttcct tcattccttg
     1741 aatagagaaa acaatgcctt cttttgtggg ttttctagtt aaatcattaa aaatgctctt
     1801 caatttttct tcagttaatt tgctatttct aaatgtcgta tcgatatcaa ttgttgtcct
     1861 tctatcgatt ccatattttg atcctaataa aaagccgcct ttcaaaatga agtcattttt
     1921 gtactttgat tcagaaattt gttcaattag acgatccatc ataaaatgcc gttgcactaa
     1981 ctgtgcattt aatcctgtac tctttgctaa atttcttgtt tttgctgcaa attgtgtcgg
     2041 tgtcatcttt ttcctccatc aatataagct ttgcatgtgg tatttcattt ctttttctac
     2101 atgtagtttt gtcatgtaat ctcttaattt acttggatct ctcaatgggt cttccatgta
     2161 ctcttttaat gcttctaatt tcatttcata ttcaaattca tatctcggat tccaaatatc
     2221 acaaagtgta cgctctttat catacatagt aataggattt ccttgaaagg aagttccctc
     2281 tacttttcct agttcataaa cttctttctt cacgaaatga aattgtaact gctgctcttt
     2341 tgtaattgaa cgatcaacgt tatagccatg gggaaaagtt aagtgaatga atttcggcat
     2401 attcgtagaa agctggtaaa tatctaaagt cgtttcatga gagaagattc ccctggaaaa
     2461 tcgttgctga agccaaaata aatcatctat tccatgacca tcctctacat aataaccacg
     2521 atcaactttg atcaattttc cttgattcac caatctttgt acagtggttg ctgcgattcc
     2581 aaatttcctg gcttccttca tagataaatt tccatcgcga atagccaaca attctttcag
     2641 tttatcttgc atttacttcc cctcccttcg ttacaaatac actacatcaa atatatcata
     2701 gtagtttatt tgtaacaaga ttaacgtcta tcgatctaat tcgatttctt tttgaatgcg
     2761 ctgctggttc tttaattgtt gcagtcttct tttatttgca cgtaatttct cttgttctaa
     2821 acgaattttt gctttttgat tttcgatttc ttgttgtttg tgtgttgtat ttcgcatagg
     2881 ataacccctt cattattttg tagtgcggcg tgttcttttc ttatagttcg tttttttctt
     2941 ttcagaaaat tcaaagcctg ttgtaaagtc ttcttttaaa acgatcgttg cttcaaacga
     3001 acctttctga ccttttagtc ctctgactga ttggcttgtt cggccgtttt gaatgatctc
     3061 tttcagttgt gtggctgtga gtttcttttt ggcaatcgtt gtccataatt taaaatcaca
     3121 ggcttcagac gtacatgccg ccacattttg ataaagcaag acttcttgtt cccgacattt
     3181 tggacaaaca cctaaatgac gcgttgcttc ctgtttttca ctggctgctt tcacctcttg
     3241 taacgttgat ccaaaatctg ttgactggat ttgtcccggt acgacttcca acaagtgaga
     3301 gacaaatttt tggatattag ctaagaaagt agcttgtgtt cctttctgct caccaatctt
     3361 taataagtaa ctttcccatt tggcggtcat ttcggcactt gttagcagcg gctctttttc
     3421 cacggcttgg caaagtaaaa tccctttatt tgtcaccaca agcttatttt tttctacctt
     3481 gatatattcc ttttgtttca aagtttcaat aatatttgct cgtgttgctt ctgtaccgat
     3541 tccttctact tctttcaata tggattgtgc ttcttcgcta tccacggttt ttccagctgt
     3601 tttcatcgct gtgatcaacg taccttcggt ataaggttgt ggtggcttcg tttctttttc
     3661 aaggacttgt agatctaccg tcaccgcttc tccttttgtc actttaggaa gtagcggttc
     3721 ttcttccttt tcttttctgt cgctctcttt gccaaacagc tttttccaac ctagatctac
     3781 tggtactttt ccgactgtct ttagctgaag ctgctgaact tgcgtcaaga tcgttgtttc
     3841 ttcataggta tacttttccg caaacatggc caccgttgtt tttaacactt gttcataaat
     3901 agcctgctgc agtggactta attgttcaaa agccgctgcc gtcatgaccg tctttgtagg
     3961 aatgatcgcg taatgttcct ggactttctt attattcaca tatcgactat tcggtgtatg
     4021 aataggagta ggaatggctt cggcctttaa aaagtgtttg tattcgtcta agtgatccaa
     4081 cagatacgca tactcccctt ctgtaatata aggggtatct gttcgtgggt agcttagata
     4141 tttcccttca tacaaccctt gcatggcttc caaagtatct ttggcactag ctttcatgag
     4201 ctgattcatt ttagattgaa ggctggatag agaaaaaaga cgtggacttg ccgcactttt
     4261 tttctcggtt tcaacttggt ggatcgtacc tggctgtttt cccaattgaa cccccttttc
     4321 ttttaaataa tcttcaaatg cttcttgcgt cgcttgggtt tcattcggat caagcttcgc
     4381 atcaaatgcg ccattttgag cgattacttg ggcttttcct tcaaaatatg gttctttttg
     4441 gaagtttcta atcttttcct gtaattgata aatcatgtaa agcgttgggg tttggactct
     4501 gcctaaggaa aaacttcctg gtatgccttt ttgctgcaag agtaaactat acagcgggct
     4561 accgttcatg ccgatcaacc aatcggcgat ctggcgtgct tgtgcttctt caaattttgg
     4621 gtaggtctct tctgcatctc gtaactgctg gaatccctga taaatggctt ctttttctaa
     4681 ggagttgatc caaagtcttt taaatatctt tccatgatcg gcgcctgctt gttgaatgat
     4741 cgtccaagct atcagttccc cttcacgatc gctatctgtc gcaataataa tggtgtcggc
     4801 cgattgaagt tgctgtttca ccaccttgaa ttgctttttc ttatctttag gaacatgata
     4861 gtgataatgt gtagggaaaa taggcaaatg ctctaatgac cattgtttcc aattttcatc
     4921 gtacatatcg ggcgaatcta actcgactaa atgaccaaaa ccatacgtga tcacagtttc
     4981 tcctgaaaac aatcgatctt ggatctcaaa atacccatct tttcgggtag ctttagaaaa
     5041 gctatcggca taagccttcg cttgtgacgg tttttctgct aaaataacgg ttttcatcat
     5101 gacgatttca ctccctttct tgctgtctac cttgttgaaa caacatggca cgtttttgct
     5161 ttctgatctc ttgttggtag atttcatgct tcaattgatc attccaatcc ttttttactt
     5221 caggtacaaa ttcatgttca tgatttttat agcctttgta gtattgttct gctttataag
     5281 agaaaagaat ctgagcatta atatcccctt tttcttgata aaaacgatca aaatcgtacg
     5341 taccatccac gcgttcacaa gcttttaatt cttttgcgac ttctctacaa atatctttca
     5401 aattaatagc ttctattgaa tcgttaacag aaagctgctg accaaaagac gcatttactt
     5461 tgtgagcgga aaccaattgg ttcgttaatt gcagattgtt ttctgtcttg tgtatggcca
     5521 taatagcttc tggtgtgatc ttttcttctt ttgcgacttc ttcataaatg actttgtatt
     5581 cttttggcac ttgccaatca ttagcgattc ctcgttcaca acgtactttc tgattcgtaa
     5641 atggatcaac tgcgcccatt aattgttctt tttcgtagaa aatatgtcct gcacggtcat
     5701 tatccacaca aatctcgatg gattgaggaa aggcttgttt ctttctcaat tcagatactg
     5761 cctcaccaaa ataatgactg atcacatggt gttttaaccc ttccatcgaa actaaccacg
     5821 tatcgtttaa ctgatcacga tttagtgcag catagctaag taaatcaata ctactctcaa
     5881 aaaattttaa ctgcttaggg tcaccgatct ttaggttaaa gccatgattc gctgtcgaat
     5941 ttttatctat atgcttatat gtgcctctct caccaaaacg ttcataatca atgaacgttc
     6001 cttgataact cgcccctact gtctcatttg tctgtggatc tttccataaa aaggcgaccg
     6061 catgatctaa acgatcgtct cgaactaaga aattttgccg ttcatgtttt ggctttagtt
     6121 cggcaatcaa cccttctttt tctgcataat caatcagcca ctctggtagt tttcgttctt
     6181 ccttcagata attccttcct ttttctgtga gtggttgata atcaacgggg tgatctttgt
     6241 acttttctgt ctcaaaatga agattttcca ctcgttctgt tttgtcttca ttctttagaa
     6301 taaaatcagt ggctgcttta aattgttgct tagggtcttg gatctctttg tctatgaaaa
     6361 aaagtttagc taattcaata atgtcgccac taatattttg actgttccaa taaaaacgtt
     6421 gttttcttcg atcaaaaaca aatgaatcat ggtcttctaa gcgatagtct cgtcctttat
     6481 tgactacagc cattccattg tttcgagcaa aatccactat atcgatctgt ttacatcgat
     6541 caataagact catttttttc tcggtcattt taaaacctcc caaaagaaaa aagactatcc
     6601 gtttttcaga tagtcttttt tacttgtttt atacacgttc tttttgtgac aattgttgtt
     6661 cataatagat ttcatttttt cgttttgcca cttctgacac tgtctcaagt gaaaatcgga
     6721 atgaatttcc tagcgcaaat tcttctgaag gtaacgccgt ttggatctct tttccataac
     6781 tttttccttt taaaattact tgattatgct gtggatcttc aataaacgcc gcatccttca
     6841 aatgtaacgt tcgacccgta tcttcttcca ttactttccc gtctttcttg tcgatccaaa
     6901 gtgaaccaac aggaacaata ttcttttgtt ctgccgtttc tacagtattt ttatgctcct
     6961 gcttcacttc tgctggttgg atctctggtt ctctttctct gtattgattg atttcattag
     7021 aaatggtttg aatgaattca tttgcgacac ggctgacatc attcatgatt ttcccacgtt
     7081 ccgcagcttc cttatcatcc aacgtctgac cattcttcgt ccatgctgca atataaggaa
     7141 tagctttctc cgaagtgtcc attccaaaat gtttgcacac aatataagaa gtcatttctg
     7201 cctgcaattc tttagttgcg gtatctaatt ctgaaaattt gtgcattctt gcatgggcta
     7261 attcatgaat acttgtagct aaatgctgcg taggagtatt tcgagtattc atgacgattt
     7321 cttcttcgcc agtaaattcg ttacgaacat atgccccccg tgcttgtcct aattctcttt
     7381 gatagacttc actttctgtc atatctcgaa tttgtattcc aatattgttg gctaccgctt
     7441 gaatcccagc ttgaagttct gactgtcctt ttttatccaa ttgaaaatca aagacacggt
     7501 tcggaaagat tttcggatag tcttcaggtt ttaactgtgt ttgtgtgata tcaaacactt
     7561 tttcaatcgt gtagtaaggt tttttgatca cttcgatttg accactttcg attcgttttt
     7621 tatcttctgg tgtagcttct gtttctcgaa caaagccacg tcctttctca taaaatcctt
     7681 tgaccgtttt tctgacaaag atcttgttcc cttgttcccc ttttttgata tagattcctt
     7741 cttttttcaa cgcagcacga cctaaacaag ccgttgcacc tggaaattgt tcctcaatca
     7801 ataattgatt tctaaaggat cgttctggaa aacgagacat aaaattcaaa agttctttca
     7861 cgtcttcagg agaatgggta tgctttttca ccgcatccag tgcctgttgc gaaagggatt
     7921 tgacttcttg cttaatctct tctgccgttc gtttttgata gcccttttgt tttgtttttg
     7981 tttctttcga ctgcatttgt tctttcgaaa caggttcatt tttgaccgtt tgtttttgtt
     8041 ccttttcagg tacttgttcg cacaattttg ctaactctaa atagatgggt tgattactgt
     8101 tgacgccatc ccctagatcc attcggatct gatcgactac ttctccgttc ttcacttctt
     8161 caaaatagaa tttataataa cctgcttctg cggcgtattg gttatccaac gttttgagtt
     8221 gatcgatcaa ttctaatgtt acgatctccc caccataact tttcaatcct ttttctggat
     8281 cattttcgtt gaattctact cgatagaaat gatcgttttc tttctccttc aaagaatcag
     8341 ttggcgcagc ctcttcaagt aattcacgaa cagctgcttt ttcctcttcc gacgtggctt
     8401 ggtatttttt tgattcctca aatcccccgt tttcattcag ttctaaagca acttttcgat
     8461 agtctttcaa aaaataaatg gttggatttt ttttccattg cgtcactcgt ccgctgtcaa
     8521 taattgcttg tacttcagga agaagatttt cttttccttt ttctgatctt tctttcagct
     8581 gttctaatgt ttccaatttc cgttggtatt ttcggatcgt agcggctgaa aaagtcgatt
     8641 cacctttttc aaagcgttcg atttcttctt tgattcttgg aatattttcg attgtcatat
     8701 ccaggccgcc gtacttgtta tagcccattt cctttagctc ttcttgtctt tcaagttttt
     8761 ctacctgttt ttcttgttgc ttgatctctt ctctcagact atgcactttg ttttctaaac
     8821 gttcttgttg cctaaaccaa gaatgcccat ttctcttatc gttcattggc tgaccgttgg
     8881 tctttctctg gtgttcaaac atcttctcaa tttgttcgtt taattcattt tgcaatcgta
     8941 ctaattttct tttggatttt cccaagcgac tattgtctag tggttctttt tcttctgcca
     9001 tgtccg
//
LOCUS       DAPCMU010000107         9001 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPCMU010000107 DAPCMU010000000
VERSION     DAPCMU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.110"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            213..1427
                     /locus_tag="RD744_002094"
                     /pseudo
     CDS             213..1427
                     /locus_tag="RD744_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1606..1935)
                     /locus_tag="RD744_002095"
     CDS             complement(1606..1935)
                     /locus_tag="RD744_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDU0558080.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1925..2197)
                     /locus_tag="RD744_002096"
     CDS             complement(1925..2197)
                     /locus_tag="RD744_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDU0558081.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2648..3451)
                     /locus_tag="RD744_002097"
     CDS             complement(2648..3451)
                     /locus_tag="RD744_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDU0558082.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3429..4553)
                     /locus_tag="RD744_002098"
     CDS             complement(3429..4553)
                     /locus_tag="RD744_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558083.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4569..5384)
                     /locus_tag="RD744_002099"
     CDS             complement(4569..5384)
                     /locus_tag="RD744_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0558084.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5374..6246)
                     /locus_tag="RD744_002100"
     CDS             complement(5374..6246)
                     /locus_tag="RD744_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDU0558085.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6331..7620)
                     /locus_tag="RD744_002101"
     CDS             complement(6331..7620)
                     /locus_tag="RD744_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDU0558086.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7848..8492)
                     /locus_tag="RD744_002102"
                     /pseudo
     CDS             complement(7848..8492)
                     /locus_tag="RD744_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2793 a         1906 c         1439 g         2863 t
ORIGIN      
        1 atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact aaattttacc
       61 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
      121 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      181 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      241 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      301 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      361 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      421 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      481 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      541 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
      601 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      661 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
      721 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
      781 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
      841 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
      901 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
      961 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
     1021 attatgagta caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag
     1081 caatgatgat tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt
     1141 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1201 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1261 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1321 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1381 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     1441 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     1501 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     1561 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     1621 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     1681 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     1741 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     1801 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     1861 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     1921 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     1981 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     2041 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2101 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2161 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2221 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2281 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2341 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     2401 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     2461 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     2521 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     2581 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     2641 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     2701 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     2761 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     2821 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     2881 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     2941 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     3001 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3061 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3121 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3181 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3241 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3301 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3361 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     3421 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     3481 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     3541 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     3601 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     3661 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     3721 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     3781 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     3841 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     3901 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     3961 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     4021 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4081 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4141 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4201 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4261 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4321 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4381 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     4441 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     4501 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     4561 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     4621 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     4681 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     4741 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     4801 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     4861 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     4921 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     4981 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     5041 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5101 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5161 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5221 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5281 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5341 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     5401 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     5461 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     5521 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     5581 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     5641 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     5701 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     5761 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     5821 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     5881 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     5941 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     6001 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6061 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6121 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6181 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6241 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6301 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6361 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     6421 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     6481 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     6541 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     6601 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     6661 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     6721 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     6781 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     6841 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     6901 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     6961 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     7021 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7081 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7141 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7201 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7261 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7321 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7381 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     7441 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     7501 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     7561 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     7621 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     7681 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     7741 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     7801 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     7861 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     7921 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     7981 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     8041 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8101 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8161 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8221 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8281 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8341 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     8401 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     8461 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     8521 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     8581 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     8641 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     8701 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     8761 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     8821 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     8881 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     8941 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     9001 g
//
LOCUS       DAPCMU010000108         8935 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPCMU010000108 DAPCMU010000000
VERSION     DAPCMU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.111"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            181..1242
                     /locus_tag="RD744_002103"
     CDS             181..1242
                     /locus_tag="RD744_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDU0558087.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1245..2141
                     /gene="murQ"
                     /locus_tag="RD744_002104"
     CDS             1245..2141
                     /gene="murQ"
                     /locus_tag="RD744_002104"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDU0558088.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2155..4137
                     /locus_tag="RD744_002105"
     CDS             2155..4137
                     /locus_tag="RD744_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0558089.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4202..4648)
                     /gene="nrdI"
                     /locus_tag="RD744_002106"
     CDS             complement(4202..4648)
                     /gene="nrdI"
                     /locus_tag="RD744_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDU0558090.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4859..6283
                     /locus_tag="RD744_002107"
     CDS             4859..6283
                     /locus_tag="RD744_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0558091.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6464..7237)
                     /locus_tag="RD744_002108"
     CDS             complement(6464..7237)
                     /locus_tag="RD744_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDU0558092.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7234..8139)
                     /locus_tag="RD744_002109"
     CDS             complement(7234..8139)
                     /locus_tag="RD744_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0558093.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8293..8613)
                     /locus_tag="RD744_002110"
     CDS             complement(8293..8613)
                     /locus_tag="RD744_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDU0558094.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2811 a         1597 c         1892 g         2635 t
ORIGIN      
        1 ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa
       61 tataaactca acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt
      121 ttctttagat atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat
      181 atgtttggct tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc
      241 cgccaaatgg catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat
      301 gatgtttcgg catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa
      361 ctagaattga tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa
      421 gatctaacgc ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc
      481 aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc
      541 acgcctaaag atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct
      601 tggcataatt attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa
      661 aatcaatggc tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag
      721 ttgagaggcc cactatatca aggattgcca actttggaag aacatcgagg gatccatcct
      781 cttgcaggcg ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca
      841 ggtctgtcag aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg
      901 ctccatacgg aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat
      961 cggcaagacg atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg
     1021 cctatcccag caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa
     1081 aagtatttgc gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa
     1141 aaagtgaatg tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa
     1201 gctggtatga actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt
     1261 tagagaacct aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta
     1321 tttctcaagc cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa
     1381 aagaactgac aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg
     1441 gacgtctgat ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg
     1501 aatgcgtacc gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag
     1561 aagaagcaat gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag
     1621 atctaatcaa gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc
     1681 gtacaccata tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa
     1741 cgatttcttg caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg
     1801 atgcaggtcc agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac
     1861 ttatcttgaa tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact
     1921 tgatggtcga tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca
     1981 tgcaagcgac agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa
     2041 atgttaagct ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa
     2101 aattagctga agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca
     2161 aaaaacgaac tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg
     2221 ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat
     2281 gatcttgtaa atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat
     2341 gaacagcttc aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt
     2401 gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat
     2461 tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa
     2521 aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt
     2581 gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta
     2641 gcaaacttag tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg
     2701 atgaacatct tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct
     2761 gcacaagttt ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg
     2821 acagggatga atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct
     2881 ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa
     2941 aaactgcata aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg
     3001 attgccatcg gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat
     3061 ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt
     3121 ctaggcacat tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc
     3181 catgtgcaaa tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct
     3241 ggtgcaggac aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa
     3301 ttaacagaaa tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg
     3361 atttacggag taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt
     3421 atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca
     3481 ggagccgctt tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta
     3541 ttggcagctt atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt
     3601 ttagaaaaag aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta
     3661 gtacctcaaa cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt
     3721 gtttctttag ataaagcaag tgatccagtt tttgcacaaa agatgatggg tgaaggctac
     3781 tttgtagaac cagaaaacgg acatatctat tctccagtta ctggggtagt cagctctgtc
     3841 ttcccaacga aacacgctat tgggatcaca acgccaaatg gtttagaaat cttgcttcat
     3901 atgggaatca acacagttga cttaggcggt acgccatttg atgtgaaagt aacagaaggg
     3961 cagcaagtga cacctgatac gcttgtagca gaagctgact tagaagcgat caaagcagca
     4021 ggcaaagaaa catccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc
     4081 ttggaaaaaa caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat
     4141 gatagaaaca atagaaacga aagaagaaac tgaaatctat ttttcagctt cttctttttt
     4201 attatttatt gttttctgct aaaatctgat aaattcgtgt cacatcagat ggtgttcccc
     4261 gtaattcata atctgccaag aaaggaaagc caaatgtttg tgcatattgt ttggctgtta
     4321 gacaatactg attattgaag tttttatttc cgctaccgac tacaccaaga caaagtttat
     4381 gattgtcttc atgttccaga tattcacgca tcgtttcggt caagatttct gtatcaccgt
     4441 tatctaaacc atttccgccg tctaaataag taggaacgaa agtaaaaaat ggttctgttt
     4501 ctttcgtaaa atcactattt tcatggattt cttttaaagt aacttcagga ttgacaggat
     4561 cttctgcatg cattttttca gcatattctg ctagatgttt ggcaaaggca cgcgtatttc
     4621 ctgaaatcga tatatataaa atgttcatgg atcatcctcc aattctttcc ttttagtata
     4681 gcatcttttt taagtttttt ggtggtgtaa tttggaaagg gactaggcgc tttgcttgta
     4741 taatgagaaa aaagccggta gaattatatt tctattaaag tttttattaa ttaagtaaaa
     4801 ataaaaaagg tttgttatac tttttgataa aagcggtttt attttggagg atgcttacat
     4861 ggatacagag caacagaaaa aaggaattgg tttaattcca cttgctgcct tagtaattgg
     4921 ttcagcgatt ggaggtggcg tatttggtat tatgacggac atctccggat cggctggcgg
     4981 cccagctatt ttcagttggg tactcgtagg aacagcaatg ttgatgctct ccttgtcgat
     5041 caataatatc aataaaaaac gtccagattt aaaaggcggg atctttagtt acgcagaaga
     5101 aggttttggc cgtttcgctg gttttatcag cggttggggt tactggctca catgctggtt
     5161 aggcaacgta gcttttgcaa cattgttgat gagcgcatta ggatatttct tcccagtatt
     5221 tgaaggtggg caaaatctgg catcgatcgt gatgagtaca gtctttttat ggggatatgc
     5281 atggctagtg aatcggggag tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg
     5341 taaattagtg ccattatttt tatttattgt agtagctatc agcacgtttt cgatccaaca
     5401 attcattgat aacttttcta gcgacattat attgaatgcg aatggacagc cgatcccgtt
     5461 ttccagtcaa gtattgagtt gcatcatggt gatgctttgg gtatttgttg gtattgaagg
     5521 agcctctgtc gtaggttcac gagcaaaaaa acaatcagat gttgggaaag caacgattct
     5581 agggatattc ggcttgatta ttatttacgt cttggtttcc atgcttccat atgggacaat
     5641 gacgatgaat cagctgcaaa cgatcaaggc gcagcctgct atgggctatg tgtttgaaat
     5701 gatggttggt aaatggggag cagtcgtgat caacggcggt ttgatcattt ctttagttgg
     5761 tgtttggctc tcatggacga tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg
     5821 acttttgcct aaagcatggg gaaaactcaa caaaaatggg gcaccaactt atgctatcgt
     5881 attgacgacg ttatgtacga atgtcttttt actttcatta ttattcactg acagtgctta
     5941 taactttgct tacacacttg gtacagcagc tattttcttc acgtggcttt tcttaggatt
     6001 gtaccaaatg aaactttctt atcagcggaa agaatgggtc caatttttag tagggctttt
     6061 agcaagtgcc tttcaagtat gggctatgct agtcgtagcc tttaatgaag taatgcttgt
     6121 tcttgttctt ttcttgccag gcatttattt ttacctcaaa gcacgtaagg agcaaaacga
     6181 gcctgtacaa catatccact tagataagat tttacttata ggtgtgacga tggtcggagt
     6241 gattgcaatg gttttatttg ccactgggat gcttcacgta tagaagtata aaagaagtat
     6301 aaagagaggt tgtgagcctg tcgtttattc aaaaacatac taggaaagga gctgtgacac
     6361 aaatgttgtc acagctcctt tttcagaata aacggtgttt aagagacagc caatcggcaa
     6421 taaggctgaa cgtctctttc acagcctctt ttttgatgag tcatcataca ttttttcgct
     6481 taaatacgag atatcctaat gctaagaaca aaacgatgta agctaaatta ccgaatagca
     6541 tttgattagt agaaagatga gtcaattgct tcatgatctc ttcgtttcca atttggtttt
     6601 gcagattcaa catactgatc ggattccatt tgatccactc ccatttttgg atgagtgcaa
     6661 taaggatacc agatatgatt gacgaagcaa aataaaagac gattcctgct gagatggatg
     6721 cgccggaact attgataaag caagcaatca tcaacactaa acttaaaatc agccataaac
     6781 cgatataact acccaaagta taagtgaaca agctttggat cagtgtttga ccagtagaaa
     6841 ccttctccgt gaaggaaatc gaagggaaaa gaatcagttt catcaacaca gtgacaataa
     6901 tagctaaaag gtataaataa acagaataaa taactaatgt gatccatttg cttactaata
     6961 tttctcctcg agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgcttcca
     7021 tcgaaatgat cgtgcttgca gctgcaatca tgataaagac aatccaagat gtggcactaa
     7081 aaagctgagc agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg
     7141 ctgttcctat gagcaaaaca actaatagaa cagataaaac agcagttgac tttttcttga
     7201 tcaatttaaa taattcttgt tttacaagca atgtcatttt tcttcttccc cctttactga
     7261 atccagaatt tccaatagag aagattccaa atcattcgtc agatgctgga cgtctgtcac
     7321 atggatattt tgtgcagaca atagctggat cacggttcct aataattctt cgttttcttt
     7381 taaaagcttg attttatcag gatcttccaa agtatatcca gcttctgtca agatcgttcg
     7441 agcagctaga tcatttgttg tagagataac gatgaaatct tgcgctgttt ggttcaattc
     7501 agccattgta cattgtttga tgatctttcc ttgattgatc aggatcagct catcgaccat
     7561 tttttcaagc tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa
     7621 gtcggtaatg atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc
     7681 atctaaaata acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt
     7741 cattcccaac gagtaggatt tcgctttttt gtgaatgtac ttttccatct tgagctgatt
     7801 cacgatagtg tcgatagcag atacatcgtt ggtttcagag aataacttca gatgatcata
     7861 gcctgttaag aatggataga tacccgggta ttcgatcaat gctcctactt gttccaaccc
     7921 tttatgacta gagggagaaa tctcattttc accaatacag attgttccat ctgagtaatg
     7981 gatcagtcct aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa
     8041 tccaacgatt ttgccagggg ataagctaaa actgacatcg ctgagggctt gtttgtgacc
     8101 aaagttcttg gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat
     8161 agatagtaca agcatacacc tgatgagtgg agaagtcgct aggtcattgg acttatttct
     8221 tattttttca taaaaaaatt gaatattaaa taaaaaggct tattttttct gatcagtact
     8281 tattccaagt gtctagtggc cgctagtaat cttgatgcca atgatactga tgagtagcat
     8341 ccccacaaag aaccaagtgg aaagattcag cttatcatga aacaagacca caccaattat
     8401 gaccgaacca actgcgccaa tccctgtcca gataggataa gagagactca tcggcaaact
     8461 tttgattgcc tttgacaaaa agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga
     8521 ataattgatt ttagaaaaac catcactcca tttcatcatc gttgaccaga atacttccaa
     8581 aattcctgct atgattaatt ccatccatgc catacttaca ttcctcctta actataaaaa
     8641 aatacacaaa aagaggaaaa tcatcccttc aaagacctaa tggaatgatc atcctcttta
     8701 tcctacccgg tggcagattg tatatctttt tctgtagtat agcacaccta tccagatatc
     8761 ggcaatctca tactttttag taaagtgtac caagcactga cggatatatc gttagcggaa
     8821 aagaagatga atgctttgca atttcataga aagaagttgt tatttattaa tcatttaaaa
     8881 aaaacagtaa ctagacctta tttctatata ttatcgggca ataaattaaa aaaag
//
LOCUS       DAPCMU010000109         8761 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPCMU010000109 DAPCMU010000000
VERSION     DAPCMU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.112"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..289
                     /locus_tag="RD744_002111"
     CDS             <1..289
                     /locus_tag="RD744_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558095.1"
                     /translation="VEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTR
                     SAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(386..820)
                     /locus_tag="RD744_002112"
     CDS             complement(386..820)
                     /locus_tag="RD744_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558096.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1052..1762)
                     /locus_tag="RD744_002113"
     CDS             complement(1052..1762)
                     /locus_tag="RD744_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDU0558097.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1752..2612)
                     /locus_tag="RD744_002114"
     CDS             complement(1752..2612)
                     /locus_tag="RD744_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDU0558098.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2617..3255)
                     /locus_tag="RD744_002115"
     CDS             complement(2617..3255)
                     /locus_tag="RD744_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDU0558099.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3270..3569)
                     /locus_tag="RD744_002116"
     CDS             complement(3270..3569)
                     /locus_tag="RD744_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0558100.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3612..5096)
                     /locus_tag="RD744_002117"
     CDS             complement(3612..5096)
                     /locus_tag="RD744_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDU0558101.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5116..5577)
                     /locus_tag="RD744_002118"
     CDS             complement(5116..5577)
                     /locus_tag="RD744_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0558102.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="RD744_002119"
     CDS             complement(5595..6662)
                     /gene="ulaG"
                     /locus_tag="RD744_002119"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDU0558103.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6880..7650
                     /locus_tag="RD744_002120"
     CDS             6880..7650
                     /locus_tag="RD744_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0558104.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7923..8750)
                     /locus_tag="RD744_002121"
     CDS             complement(7923..8750)
                     /locus_tag="RD744_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558105.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2647 a         1848 c         1381 g         2885 t
ORIGIN      
        1 tgttgaattt gacagtgaac tacttaagca atttccacaa gaagaacatt tcatgtataa
       61 agaagcaatt tcccacaaag tgacagagta tttggagcat aatttttttc gtataaagcc
      121 agtgcgtact cgttcggcaa aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa
      181 ttatcgcata gcatttgctt taaaaaaaaa tcatgtgttc attttttata ttagttcgaa
      241 tttgcaaaaa attgtatttg ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg
      301 aggatagatt gagtgatact aagtctatcc tcttgttttt ctaaagaact aagctagaat
      361 tttttctttt gtacgttatt atatgttatt tgtcattacc aaaaagcacc ttttttcttc
      421 tagtatctat gacataagaa ataccaccca tttcctttac ttgaacaaat cctactgatt
      481 cataaaattt ttttgttttc tcagtatcat ctgtcaataa ttcgatttgg actggtccat
      541 aactagtgat cttctccaaa gcttcattca aaagcacttt gcctattcct tggcgctgat
      601 acctcggatg aatgaggata tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac
      661 gaacgattcc gataatcttc ccttccccat tacgctttat cagagattca gattgttcaa
      721 atgctctagc taaccttgca gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata
      781 attcagaaat ctcatcgatc ataagggaat caatattcat ttttttcttc ctttcttata
      841 caattctccc tctaataata cagttctttg actatttaat ctatcacata taactctcct
      901 gtaaagattc tctcgattcc ttaaaattcc tctttccttt tagaatgcat actttttgat
      961 aaataaaaag gttgtgataa attttcttca caacctctgt ttctctctat caagtgttat
     1021 cttttaattt tctaccctta ccctagcatc tctattgccc gtagtatgca tactttccat
     1081 gtttgcgaaa ataatgttta tctagtaaat attgcgggat ttcacatata ttggggttga
     1141 tcagctcgtt ttctttggcc atcagacaaa tttcatctaa gatcaaactg ttttctactg
     1201 ctttcatagg tgaatccccc catgtgaacg gtccatgtcc atagacaagt actccaggga
     1261 cttcgtttgg atctaatttt ctttccttaa acgtctcgac aatcacattc cctgtatgaa
     1321 cttcgtaagc ttctcttact tcttctttcg ttaattggcg tgtacaagga accttaccat
     1381 aaaaagcatc agcatgagtc gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca
     1441 tgacggagtg cgtggaatga gtatgagtaa tagcagttat atcttcaaaa gtttgataca
     1501 aaacaacatg tgtggggaga tctgaagatg gttttaagct atcttcttct attatatttc
     1561 ccgacaaatc tgttaccacc atttgatctg cttgcatgca ttcatatctg acaccgcttg
     1621 gcttaatgac gatgactcct agctcacggt ccacttcact cacattgccc caagtcaatt
     1681 tgactaatcc gtatcgcggc aatgccagat ttgcttcaag gacacgttgc ttcattgctg
     1741 caattttttc ttcacaagac atcatagcca gcctccttca atttagaact taagtagtct
     1801 tttgcctgct ggatttctct tcgaaagtct tggctttttt cactccacat ttcaatcaga
     1861 aatgttccat cataatctag acgtttcaaa tgtcttaata atccaagaaa atcaacacaa
     1921 ccgctaccaa aaggaacatc acgaaactgg cctgaatatt cttttgttac agggtaagta
     1981 tcttttaagt ggatagcagt gatataatcg atccctttct caagttctcg agccggatta
     2041 ttttccggcc atgcggacaa attgcctagg tctggatata cttgtagaaa aggtgaatga
     2101 atctgttctt tgatttctaa aaattttgaa atggagttca taaatggatc atccatgatt
     2161 tccacggaaa gaatgatgcc atatttagaa gcttccttta cgctttcttt tattccttcg
     2221 ataaagtctt ctctggaatt cattgatttc tcttcataat aaacatcgta tccagctatt
     2281 tggattactt tgacgcttag attatgcgcc aaatggattg cttttttcat tagttgtttc
     2341 gctgcttttt tcttttctgg atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa
     2401 caaatcgaat ggatacgtac accggagctg aacatctctt ctctcagctc agctatcttt
     2461 tcttcagacc aatctaatcg tgctaaccgc tcatccgtct catcaatcga catctcgata
     2521 aaatcgaagt ccatttcttt tacaagggaa aaacgttctt tccatgaaat atctttgggt
     2581 aacgcttttt cataaatccc aatttgagcc atttccttac ccccagatac gaatgatctc
     2641 atctttgaag ttttgtgcgg ccttcatcgg atcatttgct gctgtaatac cacgaccagt
     2701 gataaaggta tacacatcga tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc
     2761 agttacagaa acattaaagc ctagttcaat cagttttttg atcttcgata aatctttttc
     2821 accccaagtt tcaccagata acagcgcatc acggctttga tggtagatta cctgccggat
     2881 accgatgtta tgccaatcca tggcttgttc aaaagtccaa ttgccgtaga gttctacttg
     2941 caattcacct atttcttttt gtgctgcctc catagttgga atcgttgcac aacaaatgac
     3001 tgtcatccag tctgctcctg cttttgcaac attactcgcc actgttcctc cagcgtctgc
     3061 acatttcgta tcagcgacta gttttttatc tgggaacata ctgcgaatac agcgaatcgc
     3121 tttttgtcct tcctgtaagc aaagaattgt gcccacttca ataatatcta ctacatcacc
     3181 aactttcata acatctccta atgcatgttc taagtcattg tgatctaacg caatttgcaa
     3241 attcggcctg ctcatcttag ttccttcctt tacttgtttt tttggtacca ctttgtttca
     3301 gcaaattttt cactgatttc tttgactgac atcacatttt tgataccgat aactgtaact
     3361 ccttttttct ctgcctgagt aaacatattc aaaaaattga ctggcgtaaa aactacatcg
     3421 tattggccag ctgaactttt tccctctgaa atggcacaat gatggatttt ggtaattggt
     3481 acgtttagtt ctttcattgc tttttccaca cttctcatca tcatcaagct tgttcctgat
     3541 ccatttgcac acgatactaa aattctcatc ttaatccctc cattatgagt gttgtctaat
     3601 cagattttct tttatactct ctgcttttct gcatatgtgt catagtcttc tacaatcaaa
     3661 aaataaccat caggattttt ccgatattgt aactgcggaa tagccaataa accaatgaca
     3721 actacggcaa ttccagcata acctaggact ttcatgatta ctgtaaacaa tggccaagct
     3781 gttgcccagt cgaacatccc gatataacca ccgtatgttg acatcccaat ccatgtagcg
     3841 aataatgctg aaccgcctac ttgtatgatt cctgaaagaa acgggaacag gcaagcggct
     3901 ttgaatccac ctcggttatt ggcataaact gctataacgg cattatcaaa gaacaacgga
     3961 atgaaaccgg ctatgacgat cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa
     4021 aactgtccca aagcaccaaa caagaaccca atcgttaccg catttggcga accaaagcca
     4081 aaagtagcag ctacgtcaat tcctggaaca gctcctggta aaatcgtgtt tgagattcct
     4141 tggaaagatt gagttagttc atcgacgaat gtccgaacac ctaattgcaa gatcgccaga
     4201 tataccgcaa aatttaaaga agtcgtcata atgtagaata agaaactctg cccttcttgc
     4261 atgaattgcg cttgaatcaa ataatctttg cctagtacaa tcagaatgat cccaaagaaa
     4321 aatagcatga gtaacgaagt agcaaccata ttctcattga aaatcgacaa gaatcctggt
     4381 aatttgatat cttcaatttt tttgtcttgc ttgattgatc tatttttctt ttcatcccgc
     4441 tttttcatcc actcagctaa ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc
     4501 actgcaaatc cggcaccttc tgtcaattcc tgcgtgattc ctactgttaa gttagaagct
     4561 actgtccaat aacaacctaa aatcagtccc ataaaaatca atgcttgtat cctacctaga
     4621 tcaggaaagc aaaacagtaa aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt
     4681 ccggtagtga aaacggaacg cagtttcgta tatttctgaa aacggactaa taagatattt
     4741 aaaacaaatg caatcagtaa taaaatcatc acatctccaa aggtacggcc aaatgtctcc
     4801 attaatccag catccacagc attttgtcca aaataaggat ctgtaaccat ggcatccaaa
     4861 ttgaaccgtt cctttagtcc aaccaagatt ggccgaaaat tattgaccaa accaccagag
     4921 ccaacagtca aaataaagta accgaccgtt gcctttaaga atccagcgat gcattcatga
     4981 agcgggcgtt tcagcaaaat atagcctaat aacacgataa aaccaatcag gtatgctggc
     5041 tgcgtcaaga tatttgttgc aaaatacgtc caaatgctta acaaaaattc ccccatcttt
     5101 tttctcctct atcaattaat tatattctgc catcacaact tggtaatcat cgatggagtt
     5161 cgtagagatg agacgctcaa ccaatccatc tgtcattaat aattcggata atttttggat
     5221 attttctaga tgttcttctg gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt
     5281 ctcctcattc tccccttcac tttcaaaacg gattggctgt ttcaacttgg taaatgaaat
     5341 agctgttcca aaaacaccat cacttttttc tgaagaatgc ggcattgcta cttgaggtgc
     5401 gatcacaata taaggaccgt atttttctac acaatggatg atctcctcca catacgtttc
     5461 atcaataatt tccttatcga tcaatacaag acagctttcg gcgatagctt cacgccaatc
     5521 cgtgatttct ttatctgggt aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa
     5581 actccccttc tttcttataa aaacgacgga aacggtgtat cattttcaat cgagaagaca
     5641 tcttcaaatc ctcgataaaa attaaactca agatcatctt tgtttgtcgg atagatgaat
     5701 ttcccgccta cttgccagat ataaggttta aatccatact tcaggcgatc ctttttgaat
     5761 ttccaaattt cagtgatctc ttttggatca gccataaaat tagaccaaat atcgtagtgg
     5821 acaggaatca ctacttttgc attcaatgac tccgccatgc gcagcatatc aacagaagta
     5881 actttgtctg ttatccctcg tgggttttca ccataggccc ctaaacagac atcgattttg
     5941 tgttcattac catgtttggc aaataaattg gagtaatgtg aatctcctgc atgataaaga
     6001 ttccctcctg atgtttcgaa taaatagttt actgcaattt gatccatctc ctgaggcatt
     6061 tttcctttta atacctcttc tggatcttca caagtgacca acgctgtccg atcaaacgct
     6121 tctaaagcta ctacattgat gtctttgatt ttgactgcat cacctggctt tactgtaatc
     6181 gttttttctt caggaattcc ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg
     6241 aatgttgctt caggacaatt ttgatgaact gctgcggctg tatggatatc tagatgatcg
     6301 gaatggatat gtgtaacgat taaagcgtct acatttttta tcgcaaaagg atcgattacg
     6361 aaaggctgag tacgtaaatt aggctgcata ttttgaacgc cactcatacg catcatctga
     6421 tggccttttt tcatcatgcc attcccatga ctacgttttc ccgtcccaca ccatagatca
     6481 cataaaatat tcgttccttc atgacttttc agccaaattc ccgtacagcc cagccaccac
     6541 atagcaaaag ttccaggatc cactacttct gtttcgattt cttcattcaa gtatgtgccc
     6601 cactcaggaa atgtttctac taaccacttt tcttttgtta cttcattgac agttttactc
     6661 atttgcttgc tccccccata tcgttgttcc aacttacaag taaatattaa tgtgtatcag
     6721 catccatgaa aaccctttta tatgaccgtt tgttttttat atgattttct ttcagtaatc
     6781 aatgattgtt tcagtataat aaagacagat atgaatgttt aaatgaacaa ctagtcaaat
     6841 aaatccatgg atcaagatgt ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat
     6901 attgaaaaac gtcaaaaaga aattttggaa ctgcttcagc agactgaaca actatcaact
     6961 ttcgaattag ctgaatccct tgatgtatct attagtacga tacgaagaga tttaaattta
     7021 ttagaagaaa aaaacgagat catacgcaat tacggttatt gttcttataa ttacaaaaac
     7081 cagactgatt ttgatcaatc tggtcctgaa aggattaaac aagcgattgc cagagaagct
     7141 agtaaataca ttaacgatta tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa
     7201 acactggatt ttcttcaatc taaccatttg acagtcatta caaacaacgt gaaaatcaat
     7261 tactctccgc acaaagataa ttgtagttat attttaactg gtggagagat gcgctttcca
     7321 aaagaagcac tagtgggcga catcgcatta aatacgattt cttcagtcaa tgcagacatt
     7381 tgtatcattg gctgtagcgg agtcgatgta gtacatgggg tcacaacaaa gatattaaat
     7441 gaatcaacga tcaacgaaat gatggtcaac aagacaatca aatgtaagat tttagtcgct
     7501 gatcatcgaa aaatcggact gacatcaaag ttcaagattg ctgatatttc agtctttgat
     7561 tatttgatca ctgataaatt ttgttctgaa aaagtattag aggagatcag aaatacgggg
     7621 ataaaaacca ttcaagtaga ttcattttaa gaaccaggac gtaaatatgt aaataggatt
     7681 atcaaaaaaa tgaataacaa atttatcgat aaatgaaaaa cacgctacgc tcaaattgat
     7741 aaaggaatcc caagatgtat ccatccaatc gataccatca taatctattg cttcttttat
     7801 aaccgttata tgttgcctaa ataggaatca aacaatatcg aaaaaaggag gtatgacaaa
     7861 agtcacacct ccttttttcg aataaacggt attcaaatca gaacactgat ttcacaacca
     7921 ctttatttca ggtcgttttc aatcaccttt accgcgtaat catttccgtt gaaacgcgct
     7981 attttttttg cgtccatata tctttctaaa taatcatctt ctcctaattg cttatggcaa
     8041 gaagcaattt cataaagcaa gtattccaat gcataagtcg atttatgttt cttattatac
     8101 tcaataccct tctcacataa ttggattgct ttatcataat cctcgatttt acggtaatag
     8161 gtggcgatat cataatatac aatgcagacc atcagattat caaaatcagt ctcactcaat
     8221 attttaagtg tacgctccac ataaactttc gcaaaatcta attctccttt tgacaagtag
     8281 atcattgcca cgtttgcagc tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc
     8341 aacacttgat taaaataaaa taatgctgca tctggttttt tcgcataaag atgatcaagc
     8401 agtccttgat aaaataaaat ttgatttaaa agatcagaat tacttacttt ttcctgatcg
     8461 atcgtttgta aaattttata agattcttgg tattttgata cagaattcaa tgattgaatc
     8521 tcatttattt ttttcctgat tttgtcttct tctgtttcta ctaatatatc ggagatctcg
     8581 acattcagct ttttgcaaag cgcacttaag atattgatgc ctggcgacat attcttgttt
     8641 tcgatattgc tgacagttgc ttgttcacaa atgccttctg ctaattgtac ttgtgttaac
     8701 ccctttttct ttcggtattc ttttagtaat gctccatcta ctttaatcaa aacaatttcc
     8761 t
//
LOCUS       DAPCMU010000110         8725 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPCMU010000110 DAPCMU010000000
VERSION     DAPCMU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.113"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(231..2612)
                     /locus_tag="RD744_002122"
     CDS             complement(231..2612)
                     /locus_tag="RD744_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDU0558106.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2602..3249)
                     /gene="recU"
                     /locus_tag="RD744_002123"
     CDS             complement(2602..3249)
                     /gene="recU"
                     /locus_tag="RD744_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDU0558107.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3313..3852
                     /locus_tag="RD744_002124"
     CDS             3313..3852
                     /locus_tag="RD744_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDU0558108.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3942..4361
                     /gene="gpsB"
                     /locus_tag="RD744_002125"
     CDS             3942..4361
                     /gene="gpsB"
                     /locus_tag="RD744_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDU0558109.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4383..4750
                     /gene="rnpB"
                     /locus_tag="RD744_002126"
     ncRNA           4383..4750
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RD744_002126"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4959..6125
                     /locus_tag="RD744_002127"
     CDS             4959..6125
                     /locus_tag="RD744_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDU0558110.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6171..7667
                     /locus_tag="RD744_002128"
     CDS             6171..7667
                     /locus_tag="RD744_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDU0558111.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7748..8431
                     /locus_tag="RD744_002129"
     CDS             7748..8431
                     /locus_tag="RD744_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDU0558112.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2744 a         1580 c         1851 g         2550 t
ORIGIN      
        1 tgtgtattta gccgatttaa ttataaacaa gtaaatggat aaagtatatg taatgataaa
       61 agaaaaatgg attttctaat ttttaagcta aatgttagat cacacatgta tatgctggat
      121 agtcttataa aaaatcatgt aatacagctt ggtacatgat ttttatttgt agctgttttt
      181 tacatagaca caaaaaagaa accacgcttg tagacgtggt ttctttttta ctatctgttt
      241 ccagaagctg aggtgctgct ggatggtgta tggtcatttg cagcatccgc tccgctagtt
      301 gctggctcac tggaagaagc aggaggagca gatgaagacg gtgtcgagct ttcttcggat
      361 gaagaagaag gaaccgttgt tgaagacgct ggctcgcttg tttctgctgt cgtgctttct
      421 tttgaggact cagcagttga ttcgctttga ctagtagagc tagagctttg agtcgttgtc
      481 tctgttgttg agcttccagg agtctggaca tagctggaag atggaatagt tgttgatggt
      541 gtaattgcat tcgatctagc tgtatattga tctttgtagt acagttcacc gcccacacga
      601 attaatccgc ttggcatctc ccagtctgta tttgtcacgt tagctgaaac atactgcata
      661 agctcgcgat aaacatcaga ggctacgtgt gacgattctg aagtgacagg tgtcattttt
      721 ttattgtaac ctgtccacac ggaaatcgaa taatttggcg tatagcccgc aaataggata
      781 tctggataca ccccagaaga aataccaagt tttgcatact cgtcatctgt gtaattggat
      841 gttccggtct taccggcttg atataaccca gcgatttgcg cattcgtacc agttccttca
      901 gtgatcgtat cttttaaaat gtctgtgatc atataagctg tttctggaga catggctgtt
      961 tttccatctg gctcataagt ttcttctgtt ccgtcttgga agacgatttt attaacatat
     1021 tgaggtttgt aataagtccc tccattggcg aaagccgcat aggctgctgc catttttaag
     1081 gaagaagctc cgtattttgt cccatcttgt tcttccgtat tactagagat cgcattcgat
     1141 tgatggatcg tactgtattc gatcccaaga ttttttaaga aagaagccac tttatctgaa
     1201 ccgacttcat tgaataattt gactgctgga acattacggg ataagtaaag tgcttggcgt
     1261 aaagtaatgg tgcccatata ttgattatcc cagtttccta caggagtcga agtcccttca
     1321 tagttatacg gtgcgtcagt aatagttttt ccagtagaat atttcaaata ttcaaaagct
     1381 ggtccgtaat ctgtgacagg tttcatcgta gaaccaaaat cacgggaagt attcactgct
     1441 aagttgttgc ctaaagttac gtcttctgcg atgtgacgtc cgccaatctg ggcttttact
     1501 ttcccggtat tggtgtcgat aagggtggag gcaacttgca tctcgtcgtc tgggtaagag
     1561 acatactgat ccgtgttgac gatatcatat agtttctttt gggcgtccag atccaaattc
     1621 gtataaatct ctaaaccgtc tgtgtagaca ttcttatctg ttttttcttg tacttcattg
     1681 atgacttctt tgacataatt atcgacgatc ttagtattgt catcggattg agttagttct
     1741 tgcagaccat ccgttaccgg tacattaaca gcttgatcat attctgtttg agagattttt
     1801 tcgttttgaa gcatcgtata caaaacagta tctcgtcttt tcttcgcctg atctggataa
     1861 acataaggat cataggcgga aggggcttga ggcatcccag caagcaatgc tgtttgcggt
     1921 aaagaaagtt ctgataattt cttaccaaag tacatctcag aagctgtctc catcccgtac
     1981 aagccgtttg acatatacac tttatttacg tagtaggtca agatctcttg tttcgatttt
     2041 ttttgttcca atcttacagc catccacgct tcttgggcct tgcgtttcag agtctgatcc
     2101 tcggcactgg tggagaagaa ggaaagtttg atcaattgct gtgtcaaagt acttccgcct
     2161 tgcagtccgc cagaggtaaa gttagagagg gcagaaccga tgatccgaat agggtcaacc
     2221 ccgatgtgtt tataaaaacg gcgatcttct accgacacga tcgcatcttc taatgttttc
     2281 ggtaattcat tcgcagaaat tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg
     2341 ccatcttgtg tatatagttt ggaagatacg gtagcgtcta attttttatc cgtcaattcg
     2401 ggcgcatctt ttgcgtaata ccagaaaagt cctacacctg ctaaaaagag gatacagaaa
     2461 aatgagagta ttcctaaaag gatcttgata aataatcctt ttttatgtgt tccagaggac
     2521 tttccagaac ctttgccaga attcttcttc acactctttt taggagtagg ctgtttctgt
     2581 cgtctagagc ttctagtttg ttcatttgcc attcttgctt tctccttcaa tagataatgt
     2641 tgataaatag aagtcaaccg cgtccaaata aggaatccgc ggagaaatac cagttttgat
     2701 ctctattcct agttcttcga tcaaagataa aggtagtgat ttgcgtcctt tttccttttg
     2761 ggcgtcccag taagcaatca aatgaacact attcaaataa aaacagcgct tcaaagacga
     2821 aaaccataac aagacaaagc agaccccatt ttgttcgagg catgctttca tatgttcgat
     2881 ttgatgggca tggaagtttt taaagggaaa agatgttttg ttcttggttt cttttgcttc
     2941 aaagtccaag taaaagcctc gatagacgcc attatagtct gtagttgagg cttgggtaaa
     3001 gtaggcttcc ttgatcacag cagcacttct cttaggatag tctactttca cgatttggat
     3061 aggggtaggc tttttatgga taaccgccaa cccgcgggaa agataatatt tattactttc
     3121 attgattgct tcttcaaagc gcatcccccg attgccgaat tcagtgatct gatttctttt
     3181 ttcttgaatt ttttcgacgg gagaggcatt cttataataa ggttggccat ttggatagcg
     3241 caataccatc tttaatcaca ctcctaggcc acattatagc aaaaaagcgg atagaaagaa
     3301 aaggaatctg agatggataa gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa
     3361 ctcggtgttt ttaaagaaaa tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa
     3421 gaactccatc agttagcaga agaagggctg gaatgggtac ttgtcagtgg gaatttgggc
     3481 gtcgaactat gggctgtgca aatagtagcg gaattgaaag aagaatatcc ggaactatct
     3541 cttggtattt tgtatccttt ttctgatttt ggtaataact ggaatgaagg gaaccaagaa
     3601 aaaaaacaat tagcagaaca attagcagat tatgtcgaag ctgttagcca tcaaccctac
     3661 caatcaccta gtcagctaaa gaatcacact cgttttatac ttgagcatac agtgggctgt
     3721 ctgctgatct atgatgaaga attccctgga aaaacgaagt atttttttga agatgcacag
     3781 aattaccaag cagatcatcc ctacgaaatt cggctgatca gtatggacga tcttcaaaat
     3841 tttagtgaat aatcgatatt gtgtttgatt tatcaacttt tttcctatac aatgaaaaag
     3901 gtagaaatgt tattacgtaa atgaaaacta gaggtgtaaa tatggcgaat ttggtttata
     3961 gtcctaaaga cattttacaa caagaattca aaacaaaaat gcgcggctat gatcccgttg
     4021 aagttgatga attcttagac aatatcatca aagattatga agcttacagc aaagaactcc
     4081 ttgctttaca agaagaaaac gatcgattga gtgcaaaagt agctcaatta tctaaaactc
     4141 aaggagcagc tcaaacacgc gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca
     4201 attttgatat cttaaaacgc ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg
     4261 atcaacaagc aagtgcagtc aaaccagcac aacctaatcc aaataactac acaaatgcag
     4321 acacaagctt agatgataat gaaaaaacac gtcaatttta atttttgatt aatgaatttg
     4381 cggttttcgg gtaatcgcgg tctgacttga tcaggctgag gaaagtccat gctcgcacag
     4441 gctgtgatgc ctgtagtgtt cgtgcttagc gaaaccataa gctaaggtac tctttggagt
     4501 aacggcagga aaacaagcta aggcttcggc tatgcttaag tatccttgaa agtgccacag
     4561 tgacgaagcg gctggggaaa cccatccggt ggaacgcggt aaacccctcg agcgagcaac
     4621 ccaaacaatg gtaggggcgc tttttgtctg gaagaagaac aggacaaaaa ggcagtataa
     4681 ctgcagatag atggttaccg ctttgttttt tctccctgga aaagcaaagt acagaacatg
     4741 gcttatagaa aaccgcaaac atcagggaat taggtcgtga caaaagtaat gcgttataag
     4801 aaacaagaaa aacatttgaa agctattttt caatacttca gatgaatcgt tcttattaca
     4861 aaacaaactt ttgtatcacc ggttattcgg aatttgagta aaagtgtggg gtaggactga
     4921 tgtcccacct ccttttttta taaataagga gattgtgtat ggaaaaaaac aggaagttca
     4981 acctcgtcgc aactgcggca agcgggctag aagcactggt tggtaaagaa ttaagagatc
     5041 ttggaatcga atgccaagtt gagaacggac gtgcacgttt tgaaggaaca atggaaacga
     5101 tcgctacagc taatttatgg ttacgtacag cagatcgagt aaaaatcgtg gttggggaat
     5161 tcgatgcgta tacgtttgat gaattgtttg aaaacgtaaa agcattgcct tgggaagatt
     5221 ttcttccact agatgcagct tttcctgtag ctggaaaatc gatcaagtcg aaactatata
     5281 gcacacctga ttgccaagcg atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt
     5341 atcaccgtcc agcttctgta ccactgacgg aaactggcgc acattttcag ttggaagtag
     5401 ctttgctgaa agaccatgtg atggtaactc tggatacgac aggaccaagt ttattcaaac
     5461 gtggttatcg cttagaaaaa ggtggagcac cattgaaaga aaacatggca gcagcactta
     5521 ttatgttgac aaattggcga aaagaccgtc cattttatga tcctgtatgt ggttcaggta
     5581 cgctttgtat tgaagcagcc ttaataggcc ataatatcgc accaggattc aatcgtgagt
     5641 ttgcttgcga atcttgggat tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag
     5701 ccgaagaaaa agcagattat gatattgaat tagatattac tggttcagat atcaatggac
     5761 ggatgattga aattgccaaa gccaatgcag aagaaatcgg attaggctct tcgattacct
     5821 ttaagcaaca agcagtcaaa gacttcaaaa cggataaaga atacggggtc atcgtggcta
     5881 atccgcctta cggagaacgt ctaggagaag aagaaactgt tcggaaatta tataaagaaa
     5941 tgggcgatgt attccgtcca ttaaaaacat ggagtaaata tatcttgact agcgacttag
     6001 cgtttgagga attttacgga caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat
     6061 tgcgtacgga tctattccag tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg
     6121 aataatctta ctaattgatt cattcaattg gatgtttgga ggaataggga atgaaagaaa
     6181 aagagttttt acaagaagta aaagaaatca atcttctaca acaagcttta ggaatcctcg
     6241 attgggatac acaaacaggt atgcctgaaa aagctagtgc ataccgaagt gaagtagaca
     6301 gctatcttta cagcatttat ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc
     6361 gctatttttc tgaacatccg gaagaattgt cggaagttgg aactgccgtt tttgccaaag
     6421 taaaagaaga gtatgatttg gaacatcgcg taccggaaga tcttatgaga aaatatacag
     6481 ctgccgtttc ttcagcacac ggacaatggc aaaaagctag agaaacacaa caattttctg
     6541 attttcaaca cgcattgaat caaaatattg aattaacaaa acagttgata ccttactgga
     6601 gaaaagatga aaagacgaat tatgatgttt tgctgaatca atacgaaccc gggatgactg
     6661 tcgaaatcct tgataaggta tttgatcagc ttaaagaagg aattctttcg atccgccgta
     6721 cgctccaaga aaaagggact gaacctgaaa cagacttttt aaatcggaga atgacgaaaa
     6781 aacaacagaa gagatttgta gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg
     6841 gtcgtttaga tgacactgtc cacccattta tgactggaat taatccgaat gatgcacgaa
     6901 tcacgacacg atggaacgaa acagatttca aaatggctgt ttttggagtg atccacgagg
     6961 ctggtcatgg tatgtatgaa caagacatcg atgataaata tgcgtataca ccggtttatg
     7021 aaggtacatc aatgggtatc catgaatcac aatcgttgtt taacgagatc attattggaa
     7081 gtagtcgcag cttctggaaa aagcaatatc ctttctttca agaatgtgca gaaggaacat
     7141 tcgatgatat tccttttgac aaattttatc gttcattgaa ccatacaaaa tcaagtttgg
     7201 ttcgtatcga ggcagacagt ttgacttacc ctctccatat catcattcgt tatgaaatcg
     7261 aaaaaatgat tttcaatgaa ggggtagatg ttgctgaact acctgaaata tggaatcaaa
     7321 aatatgaaga gtatctggga atacgaccaa caaatgatgt ggaaggcatc ctacaagacg
     7381 ttcattggtc aggtgcaagc tttggttatt ttccctctta tgcattaggg tacatgtatg
     7441 cggcacaatt acttcattct ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag
     7501 acgattattc tccaatcaaa aactggatgt cagagcatat ccatcaatat ggtgcatcta
     7561 gaaaaccaaa tcaattaatc atggatgcaa cacatgaacc gcttaatccg caatacctga
     7621 tcgactatat gaaagacctg tattttaatg tatatcaggt aaaataaaat acaatttaca
     7681 aagatagctg aaattcatct aatctataga tgttttcagc tatttactta agaaagaagt
     7741 ggaacgaatg gataaattac aagtgatcgc tgattatagt atggaccaat tagcgcatga
     7801 tcaaacagga catgggagcg atcataccaa aagagttgtc aagttagctg aaaaaatttt
     7861 ggagacggaa ccgcaggcag accgctttgt gacgcttgca gcagcttacc tacatgatac
     7921 gattgatgat aaagtcgtta aaaatgagaa cgaggcaaag caacagcttc gtgtattcct
     7981 tcgctctttg ccaatagtag aggaacagat ctcgatgatt ttttccatta tcgaaaatat
     8041 gtccttttct aaaaatctct ctgaagcggc cgaactatct ctggaaggta agatcgttca
     8101 agacgctgat cgtatcgaag cattaggagc aatcggcatt ttgcgtactg cttatttcgg
     8161 cggtggacat gggcacccga tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa
     8221 agaaaattac cgaaaaggca cgacagtgat caatcatttt tatgaaaaac tgttcttgct
     8281 gtctgataaa atgaatactg attacggata tgaagaagcc aaacgaagag aaaccttcat
     8341 gcgtgaattc ttagaagaat tttttctgga atggtctatt gacgatgaaa attttactcc
     8401 agacaattgg atcaaaaata acaaaaaatg atggaggttg tgacagaagt ggtcagctcc
     8461 aagatatttc ggatcatttt ccgtaaaagt tacttctgga acactgttta ccaagaggtc
     8521 gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt
     8581 ttgatgaata ttgtaacttg tgattcggaa ttggcaggag aacaccgttt attcggaaga
     8641 ataggactgt gacaagactt ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt
     8701 taagaaatat tcagagagta aaatt
//
LOCUS       DAPCMU010000111         8645 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPCMU010000111 DAPCMU010000000
VERSION     DAPCMU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.114"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..469
                     /locus_tag="RD744_002130"
     CDS             <1..469
                     /locus_tag="RD744_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0558113.1"
                     /translation="IQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHLIDVI
                     GNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKYGRKV
                     YESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            562..1724
                     /locus_tag="RD744_002131"
     CDS             join(562..838,838..1724)
                     /locus_tag="RD744_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0558114.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2004..2984
                     /locus_tag="RD744_002132"
     CDS             2004..2984
                     /locus_tag="RD744_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0558115.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3106..4524
                     /locus_tag="RD744_002133"
     CDS             3106..4524
                     /locus_tag="RD744_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDU0558116.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4595..6064
                     /locus_tag="RD744_002134"
     CDS             4595..6064
                     /locus_tag="RD744_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0558117.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6074..6541
                     /locus_tag="RD744_002135"
     CDS             6074..6541
                     /locus_tag="RD744_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0558118.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6577..7449
                     /locus_tag="RD744_002136"
     CDS             6577..7449
                     /locus_tag="RD744_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0558119.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7530..>8645
                     /locus_tag="RD744_002137"
     CDS             7530..>8645
                     /locus_tag="RD744_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0558120.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQL"
BASE COUNT         2985 a         1299 c         1748 g         2613 t
ORIGIN      
        1 aattcagttt aatcaagtta tagaagaatt catcaaagta aataatttaa tgacccatat
       61 tcaaaaacga ccaatcagaa taactaatga tctaaaactc agtacgagta tgattcattt
      121 gattgatgtg attggaaatt atcctgaaac tagtattacg gaattagctg atcgattagg
      181 cgttaccaaa ggagctgtat cccaacagat tcccacttta gagaaaatga atcttataag
      241 catttctcaa aaaaaaggaa ataagaaaac taaacttgta tcgttaacaa aatatggtcg
      301 aaaggtgtac gaatcacata attatttaca tgaagagctt tatagctcaa taaatgaagc
      361 gttagaatca ttctcaccag accaaatttc aaccattaaa cagattctag aaaaagtatc
      421 ttatacaatc aatgaatatc aagtaagtct gacaagaaag gaagattgac atgaaaaaaa
      481 tgatttttga atcggctaca ctagactaga cagaaaaaat aaggtgtgta aaataaatga
      541 aaacacactg gaggaatttt tatgtcaaga cgtcaacgta gaacctattc aaaagaattc
      601 aaacaacaag tcgtcgatct ttatctcgca ggaaagcccc gagctgaaat cattcgagat
      661 tatgagctga ctacctcggc ctttgataag tgggtcaagc aagcgcaaac tactggctcg
      721 tttcaggaaa aagataactt gatccctgaa caagtagaac taattgcatt acgaaagaaa
      781 aataaacagc ttgaaatgga gaatgacatt ttaaagcaag cggcgctgat attgggacga
      841 aaagacaagt aattgatacc aataagcaca aatactcaat atcagcgatg tgtaaagtgt
      901 taaacatctc ttgccaaacc tattattatc aagccaaacc agttgaagat gaatcagaac
      961 tggaagaact cgttcaagaa gaatttattc gcaatcgaaa agcttatggt accaggaagc
     1021 tgaagcagtg cttggccaat ctaggtcttc aagtgagtca acgcagaatc ggccgtatga
     1081 tgaaacagcg tggcttgcgt tcaacataca cattggctca tttcaaattc cattcttcta
     1141 cttgtaacga agccaagacc gccaatattc tagacggaac cttcctacag gaaaagtcat
     1201 tagaagcgat tgtaaccgac cttacctatg ttcgtgttgg gaagaaatgg cagtatatct
     1261 gcctgattct tgatttgttt aatcgagaaa tcatcggcta ttcatgcggg gagaagaaag
     1321 atgcgacgtt agtcaaggaa gcttttgcaa agattccgta ctcgttgaat aatgtacaac
     1381 tttttcacac ggatcgtgga aaagaattcg ataaccagac gattgacgaa attttatggg
     1441 cctttaatat cacgcgttcc ttaagcaaaa aaggctgccc ctatgataac gccgttgcag
     1501 aatccaccta taaatctgtc aaagtagaat tcgttcatca ataccgcttt gagacattgg
     1561 ctaatttgcg attagagcta tttgattacg tccattggtg gaataccatc cggctgcacg
     1621 gcacgttagg ctatgagaca ccgatcagtt ttcgacagca gagattggcg cagcgaatcc
     1681 ttgataatga gctcggatgt gataccggtg gcgaggcacc ttaattattt gaatgagctt
     1741 ctgccttaga gaatcagatt cgaggaggct cgttgtcaag ggcagtcgga acaccagcag
     1801 caagcttacg caatacctta taatttttgt caaaaaaagt gttgccattc cacatatatc
     1861 tttaaacagc tattgccaga taaaaataaa tgatattata tatataaata cttatccgaa
     1921 aaggggaatg agagagtcaa ggttcttgga atgtcgaata gtcgagtagg tagattttct
     1981 atctacaacc ttgttgttta aaaatgaaaa aaattggaat tagagaagtt gcgaaagagg
     2041 caaatgtttc tcctacgact gtgtcgaggg tattgaatga tcgtggatat ttgagtgagg
     2101 aaacaaaaag aaaagttttt gatgccatga aaaaattaga atactatcct aatgaactgg
     2161 cgagagcact ttttaaaaac gaaacatata ccgtaggttt gatttttcct acaattacta
     2221 atccatttca cgctgagtta attcaagata tcgagttcga gctttccagc aggggatata
     2281 aagtgttgct gtgcaatagc ttaaacaatc caacaaaaga aaaagagtac ttgacgatgt
     2341 tgagaaagaa tcaggttgat ggaattatag taggaacgca caataaagat attgcggatt
     2401 atgatattcc agggctacct attgtagcga ttgatagaag tcttggtgaa aatacagtta
     2461 cagtatcttg tgataattac aggggtgggg aattagctac tcaactactt gtaaatcatg
     2521 gctgtcagaa tatactgtgt attcggggag atagtaagat aaagctaccc gcgaatagta
     2581 ggactttagc atataaagat gttttgaata aattcatgct ggaaccgtta gtcctagaag
     2641 taccatttgt aaaagaaatc aatgaaaaaa atgaaataat taagacatat ctggaaactc
     2701 atccaaatat tgacggggtt tttgcaggag atgatttatt ggcttctttg gcgattaaca
     2761 acttaggaag ttcgggcaaa agagttccaa aagaggttaa agtgattgga tttgatggag
     2821 ctcaacaaac attgatttat aatccagaat tatcaactat tcaacagcct atagatcaaa
     2881 tatctaaagt agcagtaaaa aaattattga atatgattaa gggcgaaaag gagacggatg
     2941 aactttcact tccagtaaag ctcataaaaa aaaatacagc ataagttaaa tcggcgaaaa
     3001 attccttcgt cgatttaaaa atatttttat gaaacgggtt gacatatgaa acgggttgac
     3061 atatattagt tgtgaagata aaaacctttt cactggagga agaaaatgga gaaattatta
     3121 attaaaagca ataatcatca actttactac caacctaagg atgtttgggt cggagatgtt
     3181 atgccttacg ggaaagaagg aaaattcttt atttatcatc aacgtgatac tagaaatcca
     3241 gttccttttg gagaaccgtt tggctgggct ttagcaacta cgaaagattt tgttcatttt
     3301 gaagactttg gggaatctct tttaagaggg gatgacaatg agagagacca gtttatctac
     3361 gctggttctg tatttgaggc aaatggaaag gttcacgcat tttatacagg gtataatcgt
     3421 gaattcttag cgcagggaaa gacatctcaa gtgttgcttc atgctgagag tgatgatttt
     3481 gttcattggg aaaaatcaga aacagcattg gagttagaac cacaagaagg gtatgatatt
     3541 cgaaactgga gagatccatt tgtcgtttgg gatgatgaaa aagaagaata tttgctaatt
     3601 ttaggggcgc gaaagggtca ggataaacat ctacaaaccg gacgtttagt ggaatttaca
     3661 tcaaaagatt taactaattg ggaattcaaa ggtgattttt gggctccggg attgtatacc
     3721 atgtttgaaa tgccagaact tttcaagatt ggtgactggt ggtatcttgt ctattcagaa
     3781 tactcagatg gaaataaaat actctatcga atgagtcaaa gtttggaagg tccttggatt
     3841 aagcctaaag atgaagcttt tgatggacgg gcttattatg caggtagaac agcttatgat
     3901 ggaaaacgtc gagtcctatt tggatgggta ccaacaaaag atcaagaaaa tgataaaaac
     3961 aattatttat ggggaggtac tttggttcct catgaagtct atcaaaaaga ggatttttca
     4021 cttggggtaa aacccataga cgaagtgtgg aatgcattca taaatttaca tgatattgcc
     4081 gatgtagaga tagaaacagc agacacaaga gaagaagtaa cgcttgtcaa aaatagtgga
     4141 actttgttta atttagaaac aacaatttca tttgatgaga atcttagtga cttttcactg
     4201 agattatata aaaaccttga gacagatgaa tcttacgaat atcgcttcaa tttagatgag
     4261 gggcgtctta catttgataa aaacccttgt tacccttggt ttcaatatat ggataaagga
     4321 cttattagac ctatatcttt aaagcctaac caatcttata aactaaaagt gattgttgat
     4381 gacaatataa tgacacttta tattgatgga atcgctctta acgctcgtgt acatggttgt
     4441 tttggtcaag caattactat tacagcaaca aatggatcaa tgactgcaat aaataccaag
     4501 ttttcaaatg aaataaagaa ataataaacg tcaatcatgt ggcaggtctg aaaatcaaac
     4561 atgacctgtc acatttaaaa gggaggataa atgaatggac tacaaaaaaa ttgccgaaga
     4621 tgtattggaa aacgttggag gaaaagagaa tatctcaagc gctacacact gcattactcg
     4681 tttacgttta attcttaaag acaaaagcaa agcgaatatg gaaatgcttg aggaaataga
     4741 aggtgcaaaa ggtgttcttt tcaatagtgg acagttacaa gtaattttcg gaacaggtat
     4801 aattgaaaag gtttacaaag agttcgttga attgacagga gctaaggaag tatccgtttc
     4861 tgaaatgaaa gacgaaggag tcaataattc aggaaataga cttcaacgag cttttaaagt
     4921 tttctcggat atctttattc cgatcattcc agcttttatt ggggcagcaa tggttttagg
     4981 aattcgttcg ttgattacca ctcctggaat gtttggaatg gatggctcat tcgctgatca
     5041 caatgtattc ctaaatgatt tatccaaatt tctgggtatt attgcaacca catttgatta
     5101 tttaccagtt ttagttatgt attctgcaac aaaacgtttt ggcggaaacc ctattttagg
     5161 attacttgta ggatttgtta tgattcatcc aggactagcc aatcggaatg atttcgtcat
     5221 gggaactata aagcctgatt attggcattt atttggatta agcattccag cagttgcgtt
     5281 tcaagggggt gtttttccag caattttgac ttcttggttt ttagcaaaag ttgagaaaat
     5341 tacaaataaa tattctcctc aagtacttgc ttacatttta gtcccaactg tgacaattgt
     5401 tcttgccaac tttgcactat ttttagtgtt tggtccattg gggaatttta tcggtaatgt
     5461 tttaggtgga atcattgatt tcctatatat gaagatgggt gcctttggtg cttttgtttt
     5521 tgcaggcgcg ttacaaccat tagttgtaac aggaacacaa catgctattc aaggtattga
     5581 ggcaaactta gtcgcaacaa ctggattcaa ttatatccaa cctatttggt cagtttcaat
     5641 tattgcccaa ggtggcggtt gtattggtat gtatttactc tttaagaaaa aatctaaaga
     5701 tagagatatt gcgatttcaa gctttattcc aacactggta ggtattactg aaccggctat
     5761 ctttgctgtt aatttgaaat attcaatcgt tccttttgtt tgctcgttta ttggtgctgg
     5821 ttttggtgga gcatttatga aaatttttga tgtaaaagcc attggacaag gattgactgt
     5881 attgccaggt ctgactattg ctaatccagt tcttccttat atatttggta atctcatagc
     5941 attcatttta ccaatcattt ttattctcgc ttatgataaa tataaagtgt ttatccctga
     6001 agctaaacac cgtaaatttg cagaaaaaac tgactcaaaa aaattgccag ttcaagtaaa
     6061 gtgagatgag agcatgttaa atttttttag aaaaaatgaa ataagcgttg tagcacctgc
     6121 aaccggtgtt tttgaaaaac tggagaaatt aagtgatcca gtcttttcaa aaggaatgat
     6181 gggacaagga tttgcaattg atgcaaaaaa cgaaacgatt gtatctccaa tcgatggttt
     6241 tgtttcaagt atttttccta caaaacatgc cttaggaata aaaacaaaat caggaattga
     6301 gatacttgtt catgtaggaa tagatacagt tgagctaaat ggtgagggat ttgatattaa
     6361 agttcaagaa ggacaaaaag ttaaggctgg ggatattctt atgaccatta attttgtagt
     6421 ttttgaaaag aatcacaaga ctaaagatgt cattgttgtt tttccagact accaagacga
     6481 aattttcatt ccgaaacttg aagaagtaaa acaaggacaa gaattgtttg ttcttggcta
     6541 atgataagta attaataata gaatcgagga aaatgtatgg tttttggggc tattgaagct
     6601 ggtggaacaa aatttgtttg tgccattgga gataaagatt tgaacatatt tgaaaaagtc
     6661 agctttccca cgactacacc caaagaaaca atgaatttag ttttagattt ttttaatcat
     6721 cataaagatg atttagaagg tatcggagtg ggatcgttcg gtcctattga tattcattgt
     6781 gaatctaaaa cgtatggctt cattacatca acgccaaaac ttgcttggca ggattttgat
     6841 tttattggaa gtttaaaaaa gcaacttaat attccaactg cttggacgac tgatgtaaat
     6901 gcctcttgct atggtgaata tgtttctgga aatggcagag gcctttcaag tgttgtttac
     6961 tataccgtag gaacgggcat aggaggtggc gctatccaaa acggaaactt cattgaaggt
     7021 tttagtcatc cagaaatggg acatatgctt gtaaaacgac acaataaaga caacttttct
     7081 gggacatgtc cattccacgg agactgttta gagggcttag ctgctggtcc ggcaatcgaa
     7141 agaagatcag ggaaaaaggg acaagaagtg actgaaaatg acgttacttg ggaaatagaa
     7201 gcttattaca ttgctcagtg tgcatataat acaacattga tgttatcacc tgatgtcatt
     7261 atatttggtg gcggagtaat gaaacaaaaa cacttaaaaa agaaagtaca agataagttt
     7321 attgagttga tcaacggata tgtcgctatt cccgatgttg acaattatat tatgacacca
     7381 aaattgttag ataattctgg tattgtaggt tgtttagctt tagcaagaga cgttaaagaa
     7441 aaatcttaat ttggaaaaaa gatcgtctcg actatttgtt tggttgatgc gatctttttt
     7501 tcaattagta attgaaggga cgcctaaaag tgaaaaaaaa aatgaaagat gaagtattag
     7561 agtatatcaa aaataattta aagtattacg atttttctgc tcaagatatt gcaatgaaat
     7621 tttgtattaa aagaaatgtc gcttcccact atttgaatca gttattttca gatggaaaat
     7681 tactaaagaa tgattctgtt agaccagtaa tgttcaagta taatcaacaa aagccaaaag
     7741 actgcttttc taagtttatt ggagcagaca tatctttgaa atccactata gataaatgca
     7801 aagctactgt aatgtatccc cctaatggcc tcccattaat aataaaagga aacagtggcg
     7861 ttggaaaaag ttttttagca agtttgatct atcagtatgc tcttgataga aaagtaattc
     7921 ataatgacgc aaaatttgtt gttgtgaatt gtgcagatta tgcaaataat ccagagctat
     7981 tgtcagcagt tttatttgga tataaaaaag gagcctttac tggggctgaa aaagatactg
     8041 cagggatgct atcttctgct aatggaggat accttttttt agatgaggtt cacaatctaa
     8101 gtgcagaaaa ccaagagaaa ttgtttcttc ttatggattc tcaaaagtat cgtaaacttg
     8161 gtgaaagtgt gcaatgggag tatgcaaatg ttcgccttat cttagccaca acagaagata
     8221 aaaatacctc tttgttagca acttttagaa gacgcatccc agctgaaatc actctgccag
     8281 attacaataa tagaagtaca tctgaaaaaa ttcaattatt atttgaattt cttaaagatg
     8341 aagcaattac gattgattca aaaatatttt gttcgattga actttttact gatcttttat
     8401 cgaaaacatt tgaaggaaat gttggagaat tgaggaatga aatcaagctt ctttgtgcag
     8461 aaggttattt aggaaataag agaaagtctt caatttattt aggggacaaa ctagacagcg
     8521 gtttttggat agatccagaa attagtatag atttaaaaaa aatgtttctt aaatcacttt
     8581 ctgaaattga tttgattgaa ctttttcaaa atcacatttc aattgaagaa agcatgagtc
     8641 aatta
//
LOCUS       DAPCMU010000112         8572 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPCMU010000112 DAPCMU010000000
VERSION     DAPCMU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.115"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="RD744_002138"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="RD744_002138"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDU0558121.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="RD744_002139"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="RD744_002139"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDU0558122.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="RD744_002140"
     CDS             complement(3070..4323)
                     /locus_tag="RD744_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDU0558123.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="RD744_002141"
     CDS             complement(4329..5252)
                     /locus_tag="RD744_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDU0558124.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="RD744_002142"
     CDS             complement(5473..7965)
                     /locus_tag="RD744_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDU0558125.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="RD744_002143"
     CDS             complement(7972..8439)
                     /locus_tag="RD744_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDU0558126.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAPCMU010000113         8494 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPCMU010000113 DAPCMU010000000
VERSION     DAPCMU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.116"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..173)
                     /locus_tag="RD744_002144"
     CDS             complement(<1..173)
                     /locus_tag="RD744_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558127.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="RD744_002145"
     CDS             complement(593..1714)
                     /locus_tag="RD744_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDU0558128.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="RD744_002146"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="RD744_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDU0558129.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="RD744_002147"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="RD744_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDU0558130.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="RD744_002148"
     CDS             complement(4304..5215)
                     /locus_tag="RD744_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0558131.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="RD744_002149"
     CDS             complement(5232..6239)
                     /locus_tag="RD744_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDU0558132.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="RD744_002150"
     CDS             complement(6236..8365)
                     /locus_tag="RD744_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDU0558133.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="RD744_002151"
     CDS             complement(8370..>8494)
                     /locus_tag="RD744_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0558134.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1487 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga gacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAPCMU010000114         8428 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPCMU010000114 DAPCMU010000000
VERSION     DAPCMU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.117"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..1385)
                     /locus_tag="RD744_002152"
     CDS             complement(<1..1385)
                     /locus_tag="RD744_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDU0558135.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFT"
     gene            complement(2134..2757)
                     /locus_tag="RD744_002153"
     CDS             complement(2134..2757)
                     /locus_tag="RD744_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558136.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(2916..4313)
                     /locus_tag="RD744_002154"
     CDS             complement(2916..4313)
                     /locus_tag="RD744_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0558137.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(4345..5316)
                     /locus_tag="RD744_002155"
     CDS             complement(4345..5316)
                     /locus_tag="RD744_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0558138.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            5690..6115
                     /locus_tag="RD744_002156"
     CDS             5690..6115
                     /locus_tag="RD744_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558139.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(6603..7304)
                     /locus_tag="RD744_002157"
     CDS             complement(6603..7304)
                     /locus_tag="RD744_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDU0558140.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(7320..7760)
                     /locus_tag="RD744_002158"
     CDS             complement(7320..7760)
                     /locus_tag="RD744_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDU0558141.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(7760..8335)
                     /locus_tag="RD744_002159"
     CDS             complement(7760..8335)
                     /locus_tag="RD744_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDU0558142.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         2447 a         1739 c         1387 g         2855 t
ORIGIN      
        1 gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact
       61 gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc
      121 gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat
      181 tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat
      241 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
      301 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
      361 ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag
      421 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
      481 tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta
      541 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
      601 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
      661 ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca
      721 tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg
      781 attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg
      841 ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg
      901 atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa
      961 accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc
     1021 aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg
     1081 actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta
     1141 tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa
     1201 gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg
     1261 atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag
     1321 aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt
     1381 gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa
     1441 atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc
     1501 acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt
     1561 cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa
     1621 aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt
     1681 ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
     1741 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
     1801 aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt
     1861 caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa
     1921 taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca
     1981 gagttaaacg gcaggctcaa gacctcttga tacacggtgt tcaatcagct gaccttcggt
     2041 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
     2101 aggtcaaaac gctgatttca caacctctgt ttttcattgt ccataggatt gatattgtcc
     2161 ttgtgatgac tcattcgtaa taattgaggc ttgattttct tcacttcccg ttatcgtttg
     2221 tccactgcca gaatctgaag gacttgttcc tgtcgactcg tttttttgag aagagacagt
     2281 tgttttcttg acttgttcat ttgtcttttt ccattccgct aaacgatcgg cgaccacttt
     2341 tttgtctact ttttcagaat aagcggtgat cacttttcca tttttcaaac gggctacttt
     2401 gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg tctttaaatt gatattgtct
     2461 gagagaaatc gcactaagta cgtcatcgat attaataaat tgattttctt cttttccttt
     2521 tggcaaataa atgtccaatt cgcctttttc gtaattggca gttattttat actccgccca
     2581 ccaataagtt gagaattctt ttttgaaatt atctactgat tctttgcttg ctacgatttt
     2641 ttctgtttct ttctgagcaa gatctgctgt catttttcct gaaaatgctg cggataaaat
     2701 aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac ggtctttttt tcctcatgct
     2761 ttcctccact cctatttaat tttgactatt ctattatata caaaaaatga aatagaaaga
     2821 caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg
     2881 gagaatttca cgcatttgct ctcaactatt ctttcttaag cctcaaatcc gtttttatct
     2941 gatacgtctt tgaaccaatg acccgatttt ttgattgttc tttttccatc ttgtgctaag
     3001 tctactgaaa taaagccata tcgattctta tatgcattgg tccacgacca gttatccata
     3061 catgtccaca tatgatagcc taaacaattg gcaccttctt gaatggcttg atggacatat
     3121 ttcagatgag attggacaaa ttcgatccga taatcatcgt ggatcatgcc atcttctttg
     3181 ataaatcgtg cttcgttttc tacacccata ccgttttcag aaataaagca gtcgatattt
     3241 ccataatgat ctcgtacatt gatcaaaata tcatagattc cttgttcata gatctcccat
     3301 ccgcgataag gattcatttt acgaccaggc atttcataat tgtcgaaata atcctcaggc
     3361 attggaccgt ttttagtatc gattggtgtt tcttttgctt ttacccggcg tggctggtaa
     3421 taattgatac ctaaaagatc tactgtattt tctttgataa ccgtcaaatc ctctgcatgg
     3481 atcacaggca tgagatcaag ttcttttact atctcaatca attcacttgg aaactcgcct
     3541 tttacagctg gatcaaggaa tgaacgattg aagaacgcat ctgctatttg cgctgcttta
     3601 acatcttcag ggtttgattc gtctcttgga tagcttggtg tcagattcaa gatgatacca
     3661 ataacaccac cttgctccat ttcatggtaa gatttgatgg ctaacgctga agccaacgct
     3721 tcatgatagc caacttggat cgcttttttc atatctgact cttctggata atgccatcga
     3781 tataaataac ctgcttcgac tggaacgatt ggttcattat gagtaaacca tttttttact
     3841 ttcgaaccaa acaattcaaa acaacttttg gcatattttt cataggcttc aaccgtttct
     3901 cggttcaacc atccgccttt ttcttgcaga tacatcggca tatcgaaatg atacagattc
     3961 acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg
     4021 gcttcttcgt tgattgttcc agattcatca ggtatcaaac ggctccattg gatagatgtt
     4081 cgaaatgaat tatgcccagt ctgtttcatc aactgtacat cctcttgata cttcgtataa
     4141 acttgcgagg ttttttcagg tcctacttga tcaaagaact tttgcggttc ttttttgtac
     4201 caataatcaa aaatgttctc tccttttcca tcgcctgaaa tccttccttc tgtttgtggg
     4261 ccacttgctg cagaacccca ccaaaaattt gctggaaatt gatatttttt catcaaagca
     4321 cctctttttc ttttttagtt cgttttagtt gattccatgt atgatatttt ccatcacaat
     4381 cactttttca ttttcttcct cttctttttt gattcgtttt aacagctgtt ctgctgctcg
     4441 ttccccgatt ttatctgttg gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt
     4501 tttatctaaa taatcatagt gactgaacgc aatatccttc ccgattctga caccttcttg
     4561 attgaaacat tcgatggcac caatcgacat attgtaattg ctgacaaaaa tagcagttgc
     4621 ccctttttct aataatgctt ttgccccgta atagccgctt tttctagaat agtctcctgt
     4681 aaagacctct aattggatcg atggatcatt ttggaccgct tctttggctc ctgctaaacg
     4741 ttctcttgct acgtagtctg tttgagtggc agcaataaat ccgatttttt tatgtccttg
     4801 ttcaccatga tgagcgatca cttttgctac actttctcga tcattcacta atattgtatc
     4861 aatacctgct ggtccttctg gacaattcaa tgcgataact ggaatactca tttttgtgag
     4921 catttcaaat ccagaccact cttcttcgga taagaagaca atcagcccat cgatcacatg
     4981 cttcatcagg tattcaattt tttgttcaat tttttctggc tgatcggcaa acccgcttaa
     5041 taataagcta tacccatttt cttcagctac tctttcgatc cctgagacaa cttccgaccc
     5101 aaaacggctg gaaatcccgt ttatcaaaag tccaatagag aagcttcgat tattcttcaa
     5161 ccctttagca atgatgtttt ctttatagtc caacgctgaa atcgcttctg ctattttttt
     5221 catgttcttt tccttcagtt gctgaccatt caagtaacgg gaaactgttc ccactgaaac
     5281 accagccatc ttagccacat cacggatcga tgccatacat tctcactcct gccagttaca
     5341 tttgatttct ctttcttgtt gttcgattat gatgaccgca ttctttgcat agtgttgctt
     5401 gtcatcaata tccatataac taaagtcttc agtagagcaa cttcctctca ctattaatta
     5461 tacttaaaca agtatttgaa ccggttcatt tttaatatag cattttttta aaagattgca
     5521 acactttttt aaaacgcttt ctgaaagtgg cggatttttt gcctttttaa aattttattc
     5581 taaaatttac tctatttgtg ggtgttttgt gcgtttttct cttccatccg tttgaagttt
     5641 tacattccaa tggtataatt tctttatcct atcaaatggg ggaagtattt tgaagaaatt
     5701 aatcgtacta tttattttgt ctgcgggact tttgacattg actggctgtg atttgtttgg
     5761 cggctctgac caatcctcgg acgaaacaac cgattcttct gtcttaaaag cggaaaaagc
     5821 agataccacc aacagtgtat atacgctaaa agtacgtaaa gatatggatt tatccgcggg
     5881 acagttgaca tttacagata aaaaaatgga atggacacgc acgtatcaga cacaaggaac
     5941 tgattcttct gaatcatctg aagaaaaaac agtgctgaca gatatcaaga tcactaccaa
     6001 agatgacgaa tatattattt caggaaaaga aaataaaaaa gaaacaacag tcatatttac
     6061 aaaaatcggt aattaccgtt tgaaggatga ggatggcaac atctacagtc tatagtgccg
     6121 tctgttggat cttcttttgt ttctcccctt cttttctgct atagtaaaga agcaagatga
     6181 ttgtaaagga gacgaaatgg atgatcgata gtcctgatta ttgtacatgt aataacaaca
     6241 aaaatatcca tacagaattt aatgaccaag gaagcttgag catatgcgac aactgtcaca
     6301 aactagtcaa agattcattt aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa
     6361 gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa
     6421 actcagcttc ttcctttgat caagcttcat cagtaaaaca aacagtggat catttcagtt
     6481 tacaaatcaa agagtaaaga tttatttatt tcaaatattt gaatataaaa taaccaaacg
     6541 atagaaaggc ttattctaca tcacgtagta atttttcttt cccgtcgttt ggttttttat
     6601 ttttagccta attcttgacc ttttgcatgc tggttatccg taaaatattt tccagcaggc
     6661 tttccttcca attcagctaa tttttcttgg atctgacgga tcaccttttt tgcatcttct
     6721 ggattttcta caaaatcgaa tttatcccca tccacttgta ttttaggaca tacatcaaaa
     6781 tcttcatacc attgattata gcgggtgttc aacgtttcat aataggtata taacgtctca
     6841 tcgctttcta gctgttcata gtcacgccca cgttttttga tacgttctag catcgtgtcc
     6901 aaagaaactt tgatatggac catcaaatca ggccgttttt ttggagcaac atcatctaac
     6961 tcgtttagca tcgtatctaa taagctatcg tactgctgta cttcgatatc cgttacccga
     7021 cctaaatcag cgtttagatg gaacaacagg ctatcttcat agatggatcg gtctagcaca
     7081 ttgtcatcgt tcactaaagc atttttcatt gctaaaaatc gcttgttcaa aaagaagatt
     7141 tgcaataaaa aagcgtactg ctcaggattt gaataaaaga gcggcaatac ttcgttatct
     7201 tcgacatttt cataaaatgg tttagaatca atctcttttg caagcatatc cgtcaaactg
     7261 ctcttacctg caccaatcgt tcctgaaatc aaaatcactg acatacaacc cttcctcttc
     7321 tataagtact cgcctacgta agtttcaaca ggcaatgttt caaaatcata ttcacgtaca
     7381 gcttggtctg attttaaata cgtatggaga ctttgtgtaa tcatcaagtt cgtaggtacc
     7441 gtctcttctt gtacaatgat catcggtttt ttcaacatag ttgccacacc taattcgtaa
     7501 gccgttcctg gatcgattgt ttgatgctca aagtcaagga tagcaacaac aaattctgca
     7561 tttgtcagtt cttccatatc tttttcgtat acttcctgcg cccatccagc gctgaatagt
     7621 tcataattgc tttcttggtg atgacgtgga gagtagaaac ttgtgactgt tttgttttcc
     7681 tccaacgctt tttcgatacg acttactcga tcgatttgct cctctgaaaa aaatggtcct
     7741 gctaaatata tttgactcat tattttgcct ccttcaataa taactgattt ttaatagata
     7801 ctgctcgtaa ttcattgacc agttccaatg gattgttgga aaagattact tgatccaacc
     7861 attttttatc cataaaatcc tcttttgcaa aagtttctaa taattgttgc aagccgctgt
     7921 aataaccgcc aatattcaat attccgattg gcttgtcggg cgtcagaccg attgctatcc
     7981 aagaaagaag ttgtgagaat tcctccaatg tacctagacc tccagggaaa acgaggacag
     8041 catctccctt ttcaaataat aatacctttc tttcatcgat cgtttctgta ggaataaatg
     8101 tagtaacatc ttttccaggc aattcagttt caaaaagccc attcggatag atacctgtga
     8161 cttttccgtt attttccagt actgttttac taatcactcc cataatcccg gagtatgaac
     8221 caccgtaaac cagttcgatt tcttcttgtg ccatgtattt tcctaacgtt tgcgcaataa
     8281 atttgtaact ttctttatta ccaaaacggg aaccgcaaaa aacagtaagt ttcataaaaa
     8341 aacactccct aactcaaaag tctatcttca ttctacacgt gtttttttaa aataaaagac
     8401 tagattttgt agttttttag tggtatat
//
LOCUS       DAPCMU010000115         8382 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPCMU010000115 DAPCMU010000000
VERSION     DAPCMU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.118"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            62..2671
                     /gene="clpB"
                     /locus_tag="RD744_002160"
     CDS             62..2671
                     /gene="clpB"
                     /locus_tag="RD744_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDU0558143.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2822..3508)
                     /locus_tag="RD744_002161"
     CDS             complement(2822..3508)
                     /locus_tag="RD744_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558144.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3486..3980)
                     /locus_tag="RD744_002162"
     CDS             complement(3486..3980)
                     /locus_tag="RD744_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDU0558145.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4000..5220)
                     /locus_tag="RD744_002163"
     CDS             complement(4000..5220)
                     /locus_tag="RD744_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558146.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5367..7202
                     /gene="lepA"
                     /locus_tag="RD744_002164"
     CDS             5367..7202
                     /gene="lepA"
                     /locus_tag="RD744_002164"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDU0558147.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7296..>8382)
                     /locus_tag="RD744_002165"
     CDS             complement(7296..>8382)
                     /locus_tag="RD744_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0558148.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2688 a         1608 c         1688 g         2398 t
ORIGIN      
        1 tagtttttgt aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg
       61 tatgaatatt gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat
      121 tgcggtcact cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca
      181 acctaatcat tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga
      241 acatgaaatt gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata
      301 tggacaaaac ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga
      361 aagcttcggc gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa
      421 aaattatccg ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa
      481 aaacatcgaa gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata
      541 caaagcatta gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga
      601 tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac
      661 aaaaaataat cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg
      721 gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt
      781 ttcattagat atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag
      841 actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga
      901 tgaaatccac aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct
      961 gttgaaacca atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga
     1021 gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt
     1081 caaagaacca acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga
     1141 gatccaccat ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga
     1201 ccgttatatt actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag
     1261 tgcaacgatt cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg
     1321 tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa
     1381 aaaacgcttg aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat
     1441 gaagatgcag tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga
     1501 aatcgataaa gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc
     1561 tgctgtattg cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc
     1621 taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc
     1681 tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga
     1741 aaaactaatt aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt
     1801 tgatgcggtc agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc
     1861 tctaggttct ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc
     1921 gttagctgag aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata
     1981 tatggaaaaa catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga
     2041 agaaggtggt caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga
     2101 tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg
     2161 acgcttgact gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac
     2221 cagcaatatc ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga
     2281 agcagtggct gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa
     2341 ccgaatcgac gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt
     2401 tgacaaaatg gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat
     2461 cagtgatgaa gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag
     2521 accattgaaa cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc
     2581 aggacatgtt atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt
     2641 caaaacagaa gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag
     2701 gggtctgaca caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag
     2761 tttcttcgaa aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt
     2821 attaggtatt tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac
     2881 tttgagacca tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc
     2941 ccatcgtatc catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac
     3001 gttgaagatt catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc
     3061 cgcaaagtcc agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata
     3121 tctcagcatc tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg
     3181 gactcaccgc atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg
     3241 tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta
     3301 ttttaagttt gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata
     3361 aagattccac gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat
     3421 aaaaattata gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga
     3481 tttctttaat cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca
     3541 atttctgaag aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg
     3601 tagtgaaatt gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg
     3661 ccttgctgtc gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca
     3721 ccgatttccg atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa
     3781 tcactagcca acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca
     3841 gttttgtcat tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc
     3901 tctctgattt ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt
     3961 gcggcaaaat aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta
     4021 agcttccatg tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa
     4081 ccgttcgatt cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt
     4141 ttggatatcc agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg
     4201 ttcatcccat gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat
     4261 gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc
     4321 aacgatccga gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa
     4381 aaatgctaaa ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg
     4441 tgcggaaata aagttccata agccatttcg agcttcttga tcaagataga acatttcttt
     4501 tacgtgaaat atttcttcag ctacccaata gaacagaaca cctcttggtt catggttgct
     4561 atcgtagtag acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca
     4621 atgaaaatca ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga
     4681 aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt
     4741 aggtagctgt gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt
     4801 acgccgatag taggggatac tgtaaggata caaataagag atccattgct tattcttgcg
     4861 catattatcc aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg
     4921 ataagttcca actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc
     4981 acaaggatag atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt
     5041 cgatacttcc aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct
     5101 ttcctcgatg tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc
     5161 gatatgttct tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat
     5221 tttttcatct ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc
     5281 tatagctgac taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg
     5341 ctgattatat gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc
     5401 agattcgtaa tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc
     5461 gtattttaga aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact
     5521 cgatggattt ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt
     5581 acacagcgaa aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg
     5641 acttcaccta tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg
     5701 atgcagcaca aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg
     5761 atttggagat cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg
     5821 tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg
     5881 ccaaatcagg gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac
     5941 caagtggaga cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt
     6001 accgcggtgt tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa
     6061 tcaaactgat gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa
     6121 aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca
     6181 aaactgtcca agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg
     6241 aagctttacc aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg
     6301 atacttctcg ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg
     6361 ctttacaatt cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc
     6421 tcggacttct tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat
     6481 tgatcacgac tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta
     6541 cagtagataa cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat
     6601 tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc
     6661 aacgaaaacg gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg
     6721 tctataatat tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca
     6781 cgaaaggcta tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga
     6841 agatggacat cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag
     6901 actttgccta tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc
     6961 aacaatttga ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca
     7021 tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac
     7081 gtaaactctt agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg
     7141 aagttccgca agaagccttc atggccgttc tgaaaatgga cgaggacgaa ccgaagaaat
     7201 agcaagggtt tgtttgaaaa cagtgtaaaa aataaaccat gtttacatgg atttgccacc
     7261 ggtttgccac cggtggtttt tttcggtggc aaaaatcaag aattctttct aagattttcc
     7321 atgatatcga ccgtttcatt tctcatctta tctgttacat gcgagtaagt atccatcgta
     7381 attgaaattc tgctgtggcc caatcgttca gagatttctt tcatttttgc gccattttcg
     7441 agaagaagtg tagcatgagt atgtctgaga gaatggaagt taaaagagag ggagagtgca
     7501 ttcgatatcc tcctagtatt ccatttcact acacttggcg taactagctc accgtcttcc
     7561 ttcgtacata ctgcatttga atcaatgtag agctttccat acttcattcg attttctaat
     7621 tgttgtttct tatgtttttt cagaattgct agcaaggttt gtccaataaa aatcgttcga
     7681 ttggaactgc ttgtctttgg cgtaccatat acccatgcgc cgtcattctt taccatttgt
     7741 ttctctacag taattgttcc atttgaaaaa tcgacatcat cccacgtcag accacaaact
     7801 tcgccaacgc gcattcccgt ataaaatcca atattcaaag gaatatagaa aggatgtcct
     7861 tcaggagtaa tttctagcat atgatcaaag tcctcaagag aaatgatttt tagatctttt
     7921 ttagtcgttg gtcgtgcttc atattttggt atttttacat acagcatagg attttgcttg
     7981 attaatcccc aaggataaac cgccatattc agcgcattct taaggacaga gtgagtaata
     8041 gtcattgttt tcttcgagta acccttttta aattcagcat tgatgaaatt ttgtaaaaga
     8101 gcaggggaga gatccgtaag ttttttcttt cctaaataac cgtttatatg atttttgatg
     8161 gtaaatcggt agttttcata ggtattgtat tttagattta gtttaacgta ttcctccatc
     8221 caaaaatcaa ggtattgttt tactcgagta tccgtaccta aaaagtattg tcctgtttcg
     8281 tcaatatctg ataaaacttt tcgtaaagca gcttcggcct ctggtcgggt gtctccgcca
     8341 actttctcca ctttttttct tgagccatca tcattgatat ct
//
LOCUS       DAPCMU010000116         8330 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPCMU010000116 DAPCMU010000000
VERSION     DAPCMU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.119"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            138..689
                     /gene="lepB"
                     /locus_tag="RD744_002166"
     CDS             138..689
                     /gene="lepB"
                     /locus_tag="RD744_002166"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDU0558149.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(772..1272)
                     /locus_tag="RD744_002167"
     CDS             complement(772..1272)
                     /locus_tag="RD744_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDU0558150.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1551..1949)
                     /locus_tag="RD744_002168"
     CDS             complement(1551..1949)
                     /locus_tag="RD744_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0558151.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2209..3069
                     /gene="ylqF"
                     /locus_tag="RD744_002169"
     CDS             2209..3069
                     /gene="ylqF"
                     /locus_tag="RD744_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDU0558152.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            3062..3829
                     /locus_tag="RD744_002170"
     CDS             3062..3829
                     /locus_tag="RD744_002170"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDU0558153.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3885..4742
                     /gene="dprA"
                     /locus_tag="RD744_002171"
     CDS             3885..4742
                     /gene="dprA"
                     /locus_tag="RD744_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDU0558154.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4864..6942
                     /gene="topA"
                     /locus_tag="RD744_002172"
     CDS             4864..6942
                     /gene="topA"
                     /locus_tag="RD744_002172"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDU0558155.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6911..8287
                     /gene="trmFO"
                     /locus_tag="RD744_002173"
     CDS             6911..8287
                     /gene="trmFO"
                     /locus_tag="RD744_002173"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDU0558156.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2907 a         1457 c         1743 g         2223 t
ORIGIN      
        1 ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat ttttatttca
       61 gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg aaaaaaagag
      121 ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc tggcttgtat
      181 tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt ttattcatcc
      241 cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga
      301 tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta gcagatggca
      361 cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac caaaatgatc
      421 aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac ctgaaatcag
      481 atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg ggacaatcca
      541 agcttccgga agataattat tttgtacttg gagataatcg aagagtttca aaagatagtc
      601 gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt gtctattatc
      661 cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc tgttgagact
      721 gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa atcaaacagc
      781 tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa ccattatttg
      841 aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt tgaaatctgc
      901 accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga tcataatcaa
      961 taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa ataagccatt
     1021 aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa
     1081 tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga tcggtccata
     1141 tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc
     1201 aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg tcgacaacaa
     1261 tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt
     1321 ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg tcatgacaca
     1381 ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag actgctttta
     1441 taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat acttatcgta
     1501 tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc ttacatcttc
     1561 atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga aaacatttct
     1621 tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt tgcaatttga
     1681 gcaaattttt ttacttcatc cgcgttttca acactaaacg aaatcaaagc accacttgtt
     1741 tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc atgagagaga
     1801 agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt agaaaattct
     1861 tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac atcagcaaca
     1921 ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt ttgttcgtct
     1981 atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga aaccaaaata
     2041 aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt cactttcact
     2101 taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa aaagaaaaaa
     2161 acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat gaccatccaa
     2221 tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat gaaatatgtg
     2281 gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa tccaatgcta
     2341 gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga tctggcagat
     2401 ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct tgcattagct
     2461 atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa agaagcattg
     2521 aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat tcgggcaatg
     2581 gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt aggcaagaaa
     2641 attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct tcgtttaggt
     2701 aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga agatgaagaa
     2761 atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca tttagatgat
     2821 ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat cagcgatcgt
     2881 tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat ggatattaca
     2941 gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt gcatgatatt
     3001 cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga gcaaaaaagt
     3061 gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga gaatagatga
     3121 tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag cactggcttc
     3181 ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag aaatgttttg
     3241 ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg atgaagtagg
     3301 aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga aaaatgagat
     3361 catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac ttctttatca
     3421 acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc agacaatcga
     3481 tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag aaaaattgtc
     3541 cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta ttcagcagga
     3601 aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta tcattgctaa
     3661 agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat atggtttctc
     3721 gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac aaggaattac
     3781 gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa aaacagccgc
     3841 ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat caaatagaag
     3901 aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa tgggcacttg
     3961 ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc attaagatag
     4021 gtaaaataac caaacatcgg gagctgtttc gcaaaagttg gcaggaaatt cacgctaact
     4081 ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa tatccccctc
     4141 agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat ctatctttac
     4201 tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta gccaaaaaaa
     4261 cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc agtggctgcg
     4321 cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga cggacaatcg
     4381 cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac ttgcaagagg
     4441 tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc ccgaaaaagc
     4501 attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca tgtgtctttg
     4561 aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat cttggaagaa
     4621 cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt catcatttga
     4681 tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa tttaaagaat
     4741 aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat atgattagct
     4801 acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg agtccattca
     4861 gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa gacaattgag
     4921 aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag agatttaccg
     4981 aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat ttctatacgc
     5041 ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca aaaagtttac
     5101 ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc gtacctactt
     5161 gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa agacgcagtc
     5221 aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc acagcaagct
     5281 cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg gcgtaaggtc
     5341 aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat tattgaccgt
     5401 gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg taattttaaa
     5461 aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa aaagaaatta
     5521 ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga cttcgaagtt
     5581 aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac tacaagtagc
     5641 ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat gatggtagca
     5701 cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt gattacatac
     5761 atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc agcatttatt
     5821 gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa aaatgctcaa
     5881 ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac accagatgaa
     5941 ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg gtcacgtttt
     6001 gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt agaacaaaat
     6061 ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat gcaagtgtat
     6121 gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga acttgaagaa
     6181 ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca accaccagca
     6241 cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg gcgaccttcc
     6301 acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt gacacaaaaa
     6361 agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga attcttccct
     6421 caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa aattgaagaa
     6481 ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga aaaagaactg
     6541 acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc aggattcgat
     6601 tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa gttttatgct
     6661 tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat cggtgtgact
     6721 tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa cagactattc
     6781 tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc agtgggcaga
     6841 aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg taaacaagta
     6901 gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag aggttctttc
     6961 attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg cttggcagat
     7021 cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat cgactgaggc
     7081 gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag gaaacagttt
     7141 ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg tagtaatcac
     7201 ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc gcgattcttt
     7261 ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga taaacgaaga
     7321 aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga cttctcatcc
     7381 attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct atgatgcagc
     7441 tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga agtcccgcta
     7501 tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat tcaaagcttt
     7561 ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga aagaaaaata
     7621 ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa cgatgctttt
     7681 cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc cttatgctgt
     7741 tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg gctttcagac
     7801 tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt tagaaaatgc
     7861 tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc cagaattgct
     7921 gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc agatgactgg
     7981 tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca acgccgcacg
     8041 cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg gaagtatggc
     8101 ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca actatggtct
     8161 gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag caattgcaca
     8221 acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg caatcccaaa
     8281 tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
//
LOCUS       DAPCMU010000117         8260 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPCMU010000117 DAPCMU010000000
VERSION     DAPCMU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.120"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..416
                     /locus_tag="RD744_002174"
     CDS             <1..416
                     /locus_tag="RD744_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDU0558157.1"
                     /translation="LKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGII
                     LDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQG
                     LGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            431..1183
                     /locus_tag="RD744_002175"
     CDS             431..1183
                     /locus_tag="RD744_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0558158.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1886..2101)
                     /locus_tag="RD744_002176"
     CDS             complement(1886..2101)
                     /locus_tag="RD744_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDU0558159.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2120..2356)
                     /locus_tag="RD744_002177"
     CDS             complement(2120..2356)
                     /locus_tag="RD744_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDU0558160.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2614..4767
                     /locus_tag="RD744_002178"
     CDS             2614..4767
                     /locus_tag="RD744_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDU0558161.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4736..6151
                     /locus_tag="RD744_002179"
     CDS             4736..6151
                     /locus_tag="RD744_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDU0558162.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6184..7218
                     /gene="sppA"
                     /locus_tag="RD744_002180"
     CDS             6184..7218
                     /gene="sppA"
                     /locus_tag="RD744_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDU0558163.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7220..7741
                     /locus_tag="RD744_002181"
     CDS             7220..7741
                     /locus_tag="RD744_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDU0558164.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7738..8124
                     /locus_tag="RD744_002182"
     CDS             7738..8124
                     /locus_tag="RD744_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDU0558165.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2938 a         1191 c         1486 g         2645 t
ORIGIN      
        1 atttgaaaag tttgttgata gcgaagctat accaagcaaa taacaataag atttgttgta
       61 aattcgaatg tccaagtact attattgaag ttcctgttcc aatatttgac tgtattaggg
      121 tgcttggaat tattctcgat aatgcaattg aagcatctat agagagtcaa gatagagaac
      181 tgtctctagc tatttaccga gatgataaac agatagaaat ttcgatatcg aatagttgta
      241 gagagatact actctctata gatactctaa tgaaaaaagg aataacaaca aaaaagggac
      301 atcaagggtt aggtttatct agtatagagg aaatcaataa aaagaatccc aatatgtttg
      361 tgaactatca aaacgatccg tatcaattta ctactcaagt tattttgttg ttttgaggga
      421 aggaggattt atgagttatc caataatatt atgtgaagat caaattatac aattaaatca
      481 gttagaacga attattgata atttcattct ttttcacgat aaagtattta agatagtatt
      541 gaaaacacaa agtccgttag aagttaaaaa atacctcaaa caattccgac caaagcaagg
      601 aatatatttc ttagatattg atttaaatca tgaagttaac ggtatagaat tagcagaagt
      661 aatcagaaaa tatgatgttc aagcaaaaat catttttaca actactcatg atgagatgtt
      721 acccgtaaca ataaaaagaa gagttgaaac gttaggattt gtaacaaaag atcaaacact
      781 agatgagtat cgaaacgaga ttgttgagtt attgttatta gcgcaagaaa ggatagatgc
      841 aacaaaacag gttacgaatc aagcatttgt tttttccata ggttctcaaa catttacttt
      901 tgatataaat gacatttatt ttgtagaagc ttcaaaatta ccacatagat tatcattgtg
      961 tactaaagat gggcagtacg aattttatgg aagaattagt gagcttgaaa agaaatatcc
     1021 tatgttaaca agaattagtc gagcgtgttt agtaaatatc tttaacgtta aagaaataga
     1081 ttttaaaaaa cgttcactat attttgattc agagttggcc agaaacttta ctttagggaa
     1141 agcacaaaaa attaaggaga agttgaaaga aagcactgtt tagtggtttt ttctttaggg
     1201 agattgcatt tttctaataa aatagattcc ttcactagtg caaatttttc tgaatcctat
     1261 taaagttgta ccaattttta tagctgttat tagttacgtg cataacagca atattagtta
     1321 ctggaggaat atttctatgg tctggaacca aaaaagggcc aaatgaatag ttcttttctt
     1381 gtttttatta agtaccataa aggggaatta aaattaaatg tatggtggaa gatgcgaaag
     1441 atttggcaaa ttctactaat ttttagaaag tgtttttcat tttttagtta aaaaatttgt
     1501 cctgtcaagt gcaatcctat gattgtactt gataggattt gagataatta ctttcctgtt
     1561 tttataaatt taacttgcag gaacaggctt taatgcacat ccgacaagta cattaccaat
     1621 acctcctgta acggccgaaa taggtcctcc tgcagcacca actacagcgc tgcaagtcct
     1681 tttgtaaaaa tatctctacc attacatttc caaagaattt ttatctgact ttaataaaag
     1741 ataaaaattt aatataatta taaaaactag cacaagaagc tttagtaaaa agttctgtcc
     1801 attccaaact tttgaaaaga taaagaacat acctatgttc gtaatcgaca caaaccaatc
     1861 ttgaggtttg atatttttta attgtttacg caccggtcca catgctgaat ccagcaccta
     1921 ataaagctcc acctacacca caagcagcta ttgcataagg tcctccggct gaacaaagtt
     1981 ttactcctgc tgttgcccca gaaaaagtag tgcctaaaac tcccacggca ctcccacctt
     2041 gaatattttt caactcttct gaagataata taaatgttgt ttttgtgttt ttaatatgca
     2101 tactacttat tctccttttt tatttttttc gagcatccca gccagctgca gcacctccag
     2161 cgacagctcc atataaccct ccaacaacac cgcaagctgg tccgcaaatt gctgttccta
     2221 aacctgcacc acctactgcc cctgtggtaa gtccacctac aacttgcccc aattttcccc
     2281 aagcattatt gcctccacca acagtttgtg catttccaca atacttagtt cctttacatt
     2341 ttgcatgttt aaacacgctt ctttaaataa tattttttac ggtgctactg taacatacgt
     2401 atttgattaa cagtaaaaat cttcctgaaa cttatcaagt ttattcttga atagtaaaat
     2461 ttccaaaata aatttgattc aggaatgaaa aagttagtgt tcaagaactg ttttcaaagt
     2521 ttttgtggaa ttatgagaca atattttcac tgtattaaag ctatattgag aatttttcta
     2581 taaaaatttt ttagaaggag ttgtgttaga taagtgataa aatcatttcc ttttgtgcag
     2641 caacaagatg aaaaagattg tggtgttgct tgcctttcga tgattttaaa atattttaaa
     2701 actgatatcc cgattcataa attacgggac ctatctggga ctgatttaga gggtacttca
     2761 gcatttggat tgaaaaagac atttgaaaaa ttgtcttttg attgtatggc tattcaagca
     2821 gatagtgaga tctggaaaga aagagatctt atcttaccat taattgcaca tatattggtt
     2881 gataacagct atatgcacta cgttgtaatt tataaaacga agggaaacgt actatggatt
     2941 gcagatcccg ctaatggaaa aaatagaaaa acgattgaag cgtttcaaaa agaatggaca
     3001 ggtattcttt tattacctat gcctagagaa acttatattc ctattaaaga aaaagtagct
     3061 ggattaagtt cttttttgcc tataatttgg aaacaaaaag gattagtatt ccacatcgtt
     3121 ttagcatcga ttttcattac gttttttggc ataggaagct catattattt tcaaggtatt
     3181 cttgattttt tcatccctaa tcaagcacgt tcaactttaa atataatttc cattggcttg
     3241 atcgctgtct atttatttcg ggtattgttt gaatatagtc gtagttatct tcttattatt
     3301 ttaggacagc gaatgagtat ggcaattatg cttcagtatt tcaaacatgt gttaacttta
     3361 ccaatgaatt tttttgctac gagaaaatca ggggaaatta tttctcgttt tcttgatgcc
     3421 agtaagatta ttgatgcttt agcaagtgct acgttatcaa tatttttaga tattggtatg
     3481 gtgttgcttg ttggggttac gttagctatt cagaatagta cgttgttttt aatcactcta
     3541 tgctctttgc ctttttatgt agttgcaata ttcgctttcg tgaaaagcta tgaaaaatcg
     3601 aacagggaag aaatggcagc cggtgcagct gtaaattcta gtatcataga aagtcttaaa
     3661 ggaatagaaa caattaaagc atatagcggt gaagagaaag tatataaccg tatagaccat
     3721 gaatttgtta aattaatgaa aaaatcattt cgtacggtta cgcttgataa tattcaacaa
     3781 ggaattaaac atttgattca attaattagc agtgcattaa ttttatggct aggttcttat
     3841 tttgttatgg ataatagaat tagtctaggt caacttatca catacaatgc tttactagta
     3901 tttttgacag atcctttaca aaacattatt aatttacaag tgaaaatgca aacggctcaa
     3961 gttgcaaata atcgtttaaa tgaaattttc gcgattgatt cagaaaaatc gaaccagtct
     4021 tttgaatata tgctttctga acaattattt caacaagata tcactgtaaa aaacgtttca
     4081 ttctcgtata gcatgaaggc cccaacgttg aacgaaattt catgtacaat tccttcgcac
     4141 agtaaaacgg ctatagttgg agttagtggt tctggaaaat caactcttgc aaagttattt
     4201 gttcgttttt atgaagcatc agaaggatcg atccattatg gaaacattaa tcacttagac
     4261 attccttttc agatattacg cgatcgagtg acatatttgc ctcaagagtc atttttcttt
     4321 agtggaacaa tcattgagaa tcttatattc ggtttagata gcccaccaag tttcgaacaa
     4381 ataatagaag tttgtgagat agtccaacta aatgaattta ttaatcaaca gccattacgt
     4441 tacgagacat tgttggaaga aggtggaaat aatctttcag gagggcaacg acaacgatta
     4501 gccttagcac gggctttatt aaaaaatccg gacatattaa ttttagatga agcaactagt
     4561 ggattagata ccttgttaga acatgcaatt atgaattatc tcctcaattt aaaagaaaaa
     4621 acgattattt ttattgcgca tcatttacca atagcaaaag catgtgatca aattttagtt
     4681 ctccatgaag ggcatttggt agaaaaagga acgcacgatg aacttcgttt tgccgatggt
     4741 atctatcaaa aattatggga aatctaggag agggaaacaa tttatgcaga ataataattg
     4801 gttcgaacat tctagtatct atactaaaca acatcatagc ttctaccgtt gggtgttata
     4861 ccctgtaatt ctttttttat gtattattgg attgttttta acctttgcta aaaaagaggt
     4921 tgttattcgg acaacggctc aattgaaccc cgaaaaaata gagaagttac aagtgccatt
     4981 agaagctaaa ataatggaaa acttcctaaa agaaaatcaa tttgttcata aggacgatgt
     5041 gttggttaga ttggattgtt ctttaataga gaatgaaaag gctcagatag aacaagaaaa
     5101 ccaacgtatt actcaacaaa taaagatggc tcagctattt attgaaagta taagtaaagg
     5161 aaaaaatttg ttttcaacgg atgacagttt tggctacagt aatcaattaa agagcatgtt
     5221 gtcagaaaaa gaatcactcc gctacgcttt gaagcaaagt gaattaaatg atcaaaagca
     5281 attagaagta tacgaaaaga caaaaagaca actagaaaaa caaattgaga gttcagatag
     5341 taaattacaa gaatggcaac aagtacaggt agcttggagt aataatcaat cattaaaaga
     5401 tttttcaaaa gaaatgatgg caaactatga gaattggcaa gaacaactaa ataatgtttc
     5461 tgatgatcaa aaaaatcaag tgaaactgac aatttcagca agcataaatg aacaaattga
     5521 gcaactaaaa aaagaagtag aacagtatca gtcagaaaaa gctaaattag ttaaaccaac
     5581 tacttctgag aatgacagaa ttagtcaaac ggaaaaagga aagcaagagc tagaacaagc
     5641 aataacaact gtaaaacaaa cagttacaga gctaaatgaa aaacaagaga agaaccagtc
     5701 agttatcaaa agtttggacg atcaactttc aaaaggaata ctaaaagctc ctgtatctgg
     5761 aacggttcat ttaaatgaag aaacaaaggg acaagcagaa ctagccaaag gaacagtgct
     5821 tgcagaaatt tatccgatcc acgaaaaatc aaaaatgaaa tttacagcac ttttacctgc
     5881 gaatgaaagt acctatatta aagaagggat gaaggttcat tttaaattgg atcaaaaagg
     5941 tggggctccg attacgatcg acggaacttt agatgaaatt tcagaaaatt ctactgccac
     6001 tgagaaaggt gttttttatg cagtgaaagg aacgttaaaa acaactcaat cgtttccgtt
     6061 tcgttatgga ttaactggag agttgtcttt aattgtagga gaaaaaacat attggcaaca
     6121 aattgtcgat gtgtttctaa ataaaaaata attattttta ctaagaatat aggaggagtt
     6181 tatatgaata aaagaagatg gattgctgtg ggagtagcgt gcggattatt gttactatcc
     6241 attatcgtcg cgctaattcc aggaaaagat aaagaggaag catcaaatac aaccctaaca
     6301 ggaataaata agatttttta cggatctaac gaaatcacag aagaaacgtt agaagaaggc
     6361 gcatcaaata aaaagattgt caaactatcc gtcaatggag ttattgcaga tacaggtgaa
     6421 agcaatcttt ttagtaggga acaatataac catcagaatt ttttaacaca gctaaagaaa
     6481 attcaagaag acaaagcggt taagggagtg ttattagaag taaattcacc tgggggagga
     6541 atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct agatattcct
     6601 atctatactg catttaaaaa tacagcggca agtggagggt actacatttc tgctggttca
     6661 gataaaatat ttgctacgga agaaacaaca actggatcaa ttggtgtcat tatttctggt
     6721 ttaaattatt ctggcttatt agaaaaactt ggcgtaactg atgcgacgta taaaagtggt
     6781 gcattaaaag atatgatgtc acctcaacac aaaccttcag aggaagagaa taaagttata
     6841 caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa aggaagaaat
     6901 atggatacta atgcagtgaa agaacttgcg gatggaagaa tttatgatgg taaccaagca
     6961 gtagaaaatg ggttggtgga tcaaattggt tactctgaag atgcattaga ttccctaaaa
     7021 aaagagaaga aattaactga tgcgacggtt atagaatata aaaatgacac aactggcttt
     7081 gcttcttctt ggttaggtaa taagattgct gaatggcaag ggctaaaggc aactacatca
     7141 gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg atactccaaa accaatgtat
     7201 tactatggag ggctataaaa tgaataagaa gagtggcatt gaaactttgc ctaattatgt
     7261 atttgcggga ttttggttac gattttttgc ttttcttatt gatttaattt gtattagcat
     7321 catcacccat gctactatag gtctctttta caaaatcatg ggactggaat ctgacaaatc
     7381 cattattact ttttatggta tagcgtcatt agctatttat ttaacctatt ttgtactttt
     7441 gactaagctg aattcaggac aaacaattgg gaaaatgatt tttgggataa gagtagtgag
     7501 ctttgaggaa aaagagctaa gttggcaaac tatctttatt cgtgagtgta tatgtaggtt
     7561 tatattaaaa aattttgtat ttcaaattgg gtatttggtg gctgctttca cgcccaagaa
     7621 acaacatatt ggggattatt tttctaacac gtcagtggtt gttattcgtt cattagaagc
     7681 tattaactat ggtcaaaaaa tttctaattc taaaacactt aatctggaga gaattaaatg
     7741 aataagagaa aaacagagtt tattttgatt ttaattagtg gaataacagg gattttatgt
     7801 agtcttttag cattgatggg tggtatgttt ctatcttcta ttgattcaac tactttaaat
     7861 actacagaac taaccagtaa ggatatagat atgttagtca atctaagcgg gaatgttgtt
     7921 ttatttggag gaattaccct agttttttcg ataatgattt tcataatagg ttttttagta
     7981 agaaaaaatc aaaaaacagt ggtctatggt attagtttac tcattttatc tattattcca
     8041 ttttttcttt taacattatt gtgggtaatt cctggagttt taggtattat tgcgggattg
     8101 atgcttctaa taagaaaatc ttaattaggc aatgtgtaga caacgtgaaa cgattaatca
     8161 attaaaagct aaatacgttt tgagaaatga taacttgtag tcgatggaag acatgtagat
     8221 aaaaaaacta cgtgtctttt atttattcta attttttcga
//
LOCUS       DAPCMU010000118         8150 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPCMU010000118 DAPCMU010000000
VERSION     DAPCMU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.121"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            117..581
                     /locus_tag="RD744_002183"
     CDS             117..581
                     /locus_tag="RD744_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0558166.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            595..1344
                     /locus_tag="RD744_002184"
     CDS             595..1344
                     /locus_tag="RD744_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0558167.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1344..2174
                     /locus_tag="RD744_002185"
     CDS             1344..2174
                     /locus_tag="RD744_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0558168.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2174..2821
                     /locus_tag="RD744_002186"
     CDS             2174..2821
                     /locus_tag="RD744_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0558169.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(2919..3695)
                     /locus_tag="RD744_002187"
     CDS             complement(2919..3695)
                     /locus_tag="RD744_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0558170.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            3839..4798
                     /locus_tag="RD744_002188"
     CDS             3839..4798
                     /locus_tag="RD744_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558171.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4915..5223
                     /locus_tag="RD744_002189"
     CDS             4915..5223
                     /locus_tag="RD744_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDU0558172.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            5250..5549
                     /locus_tag="RD744_002190"
     CDS             5250..5549
                     /locus_tag="RD744_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDU0558173.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            5723..6274
                     /locus_tag="RD744_002191"
     CDS             5723..6274
                     /locus_tag="RD744_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDU0558174.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            6420..6713
                     /locus_tag="RD744_002192"
     CDS             6420..6713
                     /locus_tag="RD744_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558175.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            6706..7002
                     /locus_tag="RD744_002193"
     CDS             6706..7002
                     /locus_tag="RD744_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558176.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            7073..8077
                     /locus_tag="RD744_002194"
     CDS             7073..8077
                     /locus_tag="RD744_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDU0558177.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         2877 a         1184 c         1523 g         2566 t
ORIGIN      
        1 ggaattactg aataactctc aaaaaagtat ggttcatatt aattcgcttt taaaagatgt
       61 cagttaaggt ggcaatttct attctataaa ttttaaaaat tgaaggagga aatagaatga
      121 ttaaattagt gcgtgtagac cacagattac ttcatggaca agtaatattt tcttggacta
      181 aacagtatga tattaaccat attattgtgg ctgataacaa agttccagga gatccaatgt
      241 ctgtgatggc tttgacaatt gctaaacctg ctgattgcaa attagacatt gttaaaatta
      301 gtgatgttaa agggattatg gaaacatccc caaatgataa ttacatgatt ttaattaaag
      361 gacctaagga agcttttgaa ttagttcaaa atttaccaga aattaaagaa attaattttg
      421 gcggtgtgtc taagaaaagc gagtccaaac aatatggaaa ggcagtcttt ttaactcctg
      481 aagaattaga agccacaaaa aaaattcttg atcaagaagt gaaaatattt gtgcaacaag
      541 tcccatcttc aagtattgaa aatgtagatt ttagtaagta aggggggaaa ataaatgtta
      601 ttaaaagcca ttttattagg tttagttggt gtggtagctg ttattgactc caggttaatt
      661 ggtcgacaaa atattggtag accactaatt ttgagtacac ttgccggact tgtgctaggt
      721 gacttgcata cagggattgt tcttggggca tctttagaat taatttctat gggattcgtc
      781 tcaattgggg cggctggacc tcctaatatg caacttggta gtattattgc cactgctttt
      841 gcaattttaa ctaatagtag tacagaaaca gccttaacaa tagctatccc agtggctgta
      901 gctggtgaat ttatgagtat tatcatgcgc atgataattg ctcaatttgg acatgctgca
      961 gataaagcca ttgaaaataa taaatttaga aaagctcaaa ttattcatat ttattggtca
     1021 tttattttta atgcttttgt ttactttatt ccaatctttt taacagtata ttttggtgct
     1081 gacgttgtgg cagatttagt agataaaatt ccagaaatga ttacagacgc cttgactgtc
     1141 tctggaaata tgctttccgc attagggttt gcaatgctat taagcacaat gttgagtaaa
     1201 aaattatatc cttactttat ttttggattc tttattgttg cctattcagg tctttcacta
     1261 attggcgtga caatttttgc agctctgatt gcctttgtaa tggatcaagt taaatatgga
     1321 aaaagagaag aggcgcatgg ttaatggaaa atagtacaga aaaaaaacgt ttaatcacaa
     1381 aaaaagatct taataagatg tgtcttcgtt catggacaat catgtgttcg tggaattatg
     1441 aaagacaaat gcatatggga tttatgtatg ggatggcgcc aattttaaat aaagtttata
     1501 aaaatgatga agagaaaaaa agagaagctt atactcgtca tatggcattt tttaactgta
     1561 cgcctcaaat gacttcattt attatgggac tttgtgcttc tatggaagaa caaaatgctc
     1621 aagcagaaga aggaaacttt aatactgaat ctatctccat gattaaaacc agtttaatgg
     1681 gtccttttgc tggaattggt gattcatttt tccaaggtac tttaagaatt attacatttg
     1741 ggattggatt gtcttttgct aagcaaggta gtattttagg accgatttta gctgttggcc
     1801 tttttgctat accttctatt ttagctgttt tttacggcgg taaattagga tatgagtctg
     1861 gcaataaata ccttactaaa ttgtatcaag aaggtcttat ggatcgagta atgcaatttg
     1921 cttctatcat tggtttggct gtagtaggtg gaatggtggc tagcatggtg ccaattgtca
     1981 cccctttaaa attcactaca gggggaacag aacttgtttt acaagactta cttgattcca
     2041 ttattcctgg attattacca cttgtatgta ctttgggaat atataaattg attaagaaaa
     2101 atgttagtac aaatatgctc ttattaggtt gtattttggc agggtttgtt ttaagtataa
     2161 taggtatctt ataatgatta aggctctaat ttttgatatg gacggagttt taattgatag
     2221 cgagattatg tatcaaaagt gggtatttaa ttttttaaaa caagaaaatt taagctatcc
     2281 tttgtattct tatcaaaaaa agattggaac tacagcaagt atttttgatg atatagaaaa
     2341 gtacaatgag ggatgtaaca gtttagaatt gaagggaaaa tttactaaat attggaaatc
     2401 gcaaaagatt gattacttgg atatttttcc taatatacta attgaacagc ttgaatggtt
     2461 aaaaaagaaa aaaataaaaa tggcaattgc ttcttccagt ccaatggata ctataaatga
     2521 agttgttacc acttgtaaaa tagagtcata ttttgattgt ttaattagcg gaagagattt
     2581 gccagaaagt aaacctaatc ctactatatt ccttaaagct tcagaacagc tcagaattcc
     2641 agttgaagaa tgtctggtga tagaagattc ttataatggt ataaaagctg gtaaaagagc
     2701 aaatatgaag gttcttgcaa ttaaagataa acgtttttct caagatgtaa gtctagcaga
     2761 tgatgttatt tacgatatta aatatctgcg acaagaagta caggtgaaat ttggaatata
     2821 aataagtttt ttgatgtatt tagagaatat tagtaaaaat aaaaatcatg cccaaatcta
     2881 atcaaaattg attgctttgg gtatgatttc ttttaatatc aatctattac tttaggatct
     2941 gaatcatttt tatgcaccgt atctaaagtg atattttctg tgacttcaat aacataacta
     3001 attctatcac aacgaactgt ggattgagaa taatgaaatg gttgattatt tttatctttg
     3061 ccaacctttt ctaaaataaa tagcggatct cctactatac attgcaataa gtcggaaagc
     3121 tcaggtgttg cagataaacc gtttatttcc ataatagagc tcgtagcttt gacatcatag
     3181 tcatcttcta gaagtttata taatgaagta tattggctac ctttttcaaa taaagttgga
     3241 aatttttctt ctgatgcgaa aaaattatca attcccatcg gggcaccgtc ttcccacatt
     3301 aaacgtttaa tattgtaaac tctatcagtt acattgattt gtaatttatt agctaaaaac
     3361 tcatcagcct ttataatttt agaatgtaaa acagtatatc taattttatg tcctcgggta
     3421 gaaagagaat cgctaaatcc atgaactcca gctaatttta aactttcttt tttttgagtt
     3481 acaaaggtcc ctttccccct taatcgttca actgttccac tttctgctaa aagtttaaaa
     3541 gcttttcgaa tggtaatctt acttactcca taaattttac ataattcttc ttctgtaggt
     3601 aattgctctc ctggaagata ttttttttca cgaatatcgt tacgtataga agcttctact
     3661 tgtaaatata atggtctatt atcttgaaaa ttcatagtac actccctaaa aagttatact
     3721 tcattatatc acaatttatt aaaacgtcga ttgtaaaatt aagctagaca actaaaaaga
     3781 tcatttgtgc tacactcaaa atagttccga ccaaagaatt aataaggagt gagtacaaat
     3841 gacttacacc catcttacaa cagacgaact agtaatcata gagtcttatt tcaatatcga
     3901 gaaacctgtc gctcttgtcg cacaaagttt aaatcgctct agacaaacca tttataatgt
     3961 ttattcgttt ctcaaacaag gaaaaaaagt gctagattat taccgtcaat acaaacaaaa
     4021 taaatctcgt tgtggacgac gttcaaccgt tttatctgat gaacaatcaa catatgttca
     4081 agaaaaggta gctcaaggat ggtcacccga tgtgattatc ggccgtggcg agtttccaat
     4141 tgattgttct gttcgtacac tctatcggat gtttaaaaaa ggcctttttg acacacagtc
     4201 gttacctatg aaggggaaac gtaaaccgaa taatcatcaa gaaaagcgtg gaaaacaggc
     4261 ctttcgcaga acgattcatg aacggaacag tctctatcca cacctgacta aagaatttgg
     4321 tcatctcgaa gtcgacacca ttgtgggagt taaacacaag agcgctgtta ttacattggt
     4381 tgagtgttta tccaaggtta ttattacttt gaaaccgaca ggcagacgag ccgaagatat
     4441 cgaaaacagg ttaaatgatt ggttcaaatc catgcctaaa cacttgttta aatccatgac
     4501 atttgattgt ggaaaagaat tttccaactg gcgatctata agtaactcga acgatattgc
     4561 catctttttc gcagatccag gaacaccttc gcaaagaggg ctaaatgagc actcaaacgg
     4621 cttgcttaga cgagatggct tacctaaaac aatggacttt agggacacgg atgaaacctt
     4681 tattcaatcc gttgcctcga aaagaaacca tattccaaga aaatcattaa attatcgaac
     4741 accattggaa gtatttttga gctgcataga taaagacata ttgtctagct taatttgaca
     4801 aatcagatag tataaaatga atcaaattga atatcaaata tatattatat ccatgaagcc
     4861 caatcttctt taatagaaga ttgggctatt tttatggcaa aggagggaaa agaaatgttt
     4921 caaaataaag gacaacgtta tgtcacaaag ggagtaatgg acagtttgcc ggtggaatta
     4981 caagctttat gttggaatct aatcgatcaa aacgttcaaa agcaatttca tttagactat
     5041 cttcaaatct ttgaattttc tacggaaaaa ggaaatcaaa agttggttca tcgtcaagaa
     5101 gaaccagaag caagaaaaga gtatctgatt tcacctaagc ttcgtttgaa atcagtgtct
     5161 caaaaaatat gggtcattga ttcgggtgag tatcaaacga tgttattacc agaagaatac
     5221 tgatatctaa aagaaaggaa cgaaaggact tgttagaaac ggctatcgaa gtactggaaa
     5281 agtgtgcaca gctagtcact actcctgaag agtggggcta tgaatccgtt acgatggaaa
     5341 aagaggaaat tgaaatgggt atgcttcctg aagatgttac tctaccacgt ttagtgatga
     5401 cacatcttta tatttattgc gcaccagaag atggaaaaga ctatgtagtt tatttcatta
     5461 cagatattac cagccagcga gaatttgttc gtgggttact ggtagaagga cgattggttt
     5521 ggagtcagat tggaggaacg aatgaataag atcgattatt tgagaatgat tgcgaaatgt
     5581 ttagaagata tccttacagc gattcatcaa attctgaaga aaaaaggaga aagaatatga
     5641 aatattttca actagtattg acacttataa cggcaatggt gattgaattg cttgaacaaa
     5701 aattagcata ggagaaaaag agatgattga aaaagaatta gtgaccacac aaacagaagt
     5761 ttggcgagat cctcatcata cccatcgcta tgttttcaaa cgacaatggg cgatcaaagg
     5821 aaaagaagcg gaagataaat tagcggtagt cattactatt cgtccaacag atacagaacc
     5881 atttacgaat gatttgagta tgttattaat cgaaaagaat atccgacaat taggatttac
     5941 tggttttatt gctgtgaatc ttttttcgta tacaaaagcg aaaagtcctc gggtatttcc
     6001 tcgtggaaat gatgaacaat ctttggaaat attaacaact gttttcacag aaaagaaaat
     6061 caacaccatt atttttgctt gtggttcgat tacagcgacg agtcaagtgg cttatgaaca
     6121 agcgaagacc atttacgatc aattgagtgc gaaacaaaaa aagatgacca aagtattgat
     6181 aaatgcagaa ggtaagttat cccacccact aagtattcat gttcgtaatg aatggcaatt
     6241 ggcagatagt caattattat ttcaagaaaa ataacatttt attttcttat gttacttata
     6301 agtagtttta catgcttttt gactttaaaa atacatgtga tgatacatgt tatatgaatt
     6361 atcaatgtac ccagtaatat gctattttca ttatgtaaaa aataacaggg ggtgtgtaga
     6421 tggagattga tgaaacttgt ttgagtaact cttataaaag tatgaatgac ttttttgagg
     6481 aaatggaact ggattcagaa ttgctctatc aattttatct tgcttttcga gggcaacaga
     6541 tttcttttcc aatgaaaatg tacgatcgtg agttagttag aaaaagaatc gaaaacatga
     6601 tagaacagga aaaaactgta gatatccgtc aattgacaga agtttatggt ttctctgcaa
     6661 gatggattca aaaagtaatt aaacaaaaag aaaggagacg agtacatggc taagctgatt
     6721 gtattaggtg gtttaattta cggtggatat tggttatata aatatatgct tgcgcatccg
     6781 aatatagata aaggaaaagt aattaagaca gttggggtaa tcatagttgg actcatagct
     6841 ctaactaaga aaaataatac taatagccca aaaataatga gaacttatcg ctgtagagga
     6901 tgtggaaggg aaatttctac tagttttaat ccatctgaaa gccctcaagg aagagatcgt
     6961 tgtcatgctg gctatcgaca ttcttgggaa aggattaact aaatgaacac cattgagatt
     7021 aattgactga taaaataagt acgcgaggga gaaattgaaa gggggaaata aaatgaaaaa
     7081 atggttgtta ttactaattg gaatggggat tttattcact ggttgtacat ctgagaatac
     7141 gaggactaaa gagactgtag aaactgatac aacaattgag gctaaaaaaa gtaaaacgca
     7201 atcaactgaa caaactacaa cagaaatgaa acaatctgtg gaagcaagta caagtgaaag
     7261 ttcaatcatt gaatcttctt ctgaagaggt taaaaatact ttgtgggata gtgagaaatc
     7321 tactcaacta gcaatttttg tcccacaatg gggaaagaca ttaggtcaag aatataaaag
     7381 ctacaatcct cagaacaatg taagtctgta tggtacgcct ttaccaagtg cagttattga
     7441 tggaaattgg aaaatggcaa tcaatgaagc accagttacc gtacaatggt cagaagatgg
     7501 aactggtcat gcagatttca atttagtagc agtttattct gatgtagaaa ctggagaata
     7561 tttagggcag catgtatatt tctttggctt tcaaaatgga caacctaaag tttatttaac
     7621 gcaacaaaat caaggcaatg ataaaaacta catgtacttc aatgaaactc agaaccaaca
     7681 gttaaaacaa ggtttcattg atattgtgaa tggacaaact cctcaaactc cagtagtaga
     7741 agaatctact caacaagcaa caactacaac gacacaatct acaagtagct cagcaatttc
     7801 aagttgggaa gctgctaaag aaactgtact aaataataaa gctttatggt tgtatgattc
     7861 tcctgataaa aatgcatcaa atactaattt aattagcaca aatgaatata tgaaagatct
     7921 aatgcaagat gaaaatggaa gctactattc aattaatatt gtaacggatg ataatggaaa
     7981 tacaacaatg gctggtggaa aacaattccg tgtttataca gatggaagaa tttctcaacg
     8041 tattggaatg actaacaatt ttcaaatgat taaataattg aaaatattat ttatcaaaga
     8101 aaaactgatt ttgagaaaaa ttatttaatt ttcttagaat cagttttttt
//
LOCUS       DAPCMU010000119         8137 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPCMU010000119 DAPCMU010000000
VERSION     DAPCMU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.122"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(39..560)
                     /locus_tag="RD744_002195"
     CDS             complement(39..560)
                     /locus_tag="RD744_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDU0558178.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            759..4451
                     /gene="nifJ"
                     /locus_tag="RD744_002196"
     CDS             759..4451
                     /gene="nifJ"
                     /locus_tag="RD744_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDU0558179.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4640..5527
                     /locus_tag="RD744_002197"
     CDS             4640..5527
                     /locus_tag="RD744_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDU0558180.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5524..6693
                     /locus_tag="RD744_002198"
     CDS             5524..6693
                     /locus_tag="RD744_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558181.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6717..8072
                     /locus_tag="RD744_002199"
     CDS             6717..8072
                     /locus_tag="RD744_002199"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDU0558182.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2611 a         1504 c         1843 g         2179 t
ORIGIN      
        1 aaagtgttct ttacttttgt cccacgctcc ttattttttt attttgtttg gatcaattct
       61 tttaattcct ctattgaaat agattcatca aagatttctt tacccaagat gacagttgga
      121 acaaatcgaa tatgtgcttg ctcagcttcg ttgatgacag cttggctcag ctgttcgtct
      181 ttttgttctg tcagctttag tttatctact gcaaaatccg ctacttcttg gagagaaaga
      241 tgtttccact cgtcttgagt atcgaatatt tgtttgatct ctttgatcgc tttttggcca
      301 tcgctgattg tgagataccg gtgcatcacg ttccctcgct gtaagctttc tttttcttta
      361 tcgaacaatt tgatcactcg ctgcaattgc ccgctttgga ctgcttcttc taatagctca
      421 taagattctt caaaccattg tcggcaatac ggacaagcta agttgatgaa ttcgaccatt
      481 tttttaggag cattcgatga accataaagg attccttttt gtgtatttgt tttcgttgca
      541 tcaatcactg atatatccat gttaaaacac ctctttcgct tatagtttat acatgtctga
      601 caaaaaaagg caacgattta gactgtttgt ctaaaaatta gatgaccaat tcaaatagat
      661 acatgtaaaa tatttttata aataaaagaa aaaaatggca tgttttacgt tataatggaa
      721 cagataataa gagagaatat gaacaaggat gatgaatcat gcgaaaaatg aaaacaatgg
      781 atggcaatac ggcagccgca tatatttctt atgcatttac cgagattgct gcaatatatc
      841 caataacacc cagttcgacc atggctgaat tagtagatca atggtcagca gcaggaaaag
      901 aaaacgtatt cggtcaacca gtaaaagtag tagaaatgca atccgaagca ggagcagctg
      961 gtgtggtcca tggatcattg aaaactggtg cactgacaac tacatatacc gcttcacaag
     1021 ggctgctttt gatgataccg aatatgtaca agattgctgg ggaactactg ccaagtgttt
     1081 tccacgttgc tagtcgggca gtgacgacaa atgcgttgaa tattttcgga gatcacggag
     1141 acgtgatggc tgcaagacaa acaggatttg ctatgttagc tgaaagcagt gtacaagaag
     1201 tgatggattt agcacctgtt gcccatcttg cttctattga aggagaagtc ccttttttga
     1261 atttctttga tggattccgt acgagtcacg agattcaaaa aatcgaagtt ttggaatatg
     1321 aagaattagc tcctctgatc aatcaggaga aattagcttc atttcgtaaa cgagggatga
     1381 atcccaatca tccgactgtt agtggtacga accagaatcc agacatccat tttcaacaac
     1441 gggaaacgat caacccctat tatgataaat tacctgaaat cgttcaaaaa tatatgaaag
     1501 agatcaaccg attacgagga acttcttatg acttggtgac ttattacgga gcagacgatg
     1561 cggaagaagt cattgtttct atgggatcag ttgcacagac gattgaacaa acgattgatt
     1621 acttgaatgc aaacggacgg aaaacaggtt ttttgaatat ccatctttat cgtccttttc
     1681 cagtggaaac attcgtgcaa aagttgcctc atacagtaaa aacaattgct gttctagatc
     1741 gaagtaaaga accaggggca ggtggagaac ctctcttatt agacgttcaa agtgcgttat
     1801 atgattctga acttcgcccg gctgttattg gtggtcgcta cggattgggg tctaaagatg
     1861 tgactcccga ccaaatctca gcagtttttg atgaattgaa aaaagaccca agcatacgca
     1921 aaaaacggtt taccataggg atcgtggatg atgtgactta tcaatctttg gaaccaaagg
     1981 aaagtctgga tttgacagaa ccgcaaacat tccaggccaa gttttgggga tttggatctg
     2041 acggaacagt tggagcaaat aaatcagcta tcaagattat tggagatcac accgataaat
     2101 atgcgcaagg ctatttttat tatgattcga aaaaatctgg cggtctgact gtctctcatc
     2161 ttcgttttgg agatacgccg atacgttcag cgtatcttgt cgagcatgcg gaccttgttg
     2221 cctgtcacac accagcttac ctgcatagct atgacttggt caaaggattg aagccaggtg
     2281 gcattttttt attgaatact ctttggagcg acgaacaatt agaaacgcat ctgcccttaa
     2341 aattaaaacg ttacttggct gaaaataaca ttagattcta tacgatcaat gccatgagat
     2401 tggcccaaga agtcggtttg ggacgccgga tcaatacggc aatggaaacg gcttttttta
     2461 aattagctga catcattcct tttgatgagg tgcttccgct gctaaaagaa gaagccctga
     2521 aaagctatgg acataaatca atggctatcg tagaaaaaaa catacaagca atcgatcgga
     2581 cgaccgaact gcttcatcaa gtcgcgatcc cagaccattg gagaacgttg gagatgcctg
     2641 aaaaaatcaa taaaaggaac actggttata tacaagaaat acttgaacca gtgaatgcac
     2701 aggaaggaaa tcaactctct gttggtacac ttgtagcaaa tggaatgaca actggcgaaa
     2761 tgcctttagg aacggcttct atggaaaagc ggggcgtcgc tttagaagta cctgagtgga
     2821 ttagtgatcg ctgtacgatg tgtaatgaat gtgcctttgt ttgtcctcat gctgccatcc
     2881 gtccattttt agcagatgaa gaggaaatgg aagaagcgcc agaaggattt atcgtgagag
     2941 aaatgcgagg cgcagacggc gtaaaatacc gtatccaagt ttcagtggaa gactgtacag
     3001 gttgtggtct gtgtgtagag gcttgtcccg caaaagaaaa agctttagtg atgaagcctt
     3061 atgaagaaca gaaagaacaa gctgtgaatt gggcttttgc aatgacatta agacaaaaag
     3121 caaatccagt caaaggaaag aatactgtct tgtctacaca attcaatcaa ccgcttcttg
     3181 aattctcggg cgcttgctca ggctgtggcg aaacacctta tgtaaaatta ttgacacaaa
     3241 tgttcggtga tcgaatgatg atcgcgaatg cgacaggctg ttcttctatt tggggcggtg
     3301 cttctcctgc ttctccttat acgaccaatg aatgtggtca agggcctgca tggtcaaatt
     3361 ctttattgga agataatgct gaatttggtt atggtatgct tctagcggca cagactcgtc
     3421 gcgaggcttt ggcaagttca atcaaagaag tattgccaaa agtatcagaa gaactacgag
     3481 aattgatgga agactggtta gtacacatgt ttgagagtga aggaacgcag caaagagcag
     3541 caaaactgca agctgcgatc gaaagcgaac gatcaatttt ccctgaactg gaggatatat
     3601 acaagagaaa ggatctattc gtaaaaaaca gccaatggat gattggtgga gacggatggg
     3661 cttacgacat tggttttggc gggatcgatc atgtacttgc cagcggagca gacgtgaata
     3721 tccttgtttt ggataacgaa gtctattcca atactggcgg tcaaacctca aaagcaacac
     3781 cagcttcggc aatcgctaaa ttttcggcta gtggaaagta tgcttcaaag aaagatctag
     3841 gaatgatggc gatgacatat ggaaatgttt atgtggcgca aattgcttca ggtgccaatc
     3901 aaatgcaaac gatcaaggca tttgaagaag cagaaaagta tcctggtcct tctctgatta
     3961 ttgcctatac gccttgtatc acacatggct tgcttggcgg aatgaaagaa tcgcttaaag
     4021 aagcaaaaga agctgtttct tctggttatt ggtcattata tcgatataac cctacaatgg
     4081 tcgaaaaaaa caagaaccca atgactttgg actacaaaaa acctgatttc tctgctatgc
     4141 ctgatttcat gaggaaacaa gttcgctttt cctcgctaga taacgcaaat ccaaatcttg
     4201 caaaagcttt atttgaaaag actgtccacg acgctaaaac acgtttttac aactatgctg
     4261 cactgacggg agaattagaa aaaatcaaga aacgacttga accagaggaa tctatcgatg
     4321 tacccgttaa gactagaaaa gaaaaaacga cggaccttga agcagaagaa agaagagctg
     4381 ctagaagagc tgccagagca gaacgacgta aaaaaatgga acaagctaca aaagaaaaaa
     4441 aggaaaaata acagaaaaaa tataatgaat aattctctga agtttaaaaa tatttattgc
     4501 agagaaaaat aaaacgcata cttgcaattt tcttgaaaat tatgcgtttt tctcaagaaa
     4561 aatacgagca tatcagcctc taaaattaaa acatatgata tactagagag gaaatctcaa
     4621 aagagaagag aggcttttta tgtcatttca aatcggggaa ctttttaatt tggactattg
     4681 gcagcaaatg ttttccggtg acttgttctc tctgaatttt ttggtgaaca tacttgatat
     4741 tctggttgtc tggtatcttg tatacaaact gatccaactg ctgcgaggca caaaagcgat
     4801 tcagcttctg aaaggtgtag ctatttttat tgttatccga atcgtagcgg aaatcattgg
     4861 tctgcacacc ttgtcttggc tgatgaacca agtcattacg tacggggtga tagctgctat
     4921 cgtgattttt cagccggaaa tccggagagg actggaacat cttggacgca gttcgctttt
     4981 caaacaatca aaaagcgaac agcaggaaga tgagaagatg atcttgtctt ttgacaaagc
     5041 catccaatat atgtccaagc gaaaaatcgg tgcgttgatc actatcgaac gtcatactgg
     5101 attagatgag tatattgaga cagggattgc tttagatgca gatatcactg gggagctgct
     5161 gatcaatatc tttattccta atacaccgct gcatgacggt gcagttattg taaaagaagg
     5221 aaagatcgca gtagcaagcg cctatcttcc tctatcagaa agtatgttga tccctaaaga
     5281 attcgggact cgacaccggg cagctgtcgg tataagcgaa gtcagtgacg cgattaccat
     5341 cgttgtctct gaagaaaccg gggacgtcag catcacgtta gataatgaac tgatggctgg
     5401 cttatcccag caagaatatt tggcaatctt gcgtagagaa ctaattgctg aagagaaaac
     5461 aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt atgactaagg gaggggatcg
     5521 caaatgatct caaaagagaa acggacaagc attctttaca gttttattgc attgctgttc
     5581 agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc caaatacgct ttctggaaat
     5641 gaagcgtata atcaaacagt tacgggggtg ccgatcaaga tttcttatga ttcaaagcgc
     5701 tactatatcc atggatatga aaatgcagtg actgtcaaac tttccagtgc aaaccgagtt
     5761 cagttgaatg cagaggcaaa cgaagataca cggatgttcc gcgtcacagc agatatcact
     5821 aagctgggcg aaggaacaca tgaagttccg ctaaaaattc aaaatttgag cagtgctgtc
     5881 acagcaaaga tcgaaccatc gactattaca gtaacggttg agaaaaaggt aataaaagat
     5941 ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat ctggatatga acttgatgat
     6001 acttccgtta gtcctaaaaa agtctctatc actacaggtg acaaaacatt ggcagaaatc
     6061 aaacgagtag tagctgatgt ggacgcttcg atggtcacag atgatgggat taatagtgag
     6121 gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa gtatcgtctc tgatccagtg
     6181 caagtaacag tgagagctga ttcgatcaag ccaagcaagt cggtacgact ttatgggata
     6241 cagcaaggaa caccagctgc cggggtagaa agctatgatt tcagtttttc aaaactggaa
     6301 gcagaagtaa gcggctctag cgatttgctt gcatcgatcg gtgacagtat tgctgtaccg
     6361 atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa ttgaaatacc agtagaagag
     6421 gggttaagta tcaagcccga atcagttagt gtggagatta cacccgtgtt gcaaacgtca
     6481 agcagtagct ctgcttcgac gagcagtcgg acacaagaaa atacaagtac gagtagaagt
     6541 atttcatctg ctgattcagg aacggaacca tcgacaacaa ccatttcttc cagttcggaa
     6601 tctgggacaa gcagctcgac cacaagtaca gaagaaacat cagaaacaag cgaatcaaca
     6661 caaatgagca gaacaaattc ctcagaaaat taagttaaag gagtaatttt acaactatgg
     6721 gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa caaggaactt acgccagaac
     6781 ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag ccagcatgaa gaagaaggaa
     6841 aacgcccacg cgtgttagta gggcgggata caagaatctc aggtcaaatg ttagaagaag
     6901 cgctgatttc aggactttta tcggtgggta tcgaagtgtt ccagctaggt gtgatctcta
     6961 caccaggtgt tgcttatctg acacgtttgc aaaaagcaag cgcaggggtc atgatttctg
     7021 cttctcataa tccagcacaa gataatggaa tcaaattttt cggtgcagat ggttttaaac
     7081 tagtagatga ccaagagcta gaaattgaag cattattaga tgctgaagtg gatgaattgc
     7141 ctcgtccatc agctgaagga ttgggaactg tagaagaatt tccagaaggc ttattgaaat
     7201 attctcaatt ccttgtacaa acgatcaacg gtgacctttc cggtttgacc atttgtgtcg
     7261 atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt gtttgccgat cttgaaacag
     7321 atttttatac aatgggcaca aatccagacg ggttaaatat caatgacggc gtaggctcta
     7381 cccatccaga acgtttagct gagatggtcg ttgaaaaagg agcagatgct ggactagctt
     7441 tcgatggcga tggcgatcgt attattgcgg tggatgaact tggaaacatt gtcgacggtg
     7501 ataaaatcat gtttatctgt gctaaatact tggcagaaaa aaatcgattg aaaaaagata
     7561 cgatcgttac gactgtcatg agtaacttag gattccacaa agcggtggaa ggaatcggtt
     7621 tgaaagatgt cgttacacaa gttggtgatc gttacgttgt agaagaaatg cgcaaaaacg
     7681 attataactt tggcggcgag cagtctgggc acatgatttt cttagatttt aacacaactg
     7741 gagatgggat gctttcagga attcagttac tgaatatcat gaaacagaca ggtaagaaat
     7801 tatcagagct tgcagcagag gtaacaatct atccgcaaaa attagtgaat atccgcgtaa
     7861 gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa agtgattgaa gaggcagaag
     7921 ctgaaatggg atcagaagga agaatcctag ttcgtccttc cggtacagaa ccattacttc
     7981 gagtgatggc agaagcgcct actgatgaga aagttaatta ctatgtcgat aaaatcgcgg
     8041 cagtagtcaa agaagaaatc ggactggatt aagagatatt ttcataaaaa ataaaagaac
     8101 aaggaccagt gtcagatgtc tgacactggt ccttgtt
//
LOCUS       DAPCMU010000120         7945 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPCMU010000120 DAPCMU010000000
VERSION     DAPCMU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.124"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            127..1218
                     /gene="phnW"
                     /locus_tag="RD744_002200"
     CDS             127..1218
                     /gene="phnW"
                     /locus_tag="RD744_002200"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDU0558183.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1211..1339
                     /locus_tag="RD744_002201"
     CDS             1211..1339
                     /locus_tag="RD744_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDU0558184.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1343..1888
                     /locus_tag="RD744_002202"
     CDS             1343..1888
                     /locus_tag="RD744_002202"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDU0558185.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2342..4033)
                     /locus_tag="RD744_002203"
     CDS             complement(2342..4033)
                     /locus_tag="RD744_002203"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDU0558186.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(4453..5400)
                     /gene="arcC"
                     /locus_tag="RD744_002204"
     CDS             complement(4453..5400)
                     /gene="arcC"
                     /locus_tag="RD744_002204"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDU0558187.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(5515..6534)
                     /gene="argF"
                     /locus_tag="RD744_002205"
     CDS             complement(5515..6534)
                     /gene="argF"
                     /locus_tag="RD744_002205"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDU0558188.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(6625..7854)
                     /gene="arcA"
                     /locus_tag="RD744_002206"
     CDS             complement(6625..7854)
                     /gene="arcA"
                     /locus_tag="RD744_002206"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDU0558189.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2259 a         1620 c         1465 g         2601 t
ORIGIN      
        1 catcattttt tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata
       61 ttggatgtta acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga
      121 aatcatatgt ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat
      181 gctatgctga tcgatcattg tacttgggat caagaataca aagaaattac acaatggata
      241 agaaaaaaat tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg
      301 caaggtagca gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat
      361 actgtactga tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt
      421 ctgaaactga actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg
      481 attacacagc tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag
      541 actacttccg gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat
      601 cttttgttta tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa
      661 ctagggattg attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt
      721 tcttttgtac ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg
      781 tcacttgatc tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc
      841 acttccccta cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa
      901 ggaggaatcg aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa
      961 atgacagaga tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct
     1021 tttctttacc ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa
     1081 aatggatttg tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat
     1141 attggcgaga tctatccgga agatattttg gaactgacag caattatcaa agactataag
     1201 gagaacgtta atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg
     1261 ctgtatggct cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac
     1321 tgacaaagaa atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct
     1381 ttccctccct tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa
     1441 agaggtggag gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca
     1501 ttctaccttg aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa
     1561 tattggttcc acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa
     1621 aaaagaaagg tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg
     1681 accgtttcct tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga
     1741 agtaatcaaa gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt
     1801 gacagtaggt gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa
     1861 attagagatg gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc
     1921 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     1981 atgaatccag aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa
     2041 gtaataaaaa cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     2101 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga
     2161 cttttgtcac agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta
     2221 aacggtgttc attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc
     2281 tattttctct tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt
     2341 cttacatctt atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag
     2401 tcactgcata aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg
     2461 tatgagcata gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg
     2521 acggttcgta tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa
     2581 ccacttccca gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc
     2641 ctgctttttc caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt
     2701 ctccttcaaa tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt
     2761 tcaaatcgtg gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt
     2821 ccaaatcagg gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata
     2881 cttcttctaa taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga
     2941 tcaaaccaaa tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga
     3001 cagctttcaa ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt
     3061 tgaagtcgta agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg
     3121 tcgcaccgtc tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga
     3181 tctctgcacc tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat
     3241 ccatcttgtc attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat
     3301 catagatttt attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag
     3361 cctctttatc gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg
     3421 gttctgtttc agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt
     3481 ctgctttaac agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact
     3541 gtgttcccca gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa
     3601 tgaaaccgat cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg
     3661 caatattagg agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc
     3721 cataatgttc tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat
     3781 tcatgaaaaa gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga
     3841 ttttttctgc taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg
     3901 aaaaggctgg aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct
     3961 cgacttgttc cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat
     4021 ctttattatt catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc
     4081 tctagcgacg gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc
     4141 caattgtcct aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa
     4201 cagaattctc caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat
     4261 tcgctagcca agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact
     4321 ttcttgaatt atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca
     4381 gatggcctaa aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa
     4441 agacttaaca aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc
     4501 gatattttct aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat
     4561 agctgcttct actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg
     4621 tttcatttct gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac
     4681 atacacattg tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa
     4741 tttttctgaa gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt
     4801 ttctacaaca ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag
     4861 cgtttcgatg acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct
     4921 ccatccacgt ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt
     4981 ggcttcttct tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc
     5041 attttcatcc acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc
     5101 cttcagtact tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc
     5161 aacacatgta tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat
     5221 caataggtta ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc
     5281 gataaatttc actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact
     5341 tgcatccgta gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat
     5401 ttcattcgtc ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt
     5461 tgtgattttc cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac
     5521 gcgagggata aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg
     5581 attttctgct tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc
     5641 catttcagtg atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg
     5701 gaaagctggc aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat
     5761 ttccatattg atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc
     5821 acccattgat acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac
     5881 atcgtcggta atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac
     5941 gatttcttct tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat
     6001 tgcacctgtg actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac
     6061 agtgatacct tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg
     6121 tgttggatgc cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag
     6181 ttcctctacc attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag
     6241 tactttagct gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag
     6301 atattcagga tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt
     6361 agatgttttt tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc
     6421 acgttttttc aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt
     6481 tgtaaagtct ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa
     6541 cattctcctt tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca
     6601 gtaagactgc cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat
     6661 gcagcgtgga ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat
     6721 gccatagcta cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc
     6781 tggagcgatt gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc
     6841 atctccatta ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt
     6901 ttcgtgtaag ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa
     6961 gacatccatc ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc
     7021 taccattgta aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc
     7081 caacactttt tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc
     7141 tttcgcatta gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc
     7201 gtctccgcct tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt
     7261 tgcaaatctt ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc
     7321 ttcaaaagtc atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt
     7381 aaagtacaag tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc
     7441 atatagactg ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc
     7501 taccatttct tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt
     7561 cgatgcaata tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc
     7621 gtcaatcgct tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa
     7681 tgtcttcgca aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa
     7741 caacagacga tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag
     7801 taaaactgtc tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg
     7861 ttgcctcctt ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa
     7921 ttttgtctca ttcaaaatga gacat
//
LOCUS       DAPCMU010000121         7932 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPCMU010000121 DAPCMU010000000
VERSION     DAPCMU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.125"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            22..723
                     /locus_tag="RD744_002207"
     CDS             22..723
                     /locus_tag="RD744_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDU0558190.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1332..2348)
                     /gene="tsaD"
                     /locus_tag="RD744_002208"
     CDS             complement(1332..2348)
                     /gene="tsaD"
                     /locus_tag="RD744_002208"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDU0558191.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2345..2809)
                     /gene="rimI"
                     /locus_tag="RD744_002209"
     CDS             complement(2345..2809)
                     /gene="rimI"
                     /locus_tag="RD744_002209"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDU0558192.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2816..3358)
                     /gene="rimI"
                     /locus_tag="RD744_002210"
     CDS             complement(2816..3358)
                     /gene="rimI"
                     /locus_tag="RD744_002210"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDU0558193.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3342..4064)
                     /gene="tsaB"
                     /locus_tag="RD744_002211"
     CDS             complement(3342..4064)
                     /gene="tsaB"
                     /locus_tag="RD744_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDU0558194.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4255..5235)
                     /locus_tag="RD744_002212"
     CDS             complement(4255..5235)
                     /locus_tag="RD744_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0558195.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5259..6743)
                     /gene="galT"
                     /locus_tag="RD744_002213"
     CDS             complement(5259..6743)
                     /gene="galT"
                     /locus_tag="RD744_002213"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDU0558196.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6755..7744)
                     /gene="galE"
                     /locus_tag="RD744_002214"
     CDS             complement(6755..7744)
                     /gene="galE"
                     /locus_tag="RD744_002214"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDU0558197.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2231 a         1704 c         1403 g         2594 t
ORIGIN      
        1 caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc
       61 agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat
      121 actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa
      181 cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact
      241 tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga
      301 gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct
      361 gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa
      421 aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc
      481 gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca
      541 aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc
      601 ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc cgggctgtta
      661 gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa
      721 taacgcgaac aaaaataagc agtgaccctg ataaatatca gggtcactgc ttattttcct
      781 ccctaaaact tctgaaagat ccggcagttt tagatcactg gacaagactc aaagattttc
      841 tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgtta gattaactag
      901 atgaagcgtc ttgtcctcat ccgatctaga ttattcgata ttcctcgaca ataaacgatt
      961 actcagtgag ggcaatcaga ggaacggata atgataaata agctgtaaaa aatagcaaaa
     1021 taacaataat gattaaaagt atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc
     1081 tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa
     1141 acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac
     1201 gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc
     1261 aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagctttt
     1321 tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca
     1381 aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca
     1441 caaagtcgaa gagggggaat cactagttct acctcaggga gtttagctgc caaagcatcc
     1501 tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctataat cagttgcttg
     1561 actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct
     1621 tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc
     1681 accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg
     1741 atcatcgctc gcggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct
     1801 tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct
     1861 gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat
     1921 tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga
     1981 cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc
     2041 caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca
     2101 taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct
     2161 tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg tactacgcca
     2221 ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca
     2281 tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct
     2341 tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg
     2401 gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt
     2461 ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt
     2521 gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg
     2581 caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac
     2641 agatctggtt atcttcagct aaaagcagat aatcagagtg aggctgttgg atgtctgcca
     2701 aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata
     2761 cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact
     2821 cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt
     2881 ttttgatcgt tgttgcttga aaccccaatt tccgataaag tctttgtgca tcttgattac
     2941 tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt
     3001 cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg
     3061 tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat
     3121 caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt
     3181 ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat
     3241 cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgact gttttttctg
     3301 tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa
     3361 ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac tcgttttaaa
     3421 tagcgaggaa gaaaagcttg tttctctgca actggttcaa ctaaagcacc taattgagcc
     3481 acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat
     3541 atttccttta tttcagagaa gaatttgtga caatcactcc cgacgaaata aatagcttca
     3601 ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca
     3661 gtttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca
     3721 tcaaacatgg gcacaataat attatccaca ccgatgcaat tagccgcgat ggttttcaga
     3781 ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca
     3841 cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct
     3901 tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg
     3961 tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg
     4021 atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct
     4081 gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca
     4141 aaggcaaaac tagtacaata atacatgatt atctgctggc gggcaaattt tgccccttcc
     4201 tgaaacacaa aaaagctgcg gtatcactcc attttcccac agcttttcca tcttttattt
     4261 cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa ccggtgcttt
     4321 ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt cggtataaac
     4381 ttttacagta gaaagcgagg gcgttacata tttcgctaca ctgacatcat tgaagcccat
     4441 cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca gtgcgccaat
     4501 cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg tttttaaata
     4561 ttgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa ttggaccttc
     4621 gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat cttttagccg
     4681 ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt gcgctttatg
     4741 ggagacaaag tgttcaatga ctttttgaac acttgactta aagtcgacaa ctagagagtc
     4801 gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg atcctttcat
     4861 ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg ccggaagatc
     4921 actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg cctttttttc
     4981 gattcctagg cgaatagata aataataaag atcttcaagc tcttccgtat cgtcgtacca
     5041 ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt tcgtataatt
     5101 taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg aaagatcagg
     5161 atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact gcgcaatgtc
     5221 tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt
     5281 tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt
     5341 gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt cgtcaaatca
     5401 ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat ctggttttct
     5461 tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg tggtaaaaca
     5521 gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg gacatcttta
     5581 tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg aaggacaagg
     5641 tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta
     5701 ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt ccatttttcc
     5761 aatactgagt tagctgcttc aataagcaag tttttctctg ttgattgcaa acggatcaca
     5821 gacatcggcc atttcacgac tcctgcgatg acttgcggat aatcttctag tttgataagt
     5881 ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata atgatcatga
     5941 gaaagaatcg atcctccaac aattggtaag tcagcattcg aaccaacaaa gtaatgaggc
     6001 aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt catcgggcgg
     6061 tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataagggga atattggaat
     6121 ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg ggctggatag
     6181 ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg atagtctact
     6241 tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt agataaattg
     6301 attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt ttttgcgatg
     6361 gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc agttgcttcc
     6421 tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg
     6481 agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca
     6541 ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc ttccttaact
     6601 ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag acgattctgt
     6661 aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt ggcaaaatca
     6721 caaatcgttt gactgattga catatctcat tcccctattc ttcataacca tttggatggc
     6781 tgacatgcca atcccatgct gttttgataa tcgctttgat atctgtgtat tcaggtttcc
     6841 agcccagaat ctctctcgct ttttcacttg aagcgactaa tcggctagga tctccagctc
     6901 ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct gcttctagca
     6961 tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt
     7021 ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact tgaacataat
     7081 ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc
     7141 tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt gtatgatctt
     7201 ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc
     7261 gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat ttactttcac
     7321 catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct ttcggctctc
     7381 cataagttgc tgcagtagaa gagaatacga ttttattcac gttgaactcg tgcatgactt
     7441 caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt ttttcgacag
     7501 attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt tccttttcaa
     7561 atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga gcatctggat
     7621 gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga tacccctttt
     7681 gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt actaaaattg
     7741 tcataaaaaa cactcctttg attagtaaaa ttgtgtttgt tgattctata taatttaaaa
     7801 aacgaagttt acttttatgg tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta
     7861 aatttataat aaaacattta gtaaacattt tcaagggttt tttgcaaagg tttacaaaca
     7921 aacgtaaaac ta
//
LOCUS       DAPCMU010000122         7874 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPCMU010000122 DAPCMU010000000
VERSION     DAPCMU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.126"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(21..995)
                     /locus_tag="RD744_002215"
     CDS             complement(21..995)
                     /locus_tag="RD744_002215"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDU0558198.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="RD744_002216"
     CDS             complement(1075..1950)
                     /locus_tag="RD744_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDU0558199.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="RD744_002217"
     CDS             2080..3987
                     /locus_tag="RD744_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDU0558200.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="RD744_002218"
     CDS             complement(4528..7806)
                     /locus_tag="RD744_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDU0558201.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2219 a         1664 c         1463 g         2528 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtca
//
LOCUS       DAPCMU010000123         7652 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPCMU010000123 DAPCMU010000000
VERSION     DAPCMU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.127"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            20..2347
                     /locus_tag="RD744_002219"
     CDS             20..2347
                     /locus_tag="RD744_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0558202.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2473..2802
                     /locus_tag="RD744_002220"
     CDS             2473..2802
                     /locus_tag="RD744_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDU0558203.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2842..3846
                     /locus_tag="RD744_002221"
     CDS             2842..3846
                     /locus_tag="RD744_002221"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDU0558204.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3843..4289
                     /locus_tag="RD744_002222"
     CDS             3843..4289
                     /locus_tag="RD744_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDU0558205.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4439..5254
                     /locus_tag="RD744_002223"
     CDS             4439..5254
                     /locus_tag="RD744_002223"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDU0558206.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5460..6632
                     /locus_tag="RD744_002224"
     CDS             5460..6632
                     /locus_tag="RD744_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDU0558207.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6794..7288)
                     /locus_tag="RD744_002225"
     CDS             complement(6794..7288)
                     /locus_tag="RD744_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558208.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2573 a         1451 c         1525 g         2103 t
ORIGIN      
        1 ttttttattg agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat
       61 atgtcaaaaa cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc
      121 cattatgatt tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt
      181 aaacgtttat acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc
      241 accctattct gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac
      301 ctcaaccaca tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa
      361 agcagcaggg gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc
      421 ggccactgcg aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa
      481 agatggagtc attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg
      541 cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg
      601 ctccacagta aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac
      661 cgctggaaat acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc
      721 ctcaacatgg ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca
      781 gttagcaaat gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg
      841 taagggaaat gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa
      901 tcggacagga actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg
      961 gactacatta tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac
     1021 gggctattct tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac
     1081 ggtgacctat acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca
     1141 gatgattgct ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac
     1201 gactaatacg cctactatca acggatatac ctttacagct gtttcagcga tagaaacaaa
     1261 gaaccaatca gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa
     1321 ggacgcaaat ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt
     1381 atcaatcaat gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa
     1441 atatgccttt aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga
     1501 tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac
     1561 tgcgcctact gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa
     1621 actagctgcc agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat
     1681 taagcaaatc agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg
     1741 tgtcccgtca atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca
     1801 agtggcactt agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa
     1861 tgctggcagc tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa
     1921 tcccaagttg tcatttactg atatagccaa cacatcagga acttatacca tctataaagc
     1981 taacgcagtg attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat
     2041 ttcggttgga aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct
     2101 aacagttaac agccggacag atattggatc acaaatagta acgattagtt taggcgaagg
     2161 aagtgtaaac aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc
     2221 gactctcttg cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact
     2281 gatctttatt ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca
     2341 gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta
     2401 ctggtctttt ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact
     2461 ttgcataatc gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata
     2521 aatatcccgg ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat
     2581 gatgtctcgg atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa
     2641 agaaaactag cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca
     2701 gtagacgagg aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt
     2761 gacgctgttt accgatttac ggaaataaag acttcagatt aagtaaaaga aaaatcagca
     2821 ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac
     2881 ttgtatccaa agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag
     2941 aataagcaga cagattttgc agatggggat aaagtccctt atctcatcac cgtaacaatt
     3001 cctgagaaca tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta
     3061 tggctagaac cagaaacgat cgattttttg attggtggag acaagattca tacatttcac
     3121 acacaaaaaa taaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt
     3181 gctggaaaaa aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg
     3241 acatttgata acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc
     3301 atccaaacag gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca
     3361 gatgctcaat ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat
     3421 cattgggtag ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca
     3481 ttttctgtcc atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa
     3541 ggatatatat tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg
     3601 cttcatccta taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga
     3661 aaaacgccag ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt
     3721 ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt tagggttatt gctagtcctt
     3781 tcaacaggga cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata
     3841 aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc
     3901 ctttcgttct cggcctagga actgcagagg tttctgcagc cgtatcacct acaccagaaa
     3961 atgttacggt taatttgcat aaattgaaat tcacttcagc accagaaaat caaataaaca
     4021 acggaacgga attgacattt ccgaatagcg aaccgttgaa cggcgttgaa ttcaacgtat
     4081 atgacatcac tgctacgtac tatccaagta aagacacagc agttccagca gacgctacac
     4141 ccttcgcaag tgtaacgact tctggtgaag gattagctaa cctgactctt ccaggtaaat
     4201 ctgatgggaa agatgctgta tacgtctttg tagaaacacc aaagcccggt gtcgaaacgt
     4261 cgccaaatat cgtgttaagc ttaccctaga ccctgacaaa cctttagaca cgattcacct
     4321 atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg ggaacttttc
     4381 aggggtagat aagttagcag gggcgcaatt ccagatcaaa aattcagaga atcaattcgt
     4441 gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt cagcgttgac
     4501 attcacaact gatactgaag gatccatcag tgtcaatgga ttaatagacg gcaattatac
     4561 tttagttgaa acaaaagcac cagatggtta tcaattaacc aatccacaaa aagaaactac
     4621 ttttatagta aaaaacggaa aattgcaaca aacaaccggt atcatccatg gcgcagatga
     4681 agccaatctt gggaatccaa acggtgtagt caatcgggaa aatacaacta cggataacac
     4741 gattactgta gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac aagatgtgaa
     4801 cacgcaagaa aaattagccg atgcagaatt ttctattcta agagcaaaag atccaactgt
     4861 taaattaatg aaaaaaatag caaaccttgc ctcaggagaa tacccatatg cttggtcaga
     4921 tgaagtcaca ggttctgact gggaagaagt gaaattgatt tctgattcag aagggaaatt
     4981 ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa gaaacaaaag caccagaagg
     5041 ctacgcatta cctaagaatg aagctgcgta cactgaattt acagtagatg atgatccaac
     5101 aacttctgat gaaacaacga tcgataacca acctaaagga atcttgcctt caactggtgg
     5161 tatggggatc atcgtattca tcttggtagg tactgcttta gttggaggag cagtgatcta
     5221 tttcaaaaaa cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa atcatacgat
     5281 aaataaacga tagtgattca aatggggaga aaggttgtga caagtggtcg gtctcttggt
     5341 attcgctcaa caaacggcaa aataggagga actattttgc cgtttgcttc gctaatactc
     5401 aggtcaaggc gctgatttca caacctcttc atatacatat tagaaaatag gtggacatga
     5461 tgaataaaag cagaataaga atactggaca tcttcatggc aatcatttta gtcgttggga
     5521 tcgggatctt ttcttatcct tttgtagaag attcactgaa tgattttctt gcccagcaaa
     5581 tgatcatcca ttatcaaaag caagcatcga ataagaattc agaggaaata aaaaaacagc
     5641 aggaaaaaat gacaaagaaa aaccaacagt tggcagagca aaacgtatca ccagggatag
     5701 gcagtttcaa ccaagcagtt gatgcaaaag cattaaaaga tttacctagt aatgcctttt
     5761 ttatggaaca tatgcttgga gtcatcgaaa tcccaaaaat caatgtcagc ttacctatat
     5821 tcgatcagac aacggaaata tttttgcaaa aaggaacgtc attattggaa ggaagttctt
     5881 atccgactgg cgggaaaaac acccatgccg ttctttctgg gcatcgtgga ctcccagaag
     5941 caaaattatt tacagatttg cctaagttga aaaaaggaga ccagtttttc atccagatca
     6001 atggaaagac attggcgtat caagtagaaa aaatccaagt ggtcttacct gacgaagtag
     6061 attctttggg tatccaaaaa ggcagagatc tcgtgacttt gcttacctgc actccttata
     6121 tggtcaatac acatcgactg cttgtgacgg ggcatcgtat tccttatcaa gcaaaagagg
     6181 caaaaaaagc gatccaagga attgatcaat ggaaaaaatg gaaatttttt atttggttta
     6241 tcggtatact acttggatca atcggcttag tttggctact catcgcttat ttagattctt
     6301 tggccatcgc taagcgaaac tatcctttat ctttttatgt aaagaacaca aacggacgac
     6361 caatcgaagg aatggtcttt tctgtaaaaa cgctcaacgg caaacactat attactagag
     6421 aaaaagttcc atttgtgaaa gcttctgatg aatacggact agtgagattt tctgatttaa
     6481 aaggcaggaa ctatcgcttg caacatgagg agctcctgct taaaatccat gtaaaacata
     6541 aacattcgaa acagttttca atgaaattga aaaaaggaag atataaactg cgaaaagaaa
     6601 aagaggtgta ctatttgatt gagaaagaat gaatgaagat gtccaaaaaa gcagagggaa
     6661 cagactaaat gaaaaaagag agaagctgac gaaatggacg tgtaaactgc tatttttggt
     6721 ttactatctg gaacgccagc ttctattttt ttatgtttca gaagaatttt attattatag
     6781 tataactttt gaattagttt ccataacatt tggagcaagg ggttaaacca cgagcttgag
     6841 cttcgcttaa agtggcaggg gtatacgtac catttccaca tttatgtgta tggtattttg
     6901 aaccggtcgg tgtgacatag acagtttgtt cattattttg ctcaggagct gcggcttgtg
     6961 cctgctgaga agcctgttca gctgctgctt gctgtcgcgc ctgttctgct tgttgctgtg
     7021 cgattgcttc ttgcctcgcc tgttctgctt gttgctgtgc gattgcttct tgttgcgctt
     7081 gttctgcttg ttgtcgtgca atttcttctt gctttttccg ttcttcttct gcttcttttg
     7141 ctttgattgt ggaatcaatt gcagcaagac ggcttgtcaa ctcttgtttg ttttcaggta
     7201 gcgcttggat agctgctgaa gcagttgcat agttttcttt tgtaggattt gcttcagctt
     7261 gtgacaaagc agattccgct tctgtcactt ttttttctag tgcagctttt tccgcttttt
     7321 gtttctcttc ttcttttttc ttttcaattt gttctttcga tgaatctgcc gtactttcgg
     7381 agatcgtggt actgctgctg gaagcctgag ctgaacttgt gctttgagta gtagacgtag
     7441 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caacgcaact aattttttca
     7501 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
     7561 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
     7621 tgttgataca cgggattttt tgctatagtt ct
//
LOCUS       DAPCMU010000124         7620 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPCMU010000124 DAPCMU010000000
VERSION     DAPCMU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.128"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(59..787)
                     /locus_tag="RD744_002226"
     CDS             complement(59..787)
                     /locus_tag="RD744_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0558209.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="RD744_002227"
     CDS             complement(800..2080)
                     /locus_tag="RD744_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDU0558210.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="RD744_002228"
     CDS             complement(2221..2823)
                     /locus_tag="RD744_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDU0558211.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="RD744_002229"
     CDS             3068..3274
                     /locus_tag="RD744_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558212.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="RD744_002230"
     CDS             3586..4305
                     /locus_tag="RD744_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDU0558213.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="RD744_002231"
     CDS             complement(4386..5069)
                     /locus_tag="RD744_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDU0558214.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="RD744_002232"
     CDS             5287..7473
                     /locus_tag="RD744_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDU0558215.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2728 a         1284 c         1437 g         2171 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tcggaaaagg gaggtgtgac
//
LOCUS       DAPCMU010000125         7320 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPCMU010000125 DAPCMU010000000
VERSION     DAPCMU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.129"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(20..853)
                     /locus_tag="RD744_002233"
     CDS             complement(20..853)
                     /locus_tag="RD744_002233"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDU0558216.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(1045..1749)
                     /locus_tag="RD744_002234"
     CDS             complement(1045..1749)
                     /locus_tag="RD744_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0558217.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(1772..2398)
                     /locus_tag="RD744_002235"
     CDS             complement(1772..2398)
                     /locus_tag="RD744_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDU0558218.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            2724..5099
                     /locus_tag="RD744_002236"
     CDS             2724..5099
                     /locus_tag="RD744_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDU0558219.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(5123..5614)
                     /locus_tag="RD744_002237"
     CDS             complement(5123..5614)
                     /locus_tag="RD744_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDU0558220.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            5801..6721
                     /locus_tag="RD744_002238"
     CDS             5801..6721
                     /locus_tag="RD744_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0558221.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            6875..7219
                     /locus_tag="RD744_002239"
     CDS             6875..7219
                     /locus_tag="RD744_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDU0558222.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT         2388 a         1320 c         1423 g         2189 t
ORIGIN      
        1 agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa atgataccaa
       61 ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag cttagtaaga
      121 atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga agaacaacag
      181 tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc aagccgcttg
      241 cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca aaacgcgaag
      301 caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg ggaacaagtg
      361 ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca ttcagagaag
      421 tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca ataccgtaaa
      481 cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac caatcatcca
      541 aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac caaagtgtcc
      601 acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac ttgtgaaaat
      661 aaagaacgac gacagccaag atcgccccta attggataac gacattgaac atcgacataa
      721 attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga ccagtactac
      781 tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct ttgataatat
      841 ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt ataccgttat
      901 tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat aaagcagata
      961 agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt ttatgattct
     1021 ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg ttttcccgtt
     1081 atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg cataagtgac
     1141 ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat cgatcagatt
     1201 aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac caccattttt
     1261 ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca atgtttcata
     1321 agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt gttcaaaaga
     1381 aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag ggatcacacc
     1441 ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc taatcctttt
     1501 ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa tacgggcagt
     1561 ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat tttttaaccc
     1621 gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat cacaccagcc
     1681 ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat aaagtgtagt
     1741 ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga tttgtccttt
     1801 tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat tcatcaggta
     1861 atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct gtatacagat
     1921 cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa tcatcaaaaa
     1981 taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa gacatgtttc
     2041 ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata acatcagcaa
     2101 tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc catttttgct
     2161 ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc actgcaccga
     2221 tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt gtttttttga
     2281 tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt gcttcttcgt
     2341 tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg atatgcatgt
     2401 gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat tcattgctag
     2461 gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact atatgaaaag
     2521 tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat atgtccttgt
     2581 tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct aatcagcagg
     2641 gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt tcaggtaatc
     2701 ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg cggcacttag
     2761 tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac catactgcat
     2821 tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac tatgaaaaag
     2881 aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt gaaggccgtg
     2941 cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa acctccgaac
     3001 cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa gcaagggtgt
     3061 atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa ttgacttact
     3121 tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga gagcagttag
     3181 aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt caagaaaact
     3241 ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat gagtatagga
     3301 aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa cagaaattgt
     3361 ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc gctttgaaag
     3421 ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca ataactagac
     3481 aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta aaaagtgtca
     3541 ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat ccagatcagt
     3601 gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat cttttgaatc
     3661 atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat acactttgtc
     3721 ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg gattacaatt
     3781 atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa aacgaggact
     3841 atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag atgtattcgg
     3901 cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag gaattcaaaa
     3961 aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga gaaatagcaa
     4021 aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta gtaaaggcag
     4081 gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag cacccatcac
     4141 aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc agtgaatttt
     4201 atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata gtaaaagagt
     4261 tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa agcagtattc
     4321 ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg ggacaaaaaa
     4381 gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt ttcatcgcaa
     4441 attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta gtagagacga
     4501 ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct tcttatcaat
     4561 atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat gtgctaatcc
     4621 aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt caaacaaatc
     4681 ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa ggaatcgatt
     4741 tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg cagatgaatc
     4801 atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga tttgcttacg
     4861 cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg attcgtggca
     4921 tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt tcttatcgac
     4981 gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg gaattggaag
     5041 aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac gaggaatgac
     5101 agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg attggtaagt
     5161 caagtttacg taataaagca atcacagctt gtagatcatc acggttctta cctgtcactc
     5221 taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg atagctgtag
     5281 tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt tgtcgagctt
     5341 tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc acatttcttt
     5401 tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca tcatcgctca
     5461 atacaactag attgctgttc tctaatttga tttctactgt agaatccttg aagtcaaagc
     5521 gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct gtattgactt
     5581 ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata tacatataaa
     5641 tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg tttgctaaaa
     5701 ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat tcttacattt
     5761 ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt atcataaaaa
     5821 tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg cggtcgcttt
     5881 aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga tcgctcaaat
     5941 catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa catttatttt
     6001 tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac gagcaacgat
     6061 ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc cagtcggaca
     6121 gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag gtcttggcgt
     6181 aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct ctcttgtact
     6241 ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg gtatcattgt
     6301 agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta tttccattta
     6361 tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa cagcaatgat
     6421 cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg tacgtggaat
     6481 gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga tcatgatggt
     6541 catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg acgacgatgc
     6601 atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg aaaacgaaca
     6661 aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac aaaaaaaatg
     6721 aggaatcagg tttattttag aataaattta atctattaca tctaataaga acatttttat
     6781 aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa aaaagagtat
     6841 gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc aagaacggtc
     6901 tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa tggtgattga
     6961 tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag aattcaatcc
     7021 tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc cattagcaga
     7081 tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa cgaaagcaga
     7141 agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt atgcacggat
     7201 tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa taggaaaaac
     7261 aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg tttataaaat
//
LOCUS       DAPCMU010000126         7274 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPCMU010000126 DAPCMU010000000
VERSION     DAPCMU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.130"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(268..753)
                     /locus_tag="RD744_002240"
     CDS             complement(268..753)
                     /locus_tag="RD744_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558223.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(892..1107)
                     /locus_tag="RD744_002241"
     CDS             complement(892..1107)
                     /locus_tag="RD744_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDU0558224.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1331..2131
                     /locus_tag="RD744_002242"
     CDS             1331..2131
                     /locus_tag="RD744_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0558225.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2150..4018
                     /locus_tag="RD744_002243"
     CDS             2150..4018
                     /locus_tag="RD744_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDU0558226.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4011..4502
                     /locus_tag="RD744_002244"
     CDS             4011..4502
                     /locus_tag="RD744_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDU0558227.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4490..5044
                     /gene="srlA"
                     /locus_tag="RD744_002245"
     CDS             4490..5044
                     /gene="srlA"
                     /locus_tag="RD744_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDU0558228.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5062..6057
                     /locus_tag="RD744_002246"
     CDS             5062..6057
                     /locus_tag="RD744_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDU0558229.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(6199..>7274)
                     /locus_tag="RD744_002247"
     CDS             complement(6199..>7274)
                     /locus_tag="RD744_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDU0558230.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2470 a         1178 c         1454 g         2172 t
ORIGIN      
        1 agtaaattct tctatccaaa tatagaaaaa ttgaatctag aaaaccgccc gacaataaat
       61 ttcattaact cagttgaccg aaaatgaagt agataccaaa aatttatcaa gagagcaagt
      121 agagaagtga gtatattcat aagagaaata gctgtaacga attattaggt taatgcaaat
      181 gcttatttat actccgtata actttctcag aaaaaatgat accaataatg acttgctagt
      241 actgtaaatc cgtataatat catctaatca aaatggtgtt ccatttgcat ctgtcatcgt
      301 aatcgatcct tcgggtgaca ctcgataaaa accaacgctt ccagatcctc cttgggaacg
      361 gatttgtttg ctggaagctg tgaacaaata atctcgatca attttttgat aaaagctaat
      421 aacaatatcg tcactttgta attcggaacg gtgggcaata agaaatagaa gtgctttgtc
      481 agagttttct aatggaagag aaggtacagt gctttcttgc atttcagatg ttgaagaaat
      541 gctaaacgac tgaatacttg attggctatg ttgcgaatct acattaccag tagaagtatt
      601 agtaatggaa gtagtagaag tacttttagt tgaaccaacc tcagttgttg aaatggctgc
      661 tgaagattct ttaacagaat tctttgtttt atatccacag gcagaaaaaa gtattacagt
      721 tcccaacaag cctgcgtaaa tccctttatg catgatagct cctcctaaaa tagattatct
      781 ttattcagta tatcacttaa acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc
      841 aataaaggga aggttcatga agataataac ttttttaata attatagctt tctaatatct
      901 ttcatcaact tttctagtaa tcattgggaa aaatttcaag cagtagatcg cacaaatacc
      961 tacgacgata taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac
     1021 taaatcaagt tcgaacaagc ctaccaacag ccagttcaac cctcccacaa tcaataaagt
     1081 taatgcaata gcatctaaag ttttcattag aattctcctt tcttaaatga taaaacaatt
     1141 atatatcaaa aagagaaaca ttgcttatat aacgctcagg aattttagca atctatgcaa
     1201 aaagatgaaa attttttttg ctcttccatt agtacacatt ttttagctga agctgtaaat
     1261 ttcatgtctt aatttttttt agtgagcttt tatactggat ttgtaagaaa aaagaaggag
     1321 aggatcacat atgtcggatt ggttggatat tgcaggaaaa gtagtaattg taactggagg
     1381 ttcctcagga attggaagaa gtatcgtcga aaatttgttg aagcagaatg cacaagtagc
     1441 gaattttgat gtaactgaat gccgtataca gcatgaaaac ctactatctt taaaagtcga
     1501 tgtgtcttct aaaacggaca taaaagaagg gatatataaa gtaatgaaac attttaagac
     1561 gatcgatggt ctagtcaata atgcaggaat aaatattcct tctcttctaa tagatagaaa
     1621 tcatccaaaa agcaagtatg aattatcaga acaagttttt gataagatga tcgctgtcaa
     1681 tcaaaaaagt gtatatttga tgagccaagc agttggaaga atcttggtgc aaaagggatc
     1741 aggagtgatc gtgaatcttt cttctgaatc aggattggaa ggatcagaag gacagagctg
     1801 ttatgctgct acaaaagcag cgatgaacag ttttactcgt tcttgggcaa aagagttagg
     1861 aaagaggaat gttcgtgtgg taggtgttgc accagggata ctggaagaaa ccggtttgag
     1921 aacacaagaa tatgaagaag ctctttctta tacgagaggt ataagcgtcg aacagctgag
     1981 aaacggttac agtcgaacaa gtacgatccc tctaggaagg agtggtaagc tgcaagaagt
     2041 ggcggatcta gtctgctatt atctttcaga aagatcaagt tatatcactg gagtcacgac
     2101 taatatttca ggaggtaaaa caagaggata aaggataggg gggacgcacg tgacgctagt
     2161 aaacagatgg taccaattat tgaaattact agtcactcac aagaaagttt caatccagga
     2221 agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa aaagatattg gggagttgaa
     2281 tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac aaacattttg aacttgaaat
     2341 ttatgatttt gatttttttg atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt
     2401 caattcggca tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa cagaggattt
     2461 cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc agaggaacag tggtcaagga
     2521 tctaaagaca ttaaaagaga tgatcaatga ttttgatgta aagattacag gcacaccaaa
     2581 taaaggacta agaataatag gagatgagct agaattacga ctacttttat tttattttgt
     2641 ttctccctac ttttcagaga cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa
     2701 acattttcag aagaagacga atgctagtaa acaagaaatc acccttttaa cgaaagttat
     2761 cggtatttct ttgaatagaa tctctagtaa ctatctgttg aagaagccca tcaatcgtta
     2821 cagtgggtgt tttgaatata tccaagaatt caatgaactg atcaatcagt tggaagaaat
     2881 ctatgaacta actttgagcc aatatgaaaa agattttatc agttttcctt tgaacatcat
     2941 caacacaagt ataacgatga taaggaaagc taactctgat ttgcgcttat atttcgaccc
     3001 catgatgatg cgtattcgac gattagatct cattgatctg gatgagtcct tcttatatca
     3061 agagatgaaa cagcacctct ctttattgat aaatcgggta atttttcgtt atcgattgac
     3121 tgatttattt tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga
     3181 atgcatacaa gcattagaag ctatcttgca acataagatc tctaaagtag agatcagcta
     3241 cctaacgttg tatttggagt tagcatttcg atatcaaaaa gagcattttt cctctaaaaa
     3301 agtagccatt gttcatacga caggaaaagg aacagcatta atgatccaaa gacaaatcaa
     3361 aaggatattg gggagggata tacaaataac acatgtttca gaagaacaat atgaaaaagt
     3421 agatctaaac aaatatttcg ctgtttttac aacgattccg ttaaaaaatg taaatgaaga
     3481 gacaccggtg atccatttga ctaatctttt tgatgactct tggttaaggg cacaatggca
     3541 aaaagcaaaa aaaaagcgat tagccgggtt tcattctact gtttttttct atcaaatatg
     3601 gaaagaaaaa aagacgtatc aagaatattt aaacaaatta gtcgctcctt tgatcagagg
     3661 ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac
     3721 atttgttaac aaagaatttg cttttcctca cacaaccaac cagctatctc aacacattgt
     3781 tttatcagta gggctaataa ctgatcaagg tttagcagaa acaggaggaa atggagttat
     3841 ttttttattt gccatacctg aagaaatgac ggctgaagtt gagaatgaat tatttgagct
     3901 atacgatatc ctatttgaac tcataaaaat gggaatagat gagtttattg aattagcacc
     3961 gacacaagaa tcatttttaa aactaatcaa aagtaaagga gagagacttc atgaatgata
     4021 tgtatctatt catagttttc gcagcttcag cttatgtgct gcaagcagtt atcagttgga
     4081 agcaactaaa gcatttcaat caaacatata gcgaactgcg aagaaaagga aaagtagcga
     4141 ttggtaaacg acctgggaaa atccgttcag gcacgattgt tatgctagct atagacgatc
     4201 atgatcaagt tttagaagtc agaaaaatgc aaggggttac atctttggca aaatttcatt
     4261 cattacctgt ctatgcgggt gaagatatcc attacatcga tcgttaccat ccctcagttc
     4321 gagcggaaga tcgtttgaca caagcagctt tagaaaatgc cagagaagta tacttacgaa
     4381 tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc ttttattgct atgctcaata
     4441 attttcggat atggaaaagt acgatcaaag aaaaaaggag tgacaaacga tggatatcat
     4501 aacaaaattt gctgagggat ttatgaggct gttccaaaca ggtgcagaaa cattcataag
     4561 ctggatgaca ggaatcgtac cagttgtctt gatgctgatg gttgcaatga atacgctgat
     4621 tgctttttta ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga aaaatccgct
     4681 tactcgttac cttgtgttac cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt
     4741 tacaatggct cgtttccttc cagaatacta caaaccaagc tattatgctg ctcaagctca
     4801 attttgtcat acgagcaatg gtgtattccc ccatattaat cctggagaat tgtttgtatg
     4861 gatggggatt gctcaaggga tcgaaacctt agggctaaat tcgatggagc tggctatccg
     4921 atacttgctt gtgggactgc tgatgaactt tattggtggt tggataactg attttactac
     4981 tggatttgta tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc tttcagttga
     5041 ttagagaaag gagagtttta aatgtatggt gatattaaga tcgtaagagg aaacggcggg
     5101 tatggaggtc ctttgattat taggccgaca aaagataaac acaagttcat ctatgtcaca
     5161 ggtggaggtg agaggccaat gatcgtagat aaaatagaag aactgacagg tatggagtct
     5221 atcaatgggt tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat cgttgactgt
     5281 ggagggactc ttagatgtgg aatttatcct aaaaaaggga taccaaccat caatatcgtt
     5341 cccacaggaa aaagtggccc attagcacaa tatatcactg aagatattta tgtttcagca
     5401 gtagggttag aacaaataca gccactaatg gaaactgatg ataacaaaga aacagtaaat
     5461 actgcatctc cagataaaaa gtacaaatat gatacttcta aaaagataac cgagcaaaaa
     5521 gcagagcagc aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca
     5581 ttcaatcaat cagcaagaga agcagtagat acgatgatcc ggacagtgat cccttttatg
     5641 gctttcgttt cccttttgat tggtgtgata caaggttctg ggatcggtaa tgtatttgct
     5701 aacattatgg cacctttagc aggaaatgca gttggattaa caatcattgg ctttatttgt
     5761 tcactgccat ttttatctcc tttgcttggt ccaggggcag ttattagcca agtgattgga
     5821 acattgatcg gtgtagagat tgggaaagga aatattcctc cccaattagc attaccggca
     5881 ctatttgcta tcaatactca aaatgcatgt gattttattc cagtcggtct aggtttggaa
     5941 gaagcaaaag ctgagacagt agaagtaggt gtaccatcag ttctttattc aagatttctt
     6001 aacggcatcc cacgagttat cattgcttgg gtagcaagtt ttggattata tagctgagtt
     6061 tagtctaatg gtgataagtc aagatgaaat ttaagatttt ctttattgag taatatgttt
     6121 tgaagcgcac gaaactagac ccgatcaaaa tcaaattttt ttcgggttga ttcaaggatt
     6181 tgcgtcaata acggattgtt atttctctat cacactcctt ggtggcgtat agagttggtg
     6241 tttacgtagt agcgtatcca ccagacgcgt aaattttctt gcggttagga cgagtgcacg
     6301 tttgtgttga tgctttggaa cctccttata tttactttga taaaaagctt tgtattcagg
     6361 aagataattt tttacagagt tggcagcttc aactaagtag tatctaaaat atcgatttcc
     6421 ttgttttgtc agaggtgtat tttgcgactg atagtttcca gattggttca ctttccaact
     6481 taatccagcg tattttgcca attttgtctg atcttcaaat ctttcgattt gaccaatttc
     6541 agcgattaaa ccggcagcat agactttacc aacgcctgga atactggtta agcattgata
     6601 ttctggaatg acaacaatta cttgttcgat ggctttatct aattctttga tatttttttc
     6661 aagtgctcgt atttctcgta cgagaacact taaaacaaca ttaattgatt cttgtgcaag
     6721 agcacctagg cggtaactca tggtaattgc acgttgaatt gctttagcga ttttttctgg
     6781 ttgcttgaaa cggccttttc ctttttcttg aagtaaatca cagaaagctt ctaaaggaag
     6841 ctcagcaagt tcatccaatg tgaaatcttc cgtcatgagt gaaatgaggg ttgcactaaa
     6901 aagactcgtc gattcgtctc gcatttctcg agcaagcgta ttacatttgt acgttagatt
     6961 ttcaagaaaa tgttgctttg tacgaatcaa ttgcttaata agttgatagc gagtacgtgt
     7021 taaacgctgt aaggccatgt atttctcttc tttgataggg gaagttgtga atcgttgaat
     7081 acgcaagaaa tcagcgattc ttaatgcatc gtttcgatca gttttattct catcaaacat
     7141 gcgactaaat tgtttcaccc gaaaaggatt ttcaatcgtt acaagtgtgt tgagcttctt
     7201 tagtttctca tcttcatgaa aaaacatgga aggatggaag ctgtacatgg acgttgactc
     7261 catgccaatc acaa
//
LOCUS       DAPCMU010000127         7003 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPCMU010000127 DAPCMU010000000
VERSION     DAPCMU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.131"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            177..1034
                     /locus_tag="RD744_002248"
     CDS             177..1034
                     /locus_tag="RD744_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDU0558231.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1027..1929
                     /locus_tag="RD744_002249"
     CDS             1027..1929
                     /locus_tag="RD744_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDU0558232.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1962..2312
                     /locus_tag="RD744_002250"
     CDS             1962..2312
                     /locus_tag="RD744_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0558233.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2361..3283
                     /locus_tag="RD744_002251"
                     /pseudo
     CDS             2361..3283
                     /locus_tag="RD744_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            3361..4254
                     /locus_tag="RD744_002252"
     CDS             3361..4254
                     /locus_tag="RD744_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0558234.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4292..4855
                     /gene="amaP"
                     /locus_tag="RD744_002253"
     CDS             4292..4855
                     /gene="amaP"
                     /locus_tag="RD744_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDU0558235.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4869..5057
                     /locus_tag="RD744_002254"
     CDS             4869..5057
                     /locus_tag="RD744_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDU0558236.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5070..5972
                     /locus_tag="RD744_002255"
     CDS             5070..5972
                     /locus_tag="RD744_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0558237.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6004..6246
                     /locus_tag="RD744_002256"
     CDS             6004..6246
                     /locus_tag="RD744_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0558238.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6502..6873)
                     /locus_tag="RD744_002257"
     CDS             complement(6502..6873)
                     /locus_tag="RD744_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558239.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2553 a         1189 c         1391 g         1870 t
ORIGIN      
        1 acctttgaat aaaaagccat gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat
       61 aaaaaaattt taaaatattc aatgaaaaca aagcatatat tttgctttaa aaccaaatga
      121 ggaatattct agatgtagaa caggttagaa aaagattaga aaaaggagtg attttgatgg
      181 agaagagata cggcgtagtt cctgtggtaa ctgcttcaga cgaaaattat gctccttact
      241 tgagcgtgat gatcgcaact gcattagaaa attgcaataa aacaagacgc ataaaatttt
      301 atgtgattga cgatggtcta tcagaatata gtaaagaagg attggaagaa acggtaaata
      361 aatattcaag taatgcaagt atccaattcc tgacagtgga aaaagatatc tacgaagact
      421 tcctagtcag tgaccatatc acaactaccg cttacttaag aatttcttta ccaaacttat
      481 tagctaaaga ggattataag aaagtccttt acttagattc agatgtgtta gtactagacg
      541 atatcgtaaa actatatgat gagccgttaa atggaaaaac aatcggcgct attatcgatc
      601 caggccaagt gaaagctttg gaaagattag gaatcgattc cgatgacttg tacttcaatt
      661 caggagtgat ggtgattgac attgatcaat ggaataaaaa agaaattaca gaaaaaacta
      721 tccattacct cagtgaaaat ggcgatcgaa tcatctatca cgaccaagat gctttgaatg
      781 ctgtacttta tgaagattgg gaacaacttc atccaaaatg gaacatgcag acttcgctga
      841 tttttgaacg tcatccagca ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg
      901 aaaaaccttc gatcgttcac tttacaggac atgataaacc ttggaatacg ttgaaagatc
      961 atccgtatac caatttatac ttaaaaaaat tagcacacag tactctaacg aaagtaggcg
     1021 agatcaatga ataaaaaaga aatagcagta gtcgcaagtt gtaacacaaa attcgtacca
     1081 catttagctg cattatttgt ctcagtatta gataattgca acccgtctaa atttgttcgg
     1141 ttctatgtga ttgacgatga tatcgatttt gaaagtaaac agcttttacg cttctcagtg
     1201 aaaaacgcac gaatgaactc agatgtggaa ttcttgaaaa tcaataaaga attctttaca
     1261 aatgtggtaa tcagcgatcg tattccagaa acagcttatt atcgtattgc gattccggaa
     1321 ttgttccgcg gcacagaagt tgaacgtatc ttatatatgg attgcgacat gatcgcttta
     1381 caagatatct ctaaattatg gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa
     1441 gacgctgggt tccatcaacg actggaaaaa atgaaaattt cagctaaatc aatgcgttat
     1501 ttcaactcag gtttgatgct gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa
     1561 aaagtattag atttcatcga acataatcca gaaaaattac gattccatga ccaagatgca
     1621 ttaaatgcta tcttacatga ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc
     1681 tatatcatgg caaaagcaaa aaaacatcca acagcagcag gtgaacgtga atacgaagaa
     1741 actcgcaaca atccttacat tatccatttt tcaggacatg tgaaaccgtg gagcaaggat
     1801 tttgaagggc caacgaagaa atattacgaa aaatatgcag ggatgacggc tttccgttgt
     1861 gtagctaaat tccctaaata tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt
     1921 gttaaataat agaagacaac aactgaagag ggggagtagg aatggaaatt acaatgtcta
     1981 aaaatgcagt agagacgtta attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc
     2041 ttatcaatga ttccgatcca tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt
     2101 ttcaaatcat tccttttgtt tcggaatttg gagaatatgc gactaaaatc gagcatccat
     2161 tattggacat ctatacatct aaattagagc aaaattattt cggtaaacga ttgaacatgg
     2221 acttcaacaa acagcttgac agcttttcat tagaaaatga aatcgcagta ctcgattata
     2281 atatcaaatt aaagaactgc ttcttcagct aatagtttcg agaaagtcca aaaaggaggt
     2341 atacattgag atactacttt gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa
     2401 aatataattg gctcgtactt gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag
     2461 aattcgagct tcctgatgat attttcgtag gaaccgaata tccggaagaa gtttctcgga
     2521 tggagcgcct ttatgagacg aaactagagc atccattttc tttggtagtg attaatctag
     2581 atcagtcaga taatcgtatc gaaaaaaatt aacgccaata tcttttgtac tttccgataa
     2641 tctcttgatt acttgtatcg atagaaaaac cgaatttgtg gatcggctgc tgacaaagca
     2701 tggaaatcat ttaaactcgt ttgaaaagat catcacgtat acattattag atatttacac
     2761 ccattacgtg aaagaactaa gagagatgaa aaagcgaatt gacgctttgg ataaagcagc
     2821 tcgtaaaaca acggaaaatg aagaactgta taaacaggca gatcttgaaa gagacatcgt
     2881 atatatcgat cataccttaa gagatcaacg ggaaacaatg gatcaattat ggaattctga
     2941 aactttcatc aaacgtttag acgatgaaca gctactctat gatgtccatt tgagacagcg
     3001 acaaacagaa aaaatgatcg agatctatcg agatttatta gagagtattg gtgatctatt
     3061 caacgggatg atggacaata atctaaatca tatcatgaaa tatctagatt cagctgcatt
     3121 aatcatttcc gttccggcgt tgttcgcagg gatttgggga atgaatacag gaggattacc
     3181 gggtaaaagt tctgctctag gctttatact cgtagtcttt ggctctttga tagcagcagt
     3241 agttttaggt taccatttat accggaaaga ttatacaaat taaagaatct atttaaaaca
     3301 ggtatcaaac aaagaaattt gttttggcaa tacaatttaa aaactaggag gaatacagca
     3361 atgacagaac caaaattaat cgacccgcga aacttgtacc atacggagaa atttcctaaa
     3421 caagatcagg atacaccagc tttacagcag aatatgattc ctgttcctga ttgtggagaa
     3481 gaatcctatg aaggcaataa tcaactagaa aaccgccgtg tactaatcac tggtggagat
     3541 tcagggatcg gtcgtgcagc agcgattgca tttgctagag aaggggcaga tatcgctctt
     3601 caattcttcc caggagaaga aaaagatgca gaggaagtag caaaatacgt gaaagttgca
     3661 ggacgtaaga ttgtcttact accttttgat cttcgtgacg aacaagcacc taaagaaatc
     3721 atagataaag ccgtgagtga acttggcggg ctagatacga tggtactaaa cgcaggacag
     3781 caaatttctt gtcagtctat tgctgagtta ccacttgagc aagtcaaaga cacatttatg
     3841 ataaacatca tcgctatgtt tgctttagtg aaagaagctg ttccgcatat cccagcagga
     3901 ggagcaatcg taacaacaac ttctgttcaa gcattcaatc caagcgaaca tttattagat
     3961 tacgcagcaa caaaagcttc aatcgctaac tttacgatcg gtttagcaaa acagcttgca
     4021 cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa tctggacacc attacaacta
     4081 gacgaaggac aaccttctga ttcgatccct gaatttggtc agcaaagttt actagaacgt
     4141 gcaggtcagc caggagaatt agcaccagtt tatgttttct tagcatcgaa taaagcaagt
     4201 tatgttacag cccaaattta cggagtaact ggtggagaag cgatcaactt ataagaagtt
     4261 gatcgtttca cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt
     4321 gatcggaaca ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta
     4381 tccattgcct tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca
     4441 acaatatgta ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt
     4501 tttcctgttt tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt
     4561 gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt
     4621 catccattca ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa
     4681 aggggagttg aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat
     4741 cgaaaatgaa gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata
     4801 cacaggcttt gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg
     4861 gaccaaaaat gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg
     4921 gattgatatt agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga
     4981 tcatggcagc gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc
     5041 actattttaa atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata
     5101 aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa
     5161 atggtgtgaa cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag
     5221 tccctaacgt tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc
     5281 atcatccagg aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta
     5341 aacctgtgga agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt
     5401 ctgaccacac aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc
     5461 atgattctga acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc
     5521 aaaaaatcat cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt
     5581 tcttctctaa tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa
     5641 cagaagtcgg taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag
     5701 atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga
     5761 cacatttaga agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat
     5821 ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg
     5881 aatttgcttc gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag
     5941 taaaagaaaa tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga
     6001 aacatgggat ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca
     6061 atcacgaata aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga
     6121 tcagcacttg gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc
     6181 gtgccatcat tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa
     6241 ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa
     6301 cagccaaaca aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc
     6361 aagcttttga acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct
     6421 cttatttatg cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt
     6481 tattgataca gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg
     6541 aaaggtgaac atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat
     6601 cgtatgatac tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac
     6661 aaggaagaaa taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa
     6721 gtaatctaga caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg
     6781 ttgctctgct agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag
     6841 taatgaaaga aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg
     6901 ccctcatttt aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta
     6961 tctaatgtat ttttaaataa ataatcatta ttatcaaaat aat
//
LOCUS       DAPCMU010000128         6911 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPCMU010000128 DAPCMU010000000
VERSION     DAPCMU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.132"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..368)
                     /locus_tag="RD744_002258"
     CDS             complement(<1..368)
                     /locus_tag="RD744_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDU0558240.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTL"
     gene            complement(555..1205)
                     /locus_tag="RD744_002259"
     CDS             complement(555..1205)
                     /locus_tag="RD744_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558241.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1748..2683)
                     /gene="whiA"
                     /locus_tag="RD744_002260"
     CDS             complement(1748..2683)
                     /gene="whiA"
                     /locus_tag="RD744_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDU0558242.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2717..3715)
                     /locus_tag="RD744_002261"
     CDS             complement(2717..3715)
                     /locus_tag="RD744_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDU0558243.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3712..4596)
                     /gene="rapZ"
                     /locus_tag="RD744_002262"
     CDS             complement(3712..4596)
                     /gene="rapZ"
                     /locus_tag="RD744_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDU0558244.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4731..5462)
                     /locus_tag="RD744_002263"
     CDS             complement(4731..5462)
                     /locus_tag="RD744_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDU0558245.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5466..6731)
                     /locus_tag="RD744_002264"
     CDS             complement(5466..6731)
                     /locus_tag="RD744_002264"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDU0558246.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         2063 a         1474 c         1082 g         2292 t
ORIGIN      
        1 cttaaagtaa caaataaata acgtattcca atagaaaaat cactataaat actctctagc
       61 ataggaccga caattgaaga agtcactact gcaaatgctg gacctatcca agcaacatta
      121 gaatgttctt tctctctttt cctttgtaaa tctttgttga gtattaatct atttatttct
      181 tctgcaggca agttaatttt cataacctta tgggggatta tccaattaaa aatcggaaaa
      241 aggtagcctc cccaaaaagt atagtgatct gtgtcaatca gataataacc tgattttgat
      301 tccagaataa catatctcaa agccgaacga aaatttttca tgaatatcca taatctcact
      361 gtttccacac ctaattcctc caattccaat taaatggatt tatagcatca ccaattatgt
      421 taactgcctg accaacctca ttgataaaat tggcaccact atcccatgct tcaacaatgc
      481 tatatatcta tcaaacaaaa aatggaaaac taaatactcg cgcacataaa ttttacattc
      541 tgttttttat aagactaata ataatcattg aaaaaatttc ttatcggtgg cgcatattcc
      601 caattgatta atgtgatccc atattctgca agtattttac gagcttcttc taagtcatag
      661 tgagaaaaat gcataaaacc tgcaatacga tcacggactg tgagctgtgg cttgcttcca
      721 ccatcattca taaaatatcc agcagagcca ctacttaaag tgattgttac atcttcactt
      781 tctattatta agaattcata atgaaatgct ttcaactctc cactattatt taaaagtttt
      841 atactccaac ttcgtctagg ttgagttgtc acattccctc taaaacttcc tataaattct
      901 gcaatcaccg tatcttcaat caaaattctt tcctcatcta tttcatattc aagactaaac
      961 ggaaattctc catatgtaat ctctggcaat ggtggtgctg tagttctcca aataaataca
     1021 ataactgtga atagaaaaaa tccaacgatc gatataaatg ttatgagacc tgctcgaagt
     1081 aaaacccgat ataataaaaa tgctgtattt cgctttgcta tatctagaaa aataaaaagt
     1141 actgcaatca taatgatagt aaaagttgta aaaaagcaca ttttttgccc tccctttata
     1201 aacatcgatt ctcaaattct aaaccaaatc ttgatagttc aagttcaggt ataacgtata
     1261 ccaaccatac atcccaatta ctatttatga acttatgatt atcactgaat aaatcactcc
     1321 atccccttgt taaaacttct acattaatta cacctggttg ttttcgacta gctaatccaa
     1381 tgaacttatc aaccatcctt atccaccaac cacgagaatc ttgaccagaa gtaagaaaaa
     1441 cagttctcaa ggttgatcga gcagttcctt ccagttttta attaatctcc agttatagat
     1501 aaatacttga tcctatctat atcaggactt ctatattaag caaaagttta tgtataaaac
     1561 taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa aacaatagaa
     1621 gcctttcaac attatgttac tccagtaaag ttggaattat agtttgacgg aagaaaagca
     1681 agtatagctt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
     1741 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
     1801 ggtgattaat tcccgatttt gatattgcac ctgagggaat catttcccct aattctttca
     1861 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
     1921 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
     1981 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
     2041 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
     2101 ttaagaaatc agcaattttt tctgctcctt ttaaatacga aatatagcca ttgcgccgtt
     2161 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
     2221 gttcataaat agaaaatatt tccaaatgat aacgactcgt ttccggatta ttgactgaac
     2281 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
     2341 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
     2401 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
     2461 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
     2521 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
     2581 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
     2641 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
     2701 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
     2761 ctttgtctcc atcgtggaag acaccaccat ctttcaattc taaaaagtcg gtggaaatca
     2821 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
     2881 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
     2941 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
     3001 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
     3061 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
     3121 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
     3181 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
     3241 tgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaaaaca gcatgcaagg
     3301 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
     3361 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
     3421 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
     3481 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
     3541 ccatagcgat cgattctctg attgcaccgc tgcttccacc atcatctgca acagtcacta
     3601 ctgctgtgat atctgctcct tgatttcgta aacttttcag gataactggc agaccagtgc
     3661 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
     3721 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
     3781 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
     3841 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
     3901 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
     3961 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
     4021 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
     4081 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
     4141 ttcacgtaat tgtcgtggtg tcaatgttgt cgtatcaatg atcacggaag cttgggcttt
     4201 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
     4261 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
     4321 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
     4381 gatttcttca aagaaggaac gagaacgcaa atccactcct aaagccattt ttgtgacttt
     4441 acctgattct ttgatcaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
     4501 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccactcat
     4561 acctgttatg atgactaatt gcaaattatc tgtcatattt acgaacccct ctcacattgt
     4621 gcataataaa attatatcat cccgggcgta tcattaccag ttttcttttt cttttacaaa
     4681 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
     4741 gatgctgtat ccaatgcctc gcttctttca gctaatgcac gttcgatcgt tcgatccgct
     4801 aaaatagatt gagcatcgat cactgggtaa tgattatctt cagcaaattc atgcatcaaa
     4861 gataattctg tgcaacctag gatgactttt tcacagttta ggcgctctac cgcttcctct
     4921 aagatttcgt aatagagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
     4981 aggtaattga ttttttcttg aagcgcagta tcaggaatca ctgtttcgaa accaagattt
     5041 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
     5101 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgcaagata
     5161 ggaatatccg ttgccgcttg taactcttca aaaaagtagt gtgcggtgtt acaagtcaac
     5221 acaatgaagt ttggtcgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
     5281 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
     5341 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
     5401 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
     5461 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
     5521 atatatttca tcaacaacca gcgcttgatt gatcggtctt tttcataaaa tacagtagta
     5581 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
     5641 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
     5701 gttccatagg tcgtttcaac aaagggtttg ttgaacactc gatcttctgt gacaaaacga
     5761 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
     5821 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
     5881 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
     5941 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa tagtggatga
     6001 ccaaggcaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
     6061 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
     6121 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
     6181 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
     6241 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
     6301 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
     6361 atcagccttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
     6421 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
     6481 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaatacaggg
     6541 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
     6601 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
     6661 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
     6721 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
     6781 ggaatatcat accataaaga aaaaagcaat caaaggaaag tcctccgatt gctcaattct
     6841 tttttgaacg gattgaactt ataggaagtt tttatctata ccaatcagtg tagaaagaac
     6901 taccatgtaa t
//
LOCUS       DAPCMU010000129         6896 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPCMU010000129 DAPCMU010000000
VERSION     DAPCMU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.133"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..182)
                     /locus_tag="RD744_002265"
     CDS             complement(<1..182)
                     /locus_tag="RD744_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0558247.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="RD744_002266"
     CDS             517..951
                     /locus_tag="RD744_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDU0558248.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="RD744_002267"
     CDS             974..1294
                     /locus_tag="RD744_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558249.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="RD744_002268"
     CDS             1435..1626
                     /locus_tag="RD744_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558250.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="RD744_002269"
     CDS             complement(1697..1954)
                     /locus_tag="RD744_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDU0558251.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="RD744_002270"
     CDS             2112..2375
                     /locus_tag="RD744_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558252.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="RD744_002271"
     CDS             2399..2587
                     /locus_tag="RD744_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558253.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="RD744_002272"
     CDS             complement(2670..2855)
                     /locus_tag="RD744_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558254.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="RD744_002273"
     CDS             3070..3441
                     /locus_tag="RD744_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDU0558255.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="RD744_002274"
     tRNA            complement(3591..3663)
                     /locus_tag="RD744_002274"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="RD744_002275"
     CDS             complement(3709..4137)
                     /locus_tag="RD744_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558256.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="RD744_002276"
     CDS             complement(4197..4955)
                     /locus_tag="RD744_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDU0558257.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="RD744_002277"
     CDS             5250..5447
                     /locus_tag="RD744_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558258.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="RD744_002278"
     CDS             5553..6035
                     /locus_tag="RD744_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558259.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="RD744_002279"
     CDS             complement(6093..6689)
                     /locus_tag="RD744_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDU0558260.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2333 a         1060 c         1355 g         2148 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttga
//
LOCUS       DAPCMU010000130         6851 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPCMU010000130 DAPCMU010000000
VERSION     DAPCMU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.134"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            21..1175
                     /locus_tag="RD744_002280"
     CDS             21..1175
                     /locus_tag="RD744_002280"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDU0558261.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1197..1949)
                     /locus_tag="RD744_002281"
     CDS             complement(1197..1949)
                     /locus_tag="RD744_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0558262.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2313..4979)
                     /locus_tag="RD744_002282"
     CDS             complement(2313..4979)
                     /locus_tag="RD744_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDU0558263.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5320..6771
                     /gene="cls"
                     /locus_tag="RD744_002283"
     CDS             5320..6771
                     /gene="cls"
                     /locus_tag="RD744_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDU0558264.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2023 a         1412 c         1248 g         2168 t
ORIGIN      
        1 ataagtaaag aggtgttgaa atgaaaatag ggattgatcg tctttccttt tttattccta
       61 atttatattt agatatgaca gagttggcag aaagccgtgg ggatgatcct gcaaaatacc
      121 atatagggat tggtcaagac caaatggcag tcaatcgtgc aaatgaagac atcattacac
      181 taggagcaaa tgctgccagc aaaatcgtaa cagaaaaaga ccgtgagcta atcgacatgg
      241 tcatcgtcgg aacagaatcc gggattgatc attcaaaagc gagtgcggta attatccacc
      301 atttactgaa aatccaatct tttgctcgtt cttttgaagt aaaagaggct tgctacggtg
      361 gcaccgcagc tttgcacatg gcgaaagaat atgtcaaaaa tcatccagaa cgaaaagtac
      421 tagtcatagc aagtgatatt gctcgttacg gcttggcaag cggtggtgaa gtgacgcaag
      481 gtgtcggtgc tgttgcgatg atgatcactc aaaacccgcg tattttatcg attgaagacg
      541 acagcgtatt tctgacagaa gatatctatg atttctggcg tccagattat agcgaatttc
      601 ctgttgttga tggtccttta tctaattcta cgtatatcga atcattccaa aaagtttgga
      661 atcgacataa agaattgtcg ggtcgaggac tcgaagatta tcaggcgatt gctttccata
      721 ttccgtatac taagatggga aaaaaggcat tgcaaagcgt attagaccaa acagacgaag
      781 aaaatcagga acgtcttatg gctcgctatg aagaaagcat ccgttacagc cgacgaatcg
      841 gtaatcttta cactggttca ttatacctgg ggctaacttc tctactggaa aattcgaaat
      901 cactacagcc aggagatcgc atcggtctat tcagctacgg ttctggtgca gtaagtgaat
      961 tttttacagg ttatctggaa gaaaactatc aagaatatct cttcgcacaa tctcatcaag
     1021 agatgctaga ttctcgaaca cggatcactg ttgacgaata tgaaacgatt ttcagtgaga
     1081 cgcttcctga acacggagaa tgtgcagaat atacttcaga tgtacctttt tcgattacaa
     1141 agatcgaaaa tgatattcgt tactacaaaa tataataaaa tcataaaaag aacccttcac
     1201 gagtcgtgaa aggttctttt tttatctgcg gaccaaagga gcagtctata ggtcagctta
     1261 gtcgtactaa ggatttctcg tttttcactg aatagtttgc gaatgtacct ggtcacaaca
     1321 ggatcttttg cgtacgcgtc atagtatagc ttcatcaatt cttcagtaat tgattcctgt
     1381 gtaaaataag taaaatcttt tttcttcact ttgaatcctt catcaattaa gtatgtttca
     1441 aattgatcca ttacaactgg cgatgtatta ggatcttctt ctatcattcc tagttgcttt
     1501 tcgataggat aaaaaacatc atcgattgat tccactactc tgattatcac gcaccatttg
     1561 cttgactttt tcatcaattt ttctaattga ataagatgta ccaatgctgg atttgctgct
     1621 gtaaaaacca tttcatatgt ctcatttgat tggataaatt cttctaacga ctgtttgata
     1681 aaggaaacat ttggcaactc atttttttct gcttcttttt ttgcattttc tagcattcct
     1741 gaagaaaaat cgatcaaatc atagcgatta atttcttttg ctaaggaaag aaatctgcca
     1801 gatcctccag caacgtctgc tgctgtttca gcaggcagga ctttctccgt tttcagataa
     1861 ttttttacat cttcagcaat agttacttgc gattcttgct ggatttcata atattcttca
     1921 gcaaattcat cccattcttt ttcattcatt tgatttcctc taattttttg ttatatgttt
     1981 tcacttttcg gaataaatca ttctcctctt ttatctattc tatctctttt gatgaataat
     2041 aaaatgattg gctttccgtc tatcataaat aaaaaaaggg actgtgacca aagtcgtgtc
     2101 aaagtccctc tttccgaata aacagtgtcc aatcagatga ccctcagtat tagctcaaaa
     2161 tgctgatttt acaacttctt ccttaaacgg tgttctcctg ccaactcttc ggtcacaagt
     2221 cacaatattc aacaaacatg agaagctgtt tgttgaaatt gcaccttgtg actcagaatt
     2281 aaacggcagg ctcacaacct cgttgattcg ttttatttat ttccttgtac tcgtacaatg
     2341 atcaatttag tgatttccat caaaacaatt gtcacaagtg cgattcccat acatgtaaag
     2401 acttctgtca atccaaatgc tgatgggata gagaagactt ctttcagtcc tgggatcatt
     2461 gttaagcagt aaagcgcgct acatacaact acagctagta agacacttct gttagagaag
     2521 aaccctgctt ggatagctgt ttgtgtgttt gaacgtgctg ggaatgtttg caatgtccga
     2581 caccaaatca atgttgaaaa tgacatggct acaccgaatt caggtgaatg ctgcatgcca
     2641 agccattgag caaagataac agcaatccca atcagcacac cacgatacgt tacagaaatc
     2701 aatgttttgt ccgtaaagat tcctgaattt ggatctcttg gcgggcggtt catcgtagca
     2761 ggttcagctt tttctgtacc taatgcaatt gctggcagtg aatcattgac taaattgatg
     2821 aacaacagtt gtaatgctgt aaatggattg gaccagtcag caactaaggc aaagatgatc
     2881 gcaatgatcg cacctaaatt tcctgcaaat agataagaaa tagctttctt gatattatca
     2941 aacacattac gtcctacttc tactgcgctg acaatagaag caaagttatc atccgtcaat
     3001 accatcgctg ctgcatcttt tgccacatct gtacccgttc ccatagcaat tccgatgtct
     3061 gcttgtttca atgctggtgc gtcatttaca ccatcacctg tcatagctga gatatgacct
     3121 ttattttgcc aagcacgaac gatacgaatt ttattctcag gtgaaacacg tgcataaaca
     3181 gtgacttttt ctaaaacttc gtttagttga tcttctgtca attcatctag ttccgtcccg
     3241 gtcaaagcca ggtttccttc ttcaaaaatc ccgatttgtt ttgcgatagc aacagctgtt
     3301 gttttgtggt caccagtaat catgattgtt ttgattcctg cacttcttgc ttctgttacc
     3361 gcctgagtca cttcagcacg tggaggatcg atcatagcaa gcaaaccaac aaggatcaaa
     3421 tcgtcttcat cttcaaatga taaatcaaaa ttatcaagtg gacggtaagc aaaagctagt
     3481 acacgtaatg cgcgtgttga aaaagcttca ttttgctctt ggaatttttt catcaattca
     3541 tccgtcatcg gaacgacttc accatttagc aatacttttt tactgcgttg gaagacgata
     3601 tctggtccgc ctttaacaaa caagtaattt ttgttatcaa ttgtgtgtcc agtagacatt
     3661 aattttcgat ctgagtcaaa aggaagttct gcttgtcttg ggtagttgtt tcggatttcc
     3721 tgataaggct ggtttaattt ttcactaaag tcgataaacg ctacttccgt agggtcgccc
     3781 aatttagtac cgtcttcact gatcgatgca tcattcgcta aaacactgat ttcgatcaag
     3841 cgacgttcgt cagctgtcca ttttgaaggg tcatttgtga aatccccagt atcgccatta
     3901 cctaaaaagt agtcaacaac tgtcattttg ttttgtgtca atgtaccagt cttatctgta
     3961 cagatgatac ttgtcgaacc aagtgtttca actgctggaa gcttccgaat aatggcatga
     4021 cgttttgcca ttttattcgt tcccaaagat aacacgattg ttacgataga ctgtaatgct
     4081 tctggaatag cagctacggc tacggctacg gcaaacatca ctgcgctgac gatatctgca
     4141 gtcatatctc ctgtaccttc tccaagataa atacgaaaca gttggatacc taaaatcaat
     4201 aaagacaatg ccaaaatagc caaacttagt tttttactga atttatccag acctcgttgt
     4261 aacggagtga cttgctcagt tgttgattcc aaaaggccag caacttgtcc gatctccgta
     4321 ttattcccag ttgaagtcac taagaattta ccgcgtccgt aagttacgat cgttccactg
     4381 aataccatat ttgtacgatc tccgattggt accgtacttg tgatatccgt tacttctttt
     4441 tctgcagaag tcgactctcc tgtcagcata ccttcatcta cttttaatga tcccgcatct
     4501 agtaaacgtc catcagcagg tacataatcg cctgcgtcta acaacacgat atctcctacc
     4561 acaagttcgt ttgccggaat cgttttttcc ttaccatcac gaatcactcg agcatcgggc
     4621 gctgaaaggt ttttcaacgc ttctaaagaa ccttctgctt tttttgtttg gacaacactg
     4681 attatagaat tcaacatcaa tactgcaaaa ataactaaga attcgatgta gtcacccatg
     4741 accatctgaa tcagtgcgac aatcagcaat acgataacca ttgcatcttt gaaagtttct
     4801 aagaacaatt tccaagttgg cttcttcggc ttttctttta gctggttcaa gccatctctt
     4861 tctaattttt ccttcgcttg cgtggtgctc aatccatcgg ttgagctctg cgtttgctgc
     4921 atcaacgagt cgatcgtttc tttatagacc gcttgttgtg cttgttcctt gttttccaaa
     4981 tttcatcccc cattttttga tcttggccct agtcctgtct gaaaaaaaag agacctaaga
     5041 caaatacgca tttgtcttag gtctcaccat ttcatacaat accagaaaac catttggtta
     5101 acttactgtt gatattatcg caacaaacgt tgccggttac tcccctaagg agatattcaa
     5161 ctgtactaat atcctacctt attaacctac aaaaagcaac tactttagcc cgatttacct
     5221 acattttcat gaaaggagtg aaaagtatat tcaccttggt aggcttcttt cttctatcat
     5281 gctatactaa tgttattgta tgtaaggagg aacatttttg tggtatctag tattataacc
     5341 gccctttatc tattaaatgc acttattgct ttggtggcta ttttgatcaa accccgagat
     5401 gtagcagcca tttgggcatg gctcttagta ttgtttgccc ttcctggggt gggctttgta
     5461 ttatatttat ttttcggacg tggattaacg gataagaaaa aattctatct ccgacaaagt
     5521 gacttgaaag aattagaaaa ctttcagtct tttagaggag ataccattga acattacgat
     5581 cctgacatgg gcgataaaga caaacagcaa tttgttgact tcttctcttc attaaatcgt
     5641 atgccgctga caagaatgaa ttctgtcact cttctcacag acggacaaga gaaattggat
     5701 tcactgcttc aagatctaaa aaaagccaaa cattcgatcc atatcgaata ttacgcattt
     5761 gtgacagata atatcggcca gcaagtctta catgttttag aagaaaaagc cgcagaaggc
     5821 gtggaagttc gaatattata tgatgcattt ggctctcatg gcacaaaagc aaaagatttc
     5881 aatcgtctaa tcaaaaatgg tggacatgtc catacatttg ttacctcaca aagggcatta
     5941 cttcgtttcc gattgaatta ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt
     6001 agttataccg gtggtttcaa tattgccaat caatatgtaa atacaacaaa aaaattcggc
     6061 tattggcgcg atacgcatat acggattttc ggtgccgctt cttctttgct ccagcttcgc
     6121 ttcttaacag actggaacgt ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat
     6181 tatttcttta aaaaagcaga tcgagatgaa tctaagcttg ctgatacatc catccagctt
     6241 gtttcaagcg gaccgaataa cgaaagggaa caaatcaagc tttcatttat caaattgatt
     6301 acttctgcta aaaaacgtgt ttggatacag acaccttacc ttgttcctga tgaaagtgtc
     6361 attgctgctt taaaaatcgc aactgcctct ggtgtagatg tgaaaatcat gattcccaac
     6421 aaaccggatc atccttttat ttatcgagca acacaatatt atgctcggca gctgatcaag
     6481 gaaaatgtac aaatccttgt ctatgagaac ggcttcctcc atgcaaaaac attgataatg
     6541 gatgatgaaa tctgcatggt aggttcagca aatcaagata ttcgtagcta ccgattgaat
     6601 tttgaaacaa gtgctgtcat ttacgatcct gagtttttag aagaacttgc tactcagttc
     6661 aaagaagatg agacacattg ttcatccatg acaactgaaa cagtcaagga aatgtctaac
     6721 tggctattat tcaagcaaca aatttctcga ttattttctc caatcctata atttttttat
     6781 tcaagtagag aaaaatatac taagattaaa gggactgtga caaaaatctt gtcacagtcc
     6841 cttcttccga a
//
LOCUS       DAPCMU010000131         6840 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPCMU010000131 DAPCMU010000000
VERSION     DAPCMU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.135"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(342..1241)
                     /locus_tag="RD744_002284"
     CDS             complement(342..1241)
                     /locus_tag="RD744_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDU0558265.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1262..1534)
                     /locus_tag="RD744_002285"
     CDS             complement(1262..1534)
                     /locus_tag="RD744_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HDU0558266.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            complement(1557..2963)
                     /gene="lacG"
                     /locus_tag="RD744_002286"
     CDS             complement(1557..2963)
                     /gene="lacG"
                     /locus_tag="RD744_002286"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDU0558267.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3105..4811)
                     /locus_tag="RD744_002287"
     CDS             complement(3105..4811)
                     /locus_tag="RD744_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0558268.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4811..5128)
                     /locus_tag="RD744_002288"
     CDS             complement(4811..5128)
                     /locus_tag="RD744_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDU0558269.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5156..6136)
                     /gene="lacD"
                     /locus_tag="RD744_002289"
     CDS             complement(5156..6136)
                     /gene="lacD"
                     /locus_tag="RD744_002289"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDU0558270.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            complement(6139..>6840)
                     /gene="lacC"
                     /locus_tag="RD744_002290"
     CDS             complement(6139..>6840)
                     /gene="lacC"
                     /locus_tag="RD744_002290"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDU0558271.1"
                     /translation="PAFYKISGNTRNCIAILHEGNQTEILEQGPTISHEEAEGFLDHY
                     SNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAVVLDCSGAPLETVLKSSAKP
                     TAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEWIVVSLGRNGAFAKHGDVFY
                     KVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKHAMTLGMLNAQETMTGHVNM
                     TNYETLNSQIGVKEV"
BASE COUNT         2117 a         1391 c         1214 g         2118 t
ORIGIN      
        1 acgctatatt taagatttca aacaactata ttaataggaa gaaaatttca tttttcctct
       61 tagttctata aaaagtgtgg ctaatttgag gcaaaatatt aaattaaatt tgccacaaat
      121 taatggctcc actattattt taaatcaaga agtagtaaat aatcttattt tttactacca
      181 gactgtcttt agatattagc ttttcgtcac ccactacagt tgacatagac ccacaaaaaa
      241 cttaaaaacc ttgcgatgta agactggcac ggagaagacc aatctatcat cacaaggtaa
      301 gagtattcaa atcttattga agactcaaat aataacaaat tttaattaag gatagtaata
      361 tcatagattt ttctagaagt ttcttgaggt gaaattttcg ttacctgtct tttatcttct
      421 aagaaattac cttcttcaag tgaagtggag agaccactcc aaggttccaa agcaatgaat
      481 ggacttttat tagttgtaga ccacagaatc aagttcggaa aatcatcaaa atctactcga
      541 agacctttac cagacttcct tgaccgaagc gttacgctac gagacttgag ctggtcaagt
      601 gtaatggcat catgggaaaa aagtgagtag tccaaatcga gaattttttg attttccaaa
      661 aaaggtgtcc gatcttgcaa atctaataat cctgtctcag gaaaactctt tggtatcgag
      721 caagactcaa cctcactaaa ttcaatataa taatcctcat agttttcacc ttctactaaa
      781 ggactattga atgctggatg ggctccaata aagtaaggca ttgttttttc catttccaag
      841 ttcgttacct gaaattcggt tctaattgat ttaccattca aggtataaac aactctcagt
      901 tcaaactgat ataggtaatt atcaagcatc tcagcatttg gtttaatact aaaggtcacg
      961 ctattatcat tgacttcttc cagaataaat tcttcttttc gaacaaaacc atgtctacga
     1021 ataagacctg taaaaaaggg tctttcttga ggtctataaa tagcccaatc atttcgtaaa
     1081 ctaccacaga taggaaatag aataggcgcc tgtccattcc aataatttgg atcagcttgc
     1141 cagagatact ctatcccatc cttgtcttta atcgaagtca gttgaccacc aagtgtttta
     1201 aactgaacag taagatactc gttttttaat tcgatagtca caactaattc ctttcttttt
     1261 attattggaa aggcaaataa tttacctcga agtgaatagt ctcacgtatt tctacatcga
     1321 gtgattgtaa aaattcccat tcaaatgctg ataactctaa atcagaatgg caaatccact
     1381 cttttccagt tgaaacatac catttaccac attttagcaa cggggtagct tcaagctcag
     1441 gaatcatagg acaatcttcg aaactaacaa tatatacctt ttcaaagcct ggtctaaata
     1501 agtgttgtac tttttctaaa gtatcggata acaatttttt acattttcaa gtaatattac
     1561 tctatcactt gagtttctgc tactttctta taccaatgag ctgatttctt aggatagcgt
     1621 tcttgcgtgt caaagtctac atagaatagt ccataacgtt tttcgtatcc gtttgaccat
     1681 gagaagacat ccataagtga ccagatgaag taacctttaa catttgcacc atccgcaatt
     1741 gcgtctgata aaacttccaa gtgtttcttc acgtaatcaa tacgaccatc atcataaacg
     1801 gtattatcta caaactcatc tttgtatcca agaccgtttt cagtgatgta aatcttcttg
     1861 tagtttggat aatcattttt cactcgcatg atttggtcat acaagccttc aggataaata
     1921 atccagtccc aatccgttct aggcacataa tctggtgcga ttcggcgacc aacaccttta
     1981 atctggtact tagaacttcc tttttcaccc ttaccattgt gaatgatttc agtctcacca
     2041 tcaaaggctt gcatccaatc actcatgtag tagttgatac caaggaaatc gttcaaatct
     2101 ttagctgctt caagtgcttg gaagtcttca tcacgaagat caagttctcc accattttca
     2161 gctaggatgt ggttgacccc ttccatcgtt ttatctgaat agtgtccaag gtaggttgca
     2221 tccaagataa atttgttatg gatgatatct tctaactcag cggcacgaac atctgctgga
     2281 ttttctggat cataagggta tttggttggc aacgcatgta ctacaccaat ttcgccttta
     2341 taacctttat ctttatacaa tttcacagca cgtgcatgag aaaccatcat attgtggtgt
     2401 gattggaaga ctttggcaag atcatattga atgcctggag ggaatttacc aaccaagtat
     2461 tgaccatctc caattgggcc aatttcattg aaagttgtcc agtagtttac ttctgggaac
     2521 tcttcaaaac agaaagcggc ataatctaca aagtgttcta tattttcgcg gtttaagaaa
     2581 tctccatctg agtgaagaac ttctggtgtg tcaaagtggt gcaaagtcac gaatggctca
     2641 acatgacgtt tgtggcactc ggcaaaaaga ttatggtaga attcaacacc tttttcattt
     2701 acttcgccat agccagttgg gaaaatacga gaccaggcaa ttgaaatacg aataccatta
     2761 acgccatatt tttcagctaa ttctaaatca acaggatatt tatggtaaaa atcacttgca
     2821 ggctctgctg tgtaccaata attgtcttca agatacttat cccatgcaac tgggccttta
     2881 ccatccgtgt gagtcgcacc ttctgcttga taagcagctg tagcaccacc aaaaataaaa
     2941 tctttaggaa gtgttttagt cataactata aatcctttca aaaaaagcat ttgagacgat
     3001 attctgagtt ccggaaacca aggaaatgaa atgcaatgat atggttaaca atattataag
     3061 gaattgaaag aataatctcg tctcaaataa cttttacaaa tttattaatc aaactgttgt
     3121 tgaacaaatg caagagcacc ttgtccatca cgtgtcaatt tgatatattg agctccttca
     3181 gtttttgcaa gtttaatgcc caatttatcc gtttcagcct tcatatcatc gaagtttgaa
     3241 gcgacctgtg gagccaagat aaccaagtca aactctggca acatttcacg gtgagcaccg
     3301 taactacccg ctgccgcttt gactggaaca ttgtattctg ctgctgcttt gttcaaggca
     3361 ttggcaagca agccacttgt acctccacct gcacaaagaa caaggacatt agtttctttt
     3421 gtgatattat tgtttgctgc tacatcttct ttagcaacgc ctgcttttcc aagaacggca
     3481 tctgctttag cagtattgaa gtttgcagct actttttctt taagagcatc gtttgtttta
     3541 ccagaacgtt cttcttcaag aatttgttca tcgtatacct taacaaatgg gtaataaatg
     3601 atagtatcaa caactaccaa gagtccggct aagataaatg atagaacttg gaagttagta
     3661 ccgagtacaa ttcccaacgg accaggagta acccatggaa ggttggcaga gaatgagttc
     3721 atgttcaatg tgtcaacaaa gaatttaaag atccaaacgt tgacaatcgg agcgaaaatg
     3781 aacggtacaa agaaaatcgg attcaatacg attggtgcac caaataagat tggctcatta
     3841 accccaaaga atgttggaac aactgaggca cgtccgatgg cacggttacg ttctgattta
     3901 caaatccaca tgaacaagaa tggaacaatc aatgtagctc ctgttccacc catggtaaca
     3961 ataaacattt gagtaccaga agtgataact ttgtctgcat gttgtccagc ttggataaga
     4021 tgcaagttgg catcgatatt ggcataagtg atagcagcga ttgctggttc gacaattgaa
     4081 ggaccgtgga taccaacaaa ccagaagaag gcataagcac caaagataag tgtaattcca
     4141 aggtaaccat ctgcagctga gaagagagga gcaagaaggg taccgattga ttctgcaacg
     4201 gttacaccaa gagttccctt aacaatgagt tcaaatccat aaaggagaac aaccgctaca
     4261 gtgaatggaa tcaagtcttt aaatacttga gagatatttg gtggaacctc ttcaggcata
     4321 cgaatagtaa cattattttt cacacagacc ttgtaaacat taactgtcac aaaggctgca
     4381 ataaatgctg tcaaaagacc ttttgttccc gtgaaggctg tcaagaagcc tccttctttt
     4441 gcaggctcag ctgccatcaa aaggaaccct accatagacg ctagcatcgt agataagaag
     4501 ttaatctgat tggtcgcagg taggtcacgg ttcattgagt ccgtcaaagc tttagcggta
     4561 gtaccaccaa caaagaatgc taggataccc atcgaatagc tataaggagt catcaagaag
     4621 gtctcaatgt cttttgacca gtggaatccc caagcatttg ggacataggc aataaggata
     4681 aagatagatg agaaaaggat gactggcata ccagcgataa atccatcacg aatagcacga
     4741 agatagatgt ttcgagagat tttctcaaag aacggtttcc ctttctcaat aagttcaatg
     4801 agtttatgca ttattttgct cctcttttat aaagttcgat gaggtgatga atcacatctt
     4861 ttaataagac agtagtcatt aagtggtcct gaccatgcat catggtaacg ctgtatggaa
     4921 gttcctcccc agaagcttct cgagccaaca tagctgtctg agaattgtga gcctctgcaa
     4981 tacagcttcc tgcttctact acaagactat ctgccttagc gaaatcacca ttttcagccg
     5041 ctttaagcgc ttctaaaagt ttagagcgag catctccagc ataagcaaca atttcaaacc
     5101 ctaagagagt catctcttct ctgttcataa caatatcctc cgttttatgt ttttactata
     5161 ctttatcagt ccatggacta gctgttttaa caagaacttt gttaagttca tcaatatttt
     5221 cgaatccagt agtacgcaac cattcacgtg ctgctttttc accttctttg atgtaaggtt
     5281 ctacagatcc tgcccaagta gcacgtccac aaagcacacc attaaacttg gcacctgagt
     5341 cgtgagcaaa ttgcaatgtt tcttggaaca atttagcaga tacacctgca cttaggtaga
     5401 tgtatggaag gttagtcgct tcttcttgtg ctttaaagaa gtcagcagct tcagctttac
     5461 tgtaaactac ttcaccatca gcaaaacctt cgacatattt aacgttaact ggtacttcta
     5521 ctttaagaac atcaatgtta aagcgtggat cagagaaaac tttcatcgct tcaatgactt
     5581 tgcgaggttt cacttttgca tattcaacag aacctgcatc tgagatttct tcatcataag
     5641 caaggatttc caagaagaaa ggaatatctt cagcgacaca ttcagaacca acacgttcaa
     5701 tataagcttg tttttgttgg ttaagttcat ctgagctgtc tacatcatag taaagcaaga
     5761 acttaacggc atcagcgcct tgctctttga tacgttttgc agaccaaaca tccaagcagt
     5821 caggcaaacg ttttgtactt gatgtatcat aacctgtttt ttcataggca agaagaagac
     5881 ctgcttcttt atccaatgct tttgttgctg gaagtccata ttcagggtca agcaacattg
     5941 aagaagcgta ttttgtaagt tcatcagcaa ccaaaacctt tagttcttcc atttgagcta
     6001 cagttggttc tgtatcttga tactgtgcca tcaaacgttt caaagcacca cgttggtcaa
     6061 atgccaaagc tgagataaca ccgtttttat ctgaaatttt ttccaaagat ttgcgtttct
     6121 gttctgtaag taccattttt atacctcttt tactccaatt tgtgagttta gtgtttcgta
     6181 attagtcata ttcacatgcc ctgtcattgt ttcttgagca tttaacatac ccaatgtcat
     6241 cgcatgtttt aataagtcag cgtcactttt tttactattt aaagctgatg caataccagc
     6301 taccgttgag tcccctgatc cgactggatt aacaactggg atatcaggaa tatctacctt
     6361 atagaaaaca tcaccatgtt ttgcaaaggc accgtttcga cctaatgaga ccactatcca
     6421 ttcgatacca ctaaaaagtg actctttaag aacgtctttg agttcttcaa tatcttttgt
     6481 tacttctttt ccaagcaact gtgaaagttc ctcattatta ggtttgatgg cagtaggttt
     6541 agccgacgat tttagaactg tttcaagagg tgcacctgaa caatccaaaa cgactgctac
     6601 tccttcatcc gaagcaagtt ggataagttt ttcataataa tcgttgggaa gtcctgaagg
     6661 taaacttcct gaaattgtca caacttctga ttgtttaata agattgctat agtggtcaag
     6721 aaagccttct gcttcttcat gagaaattgt tggtccttgt tccaaaattt ctgtttggtt
     6781 accctcgtgt aagatagcaa tacagtttct agtgtttcct gagattttat aaaaagctgg
//
LOCUS       DAPCMU010000132         6807 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPCMU010000132 DAPCMU010000000
VERSION     DAPCMU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.136"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            15..548
                     /locus_tag="RD744_002291"
     CDS             15..548
                     /locus_tag="RD744_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558272.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(674..1351)
                     /gene="nth"
                     /locus_tag="RD744_002292"
     CDS             complement(674..1351)
                     /gene="nth"
                     /locus_tag="RD744_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDU0558273.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1358..2056)
                     /locus_tag="RD744_002293"
     CDS             complement(1358..2056)
                     /locus_tag="RD744_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDU0558274.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2324..3508
                     /locus_tag="RD744_002294"
     CDS             2324..3508
                     /locus_tag="RD744_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDU0558275.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3514..3957)
                     /locus_tag="RD744_002295"
     CDS             complement(3514..3957)
                     /locus_tag="RD744_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0558276.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4183..4470)
                     /locus_tag="RD744_002296"
     CDS             complement(4183..4470)
                     /locus_tag="RD744_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558277.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4641..5993)
                     /locus_tag="RD744_002297"
     CDS             complement(4641..5993)
                     /locus_tag="RD744_002297"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDU0558278.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6266..6712)
                     /locus_tag="RD744_002298"
     CDS             complement(6266..6712)
                     /locus_tag="RD744_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDU0558279.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1857 a         1331 c         1108 g         2511 t
ORIGIN      
        1 aaaaaataca atatatggtt ttgaataatt ttttgaaaga aggcttcatt ttgagttatc
       61 tgatagtatg cttaattatc ataatttgtc tgcaccatct ttttttatcg aaaacaatac
      121 ctcgttattc caagaataaa aaagctgaaa aattttgttt attattaaat aaatttactt
      181 taattgctcc tatccttgca tttattattt tttctgtact tttaagcact actcttaaag
      241 gaaaatttat ggaaagaagc tctcatgcaa tgattctcac ttttttatgg ttgttgttca
      301 ccagaatcta tatttttctg atgagcctga aaccgcccaa atccattagt ttatgtttag
      361 ttataaatgg tattttttta ttatcactta tcatttttat tacaccttta gatagatacg
      421 tcacttattt atataatcct ttggaatact ggacttattt tataggtata ttggaaggca
      481 ttattttcta catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata
      541 aattgtaata agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa
      601 agataaacga aacagctttt ctccataata acaagataat tcatacattt atttgcaatt
      661 aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc
      721 gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg
      781 ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt
      841 gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga
      901 tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga
      961 atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt
     1021 cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt
     1081 tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat
     1141 cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg
     1201 ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa
     1261 atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta
     1321 gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca
     1381 gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct
     1441 gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt
     1501 cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt
     1561 cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga
     1621 tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca
     1681 ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat
     1741 actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga
     1801 caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa
     1861 tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc
     1921 cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga
     1981 tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat
     2041 tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg
     2101 gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt
     2161 ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa
     2221 attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta
     2281 tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa
     2341 taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat
     2401 cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc
     2461 tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat
     2521 tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg
     2581 atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg
     2641 tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga
     2701 catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat
     2761 aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc
     2821 tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct
     2881 agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt
     2941 tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt
     3001 tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc
     3061 ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac
     3121 tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac
     3181 aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct
     3241 tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt
     3301 tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt
     3361 aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg
     3421 acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt
     3481 tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct
     3541 gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac
     3601 agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg
     3661 tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt
     3721 tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta
     3781 actagcattc gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac
     3841 ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg
     3901 aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc
     3961 tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa
     4021 tattttgtag gaaaacccaa tctgaaacca agaaaaaaag gggttgacaa agcttgtcat
     4081 aacccctttt tctgattaaa agatagtaaa gagacagtca tcggtaattg tgatgatttt
     4141 ttgtcatcac ttaaggatga gcggcctttt caaaaactgt ctttaaagat catcatcagt
     4201 ttctagcaca tatggctgac cttcatatgt gtactctgta ttcaatgtgc cgtcattttc
     4261 atctgagtag atgcgtctac tgaaaggaaa acgatgagaa acattatctt tggcgtcatt
     4321 tgctggcatc gtgtaatatt cctcttgcgt ttcattcaac tgagagagta cttgatctgc
     4381 caactcatca atattgatcg ttaatttctt cgaagcactt ttataatctt ctaaaaatgc
     4441 atcttgttgg acaaaatatt ctttattcat cttctttctc acctttcttt tcaaaatcaa
     4501 ataaagctgc ttgttctttt atgtaacagc tttatctttg ctgcttattg tgacaaaaaa
     4561 ttcgaagaaa gacaactaat tgtacttttc acaaaaaaag acagagggtc gtgacaaaat
     4621 tgtgtcacaa ccctttttct ttattgtgca gtttcgattt cgcctagcgc tgcttgtgca
     4681 agtaggttca agtaattcca tgggcggtca aaatgtggtt ggaagaagaa atctgaaagt
     4741 gcgagatctt ctacagtcat tttattttga acagctaaag acatggtatt tgctgattgt
     4801 gtaatgtcgt atttggacat gaactgagca cctacgatac gattcgttcc tttttcatag
     4861 actagctcca tcattacttt ttcagtagtt ggcatgaact ctggacgata attatcttca
     4921 aatacagtcg cttggacttc tagatcgttc aaagttgcgc tttctttggt cacacctgtc
     4981 gaaccgatcg tccagccaaa taggtaaagt cctgaagttc cttgagtacc acgatacgcc
     5041 attttatgat ctgtcaagtt atttcccaca agcaaacctt gacgaacggc atttgttgcc
     5101 aaaggaatat agttttgcgt gccacttgga ttgtagttga ctactgcaga gtctccagct
     5161 gcaaaaatgt ctggattact tgtctgcatg tattcattca ctttgattgc cccgtttggc
     5221 agtgtatcta cttttccttc gatcagtttt gtatttggac ggaagcccac acataaaatg
     5281 accatatctg tatcaaatgt atctgttggt gtagctactt gtttcacttt tccttcttca
     5341 tctgtgataa attcagtaac attttcgcct agcgcaagag taacaccgcg gtctgtcaat
     5401 tctttttcta agatatctgt gaatggttta tctagatatt tattcaagat gcgatctaat
     5461 ccatcaatca atgtcacttg ttttccagat tcgacaaaag cttctactaa ttcaataccg
     5521 atatatccac caccaacaat tactacacgt tttgcttctt tcgcttgtgc aataatttca
     5581 tttgcttgat tgtagttttt gcataggagt acatttttcg catcgattcc actaatcggt
     5641 gggataatag gccatgaacc agttgtcatc actaatttat cataagaaac cgtttctgtt
     5701 tctcctgttt tcagatcttt tgccgttact gttttatttt ctgtatcgac gtctgttaca
     5761 tcgtgttcca ttttaactgt tgctcctaat gaagataatt cttcaggatt tgagtagaac
     5821 aagcctgctg ggtcctttac tactccgccg acataaagtg cgataccgca agataagaaa
     5881 gaaatattgt catttctttc atatactgtc acttctgctt ctggatgatt ttttaaaata
     5941 ctttttacag ctgcagtacc tgcatgagta cagccaatta ctactacttt catttatggt
     6001 ccctccgatt aacattttgt ccttcgacaa aactactata acagaaataa aacggataca
     6061 acagtttaat gcttgtgaat tcacaaaggt ttgtttgtag tttcattcct tttcaagcat
     6121 aagaacccaa tatctacaag attcttattt taataatatt cacttttata tctaagattg
     6181 tgaataaaag acaaaaaaat aaaaaagcta gcgttttttt tacacagcta gcttttttta
     6241 ggtataacac ttgtagaaac tgtttttaca tttgcgcctt ttttgctgac tctttacaat
     6301 ccggacagag tccatacaat tccaaccgat gttcattaat ctcaaaatca gtcagtctgc
     6361 ttgcagccat ttctatatct tcaagccctg ggtaatgaaa atcaacaatc ttaccgcagt
     6421 tttcacaaat tacatgataa tgacgctttg aactgaaatc aaatcggcta gaagcatcgc
     6481 cgtacttcat ttcttgaaca aatccaatcg aagtaaataa gcgaaggtta ttgtaaacag
     6541 tcgcaacact catatttgga aaccgatgtt caagtgcttt ataaatctca tcagccgttg
     6601 gatgggaatg atgttcaatc aaatattcta aaaccgcgta ccgttgaggt gtgattcgaa
     6661 tgttctcttt ttttaacttt tcgataacct cttcgactaa cttattttcc atgtaaacac
     6721 cccacgttgt tattctcgtc ttatcatact atttttacat tttaaaatcc acttaacatg
     6781 aaaagaaaat tcaattttat tgtattt
//
LOCUS       DAPCMU010000133         6706 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPCMU010000133 DAPCMU010000000
VERSION     DAPCMU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.137"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(7..1164)
                     /locus_tag="RD744_002299"
     CDS             complement(7..1164)
                     /locus_tag="RD744_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558280.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="RD744_002300"
     CDS             complement(1167..1523)
                     /locus_tag="RD744_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558281.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="RD744_002301"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="RD744_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDU0558282.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="RD744_002302"
     CDS             complement(2991..3830)
                     /locus_tag="RD744_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0558283.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="RD744_002303"
     CDS             4015..4902
                     /locus_tag="RD744_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDU0558284.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="RD744_002304"
     CDS             complement(5486..6181)
                     /locus_tag="RD744_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDU0558285.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="RD744_002305"
     CDS             complement(6165..6563)
                     /locus_tag="RD744_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDU0558286.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2108 a         1319 c          987 g         2292 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DAPCMU010000134         6609 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPCMU010000134 DAPCMU010000000
VERSION     DAPCMU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.138"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..2614
                     /locus_tag="RD744_002306"
     CDS             <1..2614
                     /locus_tag="RD744_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDU0558287.1"
                     /translation="YVNLRTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNIT
                     YNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERY
                     LKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDT
                     SVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMM
                     STSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHT
                     NNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYF
                     DQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEG
                     GCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQ
                     LQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGY
                     LNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASN
                     NQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEE
                     RLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQG
                     ITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTV
                     LINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNG
                     DIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTY
                     EILLPSSLKSYFRL"
     gene            2796..3035
                     /locus_tag="RD744_002307"
     CDS             2796..3035
                     /locus_tag="RD744_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558288.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3051..4424
                     /locus_tag="RD744_002308"
     CDS             3051..4424
                     /locus_tag="RD744_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558289.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4428..6062
                     /locus_tag="RD744_002309"
     CDS             4428..6062
                     /locus_tag="RD744_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558290.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6225..6431
                     /locus_tag="RD744_002310"
     CDS             6225..6431
                     /locus_tag="RD744_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558291.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
     gene            6500..>6609
                     /locus_tag="RD744_002311"
     CDS             6500..>6609
                     /locus_tag="RD744_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558292.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         2325 a         1079 c         1327 g         1878 t
ORIGIN      
        1 ttatgttaat ttgagaactt tttctacatt aaacaagaaa accgtttctc atattttttc
       61 tactgcagat tctcaagcaa ttattaacta tttagaaaaa cagaaattgt ctgaaggtac
      121 agtaaacata acttataatc ctattaaaca agaggtgatt acacaaaaaa agcattctgt
      181 gagagacaat gatattgaaa aaattcggtt tgtatttatt gatattgacc ctaaaaggaa
      241 agagggaagt gcaacagata gcgaaaagaa aaaggcagaa aatgttatgg aacaagtaga
      301 acgttatttg aaagaaaaga gaatagaatc atttgtaaaa gttgatagtg ggaatggcta
      361 ccatattttt cttcccataa atgaacaacc aaataatcac gagacgattc ttactattca
      421 gaattttctt caattacttc accgaagatt tggagtagaa gatgaagttg atattgatac
      481 ttctgtttat aatccttcaa gattatgcaa attagttggt acaccagcgg tcaaaggaaa
      541 gtccacaaaa gagcgtccgc atcgtttatc tagtttcctt tctattcccg ataacataga
      601 tagaagtaat tgttttaaaa ttcttgaaca gatagttgaa gaaaattcca tgatgtcaac
      661 ctctagagta aaaagccatc catcttttag agaagtgtta ccttttattc aagcagacta
      721 taaacaatgg ttagctcact atcctgaatt aacttatacc ataaaagagg gcgatcataa
      781 aggagttacc ttacttatat ttgacgaatg tccattaaga aaacacacga ataattctaa
      841 tggttcttgt ttgtctgtta cggctgataa taagattcga tttcattgtc tgcatgcttc
      901 ggataaaaat aaagatattc aagactttgt aaaaaaatat ccgttgccaa aacctgtaaa
      961 tccttctgta gaatcattag aatccaatca agaatattat tttgaccagt ttaaactgaa
     1021 tcaagaagga gtatttttgg tgaaagaagg tcaagaagaa aaagttgctt gtccgatgtt
     1081 tattaagcaa atacgcaaaa atatcgaaca aaaagtcatt acacttgttc ttgcctatta
     1141 tgtcgatcat tgttgggatg aaatagaaat agaaggtgga tgtttacaaa ctaataattt
     1201 taaacaatta gcaaaacatg gcttaatcat taaagctaga agagaagctg aagtcgttga
     1261 ctttttacag attcaacggg agaatgctcc aatctttttt gaacatgaat ctcttggctg
     1321 gaaaaaagaa aatgaagcaa tgatttttca gttacaagat tcttttggac gtgaaattcc
     1381 ttctgtatta actaaaaact catgctacca attaacaact aatggtacag aagcggaatt
     1441 tgacgcattg gttcataagt atgttttagg aacgaactta gaacttgctt ggtcgattgg
     1501 tattgcttct attgtgttag gctatttaaa cgttacgaag gctaatactt ttttgagttt
     1561 actaattaac ctacagggca agagcacaac gggtaaaacc actacgttac actttattgc
     1621 ctctttattt ggaaatccaa ctacggttct tagaaatatg aatgctacca ataaagccat
     1681 tataaagcta gcatcaaaca atcagggagg tgttccacta atactagatg agttaggagc
     1741 agcaactcaa aataacttta gctcgttgat ctatcaatta gcttcaggag aagaaaggct
     1801 aagattagat tctaattgtc agcttatgcc acaagagact ttttctgttt tgacccttac
     1861 ctcttctgaa gagcgtttgg aaaattattt caatgagaaa ttaactggtt tgaaagttcg
     1921 atatatcgaa ttttctgata tcaagtttac aaaggatgcg aatcacgcag agaaagttaa
     1981 aagtctttct tctaatactt ttggttgggg tataaagtta tttcttttga aactctttga
     2041 acaagggatt acaaaaattg aagcaacttt tgtcgaagct caaaaaaaac tatgggctgt
     2101 tttacctgaa cattctttga agcaacgcat tggtaataat ctagctttga tttatagtgg
     2161 agccctccta attcaagata tactgggcta tcctttacga ttagagcata tacagacggt
     2221 gcttattaat tcgtacgaac agttaattgt agatactagt ttaggaagtg agcaaacggt
     2281 atttgaccga gtgaaaacat tgttggtgat gaatagccat aaatttttaa caaacagtga
     2341 caacaaacat acgcccagta cgatttgggg aaaagtatca attcaaaaaa atggggatat
     2401 taaggtaaat atttttccta tcccgtttga ggacttgtta aggcgagaat ttcaagtaaa
     2461 ggatctaacc tatcttttta ggcaattgct tgattcgggt aatatgtgtg cagaaaaagg
     2521 gagaagaacg aaacgtattc acatgaatcg tcaagttctt ccaacatacg agattctatt
     2581 accttcttca ctcaaatcgt attttcgatt gtaatttaga ataaaccaaa gtatgaaagt
     2641 gaggcaccat gttatggaaa tatttcttat tgatttagca aaacaactga ttacttatct
     2701 gtatgatagc gagcaaaccc aaacggaagt aaatgaagct tgatctatgc ttcatttact
     2761 tcattcaatc aaacatgaaa gaaaggaaaa ttctaatgac taaaaataaa atgacactaa
     2821 aagcagaggt tcttctgtat attcaagaac atttttcaaa tcaagccttt tttacaaaac
     2881 caatttactt ggatttcgaa ataagagggg tatctgctgg atctatcggg ggtactcttc
     2941 aagcgttgaa gaacgaagga tatttagaaa atcactttgt gcaacgttcg ttcaatagaa
     3001 gagtcgtgaa aaaatggtat ttagttcatt catagagagg ggaatcaagc atgcgtgtgg
     3061 cttgttatga tcgaacgtct gtttatgaag aagcgaaaat tagtaatgac gctcaatatg
     3121 atgcagtgaa agagtatgtt ttagagaaat atgaacaaat tccccaacga ttttcagaca
     3181 aaggagaatc aggggaaaaa gcggagcgtc cagcttttcg acagcttaga gcttgggttt
     3241 gtgcgtcaga agaagagggg gtgatctatg tttggcgtta tgatcgattg gcacgtcaag
     3301 ccaaaatagc cttagaattt attgagttgt gccaacaaca tcaggttcaa gtgtattcga
     3361 tttctgaacc tcttcctgct tctttctcac acgcgtcaac gaataaacta tttgtgcaat
     3421 tattgttcac ttttgcagaa caacaacgaa acgtcattat tgagaatgtt cgtgccggac
     3481 tttcctataa aaagcaacaa cggcaatatt tgtcttctgc ggtaccatat gggtatcgtt
     3541 tagtgaatgg gaaaatccaa caagaagtac acgaagcaag gattgttcgc tatatttttc
     3601 aactttattt gaccaaaaaa tatggctaca aaaagctttg tcaacgattg acgcaacaaa
     3661 agcttttctt ccgcgaacgt ccttttcagc cctatcatat ttatagtatc ctaaaaaatc
     3721 cattgtacta tggcgaaatc aaaggaggat ctttggggaa gtatctagga acctttgaac
     3781 cgattctctc gaaagcaacc tttttacaag cccaagagat tcgccaatca aggtgtacag
     3841 ctaaaaaaga tacgtatctg tacttattac gtcaaaaaat tcggtgtccc ttctgtggtc
     3901 gtcatctgag ttcgaagtat caatggaata caaagaagac gaagacgctt cattattatc
     3961 attgtaccga ccgaagctgc caaggaattt ttcttcgggc agaaagggtt gagtcagaag
     4021 tattggaaaa gatacaggct ttttttcaat acgaaggcgt ctatcaagcc ttgattgccc
     4081 aattaacgca acaattagcc gaaaagcgaa aagatgaaaa acgcaaagta atccaacaaa
     4141 agaaacaaga agcacaatgg attaaggcat ttgaagaggg caattgttca ctggaagaac
     4201 tcaagcaaca actgcaagct cttcaaaagc cgatgtctac ttcttcgttt ctttcactcc
     4261 aacagtgtga agcaaaagtg aaagaatggc ttcaactaaa agacctctct agtcgacaat
     4321 tattatgtga ctatgtttgt cggattgagg taacaaagga taaaacagtt tgtgggattt
     4381 acttccgaca actgacacaa aatattttag aaaggaacga atgatctatg gaaatgatac
     4441 aaaccaaacg agtcgcattg tatattcgcg tgagtacaca agaacaagct ttagaaggac
     4501 atagtattca agcacaagaa caaaatggac gacagtttgc cgaacggatg ggctatgaag
     4561 tggtagaagt ttatgccgat gaaggaatca gtgggaaatc cacaaaaaat cggttcgcct
     4621 atcaacgaat gatgaacgat gctcggaacc atcgttttga tctactagtg atttggaaat
     4681 tgacccgtct cggacgcaat atgttagatg tcttacaaac agtcgaggaa ttattgcgat
     4741 atgacgtagg attacattcg atctcggaac aatttgacgt gaccacttcg acaggtaaat
     4801 tgatgttaca actccttggg agtttcggtg aatttgaacg caaccaaatt gcggagaatg
     4861 ttcaaatgtc aatgcaaagc ttagtgaaag ataaaaagcg ttatgcagga ggccgacgtt
     4921 taggctatgt cagtggtatt gacacgaatg gtcaaaaaca attggtcatt gaaccagaag
     4981 aggcgaaaat tgttcgtctg atctatgcaa aatatttagg cggagcaggg tatcgagcga
     5041 ttgccaattt tttgaatcgt caaggatatc aaacagtcaa aaagaatgct ttttctacca
     5101 cggcagtcaa agatatctta cgcaataaaa tttatgcagg gtatttagaa tatgcgcgct
     5161 acatcaattg ggaaaccaaa cgacggaagg ggaagaatcc gagaccgatt ttagtgaaag
     5221 gaacccatga accgattatt tcggaagaag attatcaaaa agtacaagaa cgccttgctt
     5281 tagaaggaaa acaacccaaa tggaaccata caggtgaaaa tgtattgaca ggcttgttac
     5341 gttgtccaga atgtggcgca ccgatggcag cgagtaatgt cacaaatacc ctaaaggatg
     5401 gcacaaagaa acggattcga tattattctt gttcagtctt tcggaataaa ggagcaagtg
     5461 tgtgtcatgc caattcgatt cgtgccgaac aagcggaaac atttgtcgcc gaccgattaa
     5521 aggagatcgt tcagctacca gaagttcttc cccgattggt ggcagcgtta aatgaagaaa
     5581 tagtgcgaca aagccaacca ttggaacaag aactcgtggt gttactggaa cgaaaggaag
     5641 aattgaagac taaaattgaa aaatgggaag ctgctttgga agatagtcct gaattgtttc
     5701 cgatgttaaa agaccgctta gacgaattga ctgaaaaacg acgacagttg cacattcgag
     5761 aaaatgaaat cttagggatc tttcaacaag gagagccgat tcaagtcaaa gatgtccaac
     5821 ggatattgac taatttggat cgttttcttg cccagagtga gaagaagcaa atcaaagcct
     5881 tgtatcgtac ctttatagag aaaataacct ttgatcctaa ccataaggag atgttggaaa
     5941 ttacgatgaa attcacgcat gccgtggtac aacaattgaa tgagcaatat caaatagccg
     6001 tatccaaaac aaaggatacg gctatttttg tgttaaaaac accctttacg ttaacgatct
     6061 agtctaggtt atatcaaaca acgttagagg ggcgctaacg cttctctagc gatttgaaat
     6121 cgttagagaa gtagttaggg aggtccttat cataaatagg gatatttatt aatgaatatt
     6181 tgaaatataa ttgtatataa gaattaaaaa gagtggggat agatatgaaa aagatgggta
     6241 agtgtatttt atgtgaaaaa tacactgaac ttactaaaga acatgtacca cctaaaaaat
     6301 cagggaatac tggaggtaaa aagaccagaa ctggtaattt agatgatttc cttaagagcg
     6361 attttacaaa aggtgatttt ccgaagggga ttaaaaggaa acctcaagga aatgtttatt
     6421 atactggata ggctacaatt aactagacag aaaaattaag gtgtgtagac tagaagaaaa
     6481 cataccagga ggaattttta tgtctaagag aacacgaaga actttttcac aagaattcaa
     6541 gcaacaaatc gtcaatcttt acttagctgg aaagccacgt gtagaaatca ttcgagaata
     6601 tgaactaac
//
LOCUS       DAPCMU010000135         6524 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPCMU010000135 DAPCMU010000000
VERSION     DAPCMU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.139"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1429
                     /locus_tag="RD744_002312"
     CDS             <1..1429
                     /locus_tag="RD744_002312"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDU0558293.1"
                     /translation="YDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNV
                     LADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVI
                     KKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKE
                     RRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEE
                     MNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKA
                     DFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFF
                     GTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEK
                     YAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEA
                     ISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1419..2636
                     /locus_tag="RD744_002313"
     CDS             1419..2636
                     /locus_tag="RD744_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDU0558294.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2649..5798
                     /locus_tag="RD744_002314"
     CDS             2649..5798
                     /locus_tag="RD744_002314"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HDU0558295.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2284 a         1082 c         1333 g         1825 t
ORIGIN      
        1 atatgacaca gtaagcaaac aaacaatgtt gtatcgtgaa ttactttcag atgaagaatc
       61 aaaagaagat ctgattgcca caatcgttga tattttggga tatgcaattg caccagagta
      121 tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg ttccaattga acgatctaaa
      181 caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat ttgatgatgt
      241 ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga cgattaccga
      301 agtcatcaag aaattgaatg atgtagatgt tttagaacac gatggggacg tgatcgggga
      361 tgcgtatgag tttctgatca gtcaatttgc ttcagaagca gggaagaaag ccggagaatt
      421 ttacacacct catatggttt ctgatatgat ggcgcaaatc gtaacgctcg atcaaaagga
      481 acgacgattc tttagtgttt ttgatccaac gatgggttca ggttcattga tgttaaacgt
      541 acgtaactac ttaacacacc cagataacgt taaatatcat gggcaagaac taaatacaac
      601 gacatacaat ctagcaaaaa tgaacttgat tttacatggt gtcgacgccg aagaaatgaa
      661 tctccgcaat ggggatacgt tgaataagga ctggccaaca gatgaacctt acacatttga
      721 tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga ctttcttaga
      781 tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa tccaaagcag attttgcgtt
      841 tcttcttcat gggttctatc atttgaagga aacaggtacc atggccatcg tcttaccaca
      901 cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt caaaaattac tagaagatgg
      961 cagtatttat gctgtgatcg gtatgcctgc gaacttgttc tttgggacat cgattccaac
     1021 gacggtgatt gtgttgaaga agaatcgtca aactcgagat gtattattca ttgatgctag
     1081 tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg gaagaaaaca ttcaaaaaat
     1141 cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac gctcatttgg caacctttga
     1201 tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc tatgtagata ctttcgaaga
     1261 agaagaacca attgatatgg ttcatgtcgg aaatgacatc aagaagatac gacaagaaca
     1321 gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc ctgcaaacaa cacctgaaaa
     1381 tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat gagcaatgat acacaaccag
     1441 aaatccgttt cccaggattt acagatgatt gggaacagag gaagttgggg gacttcgttg
     1501 tagattatgt tgaaaagaca agtgtacaaa atcaatttcc tatgctaact tcatctcaac
     1561 aaaaagggat agttttgcaa gaagactatt ttgcaaatcg gcaagtaact acagaaaata
     1621 atattggtta ctttgtacta cctagaggat atttcacgtt ccgtagtcga agtgataatg
     1681 atgtgtttgt gtttaaccgt aatgacatta ttgatagagg gattataagc tatttttatc
     1741 ctgtatttac tttaaaatct gctgattccg acttcttctt aagacgtatt aacaatggaa
     1801 tccaacgaca attatctatt caagcagagg gaacaggtca acatgttctg tctctaaaaa
     1861 agtttaaaaa tatagtagca atgtttccta gtgaggaaga acaacaaaaa atcggcacct
     1921 tcttcaaaca acttgacgac acgatcgctc ttcatcagcg taagttagat ttgctgaagg
     1981 aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa cggagcaaaa gtgccagaaa
     2041 tccggtttcc tggttttacg gaagattggg aagagcgtaa gttgggagaa ttaggtaaaa
     2101 ctcaatcggg aattggattt cctgatgccg agcaaggtgg ttctgaaggt actccatttt
     2161 ttaaagtttc cgacatgaat aacattggta atgagtatga aatgagaaat gcaaatcatt
     2221 atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc aataaaagat gtgccagcga
     2281 ttatatttgc taaagttggc gcagcaataa tgcttaatag aaaacgtttg gtcacaagcc
     2341 ctttcttaat agataacaat accatggctt atttatttaa taatacttgg gatatctatt
     2401 ttggtaaaat attgtttgaa actataaatt tgccaagata ctcccaagta ggggcattgc
     2461 cttcttataa cagttctgat attgagaata tcagtgttaa ggttcctgta aaggatgaac
     2521 aacaaaaaat cggtactttc ttcaaacagc tagatgatac gatcgctctt catcaacgta
     2581 agttagattt actaaaagaa acgaaaaaaa gcttcttaca aaagatgttt gtctaggagg
     2641 gaagaagaat gaaaattagt caccgtgagg aagcagaagt cgaagaacag ttgattcgtg
     2701 tacttggaga agggcataat cagtggacgt atcgtccgga tctaaaatca gaagaagacc
     2761 tttgggtcaa tttacgccaa aaaattatct caaataatca agcagaactg aatgattttc
     2821 ctttgaccga taaagaattt gaaacgatca aaacggaatt actgctgcga acgaaaaccc
     2881 catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat ggctcggatc acgattgaac
     2941 gggaagattc gcaattgggt tctgtctcac ttatccttta ttccaatcaa gacattggtg
     3001 gaggaatctc tacttatgaa gtggtccatc aaattgcaaa acgtggaagt aacatagaag
     3061 cacgggatcg acgttttgac gtcaccttat taatcaatgg attaccgatt gtccaaatcg
     3121 agctgaaaca agtaactgca aaagatggtg tttatcaggc atttaaccaa atcaaaaaat
     3181 atgcagaaga aggtatgttt cggaacaata tcttttcgac cttacagttg tttgtggttt
     3241 ccaatgaaca aaccactcgg tattttgcaa atgccttacc gaaagacttg caccctaagt
     3301 ttttatttag ttggcgaacg aaagacaatg aaaaagtaga aaacttatat gagttttgta
     3361 aacaagtgtt aaatatcccg gatgctcatc gtttgattgc ggattataca atcgtcagtg
     3421 aagatcaaga caataaaacc ctcatggtgt tacatcctta tcaagtacat gcgattcaag
     3481 ccttgtttat tgccgcaaat aaacatcaat cagggtatgt gtggcatgcg actggttcag
     3541 gaaagacgtt gactagtttt gtatctacta agcttttagc acgaaaatcg gggatcgatc
     3601 gaacgatcat gttagtggac cgcaaagatt tagataatca aaccaccaca gagtttacaa
     3661 aatttgcttc ggaatttaat actgggattt cttccgggaa cgccaaagcg aatagcttga
     3721 tcgttggaac gggaagtgcc aaagaattaa gtgaaaccct gttggcagat gccaatgcaa
     3781 atgttgtaat cattacaacc cgtcaaaaac tagatgcggc gttgaaatat gcaaagaaac
     3841 aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat gggccaacat atcgtgtttg
     3901 tagtagatga gtgtcatcga gcgttaagtg ctgaaaatat ggaagaaatc aaaaaaatgt
     3961 ttccgaaatc cacttggttt ggctttacag gtacaccgat ttttcctgag aatcggaaac
     4021 aagccaaagg gcaattggca cgtacaaccc atgatcagta tggagaggtc ttgcatacct
     4081 ataccattaa gaatgcgcta gaagatggct ctgtcttagg atttcaagta gagcatgaaa
     4141 acaccgtgga gccgacttca ctagagaata aaatctatcg gaaattaaaa gaagtagaaa
     4201 catatgcaga gtattcatca gaacagatca atcgtatgat tgatcaaatg gaacctgtca
     4261 aaaaagaaag ttatttggac ccagctgttt ttgaagcaga tgaacatatc caaaaagtca
     4321 ttcataaaat gttccgtcca gataatgcct atacaaaatt tgattttcga aatggcaggc
     4381 caacgaaatc tgcaatctta accacgagtt ccattgacat ggccaaaaag tattatcggg
     4441 ccatcaaaga gatgacgaaa gagcctgatt ggttaacaaa agagtttccc aatcagctca
     4501 ttcgtgaagg acgaacgatg gaggatcctg attttccacg aatcgctatt acctattcat
     4561 tagaagaaaa tagccgagat tcaagtgccc aacaagaaga aatgcaaaaa attattgagg
     4621 aatataatca gtattatgat acttcctggt cattagcaga cattgagcgt tataacggag
     4681 acatcaataa tcgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg
     4741 atttagcgat tgtagtggat cgtctattaa caggttttga cgcccctacg attcaaaccc
     4801 tatttgtgga tcgtaatcta gagtatgcag gattgatcca agctttttca cgtacgaatc
     4861 gtacgtatcc tgataagaca aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg
     4921 agaagaatgt cgaggatgcg acaaaactgt attcaaaagc aaaagaagaa tcaggtttga
     4981 tttatccaag ttatcaggaa tccaaaaaaa gattcaaaca agcatatggt aagttaaaag
     5041 aggtttcatc catggaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg
     5101 tcaaagcatt tcaagaattg aatcgtgctt atgaatcgtt agtgacttat gacgattaca
     5161 atgatgacat ggtgaaatct ccaactttga acaatcaaat tcaacttttg gaagaacaaa
     5221 ttggcgtgta cgagaccgtg aagggttcat taatagagga agaaccagaa aaagaaggag
     5281 aagatttttc aacgatcgaa ttttatagtg aaaataccac taagttgtat gatattgatt
     5341 caacttatat tgatcaatta ctaggaactt atgctgcgaa tagttcagat gcacgagaag
     5401 aaattgaaaa agctttggct aaattgaata aaacagaagg agtcaaagaa gtttaccgac
     5461 aaattttaaa tgcatttgat gtggggactt tagatagtaa tgaagatatc tttgtcatta
     5521 aacgtcgcta ttttacacaa gcgagtgatg atttgattca tgtcttttcc aatgagtggt
     5581 ttgtttctga gaaagaatta catgcttcta atctgcaata tctaccaggt gaagatccaa
     5641 ttcctaatat gaaagcaatt atcaacagta aggattacga aggatataaa gccaaacatc
     5701 cagaagcgaa gccatttaaa tacccacaag aaatgaagcg agcatggcgt aaaatgttag
     5761 atgatgagtt gattccactt gagaatgaat tgaggtaaac gagaagagtt aagaaaattg
     5821 tacgattcaa agatggtagt caagtggaag tgtagaaaat agggatagat gtcttttatt
     5881 tgttcacatt ttataacgaa gtatttaaga cagctattga ataatgtttc ctccaccttt
     5941 ttcattcatt taccccaccc cgttgttata tgaccgtgtt ttgagaggaa ccaataaaaa
     6001 gcataggcta actaggggtt cctttttctt ttgttttttt ggaatccgct taagtgaaat
     6061 gtatctgaaa tgaaacggag ataaagtgat tttttgctat aatgacaagg ttttagctgt
     6121 ttcagatacc atgtaaccca aaatagttta ttgagaaaat aggagagtat ttgatactaa
     6181 ctagtaggaa aaaatgagat aaaaattaaa tggaatcatt tcaggtaatc tcaaataaaa
     6241 aaatatacag tcctgtggca ctttacctat gggactgtat attttttact ctcagcgggc
     6301 ttgttttgac tccgtgtctg actccgtcgt attgatttta aatgatattc gatgaccatt
     6361 taacaggtca tttttgaaat ttttgtctct atatgactca gtttgataat tattgataga
     6421 tcaatttggg tctatatcga acaagggaat aatccaataa aaaatgattt agaatatcaa
     6481 aataacaaaa aagaggacta cctgtattcg tcctcttttt tgac
//
LOCUS       DAPCMU010000136         6500 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPCMU010000136 DAPCMU010000000
VERSION     DAPCMU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.140"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(1146..1811)
                     /locus_tag="RD744_002315"
     CDS             complement(1146..1811)
                     /locus_tag="RD744_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558296.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1991..3973
                     /locus_tag="RD744_002316"
     CDS             1991..3973
                     /locus_tag="RD744_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDU0558297.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3977..4615
                     /locus_tag="RD744_002317"
     CDS             3977..4615
                     /locus_tag="RD744_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558298.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4643..5071
                     /locus_tag="RD744_002318"
     CDS             4643..5071
                     /locus_tag="RD744_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558299.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5528..6439
                     /locus_tag="RD744_002319"
     CDS             5528..6439
                     /locus_tag="RD744_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDU0558300.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2242 a          982 c         1203 g         2073 t
ORIGIN      
        1 ggtaagtttt tccttgtttg ttttagttta aactagtgtg taatttaaaa atctatatgg
       61 aagggtctgt ggggtaatac ttaaataaag gctaggaacc tgataacatc aaggttccta
      121 gcctttttat aataaaatga agcgaaaata atttctttta aagagccgag attatcagct
      181 atataagagt gaatttgatg caaaaaacag agccgaaaca gtcttggtgt tcgtagctct
      241 gcttatatgt ttcgaattta agacaaagag ttggcaaaag atgttgataa ggaagataag
      301 ataactgtgt tatataaact aggtaactaa ctgtaacaag ctaattttaa gagattctta
      361 ttagaagacg tttaggaata taactaagaa aatgattata caggtaattg tcatttcaat
      421 aatgttttct aattttcgcg tctttgtttt aagaaaaagg ccagaatcag ttggaaaaaa
      481 gttcgtacga tagtgaagag gggcaaaatt tcctactaat attcccacta acaagagata
      541 aaacggccag gttggtgtga ttcctctata aaaaaatgcc catgctaccg aaatcccata
      601 gattaacaac tgaacaattt ttacaatgaa aaaattgttc tgccattact caaagtctct
      661 tcctccttgt atcgtttatt tgtttcattt tatcattatg ataggcgctt gaacaatcca
      721 ttttgtattt tattgagtac tcttggataa actaccagcg attatcatat ttatttatgt
      781 tactgtaagc catcatataa aagtaaatga gcagatcata gaaatataaa aaagaactaa
      841 aattgatcta aattatgtgc agcttgacta cgtaccaatc tatattactt actagattgg
      901 tcgttaatct attcggatcg aatgagcaaa atatttttgt gaatgtccat ccttgattat
      961 gattccaaaa agaatcattg ggaggctgtg gcgtaaatat tatactataa gccagaaata
     1021 cctatcattc tgaacttttt tatttaggaa ctttcatctc aaggcttttt caattaattt
     1081 gtacccctgt tttttggata agatcatgtg cattgatttt ttctatctct acgttcatct
     1141 cctacttaat gcctatctgg ctcctgaggg cttcttttta aggcgtcgtc atagtcttgg
     1201 ctgaggattt cttggtaatc tgagggaatg gcttcccaag tgagcaggcg aacgttcatg
     1261 acgtgttttc ctttgtgttt gattacagca tggtttttct ctttttctac ctctccagcg
     1321 tgaaaagaca gccatgtacc atggccaaag atatcattac atacccaacg ttcgatggaa
     1381 ttgtccacgt atcctacagg gagtgtggta tagccgattt gtggtttcaa aaaaggattg
     1441 aatcgatcga tcgtttcatt tttgtattga taattgataa gaaaaagata gaccaaacaa
     1501 aagacgagca gaaagaaaga tagtgtcttt ttgagtgaga agggttgttt tgatgaataa
     1561 gatttcttgt taacaagagg ttcatctggc aatgttggtt ctattgattc ggtagctgat
     1621 tcaatagaag gaaaatcaat cggcagacct aaagtatgat gtaaaagtga cagcatttca
     1681 aggtctggca ttgtacgaga attttcccaa ctagaaatcg ttttatcgga aacaccaatt
     1741 ttttcgccta attctttttg cgtaagctta caatttctgc ggtaagttcg tattttttct
     1801 ccggtgttca tcttcttctc ctttcgattc tacagtatat agatttattc tacatgacct
     1861 atacacttga tagatacgtt tttttgatga aaaacggtat gattttatca aagaatcatt
     1921 taagtaaggg agagtggtga taatcaaaaa aaagttcaca gttttactca caatcatagt
     1981 gaccggtatc atgagtggtt ttgccttggt tagtataaat aaacaggtac tctatcggac
     2041 aaatgaattt ttccatcaaa cagatactta tactccgatt cgattgccac aacattttat
     2101 tgggcaagaa gaatatgagc agctaatcat ggtatttgaa aatgtctcat cagaaactga
     2161 gctttcttat atgaaaaaaa cagtaaatga atccaatgaa gtagatacat cttggaactt
     2221 caagatacag ccaaagcttg aaatgacatt gtattatcaa ggggatgtca aatcttctca
     2281 cgagaaggta ccctattcta atacctcctt tcgcgtaacg tccattgaag aatcgatcaa
     2341 aataaaagat tttgaaggtg aatttttctt gatgacgaga gaaaaagaag cctatgaacg
     2401 gttcatttca ctattcactg atcgattcaa tcaagcattt cacactgatt atgccactaa
     2461 tatttttgaa gactttgaaa atagctattc gtattcggtt atttcaaata cagacgtata
     2521 taattatcga gtgtatttaa ctattggatt gattctttta ttccttttgt tatcatcctt
     2581 tttcttattg atgaataaag aaatcagttt attgttgaat aatgactttt cattacatca
     2641 agtattgtgg tgtttattgg ggaaaaagaa aatcattatt acattcataa ttgcaggaat
     2701 cattggtggt tttattttta tgtattataa ggagcaattt ctttatttgt tccagatgta
     2761 cgttttcttg ttcagcggaa tatatggtat ctctttgatt tctcttttat tagttgaaaa
     2821 aatcgcttcg ttaagagaag cgttcaacta tatccaatac attcttttta catttggatt
     2881 gttgttttct cttcttttga ccgttgccaa tgtcgaatta gcaactatcg ttttttcttc
     2941 ttcacagtta gctgtttccc atgaaccacc aaaagaaatc gctggtgagg actatcatgt
     3001 attttatcca gtaaccatcg ggaaaaacca cgtggatttt atttattcca atcgttttgc
     3061 atcagcagca gatcaggaat tatatgaaac tttgaataaa aacggaagta tattagtcaa
     3121 tgtatcagat tacttgaatg aagaaaatga acagtttggc agagggataa aaataaatct
     3181 gaattatctt aagaaatttc ctttgattga tgtctctgga aggttgatcc aaataaccga
     3241 aaaagaaacg caccctgtta tattgatccc tgaacgatat agacatacag atttaaaaaa
     3301 tgacctggcg caaataacag aatattatca agaaatcagt gaaaaagaac cgaaatttct
     3361 atttataaaa aacaatcaac ccatttatac atttattcct tctatcccct ggatcgaaca
     3421 ttatccggtt gttgaaatac tgacattgaa aaacagtatg tatttggaaa gaaatattct
     3481 cagcggagaa atctatccac cgttaaagat caagataggc tctttgtcga aagaaaggtt
     3541 atttgagctg attgaagaaa gtcagttacg tgataacttg cagttatcat ttccgtatac
     3601 tcagactgaa gagatagctg tcaaagtgtt aagcagatca tttcattact tgatacagat
     3661 atttttactc actttctttt ctttcttctt gttgagctat gtcatgttat gcatatattt
     3721 tgtctacaat tgtcggaaaa tcgctatctt tcgttcaagt ggttattcgc tctttgaaac
     3781 gtataaagat ttcttcatga tgaacctcat caaatgggga acgacaagtg taatcttcct
     3841 ttttcttata gaaagagagc ctaaatatct ttttaatatt tttttcttta gcttcattga
     3901 ttttattctt tcagttgttt ttattttgtc tacagaaaag aaaagtcagt tattgctgat
     3961 gaatggaggt taacaattgg agccgttgat agtgataaaa ggtgcgaaaa aaatcattcg
     4021 aaacaaaacg attcttcatt tggatcgttt cactgcggat gctggggatc ttgtgactgt
     4081 tgttggagaa agtggagcag gtaaaagtac gttattgaat atattaggat tgaatgatca
     4141 gttcagttca ggacaattct atttgttcga tcaagagacg aaacaattaa ctgggagaga
     4201 gaagcgcttg ataaaaagaa ataccatatc ttttcttttt caagattttg ggttgattga
     4261 atatgaaaca atcgatttta atttagatat tggattgaag ttttcgaatt taaatgtttc
     4321 agataagcga acagcaaagt atgaggcatt gaaaaaagta aatataaaca agaagctaag
     4381 tacgcctatc tacacattat ctggtggtga gaagcaaaga atcgcctgtg cgagagtctt
     4441 attgaagaat agcaaaatca ttttagccga tgaaccgact gggtcattgg ataaaagaaa
     4501 taaggataaa gtagttgaaa tcttatcgca acaagcttcc caaggtacgt tagttatcgt
     4561 agtcacgcat gatccatatc tgaccaaaat aggaaacaag aagatcatgc tttaaaaatc
     4621 aaaaaggaaa ggatgtcgtt ttatgaaaaa agcagttatt atttgtgcac tagggatagg
     4681 ttttgttgga ggaaacgcta tgtcatctga agtggaagca aattcagttc acggtgaaat
     4741 ctattacaaa tctttaaagc caggagagat tcattttgat gctaaaggta atattattaa
     4801 gtcaaattta ccagtagacc gggaaatttc tagaacagca attaatcata gctctggaag
     4861 atgggtttat tatagtgata caacttgtac gagaaaaaca ggtcattcta accactatag
     4921 tcgtgtctat aatcgtcacg gtgcaaaagc gaaagttgga aactctattc gatcagcaaa
     4981 tgcaacaaaa ggagtatggg cctatgcaag tgcgacagga aatagaactg acacattctc
     5041 atgttggtac aatccaaccg gttggtatta agtatccaaa agtttatata aaatagaaaa
     5101 aacatataat gaaacagcgt taaaagttat ttttggtagt tttaatatat tcatttttag
     5161 ttgtataaaa atatatcata ataaaaacaa caaaaaaatg tctggaaaat cgaacataaa
     5221 agtgtctgat ttgaatatcc cagcagcgga agaagaaaaa caaaatcgat aacatttgtt
     5281 ctaagaatta gttacgttaa atacggaacg caagatagac ttgaaaaacg gtaacaaaag
     5341 aagtagtata ttaaggtgtg atgtgctact tgacatttat tctgctgagc gaactttctg
     5401 gattgtgtag ccttatatct attcaggacg tatcgagcag attagtaaag gtgttcttga
     5461 tgattttcat cttgaacacc tttgttttta gcaattcgca aaaaacgaaa agtaggagaa
     5521 ttgataaatg aaaaaaagat ttttagcact tgccatcgta ctaggcactg gcttattgtc
     5581 aggctgtacg aatgccggtg aaaaaacagc cgttagttat aaaggcggta caatcagcga
     5641 acaagaagta atggatagct tgaaaaaaat gcaaggagca gattccgctg tgcaacagtt
     5701 gatcgtttat caagtatttg aagataaata tggagatgac gtatcaacca aagaaatcga
     5761 ttctcaatat gatcaaacaa agaaacaatt gggtgattca tttgattcac aattgaaatc
     5821 tgcaggttac acagaacaaa cattcaaaga cagtatcaaa caaagtctag cctttcaaga
     5881 aggtctaaag aaacatatca aattgaccga tgaagattta aaaacggctt gggaatcttt
     5941 ccatccagaa gtggaagctc aaattatcca agtagcaagt gaagatgatg caaaagatgt
     6001 gaagaaagct gctgataagg gagacgattt ctctaaatta gccaaagaca agtcaactga
     6061 tactacgaca aaagaagatg gcggaaaagt gaaatttgat tccactacta caacggttcc
     6121 ggcagaagtc aaagaagcag cctttaagtt gaaagatggt caagtttctg atgtgatcac
     6181 ttctaccaat gcttcaactt atactacgga atattatgtg gttaaaatgg tgaaaaacca
     6241 aaacaaaggc aacgacatgg ataaatacaa aaaagaattg aaagaaattg caacagacac
     6301 aaaattaagt gacagtacct tccagaataa agtaattggc gaagtattga aagacgctaa
     6361 tgtaaaaatc aaagacaaag actttgaaaa tgttttgagt acattcactt cagattctag
     6421 tacagcaagt agtaaataag cggtataaat aaaaaatatg aactatttaa atcaataaaa
     6481 ctgttcatct aaatcttttt
//
LOCUS       DAPCMU010000137         6401 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPCMU010000137 DAPCMU010000000
VERSION     DAPCMU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.141"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..177
                     /locus_tag="RD744_002320"
     CDS             <1..177
                     /locus_tag="RD744_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558301.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="RD744_002321"
     CDS             190..690
                     /locus_tag="RD744_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDU0558302.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="RD744_002322"
     CDS             787..2634
                     /locus_tag="RD744_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDU0558303.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="RD744_002323"
     CDS             2637..3533
                     /locus_tag="RD744_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0558304.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="RD744_002324"
     CDS             3583..3972
                     /locus_tag="RD744_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0558305.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..6304
                     /locus_tag="RD744_002325"
     CDS             3959..6304
                     /locus_tag="RD744_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0558306.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
BASE COUNT         2161 a          950 c         1370 g         1920 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacggga ttacgtccat
     6301 ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga gtacaatgtt
     6361 ttttgcagaa aacatactcg aataggactg atatcaatga c
//
LOCUS       DAPCMU010000138         6305 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPCMU010000138 DAPCMU010000000
VERSION     DAPCMU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.143"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..467)
                     /locus_tag="RD744_002326"
     CDS             complement(<1..467)
                     /locus_tag="RD744_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDU0558307.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANF"
     gene            complement(597..1307)
                     /gene="budA"
                     /locus_tag="RD744_002327"
     CDS             complement(597..1307)
                     /gene="budA"
                     /locus_tag="RD744_002327"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDU0558308.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1320..2984)
                     /gene="alsS"
                     /locus_tag="RD744_002328"
     CDS             complement(1320..2984)
                     /gene="alsS"
                     /locus_tag="RD744_002328"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDU0558309.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3192..3854)
                     /locus_tag="RD744_002329"
     CDS             complement(3192..3854)
                     /locus_tag="RD744_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDU0558310.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3864..4679)
                     /locus_tag="RD744_002330"
     CDS             complement(3864..4679)
                     /locus_tag="RD744_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDU0558311.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4940..5383)
                     /locus_tag="RD744_002331"
     CDS             complement(4940..5383)
                     /locus_tag="RD744_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDU0558312.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5517..5855)
                     /locus_tag="RD744_002332"
                     /pseudo
     CDS             complement(5517..5855)
                     /locus_tag="RD744_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5843..6220)
                     /locus_tag="RD744_002333"
     CDS             complement(5843..6220)
                     /locus_tag="RD744_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDU0558313.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
BASE COUNT         1908 a         1348 c          994 g         2055 t
ORIGIN      
        1 ttaaaatttg ctaataaaag atctacatat ttttctagaa atgcttgatc gatttcatca
       61 taccctttcg ttactccgca atcgatatct aaaacaccga ttaaattacc ttcttttacc
      121 attggcacta cgatttctga ttgtgcggct gaatcgcagg aaatgtaatt tgcatgggtt
      181 ttgacatcat caacgatcaa tgtttcttgt ttttcagctg attctccaca aactcctttt
      241 cccatcttga tacgtgtgca agaaactcgc ccttggaacg gtcctaaaat caattcatta
      301 ccatcgaata aataatatcc tgcaaagaca gtctcaggca aagtctcatt caacaatgca
      361 gatgaatttg ctaaatttgc taaccagtta tcttctgctt ctaataatcc ttgttgctgt
      421 aataataaaa tttcgtatgc atcttttttc tgtttttctg tccacatgat aaaaaccgct
      481 cctttttgat ctatggagaa gattatagct cagttttttt gtaaacgcta ggtttcctcc
      541 tcaaaaaaac gagatacgtc aggttaatca ccttgaacgc atctcgctgt ctttttctat
      601 tctgcttgtt ggatttctgc tgctaaatca gcataatcaa tcgtttcgtg aacaaaagtc
      661 ggatcatcaa ctggaaaatg ctgtacaagg gacgagatgc ggctaatctc aacttttcct
      721 tcagccactt caaaatccat tacatgtccg ccaaatgtat gtgtatcatc gataaaatgc
      781 aggtgaaatc ccgctgcagc aactccttgg aataattcag gcgtaaagaa cccgactaat
      841 gttccttgga tattgctttg ttggaattct ggttgagact ctgaaatttt tgctagtctt
      901 ctgtaaggtt tttcttgctt cggcatgata cggatgtgca tatttttgaa taatccagtt
      961 atcttcactg ccaaaaatag gttttccccg gcttctctta ctaatatgtc ttttttcaat
     1021 tgttctccat ctgcagtttt tgagacagaa aaactagtat ctggcgtaaa atctgtaatt
     1081 gctgcatagg gggtaagttc acttccatct aattgggcaa ctgagccatc tgatcttcct
     1141 tgataagcta cgccatctaa aaagatgact tcaccatcaa gcccatgcaa tgttcccaat
     1201 cccagagttc ctttttctaa tatatgagca atcgtttctg ttccatccat caagccggcc
     1261 atcaaagctc ctaatgtccc gtgctggtat aagatttttt cagtcatgtc gacacttcct
     1321 taatagaatt gatctggtaa gattgtttct cccaatttct cattatcacg gtaatcaatc
     1381 ggaatatcaa taattacggg tccttctgtc gcaaatcctt cttctaaagc tttttctaat
     1441 tcctctacac ttgtagctcg gattccttta gcgccaaaag cttctgcata tttcacaaaa
     1501 tcaactggac caaagtctac acctgacgcc cgctgatatt tcattttttc ttggaactct
     1561 accatattgt agtgtccatc attccaaatc aaatgaacga tattcaattt tttacgtacc
     1621 gccgtttcta aatcctgggc agagaataaa aatcccccat cgccggaaac tgatacgatt
     1681 tgtgtatttg gacgcaccaa tgcagcagaa atcgcccatg gcaatgcaac accaagcgtt
     1741 tgcataccat tactgaataa caaatgacga ggttcataac ttctaaagtg gcgtgccatc
     1801 caaatataat gactacctac atctacggta acagtcatat catctgttac ttttgactga
     1861 agcgtgttaa taacttctaa aggatgcaag atacctgctt ctccttttga ttgaacaatg
     1921 tcccgctccg ttaattttgc ttgaagagat gctaaatatt cttttgcatc ttctgacact
     1981 tgttgaggct ctaatgaacc tgtcaataaa tcaagagtgg ctgagatatc accgatcaat
     2041 tctctttcag gctgcataaa aggatcgatt tcagctggcg cttcatcgat cacaatgatc
     2101 cgcgcatctt tttccgcatt ccagtttctt gcttcatatt cgattggatc atagccaatt
     2161 gcaataacta gatcacttcg tttcaaaagc atatctcctg gttggtttct aaataaaccc
     2221 acacgaccaa agaaatgcgc ttctaattca cgtgagatca ctccagctgc ttggaatgtc
     2281 tctacaacag gcaatgctgt cttttcaact aattgcctga tggctaacgt ttctttttca
     2341 cttgatcctc gcatacctac taatagtacc ggtagttttg cttctctaat gcgttcagct
     2401 aggtaacgga tatcatctgc agaagcagaa cctaatttag gatcgctcaa tggcttgatg
     2461 acattacctt ggacaggtgc atccactaca tcttgcggga tactgataaa gctcgcgcct
     2521 ttttttgaac tcttagccat acgataagca ttggcaatca cttctgaaat tgtttctgga
     2581 tcttggattt ctgcactgta ttttgtgatt ggctggaaca gtgctgcatt atccatgctt
     2641 tgatgggtca acttcaatag atcgcttcgt tttacttgtc cggcaattgc taaaactggg
     2701 tcaccttctg ctgtcgctgt taccaaacct gtagctaagt tactagcacc aggaccactt
     2761 gttgcgataa ctactcctgg ctctcctgta atacgtccga ttgcttgtgc cataaatgca
     2821 gcattttgtt cgtgacgagt cacgattaat tcaggacctt gatcctctaa ttcattaaat
     2881 acaccatcga tttttgcacc tggtataccg aatacataat caacatgatg attaatcaaa
     2941 ctttctacta cagatttcga accttgtttc attttttcag ccattgaaca tcgtcctttt
     3001 tatgctttat ttttaattat tagttagaaa taatcacaaa taagtgatac agtttctaaa
     3061 cctgtttcat acaaaagtat agcacgtttt atttcatttg tttagccata tgtttcaaaa
     3121 aatgaaaaag aatgacagtt tttacataaa aaaaactgtc attcttaatt gaaagtttca
     3181 atttataaag tctacagatc catagttctc atcaatttac gattgttgaa gtaaatcaaa
     3241 ccaaatacga tagaaggacc tagtagaata caccattggg gctttacgaa aaagaagaga
     3301 aacaaacttg tcaatagtaa aaaatctagt aaactcacat accatttttt caacgtatag
     3361 tagaacgcta tccgtagaac ttgatttggt ttcgacgctg gatttctcac ctgaaaatac
     3421 cagcagttgg tgctaaagga aactgataaa caagcaagta aaacaaacaa cggaatcaac
     3481 cattttccat aagaaaaacg tataaaaaag aaacaatcaa aaatcattat cacactaact
     3541 atccaagcaa atagccaata aaaaaatccc tttagccaaa attggcgaaa catctttatg
     3601 aacaaacgta ctggtgaaaa actattttct tttgctaatt cttctgtcat acctatcatc
     3661 acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa aggaaaagac attccttcca
     3721 tctatcgaca aaaggaacgc agctgctata tacaagatat tgacacacca aaaacaaagg
     3781 ctacttgcca atgattgata caaccaataa aagaattcca tataaacatt tttgtcaaaa
     3841 cgctgctctt tcataatcac ctcctattcc ttcattcctg atgaagcgat cccttctaca
     3901 aaatattttt gcatcgctaa aaatataaaa gcaacaggaa gaacagaaac aactgaggca
     3961 gccatgatta atgcgtactc tgcatcaaat agcccaataa acattttcaa ccctaactgg
     4021 attgttttat tttccgtaga agacaaataa atgaatggcc ccatataatc gttccatgta
     4081 ttaacgaatg tgatgatcgt caaactggca atcgccggtt ttgtaagagg taaaatgatc
     4141 ttgcgataga ttccccactc tgataagcca tcgatccgcg cactctcaca taaagaatct
     4201 gggatactac tgtaatattg cttcaacaag aacacaccaa aagcattgaa cgcttgtaat
     4261 agcaccaacg accacaacgt atctgttaaa cccacttttc tcatcataat gaactgcggg
     4321 atcatataag actgccaagg gacagcaatc gtgccgatat atgctaaaaa taaaatatcg
     4381 cgccctttaa aatttagttt agaaaacccg taagcagcaa aactagaagt aaacaactga
     4441 atcaacgtga tcacaacgct taaaaaagct gtattcttaa aaaatgtcac taaaggaata
     4501 cgagaccaaa taaccagata attctctaaa tgccaagttt tagggatcca ttgaatcgga
     4561 atcgtgaaca cttcattatt tgctttgaat gaagcagaaa ccatccataa aaaaggaatg
     4621 atcgtcaata aagctaatac aatcagcaga atcataccca accattgaat aaagggcatg
     4681 ttttttgaac gaatttttgc tttttttctt tcatttcgaa cacctccaaa gttataatct
     4741 tcatttcttt gtaatgatta caatccagaa caacggttat tatagctgat tcatttccgt
     4801 tcttcttatt tttgtaagcg cttatttaac aagttatgta aatctcttta tttttttgat
     4861 ttaaaaatga aaaaatacaa aagcaagact gtcatacgtc tcgcttttgt attgaaattt
     4921 cttcgattag agatacttat tactgttcaa aagcttcttt gaaacggtga atcaattcat
     4981 tgactacagg tgataaaatc cgattctttt tccagactag catgcagttc gtttcaatca
     5041 ttgggtcaag cggaacgaat gtcactcgct ttgaatgatg acttacagca ccttctatga
     5101 ctaaagcagc acctacctga ttctcaatta gcgcaaatac attgaaaatc aaattgtacg
     5161 ttcctgtgat cagcaactgc tcctttttta tgcctaacca gtcagcaaac attcgctgta
     5221 tttctggacg ctttgagatc aaaagaggta cattcgcaaa gtcttctttt gttatcacgt
     5281 ctttttttgc taaaacagaa ttttctaaaa tgagcaatcc ccatttttcc tttcttggta
     5341 acaaaatcgt ttcgtatttc tctgtatcta ctggatctag caaaatcgct aaatctaaca
     5401 ggcttttttc tagtcactcc gtgatatcat cacttgttcc gctgtggata tgaaattgaa
     5461 cgctgggata atcacggatc aacttttcta gtaatagtgc caatgtattc gaactatcag
     5521 cttccacaca gccgatcgta aatcggccgc tgaataattg tttttttctg ttctcgaatt
     5581 cttgagtggt ttgattcgtt aaatctaaaa tttcttctgc tcggcttttt tagaaaataa
     5641 cctgcttctg tcaagatcat tttgcggttt tcgcgtacaa acagttctgt tcctagttac
     5701 tcttctaact ccttcagttg tcggcttaag gttggttgtg taagatgtag aacttctgcg
     5761 gcttgagaga ttgttccttc ttctgcaatt gtccaaaaat attttagtaa gcgtaactcc
     5821 atagcgatgc tccttgattt ttttacttga tacattccat tttatttcta gataacacac
     5881 gatcatattt cttgattttt ccttgtaaac gttccaaaca ttcttgcatt tcagcaattt
     5941 ttttcgtcaa aagttcactt tcttcaatca aaagcttacg ccgctcaaat acagttgctt
     6001 ctccttcgct agataggcga atatattcaa tcaaagactc gatcgataat ccggcatttc
     6061 gcatacagat cgtaaagtcg acccatttta ggtcttcttc tgtataatca cggattccgc
     6121 cttttgtccg attaactggt ggaatcaacc cgattcgttc ataatagcgc aatgtatcgg
     6181 ccgaaatgcc cttttcttca ctgacttgtt tgatattcat ctgatcactc tttctcttaa
     6241 tttcttgcca ctttacttat gtattttatt tattctacaa ctttaagtgt actccaagtc
     6301 aatac
//
LOCUS       DAPCMU010000139         6303 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPCMU010000139 DAPCMU010000000
VERSION     DAPCMU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.144"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            334..714
                     /locus_tag="RD744_002334"
     CDS             334..714
                     /locus_tag="RD744_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDU0558314.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="RD744_002335"
     CDS             696..1610
                     /locus_tag="RD744_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDU0558315.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="RD744_002336"
     CDS             1855..2544
                     /locus_tag="RD744_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558316.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="RD744_002337"
     CDS             complement(2665..2751)
                     /locus_tag="RD744_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDU0558317.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="RD744_002338"
     CDS             3427..4377
                     /locus_tag="RD744_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDU0558318.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="RD744_002339"
     CDS             4398..4931
                     /locus_tag="RD744_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDU0558319.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="RD744_002340"
     CDS             4947..5117
                     /locus_tag="RD744_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558320.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="RD744_002341"
     CDS             5567..6100
                     /locus_tag="RD744_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558321.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAPCMU010000140         6203 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPCMU010000140 DAPCMU010000000
VERSION     DAPCMU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.145"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            257..532
                     /locus_tag="RD744_002342"
     CDS             257..532
                     /locus_tag="RD744_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558322.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="RD744_002343"
     CDS             545..2617
                     /locus_tag="RD744_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDU0558323.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="RD744_002344"
     CDS             2782..4668
                     /locus_tag="RD744_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDU0558324.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="RD744_002345"
     CDS             4680..5627
                     /locus_tag="RD744_002345"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDU0558325.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="RD744_002346"
     CDS             5647..6174
                     /locus_tag="RD744_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDU0558326.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT         2051 a         1015 c         1358 g         1779 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta ttt
//
LOCUS       DAPCMU010000141         6173 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPCMU010000141 DAPCMU010000000
VERSION     DAPCMU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.146"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            725..811
                     /locus_tag="RD744_002347"
     CDS             725..811
                     /locus_tag="RD744_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDU0558327.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="RD744_002348"
     CDS             complement(932..1621)
                     /locus_tag="RD744_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558328.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="RD744_002349"
     CDS             complement(1628..1906)
                     /locus_tag="RD744_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558329.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="RD744_002350"
     CDS             complement(1866..2780)
                     /locus_tag="RD744_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDU0558330.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="RD744_002351"
     CDS             complement(2762..3142)
                     /locus_tag="RD744_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDU0558331.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="RD744_002352"
     CDS             complement(3592..3879)
                     /locus_tag="RD744_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDU0558332.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="RD744_002353"
     CDS             complement(3899..4123)
                     /locus_tag="RD744_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDU0558333.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="RD744_002354"
     CDS             complement(4578..4793)
                     /locus_tag="RD744_002354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558334.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="RD744_002355"
     CDS             complement(4763..5296)
                     /locus_tag="RD744_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDU0558335.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="RD744_002356"
     CDS             complement(5317..6159)
                     /locus_tag="RD744_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558336.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1634 a         1151 c         1041 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtatatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAPCMU010000142         6002 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPCMU010000142 DAPCMU010000000
VERSION     DAPCMU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.147"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..395
                     /locus_tag="RD744_002357"
     CDS             <1..395
                     /locus_tag="RD744_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558337.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="RD744_002358"
     CDS             398..1228
                     /locus_tag="RD744_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDU0558338.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="RD744_002359"
     CDS             1244..2095
                     /locus_tag="RD744_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDU0558339.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="RD744_002360"
     CDS             2092..2451
                     /locus_tag="RD744_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDU0558340.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="RD744_002361"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="RD744_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="RD744_002362"
     CDS             2624..2929
                     /locus_tag="RD744_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558341.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="RD744_002363"
     CDS             2929..3240
                     /locus_tag="RD744_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDU0558342.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="RD744_002364"
     CDS             3237..3410
                     /locus_tag="RD744_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558343.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="RD744_002365"
     CDS             3407..3823
                     /locus_tag="RD744_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDU0558344.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="RD744_002366"
     CDS             3823..4491
                     /locus_tag="RD744_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDU0558345.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="RD744_002367"
     CDS             4488..4865
                     /locus_tag="RD744_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558346.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="RD744_002368"
     CDS             4862..5161
                     /locus_tag="RD744_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558347.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="RD744_002369"
     CDS             5151..5309
                     /locus_tag="RD744_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558348.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="RD744_002370"
     CDS             5403..5819
                     /locus_tag="RD744_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDU0558349.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAPCMU010000143         5941 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPCMU010000143 DAPCMU010000000
VERSION     DAPCMU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.148"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(167..625)
                     /locus_tag="RD744_002371"
     CDS             complement(167..625)
                     /locus_tag="RD744_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0558350.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(638..1969)
                     /locus_tag="RD744_002372"
     CDS             complement(638..1969)
                     /locus_tag="RD744_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDU0558351.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1980..3422)
                     /locus_tag="RD744_002373"
     CDS             complement(1980..3422)
                     /locus_tag="RD744_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0558352.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3409..4173)
                     /locus_tag="RD744_002374"
     CDS             complement(3409..4173)
                     /locus_tag="RD744_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDU0558353.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4277..5152)
                     /locus_tag="RD744_002375"
     CDS             complement(4277..5152)
                     /locus_tag="RD744_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0558354.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5407..5491)
                     /locus_tag="RD744_002376"
     tRNA            complement(5407..5491)
                     /locus_tag="RD744_002376"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5455..5457),aa:Leu,seq:caa)
     gene            complement(5509..5580)
                     /locus_tag="RD744_002377"
     tRNA            complement(5509..5580)
                     /locus_tag="RD744_002377"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5546..5548),aa:Gln,seq:ttg)
     gene            complement(5590..5662)
                     /locus_tag="RD744_002378"
     tRNA            complement(5590..5662)
                     /locus_tag="RD744_002378"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5627..5629),aa:His,seq:gtg)
     gene            complement(5724..5808)
                     /locus_tag="RD744_002379"
     tRNA            complement(5724..5808)
                     /locus_tag="RD744_002379"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5772..5774),aa:Leu,seq:caa)
     gene            complement(5829..5899)
                     /locus_tag="RD744_002380"
     tRNA            complement(5829..5899)
                     /locus_tag="RD744_002380"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5865..5867),aa:Cys,seq:gca)
BASE COUNT         1824 a         1260 c          903 g         1954 t
ORIGIN      
        1 tttaagaatc ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt
       61 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
      121 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      181 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      241 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      301 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      361 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      421 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      481 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      541 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      601 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      661 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      721 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      781 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      841 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      901 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      961 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
     1021 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1081 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1141 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1201 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1261 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1321 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1381 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1441 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1501 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1561 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1621 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1681 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
     1741 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1801 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1861 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1921 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1981 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     2041 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2101 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2161 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2221 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2281 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2341 acgtaataca aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2401 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2461 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2521 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2581 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2641 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2701 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2761 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2821 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2881 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2941 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     3001 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3061 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3121 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3181 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3241 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3301 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3361 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3421 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3481 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3541 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3601 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3661 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3721 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3781 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3841 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3901 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3961 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     4021 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4081 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4141 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4201 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4261 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4321 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4381 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4441 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4501 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4561 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4621 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4681 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4741 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4801 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4861 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4921 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4981 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     5041 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5101 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5161 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5221 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5281 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5341 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5401 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5461 ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg
     5521 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     5581 agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5641 ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac
     5701 taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg
     5761 ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat
     5821 tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt
     5881 accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata
     5941 g
//
LOCUS       DAPCMU010000144         5936 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPCMU010000144 DAPCMU010000000
VERSION     DAPCMU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.149"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(16..1014)
                     /locus_tag="RD744_002381"
     CDS             complement(16..1014)
                     /locus_tag="RD744_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558355.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            1212..2453
                     /gene="sstT"
                     /locus_tag="RD744_002382"
     CDS             1212..2453
                     /gene="sstT"
                     /locus_tag="RD744_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDU0558356.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2600..3235
                     /locus_tag="RD744_002383"
     CDS             2600..3235
                     /locus_tag="RD744_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDU0558357.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            3426..4166
                     /gene="truA"
                     /locus_tag="RD744_002384"
     CDS             3426..4166
                     /gene="truA"
                     /locus_tag="RD744_002384"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDU0558358.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(4311..5909)
                     /locus_tag="RD744_002385"
     CDS             complement(4311..5909)
                     /locus_tag="RD744_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558359.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2010 a          897 c         1116 g         1913 t
ORIGIN      
        1 cactgctaat aaatatcaaa taaaaaactg cccgatattt atcagccatg ccttccctga
       61 gtttggaaat tcacgtttca taaatgtaga tacgcgataa gaaactaaag ggaaattaca
      121 tataacttga gtgacaatca aaaatacagc aagaaaattt cctcttcctt ttatacctaa
      181 tttatcagat agcatttcta aaaataaaaa cgaagagaaa gtataataca ttgcgaagag
      241 agaatatggt ttagaaaacc tcagcgctgt atctgtattt cctctgatga tgagtgaaat
      301 ttgtgtcatc aaaataatca aaccgaataa aacaatcaag ctgataaata cttgaggaag
      361 cttatagcgg accttttctt tccagttcca ttctttcaaa ccataaccca ttaaaaataa
      421 ataaaaaatc cataaaatac tctgtccatt ctgtaatgca aacgggtctt tattaaatag
      481 agtagagcag ccaactaatc caattgttaa aaataatagt agatatttaa ttgttgtttt
      541 cgataattta tttaaaaatc caactgtata aggaatacta caaaaaagca aagtacaaga
      601 aacagcataa gtaaagtaat tctgactgat aggtgttagg ataagccaat aatcacgaat
      661 attcaagctg ccagcttgta cgtaaaaaaa gatattgcaa actagtgtgg ctattaggaa
      721 tataaaggca aaacgactta aataatgtaa aacttttaaa cgggcgattt tttgatattg
      781 gtaacccatg taaagtggga tcaaatttgc accaataaca acggaagttt gtaaaaatgt
      841 atagatgtag aatcgaccag ggtttgttgc atatgtagga atagtgactg ctgaaatcaa
      901 tatacgctgt aaaaccatca ttatacaagc gacaaagatt gtcaggaagc cataactctc
      961 cttctttatt tttttcaaga ataaacacct cacaaaaaaa ttggacaaaa acataagaaa
     1021 acgtttcctg ataagttgcc cattaataac atcctaactt agaatatctc aaaaaacaac
     1081 gtgtagacag aaaaagtaca aaattagcaa gaaatggttg tttgtattgt tagaattttt
     1141 agaaaattat acctttagtt tctttgcata aaatgataca atgattgtta aaattgtgaa
     1201 agggtggtca tttgttagag aagtttttgg atatgtcgtt agttcaacgt attgttgttg
     1261 gtattttagt cggaatattg tttggtatct ttttccccac atggacattt atttcaatat
     1321 tgggaacgtt gtttgtcagt tgcttgaagg caatcgcacc tattctggtt tttttcttga
     1381 cgatggcgtc aattgccaag cataaaattg ggaataagac attcgtgaag cctattttga
     1441 tcttatattt agtgggaaca ttgctttctg cacttgtagc agttattgta agttcaattt
     1501 ttactgttcc tattacttta caagaaacag tatctgaaaa agcaccgcaa agtttggaaa
     1561 gtgttttatc tactatgttg acgaatgtca cacaaaatcc tgttcaatct cttattgatg
     1621 cgaattacct tggcgtctta ttctgggctg tattgttggg actagctttt cgagcacgtt
     1681 cagaatcgac aaaagagttg gtggatcaga tatctatagc gttgtcacaa gtcgtacaag
     1741 tgattattgc atttgctccg atagggattt taggattggt ttatcaatca attgctacga
     1801 ctggaatcgc tggattggca gagtatctgc agcttttact tgttttgatc ggtacgatgc
     1861 tgtttgtcgc attagttgtg tacccattcc tcacgttttt gtttattaaa gaaaatccct
     1921 atccattgat tttcttttgt ttgaaagaaa gcgcacttcc cgctttcttt acccgaagtt
     1981 ctgccgcaaa tatcccgata aacatgatgt tggctgaaag attgaaattg acaaaagagt
     2041 cttattccat atcgatcccg ttaggtgcta cgatcaatat gggcggtgcc gcgattacca
     2101 tttccttgat gacgttgact gctgtacatt ctttagggat tgaagcacct tttgttttga
     2161 aagtcctttt atgtattttg tctgctttag ctgcttgtgg agcttcaggt gttgcgggcg
     2221 gttcactgtt attgattcca ttagcatgta gtttgtttgg tatctctaat gatgtcgcga
     2281 tgcaaatcgt cggtattgga tttattatcg gtgttatcca ggattcaatg gaaactgcct
     2341 taaattcgtc aagtgatctg ttgtttactg caataggaga gctaggcgcg cgtaaaagaa
     2401 gtggggaaaa aatcaaattg aaagattgtt tagatggatt aagtgaacgt tgaacgttag
     2461 atatttagta tttttaaaat gattcgagtt tttaatgagt tatatagatg ttcgtgtaaa
     2521 tagaggtttc tgttaaatca agataaagtc gtttataata acaattaaaa gcataggtaa
     2581 ttgttattgg aggcgtatca tggatttttt acgtaaattt ggttttttct ttttattatt
     2641 aggcgtgctg agtgattttt tgactccgta cgtattagga ttgtattatc ctgaattaga
     2701 tcaaatgaag agcgtgatca gtgtatttgg agaggtttcc agtcctgtaa gaaaagcgtt
     2761 tcttatttgg tctgtggttt cagggatctt tttcatcctg tcattgcctg cattgtatgc
     2821 tgcatttgtt agtacatctc gtgtgttagc taccctagtt acagcagcca tcgcgttcta
     2881 tggtataggt gattgtatct ttacaggttt gttcagtatc aatacccatg aatcttcgtg
     2941 gaatgtctcg acatgggtcc ataatatcgg atcaggttta ggttacagtg gttttttatt
     3001 gtttccagtt cttcttttct tgatctatcg aaaaaatggg caaatggacg taagccactt
     3061 ataccttgga ctgtctgtga tcagtttgct ttttgcggcg atttatggac tggcaagaat
     3121 tccttctata aattcttggc cattactgaa taaaataggt ttttgccaaa gaatcagttt
     3181 tttctttaat tatgtgccca ttgccaggtt gagtatcttg catctcaaac agtaaaactt
     3241 gagccgtcaa attgaacagt cagcggatac ttcatccggt ggctgttttt tgtttttctg
     3301 aaatatatat ttccacagtt ttcttgtata gcttctgtct tagagaagca aggagctata
     3361 ttttgtgcta caatcttaaa aaggtagaga gaagaccaaa tggaagacag aggagccact
     3421 aaaatatgag aaacatcaag ttgacaatcg aatatgacgg aaaaagatat tcaggatggc
     3481 aaagacttgg agatgacgat aaaaccattc aaggaaaaat cgaaaaagta ttgcatcaga
     3541 tgacaaatga agagatagaa attattggat caggaagaac agatgctggc acccatgcgc
     3601 gaggacaagt agcaaatttt aaaacaaaca cagaaattgg actggcagaa atgatcgact
     3661 ttatgaatcg ttaccttccg cgggatattg tcatcaaaag aatcgaagaa atgcctgagc
     3721 gcttccattc aagatataat gcggtgggca aaaaatacag ctattatgtt tggaacaacg
     3781 tgattccttc agcatttgag cgaaaccaca gcttttattt ccctcaagag ttggatatgg
     3841 acaagctgaa tgcagcttgc gaaaaattag tgggtacaca tgatttcatt ggtttttctg
     3901 cattgaaaaa atcaaaaaaa tccacagtgc gtacgattga tgaattatct atcgaacgaa
     3961 agggagaaat gcttcatttt acttttgtcg gagacggatt cttatataaa atggtgcgga
     4021 ttatcatggg gacattacta gaaatcggca caggtgccat gccgatagaa acaatcgatg
     4081 aaatatttga aagcaaagtg cggcaacatg caggagaaac cgtccctgca caaggtctgt
     4141 ttttggatga agtttattat cgataaaaat actttgataa tggatgaaac gcatgtttca
     4201 cagtataaat atcgatgtta aataatgtat gaagcaaatt aaagaaatat aaaaaaagac
     4261 tttttagcct gtttatgtgt acagctaaaa agtctttctt aaataaaaat ctagtgagaa
     4321 tgttgtactt ctcttctcgg ttgtttttgt ttagaaaaag taaatcgata aagtaaaaat
     4381 aaaatcaaag taacgataga taaatatctt gaagcaattg ctagttttgt gtactgataa
     4441 gtaataacga ctttatttat acctggagga atagttaatt cagttgtgcc aaacttagat
     4501 aattttgttt gtactagttt gccattaata gaagccactt gccctttata acgataaata
     4561 ggtgtcgtca agacagtatt tttattattc gaattatcta attcaattgt aagtttgtta
     4621 ctcataaaat gagttgttgg atttatttcc tggttaccta gtaaatattg atcattatta
     4681 atcatatcag gatgatagat tgactctttg tttgcataat cagtatgatt gtaattttca
     4741 gctatagcaa cagcgtcttc aggtgctata agtgttaatg aatctttgtt agcatggata
     4801 gaattgctta ctcctgagag atgcaatatt agtgcaagaa acgtaaagac cattattttc
     4861 catgaattat ttttagtaga aaaaatataa tagatcacta tactcatcgt ataggctata
     4921 aataaagaag aaaaaccatt taaccgccag acaaattgta gactcttaac tggtgtatgg
     4981 ttaaaaagtt gccaaggaaa taaattggtt gaacaaaaaa gaacaaatac accaagaaag
     5041 aaaataaata aatcatcagc agttaatttt tttataaaaa taaaagcacc aatcaatccg
     5101 agaaataaga ataatccaat agtatagttg ttatagttat tatttaaaat atgtgtcagc
     5161 atatcactag gagccatacc acttaattct tttcccagtg ggactttcaa ttcttgtgct
     5221 ctagattgtt gaataaaagg aattaagaat cccgcactta aaaaaaaggt gaggacggta
     5281 gcttttaaaa gacttaagag tctagcgatt ttttggtccc aaaaataaaa agaaagaaac
     5341 aacgttcctc caataaatac agaaaccata gcgacagaaa gtaaatgagt atacactact
     5401 agcgtcatgc caatagacaa ccagtaccat ttttggtaat ctctaatata aatttcgtaa
     5461 caccccatta gtatcaaagg caaaaatgtt aaagcgacag cttctcccaa tgaagcacga
     5521 tggaatatgt caatggcacg ataggcggaa aatgtataaa taatcgaaaa taacagcgaa
     5581 atataacgat tgttttttat ttgtttcata gaaaaataag aaacgaccat tgttaaaaaa
     5641 gtgataaaaa aatagtagat attataactt aaaacaagat ttcccatcat tttataaaat
     5701 aaaaaagctg gataaagagt aagccatgga taaaaaatat tcatcattgt tccatgatga
     5761 tcgaagttat tgaagtttac tggagaactc catacattat ctagtcctat gattcgagat
     5821 aaatgaaagt aagtatcctg attgacacta tgatagattc ccttagacat gaatagaggg
     5881 atgacgtaag caaaacttag aaataacata aaaatgatat ctattatttg gttttt
//
LOCUS       DAPCMU010000145         5894 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPCMU010000145 DAPCMU010000000
VERSION     DAPCMU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.150"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(170..3319)
                     /locus_tag="RD744_002386"
     CDS             complement(170..3319)
                     /locus_tag="RD744_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDU0558360.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="RD744_002387"
     CDS             complement(3333..4550)
                     /locus_tag="RD744_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDU0558361.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5894)
                     /locus_tag="RD744_002388"
     CDS             complement(4540..>5894)
                     /locus_tag="RD744_002388"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDU0558362.1"
                     /translation="ATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLAST
                     YNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLIS
                     QFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLT
                     HPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVV
                     MNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHG
                     VLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDA
                     SREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDT
                     FEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ
                     "
BASE COUNT         1613 a         1253 c          987 g         2041 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaa
//
LOCUS       DAPCMU010000146         5850 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPCMU010000146 DAPCMU010000000
VERSION     DAPCMU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.151"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(249..1031)
                     /locus_tag="RD744_002389"
     CDS             complement(249..1031)
                     /locus_tag="RD744_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558363.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1096..1932)
                     /locus_tag="RD744_002390"
     CDS             complement(1096..1932)
                     /locus_tag="RD744_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0558364.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1950..3359)
                     /locus_tag="RD744_002391"
     CDS             complement(1950..3359)
                     /locus_tag="RD744_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0558365.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3373..5196)
                     /locus_tag="RD744_002392"
     CDS             complement(3373..5196)
                     /locus_tag="RD744_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0558366.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5476..5703)
                     /locus_tag="RD744_002393"
     CDS             complement(5476..5703)
                     /locus_tag="RD744_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558367.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1801 a         1270 c          950 g         1829 t
ORIGIN      
        1 gtagtataaa atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag
       61 tctaaaacac ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa
      121 taaatatagt cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac
      181 tggagatgta taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg
      241 aagaatcatt attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc
      301 ttaaatacca cagaataatc ttttttccca ttttcaagta accgaagtgg aatgtcttta
      361 ccaacttgct ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa
      421 gattgagcat ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt
      481 acaagcttcc aagtaggtgg tatagctgtg tatgtttcga tgaatgaatc aattctttca
      541 tctatcgtct tcccagtatc tacttctgtc tcattactct tagatgaatc gattctttca
      601 tccatcgtct gctccgtaac tgcttctgtc tcattattct caccggaaac tctcttcaca
      661 cccaaactta tatattgtcc atctttagtg tgcatagtta aaattcccac gcttttacta
      721 ataaattcac tataaccagt tctttcaata gagacttcaa tgtatttcac actatcatac
      781 tcaaaagaaa aatagacatg taaaagcatt ttgttgttta catctacata ataatccttt
      841 gactctactt gagaccttcc tatacggaat acagctttac tgtctgatat ctctatacca
      901 gcatccttat tattaatctg ccaatctcca agtagctctt ctggcaagct agtagtttct
      961 tgtgaacact ttaccacttc actggtagag gtcgaaacat tcacctccgc actgttagaa
     1021 gaattttcca aggaatcagg agccttagca caaccaacaa gtctttatat attgtgcagc
     1081 cgatattttc ttcattcagt acacccttga aatatgcagt gttagataaa gcagttcgtc
     1141 ctgatttgct gaggcaccca tctcttcttg aacgtactcc ttgattttca aggccgcccg
     1201 atactctttc ggaaaacgct gcttcactga agccagcaaa aaatcatcct tctccggcaa
     1261 ctcttccttc ttaatcattc gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta
     1321 aagcatcgaa tctttatcaa cctttttttc gggaaacaga tatcctagca tagccaagat
     1381 atcctttagt gctcttaccg agagcatggt ttcttccata tttgcttgtt cactttgggc
     1441 atttacaatg tgaaacgcaa tgtttccagc ctcctcttct ggcaactgaa cattcatttt
     1501 tttgttgatc aattggatag catagcagcc gatctcaaat tcttttgggt aatacttttg
     1561 aatttcaatc agcaggcgat tttgaaaaac aatgcctttt tgactgcgtt caatggcatg
     1621 cttcaagtga tcaaccaagg taaaaaatag acttttattc agcttaaagt caaacttggc
     1681 gttggcataa tcgataattc gcttgacgca aataattaga tcatccggag cagcctcaaa
     1741 cagtttaatg taatcctttt ttagagcatc agaaagtgaa tctaatttaa atactttttg
     1801 gattttgtca gccggaacca aatcgccttt ctttagttga aatccaatcc ctttccccat
     1861 caaaattact tctttgcctt gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt
     1921 aattactctc attagattca ccgttctctc taagctaagc catttcttgc gattactttt
     1981 tgataccaaa agaagctttc tttttttatt cgttttaatt ctttaagatt ctcatcggtt
     2041 cggtcaacat atataaaacc ataacgctta ttgaagcctt gatgggtact aacaacatca
     2101 atcgctgacc aaggtaaata accaaaaaca tctacaccat ctgttaaagc tgcttgaata
     2161 gccagaatat gtttggctag ataatcaatt cgatcctggt cattaattcg tctatcttcc
     2221 tcaactatgt cgggtgcccc atagccattt tcagtaatta aaataggtaa atcatagcga
     2281 tcgtacaatt tcctcaatgt taatcgtagt ccagtagggt caactaccca gccatatggc
     2341 gttttatcta cataaggatt ttcagctgcc cggtagacac cttcttctcc caacatgatc
     2401 tgttgatccc ccgctctagc ggaaacatct gatccgtcat ttttgctggc agcgatggtg
     2461 gcagttgaat aataattaat cgcaataaaa tcgggtttac cggaggataa aattgcctga
     2521 tcctctgcct caattttagg atataatcca cgatcattca aatagttttc aaacaaataa
     2581 ttgtattttc cccgaactgc tacatctaag aaagaccagc ctctcaatgt ttcccaatta
     2641 tgggctgcaa ttgcatcttc tggactggag gtagcttgat acatagccgt taaattgatt
     2701 gctggaccga tttttccttt aagattcaac tgatgataca agtgaattac tctagcttga
     2761 gctaataaaa catggtgatt aatttgaaag agttcttttt ttgaaggcag ctctccactt
     2821 ggtggcaaac caatcgctcc aggatgaagg atcatcgtgt tttgctcatt aattgtcagc
     2881 caatagttca ccttgtcacc atactcagta agcaatacct gtacatagtc cacaaaataa
     2941 tcgatagttg ctcgatttaa ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa
     3001 tggtacatgg tgacaatcgg ttcgattccc tgattcaggc attcatcaat tacttgatga
     3061 taaaaagaaa ttcccttttg attggtttta ttattttcaa tatccataat tcgcgtccaa
     3121 gcgatggaaa agcgaaaagc ttttaagcct aattctttaa acagctgtat atcttcttta
     3181 tagtgatggt aaaaatccga ggcaacttca aaattagcta ccccttctgt atgctgatac
     3241 ttgtcaataa tagacggttg tttcccatcc tcctgatagg caccctctgc ctggtaggca
     3301 ctaatggagg caccccataa aaacttctct ggaaatgctg aaagctgttt gtattgcaca
     3361 aaaatcctct ccttaactaa tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc
     3421 ttcactaatt cctgcgtaat tttcactgtt ggtcactacc acaatcactg tatcatcgta
     3481 gccagcgttt ctaatggcag cgaaatcggc gttgattaat aattgtcctg ctgacacctg
     3541 atctcctgct tttacaaata cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc
     3601 cacatggatc aataattcaa taccttcttt agaacgtaga ccaatcgcat gtttagattc
     3661 taaaatagta atgacttctc cctcaacagg tgaataaatg ctgttttctt ctggatgaat
     3721 ggctacgccc tttcccaata gtcccttaga aaagacagca tcgttcactt cagaaatagg
     3781 acacacggtt ccttttaccg gattttcaac aatgactgct tcggctattg ccgtttgctc
     3841 tgattcatca gaagtatcgt caaatccaat tagataagca atgataaagg ttgcaacaaa
     3901 ggcgattaca cacgtaataa ttgcgtgaac aatattcatt ggattttctc caataaaggc
     3961 tggtaaggct gctaagccag gggaaacaaa ggcatatctg actaaaccgc tcaagccagc
     4021 atacaaacca gcaattgcac caccaatcat ggaagcaact aatggttttt tcagcttcaa
     4081 ggttactcca tagagtgaag gctcagtaat tcccattaaa gcagtgatcc cagtggacac
     4141 agctagctgc ttcaactctt tgtttttagt tttcgcggct acagcaaagg tagcacctga
     4201 ttgagcaata ttagaagcca gcattcctgg cccattgatc atttcagaac ccgtactgct
     4261 caattgcata gtagcgattg gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg
     4321 ctgcaaacca cccatcaaaa atggaattaa ccaagctgct ttgccgttaa tccattcagc
     4381 ccccattgct ataagatcgt tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa
     4441 aggccccata attaaaaata ccagcatcgg cttagtaaag aatttaataa acgaaggcga
     4501 aattcgatcc gcaaagggct ctacatagga catgatccaa acagtaaaga taatcggtaa
     4561 aactgatccc gcataatctg cagctaatac cggaattcct agaaaagaaa cggcatcgcc
     4621 agtagaaaaa agctgtgaaa tatttggatg aagcaaaatg ccagcaacag taatcgccaa
     4681 aattgggttg acatcaaact ttatggctgc cccataggct aatagtactg gcataaaata
     4741 aaaggctgca tcagcaatag tattcaaaat gatgtaagtt tgactactgt ctgatagtac
     4801 accagtcaaa gtcaagacgg ccaaaatggc ttttaccatc ccggcacctg ttatagctgg
     4861 aatcatcggt gtaaaggtgg tagaaatgac actaatcaat cttgaaataa tactttcttg
     4921 attgtctgaa gaggggccct ttttaattga agttcccact tccttttgaa tttccttgaa
     4981 ggctgtttgg acttcattgc caatcaccac ttggtattgt ccaccgctat ccaccacact
     5041 aattacacca agtgtttttt ctaaggcttt cgtatcggct ttccttttat ctttgagttc
     5101 aaaacgcagt ctggtggcac aatgggttaa gctgaccaca ttttcttttc ccccaacctg
     5161 ttgcaataca tttcttccta cttctctgta atccattatt ctctctcctt aaagcagtaa
     5221 aaaagaccaa gtcaaaccac gccccaatca acattgaagt gttagtttgt cttggtcttg
     5281 cctgcataat cagtaacatg ccaataacgt tattttgctg taatccaata atagcacatt
     5341 atgatagcgc aatcaacagg gaatttaatt ttttatcttt tcgtttgacc gtatagggta
     5401 atataacagt tagatcacat caactagggt atatctgcgt atacttactg ctaaaataag
     5461 tatatctaat attaactata ccagtaaaac cattcctaca taaccagcgg gaaattccca
     5521 aaatcgtgaa agcatcaaaa atgcatataa cggatcacac aacttcaaca attctgctct
     5581 tgggaaaaat tgatgaattt ggtgagtaaa ggatggaata aacagcattc caacaatata
     5641 ggcaacaata gtgatacttg tttttttggt aaaaatcagt aagcaatagc caatttcaat
     5701 cattccgaga tataaaggta tcgatagaat gatttgggct agtattctta aaaattaact
     5761 agcaaatgtt taaactgatt tatttctaat ctacatgtag taagtatttc aattccacca
     5821 attatcaata aaaataccaa acatatccat
//
LOCUS       DAPCMU010000147         5841 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPCMU010000147 DAPCMU010000000
VERSION     DAPCMU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.152"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            42..1472
                     /locus_tag="RD744_002394"
     CDS             42..1472
                     /locus_tag="RD744_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HDU0558368.1"
                     /translation="MCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRR
                     FQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRL
                     AYVRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDS
                     TTHEIILSFLGKVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPN
                     PFWASIGLSVEPLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDC
                     KICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAY
                     NDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVT
                     TGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1582..1632
                     /locus_tag="RD744_002395"
                     /pseudo
     CDS             1582..1632
                     /locus_tag="RD744_002395"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            1666..3042
                     /gene="tet(L)"
                     /locus_tag="RD744_002396"
     CDS             1666..3042
                     /gene="tet(L)"
                     /locus_tag="RD744_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDU0558369.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKSPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGALIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPTITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIMVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            3606..4868
                     /locus_tag="RD744_002397"
     CDS             3606..4868
                     /locus_tag="RD744_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDU0558370.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5113..>5841
                     /locus_tag="RD744_002398"
     CDS             5113..>5841
                     /locus_tag="RD744_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDU0558371.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPV"
BASE COUNT         2017 a          838 c         1201 g         1785 t
ORIGIN      
        1 tggaaattat aatcaaacag aaagtagagc tgcaccctaa tatgtgtgtg atgagctgta
       61 cggaacctga gcaatgggat gtggtaatag aaggaaatga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattagaac tcgaacaaga ggaaatcaga agatttcaga
      181 attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc
      241 ttatagaagt gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt
      301 gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc
      361 tttatagtgg agtactacat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa
      421 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt
      481 ccggggaaat tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata
      541 caaagctatt gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg
      601 ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg
      661 acagtgaccc gcttctacaa tattatgtgg attctacgac acatgaaatc atactttctt
      721 tcttagggaa agtacaaatg gaagtgactt gtgctctatt gcaagaaaag tatcatgtgg
      781 aggtaaaaat aaaaaagcct acagtcattt atatggaaag accgttaaaa aaagcagagt
      841 ataccattca catcgaagtg ccaccgaatc ccttctgggc ttccattggt ctttctgtag
      901 aaccgcttcc attagggagc ggagtacagt atgagagctc ggtttctctt ggatacttaa
      961 atcaatcgtt tcaaaatgca gttatggaag ggatacgata tggctgtgaa caaggattgt
     1021 atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg
     1081 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
     1141 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
     1201 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     1261 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     1321 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     1381 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     1441 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     1501 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     1561 gaattaatta tgaagtgaaa tgaatgtaac agggttcaat taaaagaggg aagcgtatca
     1621 ttaaccctat aaactacgtc tgccctcatt attggagggt gaaatgtgaa tacatcctat
     1681 tcacaatcga atttacgaca caaccaaatt ttaatttggc tttgcatttt atcttttttt
     1741 agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa tgattttaat
     1801 aaatcacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt ttccattgga
     1861 acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact cctatttgga
     1921 attataataa attgtttcgg ggcgttaatt gggtttgttg gccattcttt cttttcctta
     1981 cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc actcgtaatg
     2041 gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg tcttattgga
     2101 tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat agcccattat
     2161 attcattggt cctatcttct actcattcct acgataacaa ttatcactgt tccgtttctt
     2221 atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa aggaattata
     2281 ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag catttctttt
     2341 cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa agtaacagat
     2401 ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt tctttgtggg
     2461 ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat gatgaaagat
     2521 gtttaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg aacaatgagt
     2581 gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc tttatacgtg
     2641 ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt tcttttagaa
     2701 acaacatcat ggttcatgac aattataatg gtatttgttt taggtgggct ttcgttcacc
     2761 aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc tggtgctgga
     2821 atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc aattgtaggt
     2881 ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt tgatcagtca
     2941 acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat tagttggctg
     3001 gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa gggatcaact
     3061 ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc tttttgttcc
     3121 attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt
     3181 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct
     3241 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa
     3301 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa
     3361 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc
     3421 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg
     3481 acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata ctttacttgg
     3541 aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt
     3601 tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca
     3661 tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc
     3721 gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca
     3781 aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat gctgttcttg
     3841 taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat ccaggagaac
     3901 aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc aagcaaaata
     3961 ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta ggtgttgtgc
     4021 ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc
     4081 tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag cgtggtgaac
     4141 gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact ttggaaaaag
     4201 agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact gcttatagcg
     4261 ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc
     4321 caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac
     4381 caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact gctgcgagag
     4441 ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg gcgagagaat
     4501 ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt cttgtagagc
     4561 gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag tctttaaagt
     4621 ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact aaggaattcc
     4681 ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca
     4741 aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct aaaaagaacg
     4801 aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat cagggaatga
     4861 gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt
     4921 ctttcttatc ttgatacata tagaaataac gtcattttta ttttagttgc tgaaaggtgc
     4981 gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca
     5041 gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata gatgggggtt
     5101 tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc aagggtttta
     5161 gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt
     5221 gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt
     5281 gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg
     5341 tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat gaaacatggc
     5401 attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt tcgttggttg
     5461 tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa gagtttgtca
     5521 gatatggctc aaggatttcg ccgaatgatg caatataaaa aaattaataa aaatcttgtt
     5581 ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta taatcagcac
     5641 atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa ctacgtgaat
     5701 caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga tccaaatgta
     5761 aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc ggcaattgac
     5821 gaaactgcaa aatatcctgt a
//
LOCUS       DAPCMU010000148         5809 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPCMU010000148 DAPCMU010000000
VERSION     DAPCMU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.153"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            450..1604
                     /locus_tag="RD744_002399"
     CDS             450..1604
                     /locus_tag="RD744_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDU0558372.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="RD744_002400"
     CDS             1598..2020
                     /locus_tag="RD744_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558373.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="RD744_002401"
     CDS             3143..4075
                     /locus_tag="RD744_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558374.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="RD744_002402"
     CDS             4092..4607
                     /locus_tag="RD744_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDU0558375.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="RD744_002403"
     CDS             4903..5184
                     /locus_tag="RD744_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558376.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="RD744_002404"
     CDS             5186..5602
                     /locus_tag="RD744_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558377.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAPCMU010000149         5666 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPCMU010000149 DAPCMU010000000
VERSION     DAPCMU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.154"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(131..1177)
                     /locus_tag="RD744_002405"
     CDS             complement(131..1177)
                     /locus_tag="RD744_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDU0558378.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(1188..1460)
                     /locus_tag="RD744_002406"
     CDS             complement(1188..1460)
                     /locus_tag="RD744_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDU0558379.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(1495..2403)
                     /locus_tag="RD744_002407"
     CDS             complement(1495..2403)
                     /locus_tag="RD744_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDU0558380.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(2535..3767)
                     /gene="pepT"
                     /locus_tag="RD744_002408"
     CDS             complement(2535..3767)
                     /gene="pepT"
                     /locus_tag="RD744_002408"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDU0558381.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(3782..4903)
                     /locus_tag="RD744_002409"
     CDS             complement(3782..4903)
                     /locus_tag="RD744_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDU0558382.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(4900..5601)
                     /locus_tag="RD744_002410"
     CDS             complement(4900..5601)
                     /locus_tag="RD744_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDU0558383.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         1629 a         1223 c          977 g         1837 t
ORIGIN      
        1 cgacttatat aaatcttaaa aaaacaaaat tgattgtatg tatataaaaa aagagccaaa
       61 caaacgtaaa attatcttat ccataacgtt tttctttgat ttgataaaaa gggtagatct
      121 tataaaagac ctacccttga ttttctttat tctctttttt tacttctatt atttcttctt
      181 tgatcactct tcgccgttca gcaatcacat acacaaatcc atagagaaat ggcaagataa
      241 aggaactgaa acgatacagt aaaagcgaag aaactgcgtc aactgttcca acaactggtc
      301 tgaataataa caaatagaca aattcaaagg aaccaatacc agcaggcgta ggaataaccc
      361 ctgaaagcac tactgcaaag ctgataaacg aaatggtcag tagaaaatct attttaggat
      421 ggttttcaat cagaatcaaa taaggaagta cataccaaaa tacaagtttt aacatattcc
      481 atagataaat ccgcaatagc gcagtcctgt cttggataat ggtttggacc gtttccctta
      541 aagagtagat ctgctgattg catgtatcca cccatttccg caaacgctca cttttaaata
      601 acttattcga taagctgaca aaaaatattt gaacattcaa gctgacggac atcgccaata
      661 aaaaggcaac aatcacgatc gtcaaaagga taccagcgat gataaaccag atcattgatg
      721 gaccattaga ataaaaaaga gagaattgga tgaccaagcc agctaatgca taagtgatca
      781 ctgccagttt gtacataatc atatggagtg ctgtcacccc tgctccctgg gatagcttca
      841 ttcctttttt gttataaaaa taaatctccg agatcagcgt tcctgtacca aacgaaacga
      901 ttcggtaaaa cgcaatatag ctagaggtca agaacccatc tttcgttgta aaattcggcg
      961 cgaaaggttc agcaatttct ttgattgagc gcccttcagc gatttggtaa actgtcccta
     1021 atataacagc cgctgccaaa acaacaacac tggtagacag caattcggca aagatatctg
     1081 caaatgattg attgattaaa taataaataa tggcaaaaat cacaatcagc aaaagcagat
     1141 tcagaatgat tttagttttc gttcctttag acgacatctc ctgctcctta atggaaaaaa
     1201 taaatgagaa tcaaatcaac aagtacacct gcagcgaatc ctatagtaaa cttctttttc
     1261 cgagttttat gatggaaaac ttttcgagag atcaatcctc ccaaaccacc gcctaacagg
     1321 ctgacaaata tcaaattatt ctcaggaaca cgccagccgc tagttctagc ctgatgttta
     1381 tcatacccca tcagacaaaa taagtaaaaa ttcacaatga aaaggtaaaa ccacaaaaga
     1441 ttgtgtaaca atacatccat tactcttctt ccaactttct gttaactact atttctaagc
     1501 tttttcttct ttctttggtg aataaactgt taatcctttt gatacaacag ataattcaac
     1561 tggaagatca tctgtcgtgt ctccatccgt tcgagtgccc acagattttt cctgcgcttg
     1621 gattttcatg cgtttggctg tgaaataagt aatgccgggg atcgtataga atttgcctct
     1681 catgattcta ggcaaataaa aaataaatcg gaaaatatct aaggaaggta cgatcgttac
     1741 atgaaatttt ccatcatcta gggtcgctga gtcgtcaaaa tttgtaaaac cgcccacgga
     1801 gttagacatc gtgactgtca acagttggga ttttcctttc caatgctcct cgtctgtttc
     1861 taaatccaaa tgatattttt tcttcttcag caataactta aaaaacttct tgataaagat
     1921 ccacgagccg taacgctgtt tttcttcttg actgatccaa actgctgtat ctgctaagat
     1981 tccgattgtc agtgtagaga taatgacttc atcattgact tgaccatagt ctattttccg
     2041 tacattgcct tccaggatca cgtcagcagc ttcttctttt ttgaggggaa tctcaagaac
     2101 acgtgcaagg ttgttgacag ttccacctgg aagtaatccg acatggtatt gagataatgt
     2161 tttttggaac gcttggacga tatgatgaat cgtgccgtct ccacctataa ttactaaagt
     2221 atcagcttct atggcttctg ctttcttgac taattccgca tcatcaatcg atgggccagt
     2281 ttcagctagt tctacatata attctggctg cttttctttt acataatctt gaaaccagcg
     2341 tgctaatttc tcaccctcat ctttccctga acttttgtta taaaaaacta gcagcttttc
     2401 cattctttac ctcctattta ctcttcaaaa aagagggcgg gacaaccaat tccattgtcc
     2461 tgccctttag ccttagacag atgacattcc atcattggga cgaacgcctt gtcataagct
     2521 gttctttttc gattttaact ctttgtgttc aattctgcaa tttttacaat cacgtctgtt
     2581 gctttttcca ttacttgtaa agaaacaaat tcgaatcgtc cgtgcatatt ttctcctccg
     2641 gcaaatagat ttggcgtcgg aatacccatg taagagattt ttgaaccatc tgttcctcca
     2701 cgaactggtt cgatcactgg agagatacca agctcgatca ttgcttgttt cgctaattcc
     2761 acgatgctca tatctttttc aatgatttct ttcatgttgt aatattgatc atacatatct
     2821 actttgattc gttcttgatc aaatcgctga ttcaactctt cttggatatg tgtgatttgt
     2881 gctttgcgtg cttcgaattt ttcacgatca tgatcgcgaa tgatataact catagaagct
     2941 tcttcaggat tcccatccaa tgccatcaaa tggaagaaac cttcgtatcc ttccgttttt
     3001 tctggaactt catcggctgg aagctgatta tgaaaatcga ttgctaactg tagagcattg
     3061 atcattgtat cttttgccgt ccctggatgt acatttttcc cttggatcgt aatatttgct
     3121 tgagcagcac taaacgtttc atactgcaac tcacctaaag gaccaccatc gactgtataa
     3181 gcaaaatcta catcgaattg ctcaacatcg aatttatccg ctccgacacc gatttcttca
     3241 tcaggaccaa aagccacttt gatggtaccg tgagggatag aaggattatt gatcaagatc
     3301 tccattgcag tcatgatttc cgcaatccct gatttgtcat ctgctcccag taatgtactg
     3361 ccatctgttg tgatcaatgt gtggccttca taatttttca aatttggaaa atcttttgta
     3421 ttcaatgtat agcgttcttc cgcatctagt acgatcgtag actcgccatc atagttttca
     3481 atgatttgtg gattgactcc tactgcatta aaatctgctg tatccatgtg ggaaataaat
     3541 ccaatggaac gcacttcatg atcaacattg cttggtaatg ttcccataac gaatccgtta
     3601 tcttcattat agaaaacatc agctaagcct aactcttcta attctttttt caattcttgc
     3661 gcaaaagcaa cttgtgtctg agtcgatgga gtcgttttgc ttgttgcatc ggagcgtgtt
     3721 tctgttttga catatcgtaa aaaacgtggc aaaagatttt cgtacatagt attctctcct
     3781 tttaattaaa ttgaaatggg tttgtatttg tttcagaaac gaaaaagtta atatcccaat
     3841 tttcttcttg tttccaagat tcaaatttct cgataaatcg ttgcttgcat aacgattcaa
     3901 tataatgacc gggatcaatt gcaatcaacc cagcgctttg catatcatga gccgtatgat
     3961 aatagatatc tcccgtgata tagacatctg ctctttgggc gattgcttgc ggataaaatt
     4021 tttcaccgct tccaccgcaa atcgcaatgc gcttcacaga ggattttgca ttttttggct
     4081 ggactattct cagtccatct agttggaacg cttcttttac ttgttctaca aatgcctcta
     4141 tagaaatttc ttgcggcaat tcacctactc gtcctaaccc aaacatttcg actggttcat
     4201 ctatagcgaa aagatcataa gcaggttcct cataaggatg agctgaccgc atggcttgaa
     4261 tcacttgttt ttcgatagtt tccggcaaaa taacctcaac ttttgcctct tgaacttgtt
     4321 ctgtcttccc tactttccca atcgctggtt gtgcaccagc ttccggagta aatcgaccgt
     4381 gtccaatcga ggtaaaactt gtaccggtat aatcaccttg tgtaccagca ccagctgcag
     4441 ctaatacttc acgcattttc tgtgcatgat ccacaggtac ataaacggcc agttttttat
     4501 aatgaatctc atgagtcttt actaaataac tctccacttc aataccgaga agttcacaaa
     4561 accagtcatt cagtccatcc caaataatat ccatgttcgt gtgtgctgca tagacggcaa
     4621 tatcatgttt caacaaatct gcatacattt tttcttgtgg ctgatcagta accagccgtt
     4681 ttacaggacg aaagattgga ggatgtttag caatcagcaa atcgattttt ttcttgattg
     4741 cttcttcgac cacttctgga cgaacgtcta atgtcatcat tactcgttgg attggcttat
     4801 ccaatgttcc gatatgcagt ccgactgggt ctccttcttc agctagccac tgaggacaat
     4861 agctttcaaa cttttggatg aaagtgcgtc cgctgattgt catggcttta aaacctcctc
     4921 aatcatttgg atttcctgct cgatttgttt ttgacgtgcc tcttgattag ctgcttgttt
     4981 gagctgttca cggatagctt ttctttgttg aagctctctt gtccattttg atttgaagat
     5041 ctcacttttc tcttcaagca gaaatggccc gaacatcagc tccatttcat tgtaagtaac
     5101 tggtttttgt tctttttcgg ctacaatgat ttcatatatt tttttatttt cttcaagtat
     5161 tttttctgat tggattttat actgattttc ttgcagccaa gatcgtaatg tacgctctcc
     5221 gatgtttggc tgaaggatca aacgttcttg tccgcttaag cgttgattca acttgccagc
     5281 ttctaggata tctcggatca gtgaacctcc catacccgca atcgtgacag ctgtaatacc
     5341 atcttgtggc tggatagcat ctagaccatt tgcaaggcgt acggtgatac ggtcttctaa
     5401 tccatctttt ttgacttgct tacgtgctga ttcataagga ccttgaacga cttcaccagc
     5461 aactgcatat tcgatttttc ctcttaatat caatgcaacg ggtaagtaag cgtgatctga
     5521 tccaatgtct gctaaacgag cggattcagg gacaaagtca cccactgcgg ctaagcgctg
     5581 agataaatct aaatggttca tagcttcact ccttctagat aattatagca ttttccaaat
     5641 aaaataagga gactaggaat aaaaaa
//
LOCUS       DAPCMU010000150         5603 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPCMU010000150 DAPCMU010000000
VERSION     DAPCMU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.155"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            99..827
                     /locus_tag="RD744_002411"
     CDS             99..827
                     /locus_tag="RD744_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDU0558384.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            943..1188
                     /locus_tag="RD744_002412"
     CDS             943..1188
                     /locus_tag="RD744_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDU0558385.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            1185..1403
                     /locus_tag="RD744_002413"
     CDS             1185..1403
                     /locus_tag="RD744_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDU0558386.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            1539..2078
                     /locus_tag="RD744_002414"
     CDS             1539..2078
                     /locus_tag="RD744_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDU0558387.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            2071..4344
                     /locus_tag="RD744_002415"
     CDS             2071..4344
                     /locus_tag="RD744_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDU0558388.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            4382..5428
                     /gene="holA"
                     /locus_tag="RD744_002416"
     CDS             4382..5428
                     /gene="holA"
                     /locus_tag="RD744_002416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDU0558389.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5498..>5603)
                     /locus_tag="RD744_002417"
     CDS             complement(5498..>5603)
                     /locus_tag="RD744_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558390.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1838 a          904 c         1154 g         1707 t
ORIGIN      
        1 aaaatcttca cgataaaaat aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg
       61 gacgattttg ccagagctca ctgtttcttc aattttaaat gaaaggagta atgaatgatt
      121 tttactggtc attctttgaa aaaagaacaa tgatagaaaa aatcagcaag aaaatactag
      181 cttcacccaa attgatcatt tggatgtcta tgatagttct tatcttttgt atcgcaagtg
      241 gttttttatt atcaaatttg ctattttata gagaaacaga agagactgct gcaaaagaaa
      301 tactttcttc gtttagttcc agtggagaag atccatcagc tgaaggaaac gatgtagaag
      361 aggatgctcg tccaaaggtc atgtacaccg atatcaaagg aagtgtgaag gagcctggta
      421 tctattcctt ttcttcagaa gaacgggtct atgatgtact aaagcgtgca ggtggattat
      481 tagaagaagc ggatagcgat cgaatcaact tttctgcgaa gatagaagat cagcaagtcc
      541 tatatatccc tgctgtaggt gaagagctgc cagaacatct tgatcagttg gcatcctcgg
      601 aaggaaaaca gcctacagca gattccgagc ctcccaaaat aaatatcaat acagccactc
      661 catcagaact tcagcagatt cctgggatcg gatcagtcaa agcgcaagaa atcattcgat
      721 ttagagaaga aaacggatcc ttccagaaag tggaagacct gcaggagatt tctggtatcg
      781 gtgaaaagac agttgaaaaa ctaaaaaact tcgttacaat taaatagaat gattcaaaat
      841 cgactggcac gagcaataaa tggacgctaa tggatactaa gaataatcca attctaagag
      901 cttggcagga cgaggaggta gtatttttgg ataggaaata gcttgattgt tcgcaaagaa
      961 acattaaaaa aaccaatgct gaatgtctat ctccaaaata aaatatctgg gattcatatt
     1021 atgaatacgg ctgtcagcgg caataacagt caagctctac gagaacgctt tgccaaagat
     1081 gtacttagct atacagcaga taaagtattt attctcattg ggacgaatga tctagcagag
     1141 cataaacaac tttctaaaga aacgtaccaa aaaatctgca gtggatgata gttcagctga
     1201 aaaaagacta tagttctgat catgctttct ttatcacgcc accagcagtc gatgagacaa
     1261 aacaaaaata tcggaataac aagttgatca aagaatatat ctctgttctc acagaagtag
     1321 caaaacgcca tgattgtgaa gtatcgattt ttatacggaa tttcttgaac aaatggattc
     1381 gaagaatttt tataatgggc taatggatga cggacttcat ttcggcagtg aagggtagga
     1441 tcttttgtca gacaggatga ttcgttatat ttaaagaata ttttgtttaa gcaatgagta
     1501 ctttttaatc aacttgaaaa aacggaggaa tataaaacat gttagaaaaa tcttcaaaag
     1561 catgcgatac gaataaagaa aaagttactg ataagtcaca taatgaacgc attccttggg
     1621 accagtactt tatggctcag gcagtactgt tatctttacg tagtacgtgt acacgtttag
     1681 aagtaggagc gacactcgtt cgagaaaaaa ggatcattgc tggcggctat aatggagcag
     1741 tcagtggaga tgtccactgc atagatgaag ggtgttacgt cgtggatgga cactgcttgc
     1801 gaacgatcca tgcagagatg aatgcactcc tacagtgtgc aaaacttggt atcccgacgg
     1861 aaggttcaga aatctatgtc acacattttc catgtttggc ctgtacaaag gctttgttgc
     1921 aagctggtgt gaaagggatc aattatctcc atgactatcg aaatgatcct tatgcccaag
     1981 cgttgatcga acaattaggt gtatctgtac accaagttgc tttagactca cagcattttt
     2041 caaaacttca aaatgaatta acaggaaaac atgtctagcg ttttagaaag gcatgttcga
     2101 aatcggttga tttttccggc aattacgtcg atgattatct gtttgttgta ttacactgac
     2161 aattggggtt atcgtttgct tcttgccgga atttttttat ggttttcgct aaaagaagga
     2221 cgttttatcg cgtacttgtg tattttgtgt agcataacgg tatccatttc ttataggcta
     2281 caagagtatt catttttaga ggaaaaaaca acatctgcgc aaattactgt atttacagat
     2341 acgattcgtg caaatggtaa ttttttgaca atggaaggaa ggttatcaga gaataaacaa
     2401 aagctccagc tttcttatca agtaaacaca gaagcagaat tgaaaacatg gcttaacttc
     2461 cgaggaaagg gaatagaatt gactgctgaa ggcgagttca tctctccagc aaaaaagcgg
     2521 aatctttata attttgacca agaagcttac ttcaggaacc atagaatttc tggacggttc
     2581 caaattcaaa agattgaaca gatcaccatg caaaatcact ggcgaaactg gcttcagaga
     2641 aaaagaggat cttttatctc ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat
     2701 attaattctt tattatgggg ctataaagat gcaggattca gagaaccaga agaaattttt
     2761 agggagagtg gtttgctgca tctgttcagt ctatcgggtc tacacatcca attttattta
     2821 gggtggctgt attatttatt tcgacgtatc ggatggccac tacacgtcag tattattccg
     2881 ttgagtctac taactatctg ttatgtttct attgcaggaa gttctatttc tgttttgcgt
     2941 gctagtttgc tgtttttatt gcgattgcta gtcaaattgc tgaataagca ttattccacc
     3001 atggacttat ttgcagttgt cttttggctt cttttactat ttgatcctcg atttcttttt
     3061 catgcgggtg gacagcttag ttttttcatg agttttttgt ttatcctgat ttcttttgat
     3121 ggagattggt tgaaaaaaac ggcctctcac gtcctgttta ctatgatcac gatgccgttg
     3181 attgtatggc atttttatga gtggcctatt ttaggtagtc tttttacatt actgattgca
     3241 ccggtattca agtttctttt tcttcctggt gtgttatttc tcacactctt caatcgctgg
     3301 ctgccggaaa ctttactttt gctgatagaa gagggattaa tcttatttga gcgaataatc
     3361 gatttttctt cagggtcgtc tctcattata ggacgtttgc cccttgtgct atctgggatg
     3421 tatattttcg gattactgat ttgtatggat aggttgaaag aacaacctgt gaaacacttg
     3481 ttttatgttg gtcttttttc ttttattttt ttgactttgc catttttacg cttttcttca
     3541 acgatcgctt ttatcgatgt cggtcaagga gacagtatct ttttgcaaag tccttttcga
     3601 aaagaaacga ttttgatcga tactggaggt cagttacact ttgaaagaga aaagtgggcg
     3661 caaggtatga tacgtcctcc tgctgaaaga aatatcgttc catatctaaa aggtcagggg
     3721 ataagtgtaa tcgataaact catcttgaca cacgacgata cggatcatgt gggagaatta
     3781 cccacgttat ctcagcattt tactatcaaa aaaatttata tcggttgggg cgctggacga
     3841 aatgagccat tacaaaggca tttatctgaa gctcaaaaaa atggtactga gatcattgaa
     3901 atcaaacatg gggacaggat cagcggttat tatgatcttt atgtattgac tccttttgaa
     3961 aagggagagg gaagaaatga agattcgatt ggtctatgga tggaatataa caataggcgc
     4021 tttttatttt taggagattt gaatcaagca atggaaaaac aattgctttt gatttatcct
     4081 aatctgaaag ccgatgtagt caaattaggt catcacggta gccgtacgtc aagtaacaca
     4141 gattttcttt cccatattga agcaaaacat gggattatat cctgcggcgt aaataatcgt
     4201 tatggtcacc cgcacagcga agtattggag acactagaag aaaatcagat aaaaacatgg
     4261 cgaacggatc aacaaggaat gatcacctat cgctggcatc cagtttttca tcctcatggg
     4321 ctagtggaaa caatgataga ctagtaattc tcatctgctc atggtaggat aatttttgaa
     4381 agtggtgagt aatttgagta tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc
     4441 atcttgttac ttgattttag gaacagaaaa gtttctccaa gatcaagttc ggacagaaat
     4501 tgtcaaaaaa atccaattag agggcgaaga tgacttaaat tttttaagtt ttgacatgga
     4561 aaatgcaagt ttagatgagg tagtagctga agcagagacg ttgccgtttt tcggagatca
     4621 acggttagta ttcgtagaga atccttattt tttaacaggt gaaaaagtaa acaatggaat
     4681 cgaacaaaat actgatttat tgacggctta tttgaaagaa ccattagaat cgactgtatt
     4741 agtattcttt gcgccatatg aaaagttgga tgaacggaaa aaagtaacga aacaattgaa
     4801 aaaaacagca attatcatca atgtccaaca gttgaatgaa aaagaagtac gtcaatatct
     4861 gatgaatacc ttagagaatt cctcaatcaa aatggaacga agtgcaattg atttattctt
     4921 gaggttgacc gacttagatt tatccaaaat gatgagagag ttacagaaat tggtccttta
     4981 tggacaaaat caacagcaaa tcacggtaag agaagtagaa gaattagttc ctaaaacgtt
     5041 agaacataat atttttgaca tgacccaata tattttaaaa gggaagacgg aacaagcttt
     5101 gcgtttatac gaagatttag ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt
     5161 gctttctcaa ttacgtttat ttttacagac aaaatttctt gtaaagattg gttatcagca
     5221 ggcaaatatt gctgaaacat tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga
     5281 agtaaaaaaa ttcgatgaac atttgctggt acagttgttt gatcaattag ttgagatgga
     5341 ttatcaaatc aaaacgggac aaatcgaaaa agaattaagt tttcaactat tcgttttgcg
     5401 cacagggcag ttgttaagaa atcgataaga agaaagacga gggcaaagca tgcgggaagg
     5461 tcgtttgttt ttgtcctttt tttgacaatc ttaattgtca aattaagtta gacagatcat
     5521 caatacttac gtaactcaaa aatacttcca aaggtgttcg ataatccagt gattttctag
     5581 gaatattatt tcgtttagat gcg
//
LOCUS       DAPCMU010000151         5367 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPCMU010000151 DAPCMU010000000
VERSION     DAPCMU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.156"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(1..129)
                     /locus_tag="RD744_002418"
                     /pseudo
     CDS             complement(1..129)
                     /locus_tag="RD744_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390190.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
     gene            complement(482..1171)
                     /locus_tag="RD744_002419"
     CDS             complement(482..1171)
                     /locus_tag="RD744_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDU0558391.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1216..1980)
                     /locus_tag="RD744_002420"
     CDS             complement(1216..1980)
                     /locus_tag="RD744_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDU0558392.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2008..2706)
                     /locus_tag="RD744_002421"
     CDS             complement(2008..2706)
                     /locus_tag="RD744_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDU0558393.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2719..3498)
                     /locus_tag="RD744_002422"
     CDS             complement(2719..3498)
                     /locus_tag="RD744_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDU0558394.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3514..4458)
                     /locus_tag="RD744_002423"
     CDS             complement(3514..4458)
                     /locus_tag="RD744_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDU0558395.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4499..5278)
                     /locus_tag="RD744_002424"
     CDS             complement(4499..5278)
                     /locus_tag="RD744_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558396.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         1390 a         1128 c          946 g         1903 t
ORIGIN      
        1 tttcagaata attacaatat tttgggaccg gataactccc ttttgctcgt aaccactcat
       61 atccgttagg attcactaaa agtttaccgt ttatttttcg attctttttt tgaaccgtcc
      121 catgaacata ccgattcaac aagctaaatt ataaaaattg gtgtgctgtc attatttttt
      181 ggaaaattta ttaatggcta tcaatatact tttgtatcat actaaatagc tcgtcagttc
      241 ctatatcgga tataccaata ttaaagcatt tagatcatta tatttaagag tggtatcatc
      301 ccaaaatttc gaatattttc ttttatataa gaataatgat actgaattgc cccattcacc
      361 tttctttttt tagattttct acaagtgatt caaaccctcc ataattacag ttattacttt
      421 tgaaacgata atatgaacat gtgtaatctt ttttattaac aatccatcat tctatcccta
      481 actaatatgc tccattgggc ttgacaataa cataaaacgt ttttaaaatc aacctcaagt
      541 cgtacattaa acttcgatga ctaatatact ctaaatccaa ctcaaccatt ttatggaaac
      601 taacattatt tctatcacta acttgccaca aacctgaaca accaggtttt acatacaagc
      661 gttgaagatc atatctcgtg tactcagcta cttccctctc caaaggtggc cgtggaccaa
      721 ttagactcat atcgcctttt aaaacattcc atagctgtgg caattcatca atgcttttgg
      781 cacggataat tttacctatt ttagtgacac ggggatcttc tttcatcttg aacattgcac
      841 cttctatttc attttgatca agtaattctt ccaatttttc ttcagcatct aaacacatcg
      901 aacggaattt atacattttg aatgtttttc catttttacc aacacgtgta tggcaaaaaa
      961 atatcggact tcctggctct tcttttctga ttcgataagc tacatagaac atcaaaggag
     1021 aagctaaaat caaaccaata atgcttccta caatatcaat cattctttta aaaaataaat
     1081 agatatatcg tttttcaact ttttctttat ccaaccaaac ttgattagga tgtacttcac
     1141 taatacgaga taataaatct tcactttcca tactttccct ctctcctcat ttccttttta
     1201 tctacgaata aagaactaaa ataaaccaaa cttcttcttt tttacctctt catagactgg
     1261 ataactgatt tcatccccgt tgatcagatc ctttgcgact tgtttcatct gtactacttt
     1321 ttccttacca aagtccttag caatctgctc atagcactct tttaagtaaa aggtacgctt
     1381 agagacccca tgggcatcag aagcagccat ctgcacaaga ttatgtttga ccatcatttt
     1441 ggctgttttc tggatgtctt ttccaaatac accgacataa cttggtgcgg tgagttgggc
     1501 caagcatccc atatctagga atggcagcat atgattcggg tctttacgaa aatgcgcgtt
     1561 gcgttctgga tggacgatca ctggtgtctt tcctagttgt aacaactcaa aaaagagctg
     1621 ttctgcatat aacggcactt ccagtgtcgg aaattcgatt agaagatatg tatcatctaa
     1681 atctgtaaat aaaatttcat ctcggtcgat gtcttcgatc agtgttccgg taatacgtac
     1741 ttcttgtcct tccaataacg tcaaaggcaa ttgtcttttc tctaattctg cttgtaacga
     1801 agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat tatgatgggg
     1861 tgtgcataaa atatgtgtga tcccttgttg aatcgctttt tcagccatgg cgatactggc
     1921 atctatgttt tctgctccgt catcgattcc tggcaggata tggcaatgta gatcgatcat
     1981 ttctcttgtc tcctctcttt ttatttctta attcccgtag taatagccag catctttgct
     2041 gtgttctgcc ccattgtata caacaccaag gactcgcgct tgtaccatat ccagcaattc
     2101 ttttgcttta gtcaatgatt ctttccgtga catattttca cgtacgacca ataaggtgcc
     2161 atccgttttg gaagacatga tctgtgcatc tgttacggca actactggcg gcatgtcgaa
     2221 gatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc ttggcgaact
     2281 tagtagttct gatggatttg gtggtttagg tccacttggc agaatcgaca aattctcgac
     2341 tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagaacgg tacttaatcc
     2401 gcttgcgttg ttcaacttga acgttttata gatgacgggc ttacgtaagt ctgcatcgac
     2461 tagcagcacc cgttgaccag atttggcaaa aacaacggca atgtttgctg cagtggtcga
     2521 tttcccttct ccaggtcctg atgaagtcac aacaatcgtt ttaatttgct gaccagctgc
     2581 ggaggcaaac tggatattcg tccgaatcgt acgatattgt tcagcgattg gtgaagaggg
     2641 gtttgtgagt gtgaccaaac tgactgcatg agtttgggct gctttttgtt tctgtgttcg
     2701 tgccattttt ctttcctcct agactcttga gcggcttctg cgtgatggcg ctgttttatc
     2761 gtttgtagct tttggttgtg tcggagctgg tcctttttta cggataccag cattcaattc
     2821 tttcgctgac atttctggta cagtccctaa gatcgggaag cccaatgtat ccgtcacata
     2881 tttttcatct tttactgtcc ggtcgagtaa ttcaaataag aaagctagac ctacgcctac
     2941 catcattcct aacactaagc caatcactag attcaattta ttatttggcg aaacaggtgt
     3001 cgtattggca actgcacctg agatgatcga gattttatcg acattcatca cgtctttggc
     3061 gttctcttgg aatacttgtg acgttgtatt agcgatttgt tgagccgtca ctgcatttgt
     3121 gcttgttgct tggatcgaga acatttgtga attttgtgac tgattgacgg agatggcatc
     3181 tttgatttgt ccagcagtca tttttacatt gtcttctgtt tctaaacgat ctttgacttc
     3241 atttagtact agatcactga cgatcatatc cttatacgta ttgatcattt gcaggttcgt
     3301 attgacatca ttgacatttg cagtatctga ttgcggcagt gtcacgatca attgggcttg
     3361 tgagctgtat tttggcgtca agatgaaaaa cgtcacgatg cctgagatgg ccaagccagc
     3421 aaacattgag atgaggattg ttatcacgtg tttgcgtaat gtttcaaaaa tttctttcaa
     3481 gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc
     3541 ttttaattct tttttcacac gatcaagttc ttgatcgctg actttttggt atgagatatt
     3601 gtcttgcatg aagccttctc cttgcagctg atcttgcttg atcgtaccaa atgcatcccg
     3661 ataatctaaa gcaatctttt tcatttcatc aaagctcatg tctgttttca tattcgactc
     3721 tagagcattc aaaacggatt gatagttgct tacaccgttc agactcaaca cttttttcgc
     3781 tacaccttct acgatctttc tttgacgttc ttgtctgccg tagtcaccat ttggatcttc
     3841 ataacgcata cgagaataag acaatgcctg atccccgttc aatgtgattt taccgatcgt
     3901 aaaatcatag ccatcttgtg agaaggtgag gtcattattg acttcgatac caccaactgc
     3961 atcaacgagt tcttttagac ctttcatatt gattgaaaca tagtgatcga ttgggatgtc
     4021 tacatatttt tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt
     4081 gatcttgtct tttgtgtcat gtccaacgat gtcgacatac gtatcacgag gaatgctgac
     4141 aatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc
     4201 ttgatcgacc cgacctaaat ctcccgtatc aatccccatc aatagaacag agaaagattc
     4261 ttgtttgtcc agatcaactg gttttgttcg ttctccttgg gttcgttcca ctgattcgta
     4321 agttttttca atagaatgtg agagatctag atataatttt gctcctactc ctacgattgc
     4381 taatactaat acgacaagaa ttgctaaaat aattagaaat atctttttcc ctcttgatct
     4441 tcttcttcgt tttgccatag tttcctccgt ttgctagcct tgcttgaatt ctcgcttatt
     4501 aatagctcgt attgctatag cccatttgct gagcgactgt ttctgctttt gacgcgtatg
     4561 cggctcttat tttttcattc cggatctgtt cgatgagact gagtgcggtc aaatattgat
     4621 cgtaagtagc agtagctgtt acttgaccgt ctttcagcat cgtatcgaat agctgatcta
     4681 gttgcgtgac ttgcgcgatc tgagtatcag cgaatcctag atattgcata acaaccgttt
     4741 tccaaggact atctttaaaa aagctcatat tttcacgtac acgagcgaca tctgcacttg
     4801 tcaccttttc tttgatgacg acatcgtcta cagcttgctg ccagtttgat acatttttag
     4861 taaataaatc gttgacagct gtctggatct tcagttttga ttctgcatcc tctaactgcg
     4921 ttaagactgc tttcttttct tcttggatcg cagcagcttc tttaggtaat tctgattcct
     4981 tgatgtcata atcatctgcc gaaagtttga tagaagatag tttatgtgac aaatttgaaa
     5041 ctgtttcttc tgttatttca ggatttagga aaattttttc ttttgtactg tacagcttat
     5101 ctacttcttt tgataaagag gtcacggtgt ctttttgatt ggtgatggca gattctgcgt
     5161 aattgatccg ttgatgatgt tgccgctgat tattgaaatg gtatccaaac aagcaaatca
     5221 caatagcgcc tgtgcctacc gccacagtag ccaataactt gacgaacggc ttttccatag
     5281 attcactcct aaatattgat gatagataaa taaagatgtg attcattccc atcagaatga
     5341 gcgcttttta ttttaaaagc ataatta
//
LOCUS       DAPCMU010000152         5116 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPCMU010000152 DAPCMU010000000
VERSION     DAPCMU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.157"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(117..1265)
                     /gene="nagA"
                     /locus_tag="RD744_002425"
     CDS             complement(117..1265)
                     /gene="nagA"
                     /locus_tag="RD744_002425"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDU0558397.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1341..2177)
                     /locus_tag="RD744_002426"
     CDS             complement(1341..2177)
                     /locus_tag="RD744_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558398.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2361..3578)
                     /locus_tag="RD744_002427"
     CDS             complement(2361..3578)
                     /locus_tag="RD744_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDU0558399.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3727..3906)
                     /locus_tag="RD744_002428"
     CDS             complement(3727..3906)
                     /locus_tag="RD744_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558400.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3961..4044
                     /locus_tag="RD744_002429"
     tRNA            3961..4044
                     /locus_tag="RD744_002429"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3995..3997,aa:Leu,seq:gag)
     gene            complement(4284..>5116)
                     /locus_tag="RD744_002430"
     CDS             complement(4284..>5116)
                     /locus_tag="RD744_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558401.1"
                     /translation="VNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTP
                     GNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYR
                     KCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTK
                     LSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPT
                     VWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR
                     "
BASE COUNT         1589 a         1044 c          815 g         1668 t
ORIGIN      
        1 aacaatagga aattaaagaa acttttgaaa cttatgtaaa gtgattttta ctagaacaca
       61 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
      121 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
      181 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
      241 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
      301 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
      361 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
      421 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
      481 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
      541 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
      601 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
      661 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
      721 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
      781 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
      841 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
      901 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
      961 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     1021 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     1081 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     1141 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
     1201 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
     1261 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
     1321 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
     1381 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
     1441 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
     1501 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
     1561 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
     1621 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
     1681 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
     1741 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
     1801 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
     1861 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
     1921 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
     1981 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
     2041 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
     2101 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
     2161 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
     2221 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
     2281 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
     2341 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
     2401 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
     2461 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
     2521 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
     2581 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
     2641 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
     2701 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
     2761 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
     2821 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
     2881 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
     2941 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
     3001 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
     3061 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
     3121 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
     3181 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
     3241 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
     3301 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
     3361 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
     3421 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
     3481 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
     3541 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
     3601 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
     3661 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
     3721 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
     3781 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
     3841 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
     3901 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
     3961 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
     4021 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
     4081 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
     4141 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
     4201 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
     4261 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
     4321 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
     4381 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
     4441 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
     4501 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
     4561 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
     4621 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
     4681 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
     4741 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
     4801 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
     4861 atgtctaaaa aaaacggctt tccaaatgat gaaataccta aatctaactt attaagaaag
     4921 ttgaaatttt tatattcagg aaaccattta ttaatttcat gtgcagcacg taatgcattc
     4981 cctggagttg tcaaagagaa aattaattct gctaagctta ttattgaaaa tactgataac
     5041 ttataattat atcgtccatt gacaatcaaa tataaactaa ctatacttgt tagaacaaaa
     5101 aaacaggcgt tgactt
//
LOCUS       DAPCMU010000153         5111 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPCMU010000153 DAPCMU010000000
VERSION     DAPCMU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.158"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..152
                     /locus_tag="RD744_002431"
     CDS             <1..152
                     /locus_tag="RD744_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558402.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="RD744_002432"
     CDS             complement(230..763)
                     /locus_tag="RD744_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDU0558403.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="RD744_002433"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="RD744_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDU0558404.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="RD744_002434"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="RD744_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDU0558405.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="RD744_002435"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="RD744_002435"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDU0558406.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="RD744_002436"
     CDS             complement(4488..5066)
                     /locus_tag="RD744_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDU0558407.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1469 a         1049 c          918 g         1675 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat t
//
LOCUS       DAPCMU010000154         5088 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPCMU010000154 DAPCMU010000000
VERSION     DAPCMU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.159"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(205..660)
                     /locus_tag="RD744_002437"
     CDS             complement(205..660)
                     /locus_tag="RD744_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558408.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="RD744_002438"
     CDS             complement(1269..1685)
                     /locus_tag="RD744_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558409.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="RD744_002439"
     CDS             complement(1687..1968)
                     /locus_tag="RD744_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558410.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="RD744_002440"
     CDS             complement(2415..3383)
                     /locus_tag="RD744_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558411.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4550..4705)
                     /locus_tag="RD744_002441"
     CDS             complement(4550..4705)
                     /locus_tag="RD744_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558412.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4724..>5088)
                     /locus_tag="RD744_002442"
     CDS             complement(4724..>5088)
                     /locus_tag="RD744_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDU0558413.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1595 a          839 c          839 g         1815 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DAPCMU010000155         5077 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPCMU010000155 DAPCMU010000000
VERSION     DAPCMU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.160"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(406..780)
                     /locus_tag="RD744_002443"
     CDS             complement(406..780)
                     /locus_tag="RD744_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0558414.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="RD744_002444"
     CDS             complement(803..2203)
                     /locus_tag="RD744_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDU0558415.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="RD744_002445"
     CDS             complement(2207..2617)
                     /locus_tag="RD744_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDU0558416.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="RD744_002446"
     CDS             complement(2635..3126)
                     /locus_tag="RD744_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDU0558417.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="RD744_002447"
     CDS             complement(3136..3927)
                     /locus_tag="RD744_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDU0558418.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="RD744_002448"
     CDS             complement(3920..4693)
                     /locus_tag="RD744_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0558419.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>5077)
                     /locus_tag="RD744_002449"
     CDS             complement(4849..>5077)
                     /locus_tag="RD744_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0558420.1"
                     /translation="SFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLNVSK
                     DFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1621 a         1030 c          667 g         1759 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaactt
//
LOCUS       DAPCMU010000156         5024 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPCMU010000156 DAPCMU010000000
VERSION     DAPCMU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.161"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            398..604
                     /locus_tag="RD744_002450"
     CDS             398..604
                     /locus_tag="RD744_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDU0558421.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(806..1807)
                     /locus_tag="RD744_002451"
     CDS             complement(806..1807)
                     /locus_tag="RD744_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDU0558422.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1812..3725)
                     /locus_tag="RD744_002452"
     CDS             complement(1812..3725)
                     /locus_tag="RD744_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDU0558423.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3893..4399)
                     /locus_tag="RD744_002453"
     CDS             complement(3893..4399)
                     /locus_tag="RD744_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0558424.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4559..4999
                     /locus_tag="RD744_002454"
     CDS             4559..4999
                     /locus_tag="RD744_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDU0558425.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1389 a         1007 c          768 g         1860 t
ORIGIN      
        1 ttttctaaaa aattaaaaaa aattttggtg aattaagtat ttccttgata acactggctt
       61 ttggcctcat tttaggttat catttgtaat tttatttcac agttgatatg attgttatgt
      121 aaaataaaaa agtttaggat acatcgaatt tttggggaaa ttcttttggg ggtatcctaa
      181 atcttttcta ttatattccc atattttcta ttattggtct agacgtatga tggtaaactt
      241 tgtacctatc tgattgctga atcattaatt gcttatcatt agccatcttt gtttcaagtt
      301 cgctagatga tcattttatt cacatcctaa caaacttgtc acatttatag attcttaatc
      361 tatttatcat acaattataa aaaaggaggt gctgcttatg aaagctatcc atatttcttt
      421 gctttttttc gctagtgcat tgattttatt tgctattcat ctttgttccc agccttatat
      481 caataacaac ggaatattag tggaaccagc attcttttgc ttaccactcg gctttttgtc
      541 ccttcttgcc tccgcaggct tcggatttgt tgcttttttt aaacgatcaa aacaacaaat
      601 ctgacttata aaatttacgt aaaagagatc gtgacaataa tcgtgtgata ttcccaaatt
      661 tcgaataaaa ggtgcccctt gccatctcta actcctcagt cacaagttac aatattcata
      721 aaccatgaaa agcttgttta ttgaaattgc tccttgtgac ttggagctaa gtgacagatt
      781 acaacctctt ttgcatcata agactttact ccttgaaaaa cattactaac ttttgatatg
      841 tttcttgcgc ttgtttattg ctgaaatcaa actgatattc atggttcaaa acagcatttt
      901 tctccgcata atctactttt gtgactttca cccccgcttt ctcaaataca gaagttactt
      961 cttccatttg tttcgtaaac gtatttgtat ttccatccgt taagaatgtg ttttgcgtcc
     1021 aacgatccgc atgtctgacc aaggctaaac ttttgacctt ctctgattgt tcaaagtcct
     1081 tattatcaaa gtaagcccat ccacattttt cataaagaaa gctagagatt ttatcactta
     1141 cttgagtaag tgcagaagga tccaacagac cagacaaaga aataaacttc gtaatctgct
     1201 tgttctccaa agtcggatca agatgatcag cttttttaat ttcttcattt tgaagtgcta
     1261 acaaatattc tccactaatt tgtgcacctg ccgaatttcc accaatcgaa atattcgacc
     1321 aatctgcttg atcttttatc tcttttttag ctgcttgaat ggcttcgttc aattggataa
     1381 ctggtgtagg atgttttgct tccggtgatc gttcataatc tatatttacg atgatttgct
     1441 gcgtgtctga acatagatat tccaaataat cacgacaatc atttttatcc cctcctacat
     1501 atgcgcctcc atgcatccag aaaagaatcg gtaatttttt cttttccact ttcggtgtgt
     1561 agatatccat attattattt ttgtactttg aaggatagga tatttctttt tttatatcga
     1621 tttgttcgat tttttgctgg aaatcatctg gtcgggcata tacttgttct ttttcacttg
     1681 tgtcaaattg ttttctaaca agcaagctaa tcggctttgg tgtgatttga ttaatcaaaa
     1741 atacagtgac taaaataact acgatgataa ctggtactgc aatcactatt cgcttgaacc
     1801 atttcattca tttaccctct ctttcttcgc caaatccatg tgataatcgt gctaacagat
     1861 aaaaatccac ctgaataaat gagaagcgaa tgtttctttt ctccagtttt cggcaacatt
     1921 cttttttttg catctgtctt tttttgtact ttcactgtca tctgaatcgg ttgcgtatca
     1981 tcacttgatt gatcctctcg aggttcattc tttcccttat tgtctgcatt ctctgtcgga
     2041 tcttgctttt ttggtggagc tactgggatt tttttgtaat agaaaataat agttggtatc
     2101 gtctcattaa aaatcccttc agtcggtcct tcaacatttg ctaattcgta tccttcgaat
     2161 gtcttttgct ctgtcatata attttctcca taagctccgt aaagctggat ttcgtcctgg
     2221 tctttgagta gacttttatt gttttcatcc acatacttta cgtttatctg atcatggaac
     2281 actactttca aaaagtccgt tacatgtttc gtgtaggcag catggtcaac ttcctgtgcc
     2341 tttccatgcc ctgctccttc tactaataaa agatctttat accctcctga taactcatat
     2401 aatttttgcg cgttagacac aggtactaca tcatctgctg ttccatggat aatcaattta
     2461 ggcaatgaat ctgacattcc aatcgtaggt agagcagctt ttgtatccaa atgcatctcc
     2521 tgatcaaaat agtattgttt caaaaggccc ataaataaat cttcatgctc ttgatcccca
     2581 atgatgtcta actgattttt tatggcatcc ggtagtgcgg gggctactgt ctggctgtac
     2641 gtaaaacgta gttgttcatc aattgatgca aatccgcaat tttcaataat tcctttgaca
     2701 ttgacaggaa tatctttcgc taatacagac atcatcgtag ctgctcccat cgaaccacca
     2761 taaagtaaaa tcttttgaga tggccgctct cgaacttctt gatttatcca gtaaaggacg
     2821 tcatctgact catattgtcc ataggtcaca taatcgcctc cgctattacc agtcgctctg
     2881 gaatcaacaa ataataaatt atacccggcc ttataaaaat cattgtattc ttctgtaacg
     2941 atcccgttat tccaattccc tcgaaatccg ccatggatca tgactgtttt atctgaacct
     3001 tgatcgacat aatatgcata tacatccatc attttgccat cttgtttgtt tagtacagtt
     3061 tgatgaagtt gttttgtatt tggatagaag cctgtgtttt ctgtaggcgt tgtcccatca
     3121 tctgtacctc caagcatcga catcaacaca ctgttttttg caaaagcgat atcgtacaat
     3181 agataagtag caatcagatg tttgataatc tctttatcta caccataatc attaacagca
     3241 agctcagtga ctaataactc tattaatttt gacttgatgt cttctgttgg tccattaagt
     3301 atttctgtca tctgtttacc gaagtaattc aaatattctt taccattgaa attttcacct
     3361 ttaccgatat catcattttc tgtcccattc tctttgtcac ttactttagc agaagaggat
     3421 tcgtcgtttg acatttttgt agctgttgct tcttctgttt gttggttatc tgtggtagcg
     3481 gtcgtttctt gatctgtgct agtaacagat gtctcaaccg ttgtttcagt atttgtttct
     3541 gtttgtgaag tactcgattt cgtttcattt ccagcatcag tagtttctgt ggtttctacc
     3601 tcccgagatt ccgattcgga agaaatgctc tctccatata ctgtccattg cgggacgctt
     3661 gtacttacag caaataagat gaccaacgct ccctttaata cttgttttgt tctcaagcta
     3721 ttcactcctt tttatgtttc taggagaatt atatgtaaac gcaaacattt tttatacaaa
     3781 aaataaatgg tttatcccat aaaaatagaa caggattatt ttctcatttt acgtaataaa
     3841 gcagaatgga taaaccagta tccattctgc tttttttaaa cgaagaaaca acttattgct
     3901 ccaaaaatgt cagaaatcgt ttatcccaaa ttttctcgta aaaagcgatt gtttcctttg
     3961 ctttcataaa aggattatca taagttgtcg ttgctcctct acacatcata agctggtatc
     4021 ctaatccatg agcaaacttc acagtcgtat ctatacaata ctctgcttgt gccccacata
     4081 attcgatcga atgacagtcc aattcagtta ataaaacact caaattagtc ttataaaatg
     4141 aattcgcatg ggtttttctc acaaaataac tgtgctcagg aatagataat tccgacaata
     4201 gctcccatgg ttttgtttgt ggcactaatt gagcatcttc gtgttgaacg aaaataactg
     4261 gtttcccaat cgtttcgtat gttttgattc ttttattcac taatgctgtc actgtttcta
     4321 attcgtgtat cggcgaagaa tcaaaacata cccctttttg taaatcgatc actactattg
     4381 cgtcggctgt gtttgacata cttgtgcctt ctttcttttt tcttgattta atagtatttc
     4441 ttatctagct agttccatta tacgtgacaa cattttctta ttttacacct ctaaatggca
     4501 cttttcgact atttcttcat gttattattt cataaatact tagctattgg aggaatgtat
     4561 gatgattaaa ttgtatacag gttctggctg ctcgtcttca agacgcgcaa aacgttggct
     4621 tatcgtccat gatataccgt ttatcgaaca gaacttaatt cgtcaaccac taaccaaaaa
     4681 tgaatttatc cacttactaa aattgacaaa caacggcgtg accgaaatgc tctctactca
     4741 ttctattagt tataaaaatc ttgttcacag aatagatgaa ctcagtttga gtgaactata
     4801 tattgagatt cgtacatccc cttctttatt gaaaaaaccg attatctacg accaatctgc
     4861 aaaacttttg actggattta gcgaagataa aatcaggatg ttcctttcta aaactaaaag
     4921 aaagcaagag ctcaacgaac tcttgctttc tggtcatctt gtcgatttta aagaaaataa
     4981 tctttttgca atgtcttaat tttggcataa acaatctgct tttc
//
LOCUS       DAPCMU010000157         4885 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPCMU010000157 DAPCMU010000000
VERSION     DAPCMU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.162"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..258)
                     /locus_tag="RD744_002455"
     CDS             complement(<1..258)
                     /locus_tag="RD744_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558426.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="RD744_002456"
     CDS             complement(274..924)
                     /locus_tag="RD744_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558427.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="RD744_002457"
     CDS             complement(935..2107)
                     /locus_tag="RD744_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDU0558428.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="RD744_002458"
     CDS             complement(2126..2998)
                     /locus_tag="RD744_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0558429.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="RD744_002459"
     CDS             complement(3020..3940)
                     /locus_tag="RD744_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558430.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="RD744_002460"
     CDS             complement(3940..4209)
                     /locus_tag="RD744_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558431.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="RD744_002461"
     CDS             complement(4206..4586)
                     /locus_tag="RD744_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDU0558432.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1294 a         1062 c          800 g         1729 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatga
//
LOCUS       DAPCMU010000158         4825 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPCMU010000158 DAPCMU010000000
VERSION     DAPCMU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.163"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(207..1169)
                     /locus_tag="RD744_002462"
     CDS             complement(207..1169)
                     /locus_tag="RD744_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0558433.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="RD744_002463"
     CDS             complement(1171..2610)
                     /locus_tag="RD744_002463"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDU0558434.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="RD744_002464"
     CDS             2795..4741
                     /locus_tag="RD744_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDU0558435.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1543 a          823 c          791 g         1668 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttat
//
LOCUS       DAPCMU010000159         4813 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPCMU010000159 DAPCMU010000000
VERSION     DAPCMU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.164"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(59..2008)
                     /locus_tag="RD744_002465"
     CDS             complement(59..2008)
                     /locus_tag="RD744_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDU0558436.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="RD744_002466"
     CDS             complement(2050..3108)
                     /locus_tag="RD744_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDU0558437.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="RD744_002467"
     CDS             complement(3227..3682)
                     /locus_tag="RD744_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558438.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..>4813)
                     /locus_tag="RD744_002468"
     CDS             complement(4266..>4813)
                     /locus_tag="RD744_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558439.1"
                     /translation="LKALSDKLGTLTYRFEDSDVYEQLWLFQKFRIDKQKETVTIKAN
                     EEFEFILNSIGKNFTRFELDNFTKLRSSYTKELYRHLMQYRNKEWRNGYWVVKVEDFR
                     RALSIPSSYRMTNIDKRILEQAKEEFLAPDENGIQVFETFDYEKIYAKKGRRVDRLRF
                     TFSEPESNLPTISMHNWLEED"
BASE COUNT         1392 a          950 c          681 g         1790 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta aca
//
LOCUS       DAPCMU010000160         4806 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPCMU010000160 DAPCMU010000000
VERSION     DAPCMU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.165"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            184..681
                     /locus_tag="RD744_002469"
                     /pseudo
     CDS             184..681
                     /locus_tag="RD744_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405295.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(766..1362)
                     /locus_tag="RD744_002470"
     CDS             complement(766..1362)
                     /locus_tag="RD744_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0558440.1"
                     /translation="MDGFERRTEEKKKKVLRAAFDLMNTDTGIENLTIDAVAKKANVG
                     KTSIFKYFGSKEKLIHEVFIRSMDEMGEKAKKIMEKNASFEETLIAMSQNKIDYLEKI
                     NKQFYLDLMSYYTKKEDSGFTRMMEAYTKQSFGIMLDLFHRGRKEGKVDLKYSDEFLM
                     LYFQAMIEGISNPQIYEKILPYTAEWTELLVKGIAPRQ"
     gene            1673..2554
                     /locus_tag="RD744_002471"
     CDS             1673..2554
                     /locus_tag="RD744_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558441.1"
                     /translation="MTEIVKVQGLQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKS
                     TTIRTLLGIINRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFI
                     KLHGGGSKAKRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSG
                     LDPLMEAVFQEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELR
                     HLTRSTVTLVTKGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVIKI
                     ESVPPTLEDLFMRHYEG"
     gene            2578..4191
                     /locus_tag="RD744_002472"
     CDS             2578..4191
                     /locus_tag="RD744_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDU0558442.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(4263..>4806)
                     /locus_tag="RD744_002473"
     CDS             complement(4263..>4806)
                     /locus_tag="RD744_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558443.1"
                     /translation="ALAVIGSSAYNKIHERPSAANERLEIGNWEADTVAGKTGRARIV
                     TLVDRRSRFSLLGKVEKKISKNVVDCMINLLRNVGEDKCKTVTPDRGKEFSQHSRITL
                     ELNGTKVYFPDPHAPWQRGTNENTNGLLREYSPKGEDISGIFDSQIQEWANKLNTRPR
                     KCLNWKTPYEVFFNESLHLI"
BASE COUNT         1514 a          817 c          966 g         1509 t
ORIGIN      
        1 tggaaagctt gcagccacct acaccaccaa tacccaaaac acctaaactt ccaggtattt
       61 aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt aactactctt tttttataaa
      121 aacatttcct caaacgccta acaaaaatgg ttgtcaaaaa gccaaaaatc aaaaagaaaa
      181 ggaatgataa aaatgaaata cattaaaaac gttgaaacat tggaagaatt gaaaaaagcc
      241 tacaaaaagt tagctttgaa gttacacccc gattgtggag ggaatgaaga agaaatgaaa
      301 actttgaaca atgaatatga cgaactgttt agcaaactta aaaacactca taagaataaa
      361 gacggtgaaa cttacaccaa agaaacaaca gaaacacccg aacagtttaa ggatattatt
      421 aataaattgt tcaatctaaa aatggacggc gtagcaatcg aagtagtagg aacatttata
      481 tgactaacag gtaatacaaa accatataaa gaagatataa aagccctaga attccgatat
      541 tcacctaaaa aatatgcttg gtataaagcc cctaaagatt acaaaaaaag aagccgacta
      601 cgatcaatct gtgagaagcg gctgtctctg agacaattgc atcacatttt agttgtgaat
      661 atgtatctca aatacaccta gcagatgaat cgattctgat ttgtatgcat acattttatt
      721 tttttgtgga ttcggcgagt atttcttctt ttgttgttcg taaaactatt gtctcggagc
      781 gattcctttt accaacagct cagtccattc cgctgtataa ggcaggattt tctcataaat
      841 ctgcggatta gagatgcctt cgatcatggc ttgaaaatac aacatcaaaa attcatctga
      901 atattttaaa tcgacttttc cttctttacg cccgcgatgg aataaatcca acataatgcc
      961 aaaactttgc tttgtataag cttccatcat tcgggtaaag ccgctgtctt cttttttagt
     1021 gtaatagctc attaaatcta ggtaaaattg tttattgatt ttttcgaggt aatcgatttt
     1081 attttgactc atggcgatca aagtttcttc aaatgatgca tttttctcca tgattttttt
     1141 agctttttcc cccatttcat ccattgaccg gataaagact tcatgaatga gcttttcttt
     1201 gctgccaaaa tatttaaaaa tagaagtttt tcctacattg gcctttttgg ctacggcatc
     1261 gatcgttaaa ttctcgattc ccgtatctgt attcatcaga tcaaaagcag ctcgtaaaac
     1321 ttttttcttt ttttcttctg ttcttcgttc aaaaccatcc attaaaaata cctactttct
     1381 ataaaaaaga actttttgtt tatcatagtt caaaaatagc ttcgtatata ttatatagga
     1441 aataatacga actaacaatg gaatcatagg tttaaattta aaattatttt tattgactgt
     1501 taaaataaaa aagaatatta ttggagctgt aaaagaactt tggtttgaaa caaagtataa
     1561 aggggtattt tatgaacgat acgtattgta ttggttcata aaaaaggtgc gttttgttca
     1621 gactggaaag ctcgttataa aatcatgtca aaagaaaagg gagttatgta ccatgacaga
     1681 aattgtaaaa gtacaaggct tgcaaaaaaa atttggtaaa ttccaggcgt tgaaagatgt
     1741 ctcattcaca gtaaacgccg gtgaagttgt tggttttatc ggaccaaatg gagcaggaaa
     1801 gtcaacgacg attcgtacac tgctaggaat catcaaccga gacgaaggag atgtccaaat
     1861 attcggaaaa gatgtttgga aagatagtct agaaatccat aaacgaattt cgtatgttcc
     1921 tggggatgtt gctctttggg gcagcctgac tggtggagag atcattgatc tatttatcaa
     1981 acttcatggc ggcgggagca aagcaaagcg tgattattta atcaaacgat ttgaacttga
     2041 tccaaagaaa aaagccaaag gttactctaa aggaaatcgt caaaaagtcg gtttgattgc
     2101 tgcactttca gttgaatctg atctgtatat tttagatgaa ccgacttcag gactagatcc
     2161 attgatggaa gcagtattcc aagaagaagt agaaaaaatc aaaaatgatg gcaaagcgat
     2221 tctattatct tcacatattt taagtgaagt tgaacgatta gcagataaag tagcaatcat
     2281 tcgacgtgga gaagtagttg aaacaggtac attagatgaa ttgcgtcatt tgactcgctc
     2341 aacagttaca ttggtgacaa aaggcgatat tgagaaactt gcgacgctct ctggcgtgca
     2401 tgattttgtt caaaaagacg gcaaagcaac tttttctgct gacaatgaag cgatgaatac
     2461 gattctgacc gaggcaacca aattaggtgt gataaaaatc gaatctgtac cgccaacgct
     2521 cgaagattta ttcatgcgtc actacgaagg ctgattgtcg gaacggagga aaagaaaatg
     2581 aatgaaaaat ttgcgcgttg gaacgtattg ttcattcaat acgtgaaacg cgattggaaa
     2641 aaaataattg tttgggtttt aggtttgggt ttgttctcag gagcatttgt accagcattt
     2701 gaagagattg ctaaaggaca aggtctttta gggatgtttg aaacgatgca aaatccagcg
     2761 atgatctcga tggttggacc tacaccaatc aaaataggta cggattatac tttaggagcg
     2821 atgtatgctc aagagatgtt gctgttttgc ggattgttcg caatgattat ctcagcactt
     2881 catgtggtga gccacacgcg aaaagaagaa gaattaggtt tgactgaatt ggttcgctca
     2941 tttcgagtag gacgacaagc caattcatta gctgttatca gtgagatgct gttgatcaat
     3001 cttttattag gtcttttaat cggcggactc atgatgagtt ttggtgtaaa aacgattgat
     3061 gccgaaggag ctttcttgtt cggaggatca attgcattgg cgggaattat cggtggtgta
     3121 ttggcacttg tgatgtcgca gattatggcg acttctactg gagcaaccgg ctcgacatta
     3181 agtcttatag gacttttgta tatcgtgcgc gctggaacag atgtgtctaa tcttgattta
     3241 tcaatgttca atccaatggg atggatttac ttgacctatc ctttcacaga aaataactgg
     3301 ctaccattat tatttgcttt gatttttagt cttgttttta ccgtacttgc gtttgtgttg
     3361 gaagaacatc gcgacatggg cgcaggttat cttcctgaac gagaaggacg tgcgacggcg
     3421 aagaaatcac tactttctgt acctggtttg tttttcaaga ttaataaagg agtaatgatt
     3481 ggttggctga tcgcatttgt cgttatggga gctgcgtatg gctccattta tggagacatg
     3541 caagtctttc ttggcggaaa tgaactgatg aaacaaatgt tcactcaatc tggcgtttcc
     3601 attgaagaat cctttacggc aacgatcatg atggtaatga ttggattagt cacaatcttg
     3661 ccaatcgcgg tggtcaataa attatttgca gaagaaacaa gactgcatct gagtcaactg
     3721 tatgtaacga agattacgcg aggccaatta tattggacaa cgatattttt agctattttt
     3781 gctggagtcg taggtattgg cttagcatca gcgggattag gtggaacggc gatttctgcg
     3841 atgaaaaatg aatcgactat ggatctgacc gatttcttag ctgctggata caattttctc
     3901 ccttccatct tattttatat tggtttggct gctttagcgt taggctggtt gccaaaattt
     3961 ggaaaagtaa tctatgctta tctaggctat tcctttgctt tgaattattt cggcggaatc
     4021 ttagatttgc cggattggtt ctcaaaaact gcaattcaaa gttggattcc acgcttaccg
     4081 atggaaaaat ttgatggaac gatttttgca gtaattactg ttatcagtat cgtcttctta
     4141 tttgtcggct atttaggata caaacgccgt gatatggtag aaggcgctta aaaattgaat
     4201 ttctaaaaaa ccgttcatat ttacgaaagt cgatttgagc ggttttttta tggcttcaat
     4261 tgtcaaatta agtgcaacga ttcattaaaa aatacttcat aaggggtttt ccaatttaga
     4321 catttccgtg gtcgcgtgtt taatttattc gcccattctt gaatttgact atcgaaaata
     4381 cctgaaatat cttcaccttt gggtgaatat tctcgtaata aaccattagt attttcgttt
     4441 gtaccacgtt gccatggagc atgtggatct ggaaaataca ctttagtgcc atttaattct
     4501 agcgttattc gactatgttg ggagaattct tttcctctat cgggtgtaac ggttttacat
     4561 ttatcttctc ctacatttct tagtagattg atcatacaat caaccacatt tttagagatt
     4621 ttcttttcaa ccttaccaag tagggagaaa cgagatctac gatcgactag agttacaata
     4681 cgagccctac ctgttttacc ggctacagta tcagcttccc aattaccaat ctcaaggcgc
     4741 tcattggcag cagatggcct ttcatggatc ttattgtatg cagatgatcc aatgacagct
     4801 agggca
//
LOCUS       DAPCMU010000161         4788 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPCMU010000161 DAPCMU010000000
VERSION     DAPCMU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.166"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            564..989
                     /locus_tag="RD744_002474"
     CDS             564..989
                     /locus_tag="RD744_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558444.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="RD744_002475"
     CDS             1019..1183
                     /locus_tag="RD744_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558445.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="RD744_002476"
     CDS             2141..3118
                     /locus_tag="RD744_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558446.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="RD744_002477"
     CDS             3128..3625
                     /locus_tag="RD744_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558447.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="RD744_002478"
     CDS             4001..4282
                     /locus_tag="RD744_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558448.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="RD744_002479"
     CDS             4284..4700
                     /locus_tag="RD744_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558449.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAPCMU010000162         4737 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPCMU010000162 DAPCMU010000000
VERSION     DAPCMU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.167"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            72..1118
                     /locus_tag="RD744_002480"
     CDS             72..1118
                     /locus_tag="RD744_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0558450.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="RD744_002481"
     CDS             1135..2358
                     /locus_tag="RD744_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDU0558451.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="RD744_002482"
     CDS             2447..3334
                     /locus_tag="RD744_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDU0558452.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="RD744_002483"
     CDS             3420..3650
                     /locus_tag="RD744_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDU0558453.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="RD744_002484"
     CDS             complement(3707..4672)
                     /locus_tag="RD744_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDU0558454.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1602 a          788 c          963 g         1384 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agtttta
//
LOCUS       DAPCMU010000163         4727 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPCMU010000163 DAPCMU010000000
VERSION     DAPCMU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.168"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..290)
                     /locus_tag="RD744_002485"
     CDS             complement(<1..290)
                     /locus_tag="RD744_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDU0558455.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="RD744_002486"
     CDS             complement(283..624)
                     /locus_tag="RD744_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558456.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="RD744_002487"
     CDS             complement(798..1523)
                     /locus_tag="RD744_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDU0558457.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="RD744_002488"
     CDS             1576..2052
                     /locus_tag="RD744_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558458.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="RD744_002489"
     CDS             complement(2058..2240)
                     /locus_tag="RD744_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558459.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="RD744_002490"
     CDS             complement(2240..2539)
                     /locus_tag="RD744_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558460.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="RD744_002491"
     CDS             complement(2644..2847)
                     /locus_tag="RD744_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558461.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="RD744_002492"
     CDS             complement(2862..3608)
                     /locus_tag="RD744_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDU0558462.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="RD744_002493"
     CDS             3686..3994
                     /locus_tag="RD744_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558463.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="RD744_002494"
     CDS             complement(3965..4222)
                     /locus_tag="RD744_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDU0558464.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="RD744_002495"
     CDS             complement(4520..4660)
                     /locus_tag="RD744_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558465.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1290 a          927 c          743 g         1767 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactt
//
LOCUS       DAPCMU010000164         4710 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPCMU010000164 DAPCMU010000000
VERSION     DAPCMU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.169"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(209..439)
                     /locus_tag="RD744_002496"
     CDS             complement(209..439)
                     /locus_tag="RD744_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDU0558466.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(669..1487)
                     /locus_tag="RD744_002497"
     CDS             complement(669..1487)
                     /locus_tag="RD744_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDU0558467.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1648..2337
                     /locus_tag="RD744_002498"
     CDS             1648..2337
                     /locus_tag="RD744_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDU0558468.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2351..3853
                     /locus_tag="RD744_002499"
     CDS             2351..3853
                     /locus_tag="RD744_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0558469.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3866..4342
                     /locus_tag="RD744_002500"
     CDS             3866..4342
                     /locus_tag="RD744_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDU0558470.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4439..4549)
                     /locus_tag="RD744_002501"
                     /pseudo
     CDS             complement(4439..4549)
                     /locus_tag="RD744_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1588 a          673 c          830 g         1619 t
ORIGIN      
        1 tttacttcag atggtaaggc aacaaaataa aatacaatag gcagatgaac taataaataa
       61 aatctgtatt aaatacagat aaattaatat aaaaatcaaa aggatcataa acttttaaca
      121 agtgatgaaa tgggtggaaa ttgattctta tagacagttt gtctcataac attgaagtat
      181 tttgcaattt tagcattgaa acactttttt aatcttcaac gttgttctta taaacaagat
      241 gattgggaat cttttgaatt aacactgtat agcttagaac accctttata gaaaacattc
      301 gcttagcaat cttaataatt ccttgtgccc gatgtcgttt agattcagta ggttcctgct
      361 catcgatcat ggaaaaaatt tgaaggatca gatccttaat aaatttattt aagagaggat
      421 gatccattgc ttcatccata ctcgataaac tagtgataat gagctacacg tttttctttc
      481 aagttgatca atagcttcca cgataaaaat atctttagat aaaatcaaga accgtaggaa
      541 ctacagggat atttggtcaa taagagccac cgctattagt aaaaaataag ctatcaagta
      601 tgctctcatc ccaagaattg cctacttcaa tcgttaaaaa ccgttaggtt tagctaagtg
      661 ggggtagttc actcaatact tttatcaata atagagcgca aaactttttc tcttaattca
      721 actgaatcaa tattattgtt ttgttcataa ccatgaacta atagatcaca aatataaagt
      781 tgtgatattt ttccagctaa agatccacca ttaagaaatt cttcaatggc tgtttgtaaa
      841 actaagtctg cagactttcc tataggtgaa tgaatatagt tagttatagc aataattttt
      901 gcgccatttt ttttagctat tttcaatgaa tcataagtat ctttcgtttt cccagataaa
      961 gaaaaaatga ttactaaatc attaactgtt aataaagaag cgacttgggc ttgaaaatga
     1021 gggtctagta cagcttttgc ttgaactcct acgcgaagaa acatattttc taaatcaaga
     1081 ctagtatttc cactagatcc cactccaaat atatatatgt tttttgattg agtgatcatt
     1141 ttaatggctt cattcaattt ttcttcgtct aaaagcctaa tagtggaata gagagcttct
     1201 gttaaactat tagcaatttc atcatagtac tttacactag tcggatgttc ttttttttga
     1261 ctaaagtctt cctttgcaat ttcaattttt aaatcagaaa aaccagaaaa accaattttt
     1321 tgacaaaagc gaataatagt agcatcgcca acttttgtgt ttttcttaat atcactcatc
     1381 gtactataaa taatagtttt cccagaattt aaaataaaat cagcgacttt tttttcagac
     1441 ttcgttaaaa atggataatt ttctttaatt aaatagatat aactcataca aactcctcta
     1501 caatttcaac ttttctccat tatactccaa aataataaaa aaaactccta aatactccat
     1561 ttttatcttt acttttatga aagcggatgc tatattgttt ttggagttgt tatccaaaaa
     1621 taataaaaaa ttggagtgtt taggataatg gataaaaaga ggtttttaga tcttatttca
     1681 aaaggtttga ttgtttcctg tcaagcatta cctggtgaac cattttacaa agaggaagaa
     1741 agtttaatgc cttattttgc tttggctgct caaagggctg gagcagttgg tatacgtgca
     1801 aattctgttc gagatattat tgaaattaga aagtgtgtat cattaccaat gattggaatt
     1861 attaaaagag attatccacc agaatctcca ttcattactg ccactatgag agaagtagat
     1921 gaattagtag aaaccggtgt tgaggtaatc gctcttgatt gcactcttag aaatcgacat
     1981 gatggactat ctataaattc ttttattact aaaataaaag aaaaatatcc tcatcagtta
     2041 tttatggcag atatttctac atttgaagaa ggcaaaaatg catataaagc aggaatagat
     2101 tttgtaggaa ctactttaag tggatatact gaggaaagta aaaaacaaga tgggccagat
     2161 atagatttga ttaaaaaatt ggtagaagaa gggatttgtg taattgctga gggaaggatt
     2221 catactccag aacaagcaaa agagataaaa aaaataggag ttgcaggtat tgttgtgggt
     2281 ggtgctatta ctcgaccaca ggaaattgca tcaagatttg ttaaagcatt aagttagggg
     2341 gagaaataaa atgtttaaaa aattttcaca attaggacgc gctttcatgt taccaattgc
     2401 tatattacca gtagccggat tactattggg actgggagga gcgttaacaa atgagagtgc
     2461 tataaatgct tatccatttt tagatcaacc gtggttgcat acaattttaa gtattatgag
     2521 ttatgctgga aatgcagttt ttgcaaacct agcactaatt tttgcaattg gtattgctgt
     2581 aggacttgca aatggagata aaggaacagc tggtttagct ggtggagtag cttatctagt
     2641 atatactgca actattagtg ggtttcttga acttttttca gcaaaagatg caacactaga
     2701 tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt gcatttttac acaatcgtta
     2761 tagaaaaatt gaattaccac aattcttagg tttttttgga ggatcaagat ttattccgat
     2821 tgtttctagt ttagctgcaa tcattattgg ttctttcttt tatttaattt ggccaccaat
     2881 tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa atgggtagtt taggaacttt
     2941 tttatatggt tttttattgc gtttaacagg tgctgttgga ctacaccaca ccatttaccc
     3001 tttgttctgg tatacatctt taggtggtac cgaaactgta gctggatcaa caattgctgg
     3061 agcccaaaat attttctttg cccaactagc tgatccaaac cacactggtc tgtttactta
     3121 tggtactaga ttttttgcag gaagatttgc aacaatgatg tttggattac cagcagcatg
     3181 ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa aagaatggtg ggctttattt
     3241 tagcggggcc cttacatcat ttttaactgg aattactgaa ccagttgaat atatgttttt
     3301 atttgttgct ccttggcttt atgtgattca tgcattctta gatggattat ctttttattt
     3361 tgctgatatc ttaaatattc gtgtagggaa ttccttttca ggtggtttga tcgacttttt
     3421 attattcggt gtacttcaag ggaatgacaa aactaattgg ttaaaattaa ttccttttgg
     3481 tattgcatgg gcatttatat atttctttgt ttttacattc tttatcaaaa aatttaaggt
     3541 agctattcct ggaatggaag aagaatcaga agttcattct gaaaaagtaa taaacaaaaa
     3601 tacatcatta catgaagagt ctcttatcat tattgaagct cttggtggaa aagaaaatat
     3661 tgagtctgtt gaagcttgtg caactcgttt gcgtgttgct gttacgaatg gggagattat
     3721 tcaaaaacaa acaataaaag acactggtgc aactgctgtc ttcgaagtga agggtggcat
     3781 ccaagctgtt tttggaggga aagctgactt attaagtcaa gaaatcaatc aaatattagg
     3841 aaaagataat taggaggttt ttaatatgtt tgaaattttc aaaaagaaaa aacaagaaac
     3901 tttttatgca ccttgttccg gaaaaatcat ttccatcaca gaagtaaatg atcctgtctt
     3961 ttcacaaaaa ttactaggtg atggttttgc cgtcatacct gatggttcaa aaatttatgc
     4021 tccattggct ggtcgagtaa caaatatatt tcctactaaa catgctatta atataaaaag
     4081 caactcaggc attgattatt tgattcatat tggaattgat acagttgaat tacaaggaga
     4141 gcctttttcg atttgtgttc aagaaaatga tgaggttact cctgaaacct cgttggttga
     4201 tgtggatttt gacaaaataa taaaatctgg taaagatact tctgttatag ttgtttttcc
     4261 ggagaaaaat cagttcaatg atttaataat agataatggt cgaattgctc atgggaattc
     4321 atgtggaaaa atcacttttt aaaaaagtgt atataaaaag ctataaaata agctaaatag
     4381 gatatttgtc aaatgaagtt ggtgaacaga aagaactgtt ttctttctgt tcattttttt
     4441 attttattag taatgattcc ccatgccaaa tcatgatccg taatttaaag ttataaaagc
     4501 ttcgaaatcc attggcattt cgttggaata ccttgatatg attattcaaa tataaaatat
     4561 agagagaaga gagagaggga agatatttat aaagggcaaa gttatgttta ctcaacttag
     4621 gaaatagaaa tagatcaatt tatattctct tgtcaatcga atatgaacct tagcactata
     4681 taccaaatat agtaaaatgc tgaaagatgt
//
LOCUS       DAPCMU010000165         4634 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPCMU010000165 DAPCMU010000000
VERSION     DAPCMU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.170"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            174..518
                     /locus_tag="RD744_002502"
     CDS             174..518
                     /locus_tag="RD744_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDU0558471.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="RD744_002503"
     CDS             519..938
                     /locus_tag="RD744_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDU0558472.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="RD744_002504"
     CDS             complement(1030..1380)
                     /locus_tag="RD744_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558473.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="RD744_002505"
     CDS             complement(1346..2566)
                     /locus_tag="RD744_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDU0558474.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="RD744_002506"
     CDS             2932..3498
                     /locus_tag="RD744_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDU0558475.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVPVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="RD744_002507"
     tRNA            3643..3730
                     /locus_tag="RD744_002507"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="RD744_002508"
     CDS             4064..>4634
                     /locus_tag="RD744_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDU0558476.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          779 c          890 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtac cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DAPCMU010000166         4603 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPCMU010000166 DAPCMU010000000
VERSION     DAPCMU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.171"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..501
                     /locus_tag="RD744_002509"
     CDS             <1..501
                     /locus_tag="RD744_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HDU0558477.1"
                     /translation="NIEKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKT
                     DIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNII
                     NANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWI
                     QKQIAY"
     gene            474..1151
                     /locus_tag="RD744_002510"
     CDS             474..1151
                     /locus_tag="RD744_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDU0558478.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1141..2283
                     /locus_tag="RD744_002511"
     CDS             1141..2283
                     /locus_tag="RD744_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDU0558479.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2296..3273
                     /locus_tag="RD744_002512"
     CDS             2296..3273
                     /locus_tag="RD744_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDU0558480.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3273..4181
                     /locus_tag="RD744_002513"
     CDS             3273..4181
                     /locus_tag="RD744_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDU0558481.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVTTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4182..>4603
                     /locus_tag="RD744_002514"
     CDS             4182..>4603
                     /locus_tag="RD744_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDU0558482.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFS"
BASE COUNT         1738 a          557 c          853 g         1455 t
ORIGIN      
        1 aatatagaaa aacaaatttt agctttgatc aattcaatgg aacaacaatc tcaaccaatt
       61 atttgcattt tacctaatag tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa
      121 tatttaaaaa cggatattat ttatattacg aatttagagc cggaggaata cctgactata
      181 ttaaaagagt gcaaatttat gatcggaaat tcttcttctg ggataattga agctgcatca
      241 ttcaaaatac cagtaataaa tttaggaacc agacaaaatg gacgagaaca atctaaaaat
      301 attataaatg caaatctaaa tgaaaatgaa attgaaactg ctttgaataa aatacaagat
      361 cctcagttta taaaaagctt gaaaaatgtt aagaacatct acggtgacgg caacagttct
      421 agaagaataa caaaaacatt gaatcaaata ttgaatgatg gggaagatat taagtggatt
      481 caaaaacaaa tagcttatta aaatatacta gtgatccata cattattatt ggggctggcg
      541 gacacgcaaa agtcattatt gaaaccttaa gattagaaaa taagttaata tatggatttt
      601 ttgatgataa gcaaaataaa atgcaagatc tgaaatatct tgggaaaata aacgaattga
      661 atagctgggc ggatagcaaa aaaaaaatct ataaatttat tgtagctatt ggtgactgtg
      721 tagtaagaaa aaagattata aaaaaattga atatgccaag tgagcaatat ggaatagctg
      781 ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg ttatggaact gttatttttg
      841 cgaatgctgt aattaaccca gatgctgtaa ttggagagca tgctataata aacacaggta
      901 gcattgttga gcatgattgt agaattaata actatgttca tttatctcca ggtgtatgct
      961 tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg aattggttct caatgtatcc
     1021 agatgaagaa aattggatct tggtgtatga taggcgcagg gagcactatt gttaaggata
     1081 ttccctcaca ttccttagct tatggcaatc cagctaaaat taagaaagaa ggaattaatt
     1141 ttgaattcta gaattttatt atcaagtcct catatgggag gaacagaaga aaaatatgtt
     1201 gctaaggctt ttaaagataa ttggatagcg ccattaggtg aaaatgtgga taaatttgaa
     1261 gaaagtatta caaaatatac tggttctaag ggcggagctt ctgcgttaag ttcaggtaca
     1321 gctgccatac atcttgcctt aagactgctt aatgttggaa aaaacgatat tgtctttgct
     1381 ccttcattta cttttatagc tacagtaaat cctattttat atcaaggggc tgtaccagtg
     1441 ttcattgact cagaacctaa tacatggaat atgagccctg ttgcactaga gagcgcatta
     1501 gaagaagctg aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa tttatatggt
     1561 caaatggctg attatgaacg aattttagct attgcaaacc aatttaatgt tccagtgata
     1621 gaagatgcag cggaatcgtt aggttctact tataagggaa tttcgtcagg cttatttggg
     1681 acaattggga tctattcatt taacggtaat aagatcatca caacctcagg tggaggagct
     1741 ctagtgtcta gtgataaaaa atatgttgag cattcaagat ttttagcaac acaagcaaga
     1801 gatcaagcac cgtattatca acatagcgaa ataggttata attaccgtct aagcaatatt
     1861 tctgccggta tcggacgtgg acaaatggaa gtattgaatg atagagtagc taaacgaaga
     1921 gaaattttca atcgatataa ggaagctttt tctggaattg atagaattga aatgatgcca
     1981 gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa tgattataat
     2041 tctattgatc aagtagttaa cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga
     2101 gctttgtgga aaccactaca tttgcaacca gtatttcaag gggtaaaaat gtattaccat
     2161 gataataatc ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt accttctgga
     2221 agtaatatga cagatagtga acaagaattt gttattgatg agtttaaaaa aatattcaag
     2281 taggtgtgat attttatgaa cataggaatt attggtttag gttccatggg gaaaagaaga
     2341 cttagactca ttcaagaaca ctttaatgat attttactat gtggtgttga tactaatgag
     2401 gcgagacgaa aggaagttga aaaggaattt ggaataccaa cttataatag tctaacagag
     2461 gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta catcccctat aacccatgaa
     2521 tttattataa aagaggcttt aaatttagat tgtaatatct ttacggaaat caatttatta
     2581 aatgaatatt atgatgaaat aatcgaagta gctaaaaaga aagatagatt actttatcta
     2641 tcttctacat ttttgaagag aaaagaaatt caatatatta agtctaaagt gacgcataat
     2701 aaaaaaattt cttatcaata ccatgtagga cagtatttac ccgactggca tccttgggaa
     2761 gattatcgca atttttttgt ttcaaacaaa aaaactaatg ggtgtagaga aatattcgct
     2821 attgaattac cttggattat agatacattt gggaaagtta aatcgtttac tgtaatgacc
     2881 cagaaattat ccaatcttga tattgattat tcggacagtt tcactcttat tattgagcat
     2941 gaaactggta tagttggaac tttaagtgta gatgttgttt caagagtagc taaaagaaat
     3001 ttaactataa ttggtgaaaa gattttaata gaatgggaag gtaacccgga ttctctattt
     3061 tcatggaatg aagaaacttc tagtatgaca gatattcaat tgtacgatga ggttatgcat
     3121 gataataatt attcacatac tattattgaa gatgcatatt tagaggagat taaagaattt
     3181 ttcacgcttt tggaaaagaa aatggaaccc caatatactt ttgaaaaaga taagtatatt
     3241 attaacttaa ttaacgacat tgaggtaaat taatgatgaa agtaattttt tttgggttag
     3301 ggtcaattgg aaagagacat ctaaaaaatc taataagcta tggaaaaaag aattctatta
     3361 attttgatat aacagcttat agtagcagtg gaaaaaaaga gtttgaagaa ataacgtata
     3421 ttcaaaatag agacgatttg gatacacatt atgatatagc ttttattaca aatccgactt
     3481 ttaagcatct ggaaacacta aatgtaattg ttgaaaaagt tgattacata tttttagaaa
     3541 aacctgcttt ttctgagagt attgatctta ctcaatataa ttatgattac aaaaatattt
     3601 atgttgctgc tccactgagg tataaaaata tcatggaagt tgttcgtaat agtattcaaa
     3661 gtaaaaaagt atattctgta agagctattt gttctactta tcttcctgat tggagaaaag
     3721 gtactgatta tagaaataat tattctgcct ttaaatctat ggggggagga gtagaattag
     3781 actgtataca tgaattggat tatattataa acctatttgg tttccctaaa tcagtaaaaa
     3841 aacagtttgg taaatattcg aatcttgaaa ttaacagtaa tgatttagca acatatatat
     3901 tgagatacga tgataaaatt atagaaattc atttagatta ttttggaaga gttactacaa
     3961 gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta aataataaaa
     4021 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
     4081 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
     4141 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
     4201 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
     4261 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
     4321 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
     4381 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
     4441 aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt
     4501 taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa
     4561 tagatgaaca tctaaaaaat ttagataaag acgtctactt ttc
//
LOCUS       DAPCMU010000167         4593 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPCMU010000167 DAPCMU010000000
VERSION     DAPCMU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.172"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            56..1990
                     /locus_tag="RD744_002515"
     CDS             56..1990
                     /locus_tag="RD744_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDU0558483.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2447..2863
                     /locus_tag="RD744_002516"
     CDS             2447..2863
                     /locus_tag="RD744_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDU0558484.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2877..3443
                     /locus_tag="RD744_002517"
     CDS             2877..3443
                     /locus_tag="RD744_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDU0558485.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3466..4452
                     /locus_tag="RD744_002518"
     CDS             3466..4452
                     /locus_tag="RD744_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDU0558486.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         1427 a          856 c         1010 g         1300 t
ORIGIN      
        1 taaaagaatt atcatatatt cattatatag atagtctata aggagcggat gatcaatggc
       61 aaagcaaaat attgtcgttg taggagcagg atatgccggt gtttcagcta caaaatactt
      121 ggcaaagaaa ctgaaaaaag aagatgtagc aattactctg atcgatcgtc attcgtacca
      181 tactatgatg accgagttgc atgaagtagc tggcggacgt gtagaaccag aagcaatcca
      241 atacgatctg cagcatttgt ttgctcgtca aaagaatgta caacttgtca cagacactgt
      301 aatcggtatc gataaagaac gaaaaatcgt caagaccaaa ttaggttcct atccttttga
      361 tcagctgatc cttggaatgg gcggagagcc gaatgacttt ggtacacctg gtgtcaaagc
      421 acacggattc accctttggt catttgaaga ttctgtaagg attcgggaac atattgagaa
      481 gacagtagca aaagctgcat tggaaccaga tgatgaactg cgtaaagcca tgctgacttt
      541 cgttgtctgt ggttctggat ttaccggtat tgaaatgatt ggggaactga tcgactggaa
      601 aaatcgccta gccaaagatt ataaattaga tccaaacgaa atcacactga tggtcgtaga
      661 ggcaatgccg acgatcttga atatgttgga tcgaaatgat gctgcaaaag ctgaacgcta
      721 tttgcgtaag cacaacgtgg aattattatt gaatgcacca atcgtagaag tggctgctga
      781 tcatatcaaa ttgaaagacg gacgcaacat tccgactcac acactgattt ggacagctgg
      841 tgtcaaagga acttctgacg ctgctgactt tggattagaa tcagcacgcg gacagcggtt
      901 gatcgccaat gaatatatgc aggcaaaagg ctacgaagat aaaaacatct atatcattgg
      961 tgatttagtc ttttatgaag aattcccagg aacaccgaca ccgcagatcg ttcaagctgc
     1021 tgaacaaaca ggacacacag ccgcagcgaa tatcgtggct tcgatcaaag gcggagaaaa
     1081 gcataaattt aaaggaaact atcaaggatt tatggtttct attggtgcca aatggggggt
     1141 agctaaccta tttgataaga tccatttaag cggattttta gctatcgtaa tgaaacatat
     1201 cgtgaatttg aaatacttct ttgatattcg ttctggttat tatatgttcc aatatttaat
     1261 gcatgagttt ttccatatca aagatgatcg caacgtgact cgaggacatt cttctcgtta
     1321 cggcaatgtc ttatggagtg tgccacttcg tattttctat ggtttagttt ggttcgttga
     1381 agcttccaaa aaaatcattg ggaatggtga agtgttcaaa ccaagtactt ggtttggtga
     1441 aggatcatgg ttcactggaa acagtgtgtt cccttggaca tgggaataca atgtaacaga
     1501 tgttgccagt ggtgcttctg ctgctggtga tgcaaccaca gctgccagcg gagcaggggc
     1561 aagtgccgga gaagcagcga ctcaagctgc acattttggt ttgagttatg catacggtga
     1621 agaaccaatg gctgtgattg agaagtcacc agactggttc acaaagatca tgcaagtgat
     1681 catgccaaac aatgatgtag caatgttctt ccaaaaattc atggtattct ttgaaattgc
     1741 cttggcatta gcattgattg ctggattatt cacatggcta tgcagtgcga caacgattgt
     1801 attggtgatc acgttctgct tatcaggcat gttttactgg gtaaacattt ggttcatttt
     1861 tgttgctttc gcattgatga acggttccgg acgtgcgatt gggttggatc gctgggtaat
     1921 tccatggcta caacgtacac taggtaattg gtggtacggt agaccaagtt cccagtacgg
     1981 caagaaataa atagataaag aacgttattt gaatgcaaga tttcaataga aaggggctga
     2041 gacaaaccgt cacagcctcc tttttatgta aaattaaagg aataagatgc ccgagataag
     2101 cctccatgag caagacacag gtgtcacaac ctctgttaca acctcttgtt ataaacggtg
     2161 ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaacttg aaaagtaatt
     2221 ttcgtaaatt ccttcttatt tcttgaagtt gcccacttct gtcacaacct cttgttataa
     2281 acggtgttcc acctgctgct tctcggtaat aagcccaaag aattagaaaa catggaaagc
     2341 tgttttctat tcttcgttct tattactcaa agcaagacac aggtgtcaca acctcttgtt
     2401 tttatgctaa aattgggtga gttaatctgg aaaaaggaga acaaaaatga gccttaaaga
     2461 atttgctgaa aaaagtctgc tcaaaccttg ggatgttttt attattttgc ttttgactgt
     2521 tctttctttt ttaccagtca tgatttttgg tcttcagcaa aacaccgcaa cagcagaaaa
     2581 agaagcggta ctgcgtgtgg atggcacaga aatcaaaaca ttccctttga tggctggaga
     2641 agagagcaac acatatactt acaaagatga acacggagat tataacttga tcgagataga
     2701 tggcgatcga gtacggataa aagaagcaga ttgcgatgac cagatctgcg tacgtcgagg
     2761 ctgggcaacg aaaaatggcg aaaccatcgt ctgtttgcca cataaattag tgatcgaagt
     2821 ccgttcaaca gatggaggtg acgaagacag tctgatttat tagaccttca cacaccatga
     2881 gtaaattaca aaaaatgatt tatatttcgc tacttgttgc acaaggagtg atcattggtt
     2941 tattggaaaa tatgatccct tatccttttt cttttgcgcc tggtgcaaaa ctaggcttag
     3001 ccaatctaat cacgatcgtt gccattttta ccatgaaaaa acaagacagc ttcttactac
     3061 tttggctacg actattcctg actactttgc taggcggaac gatttccaca ttcctttata
     3121 gtatgagtgg tgctttgctt agttattttg gaatgttatt ggtcaagcaa ttaggaccaa
     3181 aacgtgtcag tatcatcgga attagtgcgg ccggcggttt catgcataat gtcggacagc
     3241 ttttgatggc ttctttcatt gcacaatcat ggacagtcat gttgtacttg ccagtccttt
     3301 cagttttagg gattttatca gggattgcaa tcgggatcgc agcgaattat cttttacgac
     3361 atgtgcagac acttcaacga ttccaaaaag attatgagca gggaaccaaa caaaaatgga
     3421 gcccaatgtt taccaaaaaa taatcgtcac acaaggagtt tttttatgaa gctacatcca
     3481 atgtgggaaa actatcctgc tttaagtaaa gaattgacag aaacactaaa acggatggaa
     3541 gaaactgtcc gcctgaaaaa caaacctgtc gaacaagcca tcaaagaaac gatccatgcc
     3601 ggcggaaagc tgttgcgtcc tgcctatcag ctattgttct cacaatttgg accagagcaa
     3661 gatcgagaca aagccatcgc tttggcagct tctatcgaaa tgctccatac ggccacactg
     3721 atccatgatg atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa tatccgtagt
     3781 cagtttggta atgtcgtttc tgtctatgca ggagactatt tatttgtctg ctgttttaaa
     3841 ctattgtctg attactccac ttccttgaaa agtctccaat tgaattctcg cagtatggaa
     3901 aaagtattga atggcgaatt agggcagatg gatgatcgct ataattatga actttctgta
     3961 aaagaatatt tggaaaatat ttcaggtaag acagctgaac tgttccaatt aagttgttct
     4021 gttggcgctt ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg agatatcgga
     4081 ctttctatcg ggatggcctt tcagattatc gatgatattt tagattatac aaaagagagc
     4141 caggaaatcg gcaaacctgt actagaagac atgcgtcaag gggtctattc tttaccgttg
     4201 atctactctt tgcaaaaaaa caaaccaaag ctactacctt atttagagaa gaaagcggca
     4261 atgacagaag aagatgtcga cgccgtacgc aaaatcgttg aacatacagg aggcgtggaa
     4321 gaagctagga agttagcggc ctcttacaca gaaaaggctt tgaaggaaat aaagaaatta
     4381 cctgccacct ctcttcgaac aaaagagaat ctcttttccc ttacccaact gattttggat
     4441 cgcaaagact gaatagggaa aggttcgata cgtaaataaa tttctcagaa ggaaatctac
     4501 gtgttttttg acaaattgaa ttgtagttct tgtatttatg ataaaaagca aaaataaaga
     4561 gcaacgtccg ttagcgacgt tgctcttttt tat
//
LOCUS       DAPCMU010000168         4537 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPCMU010000168 DAPCMU010000000
VERSION     DAPCMU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.173"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            256..1023
                     /locus_tag="RD744_002519"
     CDS             256..1023
                     /locus_tag="RD744_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDU0558487.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1028..2007
                     /locus_tag="RD744_002520"
                     /pseudo
     CDS             1028..2007
                     /locus_tag="RD744_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2158..2490)
                     /locus_tag="RD744_002521"
     CDS             complement(2158..2490)
                     /locus_tag="RD744_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HDU0558488.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(2584..3129)
                     /locus_tag="RD744_002522"
     CDS             complement(2584..3129)
                     /locus_tag="RD744_002522"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013311.1,HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558489.1"
                     /translation="MELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVA
                     ELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTFKRLWINSLEKDVIRSGFQN
                     LQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLI
                     FQRQEAIENFKKEPFFEVEAK"
     gene            complement(3386..3523)
                     /locus_tag="RD744_002523"
     CDS             complement(3386..3523)
                     /locus_tag="RD744_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558490.1"
                     /translation="MNSICLHALAILNNSLESDYGKALYAQCKIDVETIFDRLKGVFG
                     M"
     gene            complement(3527..3982)
                     /locus_tag="RD744_002524"
     CDS             complement(3527..3982)
                     /locus_tag="RD744_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0558491.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
BASE COUNT         1467 a          815 c          754 g         1501 t
ORIGIN      
        1 gtgaaatatc tattcaagta ttacaaaagt cttttacttt ctataactta ctgattaaga
       61 ggtcctactt tattttcgtc ttatacaaaa tctgacctaa gctaatatac gtcaatcctc
      121 tgttcttatt tcatcatcta acatttgttt ttgtttgaaa ttgtttgttt taccttgaaa
      181 atattgtctt ttatgataca attaaaagag aattatcttt ggaaaaaaat tactttatga
      241 aagaagggtc ttcatatgaa caaaaaacga cgattagaaa aaattttaga tatgttaaag
      301 attgatggga ccataaccat aaaagaaata atagatgaac tagatatttc cgatatgaca
      361 gcccgtagag accttgatgc tctagaagct gatggacttt taacacgtac tcatggtggt
      421 gcacaattgc tttcctctaa aaagccactt gaaaagacac atatcgagaa gaaaagtcta
      481 aatacaaaag aaaaaattga cattgctaaa aaagcctgct ctttaatcaa agatggcgat
      541 actattttta ttggacctgg aactacactt gaacaactgg cattagaatt gaaaggtcgt
      601 aaaggttata aaattcgtgt cattacaaat agtctccctg tgttcttgat tctaaatgat
      661 agcgaaacca ttgatttatt gcttcttggc ggtgaatata gagaaataac tggagctttt
      721 gtaggttcaa tggcttcgac aaatttaaaa gcaattagat ttgccaaagc ttttgttagt
      781 gcaaatgctg ttacccataa ttctattgct acatatagtg acaaggaagg tgtgattcaa
      841 caacttgccc taaacaatgc tgtagaaaaa ttcttattag tagacagtac taaattcgat
      901 cgatacgatt tctttaactt ctataatcta gatcaactcg ataccatcat tacagataac
      961 cagattagcc ctcaacactt agaggaattt agccagtaca ctactatttt aaaagcggac
     1021 tagaattatg acttataaaa atattgtttt tgaccttgat gacactcttt atgaccatca
     1081 actccccttt aaacgtagcg ttgagaaatg ttttccaacg attgatatcc aacaaataga
     1141 taacatttac aaacgtttca gatattggtc tgatgttgct tttcctaaat acacgaaaaa
     1201 actcatcagt attgagcagc ttagaatttt tcgctgtcag aagactatgg aagaatttgg
     1261 catcgatagt atttctagga cagaagctct agacttccaa gctgactatg aatatgaact
     1321 cgatcagatt acgatgattc ctgagataca tcaactactg ctggctctac ataaaaaccg
     1381 catcccaata ggtattctaa ctaatggccc tgttgactta cagagtcgaa actccaaaat
     1441 attggtgcct atcaatattt tgaaaagcaa aatatcatca ttagtcagtc cactcacgga
     1501 ggagctgcag aaaatagcta tgaaggtaat gtaaaaaact tacaaagtta ccgaaatgtc
     1561 caattatcta aaagggctat agaaattctt aaagaggaaa ttgagttaaa tcaccaacat
     1621 atgagattca atcctgatta taaagataat ggatggattt ttacttccaa aagtcgtcat
     1681 aaacccgact ataatggaac acctcttcat tatagtgtcc ttaacaattt cctcaactca
     1741 cctgaaaatg gaaaattaaa tcgaaaaggt aatccaaaac gagcaggaat tgatattgat
     1801 aataaactta gttttaataa acatattacg acccatatat tccgtcatac gcatattagt
     1861 ttcttagcag agtaaggaat acccctagaa gcaattcaag accgtgtagg gcattctaga
     1921 ggtagtcgtg taacagaaat ttatttgcac atcactaaaa aaacaaagta tcaaattata
     1981 cctatccttg atactcttac acaataagtg tggcaaatct gtggcaaaac ataagaaaaa
     2041 ggcttgtcca tattggacaa accctttatt ttcaagctta tataacctaa ttaagcttta
     2101 ccagctgatt agaataggat gttgcatgtt ttactacgtc tttttcatag agtttcacta
     2161 attcgtcaaa atttgggtgc tgttgtttga acgtatcatt atcaatgata acaacatttc
     2221 aagatcaagt cctaaaaaac cgctatattt gccaaaattc gcttttaaat aagcaaattc
     2281 gttctctgta ataaatggtg tatctgttct tggataactc aataattttg cttcatacag
     2341 tccttgcata gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga
     2401 ttgcaaacta cttaaagaaa acgaactcgg actattcgtt ttcttctctt tggtttgaac
     2461 atcagcaatt atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa
     2521 aaagctcctc ttgggtttta aaacgctgtg tggggcttag aacgccctta aatgaccctt
     2581 gttttactta gcttccacct cgaaaaaagg ttctttttta aaattctcta tggcttcctg
     2641 gcgctgaaaa ataaggtata aggtgggcgt ttgaacacgt cctagtgaaa atgtaccttg
     2701 tacgcccttc tgttgtaaat ttaacgtata caaagggctt gcgttcatgc cgatcaacca
     2761 atcggcaatt tggcgtgttt gcgcttcttg ataaaaggga tagtaattca ttcctggttg
     2821 caaattttga aaaccgcttc ggatcacatc tttttctaag ctattgatcc atagtctttt
     2881 aaatgtttta tcttttgaaa aggcatttgc attatggata atcgaccagg cgatattttc
     2941 accttctctg tcgctatctg ttgcaacaat aattgtattt gcctttttga gaagttctgc
     3001 aacaatttta aactgctttc ccttatcttt tgcaacttca aaatcgtatc gatcaggaaa
     3061 aatcggcaaa gattcaagtt tccaattttg ccacttttcg tcataatgac ctggttctgc
     3121 taattccact aaatgcccaa aaccaaaggt gataaacgtt tcatctgtaa atagtgggtc
     3181 tagcaaataa ctagctctaa atataaaaaa cgcgatgaat aatcataaat gtgattgctt
     3241 cgtcgcgttt tttatttttg gctgctttgt gttgaaaagc ttccgctttt cttctagcct
     3301 caagggataa tttggttaga ttcatgctca taagcatcat tccaatgtca gtaggtgcaa
     3361 cgttcttgcc tctgacatgg gttcttcaca taccaaatac acctttcaat cgatcaaaaa
     3421 tagtttctac gtcaattttg cattgagcgt agagggcttt tccataatca ctttcaaggc
     3481 tattatttaa aatcgctaag gcgtgaaggc aaatgctgtt catatctcaa aacggttttc
     3541 tgactcgcaa ttttttctct ttgtcttcca agttgattcc taagagatca ccaacatgcg
     3601 ccatcacctt atgaaactgc ttctcagtga tatgaagatc ataatgggct gtcgagggaa
     3661 ataaccactc agaattgata ttttcttggg ccagccaatc atgatattgc aacaagtctt
     3721 gctgaacagg ctttaaatat aacgtgtttg ctttaccggt ctttttatca tgaataaagg
     3781 ttgcattttt gacagagccg tctggattat agacatctga tttttttaat gtcatgacat
     3841 cactcacacg tagtagcgtg gccttaccaa cttgaaaaac ggtgtaattt cggcgaccag
     3901 cacggaaact atcgagaagt gtatcttgta ccatctttaa gacgctggag tctttgatgg
     3961 gtaggacaat ttgttgcacc atgattcatg tatacctttc tgaaattaaa taattaaata
     4021 aataattaat taaattgttt atttattgat gaccaggacc gtatttctaa acttctttta
     4081 tggtttatta tatcacttaa caaaaaaaca gcccccatta tctagtcact agataatggg
     4141 ggctgtttta atcagttttc aaaggggcga ttttctaatt atagaccctg agttaaggaa
     4201 tgtactgatg ttttctagta tagtccctag tttgataata tttattgtat ttttctgtat
     4261 ttagttattc gatgatatcg aggctaatat gtcagtcttc caagcagcca ttggttggta
     4321 tatcaatttt tataatcgca atcgtatctc aaatgttgcc taagtaactg aataaaaata
     4381 gtccaattta ttgacttctg agcagccgct gaagctatta aattattgaa aggaatcagc
     4441 cttttaaaaa gtcaacaaag aaattctgct ttaaatcata ttagggtatc acaaatactt
     4501 tacaaatatg ttttgcgtcg catcaaaagc cagcaaa
//
LOCUS       DAPCMU010000169         4477 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPCMU010000169 DAPCMU010000000
VERSION     DAPCMU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.174"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            323..1054
                     /locus_tag="RD744_002525"
     CDS             323..1054
                     /locus_tag="RD744_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDU0558492.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="RD744_002526"
     CDS             1199..3226
                     /locus_tag="RD744_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDU0558493.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="RD744_002527"
     CDS             3334..4431
                     /locus_tag="RD744_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDU0558494.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          925 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggttgg
//
LOCUS       DAPCMU010000170         4461 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPCMU010000170 DAPCMU010000000
VERSION     DAPCMU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.175"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            574..1008
                     /locus_tag="RD744_002528"
     CDS             574..1008
                     /locus_tag="RD744_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558495.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="RD744_002529"
     CDS             2298..3221
                     /locus_tag="RD744_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558496.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="RD744_002530"
     CDS             3237..3776
                     /locus_tag="RD744_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDU0558497.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAPCMU010000171         4437 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPCMU010000171 DAPCMU010000000
VERSION     DAPCMU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.176"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            531..1442
                     /locus_tag="RD744_002531"
     CDS             531..1442
                     /locus_tag="RD744_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDU0558498.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1929..2195
                     /locus_tag="RD744_002532"
     CDS             1929..2195
                     /locus_tag="RD744_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558499.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2267..2608
                     /locus_tag="RD744_002533"
     CDS             2267..2608
                     /locus_tag="RD744_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558500.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2663..3082
                     /gene="ssb"
                     /locus_tag="RD744_002534"
     CDS             2663..3082
                     /gene="ssb"
                     /locus_tag="RD744_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDU0558501.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3159..3455
                     /locus_tag="RD744_002535"
     CDS             3159..3455
                     /locus_tag="RD744_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558502.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3470..3901
                     /locus_tag="RD744_002536"
     CDS             3470..3901
                     /locus_tag="RD744_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDU0558503.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4083..4388
                     /locus_tag="RD744_002537"
     CDS             4083..4388
                     /locus_tag="RD744_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558504.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1629 a          698 c          951 g         1159 t
ORIGIN      
        1 gtcaaaaacc taagcgggaa gtctccgaat aacataccaa gcgccgacag ccaacagata
       61 aagaatacac tactaaaaat agtagtagca taaaatttaa aagggaaaaa atcaacccct
      121 accccagtct tggggtctca aaactaactc ccccctaacc ccctctttca gcctcttttg
      181 aaagaggggg agaatcaacc ccacggccaa cgccccagtg ttcttggggc tgtccagaac
      241 agaaacttgc gagagagtgg gggtaaaaca agtgtagagg aaacttgaag acttgttcga
      301 acagaaactt gcgagagagc cggagtaaaa caagtgtaga ggaaacttga agacttgttc
      361 gaacagaaac ttgcgagaga gttggagtaa aacaagtgta gaggaaactt gaagacttgt
      421 tcgaacacaa acttacaaga gaggggagta aagcagttgc agaggaatca ggaagatttg
      481 ttcgaacaga aacttgcaag aaagcattat taaattgaag gtgatgaaat atgaagtatt
      541 tattgcatgg ctattttatt gattattcta ctcgcaatag tagtacttta gcgattctat
      601 gtttgcttca tgaattgaga gtggttacta gaaaacagct atacaaacta ttgaattttg
      661 acagatcaat tagtaaacaa ggagtaacga atgctttagt gagattatat gaaaaacagt
      721 taatcgatcg aaaaaaatat ggaacagaaa tgtattatta tttgacgaaa gagggacatc
      781 aaagcatagg tgggtattat acgttgccga aagttcctga atataatttg aatcaccatt
      841 tacaaattaa caattatttg attaaaggaa tggcattggc aaaagaccac ccacatttaa
      901 aacggattat ttcagaaaga cgtaccgtct atgaaacgaa ggatttaaat cctaagacaa
      961 aaggaagaaa attttttgtg ccagatttta tttttcgttt tcgagacaaa gaaaatcatg
     1021 agatcaattg gtcctttgaa attgagctaa cgttgaaaac acgtcgtcgc taccgtgaag
     1081 cgatttttcc gaaatatatt cgtgagttgc gaacacgtcc tgaatcacat ttaatttatg
     1141 tgacacctag tggcgcaatt caagaagaac ttcatgcgtt taagcgtatt tttttcaagc
     1201 gcgaaactga aaaagatgcg agtcaacaag aattgtttga gcggttgcat attttttcag
     1261 ctttagagtt tgaagaggga ttaaaagcat tattagagaa tgatccagca gtcaattggc
     1321 ggacagtaga acgagaaaaa atttatccga atggcgctgt tcaaagtcgt ttgaatttat
     1381 ttgaagaatc aactgaaaat gaggaaaaac caaaagtgaa gtttacgcct gaagtattgt
     1441 aaaagagggt gttcgcttcg ctcttttttt gtgccagttt gctggcaccg ttcagacgca
     1501 agcgtctgaa ccggctcgtt tttaggaagg actaacttgt ttgatttatc cgttgtcaaa
     1561 gaagtggacg ttcgagagtc cctaatcgta tagcagtcat tcggcgagta taacctaaaa
     1621 cataacctgc caattgccaa gggtaaaatg cacgctgcgc gcccttggca atcgtcagaa
     1681 catgttttcg agccggttat gccgaacgac cgctatacga aaagggcctc taaacagaag
     1741 tccactttga tccttgacaa ctgaaaccca aaatcaaaca agcaggcctt cgtctaaaaa
     1801 cgagccgaat gaagaccttg ggggttgcac ccccaaaccc ccgtgccccc gacacactac
     1861 cgaaaaggtg ggggtgggtg gcgaaaaaat caaataaaac attctattca agaaaggaag
     1921 aaacaaccat gacaactaaa aacgagcaac tgaaagaaca ggcaagaaaa gagttgcaag
     1981 aaagaggttt gattattgag gggagctttg aaggagattt tgaaacctat attggttgtt
     2041 atgcaagacc gataaacaaa cctacagcct tagacccaat gaacgaacaa gaagtaaaag
     2101 aacaagaaaa acatgcagtg aatggttttc cgcaagattt cactgaatgg tatgagtggg
     2161 aaattaacaa cgggaagctt gaaaacttct tataaaagtc agatgacgtg caaagagaat
     2221 gattctcttt gtacggtaaa aatataaaga aagaaggaaa aagaaaatgg caaaaaacac
     2281 accttatcaa gtaggagaca ttttttatag tagttggggc tacgaacaaa cgaacgtgac
     2341 tttttggcaa attgtgaagt taacagaaaa aacagcatgg tttcagccta ttcaaaagaa
     2401 aacggtcgag cattattcgt ccatgagtgg aaaagttgtt ccaatgaaag acaagtttat
     2461 agagtatcct ttagcaaaaa ctaaagacag tattgtaaaa aaacgaattg atccagaaac
     2521 ctatcctgat atgttatgtg gttatcgtag ttgggacacg tttcatagat ataaaaatca
     2581 agctgtttat gaaagtagtt attattaagg ggatagtttc ccctttcaaa aataataaat
     2641 agaagaatgg agagaaaaaa tcatgatgaa ttcagtgaat ttagtaggac ggttaacaaa
     2701 agaagtggat ttaaaataca caccaaaagg caatgcgaca ggcacattta ttttagctgt
     2761 taatcgaaat tatacgaatg caaacggcga gcgtgaagca gattatatcc gctgtgtcat
     2821 ttggcggaaa gctgcggaaa ctttagcaaa atatactgga aaaggaagtt tgattggcat
     2881 taatggacgt cttcaaacga ggagctatca gaatcagcaa ggacagacca tgtatgttac
     2941 ggaagttcta gtgacagatt tctatttgtt agagagtaaa gaaatcaatg agcaacggtc
     3001 aaaatcaatg gcgaataatc aaaataattc ttcaattcca ggaaatgaga ttcaaatttc
     3061 agatagttat ttgccctttt aagtgaaaaa taattttgtt ctatacctga tttcttcagg
     3121 tatagaacaa aaaataaata gagatgaggc gattttttat gaaaatcatt gacagttacg
     3181 atcaacgaac catgcaatat ttgggctcgt ttgattatac agatgaaaat attattaatt
     3241 atgttgcaag tttacctcct tatcaaagcg ttcgcttggt agattttgca agtgatgaat
     3301 tggtattaac aacgatgggc aactttttag attatgtcgg agacaaaaaa tggttggaaa
     3361 aaattttgtc taaattgatt gcgaaacaaa caggagaaga acccataaat gcgattcaat
     3421 tgttcaatcg atatgaacaa gaaaacaatg attaaagagg agagaaaaaa tgaaaagatt
     3481 aaaacgaagt atttttattg tgctagcatt gattgatttt gttgggacag gttgtgaagc
     3541 aattgacgtt caaaaagctc ccttatcgga aataaaacaa gcaataacaa aacaagatga
     3601 agaacaaccg ctaaatacac aagtagaaat agtgaatcaa aataaaccga gtttttctga
     3661 agaagatttt tcgttagaaa atggaaccag gcaatttttt tcagatttgg atcgtttaaa
     3721 tcgggtaggt gtagcaagtg cacttttgca taaaagcagg atgccaacag aagaacgaga
     3781 ccgattatac attaagccaa ctggctggaa acaaaagaaa atgaagaatg gcgattatct
     3841 ttataatcgt tgtcatttga ttggctatca acgtgcgaca agtttcccca taagtatgta
     3901 atacgaaacg ggaggattaa tcttaagatt aatcacaact aacctatttg acagttggaa
     3961 tgaaagctat catgtctgct gaatcaactg gtctgatact cctacatgca ataaagtcta
     4021 ctaggaagtt attgtatgaa gctaggtaaa ggcggcagaa caatttctaa gtgtggaatc
     4081 atatgaagtt ggatatgccg ggacgctata aattactcag ggtgagactg tttttaatta
     4141 aactgacgaa cttgcgaatg tacgggtcta taaccgactt atattcgaaa gatataagat
     4201 tgcgaatgca aagaacatta tcgaaaagta agaatgttgg tcatgaaatt cgagatggaa
     4261 gactagcggt gtatctgtca agagttggta aaacatgttt gattgattca gatgagagtg
     4321 ggaatgcgac aaaacgtttt acagaagaaa tagagcaact agcagaactg aatatacgat
     4381 atttttaaat actttagcgg agaataaaaa aatcctctac agaaaactac ggagggc
//
LOCUS       DAPCMU010000172         4434 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPCMU010000172 DAPCMU010000000
VERSION     DAPCMU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.177"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(543..2048)
                     /locus_tag="RD744_002538"
     CDS             complement(543..2048)
                     /locus_tag="RD744_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0558505.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="RD744_002539"
     CDS             complement(2045..2731)
                     /locus_tag="RD744_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDU0558506.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="RD744_002540"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="RD744_002540"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDU0558507.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAPCMU010000173         4425 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPCMU010000173 DAPCMU010000000
VERSION     DAPCMU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.178"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            690..1658
                     /locus_tag="RD744_002541"
     CDS             690..1658
                     /locus_tag="RD744_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0558508.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="RD744_002542"
     CDS             1669..2484
                     /locus_tag="RD744_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDU0558509.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="RD744_002543"
     CDS             2552..3262
                     /locus_tag="RD744_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDU0558510.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="RD744_002544"
     CDS             3298..>4425
                     /locus_tag="RD744_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDU0558511.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAPCMU010000174         4402 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPCMU010000174 DAPCMU010000000
VERSION     DAPCMU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.179"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            801..1055
                     /locus_tag="RD744_002545"
                     /pseudo
     CDS             801..1055
                     /locus_tag="RD744_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1125..1637
                     /locus_tag="RD744_002546"
     CDS             1125..1637
                     /locus_tag="RD744_002546"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558512.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1634..2671
                     /locus_tag="RD744_002547"
     CDS             1634..2671
                     /locus_tag="RD744_002547"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDU0558513.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2831..3007
                     /locus_tag="RD744_002548"
                     /pseudo
     CDS             2831..3007
                     /locus_tag="RD744_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3062..4372
                     /locus_tag="RD744_002549"
                     /pseudo
     CDS             3062..4372
                     /locus_tag="RD744_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
BASE COUNT         1671 a          646 c          835 g         1250 t
ORIGIN      
        1 ttattgatga ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct
       61 atgtattctt cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc
      121 tttaggtaga tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa
      181 ataaatgcaa ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt
      241 ttttatgccg agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga
      301 gttggttgga cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag
      361 ccctgtaggt tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa
      421 tgagtgagaa aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc
      481 aatcattgat agtttattat acttaccagc aatataaatt atctaaagag aattaagttt
      541 tgattatgta ataagttttg gtactaaaaa agacttgatc taattagacc aagtcttttg
      601 ttagtgttat attaataaca aaataaaaat aagaagtcgc tcactccccg accaaagttt
      661 gtgagcaaaa taaacagtcg atgtttgttt cttaattgta tatcttgata ttaaacgatt
      721 taagattatt cttcaagata tatattcgca aatgtaattg gaaaagcgac ttcttaaaca
      781 aaattaagga gtcgcttttt atggcaaaaa taacgaaaaa tgagcaagaa aatttggtgg
      841 aaaattacac aaaaaaaccg gcgtactcta atagccggtt agacgcacat ttcgtgtgca
      901 cttctaatcc gaaattgagc tttgacgcta tgacgatagt cggaaacctt aataaaaaca
      961 gcgctaaaaa gctatccgat tttatgagtt tagatccaca aattagactt tgggatatac
     1021 ttcaaacaaa atttaaagct aaagcactac aagaccccct atgttaggct agttgtcgta
     1081 taggataata agttataata aagactatac ggaggatgat taccatgaca aataaaaaca
     1141 atgaacgtta ctctaccgaa ctgaaagcga cagccatcaa gaaaatgatg ccacccgaaa
     1201 atagggggat tcgccaatta agtcaaactt tgggcattcc agaagcgact ctttattctt
     1261 ggcgtaaaaa agctagaatt caagggaata ctacacccgg aacgaaaaaa aatgtcgaaa
     1321 aatggtcaag tgaagataaa tttttaattg tgatggaatc attctcaatg aaccaagtag
     1381 aacttgcgga atattgccga aagaagggct tatttaaaga acaaattgag gcttggaaga
     1441 aaacgtgctt atctgcgaat gaacaggaag aaaatcgaac gagagaactt gcaactgaac
     1501 taaaagaaga gaaaaaacaa gcgcgtcaat tagagaaaga attacgtcat aaggaaaaag
     1561 cattagcgga agccgctgct ctcttattac taagaaaaaa agcacacgcg atttgggggg
     1621 accaagagga agaatgattt catcttcaga tcgcaaacaa gctgttgaac ttattcaaga
     1681 agccaatcgc aatggggccc gtctcaccta tgcctgtaat gagctgaata ttagcgttcg
     1741 aacctacgaa cgatggacgc gtgaaggtac gatagcgcat gatcaacgcc ctttggcgaa
     1801 acgtcctgta cccaaaaata aactgacgga tcaagaacgc gaaaaaataa tagaaaccgt
     1861 ttcaaaaaaa gaatttatga atttgccacc tagccaaatt gtaccaaaac ttgcggattg
     1921 ctcgatttat attgcatcag agtctagctt ctatcgtgta ttgcgagaaa aaaatatgca
     1981 acatcaccgt ggccgaagtc aagtttcaca aaaacgcata ccgccaagtc accttgcgac
     2041 gaagccaaat gaagtttgga cttgggatat tacttggcta aaaggtccaa taaaaggact
     2101 cttttaccgg ttatatctca tcattgattt atttagccgt aaaatggtag gctgggagat
     2161 ttgggaatca gaagaagctc gtcatgccga aaaacttatt aaacagacgg tgcttaagga
     2221 acgtatccaa ggacagccgt tggttttaca ctccgataat ggcagcccta tgaaagccgc
     2281 gacttttcaa gtcttactcg aaaaattagg aattcaaagt tcttattcaa gaccacgagt
     2341 gagtaatgac aatccttatt cggaagcggc tttccgaacg ttaaaatatc gaccagaata
     2401 tcccaataac ggctttaaaa caatagatga tgctcgaaaa tggacgaaag agtttgtcga
     2461 ttggtataac gattctcatt tgcatagtgg gattcaattt gtgtctcctg cagcgtgtca
     2521 tgaaggacga catctcgcta ttttagagaa acgaaagcag gtttacgagg acgctaaaaa
     2581 gcgccatcct gaacgctggt caaaaggtgt tcgaaattgg actccacatc aatcggtggc
     2641 gttgaatcct ttaaaagaaa cagaaagtta acaggagcat aatctttcaa gattatcaaa
     2701 taaccctcgt cacccaatag gggaaaaagt tattcttccc ctattggata acgaggccaa
     2761 ccgaccatag ggaggttctg ctttttgata ggtggaaaat aaaaatacga caactatatt
     2821 gacaaacacc gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
     2881 tgcctctaca acgattaaag gcatggaagc catccgagga ttatataaga aaacccgaaa
     2941 agaaggcact ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc
     3001 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
     3061 cgaatcatta aaaaaacatt atacagagcc ttgggaagat gaagttttta gagtaattat
     3121 tgaatataac aatgttccta ttggatatgg acaaatatat aaaatgtatg atgagttata
     3181 tactgattat cattatccaa aaactgatga gatagtctat ggtatggatc aatttatagg
     3241 agagccaaat tattggagta aaggaattgg tacaagatat attaaattga tttttgaatt
     3301 tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac cctcataaaa ataatccaag
     3361 agcaataagg gcataccaaa aatctggttt tagaattatt gaagatttgc cagaacatga
     3421 attacacgag ggcaaaaaag aagattgtta tttaatggaa tatagatatg atgataatgc
     3481 cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt tcaaagtaga
     3541 tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta ataatgaata
     3601 catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat
     3661 atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc
     3721 gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac
     3781 accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc
     3841 cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga
     3901 taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt
     3961 aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt
     4021 ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga
     4081 tggcaataat agattaactg gaataattga ttttggagat tctggaatta tagatgaata
     4141 ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga
     4201 agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat
     4261 agttgaagaa tattatccta ttgaaactat tgtttatgga attaaaaata ttaaacagga
     4321 atttatcgaa aatggtagaa aagaaattta taaaaggact tataaagatt gattatataa
     4381 tatatgaaaa gctattataa aa
//
LOCUS       DAPCMU010000175         4376 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPCMU010000175 DAPCMU010000000
VERSION     DAPCMU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.180"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..338)
                     /locus_tag="RD744_002550"
     CDS             complement(<1..338)
                     /locus_tag="RD744_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558514.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="RD744_002551"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="RD744_002551"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDU0558515.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="RD744_002552"
     CDS             complement(1495..2922)
                     /locus_tag="RD744_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0558516.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="RD744_002553"
     CDS             complement(2953..4167)
                     /locus_tag="RD744_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDU0558517.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1374 a          928 c          775 g         1299 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4321 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct tttttt
//
LOCUS       DAPCMU010000176         4375 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPCMU010000176 DAPCMU010000000
VERSION     DAPCMU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.181"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(77..1051)
                     /locus_tag="RD744_002554"
     CDS             complement(77..1051)
                     /locus_tag="RD744_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0558518.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2906)
                     /locus_tag="RD744_002555"
     CDS             complement(1500..2906)
                     /locus_tag="RD744_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDU0558519.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGFSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3127..3489)
                     /locus_tag="RD744_002556"
     CDS             complement(3127..3489)
                     /locus_tag="RD744_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558520.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3493..3831)
                     /locus_tag="RD744_002557"
     CDS             complement(3493..3831)
                     /locus_tag="RD744_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558521.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3810..4229)
                     /locus_tag="RD744_002558"
     CDS             complement(3810..4229)
                     /locus_tag="RD744_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558522.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4222..4362)
                     /locus_tag="RD744_002559"
     CDS             complement(4222..4362)
                     /locus_tag="RD744_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558523.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1236 a          975 c          626 g         1538 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attccgcaaa ggtttctcgc aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca acaaatccct gtattttttc tttgtattct
      241 tagctagatt tccccactca gaatgatcat aaagcaaacc tttcaccata atccgttcag
      301 taactttagt atttggcgga aaataatgtg gactagcaaa gctatccaaa ctttcatgaa
      361 ccatctcatg ccaatcagtt gcttttcccc ttcgtaactg caattctagt cgccaccatg
      421 tcgttatttc cggcggaaca attttacgtt ttctttcttg ttccagtttt ttattataca
      481 tccgaatttg tcgttcactg gcacgactac cccaatatgc cgtttctaac ttaccagaac
      541 gcccataaat tggtttaaaa gacacaggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ccgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccattacctt
      841 catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga gtgtgataat
      901 aaatattttc tttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttgtttg ctaactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaggtt
     1381 ggccaaaaaa tggactggcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc acatcgtaac
     1501 taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt ttgacgataa
     1561 aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat atccttttct
     1621 gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt ttttaatcgc
     1681 tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc cacttgtaaa
     1741 tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc tacatactca
     1801 tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt atcagcattt
     1861 ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc attcccagat
     1921 aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat atctttttgg
     1981 aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc ttcaatcttc
     2041 actctctcag aagccaccag agccttttct cgctcatttt cttctctttg cctagaaata
     2101 tacctcaact catcagaaac gctctcacgc tcttttacgg ccgtttgacg caattctgaa
     2161 atctcgctca tgatttcttt atattcgtga acatctttga ttttgttagt accgactaac
     2221 tcacgctccc aaccaaaatc tgccatcaaa cgttcaattg actcaacttc ctgattccta
     2281 aaattattcc aaatcactcg accattctct ttatctgctc gtaaaccctg ctgttgcaag
     2341 gctttgttaa aacttggttg cttttgaaca ccccgtttat acccttcagc aacaggaaca
     2401 atattcaaat gcaggtgcgg acttacttcg tcattatgaa taacagcatt gtagacggat
     2461 aaattaggat tccgttcttt aaattctttg aaatattcaa ctaaaatttt gttggcaatc
     2521 actcgatttt gttctgcttg ttgttccgat atgccagtag aaaaaccatc atcacgagta
     2581 gtacgaaaat catcaagagt accaatttgc acgataaatt ctcgctgtgg ttccaatttt
     2641 ttatccttgc gaacctttgc taaatagtcc tcgatttttc tatcggctct tttctgcttt
     2701 gcattatatt tttctacggc ttcaccgaat aattcttcat atttttcacg aatatttgtt
     2761 tgttctaaat agatattttc atccgatttt gtgaaatcaa tatgcttatg ccagtcattg
     2821 attttttggc tatcagttaa ctcacgatta ttatgagaaa tgcttgtatt ctttgttccc
     2881 tttttgaacg aaatactgaa ccccaagtta aaaccctcct tcggctgtcg ctaataaaaa
     2941 gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg
     3001 ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag
     3061 gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat
     3121 aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct cactcatctg
     3181 acttttctta ttcggtatca ctccgaataa gccgtcagat tcatcgggat aatctcgttc
     3241 gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt cgtgttgctg
     3301 atcgtcagaa atcgacgata gccaccgctc aaaatcagca aaagtttcaa acccaaaacg
     3361 cttccgaaga gccttgccca ttgcagcatt ttctttctca atcaagcgtt ttttctgttg
     3421 gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat ctgctaaagt
     3481 cattcccata aactacatct ccttttcttt ggaattaata acggaaattt ggtttaactg
     3541 cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc
     3601 taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctaaat cagcattgat
     3661 aaccgagttg agaacatcca tcattcgttc aagcatttca ctataattat cttcatccgg
     3721 caaattcaaa taatcagcta accgttcata catcaattca attctatcga ctgtttcttc
     3781 tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca ttttcgattt
     3841 tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag cagggtcaat
     3901 ttttgttgat cacgaatcat ttctgttaat tcagaaatat gtttgtcctt ctcagctaat
     3961 tgatcacgca aaaactcatt ctctttttct aaaggaaaat cactgcctgt gttattgacc
     4021 ggtaattgac cagtcaattg atcacttttt gaccgataaa ttgaccgaac aacaccaatc
     4081 aactctttat ctagcatgat aatgccattt tctttatact gagcaaggtc aaaccctgaa
     4141 ttgaccagtt gattgcgcac tgtatttttc gatacaccca attcgtcagc aagatttttt
     4201 atggtcatct tttctgacaa atcagtcatt agcttctcca ctactttcag ctaccgcata
     4261 aatatattta acggcgtgct tagggataat aatcggagag tcaattttat tatttttcaa
     4321 aatcaattca tattgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DAPCMU010000177         4371 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPCMU010000177 DAPCMU010000000
VERSION     DAPCMU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.182"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            55..1356
                     /locus_tag="RD744_002560"
     CDS             55..1356
                     /locus_tag="RD744_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDU0558524.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1383..1643
                     /locus_tag="RD744_002561"
     CDS             1383..1643
                     /locus_tag="RD744_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDU0558525.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2642..3877)
                     /locus_tag="RD744_002562"
     CDS             complement(2642..3877)
                     /locus_tag="RD744_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDU0558526.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1278 a          695 c          894 g         1504 t
ORIGIN      
        1 tttgcgttgt catgcaatga atggttcata ttttattgaa aaggagtggt tttagtggaa
       61 aagctgatga aatgggtcga agagaaactt gtcccaccaa tggcaaaaat tggtacacag
      121 cgtcatttat tagcaattcg taatggggtt gtttcaacat tgtcattgat tttgattggg
      181 acatttttta tggtctttat caatttaccg tttcctggat ggaatgaatt tattgcccct
      241 tattcggcaa cgattgtttt accctttagg atgacgatgg gattgatggc tatttatgca
      301 acttttgtta tgggttcgga tctagctaaa tcatatggat tagatagtgt gacaggtggt
      361 atattatctt taggaacgtt ctttatgtta caagttcctg taaacgtttt aactcctgaa
      421 gaggcacctc ttggctgggt cttacctatg agttctttgg gagcatctgg gatgtttgcg
      481 gggatcttat ctatgatttt tgcagtggaa gtatatcgtt tctttaaaaa tcgaaatatt
      541 actatcaaaa tgccagaaca agtgccacct gctgttgccc gtagttttga ggcattgatt
      601 ccaggtgcag tcattttaac aattacttgg ctgattcgaa gcgtcgcggg ctttgatgta
      661 aacgctgcct tattatcgtt attcgagcct ttaaccaata ttttagggaa taatttgctt
      721 ggtgttttac taccaatgtt tttgatccat ttgttgtggt catttggtat ccatggcatg
      781 agtattgtag gggctgttgt tcgtccaatg tggctgatca tgttggatga gaatgcaaag
      841 gcattggcag atggtacagc tgccacccaa ttaccctata tcgctcctga acagttttat
      901 caatggacgg taacaattgg tggtgcagga gcaacgatcg tggtatctgt tttgtttata
      961 ttcttctgta aatcaaaatt cttgaaagag gttggtcgtt tttctattat tcctggtatc
     1021 ttcaatatca atgagcccat aatttttgga gctcctatga tgttgaatcc ttacatgttc
     1081 atcccgttca acttagttcc tttagttttg actattatca gttattttgc agtcaaatta
     1141 gaaatggtca atagatttac tgttttgcca acatggacat taccagctcc aatcggcggt
     1201 tatctttcag caggaaatga ctggcgtgtt gttatcttga tcatgatcaa cacattgata
     1261 gcatttatta tctattatcc atttgtaaaa gcttatgata aaaagatgct gtcagatgaa
     1321 caaagtcgtg gagaaaacga agtccagact gtctaagttt ggtttttaga aggtgggaaa
     1381 caatgtattg gctgatttta ttttttgttt ttatcttttt attaacggct agtcacctta
     1441 tattgaatat gctagctaca tatcatattc aaatcaatcg atggatttgg gcactggcat
     1501 catttttgat cgtgattctt ccaaagatca ttgtaccgca tatgaatgtt ttgttttcat
     1561 gggggacata tgtactttgt ggaatttttg ctatcaattt tatgatcgag cagcatagat
     1621 ggtttgtcac atcgaagctt taaatgttct aaaagtggtt gtaacagaag tggttagctt
     1681 caagaaataa gaagaaattt acgaaaattg cttcttaaat ttttgtgaaa tgttggctta
     1741 tttttcgttt ggttgaactt attaccgcag atgcaagctt ttgaccaccg tttattcgga
     1801 atatgggact gtgacaaggc ttttgtcaca gccctttttt acataaagat tattattgtt
     1861 ttaactgttt tggcagaaag aaggaggaaa aaatggaaat tttatcagca atggatttag
     1921 aattagtaga tctactacca aagtgatcaa gtcaaatata gatttgggag cttacaacca
     1981 tatagtaggg gctgcagtat ggtgtatgaa tggtaatatg tatgatgtga gtgtttattc
     2041 aaatcatgaa acgtgtatag aaataattgt gctgggaata gcaatcagtc atggtgaacg
     2101 tgacttttag tgtatcgttg ctgacgacat agaacattct gacatgatct actcaatatg
     2161 tggggattgc tggcagttta tataggagta tgcatcggat tgcgagatca ttgtctcaac
     2221 agaagaggga ggttgattat caaagataaa gtggtaaatc ctacatttga tttagtaatt
     2281 gttgagggga ctagcggcac aaatctctac aaagcgatgc ttctagatct atatgacaca
     2341 ttttaagaaa atgtatttgc ctaattatta tggtctttct tttgaagaaa cgttgagatg
     2401 atccaaccag agaaaggtgt caaagaattt gactaagaat aaatggcaga cgctacctat
     2461 gctcatttgg atagcgtcag atcttttatt ttaaggatga ttttgtgttt tttagacacc
     2521 gtaatttaag taactctaac ttttcaatcc actatttact ttttttgtat tttcactgtc
     2581 tgggtctcga atagcgaaat tactgatgag tctctattaa aaaagctttt tctacttgaa
     2641 actatattgg gtggttgctc aggaaggttt cgatttcttg aattgcttgt tgtggaccat
     2701 ttattggaaa aacggcttgc agcgtgcgtg cctgctgacg aatcttgggt gaagaaactt
     2761 cgcgcagagc aatctgcagt tttctttcag tcactggttt tgtgagggcg attgcgtttc
     2821 cgtattttac acaatagtca atattacagc tttgctcaaa ttgcatcccc acaccgataa
     2881 acggtttgcc ggattcacag gcagtttgga cggtcccttc cccgccgtgg ataaaagaaa
     2941 aatcaatgaa ttcatgcaaa agatgactag acagatattc tgttattaaa agatcttctg
     3001 agtcagattg aatgtcatca agcagatagt caataggtgc aataaccgct agatccggtt
     3061 gtttttgtaa gaagaacaaa attttgcgta acatacgcgg attgcctgaa ctacccattg
     3121 cgaaatagat aatacgtttt ctttcttgtt gctttcgctg tataaaagtt agcacttctt
     3181 caggcaagga agtactgagc ttagcaaaaa ttggaccaac gattcgataa ttttccggca
     3241 gctgttcttc ttccaaaaat agtttaggcg ttgtgatgag atttagatca gcatccatca
     3301 tttgtaaact tgtttttggt aaaggaagca tttctttttg tgcagcgtgt ttgaaggcat
     3361 tggggatcca tcgggtattt actatacgct tttttgtcca gtgccaaagg ggattgctta
     3421 aagggccgaa gaaacgtaac tcgtggggca tcagcgcatg ctctgattga aaaaaaggtt
     3481 cactcaaagc atagggttta acatatacta aaggaatctt tttattgcgg gccgataaaa
     3541 aaattgtcac attcgaacct gtcacaatca gtcgaggctc aaacttagca atcagactct
     3601 gttcactagt gatacgagag gcaaccattt ctttggtaaa agggttttta agtgtacgca
     3661 actggtcaaa ttttatgatt tgttcttctt gttttttcgt aaggatcggc gctagtaaat
     3721 gatactcaaa gccactttct gtgatcaaag gagcaaaagt tttagaaaag ccaaagaata
     3781 cacagtaata acggtctttc atagctttgg caacctcaat cattcgtgtt gtctcagcta
     3841 aattgaatac tgcaggagca aaaagaattg tcttcatgaa atcctcctta ttggaatgag
     3901 tctatccctt cgtttcttac tagtatacta aaaaacacag cgttcgagta gcctatgtat
     3961 attttgaata ttaaactgtc tctcgctgtt ttcacaactt ctaaatttaa attttgtaat
     4021 caagttgaaa ctccaatttt caaaaaaaca cgcaacaatt ttttgatcga tttatatgtt
     4081 gtgccagata aatacgagaa gttgtgtcat taaaaataaa catgagaaca taaaaaaata
     4141 aaataattat attaattgat aggtgtgctg tcgtcattat acacacgtaa gaaagaaata
     4201 gttatgttta aaaatattct tagactttct cgccatgaca attttcacta tgtgttataa
     4261 ctgtaaacga taattattca gataacacaa aaagaaatca aggagcagat acgccagttg
     4321 tttaatttga atattatttt aaaattaaaa tttttattct gttctaaaca t
//
LOCUS       DAPCMU010000178         4348 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPCMU010000178 DAPCMU010000000
VERSION     DAPCMU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.183"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(387..1853)
                     /locus_tag="RD744_002563"
     CDS             complement(387..1853)
                     /locus_tag="RD744_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDU0558527.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="RD744_002564"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="RD744_002564"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDU0558528.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAPCMU010000179         4238 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPCMU010000179 DAPCMU010000000
VERSION     DAPCMU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.184"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(152..427)
                     /locus_tag="RD744_002565"
     CDS             complement(152..427)
                     /locus_tag="RD744_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDU0558529.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="RD744_002566"
     CDS             complement(399..1352)
                     /locus_tag="RD744_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDU0558530.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="RD744_002567"
     CDS             1964..3457
                     /locus_tag="RD744_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDU0558531.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..3888
                     /locus_tag="RD744_002568"
     CDS             3592..3888
                     /locus_tag="RD744_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDU0558532.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3998..>4238
                     /locus_tag="RD744_002569"
     CDS             3998..>4238
                     /locus_tag="RD744_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558533.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKVNKRRCGRTEIIFPAEEKEYI"
BASE COUNT         1427 a          698 c          764 g         1349 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggttattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc cttagcttct
     3781 ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga gtatattgag
     3841 tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta gtttttattt
     3901 acgccgtttg tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatta
     3961 taatacccgc taaagcatac aaggagagtg aacacaaatg acttataccc atcttacacc
     4021 aaacgagctt gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc
     4081 gaagcagctt aaacgtggac gccaaacaat ttacaatgtc tataactttc tcaaatgtgg
     4141 tggaacagca cttgaatact ttgaacaata caaagtaaat aagcgacgtt gtgggagaac
     4201 cgaaatcatt tttcctgctg aggaaaaaga atacattg
//
LOCUS       DAPCMU010000180         4234 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPCMU010000180 DAPCMU010000000
VERSION     DAPCMU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.185"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(48..1814)
                     /locus_tag="RD744_002570"
     CDS             complement(48..1814)
                     /locus_tag="RD744_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558534.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1774..3528)
                     /locus_tag="RD744_002571"
     CDS             complement(1774..3528)
                     /locus_tag="RD744_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558535.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3638..4207)
                     /locus_tag="RD744_002572"
     CDS             complement(3638..4207)
                     /locus_tag="RD744_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDU0558536.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1427 a          806 c          592 g         1409 t
ORIGIN      
        1 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
       61 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
      121 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
      181 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
      241 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
      301 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
      361 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
      421 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
      481 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
      541 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
      601 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
      661 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
      721 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
      781 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
      841 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
      901 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
      961 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     1021 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     1081 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     1141 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     1201 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     1261 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     1321 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     1381 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     1441 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     1501 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     1561 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
     1621 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
     1681 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
     1741 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
     1801 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
     1861 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
     1921 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
     1981 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
     2041 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
     2101 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
     2161 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
     2221 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
     2281 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
     2341 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
     2401 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
     2461 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
     2521 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
     2581 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
     2641 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
     2701 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
     2761 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
     2821 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
     2881 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
     2941 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
     3001 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
     3061 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
     3121 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
     3181 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
     3241 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
     3301 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
     3361 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
     3421 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
     3481 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
     3541 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
     3601 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
     3661 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
     3721 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
     3781 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
     3841 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
     3901 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
     3961 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
     4021 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
     4081 caaaaatccc gggggatatg ctgtgaccaa acgccccacc aatactacta accattattg
     4141 aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata taatcttttg
     4201 ttttcatata tattctcctc aattctattt cgtt
//
LOCUS       DAPCMU010000181         4210 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPCMU010000181 DAPCMU010000000
VERSION     DAPCMU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.186"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1481
                     /locus_tag="RD744_002573"
     CDS             <1..1481
                     /locus_tag="RD744_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDU0558537.1"
                     /translation="SDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRVE
                     SLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFVF
                     LLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAIIKLFNTILTRAGITLIIT
                     TAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLSDLMSMFSLQSNDSQSVGSRVM
                     RKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSSGRTQADHTRPDGQE
                     KSTLGKRIGQTIGAVADTKDKIVDSAGNLKEQVKDIPTNARYAVYQGKSKAKENVRDL
                     TSSISQTRADRASGRKEQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQEQSH
                     DGQTAKQSSIQTSRMESQQAKQERPAVKSDSLSLKAERQNRTTQERTVQKPATSTTPA
                     DRASQRPVTKERPSTVQRVQSQNLRNRPPIKSATIKKGSKKP"
     gene            1478..2479
                     /locus_tag="RD744_002574"
     CDS             1478..2479
                     /locus_tag="RD744_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDU0558538.1"
                     /translation="MKLKALVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            2476..3408
                     /locus_tag="RD744_002575"
     CDS             2476..3408
                     /locus_tag="RD744_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDU0558539.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            3653..3769
                     /locus_tag="RD744_002576"
     CDS             3653..3769
                     /locus_tag="RD744_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDU0558540.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3785..>4210
                     /locus_tag="RD744_002577"
     CDS             3785..>4210
                     /locus_tag="RD744_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDU0558541.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQ"
BASE COUNT         1387 a          800 c          869 g         1154 t
ORIGIN      
        1 attccgatag tcaaggcaag gacagcgtgg acttaatcag agatagcctg ttttccatac
       61 aggttcagca accgtggcta ctgcttcaat acaacagttc agacattgaa agtattggtg
      121 ctgaccgtgt ggaaagtcta ctttcaacca gtccagacag taataatgga gaagacagag
      181 aaaaaattgt agcagaagaa atcgaagaca gaaacaatgt gaacatgacg attacaaaaa
      241 ctattaaccg tttgggtaca gtcttctttc tctttgtatt caatatcggg atttctatat
      301 tcgtattcct actaacagga atcatgattt tctcgcaggt actttttatc atctatgcca
      361 tgtttctgcc tgtgagcttt attttaagca tgattccgtc ctttgatggt atgtcaaaac
      421 gagccattat caaactcttt aataccattt tgacacgagc tggaatcacg ctgattatta
      481 cgacagcctt tagtatttca accatgctct ataccttatc ggctggttat ccgttctttt
      541 tgattgcctt tttacagatt gtgacctttg caggaatcta cttcaaactg agcgatttaa
      601 tgagtatgtt ttcgcttcaa agcaacgatt ctcaaagtgt gggaagtcgt gtgatgagaa
      661 aacctcgtat gcttatgcac gctcatatgc accgcctaca gcgtaaactt ggacgctcca
      721 tgacagcttt aggtgctggc tctgccattg ccagtgctac aggaaaacaa ggacagtcgg
      781 gtagttctgg taggacacaa gcagatcaca cccgtccaga cggacaggaa aaatcaacac
      841 ttggaaaacg tatcggacaa acaatcggag cagttgctga taccaaagat aaaatcgttg
      901 attctgctgg taatctcaaa gaacaggtta aggatattcc gaccaatgca agatatgcag
      961 tgtatcaagg aaaatccaaa gcaaaagaaa atgttcgtga cctaacttcc agtatttctc
     1021 aaaccagagc agacagagcc agcggacgca aggaacagca ggaacaaaga cgcaaaacca
     1081 ttgcagagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca gcttcttccg
     1141 ttcatgaaag accagctact aagcaagaac aatctcatga tggacagacc gcaaaacaat
     1201 cttctattca gacttcacgc atggaatctc aacaagccaa acaggagcgt ccagcagtta
     1261 agtccgattc tttaagttta aaagcggaac gacaaaaccg tactacacaa gaaagaacag
     1321 ttcaaaagcc agcaacttca actacaccag cagacagagc ttcacaacgt ccagtcacaa
     1381 aagagcgtcc gtctactgtt caaagagttc aaagccaaaa ccttagaaat agaccaccaa
     1441 taaaatccgc cactatcaag aaaggcagta agaaaccatg aaactgaaag ctttagtgat
     1501 tggtggttct gggctattct tgatggtctt ctcactgctt ctgtttgttg ccattttatt
     1561 ttcagatgaa caggacagcg gaatttccaa tattcattat ggaggtgtga atgtttccgc
     1621 agaagtgctg gctcataagc ctatggtaga aaaatatgcc aaagaatatg gcgttgaaga
     1681 atatgtcaac atacttcttg cgattataca ggtggaatcg ggcggtactg cggaagatgt
     1741 tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat
     1801 taagcaaggt gtgaagtatt tcagtgaatt attagccagt agcgaaaggc tcagtgtaga
     1861 tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttagggt atgtggctaa
     1921 tcgtggaaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg
     1981 cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa
     2041 ctatggcaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa cagagtttga
     2101 tgatgatacg gtacaagcca tcatggacga agcactgaaa tatgagggct ggcgatacgt
     2161 ttacggtgga gcttccccga ctacttcttt tgattgtagc ggactgacac aatggacgta
     2221 tggaaaagct ggaattaact taccacgaac cgcacaacag caatatgatg tgacccagca
     2281 tatcccacta tcggaagcac aagctggcga tttggttttc tttcattcta cctataacgc
     2341 tggctcttat attactcatg ttgggatata ccttggcaat aaccgtatgt ttcatgcagg
     2401 cgacccaatc ggttatgccg acttaacaag cccctactgg caacagcatt tagtgggagc
     2461 aggacgaatc aaacaatgag aaaggaagat ttaatgatga aatttagaaa aaatcagaat
     2521 aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa
     2581 aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag
     2641 cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac
     2701 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
     2761 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
     2821 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
     2881 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
     2941 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
     3001 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
     3061 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
     3121 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
     3181 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
     3241 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
     3301 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
     3361 gatttggtac ttgaaaagaa cgggagtaat tggaagattg taaaataaca aatattggta
     3421 catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg agattccttt
     3481 acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt aataaatatg
     3541 cggcaaggta ttcttaaata aactgtcaat ttgatagcag gaacaaataa ttagatgtcc
     3601 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
     3661 aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag
     3721 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
     3781 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
     3841 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
     3901 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
     3961 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
     4021 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
     4081 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
     4141 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
     4201 ggtttatcag
//
LOCUS       DAPCMU010000182         4197 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPCMU010000182 DAPCMU010000000
VERSION     DAPCMU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.187"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(587..952)
                     /locus_tag="RD744_002578"
     CDS             complement(587..952)
                     /locus_tag="RD744_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558542.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1028..1273)
                     /locus_tag="RD744_002579"
     CDS             complement(1028..1273)
                     /locus_tag="RD744_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558543.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1343..1597)
                     /locus_tag="RD744_002580"
     CDS             complement(1343..1597)
                     /locus_tag="RD744_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558544.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1761..2084)
                     /locus_tag="RD744_002581"
     CDS             complement(1761..2084)
                     /locus_tag="RD744_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558545.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2271..2344
                     /locus_tag="RD744_002582"
     tRNA            2271..2344
                     /locus_tag="RD744_002582"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2305..2307,aa:Ile,seq:cat)
     gene            complement(2729..3226)
                     /locus_tag="RD744_002583"
     CDS             complement(2729..3226)
                     /locus_tag="RD744_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDU0558546.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3266..4075)
                     /locus_tag="RD744_002584"
     CDS             complement(3266..4075)
                     /locus_tag="RD744_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDU0558547.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1412 a          733 c          731 g         1321 t
ORIGIN      
        1 ttttaaagaa tatttagaaa ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat
       61 tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt tactatcaat
      121 attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac tgatcacaaa
      181 atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg actacagagg
      241 cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc gatgtagtaa
      301 tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct
      361 gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg
      421 ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt ttagttttgc
      481 acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta agatggttaa
      541 agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag gtaagataaa
      601 ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg tgttccggat
      661 aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat gatttgccac
      721 gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc atttcatcca
      781 tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca taatccacag
      841 tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga tgttgtccta
      901 aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc atagaaatgt
      961 ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc accaaggctg
     1021 gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa
     1081 tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct tctccttctt
     1141 cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct acttgagata
     1201 aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt aaaatagctt
     1261 cttcgatact catttaatca cctttcatta tagtagaata gttataatga ctatagaaag
     1321 ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa taaagaatga
     1381 cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta tcgggttcaa
     1441 agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc aaaatagcta
     1501 aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc agtgcgaccg
     1561 caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct caacgattca
     1621 tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg aaaataaaag
     1681 aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa gcaggaactg
     1741 ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc taagtaattc
     1801 aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat gatcccatag
     1861 cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct ttttccttta
     1921 tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat cattccaata
     1981 aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc cgcaactaaa
     2041 tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa taaataaaag
     2101 aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag ttatgacaca
     2161 agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt gtgagagttt
     2221 gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat gtacctttag
     2281 ctcagttggt tagagcagac ggctcataac cgtccggtcg taggttcgag tcctacaagg
     2341 tacataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa
     2401 cagcttctta tgtttttact tggctgttct tattactgca gatgcaagct tttgaatacc
     2461 gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag tattagctta acaaacggaa
     2521 aaatagttcc tcatattttg ccgtttgtta agctaatacc gagggtcagc tgattgaaca
     2581 ccatttaaga aagaggtcgt gagcctgccg tttcactctg tgtcataaaa aagccaatct
     2641 actttgatag aaaaagcacg taggactaaa acagttctgc gtgctttttt tattgtgtgt
     2701 taaatatcgt aaatgaagat agatattttt atgattcgac gatagagacg ttgaaatctt
     2761 tcaacagctc taaagcggat tctccgtgtt ttctactgga gactaacatt ggatcaacaa
     2821 aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag attagagagt acacacaagt
     2881 ttgtttcaat cccgactact tcgatctctt tttgttcttc tttgctttca aataaagttt
     2941 cctggatttc agctaaagtt tcaggtggaa tcgtaaagta atacttttta atcactcgtt
     3001 cattttcttg tggagaaaga agagggatca attgcaaatc ttctcgttct tcttctttgt
     3061 ccttcagttc aactggtata tcccgtgtga agagaatata ttcattattt tggcgtgctt
     3121 tagctaatct ttgagcaata cgttctgcca attgatctga ctctggaata taaaattcgc
     3181 tgtttggatc aagaatatga ttttgcatat caataataac taacattttc tttcctccta
     3241 cattcatatc gtcattttat tctttttaat agaagttttt taaaatataa gcaacaccag
     3301 cttcttcatt cgtcagtgtt acttggtttg caatttcttt tacttgtcgc tgtgcattcc
     3361 ccatcgcaat actcaacccg ctgtaattca acatagcaat gtcgttttct tgatcgccaa
     3421 aagccatgat gtcactcgat tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag
     3481 ctttagtgat acctgtaggc gtgatttcta agaaatgcgg atcagagaat actgattgga
     3541 taccatctgc ttgcagatct ctttgtagct tctgtagttt ttcttcactc ttatctgtta
     3601 atgctaattt aagatagcta agagaaggat cttccaacat atttaaaatt tgatcaaaag
     3661 aatggttctg taaattgaaa tgttcataat aagggtcatc tttgactaga tttgattcat
     3721 cgaatgtcag ctgatcatct tgggtttctc ggctagcaat gatggatatt ttcgcttctt
     3781 gagcatagaa gaaggctttt ctcagtgtat cgctatctat ttttgaacgt ctgagaactt
     3841 tcgccatttt tccatccttc atttcagcaa tcagtgaacc attggaagaa atcaaatagc
     3901 caccgattcc taattcggaa aaaatatctt tagcagaaat gctgttcctt ccggtagcga
     3961 cggttacgat tccgccattt tcatcaaatt tccgaagtgc ttggatattt tcttctggta
     4021 tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat cgctgctagt ttcattttat
     4081 cttcctactt tctttttaca tatctatttt cctgttacct taagattaaa tgagaattta
     4141 aaaaaatgaa actaacttgc tttgtcatta tgaatttttt caaaaaacat tcttaag
//
LOCUS       DAPCMU010000183         4175 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPCMU010000183 DAPCMU010000000
VERSION     DAPCMU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.188"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..208)
                     /locus_tag="RD744_002585"
     CDS             complement(<1..208)
                     /locus_tag="RD744_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDU0558548.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYL"
     gene            complement(205..723)
                     /gene="msrA"
                     /locus_tag="RD744_002586"
     CDS             complement(205..723)
                     /gene="msrA"
                     /locus_tag="RD744_002586"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDU0558549.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(743..1456)
                     /locus_tag="RD744_002587"
     CDS             complement(743..1456)
                     /locus_tag="RD744_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDU0558550.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1459..2355)
                     /locus_tag="RD744_002588"
     CDS             complement(1459..2355)
                     /locus_tag="RD744_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDU0558551.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2513..3355)
                     /locus_tag="RD744_002589"
     CDS             complement(2513..3355)
                     /locus_tag="RD744_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDU0558552.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3487..4140
                     /locus_tag="RD744_002590"
     CDS             3487..4140
                     /locus_tag="RD744_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDU0558553.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1081 a          793 c          757 g         1544 t
ORIGIN      
        1 ctaaatattt ttcccatgct tcgtcaaaga tatccatgga cggcaaatag tcgacattca
       61 gttccagata attagaaatt tcttcgtagc tagagctgtg cttaggaaat tggatgtctt
      121 taccggcttc tgtagcgaaa acagataaag catcatgtgt gccaccgcct cgcagtgtca
      181 ttaaataatg aaaaaagctt cgattcaccc ttcgttcctc caattttctt cgataaaggc
      241 agcccggcgt gcatggtttc ttttaaaatt ttcaggatgc tttttataat actcttgatg
      301 gtcttcttct gctagataaa atggttgggc tggttcgatt tttgtaacga ttggctgttc
      361 gaaacgtcca ctttcagcta actgtttctt acttttttca gcaatgatcc gctgttcttc
      421 tgtcaaataa aagatcaccg ggcgataatt gtcaccgcga tcctcgaatt gtccgaaagc
      481 gtcagttgga tcagtttgct gccagtaaag ttctactaat tgttcatatg aaatcaatgt
      541 tggatcatag ataatctcca ctgcttctgt atgtccagtc gtttggcttt ttacttgttc
      601 ataagtcgga tctggtacat gccctccagt atatcctgac atcactgtat ggataccagg
      661 aagcgtgtcg aaaggctgga tcatacacca gaagcagcct cctgcaaata ttgctcgttc
      721 cattatgatt ccccctctaa tactactcat ccgatgattt tttattttct tttgtcttgc
      781 tttcagtact gctggtgcta gttttgttgg tatttgagtt gtttgaagca tcagaggtat
      841 cttttggcaa atatagacta aaccgaatgt catcatttac taagtcgatt ttatctgctt
      901 taatatacat cccgttttgc attcggaatt gatccaacct aagcatgata gtcagatcat
      961 ctgtattgac ttcgatccaa tctggtaatt tatagtttct cttgatcata ttcatcacat
     1021 ctttgattgg caaactcaat gttccgatag aaagacttct agcttttagt tggacatttc
     1081 cgttttccat cacatacgga tcaaaatata aatagaaatc gataggaaaa cctagtactt
     1141 cgaaagtacc attgagtagt gcttcatttt ccaaataaaa tttatattca atatcactat
     1201 ctttttgata ctcactaagg aaaaagctga tgatttgatt gactttttct ttatttgaat
     1261 tgatcgttaa gacaggatct ccttgtcttt ccgtaagagc tggtatttct gggagttctg
     1321 gttcccgatt agcaaaaata cggctgccta aaaatacggt cgaaccaagt actaccccaa
     1381 gaaggataag gaaggcgaat ttccagtaat ttgttttctt tgttttgttt gaagatacgt
     1441 cagatcttgt tttcatgttt attgatcatc ctccttctct aaccattggt tttgtgtctc
     1501 gatcagctga tctctcactg catttgccat caattgataa cctaaattat ttggatggaa
     1561 atgatcttct tcatagagca cattattttt gatgccattt agattttctg tcgtgctagt
     1621 tgtttgatct gttccttctt ctgtgacacc tacttcctga ttcaatcctt gataaagcaa
     1681 atcattgatt gggataaagt agcagttcgg catttttttt gtcaccgttt ctgttccttt
     1741 gttccaattg ttaacaattg tctgcatgtc agtgatctca ggaaagttca aataaaatgg
     1801 attgtagatg cctaaaatgt aaataggtgc ggttttattg agggatcgaa attcttccaa
     1861 tagttctgtg acattttcct gatagttttt cagtggcttt ttaaaggaat tgaccgttaa
     1921 cccgaataaa ttattttgga taactttcat caaatcatta ccgccaacag tcagtgtgat
     1981 aaaatcagct gttttgatat tgtttcgaat ttcttctttt gtttttactc gctttaagat
     2041 ctggtcactt cgttcaccat ttacaccata gttttctgtt tcaaccgcgg tcaaattata
     2101 acggtctttt agatcatttg cgacaagggg aacgaatcct ccttgcttgg tttgatctcc
     2161 tactccttca gtcagcgaat ctccgatagc tatgtagtga agtttttctt ttttatttgg
     2221 atcttctctc acagtagtta ctggtttcat tagtgggcca gctttcggta tagaagcagc
     2281 taaacctgca tagatgatga tcgcaattaa taatgggaac aaaacgagga tcaaatgtcg
     2341 tttcgttaat ttcatttttt cacctcttgt attcgattgg ttctaatgtt atcaaaaatt
     2401 ttatcaaaaa ggtagtcttt atgaaaaaag aaaacaatta ttttcctaat tttataaaga
     2461 aaaagagaat aagctgcaag cctttcaggc tgagggctta tcctcttttc gactaatcta
     2521 cataatacat gattgcaaac gcgtctttac cagtatgcgt agcgataacc ggattcgtat
     2581 gcagtacagg gatatgcatg tccgggaata attcttgcaa accctctttg aattcttgcg
     2641 ccaattcttt tgcatccgca tgtgaaatac cgatttgtcg cacatttggc aattctttca
     2701 gttcattttt caaattatta aaccatttat cgaacgtttt ctttcctcgc cccttagtta
     2761 cagggattaa ttcagaatgg gcgaaattca tcaccacgcg tatgttcaaa acactagaca
     2821 gcaaaccagt tgttcggctg attcttccac cttttaccaa gttatctagt gtggatattc
     2881 cgatgtataa ttttgtattg tcttgtactt ttttgacttc ttcaaggatt ttctctactg
     2941 ttttaccttc ttttgctaat tcagctgctc gaattacctg aaaagataat ccttggtcag
     3001 tggtatcact gtcgataacc gttactttcg tttttgttag attactggct tgtcttgctg
     3061 cttctactgt tccactcaag ccttttgtca tatggatcga aatcacttca ctgccgtctt
     3121 ctcctagctc atcatacatc gagatgaatt ctccaattgg tggttggctt gttttgggca
     3181 gattttttgc tttcgccatc atttccataa atctttcgcc gtctagttgg tcatcatcgg
     3241 gatatactgt tccgtcgatc atgatggata gcggcatgac acgaatgcct aaagcatcgc
     3301 gtacattttt ttccatcgta catgaagaat ctgttacgat ttttacattt gtcattattt
     3361 tctctctctc tttctttaac aagatgcctt attcatgtta aactacatgt aagacaattt
     3421 ctatttttag tataacagac aaataatgat ttttcattaa ttacacctag taaaggagcc
     3481 gttttcatgg aaaaaacgaa atttaatcga acgtatgaaa tattaaatga agtgtttaat
     3541 gcagtcaccc atggaattgg gactggattg agtatcgcgg gactagtggt tcttttagta
     3601 aaaggtgccc acttgcattc ctccctccat attgtctctt atgctatcta tggatcgatg
     3661 atgattcttt tatttttaac gtctactttg tttcattcat tgatttttac aaaagcaaaa
     3721 aaggtgttcc aagtattcga tcatagttcg atttttcttt tgattgcagg aagttatact
     3781 cctttttgtt tgctgagtat ccgaggatgg ctaggttgga ctttatttgt attaatctgg
     3841 ttgtttgcga ttcttggcat tatatataaa gcgttgacgt tgcataaacg ggagagagta
     3901 tcaaaagtat ctacgatcat ctatattttt atggggtggc tctgtgtcgt ggctgctgtt
     3961 cctctttatc actctcttgg aatgactggt gttattttga tggtagcagg cgggctttct
     4021 tacacgatag gcgcattctt ttatagcttg aattctatcc gctatatgca tgttgtttgg
     4081 catttgtttg ttatgttagg tgcaggtttt atgtacttct ctgttttatt ctttacttaa
     4141 taaaaaaaat aaagctatgg atgggacagc tctaa
//
LOCUS       DAPCMU010000184         4158 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPCMU010000184 DAPCMU010000000
VERSION     DAPCMU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.189"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(100..912)
                     /locus_tag="RD744_002591"
     CDS             complement(100..912)
                     /locus_tag="RD744_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558554.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1075..1182
                     /locus_tag="RD744_002592"
     CDS             1075..1182
                     /locus_tag="RD744_002592"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558555.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1240..2307
                     /locus_tag="RD744_002593"
     CDS             1240..2307
                     /locus_tag="RD744_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0558556.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2449..2652
                     /locus_tag="RD744_002594"
     CDS             2449..2652
                     /locus_tag="RD744_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDU0558557.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            2712..3914
                     /locus_tag="RD744_002595"
     CDS             2712..3914
                     /locus_tag="RD744_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0558558.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1384 a          664 c          871 g         1239 t
ORIGIN      
        1 taacaggggg ctaacaaaga acaggaaact gtgacaacta cccattgggg agtgtcctag
       61 cagtattttc tttgtcacgt gcttatcaat tttgtcacct cattttgatg atggatacgc
      121 tgcatttaac aggcgttctt tctcgacttc catcacgaat ggtagataaa gatattgctg
      181 ttctagtaga tattgattgt cctctgtgta ctgataagaa gcggtttctt ctgaatagac
      241 caacggggca attcgcacat cgctggtcaa ttgattaatt gagtatagaa ggatggcttt
      301 ctcacctagt tccttgggta aatgtttccc aaaataggaa taggattttt gatttgctaa
      361 atagacgagt atgcgatcca cactaaagtt ggagaaggta aagggcatca tgtgcagaat
      421 atgattttga ttatcatgtt gcaaatagag ctgggcttcc ttaccaaaat tagttgaacc
      481 aagataaagc tgcttttttt catcgataca ttggaaagta aactggtctt gttcagttgg
      541 tggaatgaga tttgtaataa aaaattcaat cattcgatcc atttcttttt cactaacaag
      601 gtcagcgtgt ttgatttcgt ctactatgag accttcaatc ttaggatcaa gcttacctgc
      661 ctttttttgg agcatttcta aaatgtattg attctcttga tcaccataca aaagatattc
      721 attggtgaca tgacctatct ctgcgatttg gtgaagggtt tcggtttttg gaaggttgat
      781 cccacgttcc cagttattga ctgtacttcg cggtaaatca ccaattagtt tgccaaattt
      841 ctccatactc aactttaatt cttggcgaat ctgccgaatt cgattgccga ctgcttgctt
      901 gtctatctcc atgaaaactc ctcctcagga taagttctta aatttagtat acgtgtgtac
      961 ttaaaaaaag tcaaaacata ttgacttaaa ataaatacag gcgtacaata aaaatgtact
     1021 taaaataagt tcaataggtt ctttgacaag taaatagcag aaggaggttt gtcgatgaac
     1081 caagttaatc aacatgatct cggtgaagcc attcgagttt ctcgagaaga aagggggtgg
     1141 acacaacgat atttagcaga aaaagtgggg atttcgagat agttattatc gaaagtagaa
     1201 aaaggaacga gacaattaag tgaagaaaaa ttgaatcttg tgttggatag tttgcaagaa
     1261 gaggtcgttc cagttaaccg tgttttgatt gattatctaa ccattcactt tttctcgaat
     1321 caacatttaa agctaattga agagattatt gaaatgccca ttgaacgttt tgaggaactg
     1381 gattatgcgc caaagggcta tattggccaa tacgtgtgga atcaagtaat cacgattcgg
     1441 tattccattg acgatgcggt taaaggaact gtgatggagt tttcaggtca aggctgtaaa
     1501 cacttagcaa gatggctaaa aacacgaaaa acaaattggc aacagttttt tcgtaaggta
     1561 ttagattatc aggggaactt tacacggatt gattttactt tagatgattt tgtggggagt
     1621 ctcagcattc cagaacttaa gcataaggta atactaggcc atgtgtggac cacttttcaa
     1681 gtgagtgaat ctcatggcgg tacggatatc ataaacaatg aatcaaatgg tgagaccttg
     1741 tatttaggct caaagaagag tcagtgtcgt ttttgctttt atcagaagga ccatgaacaa
     1801 cggaagcgac gaggaattcc tttagaagag gcaaaagtaa agaatcgctt tgaactacgg
     1861 tatcgaaaag agaaagcgca aagcttggca aagattattt caaaaaccca tgatttaacc
     1921 aaactgttct ttgagttact taatggagca atttgttttt atgaccgtga tccaaatgat
     1981 ctaggtgcaa aagttgatgc caaatgggca gcctttatta gcaattatgg cgcaatcacc
     2041 atttctttag atacaattcc tcaaagtttt gagaaaacta tgagttggtt aattcatggt
     2101 gtttcaccat cattagtttt tattgcacag gtcgatcaaa cgtttcattc ttccttgttg
     2161 cctttgatta ttgaacaagg agaaatgaat ccaagacaga tcaaacttct ccaaaatatg
     2221 aaggcagacc cggagtatta tcaggaggag gttgagtatt atgaacgaaa attacaaagt
     2281 atatcaaatg agaaaacaag ctactaaaaa agcaaagtac tgcttacaca acaagttgtg
     2341 caacgtactc cgcctcgaca agacgagtat aacacaacca tcgaatgaga aacaatagat
     2401 tataaagagg attattttaa aaggagtaaa gaatcaggag gtgagagaat ggaaacaaaa
     2461 tgtgatttca tggtaaatcg tgcaattttg atagagatgg gctttaaacc tagtcaagct
     2521 gcacgaatga ttaaagagag caaggcatat cttgcaagga ttgaaggtat tgatttttat
     2581 aataatcgcc aagttggtgt ggtgccatcc cgagtaattg aacatctttt tcacattcaa
     2641 gttgcggaat aaacagtaca agcgcggtac tattaaagta gcttttctca agtacaggga
     2701 ggaatggaac gatgccaaaa attaaaaata tttaccaaga taaaaagaca aaaaaatggt
     2761 tcttccgagc gtatctaggt atagatgaaa atggaagaaa ggttcagaag actaaacgag
     2821 gttatgcttc acaaaaagat gccaaaattg cttatgacaa gtacatggaa acacatgatt
     2881 ttaacaagac aattcctaat caactgtgtt ctaccaatca aatgactttc gaagagtttt
     2941 acaaggatag gtttgtaaaa tggtacgaga gacaggtcaa aagtcaaaca tatgagaatg
     3001 cgcaattcat tttcgaaaag cgtatgcagt ttttctatca ttttcgtgtt cgagatatta
     3061 caagtcacga tattgaggat tggttatttg aactaagcca gacagagtca cgtaattcac
     3121 ggaaaaaagt taatggcgaa acgttatcaa aatcttatat caatcggatt cgagggcacc
     3181 taaaaattgt attggatcgg gctgttaaag agggggtgat tgctaaaaat cccgttgaag
     3241 atgtttcttc gcttccttct gagaataaga aagttgagtt ttgggaacac agtgagtttc
     3301 aaaaagtgat gaatgaattg aaggatgata agattcaaac ccaccatcgg aagattgtgt
     3361 atgaaatgct cttttatact ggcattcgga ttggagaact tgaagctttg tcttggtcga
     3421 atgtagattt tgagaaaaac acgataacga ttgagaagac attgatttat aatacaaaag
     3481 atgattggta cttttcgact cccaaaacta aatgtgctta tcgaacgatt gggttgggga
     3541 agaagctttc cttaaaactc agacagtggt atgacttgca aaaacgtatt ggaaattttg
     3601 aatatgttgc tcagttggac ggaacattta cgccacctta ttcttttgcg aactggttaa
     3661 aagaagctgc taaaaaagcg ggtgtcaaac cgattaagct tcacgcgcta cgacattctc
     3721 atgttgcatt tctaattgag cagaatattc aaccgctatc tattcaagaa cgattgggcc
     3781 atgcgaatat tcaaatcact ttaggaactt acggtcactt atatgcaaag tcagatcaac
     3841 aagtggtaag tgcgattgat aatgttcaag agggcgctat tctttcaaaa aatgacaatt
     3901 tattaccagt ctgagttttc cacggttcta ccataagaca aataaatgct tttgcaccag
     3961 tgttttcagt cgaattgctc ctgttcaaga tgtatcggaa atagcgagaa aataagcaaa
     4021 tttgcataac tataataaaa aggacttagt tttgatggga aatttttcaa aattaagtcc
     4081 ttttttatgt ggttgaaacg ggtatgatca ttttaaggaa gtgatctaag atgaaaggac
     4141 gactgaacaa ttatcata
//
LOCUS       DAPCMU010000185         4123 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPCMU010000185 DAPCMU010000000
VERSION     DAPCMU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.190"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            179..2107
                     /locus_tag="RD744_002596"
     CDS             179..2107
                     /locus_tag="RD744_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDU0558559.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="RD744_002597"
     CDS             2245..2700
                     /locus_tag="RD744_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDU0558560.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="RD744_002598"
     CDS             3170..3439
                     /locus_tag="RD744_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558561.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="RD744_002599"
     CDS             3839..4093
                     /locus_tag="RD744_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDU0558562.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT         1305 a          727 c          780 g         1311 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta ttt
//
LOCUS       DAPCMU010000186         4097 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPCMU010000186 DAPCMU010000000
VERSION     DAPCMU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.191"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(49..528)
                     /locus_tag="RD744_002600"
     CDS             complement(49..528)
                     /locus_tag="RD744_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDU0558563.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            887..1669
                     /gene="rpsB"
                     /locus_tag="RD744_002601"
     CDS             887..1669
                     /gene="rpsB"
                     /locus_tag="RD744_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDU0558564.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1768..2649
                     /locus_tag="RD744_002602"
     CDS             1768..2649
                     /locus_tag="RD744_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDU0558565.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2785..3507
                     /locus_tag="RD744_002603"
     CDS             2785..3507
                     /locus_tag="RD744_002603"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDU0558566.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            3510..4067
                     /gene="frr"
                     /locus_tag="RD744_002604"
     CDS             3510..4067
                     /gene="frr"
                     /locus_tag="RD744_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDU0558567.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1464 a          726 c          889 g         1018 t
ORIGIN      
        1 attgaaaata aaaaaacacg cattccatat taggaatacg tgctctgatt aaaaaagtga
       61 tacttcagtt ggatggtgaa gaaattcttc tactcgtttt gcgtcctcct cattttccga
      121 gatgaccatg atcgtatcga atccagcaat tgtactgaca atagagggag gtttgatctc
      181 atctaaaaca gatgcaaata aatgagcatt gtctggcaag gtattgacga tcgtcaaaaa
      241 gtggactcgt tcgactttca atacaatgtc ttcaatcatt tgaaccagcc gatcctcttc
      301 ctcagtccta gtctcaggag ttcctttttc gcgaaacaat acgaagcggg aaacgccatt
      361 ttcatcaggt gccttcacga tttttagatc acggatatct cgagaaatcg ttgcctgcgt
      421 ggcggtaacg ccataatgcc caagtttgtt taatagttct tcttgcgtac ggatcgggaa
      481 ttcttcaatg acttgtttaa tcagcaaatg tctttgagct ttgcgcattt tctttctcct
      541 ttacgaaaga tttcacatgt atcataacaa aaaaaagaag gattgcccaa taatattcgt
      601 cttttatacc taaaattatt ttatggaaaa aggataataa agaaacaaag agaaatctac
      661 tgtttttctt gaattctttt tatctttcag gtaaactatt gaatgttgag cagaaaagta
      721 tatttgctct tgcgatacgg atcttttgtc tgtataatag ctgagggtgc tttatgcatc
      781 agcaaatcca cattcttatt ggattgtcgg taggtgcttg aaaaagtttg ccggcaaggt
      841 agaagataag aagaggaaaa ataaaaccaa atcggaggaa acaaacatgg cagtaatttc
      901 tatgaaacaa ttactagaag ccggcgtaca ctttggtcac caaactcgtc gctggaatcc
      961 aaaaatgaag aaatatatct tcacagaaag aaacggaatc tacatcattg acttgcaaaa
     1021 aacagttaaa ttagtagatg cagcttatga ttacatgaag agcgttgctg aagaaggcgg
     1081 cgtagcatta ttcgtaggta caaaaaaaca agcacaagaa gcaatcaaag aagaagcaac
     1141 tcgtgcagga caatacttcg ttaaccatcg ttggttaggc ggaacattga caaactggga
     1201 tactatccaa aaacgtatca gccgtttgaa acaaatcaat gcaatggaag aagatggaac
     1261 ttttgaagtt ctacctaaaa aagaagttgc tggtttgaac aaagaacgtg aacgtcttga
     1321 aaaattctta ggcggtatcg ctgatatgcc aagaatccca gatgtaatgt acatcgttga
     1381 cccacgtaaa gaacgtattg ctgttcaaga agcacacaaa ttaaatatcc caatcgtagc
     1441 tatggttgat acaaactgcg atccagatga aatcgacgta gtaatccctt caaacgacga
     1501 tgcaattcgt gccgttaaat tgattacttc aaaaatggct gatgctttca tcgaaggaaa
     1561 ccaaggtgaa gatcaagtag ttgaagaaga tttcacagaa gaaaacacag caacatcaat
     1621 cgaagaaatc gtagatgttg ttgaaggcga caattcttca gcagaataat tttgaatgat
     1681 ggagctgttt caaaggctgg gcgacaagcc tcactctcct ttgaagcagc tttttttaaa
     1741 aagaaaaaca cacaggagga gttaaacatg gcagacgtta cagctaaaat ggtaaaagaa
     1801 ctacgcgaaa tgactggcgt aggaatgatg gatgcgaaaa aagcattagt tgaagtagaa
     1861 ggcgacatgg aaaaagcagt tgatctttta cgtgaaaaag gaatggcaaa ggctgcgaag
     1921 aaaaatgacc gcattgcagc tgaaggattg gcttcagttg ctgtgaaagg taacactgca
     1981 gcaatcgttg aagtaaactc agaaacagac tttgtttcta aaaacgaaat gttccaagat
     2041 ttagtaaaag aaattgcaga acttgttgct gaaaacaaac cagcagacat ggaagctgca
     2101 atgaaaatca aaacagataa aggtacaatt gaatctgact tgatcgaagc aactcaagtt
     2161 atcggagaaa aaatcagctt ccgtcgcttt gaagttgtag aaaaagacga caatgctgca
     2221 tttggtggat acttgcatat gggcggacgt attgccgtat tgactgttct agatggaaca
     2281 actgacgaaa cagttgcaag agacgtagct atgcacgtag cagctatcaa tcctcgttat
     2341 gtaaacgaat cacaaattcc agaagctgaa ttagaacatg aaaaaactgt tcttacagaa
     2401 caagcattga acgaaggcaa accagcgaat atcgttgaaa aaatggtaga aggacgcttg
     2461 aagaaattca aagcagaaat cgctttggtg gaccaaccat tcgttaaaga tcctgatatg
     2521 actgttgaaa aatacgttgc atcaaaaggc gcaactgtta aaacattcgt acgtttcgaa
     2581 gttggcgaag gtatcgaaaa acgcgaagat aactttgtag aagaagttat gagccaagtg
     2641 aaaaaataat tctgttgaat aacagacggg aacgcacggt ttgtgcgctc ccttttttta
     2701 agcaaaacaa gaaaaaccag tcttagagtg tcgaaatcat ctaagtatgg taaaatggca
     2761 atagaaaata ttggaggaat gaacatggtc acaccgaaat atcagcgtgt agtcttaaaa
     2821 ttgagtggag aagctttagc tggggacgca ggatttggaa tcaaaccacc agtaatcaaa
     2881 gaaatcatcc aagaaatcaa agaagttcac gagctgggcg ttgaaatggc aatcgttgtg
     2941 ggcggcggaa atatctggcg cggacaaatc ggagcacaaa tgggcatgga acgtgctcaa
     3001 gctgattaca tgggtatgtt agctacagtt atgaatgcct tagctttaca ggatacgttg
     3061 gaaaacgtgg gtgtaccgac acgcgtgcaa acttcgattg aaatgcgtca aatcgcagag
     3121 ccatatattc gccgtaaagc agaacgtcat ttagaaaaag gacggatcgt tatctttgca
     3181 ggcggtaccg gcaacccgta cttctcgaca gacacaacag cagcattacg cgcggcagaa
     3241 atcggcgcag atgttatcct gatggctaaa aacaatgtag acggtgttta ttctgctgat
     3301 ccgaaagtag atgcaaatgc agtaaaattt gaagaactga ctcatctgga agttattgcc
     3361 aaaggattac aagtcatgga ttcaactgca agttcactaa gcatggataa tgatattcca
     3421 ctgcttgtct tcaatttaaa tgaacacgga aatattcgcc gtgcaatctt aggcgaaaat
     3481 atcggaacaa ctgtaagggg gaaataaaaa tggcagatgc aattatgaca gaagcaaaag
     3541 aaaaaatgaa aaaagcagca caaaatcttc aacgtgaact tgggcaaatc cgcgcaggcc
     3601 gtgcgaacgc tagccttttg gaccgtatta ctgtgaacta ctacggtgcg ccaacaccat
     3661 tgaaccaaat ggcatcaatc caaattccag aagcacgtgt tttaatgatc acaccttttg
     3721 acaagagcat tcttcaagat gttgaaaaag ctattatggc aagtgatatc ggaatcagtc
     3781 caaccaatga tggaaatgtc attcgcttag ttatcccgca actgacagaa gaacgcagaa
     3841 aagaattagc taaagatgtt aaaaaagaag cagaaaatgc aaaaatcgcg attcgtaaca
     3901 tccgtcgtga tgcgatcgat gaatataaaa aacaacaaaa aaatggtgat attaccgaag
     3961 atgatttgcg cggattagaa aaagacgtac aaacattgac agataacagc atcaaagaag
     4021 tagataagat cgctgctgat aaagaaaaag aattattgga agtttaataa aatcataaaa
     4081 acagaggggc ggatcat
//
LOCUS       DAPCMU010000187         4024 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPCMU010000187 DAPCMU010000000
VERSION     DAPCMU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.192"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(241..390)
                     /locus_tag="RD744_002605"
     CDS             complement(241..390)
                     /locus_tag="RD744_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558568.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            852..1064
                     /locus_tag="RD744_002606"
     CDS             852..1064
                     /locus_tag="RD744_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558569.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1342..3141)
                     /locus_tag="RD744_002607"
     CDS             complement(1342..3141)
                     /locus_tag="RD744_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDU0558570.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3265..3861)
                     /locus_tag="RD744_002608"
     CDS             complement(3265..3861)
                     /locus_tag="RD744_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDU0558571.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1448 a          710 c          688 g         1178 t
ORIGIN      
        1 tattaaatat tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc
       61 ataaaaaacg agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga
      121 gagacaattt ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta
      181 caaacgtctt ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc
      241 ttatctagat tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt
      301 attttcgttt tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg
      361 gtattgcata tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag
      421 ttcttgaaag tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt
      481 gatgcaatta aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta
      541 ttttctttta atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata
      601 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
      661 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      721 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      781 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      841 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      901 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      961 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
     1021 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
     1081 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
     1141 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
     1201 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
     1261 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
     1321 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
     1381 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
     1441 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
     1501 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
     1561 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
     1621 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
     1681 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
     1741 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
     1801 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1861 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1921 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1981 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     2041 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     2101 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     2161 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     2221 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     2281 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     2341 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     2401 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     2461 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     2521 ccgaatggct gctcgaaaag gacgatctcg tagaatcatt tccggtaaag caaaaatcag
     2581 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     2641 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     2701 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     2761 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     2821 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2881 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2941 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     3001 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     3061 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     3121 gctacgtttc aaataattca tcctatcacc tgctcttcac attgtagcgc aaatctaatg
     3181 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     3241 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     3301 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     3361 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     3421 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     3481 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     3541 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     3601 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     3661 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     3721 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     3781 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3841 tcgagaggca gcaatattca ttattcatca actccttaga ggttctttgt taactatact
     3901 ttactagaaa agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt
     3961 aagttcatca taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta
     4021 ataa
//
LOCUS       DAPCMU010000188         3994 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPCMU010000188 DAPCMU010000000
VERSION     DAPCMU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.193"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            402..734
                     /locus_tag="RD744_002609"
     CDS             402..734
                     /locus_tag="RD744_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558572.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            859..1116
                     /locus_tag="RD744_002610"
     CDS             859..1116
                     /locus_tag="RD744_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDU0558573.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            1348..1752
                     /locus_tag="RD744_002611"
     CDS             1348..1752
                     /locus_tag="RD744_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDU0558574.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1771..>3994
                     /locus_tag="RD744_002612"
     CDS             1771..>3994
                     /locus_tag="RD744_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDU0558575.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     M"
BASE COUNT         1475 a          529 c          727 g         1263 t
ORIGIN      
        1 cgttatgtag taaatgtaat agtttttttg gtagtgagta tgttgaggaa tatattagat
       61 ttgcagaaga caataaaaat ttcctttata acaatacaag tttaaagaat ggtaagtctg
      121 atttaacaca ttcaataaag aaaatgaatt cacttagagt tgcaaaagca attgtaggaa
      181 tgttttttag tttaaatggg gatgaagatt caatggataa gccattctta gattcggtac
      241 gagaatatct tagcaatcct aaaagtactt tatttcctca tgaagactac aaaattataa
      301 tgaattatta tcttccaata ggtgctccac agaattctaa aatcaaaatt cctaatgaca
      361 ttgtaaatga tggtgacact cacaatgtaa taaaaggttt tctgcccctt gatctatcaa
      421 ctgggagaaa aattcgattt tcagaaattc agtatgaagg aattggtttt actcttatag
      481 atttaaaaaa ttcaactttt gaaataaaag aaggattaga tttaaaaaat ctcctttgtg
      541 cagatgataa aatcaaaaaa atacaattgt acaaagtccc tatattaaca ccaacagtac
      601 ttacaatgtt ggagatggaa aataatatta attatattga ctcaggttca gatttgctga
      661 ttaggtggca ggtaataact aattttctta tggaattaaa aaaatacagt aatactcaaa
      721 cgaagttctc ttgaagttat aaagaataca ataaatataa atttccttta gactatatta
      781 aaatcagcaa tcgaaggaaa ataaaagaag aaaatggtac gcaaaatcta actattagag
      841 caaaaacagg aagctgcttt ggaagatagt cccgaattgt ttccgatgtt aaaagatcgt
      901 ttaaatgaat tgactgaaaa acgaagacag ttacaaattc gagagaatga aatcttaggg
      961 atctttcaac aacaagggga accgattcaa gtcaaagatg tccaacagat attgactagt
     1021 ttggattgct ttcttatcca gagtgagaag aagcaagtta aagctttgta tcacaccttt
     1081 attgagaaaa tcacctttga tcctaatcag aagtagatat tgccgatccc gatgagattc
     1141 acacctactg tgatttgata attgaatgag taatatcaaa tagccgtatc caaaccaaag
     1201 gatatggcta tttttgtgtt agggatatac gtcacgtagc ttgttatcaa gaataagttt
     1261 ctttattttt ttgctttttc taaaactgag tatgctaaaa taagagggta tatagcaact
     1321 ttaaataaga gataggagtg tatctttttg ggtgcagaat taaatcagaa gttatttagt
     1381 gcggcagata atctccgtag taaaatggat gcgtcagaat ataaaaatta tttattgggg
     1441 ctgattttct ataaatattt atccgaccgt ttgttggaac aagtggttct tttagcagat
     1501 gaatccttag aggaatatga cacagtaagc aaacaaacga tgttgtatcg tgaattactt
     1561 tcagatgaag aatcaaaaga agatctgatt gcaacaatcg ttgatatttt ggggtatgcg
     1621 atttcgccag agtatctttt caacgtctta gcggatcaag caaaacaagc aacgttccaa
     1681 ttgaatgatc taaacaaagc ctttttagca agtcatattt atggaaaaat actgtcggca
     1741 aaattcgaat agtgtcgagg aggtgctgtg atggaagaat taaaagaacg cattgtcaat
     1801 tatgtcatag agaactctga gattaaaaat aaatggggga acaatacagc acagaaaata
     1861 tctgaaaaat taaatgttag tcgtaatatt gtctctcaat atttgaatga attcgtaaaa
     1921 gatggagaat ttggaaaaat caatactcgt ccagtaatct tcttttcttt accggagaaa
     1981 gttccaaatc aacaaatttt tagctcttca gaggaagcgg aaaaatggta tgatagctct
     2041 tttagggttg gaatagaaag ggttatcgga agtcaaggaa gtctgcgaaa tgtaattgct
     2101 caaattaaag cagcaatcta ctatccgcca aatggcttac ccattttact tcatggcgag
     2161 actggaactg gaaaaagcta tttagcaaat tatgtgtata aggatttgtt ggccaataat
     2221 tatatagaag agaaggcatc tttcatttca gttaattgta gtgaatatgc caataatcca
     2281 gaacttttct tgtccaattt ttttgggcat gtaaaaggtg catatacagg agccgaagat
     2341 ggaagaaagg gtttagcaga attagctaat aatggagtct tatttttaga tgaaattcat
     2401 gcattgagca atgaatgtca ggagaaactt tttcagttta tggatacgag gaaatttcat
     2461 cggatgggtg ataatgaaaa ctggtacaca tcaagttgct ggattatatt tgcaacatca
     2521 gaaccacctg aggataaact attatcaact ttgttgagaa gaatacctgt aaagctaaca
     2581 cttccaacat tagacagtcg ttctctttat gaaagaaaac agttaatcaa gatatttttt
     2641 aaatcagaag aagaaagact agcaaagaaa atactgatat ctcaacaatt attagatttt
     2701 ttattaaact atcgctttag tgaaaatgtt ggcaatttga aaaatatcat tcaattgacc
     2761 tgtgccaatg ccttttattt cttagatgat ttggaagaac tttatattca gatgtatcat
     2821 ttaccacaat gggtgaattt caacggcatg aaggttatga aaagacagaa tgaattaaaa
     2881 tatattcaaa tggatgattt ggaaaaagta gacgaagttt ttaatatcaa cgaacttata
     2941 gagtatcttt atcaaacgtg gaagaaagac actgataagc agcagctttt tgagaaatat
     3001 gatagatatt ttagacaaaa gaatgttgat atccaaaata attcagcttt aacatatccc
     3061 acctatcaag agattcgatt tagacagtat gttaaggaaa tagagagaga ttttcaaata
     3121 gaatttgact attattatct tcaaataacg tctatctttt tgaattcttg gatttatcaa
     3181 atccaattaa catcggacga taaattatat tccttcatca atgatattaa tagttggtgt
     3241 agtcgagaag ttcaattagt caatcgtttt attcaaatag gggatattta tttaacaaaa
     3301 attcaaaaaa atgttttggt tattttattt aagttaatgt gtagtaaaga atttcagcaa
     3361 aaacgtttag cactgatttt ggctcatgga gatctaacgg caacctgtat agcaagtact
     3421 gctaatagga tcgttgagag atatatatac gacgccattg atatgccact aatgacttca
     3481 agtagtgaga ttgcagacaa aattaataga tacttacaag agtgtaccgg atatgaagaa
     3541 attattttac ttgttgatat gggttcactg gagaaaattt atctaaattt ggaactagac
     3601 gctcaaaaaa aattcggact actaaacaat gtttcaacaa gaatggctat tgatgttgca
     3661 aatcagatat tattagattt accgcttaaa cagattcttg aacaagtaaa agaaaatcaa
     3721 aatgtggatt atatttatga atcatcaaat attaagaagg atatgattgt ctgttcttgt
     3781 gcaagcggat taggaacttc gtttaaatta aaaaagatac ttgaagatag ctttccaagg
     3841 gatactgatc ttgtcataga aacgtgtgac tattactctt tagtgaatga aaactattta
     3901 tcggagcttc gtaaagagaa caatattctt tttattgttg gaacactaaa tccacatcta
     3961 gaagatgtag aattcttttc aattgaagat atga
//
LOCUS       DAPCMU010000189         3925 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPCMU010000189 DAPCMU010000000
VERSION     DAPCMU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.194"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(573..1130)
                     /locus_tag="RD744_002613"
     CDS             complement(573..1130)
                     /locus_tag="RD744_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558576.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="RD744_002614"
     CDS             complement(1309..1920)
                     /locus_tag="RD744_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558577.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="RD744_002615"
     CDS             complement(2033..2290)
                     /locus_tag="RD744_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558578.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="RD744_002616"
     CDS             complement(2887..3165)
                     /locus_tag="RD744_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558579.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="RD744_002617"
     CDS             complement(3199..3450)
                     /locus_tag="RD744_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558580.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAPCMU010000190         3891 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPCMU010000190 DAPCMU010000000
VERSION     DAPCMU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.195"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            51..1241
                     /locus_tag="RD744_002618"
     CDS             51..1241
                     /locus_tag="RD744_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDU0558581.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1375..3564
                     /locus_tag="RD744_002619"
     CDS             1375..3564
                     /locus_tag="RD744_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDU0558582.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3561..>3891
                     /locus_tag="RD744_002620"
     CDS             3561..>3891
                     /locus_tag="RD744_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDU0558583.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRV"
BASE COUNT         1380 a          616 c          717 g         1178 t
ORIGIN      
        1 tctgtagaat agagttacca caacacattc acagaagaga ggacttccct atgaacgatt
       61 ttactacaga aattctaaag actctagcga acaaaggcga tttgaatgaa ttattccgtg
      121 tccatttgga gaaagcagtc aatacgcttc tcaaaacgga gttaacggct ttcctagatt
      181 acgaaaagta tgatcgcatt ggttttaaca cgggtaattc tcgtaacggc tcctatgacc
      241 gtacggtcaa gaccgagtat ggggaacttc atctccagat tccgcgcgac cgcaatggtg
      301 agttcaagca acagactgtt cctgcttata gacggacgaa tgacacgtta gaggagaccg
      361 tcattcacct cttccgaaaa ggtattacca tgtcggaaat cgcagacttg attgagaaaa
      421 tgtatgggca ttactacacg ccccaaacca tgtccaatat aacaaaatca tttacagaag
      481 aggtaacggc gtttaatggg cgggagcttc atgaccgtta tgctgctatt tatatggacg
      541 caacgtatat tccgttaaag cggaaaaccg tcgccaagga agctattcat atcgcagttg
      601 gcattcgccc ggacggatca aaggaagtat tgagctatgc gattgcaccg actgaatcca
      661 tcacgatttg ggaggaaatt ttattggacc ttcaagagcg cggtttgaaa aatgtcctcc
      721 tgttcatcac ggatggctta aaggggatgg taggagcgat tagtcggttc tatcccaaag
      781 ctcgttttca acattgttgt gtacacgttt cccgtaatat cagtcataaa gtgcgtgtcg
      841 atgatcgtaa ggaagtctgt gatgatttta aaatggtgta tcaagcctca tctaaagagg
      901 tggcgttgga agcacgtggt acttttgcgg aaaaatggaa aaccagctat ccaaaagtgg
      961 ttgaatcgat tctttcgaac gatcacttgc tcactttcta tgatttcccc ttggccatac
     1021 gcaagagtat ttattctacg aacttgattg aatcctttaa taaacaaatc aagaaataca
     1081 gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta gtctcgtctt
     1141 ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa caggccgaag
     1201 gcgagctcga acaaatgcta agccaactga ttgagaatta gaaacaatct gcagtaggga
     1261 agaatcattt acacaaaatt attgacgctc ccttataatg agaactttta ctaacataat
     1321 ttatgataaa cttactatat aatcatatta attaaatagt taggagatct gcttatgttt
     1381 atatctactc ttcaaattcg aaattttaga aattatgaat ccgaaatctt tcgattcgat
     1441 aatgagacaa atactattat aggtgagaat gattcaggga aaaccaatgc tcttactgca
     1501 ttgagaatct tattagatga tagttattac tatagtagta aaacactaaa agaatcagat
     1561 ttttttcatg gaattcaaaa tgggtggcaa gggcattgga ttattatctc tgcgacattt
     1621 gaaggaatca gcgaagaaga atttgacaat gagatttgtg cttccatttc tttaaattct
     1681 gaaagtcaaa caatcttaga agaattgatt tcaaatgcag ataaaggtgt aggtagtatt
     1741 tctctattta ttagacctaa caaagctatt agaaagcaat tatttgatat ttccactgaa
     1801 ttagactctc atcaatttaa cgaatttaga aattctattc gtctatccga ctatgaattt
     1861 tactacacat ctaaatcaaa tctaaatttc tgtatcaatg aaaattatga taaattggta
     1921 ggaagactca atgacaatat agctagtaat cctgaagaag acgatgaagc tctgttgggc
     1981 tcaagaattg acatgtcaga tattttcaag catatttcgg ttgtttatgt tgacgctctt
     2041 cgagatgttc ttagagaaat gaaaaacaat cgaaacccag tcaaaagaat catggaaaca
     2101 attgaatcaa aaatatcatc agataatgtt gagtcattaa aaacaattat acagcaatta
     2161 aatgagacta ttaccagtgt tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa
     2221 ctaaatagca taattggttc agtgtattct cctaatcttc ttctttcctc aactatgtca
     2281 gatgatatgg gttctttagc aaaatttctt tcaatgaagc cagaacaaaa tatcgactta
     2341 gacttactag ggttaggaca tctaaatatg atttatttag ctttgaaaat agtcgaattt
     2401 gaagcgtgtc gttcaagaga actacttaat attatgttga ttgaagaacc agaagctcat
     2461 attcatcatc atattcaaaa aactctgttc gaaggattaa atcttcaaaa aaactatact
     2521 cagattctca tgaccacaca ttctgtacat ttagctgaag tgtctgaaat atctcggatg
     2581 aatgtattaa aatcgtttaa tggcaaatct atcgttatga atccagcaaa taatctgaac
     2641 tcatttgcta aagaaaagtt aaaaaagaac aatctaaatc taataaacgc agtagagaga
     2701 tatttagatg ttaaaagaaa tggattgctt ttttctaaag gtgttatctt agttgaagga
     2761 gatgctgaag aaatacttat tcctcaaata gtaaaaaaat tatttggtat aaacttagac
     2821 gaacttggaa taggtttgat taatgttggt agtacttcgt ttgaatatat cgctagcttg
     2881 tttgacgatg atagaataca gagaaagtgt gctattgtaa ctgatttaga tgaacaggca
     2941 atcccatcag aacatcgatt gtataaaagc actgcacaag agaaaggtca agcaagaaaa
     3001 gaaaaattat cctccctttt taatgaaaac aattgggtta attgttttta tgctaataca
     3061 acatttgaaa ttgagttttt agatgtgaat aaagaaaata ttctaactta tgtcagtcca
     3121 attatagatg acgcattcaa agataaaaaa acaataactg cttataaaga agaaatacaa
     3181 agtgaacaat atttagaaag aaatttagcg atgttaaaac tagccaatca tgttggtaag
     3241 gggtggtttg caattgagtt aggtgaaaaa attgactttt taatcaaaat tcctaagtat
     3301 attcttaatg ctattgcttt tgcatgtcag gaaatagtag atctctcaat ttataaaaaa
     3361 attctactcc atacaatcga cttgtacgag gaaactacag aattgtcagc tttatcagct
     3421 agtttaaaaa caagtgatac agctgatttg aatatagcaa taatttcttt attagagtac
     3481 tttggtaaag atgaggatgt tgtgtatcta atcaatcaga tagaaaatta cacaaaaata
     3541 ctttatgctg aggtttcaaa atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt
     3601 tagaacaaaa agatgttgtg gaaagtgaag ataaccttta tgtaaatgca tgtcctggga
     3661 gtgggaaaac aagggtactt acaaggaaaa ttgcttacca agcaattaaa cacgatggat
     3721 cactaaaaag aataattgct atcacataca ctaatagagc tgctgaagaa attaaggaac
     3781 gcctaagttt acttagtata gatgatgata ttaatatttg ggtggggaca atacatcagt
     3841 tttgtcttga atttattatt tatcctttta aaatgaatct ctcaagagta t
//
LOCUS       DAPCMU010000191         3717 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPCMU010000191 DAPCMU010000000
VERSION     DAPCMU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.196"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            101..2542
                     /locus_tag="RD744_002621"
     CDS             101..2542
                     /locus_tag="RD744_002621"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDU0558584.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="RD744_002622"
     CDS             complement(2872..3576)
                     /locus_tag="RD744_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558585.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1057 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaa
//
LOCUS       DAPCMU010000192         3712 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPCMU010000192 DAPCMU010000000
VERSION     DAPCMU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.197"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="RD744_002623"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="RD744_002623"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDU0558586.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="RD744_002624"
     CDS             complement(2260..2511)
                     /locus_tag="RD744_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDU0558587.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="RD744_002625"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="RD744_002625"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDU0558588.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="RD744_002626"
     CDS             3461..3679
                     /locus_tag="RD744_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558589.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DAPCMU010000193         3696 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPCMU010000193 DAPCMU010000000
VERSION     DAPCMU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.198"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            75..470
                     /locus_tag="RD744_002627"
                     /pseudo
     CDS             75..470
                     /locus_tag="RD744_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="RD744_002628"
     CDS             complement(465..683)
                     /locus_tag="RD744_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558590.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="RD744_002629"
     CDS             790..1104
                     /locus_tag="RD744_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558591.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="RD744_002630"
     CDS             1208..2386
                     /locus_tag="RD744_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0558592.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="RD744_002631"
     CDS             2789..3082
                     /locus_tag="RD744_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDU0558593.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3283)
                     /locus_tag="RD744_002632"
     CDS             complement(3170..3283)
                     /locus_tag="RD744_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HDU0558594.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
BASE COUNT         1261 a          546 c          716 g         1173 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaattt
//
LOCUS       DAPCMU010000194         3643 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPCMU010000194 DAPCMU010000000
VERSION     DAPCMU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.200"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            51..503
                     /locus_tag="RD744_002633"
     CDS             51..503
                     /locus_tag="RD744_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558595.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            517..1206
                     /locus_tag="RD744_002634"
     CDS             517..1206
                     /locus_tag="RD744_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558596.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1331..>3643
                     /locus_tag="RD744_002635"
     CDS             1331..>3643
                     /locus_tag="RD744_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDU0558597.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEK"
BASE COUNT         1234 a          475 c          766 g         1168 t
ORIGIN      
        1 cttatcgtag atatatatta taaatttaat aagaaagcga ggtgagaatc gtgaaaaaaa
       61 cgatactaag tattttttca gtatttacaa tattaattgg gggctatttt cttttgaatt
      121 tagattctgt taattcaaac gctcaaataa aagatcaaag agtagctgaa cataataata
      181 aaaaaatgag tttgaccaaa aatatctatt ctatgtcaga tgaagaattg tataaagaat
      241 ttggtgattt atccggggaa cagttagaag aaagtattcc aggaattagt gactcagata
      301 aaattgttgt cttgtctgct ggtgggcaat taatgggaga tggcaaatcc gaattagtta
      361 gttataaaca aaatcacaga cagtttattg atgttaatac tgattccaac gctatttcga
      421 ttaaggatct aaaagagctt ttaaatagta aaagaaatga attaaagcag ataaaagaaa
      481 ctaattcagt ggaattagag taaaggaaga ttcattatga aaaagatttt tggtgtttct
      541 gttgggctac ttatgatgtt aacatttgga aatgcagtat atgctgctga gaaagaagtt
      601 atttcagatc cagagcagta cgcctcacga tactttgctg attttgaaga agaagataaa
      661 gtacttgcat tgccagaagg tggtttttta aaaggggaag ctaaaattgt atctatggat
      721 aatccagata taattcaagg ttcatataat tcagaaactg atcctgcttc agtttcagtg
      781 acagaagcaa aaaaaattgt tgctgaaaaa ttcaaaaatg atattgaaac tactccaatt
      841 atgcctcgag gagcatttcc acccgatgtt ggtactagcg gacaccttct tgtactagca
      901 ggaggaggaa gctataaatc acagccattt agtggtgcgg gatggcgttt tggaggtatg
      961 ggtgtaaaac cagctgatgg tacaggcaac tttttacgtt ggaaatccta cattgacgat
     1021 ggaagagtag gaaatgtaaa tcaagccaac gcaactaaat ctggttctat tcaaggaatg
     1081 gcattgtatg ctcgtgaagg attcaaatac tttgcaccaa acgggcctgg ttggacaatg
     1141 aatataggta tttattacac ttataatcct gttgcaggta cgtattatgt tgttcaaaat
     1201 atttaataaa acagcacaaa gagatttctt tgtgctgttt tattattttt agttgttgac
     1261 aaacactaaa atataaaata taatcaacac ataaacgaac gtatgttcgg caagaaagca
     1321 ggggattttt atggatgaga tggttttaag tactcagaaa tggctaaaca agaaatacag
     1381 taatgttaca gggtttgata aggttccaga aaatgggcga acagggtggc ctacaatcta
     1441 tggacttatt gaaggactac aagtagaact tggtattact aatctagtag ctaattttgg
     1501 acctaccact gaaaaaatgt acgataatca ggttactcct aaatggggca aaaatctacc
     1561 taaaaatatt gtatttctta ttcaaggtgc cttttggtgt aaggggatta atccaggtgg
     1621 cttcgatggg gtatatactc catatttaga tactgctgta aaagagttgc agactgatgc
     1681 agggtttaat ggaagcgctg taactggagt tttggatgcg aaatgggcca aagcgttatt
     1741 tgatatgagt gcttttgttc ttgttcccgg cggagatagt aaaattagaa aaatgcagca
     1801 atggttaaat gtaaattatt tagaatacac gggaataatg ccttgtgatg gtatttatca
     1861 aagaaattca aatcaagcat taatttttgc attacaagct gaactaggat attctcccag
     1921 tgaagcaaca gggtcttatg gtaatgggac aactagtaaa acttatcctg tgtctgaagg
     1981 taactcggga aactatgtaa gaataattca atatggatta tatgttaatg gttattttga
     2041 agacggtaca ttcgatggta ttttcactaa atatatgggt ttagaagtac tggcattccg
     2101 aaaatttatg gtattgcctg aatatactga aatagcagat gttgttgtaa taaaaggact
     2161 attgtctagc gctggagata ttagaagatc tgcagatggc gcagatactt ctacgcaatt
     2221 aactagatct caaatccaaa cattggttga taatgatata aaaatagttg gtagatatct
     2281 tactggtact gttggaattg gaaaagatga acgaaataag tatctaacta cagaggaatt
     2341 gaataatatt ttttctaaaa atttatctgt gtttcctatc tatcaagatg gaggtgcagc
     2401 attagcttat tttacttatg atcgtggctt atcagatggt aagaaggcta tagatgcagc
     2461 aaaaaatcta aatataccat taacaacagt tatttatttt gctgttgatc tagacatgtt
     2521 aggggaagaa attcttgcat atgctggaga atattttaag ggggtatctg ctgtaatgtc
     2581 atatagcggg tatcaaacgg gtgtttacgg aacaagaaat gtttgttctc aaataatcaa
     2641 taacgggtat gcttcgtttg catttgtttc agatatgtcg actggttata gtgggaattt
     2701 aggttttcct atgcctagac aatggtcttt tgatcaattt gttgaattta ccataggaag
     2761 tggcaacgga gctgttggta tcgatttaat cgctacatcg ggtagagata gcggatttaa
     2821 tgagttatca aatgatacta ataatgataa ttacattgcc aaatacaatc agaaggttat
     2881 aaatcagatg gtacgtgcgt atcaatatct atcacaagct ggtcttgata atccttggaa
     2941 ccctcatttg acattttata gatatgtaaa ttattctggt ttgtcatggg acatcatttc
     3001 ttctcctgta acagagcatg atagaaaaat ttatgatgaa tataggaata aattaactaa
     3061 tagcgagggc ctgtataatt attttataga tcctaatact ggttcaataa taggtttgcc
     3121 tcacttaatt gtgacgttac aatcacagat gtttctagcc gacacaatca atgatagtgt
     3181 ttctgacttt gcaggttggt taggagattt aatgacttgc tggggtgaag ttaagaaatt
     3241 aggaatacaa atggagcaag gtgtatttgc attagtaggt acggctgcag gtggaacatt
     3301 tagtctagaa gatttatacc aagatgttga cgcggtaaat gtttatacct tagcaaaaag
     3361 aaattctgat tcaacagctt tatcggcttt aaaaacgtac tattgtgata agggtgctat
     3421 tgatagattt aatcaatttg ttaagaacag atttggttct tcagataaaa tcgcttcagt
     3481 aacaaatagt ttattagaaa atgacgaaac agttgatatc ttaggtgtag ggataatttc
     3541 atttttcatt gataaaatag ttaataattc acttgggaca gattttactt atcttgattt
     3601 tattggagat gaggggaaag ttgcaaatgg attttctgaa aaa
//
LOCUS       DAPCMU010000195         3572 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPCMU010000195 DAPCMU010000000
VERSION     DAPCMU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.201"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..641
                     /locus_tag="RD744_002636"
     CDS             <1..641
                     /locus_tag="RD744_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDU0558598.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(712..2022)
                     /locus_tag="RD744_002637"
     CDS             complement(712..2022)
                     /locus_tag="RD744_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDU0558599.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2034..3452)
                     /locus_tag="RD744_002638"
     CDS             complement(2034..3452)
                     /locus_tag="RD744_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0558600.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1141 a          730 c          560 g         1141 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata tatcattaaa
      661 aaaaataaag gtcaagctaa aatattattt aagcttgacc ttattataat ttcaacgatt
      721 gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata gataaatcac agcttttgaa
      781 acataagttt tgtactgctt cgattcgccc gaattctttt gcagttattt ggcaatgaga
      841 aaagcagaca tcttctatag gcttttcagg aaatgctact aaatcaaaag ctcgggaagg
      901 aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg atgtgtatgt ctttgacttt
      961 cgtcatttgc tttgattcag gtatttcttc agccactgct tgccaacttt ctggcatttg
     1021 agcaaggtca ttgctatttt ttctattgta ttcattatgc cagtccatta tccaagaaaa
     1081 aggaaattgt acatcaatca tattcagatt ttctaccctg atattttcga tcactccccc
     1141 tctttctttt gaagatttca ttcgaatacc acagtctgac tgaaaaaaat tgatatcgtg
     1201 aatatatacg tcactaactc cagcactaac ttcgctacca atagttacac cgtatcctga
     1261 acggatttta catctagcaa cctcgattct tgatgatttt cgattgactc ttgcaccatt
     1321 gccatctctg ccagacttaa tagcaataca atcatcacca caggccaaat cacattcata
     1381 gacacgcaca tttgttgaag aatcaatatc aatcccatct gtacttggtc catcattgtt
     1441 gcggatgatt actttttcta ctaatacatc gttagaataa gtgatttgca agttccaaaa
     1501 acctgatttc tgaaaaatca aatcacgaac aacaacatgt tctgcatgat acagtaaaca
     1561 agcgcgtgga cgtttaatag cataatccgc aatccaacga agtccctttt gatcgtattc
     1621 agctcttgtc ccgctttttt gatcttttcc ccaatacagc tcccaccaat ggtctccttg
     1681 accgtcgatt attccttttc cttctataag aatatctttc gccgagagaa cattcaaaat
     1741 cgctgctggc catttcattt ctacgccggc tactcgtgta tcgatttctg gaaatgcttc
     1801 gatatctgtt ctccccttga ggactgcacc ttcttcaaag ataagatgac tcttatcttt
     1861 taaaaaaagg gctccgacta aataaatgcc ttttggaata aatacaggta agtgttcttt
     1921 cgcaccttga tcgattgctt tttgcagtat tattgtatca tcttgttgag tgtcgtttgt
     1981 tttaaagttc aaagcattga taatcccttt ttttgtccgc aatacgtttc tccctatctt
     2041 tcattgattt ttttcaattc ttcaccaata aatacagttt ttgaacgatt cagtgggtaa
     2101 cctactagca aaatgattgc ggatacaaat aagcagaatg ctggtaacaa gtttgccatc
     2161 gtataaatcc tttcttgtac taaaactgat tggatggctc cacctgttgt agacgattgg
     2221 taaccaatga gtgccagcat aaatccgcct accgctcctg cacatgcttg tcctactttt
     2281 ctcgcaaaag aatttactcc atatactgtt ccatcttctc ttaagcctgt cttatattga
     2341 tgataatcaa tcacgtcagt aatgaatgcc cagaccatca aattgaataa accagatcct
     2401 aaagtagcga taaataaaag aatcagatat acccatgcat ttgttatatg aaggaaatac
     2461 atcagcaagt acatacatga agaagcaagt aacgcaaata cgcttgcttc ctttttacca
     2521 aataattttg tcagctttgt agcaaatggt gctaaaacta ctactgttcc gtaagtaaat
     2581 aaaagagcaa cagacattgc ttgtttatta tggaagtaat cattgaataa ataagtcata
     2641 ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt caacaatgac taaaataatc
     2701 agtgctcgat tagctaacaa accttttacc aactgagaag cagatacttt ttcgtttttg
     2761 acaactctta ctctttcagt agtcaattta caagttaaca tatatgcgac aaatgctatt
     2821 gcagcacatc cgattgaaat ccaaaagaaa tgattccctg aaacgacttg ttgtccactc
     2881 ttgttatggg tatacattaa aatcggaatg aagaatcctg tagttgatcc gccaatcgca
     2941 gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat taggatttga gctaattgct
     3001 gctgccattg ctccatacgg gatattgatt gttgagagaa aaatcccata aacaatgtat
     3061 gaaacaaaaa tataagcaat cttcaccgtc attggaagat gttggacgaa tggcaaaaac
     3121 aaaatgacag tcactaagca aaatggatac ttcatttttc tgatccaagg aataaatcgg
     3181 ccagtttctg ttaactctga gacatcacat aatcttccaa ccatcacatc agcaaacgca
     3241 tccaaaattc tcgaaatcaa gaataacgta cctacgattg cccctgaaac gccaagaaca
     3301 ttcgtataat aaatcatcaa gaaactattg actaaaccta aaataaaaca gttacctaga
     3361 tcaccgaaca tgtagcctat cttatcggaa atcccaaatt cctttttttc tttttttact
     3421 gtatttgctg taacagcatt ttgtgaaagc atttgaattc ctcctacctc ttctagtaaa
     3481 cgttttcaaa attataactc tttttttctt gtttttatat tcacaatgtt ggctttttat
     3541 tgcattatct tgacagataa aaaacaaaaa at
//
LOCUS       DAPCMU010000196         3567 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPCMU010000196 DAPCMU010000000
VERSION     DAPCMU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.202"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            61..1182
                     /locus_tag="RD744_002639"
     CDS             61..1182
                     /locus_tag="RD744_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDU0558601.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1440..2942
                     /gene="eat(A)"
                     /locus_tag="RD744_002640"
     CDS             1440..2942
                     /gene="eat(A)"
                     /locus_tag="RD744_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDU0558602.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2970..3536
                     /locus_tag="RD744_002641"
     CDS             2970..3536
                     /locus_tag="RD744_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0558603.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1218 a          612 c          801 g          936 t
ORIGIN      
        1 tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg agggatacag
       61 atggatattt tggcggcaat tgattcattt aaaggaagtg cttcttcatt tgaactaaat
      121 caggcagttc taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca
      181 gatggcggag aaaatactgc agaagcaatc tattacgcgc tcaggggaac atggcgtacc
      241 attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt gacaacttgg
      301 caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat
      361 aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga acttattaaa
      421 gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc
      481 gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga
      541 aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa
      601 gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct
      661 tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa
      721 gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca
      781 ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt
      841 tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat
      901 gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac
      961 ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga
     1021 gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt
     1081 ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta
     1141 ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct
     1201 ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa
     1261 gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag
     1321 attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt
     1381 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa
     1441 tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat
     1501 ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg
     1561 gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag
     1621 taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa
     1681 cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc
     1741 aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag
     1801 agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg
     1861 atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa
     1921 agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg
     1981 accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct
     2041 atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa
     2101 aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag
     2161 aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata
     2221 aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc
     2281 ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt
     2341 ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc
     2401 aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc
     2461 gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg
     2521 gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt
     2581 atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc
     2641 aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt
     2701 ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat
     2761 ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct
     2821 tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg
     2881 aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct
     2941 aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag
     3001 agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag
     3061 tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag
     3121 cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac
     3181 gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg
     3241 aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact
     3301 ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg
     3361 tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc
     3421 tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta
     3481 atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat
     3541 gtttttgtgt tatttaaata aaatatt
//
LOCUS       DAPCMU010000197         3465 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPCMU010000197 DAPCMU010000000
VERSION     DAPCMU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.203"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..2232)
                     /locus_tag="RD744_002642"
     CDS             complement(<1..2232)
                     /locus_tag="RD744_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558604.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="RD744_002643"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="RD744_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          972 a          768 c          548 g         1177 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtc
//
LOCUS       DAPCMU010000198         3200 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPCMU010000198 DAPCMU010000000
VERSION     DAPCMU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.204"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..665)
                     /locus_tag="RD744_002644"
     CDS             complement(<1..665)
                     /locus_tag="RD744_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558605.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFS"
     gene            1318..1413
                     /locus_tag="RD744_002645"
     CDS             1318..1413
                     /locus_tag="RD744_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDU0558606.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1715..1960)
                     /locus_tag="RD744_002646"
     CDS             complement(1715..1960)
                     /locus_tag="RD744_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558607.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            2305..2589
                     /locus_tag="RD744_002647"
     CDS             2305..2589
                     /locus_tag="RD744_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558608.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2592..3008
                     /locus_tag="RD744_002648"
     CDS             2592..3008
                     /locus_tag="RD744_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558609.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT         1065 a          597 c          500 g         1038 t
ORIGIN      
        1 gaaaaagtaa attctaattt ggcgatttta ttgccctttt ttgcttttat ttttgtgact
       61 ttaagatcat tgaagtattt ttttagttct cgtaatgcag gatttaatac tttttgatca
      121 atgttaccca tttgatagta ttctgggata tccattaagc tcctaaactc ttcaatacga
      181 actacataat aaccagtaga acggtactgc ataagcagtc ggaatagagt ttttgcatac
      241 gtagaacgca acgacgtgaa agcagataat tcaaatctac taaattcagc tgttaaacca
      301 ttaataatat attctaggtc cggattaaca ctgacttcaa caaacttttt tgttttattg
      361 atttcaaaac cagtaaacaa aacaaagtag cgaatagaat cctcatcttc agtacggtaa
      421 gtaagattaa gcatatcttt atagagcttt tctaaatcac cgacaaaggc ttttgtagca
      481 gtcatcttat aatcacttaa ctcttttaat tcctcgaaag aaaatctaac ttttttcaaa
      541 ccacgatctt tcatacgagc gcataatgca aaaaataaat ccatctgctt agcattgaaa
      601 tttttcagcg gtactaaatt caattcattt tgatatttga ctgttaattc cgttttttca
      661 tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca actcgtaaat
      721 aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg ttagtactca
      781 gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag
      841 tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag ggttctaaaa
      901 aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc cgccaaaaaa
      961 tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa tctagctccc
     1021 tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc agcagtcgcc
     1081 tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc ctgagagctc
     1141 gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct aaagcgcctt
     1201 acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag gcttattttt
     1261 gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg gaaggaggtg
     1321 tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag cgctttgttc
     1381 agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac tcagcccaca
     1441 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg ttcacttatt
     1501 tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca aaagtctaaa
     1561 atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag agctcgtatt
     1621 tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa gctaaaaaac
     1681 tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca tgaaattcgc
     1741 tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt tgcattaatt
     1801 tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat ttttcgtctg
     1861 ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt tgaaaatatt
     1921 cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta gaaataaaat
     1981 cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa ataaattttt
     2041 gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc ccgaatttta
     2101 aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag gctgtgccta
     2161 tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta gcttatgtat
     2221 agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac ataaattata
     2281 cataattgca aaaggagatt ttttatggac attttagttc ggaatattga tccaaagttt
     2341 gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga aaaatggagt
     2401 cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact tacgacttac
     2461 aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac agaaaatact
     2521 caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct tatcggagga
     2581 atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac cctagtcatg
     2641 ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac tcggaagcca
     2701 ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc agcaatcagg
     2761 caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc gcagaatttg
     2821 ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa gtatatcaag
     2881 aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga ccaaagaatc
     2941 atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca aattttctaa
     3001 aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag ccccatacaa
     3061 tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac ttgcttaatg
     3121 taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag aaatctttgg
     3181 caaatctgac gttaggcttg
//
LOCUS       DAPCMU010000199         3128 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPCMU010000199 DAPCMU010000000
VERSION     DAPCMU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.206"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            103..1347
                     /locus_tag="RD744_002649"
                     /pseudo
     CDS             103..1347
                     /locus_tag="RD744_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="RD744_002650"
     CDS             1360..1992
                     /locus_tag="RD744_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0558610.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="RD744_002651"
     CDS             2197..2424
                     /locus_tag="RD744_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDU0558611.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="RD744_002652"
     CDS             2493..2759
                     /locus_tag="RD744_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDU0558612.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="RD744_002653"
     CDS             2749..3018
                     /locus_tag="RD744_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDU0558613.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAPCMU010000200         3110 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPCMU010000200 DAPCMU010000000
VERSION     DAPCMU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.207"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..171
                     /locus_tag="RD744_002654"
     CDS             <1..171
                     /locus_tag="RD744_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HDU0558614.1"
                     /translation="DNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL
                     PAMPDLVDKRRS"
     gene            386..1354
                     /locus_tag="RD744_002655"
     CDS             386..1354
                     /locus_tag="RD744_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDU0558615.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1347..2378
                     /gene="vanA"
                     /locus_tag="RD744_002656"
     CDS             1347..2378
                     /gene="vanA"
                     /locus_tag="RD744_002656"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDU0558616.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2384..2992
                     /locus_tag="RD744_002657"
     CDS             2384..2992
                     /locus_tag="RD744_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDU0558617.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          919 a          589 c          767 g          835 t
ORIGIN      
        1 gacaatgctc gttcttccga tacgggtggc gcgggacttg gattggcgat tgcaaaagaa
       61 attattgttc agcatggagg gcagatttac gcggaaagca atgataacta tacgacgttt
      121 agggtagagc ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat
      181 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
      241 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
      301 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
      361 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
      421 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
      481 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
      541 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
      601 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
      661 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
      721 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
      781 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
      841 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
      901 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
      961 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
     1021 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
     1081 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
     1141 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     1201 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     1261 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     1321 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     1381 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     1441 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     1501 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     1561 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     1621 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     1681 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     1741 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     1801 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     1861 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     1921 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     1981 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg
     2041 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     2101 gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga
     2161 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     2221 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     2281 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     2341 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     2401 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     2461 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     2521 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     2581 atgggacggt taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc
     2641 ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc
     2701 aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct
     2761 ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga
     2821 acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt
     2881 gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt
     2941 attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa
     3001 ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga cgtatgtaac
     3061 tggttcttag ggaatttata tatagtagat agtattgaag atgtaaggca
//
LOCUS       DAPCMU010000201         3090 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPCMU010000201 DAPCMU010000000
VERSION     DAPCMU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.208"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(582..1538)
                     /locus_tag="RD744_002658"
     CDS             complement(582..1538)
                     /locus_tag="RD744_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558618.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1647..1892)
                     /locus_tag="RD744_002659"
                     /pseudo
     CDS             complement(1647..1892)
                     /locus_tag="RD744_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2037..2459)
                     /locus_tag="RD744_002660"
     CDS             complement(2037..2459)
                     /locus_tag="RD744_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558619.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2639..3010)
                     /locus_tag="RD744_002661"
     CDS             complement(2639..3010)
                     /locus_tag="RD744_002661"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDU0558620.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT          921 a          684 c          517 g          968 t
ORIGIN      
        1 gaatgctgat ttacctatgt tttacgtaat ggtctttagt tatttttgct cataaaatgc
       61 ataaaacacg cgtttttatg tgtgcttgaa atttcttggc tctataattt ctaagactga
      121 taaaaatatt ctatcggttc tttttgcaaa taaaaaaaca tatagaagct ccattagatt
      181 gaatcaccac aacacaatca aaggagcatt ccgtatgctt gattttcatt ctataaaact
      241 acttttagga atcaaagaaa atcacaacgg ttcaaactaa aagcaagaat atccgaagta
      301 ccattgtttc atcgatcatg agaaaagcat tcacctctgt ctgcagatgt tcttatatcg
      361 ctctgtcagt cctattaata aagtactgtt ttgttttcaa aaaaactatt acgagtactt
      421 tttaagagaa tgccttgcaa atattcatca cacaccgtta tataagtcaa atagtttctt
      481 tcagtcgtca agaaaagatt ctcctgtcct tggcattatc agttaagtag caacttaatt
      541 ctcggacttg ttctactcta gcaaaattta ttttcatcaa cttacgtcca aatgtttttt
      601 tgaataatta cttcatcgcg ggtctggaag acttgcctca ttcgataagt cactagcttt
      661 tggcgtcctt caggatttct agtatacacc aactgacatt tgggcagcga cgattgattt
      721 aattcgttta gaaaaaggtt agcgatttgc ttatttcggc caaagcgttc tggtaccgtc
      781 agaactatct ttttcccttc gattaaatac cgcggattct ccagcggtgc taaaagctcc
      841 ctcatagctt cagtaaaaag ctgtccttct cgatagccag cattctcaag atagcatcta
      901 aattcctgct gatcagccgc tacgattact tttctatcac taacttcacc aaggcgctgc
      961 aacgtcgttt gaacgcatat ggccaagctt tttttgcggg cacattcctt ttcccatagc
     1021 ttttgtcgcc tacgaatttg gaaatattcc agcaagttac ctttaaatat agctgaccag
     1081 aagcttagag gctctatatt ttttactact atcggcaatg aagtttcttg tttttgcttt
     1141 acctgatcga ctttcctaac gacgattggc atagataggt gccagccatc agctaaagca
     1201 gtactccatt gttctttcaa ccgacttcgt tcacaagaaa aatagaacat cgactgagta
     1261 taagtttcca tcgtggtggt cgtatattcc agtggtaaag caaagcgatc gccaccactc
     1321 tcaatttttg gtgaagattc aaaggtcaga cctgaaatat tgcccaaacg ccgtagcatt
     1381 cttttcaaat caggctgttc agacagagga actcccggta atccaatcgc taaatgccaa
     1441 atgttcgtta ctttattttg tgcacctagg cgtaagcccc gccctcgtag ttgttgtgtt
     1501 tgcacaaaag tggaagcttg attagccaag atcaacagat taattgcagg acaatcccag
     1561 ccttctccta gcaaactaac ggttcctacc aaaatattca acttcttttc ggttaatagg
     1621 cgagtaactg cttgcatcaa tgcttggcgg tttttgtcat ccaccgtcaa ataaaaataa
     1681 cccgacatat aaggatgttc attcataccc gtcgctagtt ctgccagctc aacagttact
     1741 agcattttcg gtaacagtaa gaactctcca cagatggcac cgatttgctg agtgttacct
     1801 aatagacgaa caacttcatc aaacaaagga accacgccat aagctaaggt accatcactg
     1861 cctgctaata cctccttgca aatacgatcc atcaaaatga ccgctgtcag ttcttgcttc
     1921 agctgaactt cttcttttaa caaaatttga caaactgctt gctttttctc aggaatcgcc
     1981 tgactaccaa cagtgtctgg atactctgga aacaaatgca gcgactcatc aaacaatcat
     2041 ccagcttttt tcaattgata aaaaataggc aattccggtt gatcttcaaa catctgctga
     2101 taaatagtga tcagctcttt tttacctaaa gatggaactg aagctttttt accttgctgc
     2161 ttagaactgc ctaatagctg ccaatgttcg ggtatcaatt catagttatg atgattcaag
     2221 taaattaatg cactagtgaa cagctcaaaa ttttcataaa tttccttcga atgctcaagc
     2281 ggatgctgaa caaattctaa agaaagcaaa taatccaccg tctcttggct ttccattaac
     2341 gcttccacca gctcattttg tttctctaaa aaggtaaaat aatgctgagt agtttcctga
     2401 ttagccggaa tcaaatatag aaaatcctga tgaggacata gaacctcctc ctttaccaac
     2461 tcgggaatgc taatttcttc gtcaatcagg ccattttaac tcaatatatt gcttccactc
     2521 cgtctgagag ctatcaatcg gaggcgtggc tgtcaaggaa atcgtcaaca gattcggttg
     2581 ttttttcttc aagtcaatca acaattcacc ccagcttttt tttaaatgat gagcttcatc
     2641 aaaaaactaa cagctccagc tgatcaccat aagttggctc ctccttcagt cggttgtaaa
     2701 tggtctgata cgtttccact gtaatatgac taggatgttc aatcgcccca gaaagatagc
     2761 aactatctgc tggatttact aagaaatatt cctcgattgt tgttaaccac tgtttcttca
     2821 atattaggga aggaaccaaa attaaggttt tacaatccaa acgtcggatc atctcaattc
     2881 ctaaaaaggt cttgccagcc cctggtggag ccactagatg gactgaattg ttatttaaat
     2941 aagtagcaaa gttagtcaat atacgcttct gatagctacg ccactgaatt ttttcatcag
     3001 atagccacat cccaggtccc cctctcacgt attttctcta tcataatcta aatgaataca
     3061 gcaaaaaaaa gatttcttca agaatatgtg
//
LOCUS       DAPCMU010000202         3081 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPCMU010000202 DAPCMU010000000
VERSION     DAPCMU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.209"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(298..912)
                     /locus_tag="RD744_002662"
     CDS             complement(298..912)
                     /locus_tag="RD744_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0558621.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1518..1895)
                     /locus_tag="RD744_002663"
     CDS             complement(1518..1895)
                     /locus_tag="RD744_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558622.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2195..3076)
                     /gene="galU"
                     /locus_tag="RD744_002664"
     CDS             complement(2195..3076)
                     /gene="galU"
                     /locus_tag="RD744_002664"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDU0558623.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          933 a          644 c          475 g         1029 t
ORIGIN      
        1 tattaagaga caagaaagaa tccatatgga ttctttcttg tctctttttc cactactttt
       61 tatttttctg acactacata ttcttataac tcatttctca taactgtgaa acaacttata
      121 aaaataacaa actttaaaca aaacaagctt aaaatcaaag atgatccatc ttaaaataac
      181 taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa tcgttttctt tttttcaaac
      241 gctccatttt tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta
      301 ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa
      361 aaagatcgtt gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa
      421 gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg
      481 caaaccactg ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat
      541 taatggatga ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc
      601 ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc
      661 ctgtcctgca ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact
      721 tggtacaaga tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct
      781 agcatatatc tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg
      841 aagcatcagc ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata
      901 acataaactc atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct
      961 aatctaagga agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt
     1021 ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac cttgacatag caaataaaac
     1081 aacgtgcaga ccatggaaaa tatctacata ggggtagaat tttcacccta gattaggtac
     1141 aataaattag gtcgatgcac tgcattgact atggcaaaat gatcggtcag ggtgtatgtg
     1201 tgtgactggt cattccctga ttcttagcgc caactaaggg tcagggagcc tttttttatt
     1261 cagctgtttc tatctgaatt tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat
     1321 acaaaaaaac attggtatga tttatatata tataaataac ataaaaaaag gaatggctta
     1381 tcccgataca acaagcattc ctttaaaaaa gattctttta tataagtgaa aaaagctttt
     1441 ttccaccaat taaataaatc tctcatctca atagtttatc cattatttta tctttttttg
     1501 cgtataccta ctaaatatta ccgtcttctt ctcgttcttc tactttgttc gagagaatct
     1561 gtactagaaa aagtagttgt atgtctacct tttctgccta tccgagactt tcttttcata
     1621 tatgggatat aaatgattag atacagtaca gcaaaaaata aagcaaatac gcaagcttcc
     1681 caactaccta acgccaattc aaccatcaag gtagcaaccc caacggatgc tgcataccag
     1741 ataaaaatca gaaacgtttc tttttttctg accatgcgaa acatgataaa tagactaata
     1801 agcaatagtg gcaacacaaa tatccaattg cttccttcca tcagccactt gagtatatca
     1861 agcagcgact gtgcaacagc gactaatttt cccatagtga acacacacct ttttttataa
     1921 ttaaaaaatt cgccttttta ttctactatg aatcttctgg cttggctttc atttctctta
     1981 tgtaatcatt ttcttttata caacttaaaa aaaaattaaa ccattagcat aatattttct
     2041 gaatctattc acgaaagtct ttttctatct ttcgttttcc acgactcaac tgatctagag
     2101 tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat
     2161 agcctttcat tctcagcagg ccacttattt ccagttaact ttctgttctt agctttttac
     2221 tcaactggat aatatatgca cttaatgaag tcccaatttc cgaatgttga agtccatatt
     2281 caatatttgt cttcatatat cctaacttat cgcctacgtc gtatcttttt cctttaaact
     2341 ctctcgcata gaccgcctct gtctggttaa gagtctgaag tgcatccgtt agctgtatct
     2401 cgtttcctgc tcctggttct tgagtttcta atatctcaaa tattttagga gtcagtagat
     2461 aacgtccgat aatggctaaa ttacttggtg cttttcccat ttcaggtttt tctacaaacc
     2521 cttttactct gcaacgaccc ttttcgctat ctgttttagg atcaataatt ccgtatttag
     2581 atgtttcatt ttctggtaca cgcatgacgg caacagcgga agcttgtgtt tcttcataat
     2641 ccatcatcaa ttgttgagtc aatgggatat catctttcat caaatcatcc cccagcatga
     2701 cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag cacggcatcc cccagtcctc
     2761 ttggatgaga ttgacggatg aagtgaagat tgacttgagt cgtttcttca actaaagcga
     2821 gtaattctgt tttttccttt tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt
     2881 gatcttcaat gggtcttttt tcttttcccg ttacgatcaa gatgtcttcg attcccgaat
     2941 ccaatgcttc ttccacaata aactggatcg ttggtttatc cacgattgga agcatttctt
     3001 ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag cccagcggca ggaatgactg
     3061 cttttttgac cttcacttgc t
//
LOCUS       DAPCMU010000203         3070 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPCMU010000203 DAPCMU010000000
VERSION     DAPCMU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.210"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            40..795
                     /gene="fabI"
                     /locus_tag="RD744_002665"
     CDS             40..795
                     /gene="fabI"
                     /locus_tag="RD744_002665"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDU0558624.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(905..1918)
                     /locus_tag="RD744_002666"
     CDS             complement(905..1918)
                     /locus_tag="RD744_002666"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDU0558625.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2083..3003
                     /locus_tag="RD744_002667"
     CDS             2083..3003
                     /locus_tag="RD744_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDU0558626.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          894 a          621 c          589 g          966 t
ORIGIN      
        1 cgactatcaa atttttatca gaaacaaagg agagtcatta tgtcatttct agaaggaaaa
       61 aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg cttggggttg tgctgaagca
      121 atgttgaaac aaggggcaga aattatctat acttaccaaa atgaacgaat gaaaaaatca
      181 ttagtcaaat tggtcggcga agaagcattc actgtagaat gtgatgtttc tagcgacgaa
      241 agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg gggagatcca cggccttgtc
      301 catgcagttg cttacgccaa caaagaagaa ttgtccggaa atgtgtctga tatttccaga
      361 gacggctatc aattagctca agatatcagc agctactctt taattgctgt atcaaaatac
      421 gcacaaccgt ttcttgcacc aaacagcggg atcgttagca tgacttatct aggttcagaa
      481 cgcgcaatcc cgaattataa catgatgggt attgcgaaag cttctcttga agctgctatc
      541 cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat ctcagctggg
      601 gcaatcaaaa cattagctgt cacaggtgtt aaagactatc aaaaactgat ccagttatct
      661 gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa tgcttgtgct
      721 ttcttaatca gcccactttc cagcggaatc atcggtgata ttatctacgt tgacaaaggt
      781 gtccatcttt cataaacttt tgttgcattc tttaaagtaa aacatataaa aatcaggaaa
      841 aaacttttta aagtctcttc ctgatttttt ctttttacgt ttttttcaac tcattacttt
      901 gaaattattc cacgacttca atccagcctt ctggtgcttc tatgtctcca aattgaatac
      961 ctgtcaattc atcatacaac cgttttgtta ctggtcctac ttccgtttca ctataaaata
     1021 catgaagatt gttattgtga taaatcccac cgattggaga aataacagcc gctgtaccac
     1081 atgcaccagc ttctgcaaat tgatctagat catcaatgaa aatatctcct tcaattgctt
     1141 gcagtcctag tcgttcttca gctaactgca acaaggaata tttcgtgata cttggtaaga
     1201 tagagggcga ttgcggagtg atgaattttc cgtcttttgt aattccaaag aaatttgctg
     1261 cacctacttc ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa tctgagtaat
     1321 ccagttcttt tgcttctgtt cctggaagta aactcgctgc gtaatttccg ccgacttttg
     1381 ctgcaccagt ccccttagga gcagcacgat cgtaatcaga tacaataaaa ttcgttggtt
     1441 ttagtccacc tttgaaatac gctcctactg gcgtacagaa gacgacaaat aaatattctg
     1501 gagctggatg gacaccaatg ttttcaccga ccccaatcag taaagggcgc aagtataatg
     1561 tagcgcctgt tccatagggc ggcacaaaat cttcatttgc ttttaccact tgcttgacag
     1621 catcaacgaa catcttttca gggaccattg gcatcagcaa tcgtctagca ctttgattca
     1681 tccgtcgact attttgttca ggtcgaaaga gattgacctt gccgtctttt cgacgataag
     1741 cttttagacc ttcaaaacat tgctgaccat aatgaagcgc tggagagcct tcatgaatcg
     1801 tcaggtaatt atcttctgtc aattctcctt cttcccattg tccgtctttc catttggcaa
     1861 taaaacgcca aggagttttg atataagaaa aacctagatt gttccaatcg atttgcatat
     1921 acttaccacc ttcgttattt tctccacatt ttatcattat ccatattatt tgtcatttta
     1981 ttttcagctt ttttccttca ttatagttat ttttgctatt tccttttttg aaaacttctt
     2041 ggtacaatac agaaagaatc aaattttaga aaagaggatt ttatgttttc attattagca
     2101 actgccggta cgacagactc ttctggcgag acattgacag atcaagctgt cagcaaagta
     2161 aatgcgtttc agcggttttg ggacgggatt gattgggacg ccattttggc tgcctttatc
     2221 caaaaaagtt tatatctgat ttttttgatc gtcctttttt ggattcttca ccgtgtggga
     2281 aaatatctga ttgataagtc ttttaatcag tattctaaaa aagccattct aaacgagagc
     2341 cgcttaaaga ctttacatag tcttttaaat actatttttc attataccct tggtttcttt
     2401 tttatctatg caattttatc tgctatcggt gtgccgatcg gatcactttt ggctggtgcc
     2461 ggaattgcag gtgtggcaat cggtcttggt gcccaagggt tcatgagtga tgttattacc
     2521 ggatttttta ttatcatgga acaacagatg gatgttgggg actacatcaa gctagcaaac
     2581 ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta cccttcagct gaaagcaacg
     2641 gatggtactg tccatttcat cccgaatcga aacatcacca cgatcagcaa tctctctcgt
     2701 gcaaatatgc aagtactttt agatattcgg attgttcctg aagaaggata tgacaaaatc
     2761 tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga agagctccaa
     2821 actgaacctt ctatctttgg attagtggat cttggaaaca gcaattttgc gattcgtacg
     2881 gtctgctacg cattgaacgg aaaacaatat gccttaaaag aagagttctt gagctcctat
     2941 gtcaaagaat tgacagcttc tggtttcacg attccgaaca gtccaattgc tgtcggaaaa
     3001 taatttggac aaacgatgca acgaatctca ctaaaaagag cgtgggacaa aagtaaagaa
     3061 cacttttgtc
//
LOCUS       DAPCMU010000204         3013 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPCMU010000204 DAPCMU010000000
VERSION     DAPCMU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.211"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            177..359
                     /locus_tag="RD744_002668"
     CDS             177..359
                     /locus_tag="RD744_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDU0558627.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="RD744_002669"
     CDS             462..746
                     /locus_tag="RD744_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558628.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="RD744_002670"
     CDS             762..998
                     /locus_tag="RD744_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558629.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="RD744_002671"
     CDS             1020..1274
                     /locus_tag="RD744_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558630.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="RD744_002672"
     CDS             1356..1541
                     /locus_tag="RD744_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDU0558631.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="RD744_002673"
     CDS             1608..2003
                     /locus_tag="RD744_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDU0558632.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="RD744_002674"
     CDS             1993..2370
                     /locus_tag="RD744_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558633.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="RD744_002675"
     CDS             2392..2766
                     /locus_tag="RD744_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558634.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DAPCMU010000205         3001 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPCMU010000205 DAPCMU010000000
VERSION     DAPCMU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.212"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(37..1257)
                     /locus_tag="RD744_002676"
     CDS             complement(37..1257)
                     /locus_tag="RD744_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDU0558635.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="RD744_002677"
     CDS             complement(1277..1888)
                     /locus_tag="RD744_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDU0558636.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="RD744_002678"
     CDS             complement(1931..2863)
                     /locus_tag="RD744_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDU0558637.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          824 a          623 c          518 g         1036 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 a
//
LOCUS       DAPCMU010000206         2941 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPCMU010000206 DAPCMU010000000
VERSION     DAPCMU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.213"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(91..633)
                     /locus_tag="RD744_002679"
     CDS             complement(91..633)
                     /locus_tag="RD744_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDU0558638.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            768..1157
                     /locus_tag="RD744_002680"
     CDS             768..1157
                     /locus_tag="RD744_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558639.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1161..1925
                     /locus_tag="RD744_002681"
     CDS             1161..1925
                     /locus_tag="RD744_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0558640.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2054..>2941
                     /locus_tag="RD744_002682"
     CDS             2054..>2941
                     /locus_tag="RD744_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDU0558641.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKG"
BASE COUNT          946 a          492 c          603 g          900 t
ORIGIN      
        1 atgtcgatgt tttttaatca aaataataaa taaaaactgt ctaacattta aaatttaatt
       61 tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc tcactttttt
      121 agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag cacgctcttc
      181 gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa gtatttcgag
      241 aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat aaccgtgttc
      301 tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa tcgttgtgac
      361 catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga tacctactgc
      421 gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta aaacaaggga
      481 taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa tcagtcgata
      541 gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa aattaatgag
      601 gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca tttatataac
      661 aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat acaaaaaaat
      721 gataaaatga ttataataga attataaaga taggttaggt gattaaaatg aaaacggtca
      781 atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct cagtatatac ttaggtgtct
      841 attcgattca acagccaatt gaggtagaga acattgtttt tcagatgtcg ctgacagatg
      901 gtttaatttt aagcgttttt ttctttttga tactgtttat tggtaatatt ttcggtagcg
      961 ttgccagttt tttgaatttt tctatttatc cactcctttc gctagcatta ggagtggtgg
     1021 gactttgttc tctgtgctta tttcccgagc cattcagtcc tgcatttata ttatttggta
     1081 cactgaacct gttccagaca actgttggag catggttgct atggcgatca ggaaatttaa
     1141 tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa acgttctgtc catcatattc
     1201 cagtgttaga agttgttctg aaagaattaa gagaggaacg tattcccgtt gtcatctatt
     1261 atcacggatg gcagacaagt aaggagttag ttttgacgca aggaagaaaa attgcgcagc
     1321 agggcattcg tgtgatcctg ccagatgcga tgaatcatgg ggagcgcaaa cagccggttt
     1381 cttctattcc atcctataca ttttgggata gtatctacgg aaatctgttt gaatttgaca
     1441 cacttatcaa gcatttagaa aaaagaggtc ttctacatga tcagcttgca gttggcgggg
     1501 tttcgatggg gggaatgaca acatgcgcct tgttggctaa acatcctcat atagtagcgg
     1561 gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg aaaagaaatc gtaaggcgcg
     1621 catcggaata taattatgtc gtaccaaaag actattttga tttgatttca tgggttaaga
     1681 gctatgattt gtcattgcag ccggaaaagt tagctggacg tcctctgttc ttttggcatg
     1741 gacaagctga cgaaaaaatc ccttatatac aaacggaacg atttgtagaa aaaaatcggc
     1801 ataagtctta cggaaaagaa attgtttttc gttcctcaaa aacacagcga catatggtac
     1861 aaataccact aatggaagaa gcttccactt ttttagcaaa aaatttgata aaaaagtata
     1921 aataattgaa acggaaaatc actcatgacg atagaggatt ttccgttttt tattttgggc
     1981 tataataaac tctgttagga aaatttggta taattgatag taagaatgac aacatgttag
     2041 aaaggaagag agcatgccca ctttagcaga tgttgcaaaa cgtgccaatg tttcaaaaat
     2101 gacggtctct cgagtcatca atcatccgga acaggtgact gacgaattga aagagttagt
     2161 tttctcagca atggctgaac tggattaccg tccaaatatt gctgcaaaag cacttgtcac
     2221 gaatcggtcg cagatcatca aattatttat actggaagaa atcgacacaa ctgagcccta
     2281 ttacatgaat ttgctgatgg gaatcgctaa acggatcggt aaagcgcatt attctctcca
     2341 attagttaca aatgatgcct tcgacgtcgg ttcttgtgat ggatacatca tcacaggtgt
     2401 cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa ccagttgtct tgtttgggga
     2461 aaattctgaa ggatatgatt atgtagacag cgataatgaa tatggtacag caaaagcaac
     2521 gcaatatgcc ttagaaagag attatcaaac gattatttat atcggtatcg aagtggacga
     2581 gccttttgaa atttcaaggg aaactggtta tttatctgtg atgaaggaag ttgatcttcc
     2641 tagtaaaatc ttgcggtttc caaatcattc gacagaagta gaaagatata tgacagaaaa
     2701 ctgggcagat tttacagata aaacttgttt tgtatgtagt tcagatcggc tagcactagg
     2761 agcagtaaga gggattataa atgctggagg acacttgccc gaagaatttg gtgtgattgg
     2821 atttgatggg gtatttttag accaaatcag ttcacctaaa ttgacaacga tcaagcaaga
     2881 tattatacgt atgggtgaag tatgtggaga aatgttgtta aaaaaaatag aagaaaaagg
     2941 g
//
LOCUS       DAPCMU010000207         2923 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPCMU010000207 DAPCMU010000000
VERSION     DAPCMU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.214"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            107..2167
                     /locus_tag="RD744_002683"
     CDS             107..2167
                     /locus_tag="RD744_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDU0558642.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2250..2819
                     /locus_tag="RD744_002684"
     CDS             2250..2819
                     /locus_tag="RD744_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558643.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1044 a          529 c          563 g          787 t
ORIGIN      
        1 tactctccta atagtccatc ttcttttgat tttatttata taatgaaatc atcttatgaa
       61 aattactact tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag
      121 acgttcaaaa caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga
      181 aaaacactac gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta
      241 tatgcatgta cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc
      301 tgagaccact gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca
      361 gctacttcct gcattgactt cctataataa agtcagttat cctccaacga taaaccacta
      421 ctggttaatt cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc
      481 tatcttttcc gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat
      541 tttgaatcat gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata
      601 tgaaaaagat tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt
      661 ttcgatatct gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc
      721 caaagtaatt tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat
      781 ttccttgtcc tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca
      841 tgttcgtgct gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag
      901 ttacgcccct aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa
      961 agaagaaatc aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt
     1021 attaacttat atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt
     1081 agaagcatct gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa
     1141 agaacttcaa gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc
     1201 tgctgaattt tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa
     1261 agtcacttct atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc
     1321 ggagatcctt gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga
     1381 aattaccttt acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat
     1441 tgttcatcca aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt
     1501 aggtaaaccc gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga
     1561 tggttatttg accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta
     1621 ttcactccaa gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat
     1681 agatcaaaaa gaacaaaaaa gacgagaaga aagcttgtca gaaaaaaaac aacaaatggc
     1741 tcatgcagtt tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat
     1801 atctagaaaa atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca
     1861 agtttctgga ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga
     1921 atttgttgct aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa
     1981 aaaacgcttg tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc
     2041 tagtccggga acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat
     2101 gcgaggtatg gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg
     2161 gttctgattt atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg
     2221 gcataatgga gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa
     2281 tctggatgac aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg
     2341 aatttcgatt ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg
     2401 gggaatttca aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc
     2461 ctgaagaact tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa
     2521 cctgtaccgc ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag
     2581 cagtgaaacc agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc
     2641 aagaatttcc taccctccat acggacggac caatctatct tcacacacag acatggagcc
     2701 atcaggcgat tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg
     2761 gcacacctaa tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac
     2821 gaaatggtga aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc
     2881 ttactttttt gagttaatac tatcggataa ctaatggcat gcc
//
LOCUS       DAPCMU010000208         2820 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPCMU010000208 DAPCMU010000000
VERSION     DAPCMU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.216"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            95..1054
                     /locus_tag="RD744_002685"
     CDS             95..1054
                     /locus_tag="RD744_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HDU0558644.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="RD744_002686"
     CDS             complement(1487..2059)
                     /locus_tag="RD744_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558645.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="RD744_002687"
     CDS             complement(2075..2680)
                     /locus_tag="RD744_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDU0558646.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          468 g          885 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
//
LOCUS       DAPCMU010000209         2802 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPCMU010000209 DAPCMU010000000
VERSION     DAPCMU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.217"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            274..2169
                     /gene="asnB"
                     /locus_tag="RD744_002688"
     CDS             274..2169
                     /gene="asnB"
                     /locus_tag="RD744_002688"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDU0558647.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(2364..>2802)
                     /locus_tag="RD744_002689"
     CDS             complement(2364..>2802)
                     /locus_tag="RD744_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDU0558648.1"
                     /translation="NMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIAIDQ
                     AKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKSKGA
                     KKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT          913 a          478 c          531 g          880 t
ORIGIN      
        1 acacgatctt taggtcgtgt ctttttttgc tggttttttt ggaaatagca tccactaata
       61 tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat
      121 tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt
      181 gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata
      241 aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac
      301 gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga
      361 cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag aagattaagt
      421 attattgact tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt
      481 atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc
      541 cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga
      601 acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag
      661 aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg
      721 aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac
      781 aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac
      841 ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa
      901 gatggaaaaa tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat
      961 tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat
     1021 acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta
     1081 acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat
     1141 gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg
     1201 atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct
     1261 gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagtgtg
     1321 cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga
     1381 ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa
     1441 ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaacttcca tggacggatc
     1501 catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc
     1561 gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc atctgtggat
     1621 gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt caataagatg
     1681 caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc agataaactt
     1741 tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg
     1801 gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga tattttcaga
     1861 caagctgcga acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct
     1921 gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc
     1981 gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac
     2041 catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca
     2101 tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg
     2161 actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg tttaacactg
     2221 agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttatg
     2281 gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg acacgatttt
     2341 cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt cccatctttt
     2401 ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt ttcgattgct
     2461 tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt cacgatccat
     2521 tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc acgaggattc
     2581 gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg tttttctgat
     2641 tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat cgcacggtta
     2701 tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt tcttgtcaat
     2761 ttatctaagt ttttcattga tgcaggaaag tccttcatgt tc
//
LOCUS       DAPCMU010000210         2796 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPCMU010000210 DAPCMU010000000
VERSION     DAPCMU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.218"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..986)
                     /locus_tag="RD744_002690"
     CDS             complement(<1..986)
                     /locus_tag="RD744_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDU0558649.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="RD744_002691"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="RD744_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDU0558650.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..>2796
                     /locus_tag="RD744_002692"
     CDS             1811..>2796
                     /locus_tag="RD744_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDU0558651.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRL"
BASE COUNT          864 a          560 c          569 g          803 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctgga
//
LOCUS       DAPCMU010000211         2768 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPCMU010000211 DAPCMU010000000
VERSION     DAPCMU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.219"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            14..199
                     /locus_tag="RD744_002693"
     CDS             14..199
                     /locus_tag="RD744_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558652.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            211..459
                     /locus_tag="RD744_002694"
     CDS             211..459
                     /locus_tag="RD744_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558653.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            490..669
                     /locus_tag="RD744_002695"
     CDS             490..669
                     /locus_tag="RD744_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558654.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(712..1182)
                     /locus_tag="RD744_002696"
     CDS             complement(712..1182)
                     /locus_tag="RD744_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558655.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1269..1967
                     /locus_tag="RD744_002697"
     CDS             1269..1967
                     /locus_tag="RD744_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDU0558656.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2145..2486
                     /locus_tag="RD744_002698"
     CDS             2145..2486
                     /locus_tag="RD744_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558657.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2479..>2768
                     /locus_tag="RD744_002699"
     CDS             2479..>2768
                     /locus_tag="RD744_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDU0558658.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1019 a          401 c          561 g          787 t
ORIGIN      
        1 aggagctatt tttatgtctg agaatttaga cttaaaaatt cgagcggaga tgagaaaaag
       61 aagaatgact ttcaaagaac tagctgcgct tatcggtatt tcaggagctt atttatcaga
      121 tattctaaac ggcaatcgtg atggaaagaa agcacaacag catatcgaaa cagtgaaaaa
      181 aatattggac atccgatagg ggggtaggcg atgacaaaac taaaaaaaca agattttgta
      241 aaaaaataca attattctcc atctacttat caacgtcgaa tgtcggaact aaaaaataca
      301 gcaattttct cagcggcgta tgaacgggtc acaggacaag aagtttggat caatacagaa
      361 ttatacgata aatttttgtc tttcaaatcc tataacaggt tacgcacaag aaaggtaaca
      421 cctaaagaat ttatcgagaa gcatttagtt gatttataaa aaacagaaca ttttgagagg
      481 tgaaggttaa tgggtaaatt caacagagca ttggtattca gcgcaccgct aatcatctac
      541 gctttaggac tttggggaag caggcaagcg ttgataggaa cgattgttta catggtttgg
      601 atttttatgg ggcttgatga agctgaagct gagtacagag cgaaaaagcc aaccgagggg
      661 gctgactaaa aatgaataga aaagagaaac tagaatgtat attactatta ctcagtttaa
      721 ttctttcact aatttctcta ttgggaagtt tgtatttttg atatcaacaa ttttgtggat
      781 ttgggttcta tttagagtaa atatcatttt tgtttggtga ttaaggagtt tattaaaatt
      841 aattttccct acttcaaatg caaggtaaca agatatagca cttttaggag gaatatttag
      901 cggtacagct tcagagtata ttctattatc atcgactgat cctcctttag caattaattt
      961 cttaaatgga gtagctgacc atctgcggtt taagttagcg gatgaaagtt ctaaattaac
     1021 taacatagct ggttctgttg aatagttaga aatgattact tttgtataaa tcatatctgc
     1081 agcgaagtaa gaagcattaa gttcaacttc tagttgaggg cgcattcctt ttatttttat
     1141 ccctgttaaa aacgtactca atataaaact tattattgac atccattgaa aaattgttaa
     1201 gtgaaaaaaa ttcaaaataa acaccaccag tttttaacta aattatacca aaaaggagag
     1261 aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtgact acgagcttac
     1321 aagtggcaga aagcttcgag aaaaaacatc aacatgtttt aagagatatt gatgcactaa
     1381 aaaaagatgt gtccaatttt ggacagatgt ttgtagaagg taatgaacca gattcatatg
     1441 gcagaaatcg acgagttttc ttcattagta gagatggatt tttcttgctg gctatggggt
     1501 ttacaggaaa gaaagctatc tacttcaaac aaaaatacat tgaagcattc aacgaaatgg
     1561 aagatgttat tcgcaagaat actgttcctc aaacaattga agatatgatg atctatcaat
     1621 tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaagatact atgcgaatta
     1681 gcggacaaca agagtttgaa attaagcaaa aaggaaatat gaaagttatg ggagttctag
     1741 ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca aaattttggt
     1801 ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt aagagattcg
     1861 atgatgctgt ttcatttatt gaaatgtggt tgccagaaac tgcgatccgt atggaaattg
     1921 atcaactgaa cagacaacag agactttttg gtgatgacaa tgaatagagc tgaagcgcta
     1981 agaataggga cggcaattgc taattgctgg tggaaatact ataaaccaat catcctaagc
     2041 caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact ccgccggcaa
     2101 gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa aaagaacttt
     2161 ccactctaga tcaatatttg attgatcctg attggggcaa gccgaaaatt gaggaaacaa
     2221 gtggtcgaaa aatcagacga aatcttttga cgaatgaaga actagcttgg gatcaagatg
     2281 atttaggcaa ccatgtaact atttgggatc atgtttatct tatccatcta tcgaagcatt
     2341 cgaataaacc tgaatatatt tacgtcatcg aagatggctt gattgatgcg ctagaagagt
     2401 acgacagaga taacttgatt gatatctctt attacggacc aggtaagaaa tacattgctg
     2461 aaatggaggc agaatttgat gagtgaaatc aaagggacaa cgaactttga aaaacttttt
     2521 agtcgtaagt taaataaaat tctcaagaaa aaaggaaatt ttgattattt atcttgggct
     2581 cacgcgtggg agattatgaa aaagaatgat ccacaggcaa cggtaactat taatgagtac
     2641 aaacactaca gggttgtttc tggaactcat caagactttc ttgttgagga atataaacct
     2701 tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa aggacacacg
     2761 gaaaccga
//
LOCUS       DAPCMU010000212         2739 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPCMU010000212 DAPCMU010000000
VERSION     DAPCMU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.220"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="RD744_002700"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="RD744_002700"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDU0558659.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAPCMU010000213         2726 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPCMU010000213 DAPCMU010000000
VERSION     DAPCMU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.221"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(76..1170)
                     /locus_tag="RD744_002701"
     CDS             complement(76..1170)
                     /locus_tag="RD744_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDU0558660.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="RD744_002702"
     CDS             1359..2120
                     /locus_tag="RD744_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDU0558661.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="RD744_002703"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="RD744_002703"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDU0558662.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          861 a          547 c          464 g          854 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatca
//
LOCUS       DAPCMU010000214         2669 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPCMU010000214 DAPCMU010000000
VERSION     DAPCMU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.222"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            464..670
                     /locus_tag="RD744_002704"
     CDS             464..670
                     /locus_tag="RD744_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDU0558663.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            670..921
                     /locus_tag="RD744_002705"
     CDS             670..921
                     /locus_tag="RD744_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558664.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            936..1373
                     /locus_tag="RD744_002706"
     CDS             936..1373
                     /locus_tag="RD744_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558665.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1366..2073
                     /locus_tag="RD744_002707"
     CDS             1366..2073
                     /locus_tag="RD744_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558666.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2454..2633
                     /locus_tag="RD744_002708"
     CDS             2454..2633
                     /locus_tag="RD744_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558667.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          964 a          452 c          558 g          695 t
ORIGIN      
        1 cattttacgc gatcgatggg catataaaag aagagctgca gtcctaaatg aggattgcag
       61 ctctttttgt tttacataga aacaggaaaa gaccttttta ggaagacaca agtgccttct
      121 caattgaaaa acctttctct cactccaacc gccaccgccg ttcgagaaga aaggaagtga
      181 acgttgcacg ttcactaaga tatggtgtct tgcaaagcaa gacaaaatag aaaaaacttc
      241 ctctcaccaa aaggttcgtt cctgttcgct atctctttat tgatttgcca gaccggcaac
      301 aagttgcctt ttatcaccaa aaagcgacaa ggacaagggt aaatggcacg ctgcgcgccc
      361 ttgtccttgt cgcttttcgt tgattcattc tggccaacaa atcaataaag aaagaactaa
      421 cgaaaggaaa taaaaccttt cctaagagga ggcagaatca atcatgcgaa agaaatttga
      481 ttattggggt gttcccttca gcgaattatt cccaaactat catgcaccac atactgtcga
      541 atgcgattgt ggagaaagag cgaaatgtat caaaagctat cgtttgtatc aatgtccaac
      601 atgcgggaaa aaatatacct tgtcttatgg cgattatgtt ttgattgatg agaaagtaaa
      661 gaaacggtga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt tcaaattttg
      721 gtggacaaag aagtcatttc tgaaaaagta gcagaagctt tacttattcc gcggtactgg
      781 acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga cctagtgaag
      841 attgcaggat atgaactgca ttatcaagaa aacatggaaa cttatgaaat cgaatattgg
      901 ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac tatgacgaat
      961 ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg ttcctatacc
     1021 atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca gtggaagaaa
     1081 tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca ctggaagaac
     1141 tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa gtaggtgtca
     1201 aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat gcaaaattcg
     1261 cacagattaa gaaagctaaa gcaatggcta gcgactggct agatactttt gaaaatatcg
     1321 aaaaaaatgg tgcattttat acgattgaat cggaggagaa agataatgaa taaggtggtt
     1381 tatcagctaa caacagaaga tgtttcgcaa tttttaacaa acaaagaaca cttgaatgaa
     1441 gtaatcagac ggattcaaaa agactttagt tgtatggaag aaatgaagta ctttattcaa
     1501 agagtggaag aacagttgta tatggaaagt gaaacgagtc aaacgaaggt tacagtcagc
     1561 cataatcgtt tagacgtgta taaacgtcgt cctgacacat taaaaaaagg agcggagctt
     1621 ctacgactag aaacagaaaa atatatcatg aatgtttacc tagaaaggag tgagctggtc
     1681 gaaaactttg tcagctacac gatttgcagg aaacactatt actcagaatc agaatattat
     1741 tcaatttttg agagcatgga attaatggaa gaagaaatgg aagagctaaa cagcaaagat
     1801 gcgacggtag acgattatta ccttgtattt actagatttt tgacgtgtgg gacggaatct
     1861 atcgtgttaa tgggaatgga ttatgaactc actgtacccg aagcattttt gagggaatat
     1921 gtagaagaag tagataaccc aggcgacgac tggtacacga ctttaactga ttttttagac
     1981 acctacactt acgacgatac agaagaaatc caaaatgaac ttgaccgtaa acgaatgcca
     2041 tatcaacgat ttgaaaggaa aaaaggtcaa taaccattta taccgaaaag tgaggatgtg
     2101 tgaaataaga acaaaaaacc ttgaaattaa gggaatggtt cttatttcgc accaatcgat
     2161 ccaaccgccc ccgccgctta ggtggtgcga aataagcggg caaagcccga taacctaaga
     2221 gaaagaagga gatccatatg aataaaatca ttcctctatt gttcgtactg tttgttggcg
     2281 agatacttat gcgtcatggt gcagctctac atttaaactt cgtagtattc ttaggaatac
     2341 tggtattttg tttgatcttt tggcgctttg tgttaaccct tgtgagtcta atattcaagt
     2401 tgattgcagc catattttta atagggttat ttcgatgaaa ggagagagta ctaatgccaa
     2461 ttgcaatagg aaataaacgg ttacccgtga cgttggatga aaagagacaa aaagaattgc
     2521 agcaactaaa gcagaagtac ggcaaaagtg aatccaggat tatgtgtatt gcgttagatt
     2581 tattgattgc ccaagaaaaa gcaggatttg aggtaccagc actcaaaaag tgacgtcact
     2641 ttttatccta aaaactaaaa gtgatagca
//
LOCUS       DAPCMU010000215         2597 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPCMU010000215 DAPCMU010000000
VERSION     DAPCMU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.223"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            39..1451
                     /locus_tag="RD744_002709"
     CDS             39..1451
                     /locus_tag="RD744_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDU0558668.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="RD744_002710"
     CDS             1800..2432
                     /locus_tag="RD744_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDU0558669.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          967 a          376 c          511 g          743 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgat
//
LOCUS       DAPCMU010000216         2566 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPCMU010000216 DAPCMU010000000
VERSION     DAPCMU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.224"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            10..1017
                     /locus_tag="RD744_002711"
     CDS             10..1017
                     /locus_tag="RD744_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDU0558670.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1029..2033
                     /gene="neuB"
                     /locus_tag="RD744_002712"
     CDS             1029..2033
                     /gene="neuB"
                     /locus_tag="RD744_002712"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDU0558671.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2030..>2566
                     /gene="neuC"
                     /locus_tag="RD744_002713"
     CDS             2030..>2566
                     /gene="neuC"
                     /locus_tag="RD744_002713"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDU0558672.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTI"
BASE COUNT          937 a          332 c          514 g          783 t
ORIGIN      
        1 gagatataag tgtttaaagg aaaaacaata ttagtaactg gcggaactgg ttccattgga
       61 agtgaaattg ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat ttatagtaga
      121 ggtgaagaaa aacaatttta tatgcaacaa gagctctcaa atcacactaa tttgagattt
      181 ttaattggtg atatcagaga ctctcaaagg attgattatg catgtcgtga tgtggatatt
      241 atttttcatg ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc tatggaagct
      301 gtaaaaacaa atgtgatggg aactcaaaat gtaatagatg cagcgatcaa aaataatgta
      361 aaacattttg taggtatcag tacagataaa gtagtgagtc ctactaacac catgggtgcg
      421 acaaagttat tgagtgaaaa attaataata gctgccaatc agtataaagg aaaaacagaa
      481 acaatatttt cttgtgtaag gtttggaaat gtcatggggt caagtggatc tgttattcct
      541 ctttttagta aacaaataaa aaacaataaa gatattactt tgacagatgt tgatatgaca
      601 cgatttatga tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca
      661 aaatttggag aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa
      721 tcattaataa aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt
      781 gtaggtatca ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa
      841 cgctcatatg aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa
      901 ctgtacaaag attatgaaaa aattaaaatc actgcatact cttcagatgc aacagaatta
      961 atgagtggac aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga
     1021 gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa
     1081 gtctagattt agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta
     1141 aatttcaaac atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc
     1201 agaaggataa tcttaaagag gacagttctc aatttgaaat gctaaaaaaa ttagaattat
     1261 ctcaatctga ttttattaga ataaaagatt attgtataca aaataaaatt gattttttgt
     1321 ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta caaggattta
     1381 aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat atgtatgggt
     1441 tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag gctgtagatt
     1501 gtttcataga tagtccagtt actattttac attgtacttc aaattatccg gctagttctg
     1561 aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata cccgttgggt
     1621 actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg
     1681 taattgagaa acattttacc ttggataagg aactacctgg tccagatcat aaagcttctt
     1741 tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa atatttttag
     1801 gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta tcaagaaaaa
     1861 gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa tctcttacaa
     1921 taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt ggcaaaaaag
     1981 ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat
     2041 acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg ctgagaattt
     2101 actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat cccataaata
     2161 tggatttact aaagaattga tcttagctga cggatttaga attagtggag aagttcaaag
     2221 tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag agataaatgg
     2281 tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag gggatagagg
     2341 agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag cacatattca
     2401 cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa cgaaaatagc
     2461 atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga
     2521 ttcttggaga gtttttcccg tcggagctcc tagaatagat actatt
//
LOCUS       DAPCMU010000217         2516 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPCMU010000217 DAPCMU010000000
VERSION     DAPCMU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.225"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..180)
                     /locus_tag="RD744_002714"
     CDS             complement(<1..180)
                     /locus_tag="RD744_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDU0558673.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINN"
     gene            complement(193..1296)
                     /locus_tag="RD744_002715"
     CDS             complement(193..1296)
                     /locus_tag="RD744_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDU0558674.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1386..2342)
                     /locus_tag="RD744_002716"
     CDS             complement(1386..2342)
                     /locus_tag="RD744_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDU0558675.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT          633 a          520 c          449 g          914 t
ORIGIN      
        1 attattgata gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt
       61 gtttagccaa tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg
      121 aaatacacta gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat
      181 ggttcgtact ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga
      241 taatttgaag gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa
      301 gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc
      361 cattgaaaag actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt
      421 gacacttgcg atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta
      481 ttttttgcca tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt
      541 ccgttcttta atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac
      601 tgtccagtct tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa
      661 gtcaacgatg gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat
      721 gtaacctttg gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt
      781 tttttctttg tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat
      841 ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc
      901 accaatcgcc atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac
      961 taacgtgtag acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc
     1021 atcgatctca gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc
     1081 gaaggcaggt ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt
     1141 ccctaacaag aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact
     1201 ttcagtggtt gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc
     1261 cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa
     1321 ggtgagacga ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta
     1381 tgtgattatt tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca
     1441 gctgcattca ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct
     1501 gtaacaactt ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg
     1561 tcgttcagag tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca
     1621 ttatattctg tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat
     1681 tcagttactt tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag
     1741 ttcttttctt caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt
     1801 gtatctgtgt taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt
     1861 tcagatgagt gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag
     1921 aattggtcat tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg
     1981 tttttgttgt attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat
     2041 tttgattcag tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt
     2101 gaataatttt tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact
     2161 tttgcagatg attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa
     2221 gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat
     2281 gctaatacgg caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc
     2341 atttctagtc cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata
     2401 agtcaactat ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt
     2461 tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaa
//
LOCUS       DAPCMU010000218         2499 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPCMU010000218 DAPCMU010000000
VERSION     DAPCMU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.226"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(883..1401)
                     /locus_tag="RD744_002717"
     CDS             complement(883..1401)
                     /locus_tag="RD744_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDU0558676.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            1768..2016
                     /locus_tag="RD744_002718"
     CDS             1768..2016
                     /locus_tag="RD744_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558677.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT          817 a          453 c          367 g          862 t
ORIGIN      
        1 tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt agataacacg
       61 aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt
      121 gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt
      181 gcagaaatcg gtactgggat taaactaagc aataaaaact aatgttcccc tcattctttg
      241 aacttctact atttgattta tttattctga taaatgacga tttcaccttc aggatcttca
      301 aatcccgtat agaatttatt taagatctct tcagtatcag taataggata ggcgataggc
      361 cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag
      421 catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat
      481 ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat
      541 gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa
      601 catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata
      661 tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat
      721 tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc
      781 aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac
      841 aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt
      901 ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa
      961 tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc
     1021 agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg
     1081 attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt
     1141 atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc
     1201 atttctttca tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa
     1261 attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat
     1321 agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc
     1381 tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt
     1441 catctataat ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt
     1501 gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat
     1561 gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac
     1621 tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta
     1681 ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca
     1741 caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta
     1801 cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac
     1861 gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc
     1921 gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt
     1981 aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca
     2041 caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta
     2101 atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat
     2161 tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg
     2221 tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt
     2281 aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga
     2341 cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc
     2401 tcatttcaac ttatatgttt tagtgaacta cccccactta gcttggatag gctacaatta
     2461 actagacaga aaaattaagg tgtgtagact agaagaaaa
//
LOCUS       DAPCMU010000219         2492 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPCMU010000219 DAPCMU010000000
VERSION     DAPCMU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.227"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            189..2342
                     /locus_tag="RD744_002719"
     CDS             189..2342
                     /locus_tag="RD744_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDU0558678.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          857 a          428 c          547 g          660 t
ORIGIN      
        1 aaaaataagt ttatttttta atgaaaaaaa tagttggata tgtaacttta agtgattgct
       61 gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt
      121 ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag
      181 gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa
      241 taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc
      301 gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg
      361 gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga
      421 ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag
      481 ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag
      541 atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc
      601 tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca
      661 caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg
      721 tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca
      781 tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg
      841 ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga
      901 ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca
      961 gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag
     1021 gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc
     1081 tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc
     1141 aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat
     1201 tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca
     1261 aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc
     1321 tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag
     1381 gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact ttgacagcag
     1441 gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctgc
     1501 aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag
     1561 tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag
     1621 gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc
     1681 agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc
     1741 cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata
     1801 atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga cagtttgaca
     1861 catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc gggaaacgag
     1921 tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag
     1981 tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg
     2041 cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca
     2101 aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg
     2161 cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac ggaccaacag
     2221 acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca
     2281 taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat
     2341 aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat
     2401 agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg
     2461 tttactcggc aaaaaaggtt gtgacaaaaa tt
//
LOCUS       DAPCMU010000220         2484 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPCMU010000220 DAPCMU010000000
VERSION     DAPCMU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.228"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            228..602
                     /locus_tag="RD744_002720"
     CDS             228..602
                     /locus_tag="RD744_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558679.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            607..1035
                     /locus_tag="RD744_002721"
     CDS             607..1035
                     /locus_tag="RD744_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDU0558680.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1126..2040
                     /locus_tag="RD744_002722"
     CDS             1126..2040
                     /locus_tag="RD744_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDU0558681.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            2156..>2484
                     /locus_tag="RD744_002723"
     CDS             2156..>2484
                     /locus_tag="RD744_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0558682.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETY"
BASE COUNT          948 a          389 c          422 g          725 t
ORIGIN      
        1 tgacaataac taatattttt attagtatat agacatagct aaataagaca cagaaatgcc
       61 tagtaattta catttcagag tttgccgacc gtgaagtgtt tattagttat caagtctttt
      121 tagctaatat tttagcttac gaaaacagta tactaacact tttattagtt gtcaacacct
      181 ttaactaatt tttttattag tgttttcgta gcttgtgagg tgcgaaaatg aatttactag
      241 atagaataaa ggaattagct cataaaagag gcataagtat aactcaattg gaagaagaat
      301 taaatatacc taaaaacacg atttatcaat ggaaaaatag aactcctagt actaaaagac
      361 tacaacttgt agcagattat tttaatgtaa ctactgatta tttactcggt agaaaccaag
      421 ttcctgactg ggcaacaaaa gatgaagtag ttgaacttga taaattacta gattcaaatg
      481 tcaatatgtc ttatggcgga gaaacactga cgcctgaaca ggtccaacgc gtaaaagata
      541 tccttattgg aaccttctgg gatattgtga aagaagacaa agaaaaaggc aaaaagatgt
      601 gagcttatgg agatggatac gattaattta gtcgaggagt tgaagcggaa ataccagtcc
      661 gctaatcctt tttatatttg tgaaaagatg ggcattaaaa ttcaatacgt tccttttatc
      721 gagaatccca aaggacagtt tcaagaaatt agaggtcgtg caataatctt cttaaatgat
      781 gaactgagag actctgagga aagattctac atttgcgctc acgaattagg tcacgctatt
      841 tttcatcgtg gcttatccag ttactatgta tcaacaagaa catctagaag caaatctgaa
      901 agcgaagcta attgctttgc tgctaatctc attgtttctc tatacaaaga agacaatgat
      961 caatatccta aacgaattga ggaattaaag aatctttacg gacttccaga aagcgcttat
     1021 cgctttctta tataatgtaa aaaaagtccg tgctgcaaca cggactaaaa accttatttc
     1081 taagatccta caaaaaaatc atatcataga agtgaggaat taaaaatgaa aaaaattgtt
     1141 tttgggttgc tttttttagg cttggtatta agcggatgct ccactagtcc taattctgct
     1201 agcactaaag aaaatactgt ttcatcaaca tctgaatatg aaacaaaaaa actaacttca
     1261 tcagacgttc ataaaataaa agttggtgat gatttaactc aagtttatca gacacttggt
     1321 tcaccgttac gtaaatggac aaatgatttt gtttacaaag aattagacaa tgctatcaca
     1381 cgggatgaat taattgtatc tatttcaaag gatgcacaag gagattatta taaagagcac
     1441 tctgaattaa ttgaacatgg aaatagtgca aaagatataa aaaaattaga gatgctccaa
     1501 tatacttatt caactgattc tgatgatgaa acaatgttaa tttgggttag tccaaaaact
     1561 gaacaggttg tatattataa tcaacgaact ttcttagata aagatggaaa atccaaagat
     1621 tcaagctcta ctacaactac aactacaact tcgtcttcag aaccccagac ggcatcagtt
     1681 gcacatagta taggagaagc agttcctttt acttctgaaa atggtaactc tattaacgta
     1741 actgtccagt ctgtatcaaa agattacggt gatgatttct ataaaccaga aggcctattt
     1801 tacgcaaagg tagattttct tgtagaaaac acagggacta caccttttga tgcaagttca
     1861 agttatttag aattttatga ttcatcagat attaaatctg atttagctag tagagattac
     1921 ttttcggaaa ccatccaacc gggtaaatcc gcacaaggaa cagcatattt tgaagtgaaa
     1981 aatgatggaa caacatttga agtgttcttc gcagatactt catggaaagg tagctactaa
     2041 acaaaaaaac acgccccctc cctcgccaaa gtttgtggac gtgatgaaaa ataaacctgc
     2101 tataattggc ttacttatcc attcctatta tagcaacaaa taggagatga aaacaatgtg
     2161 gattgaaacc aaaactgata aaaacggaaa aaaagtatat aaatataatg agcgatatat
     2221 tgatccaaaa actagaaaaa gaaaaaaagt gagcattact tataaaaata aatctcgaga
     2281 aactcagaaa gtggcattgc tagagttaaa taaaaaaatt gatataaaat taaacgaaaa
     2341 aacacttcag aagcctgatc taacatttca tgagcttgtc gaagaatggt tagttattta
     2401 caaaagacaa gttaaggagt ctacatatta tcctacgaat aatatcttaa acactataaa
     2461 gaaaaagata cctgagactt acat
//
LOCUS       DAPCMU010000221         2471 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPCMU010000221 DAPCMU010000000
VERSION     DAPCMU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.229"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(13..1146)
                     /locus_tag="RD744_002724"
     CDS             complement(13..1146)
                     /locus_tag="RD744_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDU0558683.1"
                     /translation="MAKFIIKSAEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIVSY
                     RDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGATD
                     MKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDALII
                     GEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHLAE
                     TIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVNEL
                     NQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKANH
                     SFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            1226..2305
                     /locus_tag="RD744_002725"
     CDS             1226..2305
                     /locus_tag="RD744_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558684.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          699 a          565 c          539 g          668 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgtccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctcg gcggatttta taatgaactt
     1141 agccatttaa aataaaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
     1201 tctcaataga caaggatgaa ttcacatgac gcaaacccat gataacacag tttcacgcaa
     1261 gggaaaacat ctttcctact cagaacgttg ccaaatcgct atcctgaaaa aggaaggcta
     1321 ttccaaccgt caggttgcca aagtgctggg acgtgttccg caaaccataa acaatgagat
     1381 tgatcgcgga accatcaccc agttaaaacg ccaaacgcaa aatggaaaag tgtatgacac
     1441 ctactattct gtttatgacg ctgatgcagg acaggccttc tatgaaaagc aacgactgaa
     1501 ctgtggcaga cgccccaaat gggcgaacac aaatgccttc attgattggg cagatgacaa
     1561 aatgttaaat gaaaaatggt cgccggacgt cgtggtcgga tttgccagaa aacacgagct
     1621 gttcgattgt gccatcattc ctggcacaac cgctctttat caatggattg accgcggaat
     1681 catgcgaaca aagaacatgg acttgcttga aaaactgtcc cgtaaaatca aagcggtccg
     1741 cccaggacac cgtcccaaca agcgtgttct cgggacgtca attgaagaac gccctaaaga
     1801 agttgaaaca cgggaaacgt tcggtcattg ggaaatcgac acggtagtgg gaaacaagac
     1861 caagtcggat gccgtgctgc tgacgctggc tgaacggcag acacgctttg aagtcatcct
     1921 aaagttgaac gggaaggaca ccgaatcggt ggaccaagcg gttcaacagc taagagaacg
     1981 ggctggcgct aacttcgatc aattatttaa gaccgttaca tccgacaatg gctcggagtt
     2041 ttctgggcta cacgatgctc tgcaagatgt agctgacgtc tatttcagcc acccctatgc
     2101 ttcttgggag cgtgggacga gcgaaaacca gcacaaatta attcgccgtt tcgtccccaa
     2161 gggaagcccg attagtgagg tgagcgagaa ccagattttg cggatccagc gttggatgaa
     2221 cgattacccg cggaaaattt tgggttatgc gacaccacat gatacattcg tccaggcatt
     2281 caagcaagaa cggttggtgg cttgacccat tattccttga taacgatcct cactcgggac
     2341 atggcttcat gtctggaaag ctggcctggg gccagaatcg tctgaagact atcatacctc
     2401 agttcattat caaggcctca ctgatttaga ataacgaggc agttacggta tcaaacaaat
     2461 gagttacctg t
//
LOCUS       DAPCMU010000222         2436 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPCMU010000222 DAPCMU010000000
VERSION     DAPCMU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.230"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..272)
                     /locus_tag="RD744_002726"
     CDS             complement(<1..272)
                     /locus_tag="RD744_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558685.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="RD744_002727"
     CDS             complement(309..446)
                     /locus_tag="RD744_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDU0558686.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="RD744_002728"
     CDS             complement(448..894)
                     /locus_tag="RD744_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558687.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="RD744_002729"
     CDS             complement(891..1040)
                     /locus_tag="RD744_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558688.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="RD744_002730"
     CDS             complement(1054..>2436)
                     /locus_tag="RD744_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558689.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DAPCMU010000223         2436 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPCMU010000223 DAPCMU010000000
VERSION     DAPCMU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.231"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(966..1256)
                     /locus_tag="RD744_002731"
     CDS             complement(966..1256)
                     /locus_tag="RD744_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558690.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1464..2156)
                     /locus_tag="RD744_002732"
     CDS             complement(1464..2156)
                     /locus_tag="RD744_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDU0558691.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          712 a          450 c          347 g          927 t
ORIGIN      
        1 taatttccat agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct
       61 tgaactccat gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct
      121 acttgcataa actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa
      181 gataccccaa atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt
      241 atgtttgttc atttttatac tccttcttat atttcctaat agagtataac atagctttaa
      301 gcattactta aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat
      361 ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt
      421 tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa
      481 gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa
      541 aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc
      601 ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca
      661 ggaacgttta aaatcctaca attccactac ctgagactag taatgactag tctcttttta
      721 tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc
      781 tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa
      841 tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc
      901 ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt
      961 gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca
     1021 tattctacca caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt
     1081 ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag
     1141 ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt
     1201 ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc
     1261 ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta
     1321 tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat
     1381 gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat
     1441 ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat
     1501 ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc
     1561 aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt
     1621 tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta
     1681 ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc
     1741 agatgattga tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt
     1801 tgtttgcttt gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct
     1861 gctgccgatt gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca
     1921 tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta
     1981 agcgcttgct ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta
     2041 tcaatatctg tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag
     2101 ccaattagac atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc
     2161 atagctcctt aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca
     2221 agcgttttta aattacatgt gtaattttaa tataaatact atcaaatact catttaatcc
     2281 tcctttttta tctatttaaa aaatcttttt tatagagaaa aatgcaggtt aatactttct
     2341 taaataaata tttatggtga acaaaaacaa aaaagcaagt tttattaaac gtaacccttt
     2401 tcatttcata gattactgta tttattacaa aaaacg
//
LOCUS       DAPCMU010000224         2407 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPCMU010000224 DAPCMU010000000
VERSION     DAPCMU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.232"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            5..1150
                     /locus_tag="RD744_002733"
     CDS             5..1150
                     /locus_tag="RD744_002733"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDU0558692.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="RD744_002734"
     CDS             1321..1779
                     /locus_tag="RD744_002734"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDU0558693.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="RD744_002735"
     CDS             1938..>2407
                     /locus_tag="RD744_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDU0558694.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAPCMU010000225         2400 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPCMU010000225 DAPCMU010000000
VERSION     DAPCMU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.233"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            207..2069
                     /locus_tag="RD744_002736"
     CDS             207..2069
                     /locus_tag="RD744_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0558695.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="RD744_002737"
     CDS             2069..>2400
                     /locus_tag="RD744_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0558696.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAPCMU010000226         2385 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPCMU010000226 DAPCMU010000000
VERSION     DAPCMU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.234"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(4..249)
                     /locus_tag="RD744_002738"
     CDS             complement(4..249)
                     /locus_tag="RD744_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558697.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="RD744_002739"
     CDS             complement(266..1117)
                     /locus_tag="RD744_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDU0558698.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="RD744_002740"
     CDS             1283..2287
                     /locus_tag="RD744_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDU0558699.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          762 a          403 c          481 g          739 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa gagcg
//
LOCUS       DAPCMU010000227         2378 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPCMU010000227 DAPCMU010000000
VERSION     DAPCMU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.235"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            259..615
                     /locus_tag="RD744_002741"
     CDS             259..615
                     /locus_tag="RD744_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0558700.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            625..1524
                     /locus_tag="RD744_002742"
     CDS             625..1524
                     /locus_tag="RD744_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDU0558701.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1921..>2378)
                     /locus_tag="RD744_002743"
     CDS             complement(1921..>2378)
                     /locus_tag="RD744_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558702.1"
                     /translation="GNKTKSDAVLLTLVERQTRFEVILKLAGKDAESVNQAVRQLRER
                     AGAYFGQLFKTVTSDNGSEFAGLHDALQDVANIYFSHPYASWERGTSENQHKLIRRFI
                     PKGSPIGNVSENQILRIQRWMNDYPRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          707 a          485 c          525 g          661 t
ORIGIN      
        1 tgttaaaggt tttttatttt ttatagtatt ttttgttatt taagaaagga ataaggttaa
       61 acaaataaca ggttgctttt tgggtctttt tccgttcaag aaactgaacg aagaaaaggg
      121 aaagttgtct cttttgtgat aagagaaatc ataataaacg aatgacttac tttccaataa
      181 attgagaaca gtcagaacca gacaaattag ttgcatctga tgaataaagt gattattcta
      241 tgacatgagg tgatacagat gaaattagaa gtgacaaaag aagcacaaga agtattgaag
      301 aataaattag aagcagacga tcaactttta ttagatatcg aaaacggtga cggtccattt
      361 gcagaaagcc aagtcagttg ccaattggat acggcatttc gattgatcat tgtcaaaaaa
      421 gatacacaaa cagatctaag tctttattct gtagtggctg atacaccagt tggaccaatc
      481 aaaatcaaag acagtgcaat actttacatg gatgatccat ccacacttgc tttagaacca
      541 acttataata gtttacaatt aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt
      601 gttcgaaaag actagaggga taaaatggga aaattggcga tcatcagcga cctacatgtg
      661 gacatcaata aattcggcga atcagagctg atgcagcttt ggcaagtttt gcaaaagcac
      721 aagatcactc ggcttcatct agccggagat acggctaata aagtagaccg ttgcctagct
      781 gttgtcgaat ttttcgtcaa taaaggatta tctacgacat ttcatttcgg caatcacgag
      841 ttggcagatg tgcccaatga acagatgatg gaccattttc cagacccaca ttttttaaat
      901 gaacggtata ttgctttaaa tgagcagacc gttcttttag ggatgaatgg ctggtacgac
      961 tatcagtttt ccgaattgaa agattacgag gcaatcgtac gaatgaagcg tttgtattgg
     1021 tatgatcggt tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca
     1081 gctgcaaaaa atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat tttatccact
     1141 catttcgttc ctaaacgggc atttatcatg tatcaatctg ctccttatga gcgttggaac
     1201 aaattaaatg catttttagg atcagaatca ttcggagaat tacttgaccg ttattcaaat
     1261 atcaaacaag tagtttttgg ccatacgcat cgtcgctttg aagataaatc catcggtcac
     1321 acgatttaca gctgcagacc ttttggttat tattatgaat ggcagctgac ccgagatttt
     1381 gtcttgaaag aacagttgct agacagctat gatcctatga aattacgtgt cctcttgcgt
     1441 gcccatcacc cagcttttca ggcctatcaa acacaacatt tgcaagagga atttgaacaa
     1501 gcgatgacaa tcgttccgta ttgagacagc attagtaatg tgtaaaagaa aataccttgt
     1561 aaaacataag gataagtaag agcagtagat ccatttatcg tggatatgct gctttttttg
     1621 cggaaaataa tcagcgaatg tttatttgat cggatgattt gtaactcgca gagtactaga
     1681 tgcaagcgtg tattcttttt ttgaacgata atcacaaaaa aaaatataat cgtaaatttc
     1741 aagtttaagt tagccaccca tggtgacccg gttgctcgtt accaccaacc gtctaatttt
     1801 tctaatcgag tgggcccttg acaatgagct gaggcatggt agtctcccga ctggctggcc
     1861 ccggccagct ttccagacac aagccatgcc gagggaggct cgttgtcaag ggcccacgtg
     1921 tcaagccgtc aaccgttcct gcctaaatgc ctggacgaac gtgtcatgcg gcgttgcata
     1981 gcccaaaatc tttcgcgggt aatcgttcat ccaacgctga atccgcaaga tttggttttc
     2041 gctcacgttg ccgattgggc tgcccttggg gatgaaacgg cgaatcagct tgtgttggtt
     2101 ttcgctcgtc ccgcgctccc aggaagcata ggggtggctg aagtagatgt ttgccacatc
     2161 ctgcaaggca tcgtgcagtc ccgcgaactc cgagccgttg tccgaggtaa ccgtcttgaa
     2221 cagctggccg aagtacgcac cagcccgttc tctcagctgt cggaccgctt ggttgaccga
     2281 ttcggcatcc ttcccggcca gcttcagaat gacctcgaag cgtgtctgcc gttccaccaa
     2341 cgtcagcagc acggcatccg acttggtctt gtttccta
//
LOCUS       DAPCMU010000228         2340 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPCMU010000228 DAPCMU010000000
VERSION     DAPCMU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.236"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            328..945
                     /locus_tag="RD744_002744"
     CDS             328..945
                     /locus_tag="RD744_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558703.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            986..1285
                     /locus_tag="RD744_002745"
     CDS             986..1285
                     /locus_tag="RD744_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDU0558704.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1319..1486
                     /locus_tag="RD744_002746"
     CDS             1319..1486
                     /locus_tag="RD744_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDU0558705.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1531..2136)
                     /locus_tag="RD744_002747"
     CDS             complement(1531..2136)
                     /locus_tag="RD744_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558706.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          792 a          369 c          434 g          745 t
ORIGIN      
        1 taatagtgtt aaaaaaattg taaaagttaa agaagtcaaa ttctgatgtg aaaaataaaa
       61 tataccatgg aagacataaa agaaaaactg gataactagt ttatttaatg gcagaccgtt
      121 aaggcctgcc atttttatgt attttagcgg aataaatata atcatgcggg agtgtaaact
      181 tactgtttca aaaagtatac tttttgacat gccttgattt tctttgttta tgtatggcat
      241 aataaccaca taatacaatt tgaaacgttt ctgatttaat ctgattgttt tgaaacaatt
      301 ttagtgaaaa aagggtagat aaaaacgatg agaaaatttt tttatatccg ggtcagtagt
      361 tatgatcaat cgatcgaacg acaactgatt tccgcgaagg aattaggaat ccctgaagaa
      421 tatatatttg tagaaaaagc cagcggcaaa gattttaaac gccctgaata tcaattaatg
      481 aaacggatgt ttcgaaaagg agatttactc tatctacaat cattagatag actaggcaga
      541 aataaacaaa tgattttaga agaatggcga gaattaacta aggaaaagca gattgatatt
      601 tctgtgttag acatgcctct gttagatact gctagttaca agcaactaga tgggttagag
      661 acgcttatct ctgacttaat tctgcaatta ctcagctata tggccgaaga cgagcggaaa
      721 cgaattaata gccggcaaag agaaggcgta gaagctgcga aacaaaaggg tgtaaaattc
      781 ggcagaaaga aaattgcttt acctcctgaa tttcctgcga tctattctga ctggaaagca
      841 ggaaagataa ctgcagtcta tgctatggat caattgggca tgaaacgaaa taccttttat
      901 cgacgggtga aagaatatga acataccttg caccactttt cttgatagat ataacagcta
      961 ttagacggga agggagagtt tgaaaatgga cttaacgaat caaagggtcg tttttactgg
     1021 aacgctgcag caaatgacac gaacccaagc catcggtttg gttcactctt taaacggcca
     1081 ttgtcaatca agtgttactt caaaaacaaa tttcctggtt tgtggtcagt attctaagtc
     1141 gttgtttgat gattctctct atacaaaaaa ggaacaaacc gcgcgggact tgattgcgtt
     1201 aggtcatgag attaccattt tatcagaagt cgaattttat gcgttaatta gtcaacagtt
     1261 gaaagaatgg caacggtatt tataatcgtt agtaaaattt acaaggagga aaatttttat
     1321 gaagcgaaaa tcttatgatc gttggggcat tgaaataact agttttaatc gaaatattgc
     1381 ttttcacgtg gagtgtgact gtggggaatt agcaaaaagt gtttatcaaa aaaattactt
     1441 ttactgtcct aaatgccatc gccgatacgg ggtacatgga acatagaaac gaaatagtgc
     1501 ggtagaaaag cgaatgaatg tagtatattt ctacaaatca ttcgctttct ttattttata
     1561 aatagttggt cgagagatcc cgttttcttt tgcaatctct gcaactggaa tgccttgctt
     1621 taacatgtcc accacggttt taaaaactaa gcgtttttgc ggatctgttg agttaataga
     1681 atactcgatt ggtcgtccct tatatcggcc ggctttttga gccaataata ctccttgccg
     1741 ctgacgttca cgaattttct cacgctcgtt ttgtgcaata taacttagca gtgataaaaa
     1801 catatcgaac atagcggtat ccaggagttc atttccagta ttaaaatcta aaaactgtgc
     1861 atctaatatt tttaacgcta cttttttctg tttcataaaa gcaactgtat tttttatatc
     1921 ttcataatct cttcctaaac gatctagtga tgtaacgatg acgcaatcac cttcacggat
     1981 ataatttagt aatttttgaa actcaggacg ctcagtatct ttaccactgg ctttttcttg
     2041 gaataattta tcaatgttaa gtccttccat cattgccaat tgtcgatcca aattttgatc
     2101 ttgtgaagat actcgaatat agccaatttt tcccataaaa aactcctttt taaaagtgat
     2161 ataatttaca tgtaaaattt acttgtagtt ttactgctat catagcattt ttaaaaataa
     2221 attttatgta gacttaaaat ttacttcatg gagggtttaa atggatgaaa tcaaaagtaa
     2281 caaaaaagca tttggcattt atttaggaga acctagtaca gaacaattaa accaattttt
//
LOCUS       DAPCMU010000229         2336 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPCMU010000229 DAPCMU010000000
VERSION     DAPCMU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.237"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            10..>2336
                     /gene="esp"
                     /locus_tag="RD744_002748"
     CDS             10..>2336
                     /gene="esp"
                     /locus_tag="RD744_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDU0558707.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          849 a          370 c          476 g          641 t
ORIGIN      
        1 atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac aaagaaacga
       61 gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt gattggtgtt
      121 ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc tccaagcagc
      181 ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc ggggaatact
      241 tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca atttagtcct
      301 caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt ttacggaaaa
      361 cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga tgaacgatta
      421 gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc ttctagacgg
      481 acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa ggtcaattat
      541 attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca aaccgctcct
      601 aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat aggcatagac
      661 aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca agacgatgat
      721 aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga tgatttctat
      781 aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc agtaagtgca
      841 aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg tgctaatgga
      901 gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc atcttccgca
      961 aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc atatgtagcg
     1021 ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc gtttgaatcc
     1081 ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa ttatggcata
     1141 ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc aagtccacgt
     1201 ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat cttagaagat
     1261 atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt catctttgat
     1321 tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac aggttattat
     1381 tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag cggagacacg
     1441 aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac aacagttaag
     1501 gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact tgttgataaa
     1561 gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg ggctccaatt
     1621 tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat cgcgaaagat
     1681 gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg ttcaaccgtt
     1741 attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt taaaccagat
     1801 gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta ttcatggaaa
     1861 acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac tgttaagatc
     1921 ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca agctatggaa
     1981 aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca aagtggaaaa
     2041 cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat ttcagatgtc
     2101 tataataagt acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt tacattcact
     2161 ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat taaaattgtt
     2221 taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa agatatttat
     2281 gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac cgcagg
//
LOCUS       DAPCMU010000230         2261 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPCMU010000230 DAPCMU010000000
VERSION     DAPCMU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.238"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            278..820
                     /locus_tag="RD744_002749"
     CDS             278..820
                     /locus_tag="RD744_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558708.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="RD744_002750"
     CDS             976..1425
                     /locus_tag="RD744_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDU0558709.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2261)
                     /locus_tag="RD744_002751"
     CDS             complement(1455..>2261)
                     /locus_tag="RD744_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDU0558710.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          784 a          372 c          429 g          676 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgccg actgagttga agcccacgct ttgctaaaca cttcttcaat
     2161 tttcgggtac cgtaagcctt tcggtttcga ataaactctt cctgaacgat ctcttctaag
     2221 tcggactcat tttcgatcgg tttcgcttga taatagtagg t
//
LOCUS       DAPCMU010000231         2250 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPCMU010000231 DAPCMU010000000
VERSION     DAPCMU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.239"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            89..607
                     /locus_tag="RD744_002752"
     CDS             89..607
                     /locus_tag="RD744_002752"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDU0558711.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            735..1418
                     /locus_tag="RD744_002753"
     CDS             735..1418
                     /locus_tag="RD744_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDU0558712.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1727..1800
                     /locus_tag="RD744_002754"
     tRNA            1727..1800
                     /locus_tag="RD744_002754"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1761..1763,aa:Arg,seq:tct)
     gene            1959..>2250
                     /locus_tag="RD744_002755"
     CDS             1959..>2250
                     /locus_tag="RD744_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDU0558713.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLT"
BASE COUNT          764 a          342 c          451 g          693 t
ORIGIN      
        1 gggaaacatt tttacagcta taatttgaaa aaaatttatc gttattatat gattaagaag
       61 gattgaataa agaaagaagt tgaaaacgat acctgattac aaaaaaataa cttttataag
      121 ttctataacg ttgattatat ttttaattct tattcttctc gtcgtcttgg ttccccgaat
      181 ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg
      241 aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac
      301 atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa
      361 ccacgaagat ttttatacaa aagctgatac aaagacttat attgagcctg gagtagtaaa
      421 aaagagaaat attatcggta gtcccatctt tcagattcca tatattggac tagtcctttc
      481 aaaaacggca tttgcatgga ttcaggggtt attctttacg attgcttttt gcctaacagg
      541 aatcacagca tggaatacct ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt
      601 tttgtaagaa tgttaaaaac ataaatgtat tccagagatc caacgactct tctgaacatg
      661 aaacacgaga aaacactcat gcagcttgta gatttagtca gttgttcgta taatgtgtga
      721 agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat
      781 tggattttat gcgtacccag caaaaaaaat taccgggtga gctaggcaaa ctggaagaag
      841 aagctcatgt agcagaagta ccagttattc cccatgaaac agttgtattt ttgaaatttt
      901 tacttggaca gcttcagcct gaacgaatct tggagatcgg agcagctatc ggtttttcat
      961 caagtgtcat ggccacgaca atcggagaaa acgctcatgt tacgacaatt gatcgtttcg
     1021 atgtcatgat cgagaaagct aaaaaaaact atgaacgtct aggactgaca gataaagtga
     1081 ctttgcttga aggtcaagca gcagatatct tgccggagtt gtctggtccg tatgacttta
     1141 tttttatgga tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc ttacgtttgt
     1201 taagaaaagg tggcgttttg atggtagatg acatcttcca aggaggaacg atcttgctcc
     1261 cagatgaaga gattccccgt ggcaaacgag ctatccatcg taaattaaat gaatttcttc
     1321 gtgttgtgat ggatcatccc gatttgacat cgacaatctt gccattaggt gatggtgtga
     1381 tcttaatgac aaaggaatca gaaacaatcg agttgtgata catgattatg gcagcctgta
     1441 gataatcgat tgaatcgagg gctcaattcg aacttgatgt aaaagtttct atatttgtaa
     1501 gtcaattaaa ggatacagga aatctaaata aacaggagta gcatgaagtc aatgtagctc
     1561 ctgttttcaa ttgataacct tagaacagct gaaggagaat gtctcgtttt aaaagattga
     1621 tttagtagtt gggccaaaat aattcattat ttgctaatta aataactatt tcgtattgac
     1681 agaagcaatt taattatcta taattattga tgttccgctg attcttgtca tagtagctca
     1741 gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg
     1801 ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt gtgccaatat
     1861 tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca attataagga
     1921 gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta tctagaattt
     1981 tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg gtgagaactg
     2041 attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc agaaaatctg
     2101 ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg ggactacttt
     2161 atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta atatctattt
     2221 tttttgttgc attatatggc agtttgacta
//
LOCUS       DAPCMU010000232         2248 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPCMU010000232 DAPCMU010000000
VERSION     DAPCMU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.240"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(12..83)
                     /locus_tag="RD744_002756"
     tRNA            complement(12..83)
                     /locus_tag="RD744_002756"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Glu,seq:ttc)
     gene            complement(95..168)
                     /locus_tag="RD744_002757"
     tRNA            complement(95..168)
                     /locus_tag="RD744_002757"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Ile,seq:gat)
     gene            complement(181..251)
                     /locus_tag="RD744_002758"
     tRNA            complement(181..251)
                     /locus_tag="RD744_002758"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Gly,seq:tcc)
     gene            complement(256..328)
                     /locus_tag="RD744_002759"
     tRNA            complement(256..328)
                     /locus_tag="RD744_002759"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Phe,seq:gaa)
     gene            complement(332..404)
                     /locus_tag="RD744_002760"
     tRNA            complement(332..404)
                     /locus_tag="RD744_002760"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Asp,seq:gtc)
     gene            complement(423..496)
                     /locus_tag="RD744_002761"
     tRNA            complement(423..496)
                     /locus_tag="RD744_002761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Met,seq:cat)
     gene            complement(509..598)
                     /locus_tag="RD744_002762"
     tRNA            complement(509..598)
                     /locus_tag="RD744_002762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(560..562),aa:Ser,seq:tga)
     gene            complement(621..692)
                     /locus_tag="RD744_002763"
     tRNA            complement(621..692)
                     /locus_tag="RD744_002763"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Glu,seq:ttc)
     gene            complement(714..801)
                     /locus_tag="RD744_002764"
     tRNA            complement(714..801)
                     /locus_tag="RD744_002764"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Ser,seq:gct)
     gene            complement(826..899)
                     /locus_tag="RD744_002765"
     tRNA            complement(826..899)
                     /locus_tag="RD744_002765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(863..865),aa:Ile,seq:gat)
     gene            complement(913..983)
                     /locus_tag="RD744_002766"
     tRNA            complement(913..983)
                     /locus_tag="RD744_002766"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Gly,seq:tcc)
     gene            complement(985..1060)
                     /locus_tag="RD744_002767"
     tRNA            complement(985..1060)
                     /locus_tag="RD744_002767"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1025..1027),aa:Asp,seq:gtc)
     gene            complement(1079..1152)
                     /locus_tag="RD744_002768"
     tRNA            complement(1079..1152)
                     /locus_tag="RD744_002768"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1116..1118),aa:Met,seq:cat)
     gene            complement(1165..1254)
                     /locus_tag="RD744_002769"
     tRNA            complement(1165..1254)
                     /locus_tag="RD744_002769"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1216..1218),aa:Ser,seq:tga)
     gene            complement(1278..1351)
                     /locus_tag="RD744_002770"
     tRNA            complement(1278..1351)
                     /locus_tag="RD744_002770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1315..1317),aa:Ile,seq:cat)
     gene            complement(1365..1438)
                     /locus_tag="RD744_002771"
     tRNA            complement(1365..1438)
                     /locus_tag="RD744_002771"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1402..1404),aa:Met,seq:cat)
     gene            complement(1466..1538)
                     /locus_tag="RD744_002772"
     tRNA            complement(1466..1538)
                     /locus_tag="RD744_002772"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1503..1505),aa:Ala,seq:tgc)
     gene            complement(1558..1631)
                     /locus_tag="RD744_002773"
     tRNA            complement(1558..1631)
                     /locus_tag="RD744_002773"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1595..1597),aa:Pro,seq:tgg)
     gene            complement(1641..1714)
                     /locus_tag="RD744_002774"
     tRNA            complement(1641..1714)
                     /locus_tag="RD744_002774"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1678..1680),aa:Arg,seq:acg)
     gene            complement(1725..1810)
                     /locus_tag="RD744_002775"
     tRNA            complement(1725..1810)
                     /locus_tag="RD744_002775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1774..1776),aa:Leu,seq:taa)
     gene            complement(1827..1898)
                     /locus_tag="RD744_002776"
     tRNA            complement(1827..1898)
                     /locus_tag="RD744_002776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1864..1866),aa:Gly,seq:gcc)
     gene            complement(1910..1982)
                     /locus_tag="RD744_002777"
     tRNA            complement(1910..1982)
                     /locus_tag="RD744_002777"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1947..1949),aa:Thr,seq:tgt)
     gene            complement(2000..2081)
                     /locus_tag="RD744_002778"
     tRNA            complement(2000..2081)
                     /locus_tag="RD744_002778"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2045..2047),aa:Leu,seq:tag)
     gene            complement(2089..2161)
                     /locus_tag="RD744_002779"
     tRNA            complement(2089..2161)
                     /locus_tag="RD744_002779"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2126..2128),aa:Lys,seq:ttt)
     gene            complement(2165..2237)
                     /locus_tag="RD744_002780"
     tRNA            complement(2165..2237)
                     /locus_tag="RD744_002780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2202..2204),aa:Val,seq:tac)
BASE COUNT          599 a          627 c          536 g          486 t
ORIGIN      
        1 agtatcttcc atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt
       61 cttaaccgct tgaccaacgg gccaataaaa taaatgggcc taaatggact cgaaccatcg
      121 acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat aaaaactaaa
      181 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
      241 actacacccg cataatggcg cgggacagaa tcgaactgcc gacacatgga gcttcaatcc
      301 attgctctac caactgagct accgagccaa acggttccga cgggaatcga acccgcgatc
      361 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccaatgtt tttagttttt
      421 aattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccag
      481 actgctccat cccgcgataa tatgaaaaag gaggataagg gattcgaacc cttgcacggt
      541 tttacccgcc tgacggtttt caagaccgtt cccttcagcc ggacttgggt aatcctccat
      601 gtaaaactat attaaattaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
      661 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaaa caaaatattg aaacggagaa
      721 ggagggattt gaaccctcgc gccggtttcc cgacctacac ccttagcagg ggcgcctctt
      781 cagccacttg agtacttccc caaaaccaat aagattttgt actaatgggc ctaaatggac
      841 tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca
      901 taaaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg aaggctgtgg
      961 ttttaccact aaactacacc cgcatggcgg ttccgacggg aatcgaaccc gcgatctcct
     1021 gcgtgacagg caggcatgtt aacccctaca ccacggaacc agtgttttta gtttttaatt
     1081 gcgggagcag gatttgaacc tacgaccttc gggttatgag cccgacgagc taccagactg
     1141 ctccatcccg cgataatatg aaaaaggagg ataagggatt cgaacccttg cacggtttta
     1201 cccgcctgac ggttttcaag accgttccct tcagccggac ttgggtaatc ctccatatga
     1261 aacttacaac gccacaatgg accttgtagg actcgaacct acgaccggac ggttatgagc
     1321 cgtctgctct aaccaactga gctaaaggtc caggctctta aaaatcgcgg cggaggggat
     1381 cgaacccccg acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgcgaa
     1441 aaagatattc attatttaaa tgaaatggag cctagcggga tcgaaccgct gacctcctgc
     1501 gtgcaaagca ggcgctctcc cagctgagct aaggccccgt gtttaaattt ataattatcg
     1561 ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga
     1621 gctacttccc gtttaattaa cgcgcccaag aggagtcgaa cccctaacct tttgatccgt
     1681 agtcaaacac tctatccagt tgagctatgg gcgcatattt gaaatgccga ggaccggaat
     1741 cgaaccggta cggtgatcac tcaccgcagg attttaagtc ctgtgcgtct gccagttccg
     1801 ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg ttcgaacccg cgacccccac
     1861 cttggcaagg tggtgctcta ccactgagct atttccgcgt gtttaaagat gccggctaaa
     1921 ggacttgaac cctcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
     1981 gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac ccccacgccg taaggcgcta
     2041 gatcctaaat ctagtgcgtc tgccaattcc gccacacccg caaaaatatg agccgtacag
     2101 ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc taccaactga gctaacggct
     2161 catatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
     2221 agctgagcta aacctccatg gataactt
//
LOCUS       DAPCMU010000233         2167 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPCMU010000233 DAPCMU010000000
VERSION     DAPCMU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.241"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..363
                     /locus_tag="RD744_002781"
     CDS             <1..363
                     /locus_tag="RD744_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558714.1"
                     /translation="RPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQET
                     LKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRN
                     FYNFRARIYLQQGLIFEN"
     gene            418..1983
                     /gene="guaA"
                     /locus_tag="RD744_002782"
     CDS             418..1983
                     /gene="guaA"
                     /locus_tag="RD744_002782"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDU0558715.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          668 a          450 c          450 g          599 t
ORIGIN      
        1 cgaccaatta gtcaaacaga aatcgttgat gagctgttaa gctacaacga agaattgcga
       61 actgcgtatc atttttgtca attattacgc tattatttcg ttaaacgagg tacggaaggc
      121 tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa aaagaaattc
      181 acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgtc ccactcaaac
      241 ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc gtttggttat
      301 cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat ctttgaaaac
      361 taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta ttcaaatttg
      421 tcttcaccaa cacgatttga cgaagagcca aaaattatcg tacttgatta cggtagtcag
      481 tataaccaac tgatcactcg ccgtattcgt gagttcggtg ttttctcaga gttgatgagc
      541 caccgcatta ccgcagaaga agtaaaggca atgaacccta aaggaatcat cctttctggc
      601 ggaccaaaca gtgtttatga tgagaaggcc tttggtattg atgaaaagat ttttgatttg
      661 gggatcccag ttttaggaat ctgttacggt atgcagttga ttacttataa attaggcgga
      721 aaagttaaac cagccaaaaa ccgtgaatac ggaaaagcag aattggaaat cacttctgca
      781 gaagcggccc ttttccaaaa cactcctgaa aaacaaaccg tttggatgag tcatggtgac
      841 ctagttaccc aagcaccgac tgatttcgat gtcgttgcta ccagcaaaga ttgccccatc
      901 gcatcgatcc aaaatgcaga acgcaaaatg tacggaatcc aattccatgc agaagttcgc
      961 cattccgaat atggaaatga cttgttgcgc cattttacac tagatgtctg ccaatgtact
     1021 ggtgactgga caatggataa cttcatcgac atggagatcg caaaaattcg tgaacaagtc
     1081 ggtgacaaaa aagttttatt aggcctttca ggcggagtag attcctctgt tgttggtgtt
     1141 cttttacaaa aagcgatcgg cgatcaatta acatgtatct tcgttgatca cgtattgttg
     1201 cgtaaaggtg aagcagaaca agttatggaa agtcttggcg gaaaatttgg cttgaacatt
     1261 ataaaagtcg atgccaaaga acgtttctta tcaaaacttg ctggcgtgtc tgatccagaa
     1321 gaaaaacgta aaatcattgg aaatgaattt gtatatgtct tcgatgatga agcaaccaaa
     1381 cttgccggtg aagaaggcgt ttctttcctt gcacaaggaa ccctttatac cgatgtgatc
     1441 gaatcaggaa ctgaaactgc ccaaaccatc aaatcacatc acaatgtagg tggattacct
     1501 gaagacatgc aattcaagct gatcgagcca ttgaacacat tgttcaaaga cgaagtacgt
     1561 gaattaggta cccaattagg catgcctgac agcatcgttt ggcgccaacc attccctgga
     1621 ccaggcttag gtattcgtgt ccttggtgaa ttgacagaag aaaaattaga aatcgtccgt
     1681 gaatcagacg ctatcttgcg cgaagaaatc gccaatgccg gtttagatcg cgacatctgg
     1741 caatacttca ccgtcctacc aggcattcgc tctgttgggg ttatgggaga tggccgtacg
     1801 tatgactaca ctgtaggtat ccgtgctgtc acttctattg acggcatgac cgctgacttt
     1861 gcccgtatcc cttgggaagt cttgcaaaaa atcagtgtcc gtatcgtaaa tgaagtcgca
     1921 cacgttaacc gtatcgtgta tgatattacc agcaaaccac ctgcaacagt ggagtgggaa
     1981 taaagagcca acataatatg gatgaggaat acctataaat caacattatt caattactaa
     2041 agttcctgat tttcgtaaaa aataagatgg tttcctacca ttttccctac caaagccctt
     2101 gttagattaa ctactaacaa gggctttgct tttattcact acctttattt tcctattgaa
     2161 ttagctt
//
LOCUS       DAPCMU010000234         2116 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPCMU010000234 DAPCMU010000000
VERSION     DAPCMU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.242"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            115..492
                     /locus_tag="RD744_002783"
                     /pseudo
     CDS             115..492
                     /locus_tag="RD744_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            540..1940
                     /gene="yfcC"
                     /locus_tag="RD744_002784"
     CDS             540..1940
                     /gene="yfcC"
                     /locus_tag="RD744_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDU0558716.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1973..>2116
                     /locus_tag="RD744_002785"
     CDS             1973..>2116
                     /locus_tag="RD744_002785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558717.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          587 a          360 c          449 g          720 t
ORIGIN      
        1 gtcattgata tcagtcctat tcgagtatgt tttctgcaaa aaacattgta ctcctaactg
       61 agatcaatgg cttttttgat gcttcacttt tacacaatta tatggacgta atcaaaaaat
      121 gtttccacag aggacttctt taattattgt cgattagagg aaagtggtgt aacagtcact
      181 agcggtacag atttaccgct gtttattact gatattcctg aatcgattat aagagcagta
      241 ttccgtcgtt ttcctgaagg agaagaagtc tgggagaaag aaacaagctt cacgacaacc
      301 gagctgctga aaagttttac aagcaacagc ttcaaagtaa atggtttatc ggagtacgga
      361 gaaataaaag tagggaaaag cgccagctta gctgttttca gcgaggacct ctgtactgat
      421 gactggcaaa aagtgatggc tgcggaagtt atcgctacct atcttgatgg tgacttagtt
      481 tacaagaaat aatgcttttc gatataacgt tataaaacga attaaagaga ggttaaaaaa
      541 tggaaacaat gaaaagaaaa ttcaccttcc cacatagtta tacattgatt tttatcttaa
      601 ttattttggc tgcaatttta acttggataa ttcctagtgg tcagtttgag acagttgttg
      661 aaaacgtcgc aggtatggaa aaaacaacgg ttgttccggg aacctatcac caaatcgata
      721 aggcagatta tggacaaggt ttttcctcta tactaatggc accagctgaa gggattattt
      781 cagctgttga ggttgttgct tttgttctca tcgttggtgg tgccttcggg attatcctaa
      841 aaacgggagc catcgatcga gggctgttta ccttagcaaa agttctggga gacaagggga
      901 tcttagttat tccgatgtcg atgattctat tcagcttagg aggatcaacc tttggcatga
      961 gcgaggaagt cattccctta tttgcggtgt ttatttctct gatgttttct cttggctatg
     1021 attccatgac tgcgatcttg attctatttt taagtacaca agcgggatat gttgggtcga
     1081 cgattaatcc cttcaacgtt ttaatcgcac agggagtagc tggcgtttac ggaaatcccc
     1141 tgctttggtt tcggatgcta tcctggattg tcattactgg gatttcaatt gttttcacca
     1201 tgctttatgc tagcagagtc aaaaagaatc cactgtcctc tattgtttat gagagtgatt
     1261 taaaggttaa gcataagttt caaaattttg acactgaaaa agagttttca acacaagata
     1321 aactaattat cgccggtttt atcgtaggtt taggaatcat gatttggggt atcatttcca
     1381 agggatggta tatggttgaa atttctgcac tattcttagg attaggtctt tttgctgggg
     1441 gcatcgctag attttctcaa aaggaaattg ctgaagcttt tgtcgaaggc tgttcagatt
     1501 tcgcctatgc tgctgtaatc attgggctag ctagaggagt cttggtaatc ttggaaggtg
     1561 gaatgattat tgacactatt ctaaacagct tggctaacct tttaggtggt cttccaaaat
     1621 tcctgttcac tacgattcta ctcatttctc aagtaattgt ttcctttttt gttccatcct
     1681 cctcaggagc tgcggcatta acgatgccgg ttatggcacc attgggtgat ctaatgaatg
     1741 taagtcgtga tgttgttgtt ctaagtaacc agtttggaaa tggtttaatg aatattatct
     1801 ccccaactag tggtgtatta ctggcaggat tagccatcgc agagattaat ttctctaaat
     1861 ggtttaaggt tggcttcaaa atatttgcca tcttaaccct tgtttctgct gttttgttat
     1921 acattgctac actcttttag tcttaatcat ccattaatag gaggcaatag ttatgattaa
     1981 aataaatcaa ttagcaggaa tgaatatcca ctatttattt tattctctca aagaattttt
     2041 cgcttatcaa cagaaaattg gtatttcttc tgttgaactt tggggaggtg ctccgcattt
     2101 tttcatagat gctttt
//
LOCUS       DAPCMU010000235         2103 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPCMU010000235 DAPCMU010000000
VERSION     DAPCMU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.243"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            172..1542
                     /locus_tag="RD744_002786"
     CDS             172..1542
                     /locus_tag="RD744_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDU0558718.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1563..1847
                     /locus_tag="RD744_002787"
     CDS             1563..1847
                     /locus_tag="RD744_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558719.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          729 a          307 c          445 g          622 t
ORIGIN      
        1 ttgttgattt accaacatgt ttagacattt tcctctagga aaaaattaac tagcaccacg
       61 cgtattgttt atattaaatt agatgaccgt gataatgcac agcaaatttt tgaaagtatc
      121 aattctaccg gtgaaagatt gactgcctcg gatttgattc gaaacttcat catgatggat
      181 aaatctaatg aagagcagac tacgctttat actaaatatt ggcgtaggtt ggaagaagtc
      241 tttgataatt ctaaaggaat ggaagacttt ttcagatact accttgctgc aataactggg
      301 gagtattcag caaagcatat tctttatcaa gcattcaaag actattggca taaagagcgc
      361 gatgtatcaa gtgatgccga attgctccaa aaattagtgc gatatgctgg ttattttgcg
      421 agactttact ataacaaacc tgaaggaaaa tactcagaag tattgagcga tttccaaagt
      481 atggaaagta tgatgcctgc accttttgtt ttgggattat ctgaatggta ctatcatgac
      541 caatttatca ctgaagacca atatattgat gcgattaagg tagtgaatga ttatcagctt
      601 aggagatatt ttaatgggga tgatacaagc agagtgtcaa aagcatttcc ttcatatttg
      661 aaaagtgtta ggaagtacgc taaagctaat ggatatgaaa atatcgttga tattgtaatt
      721 tatgttcttg ttactagaaa tcaatcaaat aatatggcac ttccatctga caaggcattg
      781 aaatctaatc tattgaatgc caatgcttat gcgatgagat tggcccgttg gttacttgaa
      841 aaaatagaga atcgagaaaa ttcagcaaca cttgatatga gtaacttgtc cattgaacac
      901 atcatgccac agactagtac ttcatattgg gaagagaagg ctggtacatc gggggaagaa
      961 tacacaggat tagtaaatac cattggtaat ttaacactcg ttacaaatcc tgataattcg
     1021 gccgcgggaa ataaagactt tgagaccaaa aagaaaatct ttgaagatac ccttcatatt
     1081 cgtatgaata aagatttgta cgaattgact gagtggacat catcggatat ttctgccaga
     1141 agtgaagctc taatcaatga actgattact atgtatccct atttgagatc gtcgggcgac
     1201 tatgaacacg atgggaatag ggaaattttc ttagaggcgc aaggaataaa agcaactggg
     1261 tatttgaacg aagacgagac cgtgattata cattcaggtt cagaaattta ttctaagatt
     1321 aaagatattg catctgattc gctagatgaa actcgccaag agctgttgga taatggaatc
     1381 attgaagaaa caattggggg actacaattt gttcaagatt acactgcctc atctgtttcg
     1441 aatgcagcag ctctgctatt agggggtagc cggaatggtt gggactactg gaaatatgat
     1501 aacggaataa gtattaatga ttcgctaaga aataagaaat agagcataga ggtggtgtta
     1561 ggttggataa gaacaaacta agatatgcaa ttcttaaatt gcgtgatgaa aaaaagaatc
     1621 cttttattga gcttaaaggg caagttccag aagaagatat tttagaacaa actaaatttc
     1681 tttcgcgcaa tggcttacta gaatccatgg tttgggcgga taacactgtt catatctggg
     1741 ggagtgtttc tctggaaggg gaaaaatatt tagttgaaaa ctcaggtctt gctaaggcgt
     1801 atgcattggc aaaagaagtt aaaaattgga ttccattttg gggctaagat aagtttttaa
     1861 acggagaagg tagcaaatca taattggttt gttgcctttt ttgattgccc aaaaagaaaa
     1921 atgacaaagt atagagataa atgacatagt acacgggaaa aatgacattg tatcaccgac
     1981 aacgtctaaa tagatgttga tatatatacg ttccgtacaa ataattataa aaatttaaat
     2041 gatttgagta gaagtatcag tgaatatctc aaggggaata gctatctcaa gattcgattt
     2101 tca
//
LOCUS       DAPCMU010000236         2101 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPCMU010000236 DAPCMU010000000
VERSION     DAPCMU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.244"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            715..1047
                     /locus_tag="RD744_002788"
     CDS             715..1047
                     /locus_tag="RD744_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDU0558720.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1666..2007
                     /locus_tag="RD744_002789"
     CDS             1666..2007
                     /locus_tag="RD744_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDU0558721.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          740 a          279 c          435 g          647 t
ORIGIN      
        1 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
       61 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
      121 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
      181 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
      241 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
      301 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
      361 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
      421 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
      481 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
      541 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
      601 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
      661 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
      721 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
      781 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
      841 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
      901 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
      961 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     1021 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     1081 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     1141 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     1201 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     1261 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     1321 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     1381 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     1441 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     1501 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     1561 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     1621 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     1681 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     1741 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     1801 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     1861 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     1921 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     1981 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     2041 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     2101 t
//
LOCUS       DAPCMU010000237         2084 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPCMU010000237 DAPCMU010000000
VERSION     DAPCMU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.246"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            66..923
                     /locus_tag="RD744_002790"
     CDS             66..923
                     /locus_tag="RD744_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDU0558722.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            924..1472
                     /locus_tag="RD744_002791"
     CDS             924..1472
                     /locus_tag="RD744_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558723.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1732..2084
                     /locus_tag="RD744_002792"
                     /pseudo
     CDS             1732..2084
                     /locus_tag="RD744_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          805 a          267 c          442 g          570 t
ORIGIN      
        1 taactaaaaa taaatatgtt ataatagtat caaatgaata ttgctaaaag aaaagaggta
       61 ggaacgtggt agcgaagaaa tatgtaatcg gtaattttaa aggtggcgta ggtaaaagta
      121 cgtgcgcgca aatgttaggg tttgaatcag cagttaatga aggaaaaaga accttgataa
      181 ttgacttgga tatgcaagga aatacttctg atgtgatgaa cttaacgcat atgaacttca
      241 ctaacgaaga aggaggcgga gaaggtgaac cgattgttta cgaaaatact gtgactgacg
      301 tgttgataaa caatcttcca cctaaagagg ctgtctataa agtaatcaat aacctctata
      361 tacttccagc agacatgtcc tttgaaatgt atgatgattg gatcaaagac caatataatg
      421 attcattatc ccaattcaaa tatatgaaag aaaagctaga accattattt gatgagtttg
      481 acgtcatcta tttagatgtc ccaccttcta tttctgtata tagtaaatcc gcgatgtaca
      541 ttgcggattg ggcaatcgtc gtcttacaga cccaagtaaa atcgatgaga aatgcgatgc
      601 aatatcttga gtatatggac ttttttgtga aagaatttga tacaaattta tatgttgctg
      661 gtgttattcc tttcatgttg gaaagcggtg acgcagtaga tcaggaaatg tataaacaag
      721 cccaagagat ttatggggaa catttgatta aaaatgttgt actcaaaaat gcgcgcttaa
      781 aacgatacga cggatcaggt attacgatgg agaagacaaa gaaaggtctc ttaaaacaat
      841 gggatcgcaa aagccacgaa ttatttatca gtattttgaa agaattggaa gaacacgaga
      901 attggtatgc ggaggtggag taattggctg acatcaaagg acttgatatt tttgggaaca
      961 acaaaggact cgccaaaata aaaaaagaag cagaagcaaa gaaaaagcag caagcaatta
     1021 caccatctga aaataagcaa ttagaagtgc accaagttca agaaataaaa acttcctcca
     1081 atgttgaaag acagagtaga ggaagaacaa aaaaagttgg cgcaccaatc agaaagtttg
     1141 ataaggttta ttccaccaag catcctttaa aactgtcgac tttgttaaat gcgacttcca
     1201 gagttttagt agaaaagtat gagactaata tgacaagaga tgaattgctt agaaaagctt
     1261 tagatgagta tattaaacag aatcttacgc aagaagataa gcaagatttg tttaatgacg
     1321 tggtgaaaga actagacttg ttcagagaaa aatatccaat tgttccagaa ttagatgatc
     1381 gtggagaaat aataagaact accagtcaga taaaacaaga taccgaagac tcactgcgca
     1441 aagggtgggg aatgaataat aaagttagat aataatcaaa gaagaactaa gtaactacct
     1501 ttaaacggta gttacttagt tcttctttga tttgcgtgaa atagagggga tttatttttc
     1561 tggttacgtg attaaaaaat ccaaaaaatg agcataggaa cggttatgat tttagggtgt
     1621 gttttaatga gatattgtga tttcattaac aagtgtatga taatctgttg ttgagttagt
     1681 aaataagtaa tagagctaac tctaaaataa aactattagg aggagatgta gatgaaaaag
     1741 tttaaaaaga aaatatattt aggattggtt gttggggtga gtatgtttgg gttcatggct
     1801 catactaaac aagtaactgc agatactaac caaaaagaaa ggattatgag ccaattagat
     1861 gactctcaaa taagtatgta cccaatgagc taattgtcag tttaaaggct acacaaagaa
     1921 tttcaacaga gaaaaataaa ccgcaaaatt tattgggtgt aaaagttatg gtggaaaaag
     1981 atttgactct taggataaat gaaagcgatt ataataagcg agatcaagta ttattagtga
     2041 aattgaacga agaaattagt gtggaagagg cagtaagaac attt
//
LOCUS       DAPCMU010000238         2081 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPCMU010000238 DAPCMU010000000
VERSION     DAPCMU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.247"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..153
                     /locus_tag="RD744_002793"
     CDS             <1..153
                     /locus_tag="RD744_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDU0558724.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="RD744_002794"
     CDS             159..575
                     /locus_tag="RD744_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558725.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="RD744_002795"
     CDS             681..944
                     /locus_tag="RD744_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0558726.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="RD744_002796"
     CDS             944..1216
                     /locus_tag="RD744_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDU0558727.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="RD744_002797"
     CDS             1293..1622
                     /locus_tag="RD744_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDU0558728.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1612..1898
                     /locus_tag="RD744_002798"
                     /pseudo
     CDS             1612..1898
                     /locus_tag="RD744_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          777 a          264 c          403 g          637 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa gctaaaagag aagccattga
     1681 aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat aagggtgaac
     1741 aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg ggcacaacat
     1801 atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta tgtggaacgc
     1861 atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc gttcttaaga
     1921 aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt cgcgagtatt
     1981 ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata aatcaatata
     2041 tttcagcttt tccttattta tgtactacct tttacttatt a
//
LOCUS       DAPCMU010000239         2073 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPCMU010000239 DAPCMU010000000
VERSION     DAPCMU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.248"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>2073
                     /gene="acm"
                     /locus_tag="RD744_002799"
     CDS             <1..>2073
                     /gene="acm"
                     /locus_tag="RD744_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDU0558729.1"
                     /translation="TNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFS
                     FDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNE
                     NIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFY
                     YKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQ
                     KVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQI
                     NFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIP
                     NVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSS
                     NELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEP
                     VPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLV
                     VTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFT
                     NLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPE
                     KTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSS
                     FAVIIGLL"
BASE COUNT          813 a          342 c          405 g          513 t
ORIGIN      
        1 ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca
       61 gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa
      121 aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga
      181 tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa
      241 tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt
      301 ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg
      361 ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg
      421 ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt
      481 attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata
      541 ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg
      601 gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg
      661 tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta
      721 atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta
      781 ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga
      841 atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca
      901 atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat
      961 tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat
     1021 taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg
     1081 aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag
     1141 aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa
     1201 acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca
     1261 tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat
     1321 tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag
     1381 acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg
     1441 aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt
     1501 atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct
     1561 caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca
     1621 cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg
     1681 attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata
     1741 ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa
     1801 acagttatac accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga
     1861 aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc
     1921 aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag
     1981 ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa
     2041 aatctagttt tgctgtaatc attggtttat tga
//
LOCUS       DAPCMU010000240         2065 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPCMU010000240 DAPCMU010000000
VERSION     DAPCMU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.249"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1008
                     /locus_tag="RD744_002800"
     CDS             <1..1008
                     /locus_tag="RD744_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558730.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="RD744_002801"
     CDS             1025..1174
                     /locus_tag="RD744_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558731.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="RD744_002802"
     CDS             1171..1617
                     /locus_tag="RD744_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558732.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="RD744_002803"
     CDS             1619..1756
                     /locus_tag="RD744_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDU0558733.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="RD744_002804"
     CDS             1794..>2065
                     /locus_tag="RD744_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558734.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAPCMU010000241         2031 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPCMU010000241 DAPCMU010000000
VERSION     DAPCMU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.250"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            142..756
                     /locus_tag="RD744_002805"
     CDS             142..756
                     /locus_tag="RD744_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558735.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(921..1745)
                     /locus_tag="RD744_002806"
     CDS             complement(921..1745)
                     /locus_tag="RD744_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558736.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1769..1924)
                     /locus_tag="RD744_002807"
     CDS             complement(1769..1924)
                     /locus_tag="RD744_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558737.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT          677 a          336 c          294 g          724 t
ORIGIN      
        1 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
       61 tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
      121 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
      181 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
      241 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
      301 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
      361 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
      421 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
      481 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
      541 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
      601 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
      661 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
      721 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
      781 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
      841 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
      901 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
      961 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     1021 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     1081 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     1141 aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt ttttagcact
     1201 gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc
     1261 aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca
     1321 atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa
     1381 ttctcaaata ttagagttaa atcaggtttc tcttgttgat aaaattcctt tacccctatt
     1441 tcacaggctg taattatcga aataaaccta tctctagtat ttgatatatt tttacttctt
     1501 ttaaggagta taagagaaaa ggggatactt tcgtgtccat gcatgtaatt aactaattgt
     1561 tgagcttcag aagttatatc tctaattata tagttaacgg aattaggaag tgtctttaac
     1621 tctaatgaaa tttctatcca attactatct tctaatctgt atatgctcga agtataattt
     1681 gattcgtcag caagcatcgc tacatcccaa acactgatca taaaatcttt taaacgttca
     1741 atcatttcag caatattgct aaaatcagct aatttttctt tagaataaaa aaaacattta
     1801 tatcagaatc aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat
     1861 ttatgtttat tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt
     1921 ccattacttc tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa
     1981 taaatcatta atttttagct aaattctatg agaagtttat ttttgttgtt t
//
LOCUS       DAPCMU010000242         2011 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPCMU010000242 DAPCMU010000000
VERSION     DAPCMU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.251"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..914)
                     /locus_tag="RD744_002808"
     CDS             complement(<1..914)
                     /locus_tag="RD744_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDU0558738.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="RD744_002809"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="RD744_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDU0558739.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          665 a          368 c          394 g          584 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa a
//
LOCUS       DAPCMU010000243         1988 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPCMU010000243 DAPCMU010000000
VERSION     DAPCMU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.252"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            121..543
                     /locus_tag="RD744_002810"
     CDS             121..543
                     /locus_tag="RD744_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558740.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(709..1902)
                     /locus_tag="RD744_002811"
     CDS             complement(709..1902)
                     /locus_tag="RD744_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0558741.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT          717 a          410 c          334 g          527 t
ORIGIN      
        1 cttaggaaaa aaagagaaat aataacaggt ttatgcacta ttgacggtgt gacaatatga
       61 tatttgtatc cgtttttttc ttgtatgtag tatagtaaag tagatacagg aggagaaaat
      121 atgcagactt ttttaacaaa agtttctcaa aagaaaatta aaatgattca tttactgctt
      181 gaaacagaac gttggtgttc aatagaagaa ctacaaaacg aattgaaggt atcaagtaag
      241 tctatcttga attatctaac agatttagaa gaattgttcc aacaatatcc tgataaggtc
      301 attttgaaga atgagaataa tcggcgcttt tctatggaaa agcaggagaa cttcccaatc
      361 tacattattt atcttcattt ctacagaaaa tcatataatt atcatttgat tgaattcatg
      421 tatcaacatc ctgagaaaaa tttggaagat tatgcagacg ctcaatatac gagtgtatcc
      481 acagtttttc gttatgctaa gttattagta aagtactttg aaatgacgga cgtcaaagaa
      541 tagtcgccgc cagatagaag gaaattagca gttgaaaaag ctgccctgca tcaagtaata
      601 cgaccagcca aacggaaaca gtgtctcatg tttttgattt ggctggtctt attaccgcag
      661 atgcaagctt tttccacacc tctttcttgt catttacaga ctcaactatc aatgctgcaa
      721 gctttccctt aatgctgtaa caccagtaag agttgtcaag accagcagac tgactaggta
      781 aataccgctc aaaaacaaga ttacggcagt taaactataa tgatccatca agaaaccgat
      841 gacgatagaa gaaaaaccac caacagctcg tccaaggttc ataacgaaat tattcgccgt
      901 cgcgcggatt tctgttggat acaaactact aatcactgcg ccatatccac catacatgcc
      961 attgataaag tagccgacta tcacagcggc tgctaccaaa ctccactggc tatgggctaa
     1021 cacaagtaga tagacagata aggatgaaca aattaaaaac aaagaaaagg ctagtttagg
     1081 ccccaattta tccatgatta tgccaaaagt catcatcccc aaagacatgc ctaaaatagt
     1141 actgaccatc cacaatgaag acccagaaac cgaaagccct aatcgatttt gcataattga
     1201 aggtagccaa ttcattaacc caaaatagcc ggcaatttgg acagttacca tgatactaag
     1261 gccacatgtt tgccaagcaa gatgttttga ttcaaacagt ctgctaagtt tggttttctt
     1321 ttcggcagat tgtacagatt tgaattcctt tggttcagtc aagccgcgac gaatgaacaa
     1381 gaccatcaac accggcagta cacctacgac atacaacatc ttccaaccaa acgttggaat
     1441 gatcacagca gctaaaatcg cagccaaagc cgcaccgacc tgcgcaccaa taccagcaac
     1501 cgaagaagct cgtccaattt gtttttttga aaaactttcg gaaatcaagg acatacacgc
     1561 cccatattct cctcccgctc ctacacctgc gataaagcga aagagataaa ccaagtagat
     1621 gtttgaagca aaatacatca ataaagaagc aatcgaaaac agaaggaccg tttgagaaaa
     1681 tactttcaca cgtccatatt tatcagctat gatgccaaaa aaaatacctc caatgagcat
     1741 ccccaaatta gtgatcgttg aaatgaaacc cgcttgcgta ccactaatat gaaaagaagt
     1801 aatcattgaa gataatgaaa aagctaaaaa cataatatcc atattctcta aagcaattcc
     1861 cgccgatgtc gatactaata cctttcgctg atacgttgtc atttttatac cccttttcct
     1921 aaaatttccc taaattgtta aaagtatatc caaaaaagaa taaaaaaaca agactttttt
     1981 atttgaaa
//
LOCUS       DAPCMU010000244         1979 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPCMU010000244 DAPCMU010000000
VERSION     DAPCMU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.253"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            54..947
                     /locus_tag="RD744_002812"
     CDS             54..947
                     /locus_tag="RD744_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDU0558742.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1081..1206
                     /locus_tag="RD744_002813"
     CDS             1081..1206
                     /locus_tag="RD744_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558743.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1340..1972)
                     /locus_tag="RD744_002814"
     CDS             complement(1340..1972)
                     /locus_tag="RD744_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558744.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          763 a          295 c          368 g          553 t
ORIGIN      
        1 ttttacgtaa aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact
       61 attattcaaa aaataaggaa aacttttatc aaaaactgac cgatgatccg cttttttctt
      121 cattgaccga ttatctatat gaacatcgag aacaagaaac gattctaaga gaattgaaaa
      181 aagaattttc gcaaaataaa ttttcccatt ttcttgattt attgatcgat gcaggattaa
      241 tcaagaggga agaacgaaga tatcatttga actttccaat atttgatcct aaagattact
      301 tgcaacaagc cacttcggct gctgaaacca tagctgaaca attgaagaga ctctctgttg
      361 atgaacaaaa attagcgatg ggagaagtta tttgggcgta ttgtttcgaa gatgaacgga
      421 aagaagcgta tttttatgga gtaagaaaca gccgagagac agaattgctg aggacaactg
      481 caggaaatga gaagtaccgt tttatcacac tttcctctat agaacacttc ccgcttactt
      541 tggctaatta cttttttgta cagaagaatc agcttcctgt aacgaaggca ttcaaagaat
      601 tagccgaact tattggagac gtaaatgaat cgtacttttt tgatcaaata gaagttatcg
      661 ttgacaggat acgtaagaat aaatataaaa atcgccggcc tagtattttc catcagtctc
      721 tacttgtgac gaatactata aaggaagaag aatcattcac attagaatta cctattgtcg
      781 aaaaaaacaa ttttgagatt gaattaccaa cattggatcc ttcattgaca atggaggaaa
      841 tggcctttct taaacgacaa atcttttctg agctgagcaa gaaatttata ccgcatgcat
      901 tttcttatat caaagaatat ggaacagttt tagtgagtaa aacctaagag agcagtaagc
      961 aaaaattata ctgactcttg caggaggagc ctttctcttc ctttatggtt aaaagaatta
     1021 tttgagtatt ttgcctatct accacctatt agattcacta catggtctgg aaacaaagga
     1081 atgcaaaaaa atggtgagaa atgtggaatg acaaaggaag tagtgataag aaaagttaga
     1141 tttttaaata atcaatatta tgattcagtc aagtatggca ttttatggga agaactagct
     1201 gattagatta ggaagtccat ttgataatcc agaatcacta agaaacgaat ggcgagtcat
     1261 taataattta tccaatcttg ggttattctt ttagattgaa gtgtttaaaa ctaaaaaagc
     1321 atttatgaag tcgtaatagt caggcttcaa aatgcttttt attttgtttg ttttttcttt
     1381 gccacataaa tacacctaga ctgacgatga agagcatatc caacagattt cctgcgactt
     1441 ggatcgtcga agggttggtc agtggcatta aagcaaggac aaaaaaggaa aacaagctgt
     1501 aaataaaaca ggtgacagga actaatcgaa agaagagcgg acatttcaat tggctaaaaa
     1561 tgagtgaaaa aataaaataa gtaaaaatgg ataaaatcac gactaaacca ttcatgataa
     1621 agaagaaaag cgtattgtga ttaaacgaat aaaaaagaat tgctaaaaaa gaaaacgttt
     1681 taaatgcaat caatacgctt gaagcttcgt ttaacgtacc aggatgtcga tgaaaaaaat
     1741 aggcaccgaa ataaacagaa aacagtaagc cgcaaagaga agacaaaatc aaaaaaaacg
     1801 gattttcttc ggcttctgtg atatcagaat gatccaaaag caaccagcct aatgcactag
     1861 aaggcgcatg tgaggtctta aggtatgaat aacatagaaa aaataagatc gtcaaagttg
     1921 caaaaaaaga gcctaaatgt aaaaaattag tggttctacg ttgaatttcc ataatatct
//
LOCUS       DAPCMU010000245         1962 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPCMU010000245 DAPCMU010000000
VERSION     DAPCMU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.254"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="RD744_002815"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="RD744_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDU0558745.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="RD744_002816"
     CDS             853..1041
                     /locus_tag="RD744_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDU0558746.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="RD744_002817"
     CDS             1054..1611
                     /locus_tag="RD744_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDU0558747.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="RD744_002818"
     CDS             1643..1885
                     /locus_tag="RD744_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDU0558748.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          305 c          404 g          537 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt tt
//
LOCUS       DAPCMU010000246         1929 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPCMU010000246 DAPCMU010000000
VERSION     DAPCMU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.255"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            33..1372
                     /locus_tag="RD744_002819"
     CDS             join(33..474,474..1372)
                     /locus_tag="RD744_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDU0558749.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYRVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          745 a          291 c          320 g          573 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattacaggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttcaagaac cgggggcttt attcctgcag tccggctacg ccttcctgca
     1441 atagacaaaa ctccagatcg ggtaattcgc ttgtggcgaa acccgacctg gagtttgtct
     1501 ctgtattttg aaccctaaaa attaatcaaa cagactgata ttctcataat cagcgcgaaa
     1561 taagccatta agtacagttt tcctacctat ttttgataac ccgctggtta gttaatattc
     1621 cttttccgag agagataaat gattggttaa atctatatac atctcaacct ttaaaaatgt
     1681 tttcgaaata cttcatcagt acatgtcata caaattacca gtgcattctc aaattctagt
     1741 tctttaattg atttcagttg aggagcagtt acttttggcg gaaatttcca tttttgtcca
     1801 aacctcttcc cccgatgaaa tttctttgtt gcaattattg caatgaaaaa tttttgtttc
     1861 aaaaatatac ataattactt cctccaataa aaactatcta cctctcaaat tatttttctc
     1921 taataagaa
//
LOCUS       DAPCMU010000247         1892 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPCMU010000247 DAPCMU010000000
VERSION     DAPCMU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.256"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(336..791)
                     /gene="tnpA"
                     /locus_tag="RD744_002820"
     CDS             complement(336..791)
                     /gene="tnpA"
                     /locus_tag="RD744_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDU0558750.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1034..1892
                     /locus_tag="RD744_002821"
                     /pseudo
     CDS             1034..1892
                     /locus_tag="RD744_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          600 a          368 c          355 g          569 t
ORIGIN      
        1 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat ccagtgattt tctaggaaga
       61 ttatttctgc gccagcattt tctaatgttt gaatttgtgt tttgtaatct tggttgcttg
      121 tgctaattct tgaatagccg taaattgcca aaatttcatc accctttctg gacataagtt
      181 tctgacacca gttaaaatca agtctatcag taacccatac acccgtgagg acgggtgtat
      241 ttttaatccc cctagaaggg gtcgacactc gctgcccctt aaaaggggat ttttaaaaga
      301 ccgccaactg ctcatcttca ctcacttact tctctttatt ttttaaatgg ttctacgtac
      361 tctcttttac ttataatata tcgaatgcga tcatcagctt cttgttcacg gatgtatttt
      421 tcatttgtct tttgattcaa tccgactgta ctcacatagt atctcttcga ccaaaaggat
      481 ttgtttccgt atttatattt gagatttgcg tgctgctcgt ggattagtac cgcactacga
      541 cctttcagat accccataaa actggatata catatttttg gtggtactcg aacaagcatg
      601 tgaatatgat ctagcattgc atgtgcttct atgatttcca catctttcat ttcgcacaac
      661 ttcctaagta tttttccaag atcttttcgt aattgcccat agattacttt tcgttgatat
      721 tttggtgtaa aaataatatg atacttacaa ttccaacgag tatatgctaa acttttatcg
      781 tcatttgaca tgaaaaaact cctttcgatc tgatgagcgg ttggtagcca ctcactagta
      841 tatcgaaagg agtttttatc gctaaagata cttcttccac gcgtagaaac gcgcggtttt
      901 cttgtttcgc acgctttacg cacaaaactg actgaagtca ttaagaaaat aaaagggtct
      961 aaaactttta agttttgtac cgacccccaa aagttagacc aaaaaatcta acttttggag
     1021 gtcggtattt tttatggcta agtatacttt tgaacttaaa ttaaagatcg ttcatgatta
     1081 cttggatggt aaaggcggaa gtgattatct ggcaaagaaa tattccatta aggctccatc
     1141 acaagtgaag cgctggatca atgcctatca agaatttggt gaagagggtc tagtaagaaa
     1201 gcgacaaaag aaaaaatatt ctgttcaatt taagctggat gcgatagagt tatatctaac
     1261 aagtgagctg tcctatcgtg aagttgccaa cacacttaac atgaacaacc ctaatttgat
     1321 cgctagttgg atgcgtcaat tccgtgaggg tgggatcgat ggactctcaa aaacgaaagg
     1381 atgtccacct atcttgtcta agaaaaatga acccaaaaat aagaaaaaat ctacaactaa
     1441 agcaacgtct aaagaacgtg aacgcatcga ggagttggag aaacgagtcc gttcccttca
     1501 aattgaaaat gcctttttaa aagaattgag gaagctgcga aaacaggaag cccaacaacg
     1561 acgaatgaag caatcgcacg aatcatcgca agcctccgag gaccgtttaa attagttgag
     1621 cttctagaaa ctttaaagtt ccctaaggca atctttatgt actggcaaaa acgattggac
     1681 cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aagttcggga gaagcacaag
     1741 gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa
     1801 aagaaagttc agcgcctgat caagaagcta ggaataaaag taacgacata cacacgtaaa
     1861 tcacggcgct acaattctta tcgaggacaa gt
//
LOCUS       DAPCMU010000248         1832 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPCMU010000248 DAPCMU010000000
VERSION     DAPCMU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.257"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            343..1161
                     /locus_tag="RD744_002822"
     CDS             343..1161
                     /locus_tag="RD744_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDU0558751.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1234..1593)
                     /locus_tag="RD744_002823"
     CDS             complement(1234..1593)
                     /locus_tag="RD744_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0558752.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          607 a          282 c          342 g          601 t
ORIGIN      
        1 gttatgaatt atccagatca aatttgtgta ttacattcag taataaggtg cttatcttag
       61 gatcaactta tgatactagc tgaccaagga tgtaccagtt atagaaaatc agttaaagta
      121 aaaatgataa aaatcttatg tcaacactga tttaggctga cctgtgggtt aggagaaaga
      181 caggtcggta gcttttaata caaaaaatgg cttgtatgta tctagatagt taactgaaat
      241 tagaaatgtt cttattttta acggagtaag aaaaataggc tgctttaggt ctgaatactt
      301 tttcaatata atgaagatat caaaggcgac aggagtgttt ttatgaataa gaatcctcat
      361 aatctaaagt tggtagcagt agatatggat ggaacatttg ctcgtactga ttacacatac
      421 gatatcccaa ggttcaaggc tgttttatcg cgaatgaaag ctgttgattg tcaatttgtt
      481 gttgcaagtg gaaatcaata ctaccaattg agatcgttat ttccaaatta ttatgatgaa
      541 ttaagttttg ttgctgaaaa tggtgctttt gttaaagata aaacagaggt actattttca
      601 gccgatatgc ctagaaaagc gatcgaggtg gtccttgatt tatgtaaaga ctatcctgaa
      661 gtaaaaaatg tgatgtgtgg tctgaatagt gcctactgtg aaagaggaaa ggtgagtcaa
      721 gcattttttg atctaaccaa tatctattac catagattag aatgggtagc tgattttgca
      781 gaagttcaag ataaaatttt aaaatttgct ccgactatgc ctgttgaaaa aaccgaattt
      841 tacgttgaat tgttccgtga aaaattagcc gggctattag tacctaccag tagtggtcat
      901 ggttctgtag acttgattct ttctgagtgc cataaaactt tcggcttgaa aatgttagtt
      961 gagcgcttgg ggattaatcc tgaccagtgc gttgcttttg gagatggggg caatgacatt
     1021 gagatgttgg agtattgtgg tctgagttat gaaatggata atgcaacgga agctgttaag
     1081 caagttgcta agcatcaatg tccttcaaat gacgaagacg gtgttttagt gacgctcgat
     1141 cgattgttcc ctaacacgta aacactaggt aattcatcaa aaaacctcga tataagaaat
     1201 attcgaggtt ttaaaactat atttagaaca gatttacgaa gcgtctgatg agtcaaaata
     1261 gacttggaca ttgctatcta aaataccact ttcattcttt aaacgaattg tttgatttga
     1321 tggagttacc tctaatacta aatgcccatt aataaaggta gtatcgtatt cagatgtttt
     1381 aactgttgct tcttcgcaag gtattgttaa atcatatgct ggaatataat gttgtgtgga
     1441 aactaataaa aaatgatcac caatcataca gcatccctct gctgaagaaa attgattaga
     1501 gccatcattt aaagttaaca agaatccatc ctttaaacga ggtacttgct taaatacttc
     1561 tggttgtact tctaaaaata aattatcgct cattttcacg accaccatcg acttccacag
     1621 ttgaaattga cattttattt aacacaccta ttccccctta actataactc aattctatat
     1681 aaaaaatatt ctattctcaa ataattgaac ttttcaaatc gccaatttac ttcggcttta
     1741 tcagagctca ttcatatcaa gagcgattaa cgctgtaaaa attaaaccga agatctgata
     1801 aattttctct attttctaca atatttaaaa cg
//
LOCUS       DAPCMU010000249         1796 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPCMU010000249 DAPCMU010000000
VERSION     DAPCMU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.258"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="RD744_002824"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="RD744_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDU0558753.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAPCMU010000250         1791 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPCMU010000250 DAPCMU010000000
VERSION     DAPCMU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.259"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            88..1701
                     /locus_tag="RD744_002825"
     CDS             88..1701
                     /locus_tag="RD744_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDU0558754.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          567 a          325 c          380 g          519 t
ORIGIN      
        1 tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac
       61 gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc
      121 ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat
      181 gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg
      241 atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac
      301 atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca
      361 gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct
      421 tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt
      481 gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca
      541 caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag
      601 catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat
      661 ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta
      721 gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc
      781 gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg
      841 aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa
      901 accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact
      961 ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca
     1021 gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa
     1081 atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc
     1141 ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa
     1201 gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga
     1261 caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc
     1321 ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct
     1381 actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc
     1441 gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta
     1501 gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca
     1561 atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact
     1621 ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa
     1681 gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat
     1741 ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta t
//
LOCUS       DAPCMU010000251         1777 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPCMU010000251 DAPCMU010000000
VERSION     DAPCMU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.260"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            79..1776
                     /locus_tag="RD744_002826"
     CDS             79..1776
                     /locus_tag="RD744_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558755.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          575 a          334 c          300 g          568 t
ORIGIN      
        1 attatattat cattgttatt tttaacagga attgcttatt tcttttattt tattttttct
       61 cctgcggaca gcaatcaaat gaaagaaata aaagattcgt gggttttcta tacaaaagat
      121 gatcctgatt ttaagtttga cagcaagtac actcgtacga ttcctaccgt ggataaagac
      181 gaaacattca ttatggaaac gacacttgaa aaaccgttgg acgaagcaaa tttactaatc
      241 aaaggaaacc atcaatggat tactgtatat ctaggcgaaa agattttata ccaacggaaa
      301 gattatcagg aagaaagcaa tcccggactc tcattggctg tgatagattt accagcaaat
      361 tatatcggaa aaaaattgac ccttacagtc tcatctcctt atcagaacta tgctggactg
      421 ccaccgaaag tttatctagg aacaactgga ccattgatta gttttattta ttcacaatca
      481 gtgccgcaag tattgacgat gataatcgct gtttttatcg ctatagggac ctttttctat
      541 acgatctact tgatgtataa acaaaaacgt cttgaatact cacttattct cttaagttgc
      601 tttgctttgg cattaggatt tgaagctgta tcagaagata ttttaagtgg tattcttttt
      661 gaaccattcg ttcattcttt tttatctcat ttatttatta tcttaacgtc agctttcatc
      721 atctgctact attggagccg aatgttttac tataaaaaat ggtacggaat tttttgtacg
      781 ttccaacttt tgatttttat cggcgttctg ctttatgcaa cttttactcc tgcagaactt
      841 cctgaattaa tgccgattgc taatattgct agtgttataa gcactttagt cacttcactt
      901 gcatgcattg gcgaagcata taagaataac cgtttttatg tagcttgtac accttggatc
      961 gtcttggtag ccattatcca ttgcttgatt tacatccaaa cggcattagg tatctatcaa
     1021 acaacgatca attggtctac gattttatat attatcattc taatcgttat tatcagctac
     1081 aatctaatcg atcacatctt aaataccgac aaagatcgga gacaagtaga tttcttaaaa
     1141 atcaaaaatg atttgttgga gcaacattat gagcagcttc gacagcatat ccaagaagta
     1201 aatacacttc gcctggaatt tgtccaagag atggagaatc tgcaagattt aatgcatacg
     1261 gaccaaacaa aagctaaaaa ctatgtggaa aagatattag aagaagcaaa aaatttcgaa
     1321 atgctgtgtt ctttttgcaa ccaccaggtg acaaatctta tcttagcgcg ttaccagaag
     1381 ttagctgcca aacggaatat tcagataacc ttccaagcag atcttcccga agaacttcct
     1441 atcaacgatg acgatctggc tcaacttttg atccacgctt tagagcattc ctttagagaa
     1501 actcatgcca tcgaaaatcc actgtatcga aaaatttatt tgtcgataca cgaacaagag
     1561 gaacatttcg tcatttgttg cgaacatagt gcacattacg atacaaacct atttagtcga
     1621 ggcatcacag aagaattaga tgatcaggaa cgttttgact taatgatgat gaaagacgta
     1681 gcggatcgtt acacaggaac actaacgcag gaaaaagata ctttcattga tcggatcact
     1741 gttcaactct ctcgaaatta cccgaatcct gtataaa
//
LOCUS       DAPCMU010000252         1720 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPCMU010000252 DAPCMU010000000
VERSION     DAPCMU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.261"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(28..1509)
                     /locus_tag="RD744_002827"
     CDS             complement(28..1509)
                     /locus_tag="RD744_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558756.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          532 a          232 c          299 g          657 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
     1501 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
     1561 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
     1621 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
     1681 aagatattct tgtttttaag gcggaaaaat tgatttttaa
//
LOCUS       DAPCMU010000253         1677 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPCMU010000253 DAPCMU010000000
VERSION     DAPCMU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.262"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            57..668
                     /locus_tag="RD744_002828"
     CDS             57..668
                     /locus_tag="RD744_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDU0558757.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            850..1365
                     /locus_tag="RD744_002829"
     CDS             850..1365
                     /locus_tag="RD744_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HDU0558758.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(1500..>1677)
                     /locus_tag="RD744_002830"
     CDS             complement(1500..>1677)
                     /locus_tag="RD744_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDU0558759.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT          544 a          230 c          326 g          577 t
ORIGIN      
        1 acttttagta aggagaatta tgagcaaata aacatttgct catagagaga tagagaatga
       61 tagatattat ttctgctata acggtaaatg tattaacagc attatatcag ccgttcttat
      121 ttgccgtatt aacagcgtat ctgtttatgt actggtatct atttatagtt gataactctc
      181 gagatcctaa aaacttaaaa gattctgtat taatctggtt gagatatttt aaagcatcta
      241 gtaaatttcg cagactcttt tttcttgcgt tttattttac tttgatatta tttaaaacct
      301 tgcttaatcg aaatatgtgg atgaacccgc tttcaaacgt tattggacag tggtcattat
      361 atattataga taatgctaca ggagagagca gattgacaac agaatgcgtg gagaacacga
      421 tgctgtttat ccctttttgc atgttattgt tatggtggcg tgacattaaa tgtgatatta
      481 tacgcaccat ctatgttggg attaagtatg tgtttttatt ctcattaagt atagaattta
      541 cacaactgtt ttttaggctt ggtacatttc aactatcgga tttgttttat aacaccctag
      601 ggggcttaat tggtgctttg ctatattggc tattctaccg attaaataag tatatagact
      661 taaaatagaa ctgctttgaa ttttaatatg gttaatattt ttctgcagac aggattgtgt
      721 aatctcatca agaatacttt acatgccatc tatttttgct aaaacattgt tgttatatct
      781 tcaagaggta gggaatttta ttgctttaaa tgaggtcgag cgaattaaaa aattggtaaa
      841 atatataaca tggtggtagt tatggtaaaa gtgtttgata taattaagtt ttcggatttc
      901 agaggatata ctgctgttgc attggataat gagactcttt tctcgattgg tattgagttt
      961 cacattaagc aaataaatca aggatttagt cataataaat atactatgcg agggctacat
     1021 tatcagagtg gagagcatgc tcaaggaaag atagtgatgt gcctacatgg tgaaattatg
     1081 aatatcgcac ttgatattcg acaaggatct tccacttatg gatgtgtgta tgtcaacaca
     1141 ttgagtgaaa gcaatggtaa atgtgcgtat atcccacgtg gatatgctca tggctatctt
     1201 actatggagg acaatacgct agttcagtgg tgcgtagata atgatttcaa ttctaatgct
     1261 gccaaatgta taagttatga aagcgtagat attgaatggc cggtcagtcg gcagtgtttc
     1321 gttataagtg agaaagacaa aaatgtggaa agattaagct tttgattaaa atgagctgtg
     1381 gagaaaagaa ggatactgga aaataagaag aacaataacc gagcaaatgc tcggttattt
     1441 ctatgaggaa tacataaaac taaatttaga tatagatata cccgtggtgc tagttaattt
     1501 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     1561 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     1621 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgttttt
//
LOCUS       DAPCMU010000254         1677 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPCMU010000254 DAPCMU010000000
VERSION     DAPCMU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.263"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(286..569)
                     /locus_tag="RD744_002831"
                     /pseudo
     CDS             complement(286..569)
                     /locus_tag="RD744_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(590..1441)
                     /locus_tag="RD744_002832"
     CDS             complement(590..1441)
                     /locus_tag="RD744_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558760.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            complement(1546..>1677)
                     /locus_tag="RD744_002833"
     CDS             complement(1546..>1677)
                     /locus_tag="RD744_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558761.1"
                     /translation="NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          494 a          329 c          230 g          624 t
ORIGIN      
        1 tcgtcacatc ttcgatcaat ttctcttacg attttctcat ccacatttct tattcttact
       61 tccatattaa ttcctccaat ttaaaaagga tagcgaaaag gagcctatcc tttttgctat
      121 cctactaccc tccttttact atccttcact atcctttcac tatcctattt ttaaatattt
      181 tgttcccaca tttatttttg agaaacgtaa taggcacagc ctattggaac acttttttta
      241 aaaagtgttc ccacgtttcc ttatgctctt tacaaataaa ggcttttaga gacgttctgg
      301 aattttagat tccttgaatt gttcccattc atcaatcaat tggctataag tagccattaa
      361 tttttttctt gctcttgttg ctttcgaatc aacaaaaaat cagtttgatt cgttaattct
      421 aaatattctg ttcggtattc aatgttcatc gtacccgttt tactacatag taactgatta
      481 tgacgttctt tcactttgaa taactgctcc ttctttaagg tttttaactc catgccaact
      541 ataagcacaa tcctgtatag aggaatcatt ttcaaacgat tctccataat tatttatctt
      601 tcattggaag ttctaaatca ctactgttcc aaaaaatgta tggtttgttt tgacgaatat
      661 tacttccatc taaccacaat tttccgatgc catatcgtag gcttgcttcg acttcataaa
      721 aattgtagat ctcttttctt cgatctattg ctttcatttc ctggccattt tttaaaatag
      781 atctgatgcc cttaacagta gattcatgga tactcgtgtg aacgatatcc gtattgatca
      841 attcgtcata tcttccaaaa tcaacattgt actcataacc aagaagtgtc ggaatcaggt
      901 caatttcaat ttctttttca attgaacgtt taataagttt cgtggataca tacccgacaa
      961 aaaagagaat aatacccact tcaaacaaat acatgtgtcc atttacaaag tgaataaacg
     1021 tcaataaaaa atccttcact ccttttgttg ttcttccaga ttgttcaatt tgttttatta
     1081 aagtcgctag aattgcattt acaacaatta aatcgaatag gcttttgaaa agttttcctg
     1141 ctgttaattt ttgcctggta aattgcatga ctaattgagt aaaaatagac aatagcatga
     1201 caaaaatcaa cgtcattagc actgtaaaaa tgtctgatgc tgcaatccta ttgggatcat
     1261 ttatcaagta ataagccgtt ccaaatactc caatcgagta aacggttaaa tcaagaataa
     1321 gtcgtgcccc gtaactacaa agttttccta atggccataa ttgcaatacc caaaatttct
     1381 ttcgtttgct atttcttttc agaggaagcc aggtgtgttc aatccagtcg tccgatttca
     1441 tttcgatttc ttccctaaat tctttccttt aacgttttcc tttgctactt tagcagcgat
     1501 aggttgctca atgaaccaat tttttaagta atattatttg tcaaattaag cgagacaaaa
     1561 ctttccattt acgtaactca aaaaaacttc taatggtgtt tggtaattta gtgattttct
     1621 agggatatgg tttcttttgg acgcaacgga tgagatgaat ccttgattga cttggtt
//
LOCUS       DAPCMU010000255         1664 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPCMU010000255 DAPCMU010000000
VERSION     DAPCMU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.264"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            131..505
                     /locus_tag="RD744_002834"
     CDS             131..505
                     /locus_tag="RD744_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDU0558762.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="RD744_002835"
     CDS             complement(619..1248)
                     /locus_tag="RD744_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDU0558763.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="RD744_002836"
     tRNA            complement(1382..1454)
                     /locus_tag="RD744_002836"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAPCMU010000256         1655 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPCMU010000256 DAPCMU010000000
VERSION     DAPCMU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.265"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..1097)
                     /locus_tag="RD744_002837"
     CDS             complement(<1..1097)
                     /locus_tag="RD744_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0558764.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..>1655)
                     /locus_tag="RD744_002838"
     CDS             complement(1227..>1655)
                     /locus_tag="RD744_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDU0558765.1"
                     /translation="LLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFV
                     GSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINV
                     LPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          517 a          332 c          271 g          535 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataa
//
LOCUS       DAPCMU010000257         1626 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPCMU010000257 DAPCMU010000000
VERSION     DAPCMU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.266"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            98..1576
                     /locus_tag="RD744_002839"
     CDS             98..1576
                     /locus_tag="RD744_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558766.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          584 a          226 c          296 g          520 t
ORIGIN      
        1 acaagcataa tccatcataa aatcagtata ggtgaaaaga tgaactttca tctgcatatc
       61 aagtaattgg tatgaggcat tttttgaggt gtaggatatg aagttggatt accttataga
      121 agaggaagat agagaaaaac tgctgctagc tagatattta gaatttgaga aaaagaatta
      181 tgtagtgctg cctaagttat atgaaacatt aggtttgtct aaatttaaaa caagaaatta
      241 tatcatagaa ttaaataaag aatttactga attcaaaaca aagccagaga tccaaatttc
      301 agaagaagca gagatccatg tcgaacgtct gaatttgacg gatatcaaaa atttaagaag
      361 aatctacttt caacgatcaa aaattgctcg actgttaacg gagatcgtgg attcaaaagt
      421 aacgatcgaa aaatttgcag agaatcattt tttaagcata tcaagagcct atgtaaaaag
      481 aagagaatta gtctcttttc ttgaacagca tcaaataaag ttaaagaaaa acatattagt
      541 tggggaagaa ataaacatac ggaacgtatt atttggggta tatttcgggg tctttaatgg
      601 tttttctatg ccatttagag aaagcgtcat gaatgaagca aagagtatcg tgcaatattt
      661 tgaatatctg ttcaatttaa aactgtctgc aacaaaacga atcaaattca atttgctaac
      721 cactattagt ttgttgaggg cctatgggaa tcaaccaatc ggaaaacgtt tcttttcaga
      781 tgaatacttt aagagctcag aaggaatgaa gatcatcaag gatttatcac atatttcttt
      841 tcttcatatc cctaaagagc agatcattga tgagatttct tatatcatgt tgtttttgat
      901 cacggaagaa gaatttgaat tagatggcgc atttggactt gcggaagctc attttcatga
      961 tatccacatg attagtcaaa aaatgtcagt ggaattgatt gatcaattac cattttcgat
     1021 tgaaatctct gaagaagaaa gactatatat acgaaaagaa cttcgtatgg gtatgatgca
     1081 tgtcaatcga aaaaattgga tattcgactt tgagatgact agctttacgt ctaaaaagca
     1141 gctacagttt ttcttcgaga cgtatccaat attcagtaca gcaatccaaa aggtcataca
     1201 agagaataaa cgctatttct tgggaaaaga tgaacgtttc atgattcgct tattttatga
     1261 ttatctcttt ttattggtcg gtatttgtcc ggtatcatat tttgaagcac cgattcatgt
     1321 ctgtattgat ttttcgcaag gatctgctta taccgattac attatttctc aagtgaaagg
     1381 atttaaaaat tacaatattg tgattgagag tcgaatcact tctcgtacga atatttattt
     1441 atctgattgt ttacttgaaa aatttaataa agaacagata atttggaaaa atccaccgac
     1501 tccagatgac tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac
     1561 tgaaaaaatc agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt
     1621 tatgtt
//
LOCUS       DAPCMU010000258         1626 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPCMU010000258 DAPCMU010000000
VERSION     DAPCMU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.267"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1025
                     /locus_tag="RD744_002840"
     CDS             <1..1025
                     /locus_tag="RD744_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDU0558767.1"
                     /translation="SKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDN
                     ELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGS
                     LVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVD
                     VAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSD
                     INYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIK
                     CHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEY
                     KHDILI"
     gene            1081..1299
                     /locus_tag="RD744_002841"
     CDS             1081..1299
                     /locus_tag="RD744_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558768.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1370..>1626
                     /locus_tag="RD744_002842"
     CDS             1370..>1626
                     /locus_tag="RD744_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558769.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTI"
BASE COUNT          610 a          191 c          310 g          515 t
ORIGIN      
        1 atagcaaaaa acttcagatg ggtgaaattg ttagaggtcc tagtcaaata tctattgatg
       61 ttacatataa atgtaatttt agatgtaaac attgttacaa tgcgagtggt ggagacaatt
      121 gttttatgga taatgagttg actgatgatg aactgttaga acttacaaaa gatatatgta
      181 atttaaagcc atataatatt tgtttctgtg gaggagaaac tttactgaga aaaaaaatag
      241 ttttatcttg tgccacttta ttaaaagagg caggtattcc taatattgct attgttacta
      301 atggatcatt agtaactcag gatgttgcaa aagaattatt caaagcaggt attaatcgat
      361 ttcaagtaag tttagatgga aaaaatgcta gtagccatga agcacttaga ttatatccaa
      421 acagttttga aaaggcaatt aatgctatca aattatttag agaattgtcc gaagaagtgg
      481 atgttgcttt ttgtcctact agttttaatt acaatcaatt acgagaagta tatcaactag
      541 taaatactct taaagtgaag tctttaagaa ttcaaccatt tatgcttagt ggtaggggaa
      601 tgcaaaatag ggaaatagtg ccaactgttt ctcaatatag aaaactagtt tctgatatta
      661 actatattaa aagaaatgaa gattatacca agatagattg gggagatcca attgatcata
      721 tcatccgaca cagaacttta ttgaaagatt tttcgaattt tgtttctatt aggtcagacg
      781 gaggattaat ggcatcacct tacttgccaa ttcaagttgg aaatattaaa tgtcacagtc
      841 tttctaaata ttggcgagca ggactttata aagttttttc tttagattta gttcaagata
      901 tagctactca agtttcttca atacaagata tgagtaaaga attaaaaaaa ggacatatta
      961 aatggcttaa tgaagatata gaaattgata ttctcgatga atacaaacat gatattttaa
     1021 tttaggttta tcattggaaa gtttagataa acaaagtact tttagaaagg agggaaaaaa
     1081 atgatttctg aacctcaaat gaataatatg tatgaaagcc caactataat gggagctggt
     1141 gaagctgcgc caagagcttg ttcagcaatt atggtgtgtg tgttattagc atctgcagga
     1201 gtaggtatcc aagcaggagc ggtgtatact tatgctgtag ttgctacagt agctgtagct
     1261 gcagccgcag tttggaaaac agtaggatta gttggttagg aattaatagg agactaactg
     1321 tctcctattg attccaatga ttaagctaag gaggaatttt aagaaaataa tgaaatacaa
     1381 taatttaaaa ccgaaattac ttaatgagaa catagaatat ataagagaag aaaattatgg
     1441 atatttaacc atagttccca aaaatggaac tgaattgaaa gataacgttt tcaatccaac
     1501 agtaagatat atattggcta actgtactgg aaaaaacagt gtaaaaaaaa tagcgaatgg
     1561 tttaaaaaag aagtttccta gtttagaata tgaaaaattc tatcaagatg ttgtaggaac
     1621 tattaa
//
LOCUS       DAPCMU010000259         1617 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPCMU010000259 DAPCMU010000000
VERSION     DAPCMU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.268"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            57..317
                     /locus_tag="RD744_002843"
     CDS             57..317
                     /locus_tag="RD744_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558770.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(679..918)
                     /locus_tag="RD744_002844"
     CDS             complement(679..918)
                     /locus_tag="RD744_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558771.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1204..1518
                     /locus_tag="RD744_002845"
     CDS             1204..1518
                     /locus_tag="RD744_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558772.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          559 a          218 c          285 g          555 t
ORIGIN      
        1 tattttaatg gtattatcat tttgaataat gaaaaaacat taaggatgga tgaaatatga
       61 ataacgaaac tatcatttta ggaaaaaaat atatttgcca acctattggc ttgaaacaaa
      121 aagtggttgg agaagtaatc agtaaattag aaaattgtgt tgtgatctgc gtagacgaat
      181 atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg aaaagtagtg gcaaaatata
      241 ctcacgttta tgagttggaa acaacagaat atctatataa ctatagcaac aaatatgaac
      301 atactttagt ttcataaaga tttaaataaa gtgccttcaa aagcgagaag accaagcttt
      361 tgaaggcact ttatttagtt ggcagtgatt gttcatgatt tttgatttat tcaaaaggtt
      421 cgcctagaga atctgtctct gtaacgaggc ctggtggaga aactcgataa aaacctatac
      481 tgccagaact gtcctatttt cggatagaca aactagtagt tttaaaaaga tcatccgatc
      541 caatcatttc aaaaaatgca aaactgatat caggatcatt tagctcaggc ttgttatcgt
      601 aaagcatttg taaaggttgc atttagttag gagtaaacgc gaaactatca tcattatttg
      661 ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa taaaggagac gtgctgacag
      721 tttctttacc ctgttttttg cagaggtaga aacttttata tcggaagttt ctaccggaga
      781 atttttctca tttgtagaaa ttgttgaatc gatactattc gaaatttgcg tatctgaagg
      841 tatgttcttt gatgcagaag tcccacaagc agtaagtaac aataaatttg ataaactgat
      901 aaacagaatt cttttcatta tgtacacact ccttatagta tcgtttagca tacacctatg
      961 ctcagtatta ggcaaaatca atgcaaaact atcaagtctt ttatagtatc tattagtctt
     1021 agtaatacat atatggagag ggtgatatcc cagttcaaac cagatacatt ggttaccatc
     1081 tgtattaacg attatgcctc taaacgcttt ttttatgaaa aaatttataa gtgaaaacgt
     1141 aggtatgata ttatctagag gtaatttttt tgggtattta aatagttgct aggaggatag
     1201 cttttggatt tttacagtaa ttttatctta ataatagcaa tattattact tctaaatatt
     1261 tggttttttg ataagagtag aaatgcaggg ataggtttta gaaccaaacg aagtacgtct
     1321 tctgaaaaaa agtgggtata ttcacagact attttttacg gaggggttat ttcaatcagt
     1381 ttactttctt caacacttta ttcttttaat gttatagacg tttccatgtc aaattttata
     1441 tctattattg gaatccttat ttctgcaatt attacacaac tacttcttgt gtttgaagag
     1501 aagtcaaaaa ataattaaag caattatgcc ttttagtaca ggaattatta cttcttacaa
     1561 atgtatagcg aatgtgaagc ctgctgtttt aattgttcta gaaaaacttt tgagtga
//
LOCUS       DAPCMU010000260         1611 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPCMU010000260 DAPCMU010000000
VERSION     DAPCMU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.269"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            63..1610
                     /locus_tag="RD744_002846"
     CDS             63..1610
                     /locus_tag="RD744_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDU0558773.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          544 a          290 c          333 g          444 t
ORIGIN      
        1 tcttgtgcgt ttcttgtggt attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa
       61 ttttggaaac tacagatacg ttgcttcagt tgcttcaaga agcccataaa accaatcaag
      121 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
      181 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      241 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      301 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      361 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      421 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      481 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      541 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      601 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      661 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      721 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      781 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      841 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      901 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      961 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
     1021 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
     1081 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1141 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1201 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1261 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1321 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1381 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1441 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1501 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1561 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa a
//
LOCUS       DAPCMU010000261         1585 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPCMU010000261 DAPCMU010000000
VERSION     DAPCMU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.270"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..627
                     /locus_tag="RD744_002847"
     CDS             <1..627
                     /locus_tag="RD744_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDU0558774.1"
                     /translation="GDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTS
                     ITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLIS
                     SVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIML
                     KSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            644..1078
                     /locus_tag="RD744_002848"
     CDS             644..1078
                     /locus_tag="RD744_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558775.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          585 a          183 c          276 g          541 t
ORIGIN      
        1 ggagatattc gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt
       61 gttagtggag atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta
      121 gcacaaacgt ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct
      181 ttgatacaag aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct
      241 gggattgttt attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta
      301 atttcttctg tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa
      361 atagctaatt atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg
      421 catattacga tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg
      481 aaatcaaacc atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac
      541 ttttcgagag tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta
      601 gtggacaatt tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt
      661 acgtagtttg gaggagttga ggaaattgga ttttaacaaa aaattttttt gggaaatttc
      721 tgatttgagt tgtttcgtag aatctgagcg tctaagtaat ggtaataata gtgtgttaaa
      781 tgaatatgac aaagtagtaa ttaataaagt aaaaagttta tgtgaagaag ctagagaatc
      841 tatcagtcat attaatggta gcaattttaa acaaacatta tttaatctga ttaagctaga
      901 tgctaaaata tcaagttatt tattcttttt gatacatgat gaatttatgg attatcaaaa
      961 attggatcag ttaattgaaa gagaaagctg ggaagattat tacgtgggtc taactttttc
     1021 agaaaagtta aacaactaca agttaataga ttataagtat ttgagtgaaa gcaattaatt
     1081 attcatgtag agtactagac actttaaata tggacaattc aattataaag aaaaagaatc
     1141 aataatttaa tgaggctgtg acaaatgtca ttttttgttt cgctaaaaac gagtgaaaaa
     1201 atgttgataa ctccaatcat tcgtttaaga ttctaggaat cattcagaaa tgctgtgaaa
     1261 ccgatacttt ataattataa agaaggtgat ttcaaattcc gaagaaggaa tttgaagtca
     1321 cctttttact tatgtcacag tctcattcag taatgcggga taaggctcct tattccgtta
     1381 tttgaatact ctgaagttta atctaagaaa gggtaatatt ttgaaaacat gcttcatcgc
     1441 atagaaatga acttaatttc agaaagaata catacatatc tttttatatg ttttcagctg
     1501 ctgaatggtt aaaaagcaca aatatccgaa aaaaacatga agaaatgtac tttttttgtt
     1561 gtaatgttca tgaaattctc taagt
//
LOCUS       DAPCMU010000262         1561 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPCMU010000262 DAPCMU010000000
VERSION     DAPCMU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.271"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(99..1229)
                     /locus_tag="RD744_002849"
     CDS             complement(99..1229)
                     /locus_tag="RD744_002849"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDU0558776.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          448 a          311 c          249 g          553 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 g
//
LOCUS       DAPCMU010000263         1546 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPCMU010000263 DAPCMU010000000
VERSION     DAPCMU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.272"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(202..1017)
                     /gene="thiD"
                     /locus_tag="RD744_002850"
     CDS             complement(202..1017)
                     /gene="thiD"
                     /locus_tag="RD744_002850"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDU0558777.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1311..>1546
                     /locus_tag="RD744_002851"
     CDS             1311..>1546
                     /locus_tag="RD744_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558778.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKE"
BASE COUNT          471 a          309 c          246 g          520 t
ORIGIN      
        1 aacaaacatg agaagctgtt ttttgaactt gttccttgtg actcggagct ggacggcagg
       61 ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
      121 aagttaaaaa cataagaagc tgtttttact tggctgttct tattacttga tgcagggcaa
      181 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
      241 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      301 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      361 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      421 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      481 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      541 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      601 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      661 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      721 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      781 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      841 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      901 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      961 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
     1021 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
     1081 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
     1141 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1201 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1261 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1321 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1381 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1441 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1501 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatt
//
LOCUS       DAPCMU010000264         1529 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPCMU010000264 DAPCMU010000000
VERSION     DAPCMU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.273"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..1408
                     /locus_tag="RD744_002852"
     CDS             <1..1408
                     /locus_tag="RD744_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDU0558779.1"
                     /translation="TVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNT
                     PGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVT
                     NLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYP
                     DGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTW
                     KEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGK
                     TEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVV
                     VSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLI
                     LLGTLGLLGYKNRKKENE"
BASE COUNT          606 a          232 c          334 g          357 t
ORIGIN      
        1 aacagtagaa ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac
       61 taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat
      121 tgataccaac acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac
      181 gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata
      241 tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa
      301 tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg
      361 tacaattgat accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga
      421 cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga
      481 taaatacaat cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc
      541 tgagggaatt aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa
      601 agtagatgtt agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg
      661 atcatctgat gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa
      721 atataatcct gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga
      781 gggaattaag aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt
      841 agatgttagt acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc
      901 taaagaagaa gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca
      961 agttgatcct attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga
     1021 ggtgacagtt acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa
     1081 ttttacagtt aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga
     1141 tgaatctggt aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag
     1201 tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga
     1261 caaaaataga ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag
     1321 taatatcttt actattagcg gtggtttaat actgttagga acgttaggat tattagggta
     1381 taaaaatcga aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga
     1441 ggaagaatct ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa
     1501 gatgaatata ctttttatag aaaggaaaa
//
LOCUS       DAPCMU010000265         1520 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPCMU010000265 DAPCMU010000000
VERSION     DAPCMU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.274"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            6..1175
                     /locus_tag="RD744_002853"
     CDS             6..1175
                     /locus_tag="RD744_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDU0558780.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          545 a          270 c          244 g          461 t
ORIGIN      
        1 ctattatgaa aaaaggaaaa catgctaaaa aaagaaaata ttcattttta atgatcgttg
       61 tcgtgttgtt tagccttagt gcgttctcta caggaactat tcttggatgg caattttaca
      121 ataatgaaca ctctcagata gttgcccatg ctcaatcagc tcaaaaaaag ttgatactga
      181 ctaccaatga acaacagctc gttgaaaaaa tcgatcaact attaagaaaa aatgattata
      241 ttggctcaat ttacgtaaag aaaaataatc gcatcatcat ggaaaaaggc tatggctatg
      301 cgaataaact cacgcaacaa ccaaatgatc cttctcttta ttatcagatt ggttcggttc
      361 aaaaagcatt gactactctt ttgattttaa aacaagtcga gcgtgggcac ctttctttag
      421 atactaagct ggctcaattc tatccacaaa ttgctggcag ccagcaaatt actatccgtg
      481 acttgctgta tatgcgctca ggcttgcacc gaactgcttc tccaaccata ccgatgactg
      541 atgaagaagt cgttcaattt gcgattcagc acttaaaatt tacagattat cagacctctc
      601 attatgattc attgaacttt accatattga ctggaatttt gatcaaatta acaaatcaac
      661 cgtatgaaaa attattgacc gatgatatca tcgaaccttt aggattacag catacaaaat
      721 tttatgacca agtcaaaaat tcatccaatc atgcactatc ctatcaaatg tctaaagagg
      781 atgattactt taaaccactg acagaatctg aaacagagat ccataacgaa ttagggactg
      841 gtaatatcag tatgtccgtc tatgatttaa atacgctgtt tacacaagtt ttatctggaa
      901 aactagtttc caaagactta ctcttctcat tatggcgtga aaatgcgaat ggacatccat
      961 atagtggtgg ggtatatagc ggagataatt atatcctgtc gcaaggaaat atcgactctt
     1021 ttgatacatc tgctatattt agcaaagatt cacaggatgc tatcatcatg gaaagcaata
     1081 taaaagctga caaaactatc aaactgccta ctgctgatct aagaaaccaa atctatgacc
     1141 tgatggaagg aactactaag ttggcaaata gctgatatga tgaaaagata gcaaatacta
     1201 cttctttgaa tagcatttgc ttaaaaaaag tactcgacta aacgtaggaa agagtgtggg
     1261 acaaatctaa aaatcaattt ttgctacaga ctttaattcc aaataaacag aacagaaaga
     1321 ggtgttcttc tttctgttct gtccaaatta ttctaagatt tctctttacc aacattattt
     1381 gacaaagggc ctattaaatc taaaatagta tctttagatt gctaaaatat gtgctagcaa
     1441 atcattcatg gcttgttctt atttttaatt ttatgcaata aaaaagaaga acaattagcg
     1501 tgattataga aataaataat
//
LOCUS       DAPCMU010000266         1517 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPCMU010000266 DAPCMU010000000
VERSION     DAPCMU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.275"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(124..558)
                     /locus_tag="RD744_002854"
     CDS             complement(124..558)
                     /locus_tag="RD744_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDU0558781.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(658..1506)
                     /locus_tag="RD744_002855"
     CDS             complement(658..1506)
                     /locus_tag="RD744_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDU0558782.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT          536 a          301 c          239 g          441 t
ORIGIN      
        1 gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc
       61 tttctcgaat agcaaacata tacaaaaaaa tatcagagac aaaaactaat ctcatcataa
      121 acattacata tatttctcaa aaagttgttt tctttcttca aaacttactt cacgatggac
      181 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
      241 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
      301 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
      361 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg tgccatacaa
      421 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
      481 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatctat
      541 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
      601 tttattatac cagttaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
      661 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
      721 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
      781 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
      841 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
      901 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
      961 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaacaaacag
     1021 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     1081 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     1141 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     1201 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaaaca agcttccgaa
     1261 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     1321 attcaacact aaaataacta tagctcggtc aaatcctaaa acagcttcca gtaatattcc
     1381 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     1441 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     1501 tttcatttat ccacttc
//
LOCUS       DAPCMU010000267         1513 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPCMU010000267 DAPCMU010000000
VERSION     DAPCMU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.276"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            3..1469
                     /locus_tag="RD744_002856"
     CDS             3..1469
                     /locus_tag="RD744_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDU0558783.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          414 a          289 c          319 g          491 t
ORIGIN      
        1 ttatggaaaa acagcaagac aagggcttct tcgggcagcc gaagggattg tcaactttgt
       61 tcttcactga gatgtgggaa cgatttagtt attacggtat gcgtgcatta cttatctatt
      121 atatgtacga tacagttgcc aacgggggac ttggattacc acgagcaaca gcattagcta
      181 tcatgtcgat ctacggatct ttgatctaca tgtctagtat cgtcggcgga tgggtatctg
      241 accgagtgtt aggagcaaaa cgcactgtat tcttcggtgg agttttcatt atgcttggcc
      301 acatcgtctt ggctttacca tttggtgtaa gtacattgtt tgtttcgatg gcattgatta
      361 ttatcggaac cgcctttttg aaaccaaatg tttctggcat ggtcggacat ctttattcaa
      421 agactgactt gcgaagagat gcaggatttt cgatttttta catgggtgtc aacttagggg
      481 cattattagc accactaatc gttggtacaa tcggtcaaga atataactat catttagggt
      541 tctctttagc tgctattggg atgttctttg gattattaca atacatcatc caaggacgca
      601 agacattaga cggagtgggt acaacaccgc cgaatccttt gacaactgaa gaacgtaaag
      661 gattcatccg taacttgatc atcgcacttg ttgttatcgc tgcagtattc ggcggggcac
      721 aactaactgg tcatctgaca attgactttt ttgtcaatac aatcagtgta ttggggatta
      781 tcttaccgtt gtattacttt atcaaaatgc tgacttcaaa agatgtaact gctgaagaaa
      841 aaccaagagt atgggcttat atcccattat tccttgcagc aatcattttc tggtcattac
      901 aagaacaagg ttcatctatc ttagctttat ttgctagtga acgtacacaa tctacgctat
      961 ttggtgtaac gatccaagca agctggtatc aatcattgaa tccaattttc atctttattc
     1021 tgacaccagt attcgtaacg atgtggacga aacttggtga acgtcaacca tctacagtag
     1081 tgaaattctc tcttggactt ttatttgcag gattatcgta cttgctattg atgttgccgg
     1141 gtatgcttta tggtacaagt ggaaaagtaa gtccattgtg gttgttggga agtttcttca
     1201 tcgtggttat tgctgaattg tgtctttcac cagttggttt gtctgtgaca acaaaattag
     1261 caccacgagc atttgaatca caaacaatcg ctatttggtt cttagctgat gcttcttcac
     1321 aagcaatcaa tgcacaaatt gcacgcttct acacaccggg aacagaatct gcttacttcg
     1381 gtattatcgg tgcagtagca atcgtcggtg gtatcttgct atttactgtg aaagaaccaa
     1441 tcaaaaaact aatgggctct attcattaat caatgaacat aaaaacagga attgcttatt
     1501 tgcagcagtt cct
//
LOCUS       DAPCMU010000268         1509 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPCMU010000268 DAPCMU010000000
VERSION     DAPCMU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.277"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            101..868
                     /locus_tag="RD744_002857"
     CDS             101..868
                     /locus_tag="RD744_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDU0558784.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="RD744_002858"
     CDS             1060..1458
                     /locus_tag="RD744_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDU0558785.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DAPCMU010000269         1505 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPCMU010000269 DAPCMU010000000
VERSION     DAPCMU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.278"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(348..431)
                     /locus_tag="RD744_002859"
     tRNA            complement(348..431)
                     /locus_tag="RD744_002859"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:cag)
     gene            959..1459
                     /locus_tag="RD744_002860"
     CDS             959..1459
                     /locus_tag="RD744_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558786.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          480 a          257 c          245 g          523 t
ORIGIN      
        1 tgggaagcaa acggttggta ctaagattca ttatttgaac gtttaaacac attcaaaata
       61 tagtaagagg tcatgcgatt tattctttcg catgacctct ttttgagttt aagtttagtc
      121 tttattcctt aactatcgtt tgccagtatt tcttgtatca gaatctataa atctactatt
      181 tgaatcaatc tattaggttt taaactgtcc caccttcaca caaatttttt ttgatttatt
      241 tgaatctggt tccgttgttt aaaatttttc ctatattctt ttagataata tgttattttc
      301 tttctcatcg ctccaggtgt accttctctt tataaaaaaa caaattatgc ggctaagaag
      361 actcgaactt ccacgaggtc accctcacta gcgcctgaag ctagcgcgtc tgccaattcc
      421 gccatagccg catcacatct tttagtttag gattacatgt gttttttgtc aagaacatat
      481 ttttgttctt tttcatttct gtacgccctc ccttatgcca tctacacaaa taattcatcc
      541 gagcaactcc atcatctatt gagggttggt gaaaaaatct gttttatcca tatgatcgat
      601 ttcaatgtta aacgaacgac tctgggatta gtaactattt tatttttttc ttcatttctt
      661 ttttcctctt tttctcgatt cagctatctt ctttcatttg gatattgatt atttcttctg
      721 ttgattacta tccttctgga attctagacg ctgaagtaat tcacgttttc tttcttctag
      781 agatcataag taacgaatga acgaacttca aataattttt ttattgtaat tcatatttgt
      841 attgttcgat gattcttaat acgatatgac acaataaata aaaataggca tggctattta
      901 caacaatagt gctatagtag aaaacaaatg aataaggtta cttaaagagg tgaatacgat
      961 gaaactgatc aaactatggg aagcagattt aaagaaagct tatcagctac aaactagctt
     1021 tggaccaaat gagagtggat ttatcaacaa tgcttatggc tatacatttg ctgaattttt
     1081 agattatgta aaaaaatgcg aaaaaacgtc tttaggaatc gatcttcctg agagttacgt
     1141 tcccgcaacc gtctttattt tagaaagtga tgaacatgag tatgtaggaa tttttaattt
     1201 gcgccataaa ctgaacgaaa cattaagaaa cggcgctggc catattggtt acggcatctc
     1261 ccctaattat cgtaaaaaag gatatgccac acaaggatta gcattagtac tacaagaggc
     1321 aaaaaaaatc ggcattgatg aggcactttt gtctgtaaat aaaaaaaatc ttggcagtct
     1381 taaagcacaa caaaaaaatg gggctgcgat ttatcgtgaa gatgagaaag aatactatac
     1441 gaaaatcaaa ctgatgtaaa aaagaaggat tccaaaagga aagggatttg aatgtccttt
     1501 atcaa
//
LOCUS       DAPCMU010000270         1495 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPCMU010000270 DAPCMU010000000
VERSION     DAPCMU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.279"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..221
                     /locus_tag="RD744_002861"
     CDS             <1..221
                     /locus_tag="RD744_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDU0558787.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="RD744_002862"
     CDS             218..1237
                     /locus_tag="RD744_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDU0558788.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT          487 a          282 c          313 g          413 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta caggg
//
LOCUS       DAPCMU010000271         1452 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPCMU010000271 DAPCMU010000000
VERSION     DAPCMU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.280"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..625)
                     /locus_tag="RD744_002863"
     CDS             complement(<1..625)
                     /locus_tag="RD744_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDU0558789.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="RD744_002864"
     CDS             complement(784..1050)
                     /locus_tag="RD744_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558790.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="RD744_002865"
     CDS             complement(1040..1396)
                     /locus_tag="RD744_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDU0558791.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aa
//
LOCUS       DAPCMU010000272         1449 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPCMU010000272 DAPCMU010000000
VERSION     DAPCMU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.281"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            25..537
                     /locus_tag="RD744_002866"
     CDS             25..537
                     /locus_tag="RD744_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558792.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            808..1431
                     /locus_tag="RD744_002867"
     CDS             808..1431
                     /locus_tag="RD744_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558793.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          568 a          178 c          249 g          454 t
ORIGIN      
        1 aaagttctaa gtttgtgatg ataaatgaaa ttaaatagta taaatgattt atttgctatt
       61 ataggtgtta ttgctaacat acttttttcg tgtttgaatt ttagatttac tcaaaagaac
      121 aaaaagaaca aactcagtat agaacaaatg aaaaaagaca ttgaaactga tttaaaaaat
      181 gaccataaag aaagaagaaa aattgaagaa tatacttctt taatggaaaa gtgtaaagca
      241 actgatgcaa tttttagctt tttaagaaaa agtgatgaga taatcacacg tgaaaatata
      301 gatttagaaa atgagattag taaacttgaa tctatctttt ccgatataaa ttctcttaag
      361 tatttaatcg tcgaagaaaa ttcggatagc tttttagaat ttgaaaaata tctaacagat
      421 cttatagata atagaaataa tgaattaatg agacaaaaca ttgcaagatt ccttactaaa
      481 ttagtttctg aatggaaaag tattatttct caaaatatgg ataaattaag atattaagga
      541 tgttagtttt aaaataaact aatgtcctta tttttgtgtt cacatttctg ttacctaaga
      601 aatacttaaa ggaggtgatg tagccagcaa cgactacaac ttgaacccgc tatctacagt
      661 ttatttgttg tttcttttgg gtatactcta atggtacaaa tccatatttg cttaaagcgt
      721 tgatttttca gcggtttttc tttattgcaa agtgtaccct aagacctgta tactaatagt
      781 aaatagattg gataggaatg atggcaaatg tatttagctt atttaagagt ttcttccgac
      841 gatcaaagtt tagctcggca acataagcta attgaaaatt ggtcagtcaa aaataatatt
      901 gctgaagacg atttaaaaat tttcgaggaa aaagtctctg gaaaaaatat tgaaaacaga
      961 ccagagctgc aagagcttat ttctttcata cgtgaacgag atacgattat tattcctagt
     1021 cttgatcgat tagcaagaaa tagtaaagat acaaaagact tacttcaatt gtttcgtagt
     1081 aaaggtgctt ctattgaaat attagaccta ccgtcgttta atggggtaac tgaccctgca
     1141 ttaagagact tgctaacaaa tttagtcata gaagtgttta gctatgttgc tgaaaacgaa
     1201 cggaaaaaaa tcaaagagcg tcaaaaacaa ggcattgaaa ttgcaaagaa aaatggtcgt
     1261 tatactggta aaaagattca ataccatgcc aatgcaacag gaaaaaacaa attgatttac
     1321 gatgaaattg ttgatggtct gaagaaaaaa gaaactattt ctaaaatagc aaagaacgca
     1381 ggagttacaa gaaaaaccgt ttatcgagta aaaagagatt taggaatgta atatgttcca
     1441 gttagaaag
//
LOCUS       DAPCMU010000273         1437 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPCMU010000273 DAPCMU010000000
VERSION     DAPCMU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.282"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(943..1356)
                     /locus_tag="RD744_002868"
     CDS             complement(943..1356)
                     /locus_tag="RD744_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDU0558794.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          530 a          237 c          211 g          459 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataa
//
LOCUS       DAPCMU010000274         1428 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPCMU010000274 DAPCMU010000000
VERSION     DAPCMU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.283"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(243..464)
                     /locus_tag="RD744_002869"
     CDS             complement(243..464)
                     /locus_tag="RD744_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558795.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="RD744_002870"
     CDS             complement(562..807)
                     /locus_tag="RD744_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558796.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            991..1428
                     /locus_tag="RD744_002871"
                     /pseudo
     CDS             991..1428
                     /locus_tag="RD744_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          477 a          276 c          210 g          465 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taactttgaa tcggctacac tagactagac agaaaaaata aggtgtgtag
      961 aataataaaa aacacactgg aggatttttc atgccaagac gtcaacgaag aacctattca
     1021 aaagaattca aacaacaaat cgtcgatctc tatctcgctg gtaagcctcg cgcagaaatt
     1081 attcgagagt atgagcttac gccttcttct ttcgataaat ggatgaagca agcacaatca
     1141 acgggctcat tcaaagaaag agacaactta acaccagaac aagcagaatt gatcgcacta
     1201 agaaagaaaa ataagcaact cgaaatggag aatgatattt taaagcaagc ggcgctgata
     1261 ttcggacgaa aagacaagta attgatgcca acaagcataa atattccata tcagcgatgt
     1321 gcaaaattct aaatatttct cgtcaaacct actattatca agcgaaaccg atcgaaaatg
     1381 agtccgactt agaagagatc gttcaggaag agtttattcg aaaccgaa
//
LOCUS       DAPCMU010000275         1427 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPCMU010000275 DAPCMU010000000
VERSION     DAPCMU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.284"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            105..1406
                     /locus_tag="RD744_002872"
     CDS             105..1406
                     /locus_tag="RD744_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0558797.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          471 a          287 c          262 g          407 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctacct
//
LOCUS       DAPCMU010000276         1415 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPCMU010000276 DAPCMU010000000
VERSION     DAPCMU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.285"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            146..229
                     /locus_tag="RD744_002873"
     CDS             146..229
                     /locus_tag="RD744_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDU0558798.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            354..1091
                     /gene="erm(B)"
                     /locus_tag="RD744_002874"
     CDS             354..1091
                     /gene="erm(B)"
                     /locus_tag="RD744_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDU0558799.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          515 a          220 c          237 g          443 t
ORIGIN      
        1 tgcgtatggt taaccctaaa gttatggaaa taagacttag aagcaaactt aagagtgtgt
       61 tgatagtgca ttaaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
      121 tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atgcgttatg tagataaaac
      181 atctactgtt ttgaaacaga ctaaaaacag tgattacgca gataaataaa tacgttagat
      241 taattcctac cagtgactaa tcttatgact ttttaaacag ataactaaaa ttacaaacaa
      301 atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagtgat tacatgaaca
      361 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
      421 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
      481 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
      541 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
      601 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
      661 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
      721 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
      781 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
      841 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
      901 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
      961 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     1021 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     1081 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     1141 aatgtagata aattattagg tatagggcac ctctaataac taccaattaa ttcacctcaa
     1201 aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt tcttatcgat acaaattcct
     1261 cgtaggcgct cgggacccca acatgaggaa atgtttttat aaaaaaagag tagttaccgt
     1321 tttttggtaa ctactctttt ccttttttaa ggtttaaata cctggaagtt taggtgtttt
     1381 gggtattggt ggtgtaggtg gctgcaagct ttcca
//
LOCUS       DAPCMU010000277         1393 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPCMU010000277 DAPCMU010000000
VERSION     DAPCMU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.286"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(139..438)
                     /locus_tag="RD744_002875"
     CDS             complement(139..438)
                     /locus_tag="RD744_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDU0558800.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            789..>1393
                     /locus_tag="RD744_002876"
     CDS             789..>1393
                     /locus_tag="RD744_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDU0558801.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFA"
BASE COUNT          468 a          225 c          199 g          501 t
ORIGIN      
        1 aggataaaat acaatataaa gaaacatctg tttctttatt tagaataaaa aaatacagac
       61 tctgttcaaa taaatgaacc tagcctgcat tatgagattg actatacttt ttaattcatt
      121 gagatgaata actcgttttt ataaatgccc aaaagtaatc ggtgccgaag ccattcctcg
      181 acctaattct ttttcaaatt ttcctattgt tgatatctcc atataaaacg gtgatactgg
      241 tttagacaat tctcccctga ttgctaacgg acgtaaacta cgttccaaat tatttatgac
      301 tttaggataa tactgacctt tttctaagcc tgtctttgcg tctatcaaaa gtatacgttc
      361 cttttctttg atctctggat ttaaaacgag atcatataat tttagtacca tgttttctat
      421 ttcttcactt ttagacatca cgatttcctc ccaaaaatat atttcttccc actacattct
      481 ataaacaaag tatacgctac atctagattt ttttaaatca ttactggatt gttaaaacct
      541 ttcaatacac tgttatggca acgattttta ttttaaaaac attctaaatt aaatcagtaa
      601 aaatttttat tctaagattt ttattagaat tacgcctaat ttgtgtctct cagggcaatt
      661 ctaagaacgt ttcaagagat agcgacacta tcgttatcga aaaaggcgaa aacctatctc
      721 attttctgct aatgtaagtt ggtttagctt ttgttaatat gactttatga aaatggaggt
      781 aattaaatat gatttttcct aaagttaatg aagcaagctt gaataaagga tccttttttc
      841 atatctatga aaacgaagat tttgaactgt ataatatcct ttcacaaaaa gaggtcatcg
      901 aaaaccaaat aatctgccat ccaattattt acaaatctat tttcaagtat gcattttttg
      961 cttccaatga ttttcaaaga atggagatta ccgactgtat attcgaaaac tgtgattttt
     1021 caggctgtat attgacaaaa agcatgttgc atcgcgtgaa atttgtcaat tgtaaattaa
     1081 caggtactaa attgatggaa agttatattg ggaacacgtt attcgagaat tgtaaaatgg
     1141 actatgtaaa tttatctggt tcaaacataa aagaaagtac ctttgagcac tctgtcttat
     1201 caagcgcaga ttttgtagac tgttccctca ctaaaataat gttttctact aatgatttag
     1261 aaaatgctag cttttttgat actgatttaa aaaatatcga tttaagtagc aactcctttg
     1321 aaatgattga agtacgctcg gaacatctaa aaaattgtac gcttaatcag ttgcaagcac
     1381 ttggttttgc tag
//
LOCUS       DAPCMU010000278         1375 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPCMU010000278 DAPCMU010000000
VERSION     DAPCMU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.287"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            191..445
                     /locus_tag="RD744_002877"
     CDS             191..445
                     /locus_tag="RD744_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDU0558802.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="RD744_002878"
     CDS             446..763
                     /locus_tag="RD744_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDU0558803.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DAPCMU010000279         1373 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPCMU010000279 DAPCMU010000000
VERSION     DAPCMU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.288"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            171..596
                     /gene="lacA"
                     /locus_tag="RD744_002879"
     CDS             171..596
                     /gene="lacA"
                     /locus_tag="RD744_002879"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDU0558804.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            613..1128
                     /gene="lacB"
                     /locus_tag="RD744_002880"
     CDS             613..1128
                     /gene="lacB"
                     /locus_tag="RD744_002880"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDU0558805.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1139..>1373
                     /locus_tag="RD744_002881"
     CDS             1139..>1373
                     /locus_tag="RD744_002881"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDU0558806.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSP"
BASE COUNT          478 a          195 c          305 g          395 t
ORIGIN      
        1 taaaaaaaca aataaaaaaa acaaaaaata aacaaaaaaa gtgttgcttt ttgtttgaat
       61 gcttgatatt atataaacaa agaaatgatg aaaacgtttt ttcgaacatt ttgcaaaatg
      121 ttttctactt ctacgtagca tttctttcta aaatttagga ggtggtcaaa atggctattg
      181 ttgttggtgc agatctcaaa ggtacgagat taaaagatgt tgtgaaaaat tttcttgtag
      241 aagaaggttt tgaggtaatt gatgtgacta aggacggaca agattttgtt gatgttacct
      301 tggcagttgc ctctgaagta aataaagatg agcaaaactt aggtattgta attgatgctt
      361 atggagctgg tccattcatg gtagcaacta aaattaaagg tatggttgca gcagaagttt
      421 cagatgaacg ctcagcttat atgacacgtg gacataacaa ttcacgaatg attacggttg
      481 gcgctgaaat cgtcggtgat gagcttgcta aaaacatcgc taaaggtttc gtcaatggta
      541 aatatgatgg cggacgtcat caaatccgag tagatatgct taacaaaatg tgttaagaaa
      601 aggagttaag gaatgagaat cgcaattgga tgtgaccaca ttgtcacaga tgaaaaaatg
      661 gcagtatcag aattcttgaa atcaaaagga catgaggtcc ttgatttcgg gacatatgac
      721 cacgcacgta ctcactaccc tatctatggt aaaaaagtcg gtgaagcagt agtaagtggt
      781 caagcagact tgggagtatg tatctgtggt acaggtgtcg gcattaacaa tgcagtcaat
      841 aaagttccag gtgttcgctc agcgttggtt cgtgatatga catcagctat atatgcaaaa
      901 gaagagttaa atgcgaatgt tatcggtttc ggtggaaaga ttactggtgg tcttcttatg
      961 aatgacatca ttgaagcttt cattgaagct gagtacaaac caacagaaga aaataaaaaa
     1021 ttgattgcaa aaatcgaaca cgttgaaaca cataatgcac atcaagcaga tgaagatttc
     1081 tttacagaat tccttgaaag atgggaccgt ggtgactacc acgattaagg tataacaaat
     1141 gattctgaca gtcacactaa atccttcagt agacatctct tatcctctag aaacattgaa
     1201 aattgataca gtgaataggg tgaaagatgt tagtaagaca gctggtggaa aaggcttaaa
     1261 tgttactaga gtcttgtatg aatctggtga taaagttact gctacaggtt tcttaggtgg
     1321 taaaattggt gaatttattg aaagcgaatt agaacaatct cctgttagtc cag
//
LOCUS       DAPCMU010000280         1330 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPCMU010000280 DAPCMU010000000
VERSION     DAPCMU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.290"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            25..1014
                     /locus_tag="RD744_002882"
     CDS             25..1014
                     /locus_tag="RD744_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDU0558807.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            996..>1330
                     /locus_tag="RD744_002883"
     CDS             996..>1330
                     /locus_tag="RD744_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDU0558808.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPL"
BASE COUNT          471 a          179 c          252 g          428 t
ORIGIN      
        1 tatgacttta taatacttat tagagtgagg gataaagaaa tgagacaact acacaaagaa
       61 cagattgaaa cagcaatggc agcgttaaag gaaaagacaa ttgatcatgt atattttaca
      121 gcatgtgggg gttcacaagc cgttttaatg gctggtcaat atctatttga taaagaatta
      181 actgtacctt gtcatgttta tactgctaac gaatttaact atgctatacc aaaaggatta
      241 tctgaaaatt ctttagttat cacttgttcc cattctggca caacaccaga aactgtagaa
      301 gcaacaaaaa aagcaagaga atttggagca atgactattt gtttatctaa tgaagaagga
      361 tctcctctat gggaagcagc tgaatatcct gttcactatg attggggtaa agatgttgat
      421 gctagtgatt taaataaagg aattctttat ggtttattat ttaatatttt acttgtttta
      481 gatggctcta aaaaatggga aacttgcttg aatgaatttg ataagttaaa tgatcttgtt
      541 attgctgaaa agagcaaatt tgaaataact gctaaagaat ggggtaaaaa ccaaaaacga
      601 gataaaatta tttacacaat tggtagcggt atcaattacg gagaaattta ctccacagca
      661 gcttgctggt tcatggaaat gcaatggatt aattctaatg ctattcattc tggggaattt
      721 ttccatggtc cttttgaaat cactgattat gatgtaccat tcatcttagt gaaaagtatt
      781 ggagatacaa gatttttaga tcaacgggta gaagacttca ctaaaaaatt cagtaataaa
      841 ttaatggttt tagaccaagc tgattttgac ttagaatcta tagatagtgt agcgaaagaa
      901 tatattgcag ctgtacttag tggcgttgtt attcgccaaa tggtagaagc gattgctttt
      961 gaaagaggcc attccttaga tgttagacgc tatatgtggc aaatgaaata ttaattaata
     1021 ggaggttgca aaagatggca aatcaattat ttcacattgt gattacaaca cattctaccc
     1081 tttgtgaggg ttatttaaag gcaacaaaat taattttaat gaatgacgcc acaggtatta
     1141 gtgctgttcc atttaaagaa aatactagtc cagatgattt tgaggagagt atgaaagaaa
     1201 taattagtca gcatcatttg caacctctaa ttattttaac ggatttaatt ggaggaacac
     1261 ctaataatgt ttctattaaa cagttgaaca acaatagaat tcaaattatt gctgggatta
     1321 atttacctct
//
LOCUS       DAPCMU010000281         1314 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPCMU010000281 DAPCMU010000000
VERSION     DAPCMU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.291"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            82..>1314
                     /locus_tag="RD744_002884"
     CDS             82..>1314
                     /locus_tag="RD744_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDU0558809.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT          444 a          257 c          251 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg attt
//
LOCUS       DAPCMU010000282         1310 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPCMU010000282 DAPCMU010000000
VERSION     DAPCMU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.292"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(150..>1310)
                     /locus_tag="RD744_002885"
     CDS             complement(150..>1310)
                     /locus_tag="RD744_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDU0558810.1"
                     /translation="SEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQYIQANAYER
                     SEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKALIAAMIEMV
                     ISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYPFFMCDA
                     LYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          380 a          290 c          232 g          408 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct
//
LOCUS       DAPCMU010000283         1308 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPCMU010000283 DAPCMU010000000
VERSION     DAPCMU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.293"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            26..>1308
                     /locus_tag="RD744_002886"
     CDS             26..>1308
                     /locus_tag="RD744_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0558811.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          409 a          285 c          261 g          353 t
ORIGIN      
        1 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
       61 tattctagac ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga
      121 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg
      181 ccactatcaa aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt
      241 gaatgatgtc agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa
      301 acactgtgga aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc
      361 ccgcagagtc aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat
      421 tgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
      481 taaaaccgtt tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc
      541 ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
      601 gattgatatt gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc
      661 tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac
      721 attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
      781 acacatcggt cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg
      841 ggatcgtcgg tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga
      901 attgaatggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga
      961 gatagtcgat cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca
     1021 ggtttttctt tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
     1081 agactacaag gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg
     1141 aacagaaatc aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa
     1201 caaccatatc aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg
     1261 agaacgaatc tttttatatc gaggaaaata tttcaagaaa actaagaa
//
LOCUS       DAPCMU010000284         1299 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPCMU010000284 DAPCMU010000000
VERSION     DAPCMU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.294"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..969
                     /locus_tag="RD744_002887"
     CDS             <1..969
                     /locus_tag="RD744_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDU0558812.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="RD744_002888"
     tRNA            1159..1232
                     /locus_tag="RD744_002888"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          429 a          232 c          264 g          374 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagctt
//
LOCUS       DAPCMU010000285         1280 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPCMU010000285 DAPCMU010000000
VERSION     DAPCMU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.295"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..241
                     /locus_tag="RD744_002889"
     CDS             <1..241
                     /locus_tag="RD744_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDU0558813.1"
                     /translation="RQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLIL
                     DIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            246..1193
                     /locus_tag="RD744_002890"
     CDS             246..1193
                     /locus_tag="RD744_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDU0558814.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          420 a          184 c          267 g          409 t
ORIGIN      
        1 taggcaacag acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga
       61 atttctaaaa aaaagtaatt atatttttga tgggcaatgt gggataataa aaatgagaga
      121 ttatctaatt ttagatattg atagtgaaga agattttaaa tggttagaat ttttgtttcc
      181 taaattttta gaagattctt cggagcttca agatatctat aattatttat atcggaggta
      241 aaaggatgag ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca
      301 atcatttagg agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag
      361 aaaatcttga tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct
      421 tgatggaaga gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg
      481 ctagtgtggc agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg
      541 tattaatgat tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta
      601 gttcttcagc ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga
      661 ttcgcccatt gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt
      721 attgtcattt atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa
      781 atcaaaatcc gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga
      841 aacaaatcgt aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag
      901 actttgttta tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg
      961 cactggggaa acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc
     1021 atgcaatcaa tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg
     1081 gtgatatcca tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga
     1141 aatatggtat tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac
     1201 taagataaga ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta
     1261 aaatcaaatc tgggatagaa
//
LOCUS       DAPCMU010000286         1253 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPCMU010000286 DAPCMU010000000
VERSION     DAPCMU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.296"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            112..>1253
                     /locus_tag="RD744_002891"
     CDS             112..>1253
                     /locus_tag="RD744_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDU0558815.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP"
BASE COUNT          396 a          160 c          205 g          492 t
ORIGIN      
        1 aaaaagaaac attgacaaat attgttcttt tctgtgaaca ttataaccgt ctatcagaag
       61 aaaaaatttt actcccagaa aactgggcag aattaagaaa gttggtagag tatgggatta
      121 tttaacacac tttccaagtc aaaattagtt aagtccagtt tctggtatac tatagggaat
      181 ttttttatta agggaatttc attcataact attcctattt ttgtaaatat tactactcaa
      241 gcagaatatg gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt
      301 gggctaagcc taaattcggc aattaataat gcaagttttg actataaaga tgacataaat
      361 gaatttatgt catcggtact ttctctagct acactttcgt taattgtgtt cttattaata
      421 gggaacatat attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat
      481 ttgatgttat tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg
      541 attaacttta accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt
      601 gcaatctctg tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta
      661 gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata
      721 aagggtgctc atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg
      781 ctaatcccac attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat
      841 tcttatttag gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt
      901 ctaagcgtaa tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg
      961 agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact
     1021 ttgattacga tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa
     1081 gactattatt ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt
     1141 ttatatagtt taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt
     1201 ggtacagtag caagtgctat agttaatata ctattaaaca tgattttcat acc
//
LOCUS       DAPCMU010000287         1247 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPCMU010000287 DAPCMU010000000
VERSION     DAPCMU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.297"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            161..1201
                     /locus_tag="RD744_002892"
     CDS             161..1201
                     /locus_tag="RD744_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDU0558816.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          502 a          178 c          208 g          359 t
ORIGIN      
        1 actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa
       61 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
      121 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      181 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      241 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      301 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      361 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      421 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      481 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      541 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      601 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      661 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      721 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      781 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      841 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
      901 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtggtat
      961 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
     1021 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1081 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1141 tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact ggttagagtg
     1201 aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaa
//
LOCUS       DAPCMU010000288         1245 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPCMU010000288 DAPCMU010000000
VERSION     DAPCMU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.298"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(247..1107)
                     /locus_tag="RD744_002893"
     CDS             complement(247..1107)
                     /locus_tag="RD744_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558817.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          376 a          237 c          182 g          450 t
ORIGIN      
        1 aagggaaaca aacaacacat ttttgtgtgt tgcttatttc cctctaacta tcgttttcta
       61 tttgatggtt aacctactta aaataaatac tgcaacgaca aacggagatc aagcatgctt
      121 tgtaacgtat tttacgataa ttacgacttt attttattac tctatcttgc tgccataccc
      181 gtctatccct aatcatcttt ggtgaatttt ttcaaagtta gcataacaaa aataagtatt
      241 ttttccttag ttaatcaata gtatcatttt tttcatatga tttaattcat caattaattt
      301 tttgttttta ttaattttag caaaagctag tgcatcttga atcaattcga gcacttctgc
      361 cttcgactta tttttagcct gtgcgttctt cgctaattgg gcagcagctc gtgctaagta
      421 ataagtcaaa tggttatccg aacaaatatc gattgcgtaa tttagtagtg catcacttgt
      481 ttccaaatca ccgtcttgcg caaaaaaaac accacaatga aagacgatat ttaacacccg
      541 ccagatatct tctgttgtac gaatcggata cgtataaatt ttttcaaaca ctcgattgaa
      601 ataaacttct gctttttcca actctttttc tctagcatag accattccaa tacctgtata
      661 tgccaataaa cgaaacaacg tgttggctgt ttctgccatc aacaactgat caaaatggaa
      721 caacacatcc gtaattggtg cattcgtaaa aatcatttca aatcctttta gatagtagta
      781 acgtgcaatc agctcagact cgtcaattac cttgggatca atggctgcta aaatctgtgt
      841 caattgttca tattcactcg taatcagtga aaactctgct ttttctagct gttcatcaag
      901 ttgtgtattg atcacgttca tcgaagaaaa aagttcaact aacggaagat tgattcgatt
      961 acataatttg attaaaattt ttaacgaagg gacttgacca ttgttctcaa atttacttaa
     1021 tgttgcttgt gtacaaattc cttcagacac ttctttttga gataagttgg cagcctttct
     1081 tgccgtaata aatttttcaa tgttcattat tttctctcca atcagacctc acacgaaaat
     1141 catttctgtc tcgattttag catgttttct ttgggaatgc tttaggttct gttgcaaagt
     1201 tttaaataaa gaataaagtc ccttacggta tctatgattt aagct
//
LOCUS       DAPCMU010000289         1232 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPCMU010000289 DAPCMU010000000
VERSION     DAPCMU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.299"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(153..980)
                     /locus_tag="RD744_002894"
     CDS             complement(153..980)
                     /locus_tag="RD744_002894"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDU0558818.1"
                     /translation="MVLDFIEILKVIFLGIVEGITEWLPISSTGHMILADKFITLNMS
                     EAFKEMFFVVIQLGAILAVVVMFWNKMFPFQLKDKSQPLVKKDTFSLWFKVAVACIPS
                     GIMGILFDDYLDAYLQTPIVISIMLIFYGLLFILIENWNKKRTPTTMALSDISYKTAI
                     LIGAFQVLSLIPGTSRSGATIIGALLIGVSRVAAAEFTFFLAVPTMLGASAFKLLKFG
                     FDFTSAELLALVLGMAVAFAVSVLVIKFLMNFIKKHDFKVFGWYRIVLGILVYLLYL"
     gene            complement(980..>1232)
                     /locus_tag="RD744_002895"
     CDS             complement(980..>1232)
                     /locus_tag="RD744_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacitracin ABC transporter permease"
                     /protein_id="HDU0558819.1"
                     /translation="FVAMKTKGFVAPMIGSAVIVMGSAALSNQEWGALYPWTATYFLV
                     QGKLQSTGYPTLLSVSIIILVSAVGFLMTFHHFKKEDLK"
BASE COUNT          421 a          259 c          217 g          335 t
ORIGIN      
        1 ttggtttgtg taaacgcaaa gaaacggcgg ctctgaaaat caaagccacc gtttcatagg
       61 cgtgaaaata tgtctgctgt acgacggctt ttttcttttg ccgtcgtgcg gcagattatt
      121 gcttatggaa ttgtttttct atcacattac tgttataagt acaacaagta taccaagata
      181 ccaagcacaa ttcgatacca accaaacacc ttaaaatcat gttttttgat aaagttcatt
      241 aggaatttaa ttactaagac agataccgca aatgccacag ccataccgag aacaagagcg
      301 agcagttctg cacttgtaaa atcaaacccg aattttaaca gcttaaaagc acttgctcca
      361 agcatagtag gtactgcgag gaaaaaagta aactctgctg ctgccactcg tgaaactcct
      421 atgagtaaag cacctataat cgttgctccc gaccgtgatg tgccgggtat aagtgataac
      481 acttgaaatg cccctatcaa gattgctgtt ttatagctga tgtcagaaag tgccatagta
      541 gtaggagtac gctttttatt ccaattttct atgagaatga ataacaaacc ataaaagatt
      601 aacatgattg aaatcacaat gggggtttgg aggtaagcat ccaaataatc atcaaataaa
      661 atccccataa tacctgacgg tatacaagcc actgcaacct tgaaccacaa cgagaaagta
      721 tcctttttaa caagtggctg cgatttatcc tttagctgaa atggaaacat cttgttccaa
      781 aacattacaa ccactgcgag tatagctcca agttgaataa caacaaaaaa catttcttta
      841 aatgcttcgc tcatattaag tgtgataaat ttgtccgcaa gaatcatatg tcctgtgctg
      901 ctgataggta gccactcagt aataccctca acaattccaa gaaaaataac ttttaaaatt
      961 tctataaaat caagtaccat tatttcaaat cctccttttt aaaatgatga aaggtcataa
     1021 gaaaaccaac tgctgataca agaataataa tagatacaga cagcagtgta ggatagccgg
     1081 tactctgaag tttaccctgc accaagaaat aggtggctgt ccacggatac aacgctcccc
     1141 attcttgatt tgaaagtgcg gcacttccca tgacaatcac ggcagagcca atcatcgggg
     1201 cgacaaatcc ttttgttttc atagcaacaa ac
//
LOCUS       DAPCMU010000290         1226 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPCMU010000290 DAPCMU010000000
VERSION     DAPCMU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.300"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..467
                     /locus_tag="RD744_002896"
     CDS             <1..467
                     /locus_tag="RD744_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDU0558820.1"
                     /translation="KTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFV
                     SVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPK
                     FMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            476..703
                     /locus_tag="RD744_002897"
     CDS             476..703
                     /locus_tag="RD744_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDU0558821.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          381 a          196 c          204 g          445 t
ORIGIN      
        1 ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg
       61 gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga
      121 ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt
      181 tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa
      241 acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac
      301 ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa
      361 tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc
      421 ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc
      481 aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag
      541 tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc
      601 catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt
      661 gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac
      721 agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct
      781 attattcaga ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg
      841 actcatacaa agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt
      901 ttcctgttac gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac
      961 tgatagcagt ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac
     1021 cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt
     1081 ttagtaagct cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa
     1141 catttactat gtagttttga taaaatgatt tgaataacag tgatggacaa ttctttttta
     1201 tttttaacat tattttgata ataatg
//
LOCUS       DAPCMU010000291         1214 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPCMU010000291 DAPCMU010000000
VERSION     DAPCMU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.301"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            70..1026
                     /locus_tag="RD744_002898"
     CDS             70..1026
                     /locus_tag="RD744_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558822.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="RD744_002899"
     CDS             complement(1080..>1214)
                     /locus_tag="RD744_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558823.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAPCMU010000292         1185 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPCMU010000292 DAPCMU010000000
VERSION     DAPCMU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.303"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..331
                     /locus_tag="RD744_002900"
     CDS             <1..331
                     /locus_tag="RD744_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDU0558824.1"
                     /translation="ESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILA
                     DEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVN
                     KMNLEVE"
     gene            331..>1185
                     /locus_tag="RD744_002901"
     CDS             331..>1185
                     /locus_tag="RD744_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0558825.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSN"
BASE COUNT          503 a          124 c          220 g          338 t
ORIGIN      
        1 tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa atgttatgaa
       61 attatctggt ggacagcaac aaagagtagc tattgctaga gcattggtat cagatgctcc
      121 aataatacta gctgatgagc ctactggtaa tctagacagt gttactgctg gagaaataat
      181 taatatatta aagacattag ctaaagatag aaataaatgt gtaatagttg taacacatag
      241 taaggaagta gcagattctg cggatatcat tttagaacta agtggtaaaa agttgaaaaa
      301 agtaaataaa atgaatttgg aggttgaata atgataaaaa atgcatttgc ttatgtaact
      361 agaaaaagtt taaaatcatt aattatcata ttagttattt tatctatgtc aactttaagt
      421 ctcattagtt tatctattaa agatgctacg gacagagctt caaaagaaac atttgctaat
      481 ataacaaata gtttttctat ggagataaat agacaagtaa atccaggaac acctagaggt
      541 gggggaaatg taaaaggtga agatattaaa aagatatctc aaacagatag tatagactcc
      601 tatgtaaaaa gaataaacag tgttgcagat ttagttgatt atgatattat tgaaactcaa
      661 gagactcttg cgaatcaatc acctgaaaga gcaaagaatt ttaaaagaac agttatgtta
      721 actggggtta acgactcaac aaaagaaaca aaatttgtat cagaagcata taaattagta
      781 gaaggaaagc atttagaaaa tgaagataaa aataaaatct taatgcataa agatttagct
      841 gaaaaaaata accttaaagt tggagataag ataaaaataa aatctaattt atttgatgct
      901 gataatgaga aaggtgcaga tgaaacagta gaagtagaaa ttaaaggtct attcgatggg
      961 cataatagcg gtggagtaag tgcagcacaa gaactatacg aaaatacatt aatcactgat
     1021 attcatacag ctgctaaagt ttatggtaat accgaagata cagctgtata ccaagatgca
     1081 acattctttg taaaaggtga taagaatctt gatagtgtaa taaaagatct tgggaaatta
     1141 gatataaatt ggagagaata taatttgatt aaaagttcat caaat
//
LOCUS       DAPCMU010000293         1180 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPCMU010000293 DAPCMU010000000
VERSION     DAPCMU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.304"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            157..942
                     /locus_tag="RD744_002902"
     CDS             157..942
                     /locus_tag="RD744_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HDU0558826.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            935..1177
                     /locus_tag="RD744_002903"
     CDS             935..1177
                     /locus_tag="RD744_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558827.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          456 a          182 c          183 g          359 t
ORIGIN      
        1 ctaaataatt tttgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa
       61 gttgctatat accctcttat tttagcatac tcccttttag aaaaagcaaa aaaaaaagaa
      121 aaaatcttat tcttgataac aagctacgtg acgtatatgc ctaacaaaaa tagaactttt
      181 gaatcaatct taaatgaagg tttaaccaca gaaccggcat ccattatttt tgatgaagtt
      241 atcgatgacg ttgaagatag taagtatcat aaaaatttaa aagaactttt ttattttgca
      301 ctcaacaatg gaccaagttt gttcactggt atatcagtac ctgatagtga taatccatta
      361 attatggcaa aaatttactt aacaaaatgg tgtaataaat acatcaggga taggaataac
      421 ccagcattaa aaaaacctct taaaacattt ggcgaaaaag atgctgcttt aactactaga
      481 gtagcagcaa atgctaatat tgatgatcaa aaagtattga atgactactt gcgtggacat
      541 tttttatata tgagcgctga gaacatgaac ggaagtattt tagaggaata tttagccgaa
      601 gtgttagaac ctgaaggttg gatttggtgc gccggctctg tatatcgagc tgttgatttc
      661 tgttatttag gaacctcacc aattctttta caagtgaaaa ataaatacaa tacagaaagc
      721 agttcgagtt cagctataag agtaggaaca acaattagaa aatggaatcg tttaaataag
      781 tcgacaaaaa tttctggttt ggatagccct atccccaatt ggaaagcatt aatagaaatg
      841 actgaagcta gtaaggaact ggcagctaag ttaactgaaa atagttattt agcttatatt
      901 aatgaaaaat ccacacgaga attatggaca ctcgatgact aacaatatcc ttattagtat
      961 ctatccacga tacatggaaa aaattataaa caaacaaaaa aactttgaat ttagaaattt
     1021 ctatcttacc tcagatcctt ataatatctt ttggatttac gaaacaatcc ccaccaaagc
     1081 tttaaagtat aaattgattg taaaaaatcc aattactaaa cttaagaaaa atcaacacta
     1141 ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAPCMU010000294         1164 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPCMU010000294 DAPCMU010000000
VERSION     DAPCMU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.305"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..626)
                     /locus_tag="RD744_002904"
     CDS             complement(<1..626)
                     /locus_tag="RD744_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558828.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1164
                     /locus_tag="RD744_002905"
     CDS             914..>1164
                     /locus_tag="RD744_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDU0558829.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEE"
BASE COUNT          382 a          189 c          223 g          370 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aata
//
LOCUS       DAPCMU010000295         1163 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPCMU010000295 DAPCMU010000000
VERSION     DAPCMU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.306"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(257..1129)
                     /locus_tag="RD744_002906"
     CDS             complement(257..1129)
                     /locus_tag="RD744_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDU0558830.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          318 a          278 c          225 g          342 t
ORIGIN      
        1 atttgagtgt aacataaatg gcttttttat ttgtctcgct taattataca aacggcgtgt
       61 aaaaaactat tgcacaatac aagtggccta cggctatcga aatttccata actttcgagc
      121 aagaatttat ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc
      181 tgcctaactg gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg
      241 aagagccttt tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga
      301 gcgttccagc gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt
      361 cgacataccc attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt
      421 gtttcatcac accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt
      481 aggcacattg agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct
      541 cttgtccttt tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac
      601 agttgccatg aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca
      661 tatgccccat ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc
      721 cgccaacacc cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga
      781 agacatactc accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa
      841 attctttttt catggtaccg atgaaatcaa aattttgcca tgccaatttc ggggtcgagg
      901 taatgtaccc ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc
      961 caatccctgc taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggcattgttt
     1021 cctcaggtgt cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgccaa
     1081 tggcacacac aaattttgtg ccgcctgctt caattgcacc gtatagcata gacatttctc
     1141 cctttatttt agaatgaaat cac
//
LOCUS       DAPCMU010000296         1157 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPCMU010000296 DAPCMU010000000
VERSION     DAPCMU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.307"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>1157
                     /locus_tag="RD744_002907"
     CDS             <1..>1157
                     /locus_tag="RD744_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDU0558831.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF"
BASE COUNT          379 a          229 c          226 g          323 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggattt
//
LOCUS       DAPCMU010000297         1150 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPCMU010000297 DAPCMU010000000
VERSION     DAPCMU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.308"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            73..>1150
                     /locus_tag="RD744_002908"
     CDS             73..>1150
                     /locus_tag="RD744_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDU0558832.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT          365 a          215 c          229 g          341 t
ORIGIN      
        1 agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt tataatagat
       61 ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac gaatggtaga
      121 aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct cgttcgaaag
      181 attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac ttactccgat
      241 ttaggtcgcc caagtatcga tccagtgatt ctgatcaaat tggtgtttat tcagtattta
      301 ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc
      361 tggttcttag gctactcctt cgaggagaaa atcccccatt tctcaacttt tggaaagaat
      421 tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta tattttagaa
      481 caagcagtta aagcaggttt tgtaacagag gataatttgt atctggattc tacccacatc
      541 aaagctaacg ccaataaaca taagtttacg aaagaaatga cccatgatga agcgaaagcc
      601 tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg aaaacgccct
      661 tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag taaagctgat
      721 ccggaaagcg gctactacgt caaaggcgaa cgagaaaaac aatttgctta ttctgcccat
      781 acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg aaatattcat
      841 gacagtcaag tcgcctttca gctcgtcaaa caatcgaaga gactttttcc tgaaatcaat
      901 tgtgttgtcg ctgatgctgg gtataagaca cctaagtttg ttcacttttt gactcatttg
      961 aaccttcgac cttgtttgcc ttattcaagg cccaaaggga aaaaggggct attatcaaaa
     1021 aatgagtttc tctatgacga gtattttgat tgttatattt gtccgcaaga tcaaatgctc
     1081 gctttttcaa cggttactcg agaagggtac cgagaataca aatccaatcc aaaagaatgt
     1141 gtgaactgtc
//
LOCUS       DAPCMU010000298         1143 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPCMU010000298 DAPCMU010000000
VERSION     DAPCMU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.309"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="RD744_002909"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="RD744_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDU0558833.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAPCMU010000299         1133 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPCMU010000299 DAPCMU010000000
VERSION     DAPCMU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.310"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(88..1014)
                     /gene="cysK"
                     /locus_tag="RD744_002910"
     CDS             complement(88..1014)
                     /gene="cysK"
                     /locus_tag="RD744_002910"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDU0558834.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT          301 a          267 c          177 g          388 t
ORIGIN      
        1 ataatttttc tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata
       61 aacctatctg gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata
      121 acgttcacca ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc
      181 gacttttagc gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt
      241 tcgcccaact tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc
      301 atacacagaa gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg
      361 aggtcccgct tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc
      421 gattttcgga tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc
      481 gatacctgca acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc
      541 agtcgtcttc tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta
      601 gccgttttct tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc
      661 aggagtcaaa atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat
      721 cgtctcaggc atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc
      781 gattcctgta tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc
      841 gtgttctgct ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt
      901 aaaaaactca agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt
      961 gacgattggc gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa
     1021 ttcctccttg tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt
     1081 gattcgtctt tgactagcaa aaaaaagaca aagatatacc atatatggta att
//
LOCUS       DAPCMU010000300         1120 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPCMU010000300 DAPCMU010000000
VERSION     DAPCMU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.311"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            36..446
                     /gene="spx"
                     /locus_tag="RD744_002911"
     CDS             36..446
                     /gene="spx"
                     /locus_tag="RD744_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDU0558835.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            460..816
                     /locus_tag="RD744_002912"
     CDS             460..816
                     /locus_tag="RD744_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDU0558836.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          394 a          163 c          215 g          348 t
ORIGIN      
        1 gtagaattgg attataatta aaaatgagtg agaaaatgat aaaagtatat acttcgccta
       61 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      121 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga
      181 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      241 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      301 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      361 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      421 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      481 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      541 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      601 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      661 attgcgattg acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat
      721 accaatctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      781 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      841 ctgagcattt catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      901 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
      961 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1021 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1081 ttgttgacat gttagaaatg acatgataag ataactgagt
//
LOCUS       DAPCMU010000301         1116 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPCMU010000301 DAPCMU010000000
VERSION     DAPCMU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.312"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="RD744_002913"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="RD744_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDU0558837.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DAPCMU010000302         1104 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPCMU010000302 DAPCMU010000000
VERSION     DAPCMU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.313"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            73..234
                     /locus_tag="RD744_002914"
     CDS             73..234
                     /locus_tag="RD744_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDU0558838.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>1104
                     /locus_tag="RD744_002915"
     CDS             253..>1104
                     /locus_tag="RD744_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0558839.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIK"
BASE COUNT          465 a           99 c          191 g          349 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaa
//
LOCUS       DAPCMU010000303         1077 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPCMU010000303 DAPCMU010000000
VERSION     DAPCMU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.315"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>1077)
                     /locus_tag="RD744_002916"
     CDS             complement(<1..>1077)
                     /locus_tag="RD744_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0558840.1"
                     /translation="FFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRNGS
                     YTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMEL
                     YENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMS
                     FICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFP
                     NAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTW
                     EPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLK
                     TISSTLPQSFKKKFTIFHCYQSGIS"
BASE COUNT          308 a          206 c          225 g          338 t
ORIGIN      
        1 gaaatacccg attggtagca atggaagatc gtgaatttct ttttaaacga ctgaggtaac
       61 gtagatgaaa ttgttttaag ggtttcttga aagccttccg tacctcgttt aacgaaataa
      121 tagcgtaata attgacaaaa atgatacgca gttcgcaatt cttcgttgta gcttaacagc
      181 tcatcaacga tttctgtttg actaattggt cgtttaaaca gtcgatgata atgccgtttg
      241 agtggctccc atgtcgtgga atctttgagt aagagtttcc aatatcgctt gagccgacgg
      301 tacttcttct gattttcaga gtgtgaggtt cgaaaacgat tcatgatctg gacacgtaat
      361 tgattgaagc tgcgattgat gtgttggaca atatgaaagc ggtcagttac gatctccgca
      421 tttggaaaga ctgttttaag caactgacaa tagctggcat tcatgtccat cactaggtac
      481 ttgaccttta aacgggcttt tctaggaaag tgcaggaagt attgagttag tttgtaaagt
      541 ctgcgatctt caaggatcgt aaatagctga tgggttgtcc catcaacaca aataaaactc
      601 atggaaccag aaacagactt cattgatttg aactcatcca tacacaacac cgtcggaagg
      661 aagttcatac gtgactggta atgcttcgtt gcttcacgca agatccgtgt caccgtccca
      721 tccgagacga agtacttctt agcgattagt ttacgtgatt gattttcgta cagttccatg
      781 agaattgcct gcttcagttc ctttgttagt tgatgatgtt catcaactaa gtccgttttc
      841 gcagaaaagg tccttccaca atcatggcag agaaaacggg tacgttttaa ttctaaaata
      901 gttaactttt ctttgacttt caatatctga gtgcgtgtcg tataggaacc gttgcggata
      961 atacgaaccg atcgacagtg ttcacaatag gaaggagagt catccaattt tcctttaata
     1021 aactgcgctt gaacgccttt atattctttt tcaatcagcc attcttcatc aaaaaat
//
LOCUS       DAPCMU010000304         1044 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPCMU010000304 DAPCMU010000000
VERSION     DAPCMU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.316"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(232..780)
                     /gene="aac(6')"
                     /locus_tag="RD744_002917"
     CDS             complement(232..780)
                     /gene="aac(6')"
                     /locus_tag="RD744_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDU0558841.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          312 a          216 c          153 g          363 t
ORIGIN      
        1 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
       61 atttttctta ctactcgaaa ctaagcgact ttttcacaac ctctggtaca cggtgttcca
      121 aaagtagctt cttcggaaat aagttggaat ttcacaaaaa ttcaagaagt aattttcgta
      181 aattccttct tatttcttga agctgattac ttctgtcaca acctcttttt attattgaga
      241 atttggtcga ggaataatcg tttttgccat ccaaatatcc ggtttgtccc agccatttgc
      301 atttggtaag acacctacga ttttataacc taatttttca tagaattcat acggatgttc
      361 acgaaggttc tggatggaag ccactttatc aaatgtatgc tcatacaggt ccgtttgact
      421 taacgttgtt ccatggtcta aatcatccgt acctaaataa atcgtgattc ctcctctgga
      481 agctacttct ttttctaagt aattgactaa tcgagtacct atttggttct ttcgtcggga
      541 gctttctaca actaatggat gcaattccca acctgtgata ccgtattgag ggattgcacc
      601 aataaatcct actaactcat cttggtctac cgctgctacc gcgattcgtt ctggattcat
      661 catttcttct acttcttctg ccgagctgtc tccatattct tccggccaag tcagtctcag
      721 taaatcagaa agctgatctt tcaatactgg attattacgg tcaaattcac tgattatcat
      781 acgtcttccc ccttgttctt gcttattcta acagtccttt tgaaaaaacg aaatgttctc
      841 gctcaaaaat ctttagctac caatcagtat ctctattctt tcattattta tcttacttat
      901 aaaataaaaa aattatacag aaacacttta ttcaaaatag gaaagatcag gaaagccatc
      961 aattggagga aaaaactctg tttgttcatc atgatataga ttatcttttg ctttaaaaaa
     1021 aaccaattat gaattgacaa ataa
//
LOCUS       DAPCMU010000305         1035 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPCMU010000305 DAPCMU010000000
VERSION     DAPCMU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.317"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            98..838
                     /locus_tag="RD744_002918"
     CDS             98..838
                     /locus_tag="RD744_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558842.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          381 a          184 c          200 g          270 t
ORIGIN      
        1 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
       61 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
      121 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
      181 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
      241 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
      301 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
      361 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
      421 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
      481 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
      541 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
      601 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
      661 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
      721 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
      781 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
      841 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
      901 tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt
      961 ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt tcgcagatcg
     1021 tttgcaaaaa tagtt
//
LOCUS       DAPCMU010000306         1033 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPCMU010000306 DAPCMU010000000
VERSION     DAPCMU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.318"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            400..1033
                     /locus_tag="RD744_002919"
                     /pseudo
     CDS             400..1033
                     /locus_tag="RD744_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          356 a          183 c          186 g          308 t
ORIGIN      
        1 ataaaatatt ttttatatta caaaagtagt gtttcaaagt tagtagagaa aatgtagtta
       61 tatatttaaa tttttaccag aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg
      121 gttagatgta atcatcagga aaaacaatct taaacgaatt actattcatg gtcttagaca
      181 cacacatgct agcttgcttt tagcaagcta gtgcaaatat taaagatgtg caagagcgtt
      241 taggccactc ttctattcaa atcattatgg atctatatat tcatattaca gacaaaagaa
      301 aagaagaaac cgtagcgcaa ttcgcaaaat ataggctcta taatttatgg gactgatgaa
      361 tcaaaattga ttcttcagtc ccattgttat tttaggtata tgattcgtac attgaaagag
      421 ttgaaaaact atcttccgaa gccgtcaaaa aggaccctgc caaaagtatt gatggtggat
      481 gaatttcgtt cgcacgcttc aattgtggat aaaatgagct ttatttgtgc ggatggcgag
      541 actggaaaac tcattgatat tttgcctaca cgaaaattgt ctcgattaac acactatttt
      601 agtcgctgtg caaatcctga agcggtggaa tatttagtga cagatatgaa cgccgcctac
      661 ttccaactga caaaacgtgt cttaccaaat gcgaaagtcg tcattgattg ttttcatatc
      721 atcaaacatt tgaatcaggc gttcaatgat tttcgtgtac aagaaatgaa aagattacac
      781 cagttagggc aacgacggga agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa
      841 aaccgtcaaa aaatcaatca ctatgattac aaaacatggc gcagttttcg tgcacctaaa
      901 tacccgctac tcactgaagc aatgatgatt gatcgattgc tggcattttc aacgccttta
      961 aaggacgcct atactttttt ccatgagctg accgaagcct ttagagacaa ggaatcagac
     1021 ttatttttta cct
//
LOCUS       DAPCMU010000307         1017 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPCMU010000307 DAPCMU010000000
VERSION     DAPCMU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.319"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="RD744_002920"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="RD744_002920"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="RD744_002921"
     tRNA            203..276
                     /locus_tag="RD744_002921"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="RD744_002922"
     tRNA            316..405
                     /locus_tag="RD744_002922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="RD744_002923"
     tRNA            420..491
                     /locus_tag="RD744_002923"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="RD744_002924"
     tRNA            507..579
                     /locus_tag="RD744_002924"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="RD744_002925"
     tRNA            589..661
                     /locus_tag="RD744_002925"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="RD744_002926"
     tRNA            697..772
                     /locus_tag="RD744_002926"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="RD744_002927"
     tRNA            774..854
                     /locus_tag="RD744_002927"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="RD744_002928"
     tRNA            861..934
                     /locus_tag="RD744_002928"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="RD744_002929"
     tRNA            937..1009
                     /locus_tag="RD744_002929"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          241 a          228 c          272 g          276 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttat
//
LOCUS       DAPCMU010000308         1016 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPCMU010000308 DAPCMU010000000
VERSION     DAPCMU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.320"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            1..331
                     /locus_tag="RD744_002930"
                     /pseudo
     CDS             1..331
                     /locus_tag="RD744_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            365..>1016
                     /locus_tag="RD744_002931"
     CDS             365..>1016
                     /locus_tag="RD744_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HDU0558843.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFL"
BASE COUNT          366 a          118 c          189 g          343 t
ORIGIN      
        1 accaattatt gattcgacag gtctatgttt attagatgaa ttcttggcgg atatcagtct
       61 atttataatg tttattgatg taattgaaga aacatcaaga aattttttgc tgtctaagtt
      121 cagaaaattt cgtatgaaaa atgataaaca aaaattattg gcaaatgaga tttggagtag
      181 tttagaagtc gaagaaagta aacaagaact tttgttgaaa tggatacttt ttcttgtaaa
      241 gaagttctat gtgaaaatac cagaaaccca ttgtctgata gttatgcacg gtaaaataac
      301 agcttctgcg atcgcaaggg aaacaaataa gttgctgaat acatatgttt atgaggcttt
      361 cgatatgcca attgaaggtg agactagtga cttaattaac ctaatcaaca atttttgtaa
      421 aagtatagat acgacgaatg gactcattct gctagttgat atgggatcat tggaacaaat
      481 gtatgaaaaa attgaatcca atgtaatagg tgatttggta attttaaaca atgtttctac
      541 agctttagcg attgaatgtg ggattcaagt ttgccaaaaa aagccaatta gtcattttta
      601 ccagatggat tttgattcgt ttcaagttaa agttcagtat tataaaggat tatcacaaaa
      661 gaaaaatgta atagtatctt gtctctctgg tgaggggatt tctgaaaaag ttaaggatat
      721 tttaaaacgg tatctcgata atcaagtgga gattttgatt tttgatttta acgcattaaa
      781 gaaaatagca aaggaaaaag atactatgat ttttaagaat acaatttgtg ttctttcaac
      841 cactgaattt tacattcaag gaatcgattg tttaaattta gaaaatctaa ttaatgggaa
      901 tcaaacctta gaaaaattaa ataagtatat ggactcggat agctgtgagt tctgcttaaa
      961 tgaattagtt aaactcttta ctattgaagg cgctagtact aagcttcgct ttttag
//
LOCUS       DAPCMU010000309         1014 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPCMU010000309 DAPCMU010000000
VERSION     DAPCMU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.321"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>1014
                     /locus_tag="RD744_002932"
     rRNA            <1..>1014
                     /locus_tag="RD744_002932"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          272 a          229 c          310 g          203 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgt
//
LOCUS       DAPCMU010000310         1010 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPCMU010000310 DAPCMU010000000
VERSION     DAPCMU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.322"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..848
                     /locus_tag="RD744_002933"
     CDS             <1..848
                     /locus_tag="RD744_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0558844.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          284 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt
//
LOCUS       DAPCMU010000311          996 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPCMU010000311 DAPCMU010000000
VERSION     DAPCMU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.323"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(25..912)
                     /locus_tag="RD744_002934"
     CDS             complement(25..912)
                     /locus_tag="RD744_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDU0558845.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          322 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaatt
//
LOCUS       DAPCMU010000312          994 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPCMU010000312 DAPCMU010000000
VERSION     DAPCMU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.324"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>994)
                     /locus_tag="RD744_002935"
     rRNA            complement(<1..>994)
                     /locus_tag="RD744_002935"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAPCMU010000313          985 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPCMU010000313 DAPCMU010000000
VERSION     DAPCMU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.325"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..704
                     /locus_tag="RD744_002936"
     CDS             <1..704
                     /locus_tag="RD744_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0558846.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataa
//
LOCUS       DAPCMU010000314          983 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPCMU010000314 DAPCMU010000000
VERSION     DAPCMU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.326"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            154..>983
                     /locus_tag="RD744_002937"
     CDS             154..>983
                     /locus_tag="RD744_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0558847.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          344 a          144 c          167 g          328 t
ORIGIN      
        1 cttattatct ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat
       61 tttgtgtaaa tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt
      121 accacaaccg aaattttata ggaggacttt ctcatgccca attttactac agaaattatg
      181 gaaacactaa tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct
      241 gttaattcat tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt
      301 gctgggttca attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag
      361 tatggagaat taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact
      421 ttacctgctt ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa
      481 aaaggaatta ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat
      541 acgccacaaa ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa
      601 gaaaggtcct tagaatccag atactctgtc atttttatgg atgctactca cattccttta
      661 aaaagacaaa ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga
      721 accaaagagg ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag
      781 attcttcaag acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt
      841 ttaagtggca ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt
      901 tgtgtccata tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc
      961 tgtagcgatt tcaaattagt tta
//
LOCUS       DAPCMU010000315          959 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPCMU010000315 DAPCMU010000000
VERSION     DAPCMU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.327"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(107..511)
                     /locus_tag="RD744_002938"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="RD744_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="RD744_002939"
     CDS             complement(570..>959)
                     /locus_tag="RD744_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558848.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAPCMU010000316          950 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPCMU010000316 DAPCMU010000000
VERSION     DAPCMU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.328"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            175..408
                     /locus_tag="RD744_002940"
     CDS             175..408
                     /locus_tag="RD744_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDU0558849.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            405..812
                     /locus_tag="RD744_002941"
     CDS             405..812
                     /locus_tag="RD744_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDU0558850.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          365 a          127 c          170 g          288 t
ORIGIN      
        1 atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt
       61 aattatatag aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt
      121 taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca
      181 attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag
      241 tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt
      301 cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa
      361 gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc
      421 tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg
      481 aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct
      541 cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac
      601 aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt
      661 ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt
      721 tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca
      781 acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt
      841 tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa
      901 ctatatagaa aacgtaaatt ttctatatag ttggattaag ataacgacta
//
LOCUS       DAPCMU010000317          942 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPCMU010000317 DAPCMU010000000
VERSION     DAPCMU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.329"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(61..279)
                     /locus_tag="RD744_002942"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="RD744_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="RD744_002943"
     CDS             complement(545..739)
                     /locus_tag="RD744_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558851.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="RD744_002944"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="RD744_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          321 a          160 c          149 g          312 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ct
//
LOCUS       DAPCMU010000318          931 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPCMU010000318 DAPCMU010000000
VERSION     DAPCMU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.330"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>931
                     /locus_tag="RD744_002945"
     CDS             <1..>931
                     /locus_tag="RD744_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDU0558852.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAPCMU010000319          911 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPCMU010000319 DAPCMU010000000
VERSION     DAPCMU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.331"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            6..>911
                     /gene="bsh"
                     /locus_tag="RD744_002946"
     CDS             6..>911
                     /gene="bsh"
                     /locus_tag="RD744_002946"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDU0558853.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKE"
BASE COUNT          314 a          122 c          166 g          309 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga a
//
LOCUS       DAPCMU010000320          897 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPCMU010000320 DAPCMU010000000
VERSION     DAPCMU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.332"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            68..>897
                     /locus_tag="RD744_002947"
     CDS             68..>897
                     /locus_tag="RD744_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDU0558854.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          313 a          134 c          164 g          286 t
ORIGIN      
        1 gaaaaaagga agtcccttct gtagaataga gttaccacaa cacattcaca gaaaagagga
       61 cttccatatg aacgatttta ctacagaaat tatggaaata ctaatcaata aaggtgattt
      121 ggatgatttg tttcgtcgtc acctagaatt ggctattaac gctttattac aagctgaatt
      181 aacggtattt cttgactatg aaaagtatga tagagctgga tttaattcag gtaattcccg
      241 taatgggaat tattcgcgct cttttaaaac agagtacggt gaattgaatt tagtaatacc
      301 tagagatagg aatggagact tttctcaaca aactttacct gcttataaaa gaaccaatga
      361 ctctttagaa accactatta ttcagctagt tcaaaaagga attactatgt ccgaaatctc
      421 tgatctaatc gaaaaaatgt atggtcatta ttacaaacca caaactattt caaatatgag
      481 taaaattgtg tctgaagatg tttcggcatt taaagaaaga actttagaag ctaaatactc
      541 agtcattttt atggatgcta ctcatattcc cttaaaaaga caaaccgtat caaaagaagc
      601 tgtctatata gtaattggta tcaggttgga tggaactaaa gaggttctag gatttactat
      661 agctccaact gaatctgctt atgtttggaa agagattctt caagatctaa aagaccgtgg
      721 tttagaagag gttttgttag tcgtaacaga tggtttaagt ggtattgacg atagtattca
      781 tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc catatatcta gaaatatcgc
      841 tcataaggtt cgtgttagtg atcgacaaga aatctgtagc gatttcaaat tagttta
//
LOCUS       DAPCMU010000321          887 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPCMU010000321 DAPCMU010000000
VERSION     DAPCMU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.334"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(544..744)
                     /locus_tag="RD744_002948"
     CDS             complement(544..744)
                     /locus_tag="RD744_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0558855.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          283 a          170 c          115 g          319 t
ORIGIN      
        1 taaatcactc tacaataacg gcgaaagcac aaaaaataaa aaaattatta cgttattaaa
       61 aaaacagatg aaagatcagg ttttattaga tctcattctt tcatcacttg aagggattta
      121 atgaactact ttttcctctt tgtcgttttt atatattcgt attccttcaa acagtaggat
      181 agcctctttc aatgcttgat atttgaactg tccgttactt aaaaatttag atgtgccgcc
      241 ctcttcctag aacatattct tttttacttt ttcattaatt gacagaccaa tcgaacgaaa
      301 ctgtaaattt cgataggata atgattggtc aaactcattc attttcaaga catctcgatt
      361 catattcaag ataggaatct ttttatcaac atcactttat tcgtattttc ctctaccata
      421 aaagcttcac gctccacaaa attgttttat acactactat tcagcccatt caacgaccaa
      481 tgaatttttt cgtacaaaaa aaccgtcttt tctttcgaaa agacggttaa aagtcaattt
      541 ttattaagct ttaacaatgt ttgttgcttg agggccacgt tgaccatctt caacatcgta
      601 agacactgct tggccttctt ctaaagtttt gaagccgtca ccttggatag ctgaaaaatg
      661 tgcaaataca tcatttccat cttcaccagt gataaagcca aaacctttgt ctgcgttgaa
      721 ccattttact gtaccgttat tcataaataa attcctcctg atacgtatca atgtacgttt
      781 tttcgttgca ataaatattt gataggcaaa aggagtttta tctatgtaga tatatcgctc
      841 aaattacgtt tcaaaataga ttactcattt aatatatcat gcttttt
//
LOCUS       DAPCMU010000322          863 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPCMU010000322 DAPCMU010000000
VERSION     DAPCMU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.335"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            86..355
                     /locus_tag="RD744_002949"
     CDS             86..355
                     /locus_tag="RD744_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDU0558856.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="RD744_002950"
     CDS             348..605
                     /locus_tag="RD744_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDU0558857.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          329 a           95 c          164 g          275 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta cta
//
LOCUS       DAPCMU010000323          863 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPCMU010000323 DAPCMU010000000
VERSION     DAPCMU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.336"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            32..>863
                     /locus_tag="RD744_002951"
     CDS             32..>863
                     /locus_tag="RD744_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558858.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          298 a          151 c          166 g          248 t
ORIGIN      
        1 ccgaccaaag aattataagg agtgagtaca aatgacctat acccatctta caacggatga
       61 acttgtaata atagagtctt atttcaaaat gaatcaatct gttgctaaaa ctgcccattg
      121 cttgaatcgt tcaagacaaa cgatccataa agtatatctg ttcttcaagc aaggaaaatc
      181 agccttagaa tattatcaac agtataagaa aaacaaatca aactgtggta gacgtccgct
      241 tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag gatggacacc
      301 cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta ccatttatcg
      361 tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca agcgtaaacc
      421 gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc atgaacgtga
      481 aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg
      541 tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag ttatcatcac
      601 attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc aatggtttga
      661 atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg aattttcaaa
      721 ttggaaacag atcagtaatg tcaatgatat tgccatttat ttcgctgatc caggaacgcc
      781 gtctcaaaga ggcctaaacg agaattctaa cggattgtta cgtagagatg gtttattgaa
      841 atctatggat ttcaattcag tag
//
LOCUS       DAPCMU010000324          856 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPCMU010000324 DAPCMU010000000
VERSION     DAPCMU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.337"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            179..763
                     /locus_tag="RD744_002952"
     CDS             179..763
                     /locus_tag="RD744_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDU0558859.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          272 a          124 c          174 g          286 t
ORIGIN      
        1 ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt tctttttttg
       61 ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa tatctatgtt
      121 cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag tgattttttt
      181 gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac aagaagacag
      241 cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg caaaaagtaa
      301 tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac ttgtcgtttg
      361 gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca atcgactgaa
      421 tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca aatcaagttc
      481 tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc gtaatttgat
      541 cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt ttggtggtcg
      601 tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg atagcggtac
      661 acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt atcgttattt
      721 ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt catttaatgc
      781 tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt ttgtaagtgt
      841 ctactgataa cttatt
//
LOCUS       DAPCMU010000325          840 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPCMU010000325 DAPCMU010000000
VERSION     DAPCMU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.338"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            136..309
                     /locus_tag="RD744_002953"
     CDS             136..309
                     /locus_tag="RD744_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558860.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          280 a          141 c          104 g          315 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
//
LOCUS       DAPCMU010000326          835 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPCMU010000326 DAPCMU010000000
VERSION     DAPCMU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.339"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            39..656
                     /locus_tag="RD744_002954"
     CDS             39..656
                     /locus_tag="RD744_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558861.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          319 a          107 c          153 g          256 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgtt
//
LOCUS       DAPCMU010000327          822 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPCMU010000327 DAPCMU010000000
VERSION     DAPCMU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.340"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            367..555
                     /locus_tag="RD744_002955"
     CDS             367..555
                     /locus_tag="RD744_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558862.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="RD744_002956"
     CDS             545..805
                     /locus_tag="RD744_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558863.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
BASE COUNT          253 a          126 c          183 g          260 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt tt
//
LOCUS       DAPCMU010000328          811 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPCMU010000328 DAPCMU010000000
VERSION     DAPCMU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.341"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..672
                     /locus_tag="RD744_002957"
     CDS             <1..672
                     /locus_tag="RD744_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558864.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAPCMU010000329          787 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPCMU010000329 DAPCMU010000000
VERSION     DAPCMU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.342"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            357..>787
                     /locus_tag="RD744_002958"
     CDS             357..>787
                     /locus_tag="RD744_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDU0558865.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLD"
BASE COUNT          264 a          147 c          127 g          249 t
ORIGIN      
        1 ccgaatgcaa aacagaagtt ctataagtta taatcatagt tgttaggtga ttcatctagt
       61 aaaaacacca gatttcaaat ttcacacaat ttaaactaga tcaatcatca accttcttca
      121 aattgttaga accctttccc gaggagctgc agcataagca aaaaaacaaa caggaagcca
      181 aatggctaag acattttttc tattagtcta gttgtttctc gtcgcaagtt taagacttta
      241 gaaagttatt tctaatgatg tttgggttgt tttgatcaat gcttatgcac agctccttac
      301 ctattattta tctcaacaga tctaacaaat taatggacta tttaaggagt ttttttatgc
      361 ttacgatcta ttcttctcgc acacatagtt accatctgat ggcaaactgg ctgaaagacc
      421 atcaaatgcc ttacagagaa cgaattttca cacctaaaca gcctttaaca aggaaagaga
      481 tattttattt attgtcatta acagaaaacg gatttgacga tctattagct aaacgctcga
      541 ttgattataa aaatttgaaa gaccaaatcg accaatgtac gacctcagag cttgtcgatc
      601 tgatcgtctc gcattttact ttgttacaac gtcccatcat tactgacgga aagaaattat
      661 tagtcggatt caatgaagat agcatgcgtc tttttatccc tgtacaatgg cgcagaagtc
      721 aactgaaatc tgtctataac gattttacat gggtaagcaa tctagaaaat tctacggatt
      781 tagatga
//
LOCUS       DAPCMU010000330          787 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPCMU010000330 DAPCMU010000000
VERSION     DAPCMU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.343"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            96..284
                     /locus_tag="RD744_002959"
     CDS             96..284
                     /locus_tag="RD744_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558866.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
BASE COUNT          285 a          121 c          149 g          232 t
ORIGIN      
        1 taaaaaacat ctattttatc gaagaaacac aaaatatcga gggttcttat attgaagtaa
       61 aaacactctt tgtcaatgaa gataaaacca aagcattgga tatttataaa aaattagctt
      121 caaaaaaaac gaatagtttt ggattgatat taagcgagta taaaatcaaa gcagaagaga
      181 gttatttcta tcaattatta aaacggtgga gtaagcttcc cgccgatttt tatcgaaaaa
      241 tgcagatcat caactatcaa ccgcttgctg agacacatgc ctaaatagag gttgtgaaat
      301 cagtgctttg agctaatacc gaaggtcagc tgattgaaca ttgtttagaa gagattgtga
      361 caactgcgat ttgctttgag taataagaac gctggacaga aaatagcttc tcatattttc
      421 tagtctagtg ggcttattgc cgagaagcag cagatggaac atcgtttaga agagattgtg
      481 acaactgcga tttgctttga gtaataagaa cgctggacag aaaatagctt ctcatatttt
      541 ctagtctagt ggacttattg ccgagaagca gcagatggaa catcgtttaa agaagagatc
      601 ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg tacctttgat
      661 tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt tttgccactc
      721 cccattctag aatcaacccg tatgatgtag ataatgaatt cacgttgaaa aaagatttct
      781 caaataa
//
LOCUS       DAPCMU010000331          782 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPCMU010000331 DAPCMU010000000
VERSION     DAPCMU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.344"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..293)
                     /locus_tag="RD744_002960"
     CDS             complement(<1..293)
                     /locus_tag="RD744_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558867.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRK"
BASE COUNT          262 a          134 c          130 g          256 t
ORIGIN      
        1 tcttttcgcc cgataatgac gtcaggcgtc caaccaagag tgactttctc tttaatgtaa
       61 tctacttcat gagcaggtaa ttgaatgact tttctaccac attttttctt attttcttta
      121 tactggtgcc aataatcaag agcagtcttg ccttgcttga acttattgac tacattataa
      181 atagtttgta tagcacgtcc cattcggtta gcgatttcaa ccggcttatt atgttgaaga
      241 taatatgatt ctatcattgt cagttcgtct atggtaagat gtttgtaggt catttatggt
      301 tacactcctt tgttttcttt cgtcggaaat acaatttgag tgtaccataa atgccttttt
      361 atttttctaa cttaatttta caattcgcga tatattaaac aacgttgtcg cgcttcattg
      421 aaacatttta tgaaaattcc aaaaaagagt tgacacatgt ttttagacgt gataagataa
      481 ctaagtcgtt aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa acttcaaaaa
      541 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
      601 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
      661 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctcccgatga agtagacctt
      721 tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc aattcaagac
      781 aa
//
LOCUS       DAPCMU010000332          781 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPCMU010000332 DAPCMU010000000
VERSION     DAPCMU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.345"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            219..>781
                     /locus_tag="RD744_002961"
     CDS             219..>781
                     /locus_tag="RD744_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558868.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSID
                     SELLSFGIGLTLLICLIILTFNFKKLG"
BASE COUNT          247 a          109 c          112 g          313 t
ORIGIN      
        1 catttcacat taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg
       61 aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc
      121 ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata
      181 ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac
      241 atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa
      301 tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac
      361 tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca
      421 tgactttatt aatttacact catgttcttt ctgtagctat gactatggta atgattataa
      481 ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta attattaaat
      541 taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt cctatgattg
      601 agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa aaaagagcaa
      661 tttatctggg taaagagttt ttgattggaa gtatagattc agaattacta tcatttggta
      721 ttggcttaac tttgcttatt tgtttaatta tactaacatt taactttaaa aaattaggaa
      781 t
//
LOCUS       DAPCMU010000333          767 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPCMU010000333 DAPCMU010000000
VERSION     DAPCMU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.346"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            54..734
                     /locus_tag="RD744_002962"
     CDS             54..734
                     /locus_tag="RD744_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISEnfa1 family transposase"
                     /protein_id="HDU0558869.1"
                     /translation="MTQFKGKQFQKDVIIVAVGYYLRYNLSYREVQEILYDRGIHVCH
                     TTIYRWVQEYGKILYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDVDGLTLDI
                     WLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKLQKNGLYITTEHRTIK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRGIYKKSRKEGSLFGFSVCTEI
                     KGLLGIPA"
BASE COUNT          284 a          133 c          152 g          198 t
ORIGIN      
        1 ataagctcat tttgaggtaa aataattgaa aaagatagct tggaggaatg aagatgactc
       61 agttcaaagg aaaacaattt caaaaagacg tgattatcgt agccgtcggc tactatcttc
      121 ggtacaacct gagttatcgt gaagtgcagg aaattctgta tgaccgagga attcacgttt
      181 gtcacaccac aatttaccgt tgggtgcaag aatacggaaa gattctttat caaatttgga
      241 aaaagaaaaa caaacagtcc ttttattcat ggaaaatgga cgagacttat atcaaaatta
      301 aaggaaagtg gcattattta tatcgcgcta tcgatgtaga tggcttaacc ttagatatct
      361 ggttacggaa aaagcgcgac acacaggctg cgtatgcttt tctaaaacga ctgaagaacc
      421 agtttggaga accaaaggtt ctagtaacgg ataaagcacc ctctattaag agtgccttca
      481 gaaagcttca gaaaaatgga ctgtatataa caacagaaca tcgaacaatc aagtatctga
      541 ataatctgat tgaacaagac catcgtccaa ttaagcgaag aaacaaattt taccaaagtt
      601 tgcgaacagc ctcaaccacg attaaaggca tggaagcgat tcgaggaatt tataaaaaaa
      661 gccgaaaaga agggtctctt tttggctttt ccgtctgtac agaaatcaaa ggactattag
      721 gaatccctgc ttaaataaga atcatcttga aaaactaatg acctttt
//
LOCUS       DAPCMU010000334          758 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPCMU010000334 DAPCMU010000000
VERSION     DAPCMU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.347"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            330..>758
                     /locus_tag="RD744_002963"
     CDS             330..>758
                     /locus_tag="RD744_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDU0558870.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKS"
BASE COUNT          288 a           84 c          125 g          261 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatct
//
LOCUS       DAPCMU010000335          746 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPCMU010000335 DAPCMU010000000
VERSION     DAPCMU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.348"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..686
                     /locus_tag="RD744_002964"
     CDS             <1..686
                     /locus_tag="RD744_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDU0558871.1"
                     /translation="AYIEILKWQDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFS
                     RADIACDIIDVPDEFITQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKL
                     EQETKRKVVPKEVETWWRLEMQLRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRI
                     MLAGLMSDQNFWSLLSRNSKYKYRELLKEESQNDELTNHLRETFAESADNLKQELDTW
                     LLGLDVTEK"
BASE COUNT          242 a          111 c          176 g          217 t
ORIGIN      
        1 ttgcttatat tgagattttg aaatggcaag atggcaaagg gcggattgat tttaacccga
       61 ataagattgg tcatttttta gctagttcaa tgaaaaattt cattcatgat ttatttctag
      121 aaccacattt ctcacgtgcg gatattgctt gcgatattat cgatgtgcct gatgagttta
      181 ttacacaata tcgagtggtt gatcctgttt ctttcaagcc aatttatggg cggtctggga
      241 agctggaaac agcttattgg ggcagtcgtg ccagcgaacg acaaattcgg ctatacaata
      301 aaaaattgga acaagaaacc aaacgaaaag ttgtaccaaa ggaagttgag acttggtggc
      361 ggttagaaat gcaactacgg cgtggcaagg caaccgattg gcataatatg gttcaagaaa
      421 gtttgaatag ttttgcgagc ctgcattttt tgccgattga tacaacacct acagatagaa
      481 taatgctcgc tggtttaatg tctgatcaaa atttctggtc tcttttgtcg aggaattcaa
      541 aatataaata tcgggaattg ctgaaagaag aaagtcaaaa tgatgaattg acgaatcatt
      601 tgcgagaaac gtttgcagaa tcggcagaca atttgaaaca agaattagac acgtggcttt
      661 tgggtttgga cgtgacagag aagtgatacc gaataagaaa agtcagatga gtgagagtaa
      721 atttacgtca atgtttaatg acgtga
//
LOCUS       DAPCMU010000336          733 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPCMU010000336 DAPCMU010000000
VERSION     DAPCMU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.349"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>733
                     /locus_tag="RD744_002965"
     rRNA            <1..>733
                     /locus_tag="RD744_002965"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAPCMU010000337          729 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPCMU010000337 DAPCMU010000000
VERSION     DAPCMU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.350"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(62..670)
                     /locus_tag="RD744_002966"
     CDS             complement(62..670)
                     /locus_tag="RD744_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDU0558872.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          184 a          136 c          141 g          268 t
ORIGIN      
        1 caaaaaaacc gagaacttga ataaacaagt ttctcggttt catcattttc cttttatcgg
       61 cttagtacca gccgtttgct aaccagaatg ttttagcagc atcccatgaa ccataacgag
      121 atgttacata ttgttctgct acacgttctt gattggcagc tgaatagtcg ccgtttaagt
      181 atgaagcatc taattgataa cggccgatgt aacgtccgtt agtagctgtg tatgaaccgc
      241 ctgattcttt ttgtgcgatc cattcttttg cagagcttga agttggcgct gttgcagcag
      301 ctggggcagc aggagctact tcaactgttt caacaggtgc aacgtatgta tgttcttgcg
      361 ttgctgtttc agttgtagat gctgtatttt ctgttgtttt gttttctgtt gttgctgaag
      421 aatcatttga agttgggatt gttaattgtt caccaacata aatgcggttg atgtttgcga
      481 tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc tcctgcaaat ttttgtgaga
      541 tcttagataa tgaatcacct gattttactg tataaacttc atcagcgtga gcatttgtag
      601 ttcctacaaa cattgcagta gcaccagcag caaaagttgt acctaaaaca attttcttaa
      661 atgaattcat attaaactcc tttttttctc cgttttttat tctttcgacg ctattaactt
      721 tatcattta
//
LOCUS       DAPCMU010000338          729 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPCMU010000338 DAPCMU010000000
VERSION     DAPCMU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.351"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..380
                     /locus_tag="RD744_002967"
     CDS             <1..380
                     /locus_tag="RD744_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558873.1"
                     /translation="EDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIE
                     DTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVV
                     KFNGTEIGRQIFDIKVNKPVEKN"
     gene            394..>729
                     /locus_tag="RD744_002968"
     CDS             394..>729
                     /locus_tag="RD744_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558874.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDT"
BASE COUNT          266 a          100 c          133 g          230 t
ORIGIN      
        1 cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta attaatccag
       61 ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta acagctgtgg
      121 tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt ttaaacaaga
      181 atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct caagttaacg
      241 atataaactt taatataaac gccagaaatg tcttggttga agaagcagga gagcattatg
      301 ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc aaggtcaaca
      361 aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc atcaaataca
      421 catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc ggtaattcta
      481 gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga taatcaactt
      541 gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa aatttttgat
      601 gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga tgaagattta
      661 aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga ttacgatgtt
      721 ttggatact
//
LOCUS       DAPCMU010000339          699 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPCMU010000339 DAPCMU010000000
VERSION     DAPCMU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.352"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            132..344
                     /locus_tag="RD744_002969"
     CDS             132..344
                     /locus_tag="RD744_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558875.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>699
                     /locus_tag="RD744_002970"
     CDS             506..>699
                     /locus_tag="RD744_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDU0558876.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDI"
BASE COUNT          249 a          104 c          122 g          224 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatt
//
LOCUS       DAPCMU010000340          698 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPCMU010000340 DAPCMU010000000
VERSION     DAPCMU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.353"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>698
                     /locus_tag="RD744_002971"
     CDS             <1..>698
                     /locus_tag="RD744_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDU0558877.1"
                     /translation="DFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYN
                     VIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSN
                     QRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIH
                     EKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESG
                     EKVRRRKLIANDIL"
BASE COUNT          259 a           94 c          122 g          223 t
ORIGIN      
        1 gattttgata tgattttact agtagcgtat aaaattttaa ctgtaaacag taacgttgca
       61 cgtaatattg ctagtaatat aagggcgatt tatgtggatg aatttcaaga tactagagaa
      121 ttacaataca atgtaatagc taaattactt caaaacaacc ctcaaattca gtcaatgttt
      181 gttggagata ttgaccaagc aatttatgga tcattagatg gtatagctaa atcagttgaa
      241 gaactagaaa tgctaactag tcacactttt caatcaaaga cgctacatgg ctgttacagg
      301 tctaatcaga gattagtaga cttctactct aattttcaga gttgtcctta cgagattgaa
      361 agcagaggga ataataaaaa cgaccgtggt tttataagtt atgattgcaa agttactaag
      421 gaggatttac ccaacataat aaaaaatata ataaaggaaa agattgaaag cggcattcat
      481 gaaaaagata tttgtgtact tgctcctcaa cactatcctc tgttttcact gggtgagtat
      541 ttaaagaaag aattaccgta ttgcaatttt gatgctataa atatatcacc aattaaggta
      601 aataatcata gtttattttt caagttagca attttgtact ttactgaatc aggtgaaaag
      661 gttcgaagaa gaaagctaat tgcaaacgat attttaat
//
LOCUS       DAPCMU010000341          696 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPCMU010000341 DAPCMU010000000
VERSION     DAPCMU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.354"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(54..629)
                     /locus_tag="RD744_002972"
     CDS             complement(54..629)
                     /locus_tag="RD744_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDU0558878.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAPCMU010000342          656 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPCMU010000342 DAPCMU010000000
VERSION     DAPCMU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.355"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..446)
                     /locus_tag="RD744_002973"
     CDS             complement(<1..446)
                     /locus_tag="RD744_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558879.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          194 a          117 c           89 g          256 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagt
//
LOCUS       DAPCMU010000343          651 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPCMU010000343 DAPCMU010000000
VERSION     DAPCMU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.356"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            26..>651
                     /locus_tag="RD744_002974"
     CDS             26..>651
                     /locus_tag="RD744_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDU0558880.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTL"
BASE COUNT          235 a          111 c          131 g          174 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg
      601 catggaagcc attcgaggat tatataagaa aacccgaaaa gaaggcactc t
//
LOCUS       DAPCMU010000344          635 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPCMU010000344 DAPCMU010000000
VERSION     DAPCMU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.357"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..270)
                     /locus_tag="RD744_002975"
     CDS             complement(<1..270)
                     /locus_tag="RD744_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558881.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="RD744_002976"
     CDS             complement(276..>635)
                     /locus_tag="RD744_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDU0558882.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAPCMU010000345          597 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPCMU010000345 DAPCMU010000000
VERSION     DAPCMU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.358"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          201 a           89 c          140 g          167 t
ORIGIN      
        1 actctttgtc aaagagtaac agaaaggttt acactctttt tattagcgac agcgcgaagg
       61 aggggtttaa cttggggttt agtatttcgt tcaaaaaggg aacaaaaaat acaagcattt
      121 ctcataataa tcgtgagtta actgatagcc aaaaaattaa tagtttgtct ggtagaaaat
      181 aattagcaga tttacaagtg ggaaataaaa agctgaaaga agaaaattgc atttcaaact
      241 acattttcta caataactct aaaatggcat attttgcgtt ctgagcggtt ttagcgttta
      301 tttcgtttag ttatcggcac aatcggtgaa acacgttaga ggagcgaaaa tggagcctgt
      361 aaggcgactg gtagcggaac ggtcgtgtta gattgtgtag ccgatagctg aacgaaataa
      421 atgctggcag cgccattcta tttcggcagg aggaggctca aggggttcgg ggaatgaaat
      481 tccctgaggt ctttttattt tttctagagg agtgctataa tcaaagaaaa tgagaaaaaa
      541 gagtaaaaaa atagtcgagc agtgttgacg cactctcgac ctttacggaa actaatc
//
LOCUS       DAPCMU010000346          595 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPCMU010000346 DAPCMU010000000
VERSION     DAPCMU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.359"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(131..553)
                     /locus_tag="RD744_002977"
     CDS             complement(131..553)
                     /locus_tag="RD744_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558883.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT          185 a          104 c           88 g          218 t
ORIGIN      
        1 ttttttgtgt ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat
       61 taattttact tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca
      121 taaaaaatcc ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag
      181 ctgatttata actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt
      241 tccaattccg tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc
      301 ctgtcactcg tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat
      361 tttcagtaac agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag
      421 gagttaattc ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg
      481 taggaattgt agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa
      541 acttattttt cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tctta
//
LOCUS       DAPCMU010000347          580 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPCMU010000347 DAPCMU010000000
VERSION     DAPCMU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.360"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            20..>580
                     /locus_tag="RD744_002978"
     CDS             20..>580
                     /locus_tag="RD744_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDU0558884.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIE"
BASE COUNT          173 a          157 c          146 g          104 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
       61 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
      121 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
      181 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
      241 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
      301 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
      361 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
      421 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
      481 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
      541 caaccgcccg aataaacggg tgctagggaa atccattgaa
//
LOCUS       DAPCMU010000348          540 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPCMU010000348 DAPCMU010000000
VERSION     DAPCMU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.361"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            417..>540
                     /locus_tag="RD744_002979"
     CDS             417..>540
                     /locus_tag="RD744_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0558885.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQ"
BASE COUNT          197 a           79 c           83 g          181 t
ORIGIN      
        1 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
       61 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
      121 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
      181 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
      241 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
      301 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
      361 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
      421 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
      481 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
//
LOCUS       DAPCMU010000349          538 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPCMU010000349 DAPCMU010000000
VERSION     DAPCMU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.362"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(18..371)
                     /gene="tnpB"
                     /locus_tag="RD744_002980"
     CDS             complement(18..371)
                     /gene="tnpB"
                     /locus_tag="RD744_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDU0558886.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          155 a          120 c           92 g          171 t
ORIGIN      
        1 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
       61 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      121 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      181 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      241 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      301 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      361 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      421 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      481 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DAPCMU010000350          514 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPCMU010000350 DAPCMU010000000
VERSION     DAPCMU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.363"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..132)
                     /locus_tag="RD744_002981"
     CDS             complement(<1..132)
                     /locus_tag="RD744_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDU0558887.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAPCMU010000351          510 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPCMU010000351 DAPCMU010000000
VERSION     DAPCMU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.364"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          228 a           72 c           82 g          128 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tacgatataa aagttgctac
      481 ggcagccaaa aacgaatcgg gaaacactct
//
LOCUS       DAPCMU010000352          496 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPCMU010000352 DAPCMU010000000
VERSION     DAPCMU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.365"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>496)
                     /locus_tag="RD744_002982"
     CDS             complement(<1..>496)
                     /locus_tag="RD744_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDU0558888.1"
                     /translation="VLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNY
                     AKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALK
                     VIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGII
                     AFIC"
BASE COUNT          171 a           91 c           61 g          173 t
ORIGIN      
        1 atacatataa aagcaattat accacttgat acaagtactt gaagaaatcc attatgcatg
       61 tttccatgtg ctctttttag ttcattcaca tctgtagtat caagatctac ttttttcaaa
      121 aattttgcat cgctattccc atagaaatcg gtctctccat aaccaaataa caaagttggt
      181 cttataactt taagcgcagc tttccaaatc gtaaaacgac cagctgaaat ttcggagtta
      241 tcactagaat gctgtaatat tacttctttt tctggaagtg gagttttatc tgattcattt
      301 gactctataa cttctacttt tctatcaatc gatacaggaa ttgttgctaa aaaatcctta
      361 gcataattag taccaaaaga aaaatatacc aatagaacta ataatatgct taccatccat
      421 ttgattttta caggtagtac ttctctaatg ctctttctaa attttgaatt ggcaaacaac
      481 aaaagaaata atacta
//
LOCUS       DAPCMU010000353          485 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPCMU010000353 DAPCMU010000000
VERSION     DAPCMU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.366"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(27..425)
                     /gene="spxA"
                     /locus_tag="RD744_002983"
     CDS             complement(27..425)
                     /gene="spxA"
                     /locus_tag="RD744_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDU0558889.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          140 a           98 c           84 g          163 t
ORIGIN      
        1 tcttttccaa gtttcgtatt atccttctat aatccagcca tcaattgtgc ttgacgtaat
       61 tctaattgtc gtacatcgcg cggtaggaac cgacgaattt catcttcatt aaaaccaact
      121 tgcagacgtt tatcatcaat catgattgga cggcgaagca atccaggatt gttttgcaca
      181 agttccaata attcttgtaa cggcagatca tctaaatcca tgttcagttt ttggaatact
      241 ttggaacgtg tcgagatgat ttcttctgtt ccgtcttctg tcatttgtaa aatcgctttt
      301 aattcagaac tgttcaatgg ttctgaaaaa atattacgtt caacaaatgg aatctgatgt
      361 tcttgcaacc acgcacgagc ctttctgcag gaagtacaac ttggggaagt atataatgtt
      421 aacaacgtgc accactcctt ttcgtgataa gaatcacttt tttgtaacag aagatcataa
      481 taatc
//
LOCUS       DAPCMU010000354          484 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPCMU010000354 DAPCMU010000000
VERSION     DAPCMU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.367"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(61..258)
                     /locus_tag="RD744_002984"
     CDS             complement(61..258)
                     /locus_tag="RD744_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0558890.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DAPCMU010000355          478 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPCMU010000355 DAPCMU010000000
VERSION     DAPCMU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.368"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            99..293
                     /locus_tag="RD744_002985"
     CDS             99..293
                     /locus_tag="RD744_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558891.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          203 a           61 c           82 g          132 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaa
//
LOCUS       DAPCMU010000356          445 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPCMU010000356 DAPCMU010000000
VERSION     DAPCMU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.369"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..>445)
                     /locus_tag="RD744_002986"
     CDS             complement(<1..>445)
                     /locus_tag="RD744_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDU0558892.1"
                     /translation="KNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDT
                     FKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPI
                     QTATMLQAKDYETKMYVMAVPKINSYLGTIERYETMYADDPMTARA"
BASE COUNT          112 a           89 c           80 g          164 t
ORIGIN      
        1 tgccctggct gtcattggat catctgcata catagtttca taccgttcaa ttgttcctaa
       61 atatgagttg attttaggta ctgccatgac atacattttt gtttcataat ctttggcttg
      121 aagcattgtt gcggtttgaa taggaacgtc tgttgttcgt cctgtacctt caatcactaa
      181 attataccct ttatctctca aacggcttat gatcgcttct gtcatgcgat tagaataagg
      241 ggtaacgtat tttactacgt ctttttcata aagtttcact agttcatcaa aattagggtg
      301 ctgttgtttg aacgtatcat tatcaatgat aacaacattt ccttgtgtct cttcggaaat
      361 tgccgatcgc aaactagttt tacctgatcc tggttgccca ccaagtaaaa aagcggttgg
      421 cgattcaacc gctagtctat tttta
//
LOCUS       DAPCMU010000357          439 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPCMU010000357 DAPCMU010000000
VERSION     DAPCMU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.370"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            2..157
                     /locus_tag="RD744_002987"
     CDS             2..157
                     /locus_tag="RD744_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDU0558893.1"
                     /translation="MYGGTTHSGKYYGNGVYCTKNKCTVDWAKATTCIAGMSIGGFLG
                     GAIPGKC"
     gene            159..>439
                     /locus_tag="RD744_002988"
     CDS             159..>439
                     /locus_tag="RD744_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDU0558894.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYR"
BASE COUNT          169 a           45 c           85 g          140 t
ORIGIN      
        1 tatctatggg ggtaccactc atagtggaaa atattatgga aatggagtgt attgcactaa
       61 aaataaatgt acggtcgatt gggccaaggc aactacttgt attgcaggaa tgtctatagg
      121 tggtttttta ggtggagcaa ttccagggaa gtgctaaaat gaaaaaaaat gctaagcaaa
      181 ttgttcatga attatataat gatatatcta taagtaaaga tcctaaatat tctgatattc
      241 ttgaggtttt acaaaaggta tatttaaaat tagaaaaaca aaaatatgaa ttagatcccg
      301 gtcctttaat aaatagattg gtgaattatc tatattttac tgcttatact aataaaataa
      361 gattcactga atatcaagag gaattaataa gaaatttgag tgaaattgga agaactgctg
      421 gaataaatgg tttatatcg
//
LOCUS       DAPCMU010000358          422 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPCMU010000358 DAPCMU010000000
VERSION     DAPCMU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.371"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            205..>422
                     /locus_tag="RD744_002989"
     CDS             205..>422
                     /locus_tag="RD744_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558895.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHY"
BASE COUNT          166 a           47 c           83 g          126 t
ORIGIN      
        1 ataagtgaag ataaagtaat tggtgtaatt aattgtagag aatagccttt gagcaattga
       61 agcgatacaa ttatttagaa aatgaacttc ctccagcata ctacatgatt ccttacggga
      121 cgatgttaaa gaagaaagca agaaatttta gaagtattag tagataaact aatgggcttt
      181 tctcaattta agggagtagc ccaaatgttt atcaaatatg gtgcagatac agtagcaacc
      241 atgtttacag aagaatatca agtagactat tatgaagtaa tagataaatg tgtacatttt
      301 gtttatgaaa aagtgtggaa ttttatgaat tttgcgataa gtgaagaaat cttacgagta
      361 caagaaaaca gaacaggaat atttaccaga gacagaaagc ttaatttgat tgcgcattat
      421 at
//
LOCUS       DAPCMU010000359          417 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPCMU010000359 DAPCMU010000000
VERSION     DAPCMU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.372"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..308
                     /locus_tag="RD744_002990"
     CDS             <1..308
                     /locus_tag="RD744_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558896.1"
                     /translation="IRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLI
                     EGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          157 a           67 c           58 g          135 t
ORIGIN      
        1 aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca
       61 tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt
      121 caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc
      181 aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc
      241 aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa
      301 tgttttaact tgatcaacag aatgggcttt tccatttaca cataattatt gacacaacct
      361 attctggaaa cttacaaata aaagcttatt cttcagccac cttcgggtgg ttatttt
//
LOCUS       DAPCMU010000360          415 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPCMU010000360 DAPCMU010000000
VERSION     DAPCMU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.373"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..339)
                     /locus_tag="RD744_002991"
     CDS             complement(<1..339)
                     /locus_tag="RD744_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558897.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAPCMU010000361          399 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPCMU010000361 DAPCMU010000000
VERSION     DAPCMU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.374"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
BASE COUNT          117 a           71 c           53 g          158 t
ORIGIN      
        1 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
       61 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
      121 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
      181 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
      241 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
      301 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
      361 gtattaaata atttaatttt tttgttccta tttatttaa
//
LOCUS       DAPCMU010000362          396 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPCMU010000362 DAPCMU010000000
VERSION     DAPCMU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.375"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>396
                     /locus_tag="RD744_002992"
     CDS             <1..>396
                     /locus_tag="RD744_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HDU0558898.1"
                     /translation="LTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSY
                     FYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEK
                     LALKGITISVPPRKNMDKSEKLDHSLLGKQ"
BASE COUNT          147 a           77 c           66 g          106 t
ORIGIN      
        1 ctcaccaaag gtgaactagt cggaattata gatagttttc ctagtccatt atgtaaacca
       61 gttagaaata gacaagcaaa actattgaat caaatagcca aagttggcta taactcaaca
      121 aaaaaatctt atttttatgg tcttaaaatt catatgatcg ttactaagac tggctttccg
      181 attacttact caatcacgaa tccaggtgtc catgacgtga aagtgttaga gactttatcg
      241 gaagaagcga atcttcccaa tatcctaggg gacaaaggat atattagcca taaaatccat
      301 gaaaagcttg cccttaaggg cattactata tccgttccgc cacgtaaaaa catggataaa
      361 tcagaaaaac tagaccacag cttacttgga aaacaa
//
LOCUS       DAPCMU010000363          361 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPCMU010000363 DAPCMU010000000
VERSION     DAPCMU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.376"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(76..312)
                     /locus_tag="RD744_002993"
                     /pseudo
     CDS             complement(76..312)
                     /locus_tag="RD744_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          115 a           55 c           64 g          127 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaataac gccgtttgta taattaagcg agacaaataa aaaagccatt tatgttacac
      361 t
//
LOCUS       DAPCMU010000364          360 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPCMU010000364 DAPCMU010000000
VERSION     DAPCMU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.377"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>360
                     /locus_tag="RD744_002994"
     CDS             <1..>360
                     /locus_tag="RD744_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HDU0558899.1"
                     /translation="WVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAI
                     DADGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLY
                     TKTEHRTVKYLNNLIEQ"
BASE COUNT          122 a           66 c           76 g           96 t
ORIGIN      
        1 ttgggtgcaa gagtacagca aagtcctcta ttatctttgg aagaagaaaa atagacaatc
       61 cttctattca tggaaaatgg acgaaaccta tatcaaaatt aagggacgtt ggcattatct
      121 ttatcgtgca attgatgcgg acggcttaac cttagatatc tggttacgaa agaaacggga
      181 tacgcaagca gcctatgctt tcttaaaacg actccataaa cagtttggtg agccgaaagc
      241 aattgtgacc gataaagcac cttctcttgg ctccgccttt agaaagttac agagtgtggg
      301 tttatatact aagacagagc accgaactgt gaagtatctt aacaatttaa tagaacaaga
//
LOCUS       DAPCMU010000365          357 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPCMU010000365 DAPCMU010000000
VERSION     DAPCMU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.378"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            71..>357
                     /locus_tag="RD744_002995"
     CDS             71..>357
                     /locus_tag="RD744_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558900.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
BASE COUNT          126 a           58 c           74 g           99 t
ORIGIN      
        1 ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg
       61 tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc
      121 tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat
      181 agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca
      241 aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat
      301 tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaa
//
LOCUS       DAPCMU010000366          343 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPCMU010000366 DAPCMU010000000
VERSION     DAPCMU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.379"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..321)
                     /locus_tag="RD744_002996"
     CDS             complement(<1..321)
                     /locus_tag="RD744_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDU0558901.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           96 a           55 c           63 g          129 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tga
//
LOCUS       DAPCMU010000367          341 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPCMU010000367 DAPCMU010000000
VERSION     DAPCMU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.380"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..>341
                     /locus_tag="RD744_002997"
     CDS             <1..>341
                     /locus_tag="RD744_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558902.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAPCMU010000368          339 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPCMU010000368 DAPCMU010000000
VERSION     DAPCMU010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.381"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..322
                     /locus_tag="RD744_002998"
     CDS             <1..322
                     /locus_tag="RD744_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558903.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
BASE COUNT          126 a           32 c           65 g          116 t
ORIGIN      
        1 ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat gggaagataa
       61 cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc tatttcttga
      121 tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga ttgaaactga
      181 agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt tttatttaag
      241 ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac cataccctat
      301 aacgcattta gtaaaaaaat aattcatgaa tggtttatt
//
LOCUS       DAPCMU010000369          338 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPCMU010000369 DAPCMU010000000
VERSION     DAPCMU010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.382"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            1..338
                     /locus_tag="RD744_002999"
                     /pseudo
     CDS             1..338
                     /locus_tag="RD744_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DAPCMU010000370          321 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPCMU010000370 DAPCMU010000000
VERSION     DAPCMU010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.383"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(69..321)
                     /locus_tag="RD744_003000"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="RD744_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAPCMU010000371          305 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPCMU010000371 DAPCMU010000000
VERSION     DAPCMU010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.385"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="RD744_003001"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="RD744_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDU0558904.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAPCMU010000372          299 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPCMU010000372 DAPCMU010000000
VERSION     DAPCMU010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.386"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            179..>299
                     /locus_tag="RD744_003002"
     CDS             179..>299
                     /locus_tag="RD744_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDU0558905.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRR"
BASE COUNT          116 a           47 c           45 g           91 t
ORIGIN      
        1 tattttgagt gtagcacaaa tgactttttt agttgtctag cttaatttta caatcggcgt
       61 aataaaaacc cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt
      121 aaatcaacaa aaaaacgcat atagtagtct caaaactccc aagaaatgag gactacatat
      181 gcgttcatca aaatatactg aacattatac agatactttt ataacattca aggaaattat
      241 gagaactgtt tctaacatat accacaattg tgtaccagat aagattaaaa accgaagaa
//
LOCUS       DAPCMU010000373          298 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPCMU010000373 DAPCMU010000000
VERSION     DAPCMU010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.denovo.387"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..298
                     /locus_tag="RD744_003003"
     rRNA            <1..298
                     /locus_tag="RD744_003003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           81 a           54 c           91 g           72 t
ORIGIN      
        1 agacagttcg gtccctatcc gtcgcgggcg ttggaaattt gagaggagct gtccttagta
       61 cgagaggacc gggatggact taccgctggt gtaccagttg ttctgccaag ggcattgctg
      121 ggtagctatg tagggaaggg ataaacgctg aaagcatcta agtgtgaagc ccacctcaag
      181 atgagatttc ccatttcttt aagaaagtaa gatccctgag agatgatcag gtagataggt
      241 caggagtgga agtacagtga tgtatggagc ggactgatac taatcgatcg aggactta
//
LOCUS       DAPCMU010000374        20791 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.040, whole genome shotgun sequence.
ACCESSION   DAPCMU010000374 DAPCMU010000000
VERSION     DAPCMU010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.040"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..251
                     /locus_tag="RD744_003004"
     CDS             <1..251
                     /locus_tag="RD744_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDU0558906.1"
                     /translation="LEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDN
                     LIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            486..722
                     /locus_tag="RD744_003005"
     CDS             486..722
                     /locus_tag="RD744_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558907.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            729..2681
                     /locus_tag="RD744_003006"
     CDS             729..2681
                     /locus_tag="RD744_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDU0558908.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2753..3250)
                     /gene="dfrG"
                     /locus_tag="RD744_003007"
     CDS             complement(2753..3250)
                     /gene="dfrG"
                     /locus_tag="RD744_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDU0558909.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3612..4652
                     /locus_tag="RD744_003008"
     CDS             3612..4652
                     /locus_tag="RD744_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDU0558910.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4750..4938
                     /locus_tag="RD744_003009"
     CDS             4750..4938
                     /locus_tag="RD744_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDU0558911.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4996..5349)
                     /locus_tag="RD744_003010"
     CDS             complement(4996..5349)
                     /locus_tag="RD744_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558912.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5880..6302
                     /locus_tag="RD744_003011"
     CDS             5880..6302
                     /locus_tag="RD744_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDU0558913.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6299..6529
                     /locus_tag="RD744_003012"
     CDS             6299..6529
                     /locus_tag="RD744_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDU0558914.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7027..7227
                     /locus_tag="RD744_003013"
     CDS             7027..7227
                     /locus_tag="RD744_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDU0558915.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7254..8447
                     /locus_tag="RD744_003014"
     CDS             7254..8447
                     /locus_tag="RD744_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDU0558916.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8515..10080)
                     /gene="guaA"
                     /locus_tag="RD744_003015"
     CDS             complement(8515..10080)
                     /gene="guaA"
                     /locus_tag="RD744_003015"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDU0558917.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10139..11062)
                     /locus_tag="RD744_003016"
     CDS             complement(10139..11062)
                     /locus_tag="RD744_003016"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDU0558918.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11258..11863
                     /locus_tag="RD744_003017"
     CDS             11258..11863
                     /locus_tag="RD744_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDU0558919.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12171..13187
                     /locus_tag="RD744_003018"
     CDS             12171..13187
                     /locus_tag="RD744_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDU0558920.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13328..14629
                     /locus_tag="RD744_003019"
     CDS             13328..14629
                     /locus_tag="RD744_003019"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDU0558921.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14675..15337
                     /gene="deoC"
                     /locus_tag="RD744_003020"
     CDS             14675..15337
                     /gene="deoC"
                     /locus_tag="RD744_003020"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDU0558922.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15358..15756
                     /locus_tag="RD744_003021"
     CDS             15358..15756
                     /locus_tag="RD744_003021"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDU0558923.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15822..16904
                     /locus_tag="RD744_003022"
     CDS             15822..16904
                     /locus_tag="RD744_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDU0558924.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17116..18678
                     /locus_tag="RD744_003023"
     CDS             17116..18678
                     /locus_tag="RD744_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDU0558925.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18671..19804
                     /locus_tag="RD744_003024"
     CDS             18671..19804
                     /locus_tag="RD744_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0558926.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19804..20760
                     /locus_tag="RD744_003025"
     CDS             19804..20760
                     /locus_tag="RD744_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDU0558927.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
BASE COUNT         6780 a         3685 c         4267 g         6059 t
ORIGIN      
        1 ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa
       61 gcataagatt tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata
      121 tagggattga taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag
      181 gtccgtctga actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc
      241 ttgcatatta agtagttcag ttttggagta caaaaagtga aaatgtaaac ctaaatgagt
      301 atcaaattgc aactgaattt gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa
      361 tttacaccac aaaatgtaag ttaaaatgta tataaaaatg cgaacgtgaa ataattattg
      421 taggaatgca aattggtgtg ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt
      481 ttcctatgca atttcttgag ccaaaaaata agaacgctaa gtctgtggat tgggaaatat
      541 cagagcaagt aagggtgata gttaagcaat acgctgaata tgcagagcgt actgaaagcg
      601 aagcagtgga tgaattctta ttgaacattt tagacgataa gaagtttatt gaatggattg
      661 ccaacaagcg aagtaataag cggattgttg aaaaaatggg tatcaaagat agagtgggtt
      721 gatagacaat gaccaagctt aaaagattag ccacgaagga agatgaaatt gtagatgtta
      781 aaattccgat ttcaaacgaa gaattaaaag atagggctaa acaatatgat ttattaactc
      841 caaaacgatt tgcaacaagg tataacaaaa tgttgttctt gccaacgtct tttaaatgga
      901 acggttctga atatcccatt caatataatt actgcatcaa tccgttttgt tgtaattttg
      961 ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc aagtcgatat aaaatgactg
     1021 gtagttccaa agataaaggt cataaaggta tgtactgcaa tgacaatcct attggcagag
     1081 gtgtatcgca aaactgtaca gtaacccctt tatctaattg gtctgttgtt gaagaaatta
     1141 aacgccttat agaaatcaat agcatccaag atgttgagcc agattatcaa tttcataaag
     1201 aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg
     1261 ggaaaagtag gggcaaatct caacgctatc aatgtaaggc gtgtaagaag ttcactaatg
     1321 ttctaccaaa aagagaagaa accactactt atcaccaaca aaagaacacc attcttccaa
     1381 tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg tacttgtgat attttaggaa
     1441 ttggagttgg aacctactat cataagctgg agtggctgta taggcgttgt ttggagtttt
     1501 tagaacgata tgagacacaa ccccttcaaa caaagaaatt taacgagatg tggcttaata
     1561 cggacaagat gcactactac ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat
     1621 atacggggtt tgaagacctt aatatgcaaa cgtatatagt tgtatctgct gaggtcttat
     1681 caagatatgt gttccgttct gatgttgcct atgattggaa tatttcaatg gatgaattaa
     1741 atgaagatac aagaaaattc aaagaagacc atctcaatac ttttagtcgt aaaaatgata
     1801 ggttagattg gtcttattat ccacaagaac catccgctaa cgatagcgaa aataggaatg
     1861 cttatcttca cgaattaggg aaaattacta ataggagtag atttgtcgat ggtttaaatg
     1921 ttgatgctcc ttatactaca actgctcact attggttaat taaacaaatg gttaatgccg
     1981 acgaatggcg aatgattagt gatgatgact tctctattag aaacgccttt tatagagtat
     2041 tcacgaaaga attaaggttg agcgatgcac atcattttat ttgccaagtt aacaagacga
     2101 aaagcagaaa acaatgctta aaagaattcg gacaagcaaa agcagaactg ctcgattggg
     2161 gagatatcag aggattcaaa acaaagtttc tacggacttt ggcatctcat tatctaactg
     2221 aattactaac aagtcatcaa ttccacgaag aggctattag taaagatgga gaaagatatc
     2281 gcaaatatgc cgataaccct atcaaacatc cattagccac taaggataaa ggattttatt
     2341 cagttgattg tagaacggat ttatctgctc tggaaccaaa cgaaatcgca aagatgctat
     2401 taaatgttaa tgaccattcg accaatagct ttattcaaca gataagacgt tatatatcat
     2461 ctcttgaaag accattaaca acagctcgtg gtgataagaa gagctatatt tacgctaact
     2521 tcaatccgaa gtatgctcaa tttgcgatta caattctaag aacttactac aacttttgtc
     2581 gcccttttaa atcagcggat aaaaaggttt tgacacctgc tcaaagatta gggattacag
     2641 ataaacaatt tgattggaaa gatataattt atttcaaatg acaaaaagag cttggagtaa
     2701 tcccaagctc ttgtgcttta tctttactgt tcttcttcaa ttaacgcacc cgttaactca
     2761 ataagttttt tctttcatat acatgaaaat agtagttata cggattttta tcattcttta
     2821 tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga aaaaaagtat
     2881 ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa gggaaaaaca
     2941 aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta cataactcaa
     3001 aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc ttatctctcg
     3061 tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg ttcttcctac
     3121 ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc ttgggaatcc
     3181 tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca gcaatcaaag
     3241 aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc ttatttctat
     3301 caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt tctataagtt
     3361 atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt caattgcatt
     3421 ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag tagtccattt
     3481 tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca tatatacgcc
     3541 tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa
     3601 aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt
     3661 ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata
     3721 caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg
     3781 ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag
     3841 atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt
     3901 tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg
     3961 agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat
     4021 ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat
     4081 caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa
     4141 atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa caaggattat
     4201 atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac tatagccctg
     4261 ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa gcctttagaa
     4321 aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca ccacaggaat
     4381 atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta aatactcaac
     4441 tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt caagattatc
     4501 gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag ctaaaaggat
     4561 atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt cggatagata
     4621 aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat ataaatatgg
     4681 tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag tggaaaggag
     4741 gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa taaaacaaga
     4801 ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc caaatgtaag
     4861 aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga gccagacgcc
     4921 aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc tttttgttta
     4981 tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt ttgctagtgc
     5041 ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta cctgtaacct
     5101 tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag atacatggag
     5161 taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt tttctatatt
     5221 tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag tgagaccccg
     5281 tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt tatcttcttt
     5341 tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca taaacaggta
     5401 tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat ttattaatgc
     5461 gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca actttataaa
     5521 atttagtcat actcctagaa agtagataaa attggcgact taatggaggg aaaagtcgcc
     5581 atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa aaaacttatc
     5641 cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata gatagaaaaa
     5701 tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca cttaataaat
     5761 atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg gggtttagta
     5821 tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg gtgaaaagca
     5881 tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc tgcaaacaag
     5941 ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca gcaaaagaag
     6001 tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt gatacgtacg
     6061 tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa agtgatttat
     6121 taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta cttcattact
     6181 ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt tcgactgttt
     6241 atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag tgtaaagaat
     6301 gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag atggcgacgt
     6361 ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac gttcattacg
     6421 tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc tacgtggcag
     6481 aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt cccatttttt
     6541 cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt tattccatca
     6601 aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt ctttgacaac
     6661 caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta acacgccatg
     6721 acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt agcaggattg
     6781 ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc cgagcgttag
     6841 aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa attcctgtga
     6901 gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta ttattataaa
     6961 actatttgat ataattatag gtgaatattg gagttttcct agcctataat tgggaaagga
     7021 gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg aacttctttt
     7081 tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca tatctactat
     7141 caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct aggaattcgg
     7201 ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa actatggtta
     7261 aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat ctaggttatg
     7321 attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa aaagaagcaa
     7381 gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag aataaggata
     7441 aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag acacaggtaa
     7501 agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct tattttgata
     7561 aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta aaattatcaa
     7621 agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt gctatggatc
     7681 gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc aatgtgaaaa
     7741 agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta atctctctaa
     7801 tttacaaaga agattactat caacactttt tatttatctc tctgtggttt ttatttatga
     7861 ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat tttgactcag
     7921 gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat agatttgttg
     7981 aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcaca ttaacttatt
     8041 tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta atgagctata
     8101 acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat gctaagctgg
     8161 ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc ttgttaatca
     8221 gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat attgagacga
     8281 ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct aacaaattaa
     8341 atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca aagaatcaat
     8401 ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa
     8461 agataagaag agccagaaac ccttggtata agaggatttc tagctctcct acaactattc
     8521 ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc
     8581 tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa
     8641 gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt
     8701 acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca
     8761 gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg
     8821 tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc
     8881 agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg
     8941 gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg
     9001 cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat
     9061 aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt
     9121 tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc
     9181 tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga ctttgatgat
     9241 gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag
     9301 cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac
     9361 cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac
     9421 ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc
     9481 acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg
     9541 tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat
     9601 cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc
     9661 accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc
     9721 gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc
     9781 gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa
     9841 attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc
     9901 agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct
     9961 caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg
    10021 actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa
    10081 atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct
    10141 aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat
    10201 cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc
    10261 atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat
    10321 aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt
    10381 tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt
    10441 agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag
    10501 gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag
    10561 gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca
    10621 tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat
    10681 ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag
    10741 acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga
    10801 tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga
    10861 acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat
    10921 ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt
    10981 gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt
    11041 agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt
    11101 aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc
    11161 tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata
    11221 gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga
    11281 acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt
    11341 tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt
    11401 cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg
    11461 atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat
    11521 ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat
    11581 cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc
    11641 agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac
    11701 aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca
    11761 aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac
    11821 aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg
    11881 gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt
    11941 tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag
    12001 tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc
    12061 ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt
    12121 gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga
    12181 ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga
    12241 ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg
    12301 aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca
    12361 tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag
    12421 agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag
    12481 aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg
    12541 ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc
    12601 caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg
    12661 aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg
    12721 gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc
    12781 atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga
    12841 ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta
    12901 ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca
    12961 aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca
    13021 tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac
    13081 gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa
    13141 caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat
    13201 tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt
    13261 tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga
    13321 tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa
    13381 agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt
    13441 cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt
    13501 aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa
    13561 ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct
    13621 agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg
    13681 tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga
    13741 atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc
    13801 accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc
    13861 gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt
    13921 ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc
    13981 aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga
    14041 tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga
    14101 aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca
    14161 aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga
    14221 agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg
    14281 agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct
    14341 tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc
    14401 aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt
    14461 gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta
    14521 tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg
    14581 tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc
    14641 ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca
    14701 tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa
    14761 aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca
    14821 attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac
    14881 acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga
    14941 tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat
    15001 cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc
    15061 gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga
    15121 ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt
    15181 gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga
    15241 agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt
    15301 agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg
    15361 gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt
    15421 ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa
    15481 ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt
    15541 ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc
    15601 cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct
    15661 gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca
    15721 ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac
    15781 tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat
    15841 ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg
    15901 gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca
    15961 cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa
    16021 aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt
    16081 caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga
    16141 tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag
    16201 gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa
    16261 ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa
    16321 caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg
    16381 gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga
    16441 atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct caaggtatgt
    16501 atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac ggtgtattcc
    16561 aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc
    16621 gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac ttcacattga
    16681 catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa tcagcggacg
    16741 gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt gttggtctaa
    16801 cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca
    16861 tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc
    16921 ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg ttcatttatc
    16981 caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg tttgtttcaa
    17041 aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata gaaatcgtta
    17101 ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa tattaccaaa
    17161 gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc aggagaaatt
    17221 catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt gctttccgga
    17281 ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa gatcacaagt
    17341 ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat gcttgttgat
    17401 gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt
    17461 gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg attgtcagtc
    17521 aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt agaaatttta
    17581 aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc tgtattgacc
    17641 cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg
    17701 atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg ctgtacagtt
    17761 atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc acaacaatta
    17821 gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct
    17881 caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc
    17941 gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat
    18001 ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc agaaagcgga
    18061 catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat cactgaacat
    18121 ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga tatgacatta
    18181 tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa cggcatgctg
    18241 aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga tgttcgaaca
    18301 acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa agcaattatc
    18361 gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac tcgtggactg
    18421 gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga caaatacaaa
    18481 gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga tcgtattgct
    18541 gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa
    18601 ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt agaaaagggg
    18661 gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc ctgtactttc
    18721 agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt ttcagcccat
    18781 ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga taggtgaaac
    18841 gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag ctaactcagc
    18901 tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg tcagtgtctg
    18961 ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat gcgtgatctt
    19021 aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag cattttttgg
    19081 aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg taagcacatt
    19141 tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt ccaatatgat
    19201 cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg gctctcgcgt
    19261 aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc tgatgaagaa
    19321 aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt ctgagtatgc
    19381 aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg ctttggcagg
    19441 gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc aaacgacttc
    19501 gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat cagcaatcgg
    19561 tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg gcatgcagac
    19621 aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta tcttctttgt
    19681 cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga
    19741 agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact
    19801 ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt ttattcaaca
    19861 ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat cgtaaacgta
    19921 gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa cttgacgatg
    19981 agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt
    20041 atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga ccatattatc
    20101 agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat caaagcatgg
    20161 tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc tttcccagga
    20221 ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt acctgcttat
    20281 tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa attcggcttg
    20341 cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat caatgtatat
    20401 gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg cggtgctgtt
    20461 tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca agggttcatc
    20521 tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg tgcctcattg
    20581 ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt tatttcttcg
    20641 attccgccag tattgctgca aattgctcca tatttgctga caatcattgt gctggttgtt
    20701 ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa atcaaaataa
    20761 tcttttagcg aataaaaagg gtgggttacg g
//
LOCUS       DAPCMU010000375        17103 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.053, whole genome shotgun sequence.
ACCESSION   DAPCMU010000375 DAPCMU010000000
VERSION     DAPCMU010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.053"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     misc_binding    55..271
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            319..2964
                     /locus_tag="RD744_003026"
     CDS             319..2964
                     /locus_tag="RD744_003026"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDU0558928.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3274..4587
                     /locus_tag="RD744_003027"
     CDS             3274..4587
                     /locus_tag="RD744_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDU0558929.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4577..5230
                     /locus_tag="RD744_003028"
     CDS             4577..5230
                     /locus_tag="RD744_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDU0558930.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5285..5836
                     /gene="radC"
                     /locus_tag="RD744_003029"
                     /pseudo
     CDS             5285..5836
                     /gene="radC"
                     /locus_tag="RD744_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            5967..7052
                     /locus_tag="RD744_003030"
     CDS             5967..7052
                     /locus_tag="RD744_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0558931.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7049..8941
                     /locus_tag="RD744_003031"
     CDS             7049..8941
                     /locus_tag="RD744_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDU0558932.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8948..9325
                     /locus_tag="RD744_003032"
     CDS             8948..9325
                     /locus_tag="RD744_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDU0558933.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9479..10261
                     /locus_tag="RD744_003033"
     CDS             9479..10261
                     /locus_tag="RD744_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDU0558934.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10387..11118)
                     /gene="erm(A)"
                     /locus_tag="RD744_003034"
     CDS             complement(10387..11118)
                     /gene="erm(A)"
                     /locus_tag="RD744_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDU0558935.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11176..11235)
                     /locus_tag="RD744_003035"
     CDS             complement(11176..11235)
                     /locus_tag="RD744_003035"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDU0558936.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            11627..12289
                     /locus_tag="RD744_003036"
     CDS             11627..12289
                     /locus_tag="RD744_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDU0558937.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            12524..12667
                     /locus_tag="RD744_003037"
                     /pseudo
     CDS             12524..12667
                     /locus_tag="RD744_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            12817..13017
                     /locus_tag="RD744_003038"
     CDS             12817..13017
                     /locus_tag="RD744_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDU0558938.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(13271..14950)
                     /locus_tag="RD744_003039"
     CDS             complement(13271..14950)
                     /locus_tag="RD744_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDU0558939.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(14952..15164)
                     /locus_tag="RD744_003040"
     CDS             complement(14952..15164)
                     /locus_tag="RD744_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDU0558940.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            15557..>17103
                     /gene="efrA"
                     /locus_tag="RD744_003041"
     CDS             15557..>17103
                     /gene="efrA"
                     /locus_tag="RD744_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDU0558941.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKK"
BASE COUNT         5686 a         2721 c         3523 g         5173 t
ORIGIN      
        1 agtgtttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata
       61 acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta tctgctgaaa
      121 ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat ggagtagaga
      181 tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta ttgcaattta
      241 ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac gttttttagt
      301 ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata taatccgcaa
      361 gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa accgagcgga
      421 gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac tggaaaactt
      481 catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca aaagagaatg
      541 caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc gacacaagca
      601 aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg tcgtgaaaag
      661 ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg cgaacagtgg
      721 gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga tgatggatta
      781 tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt
      841 gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat cgaagttatt
      901 cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga tggatcaggc
      961 gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc gattgctgtt
     1021 catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct acctttagtc
     1081 aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg gacaggtgta
     1141 gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg tcatgatctt
     1201 ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg aaaatatgct
     1261 ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga
     1321 ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt
     1381 gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc
     1441 attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt caatcaaacg
     1501 tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt atggtgggga
     1561 catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca
     1621 ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg gtttagttca
     1681 gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga ttataagcga
     1741 tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg ggtaagcaga
     1801 atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt cttgattcat
     1861 ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa tggaatcgat
     1921 ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt atcgaatggt
     1981 tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt
     2041 atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg aatgaccgta
     2101 gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct tactcgtttg
     2161 aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt
     2221 cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga aatgagtaaa
     2281 gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat cttagtccat
     2341 acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac agaagaaata
     2401 tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc tgttgtacgt
     2461 ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga attgattcga
     2521 gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat tacgttgcta
     2581 attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag ctatatcgaa
     2641 cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc agatctagcg
     2701 atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt
     2761 gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt
     2821 gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga agtagtagag
     2881 gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa agaacgaatc
     2941 gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca gctagctatt
     3001 actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca ctcttagctt
     3061 cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc tactcttatt
     3121 atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag ttgataaaat
     3181 cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat gtgttctatt
     3241 tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc aatagaatgg
     3301 atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga agctttatta
     3361 gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg aacaaacgga
     3421 aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct gacagtaggt
     3481 acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc
     3541 attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat caaacaactt
     3601 gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc aatggcattt
     3661 gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt aggcggctta
     3721 ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat tggacttgat
     3781 cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc
     3841 aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac tgtcatccaa
     3901 caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta taaaataaac
     3961 tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga aataggaaag
     4021 atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc aggagtagcc
     4081 atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt
     4141 ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag taacgagcca
     4201 ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt tgagaatttg
     4261 aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg aactaaaaat
     4321 gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta tttgacgacc
     4381 tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag tgagagagta
     4441 tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat gacaaatgaa
     4501 gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca tttattaata
     4561 caattaggag gagatcatga agtataatgc agtgattttt gacatggatg gtttgctgtt
     4621 cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga tgggttttcc
     4681 ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag tatgggcgaa
     4741 ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca tagatgattc
     4801 ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc ccggtgtgtt
     4861 ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt ccagtaacca
     4921 acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat ttgagacgat
     4981 tgtttctgca gaaaatgttc aacgagcaaa accagaccta gaaattttct tgttagctaa
     5041 tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac aaaatggtat
     5101 tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc ttccaccatc
     5161 ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa tccctgcatt
     5221 gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga aaggagatgt
     5281 ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa gggaacgtat
     5341 ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct tactgcgtac
     5401 cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg
     5461 actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag ggataggacg
     5521 cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc agacggatca
     5581 tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat tgattctgga
     5641 aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga agaatcaagt
     5701 gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc atcctagaga
     5761 aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc ataatcatcc
     5821 ttctggtaat gttttaacca tagtataata aataatagta gttaaagtag tttaagagga
     5881 gatattaaag ggatatttta ctttaaataa aaattaacaa ggtttataat ttgtagtagt
     5941 taaagtattt aaagaggtgg gaacatgtga aggttcaaag gatagaagtg gagaataagc
     6001 cgtatccatt gtatttatta ctagataaag aataccagct aatagaacca gtaatgaaat
     6061 ttattaaata cttagataat actggtaagt ctcctaatac cattaaagca tactgctatc
     6121 atttaaagtt gctgtacgag ttcatggaac agagaggtgt tattcttaat gatattaact
     6181 ttgagttgtt agcagacttc gtaggttggt tgagatatcc ttcagcatca aatgtaattg
     6241 atcttcagtc aaaaaaagcc ataagagaag aaacgacagt gaatacaatt ttaaatgtag
     6301 ttatgagttt tcttgattat ttaagtagat taggagaatt taaatcaatt gatgtattta
     6361 aacaagcaaa gggaagaaat ttcaaaggat ttttacatca tgttaataag ggtagatacc
     6421 aaaagaatgt cttaaagtta agggttaaaa agaaacagat aagaacattg agatcagagg
     6481 aagttaagca aattattgat gcttgtcata cgaaaagaga taaattaatt ttaatgctta
     6541 tgtatgaggg tggtttaaga atcggtgaag tgttatcgct taggcttgaa gatattgtca
     6601 cttgggacaa tcaaatccat ttaacaccta gagatgttaa tgttaatgaa gcttatatta
     6661 aattaaggaa ggaaagaaca atacatgtga gtaaagaact tatgtcactt tatacagatt
     6721 acttgatata tgagtatagt gaggaattgg agcatgatta tgtttttatt tccttaaaag
     6781 aaggctattt tgggaaacca ctaaagtacc aaagtgttct tgatctagtt agaagaatag
     6841 ttaaaaggac tggaatagaa tttacatcac atatgcttcg ccacactcac gcaacgcagc
     6901 taattaggga aggatgggat gttgcgttcg ttcaaaagag attaggtcac gcacatgttc
     6961 agacaacgtt aaatacctat gttcatcttt cagatcagga tatgaaaaat gagtttaata
     7021 aatacctcga gagaaaggag cataagaaat gaatgcttct agtaaaagga aaattattag
     7081 tcagagtgag attagcaaaa aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc
     7141 taataatagt tgggatataa gaaaatgtcc acatccttct gccatagaat taagtaagaa
     7201 tcctgcttta aggaatcgtt gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac
     7261 agaattgaaa tatttttatt tttaccattt aaacaatgga atatggaatg caaaaactgt
     7321 ctggattaga aaaggaacag taattaataa aatgttagat ttcttagatt taaagtatcc
     7381 tagcattact tcaattactg aagttcctat tgaaaaagca atgacggagt atagaactta
     7441 tttgacaaaa cggggtgtta gaattaccac tactaattat aagattactg ctaatcaaga
     7501 aaaaacacct gtaaaagcta attcctacta tgttactaat ctaaaacaat ttatggagtt
     7561 ttatgagaac ttttattttg atggagagga gtgggataaa gacgtttggg atagacgtaa
     7621 cttacctttg ccagatgata aggttaaccc aacacaatat gaatatacaa ttaactttaa
     7681 agggtttcgg aatacatatt ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa
     7741 catggatagc ttttcctatg taagtgatat tgcccaaaga cttaaagagt tctttaattt
     7801 tctggacatg aaatttaaac aagttcagag agtacaccaa ttaacgagag tggaaattga
     7861 agcatattta agtgaactaa acatgatggg aataaaacct agtacaataa ctgggaggat
     7921 ctctatattg gaaggactat ttagtaccct tcttaggcta gaatgggatg atgttccttc
     7981 caaaatatta atttattctg aggactatcc gaaaatacca agagcaaaac cacgctttat
     8041 agatgaattc gtcctagagc aattgaacag tcatcttgat aaattacccg aatatatagc
     8101 tacgatgact atgattgttc aagaatgtgg aatgaggata agtgaattgt gcaccttgaa
     8161 aaaaggctgt ctattagagg acaaagatgg agatttcttt ttaaagtatt atcaatggaa
     8221 aatgaaaaag gagcatatag ttccaatatc taaagaggta gctttactta ttaaagttcg
     8281 ggaagataaa gtttcagagg aatttccaga tagtgaatac ctctttccaa gaaaagatgg
     8341 atcgccatta aaacaagaaa catttagagg tgagttaaat aaattagctt atgagcaaaa
     8401 tatagtggat aaatcaggtg agatttatag attccatgcc catgcctttc gccatacagt
     8461 aggaacaaga atgattaaca acgggatgcc ccagcatatt gtgcagaaat ttttggggca
     8521 tgaaagccca gaaatgacaa gcagatacgc tcatatcttt gatgaaactc taaaaaatga
     8581 atttactaaa tttcaggaaa aactggttac caataatgga gatgtgcttg atctagatga
     8641 agataatgaa gtcgatgatg tagagcttca atggttcaag aaaaatataa atgcacaagt
     8701 gcttccaaat ggttattgta gattgccagt agtagcaggt ggttgtccac atgcgaatgc
     8761 atgcttagat tgcactcact tctgtaccag taagcaattc ttaccacagc acgaagaaca
     8821 gttagagcgt acagaagagt tattagccat agctaaggat aaacaatggc aaagacaagt
     8881 agagactaat agccgtgtta aagagcgttt agaacaaatc attggaagtt tgacggggta
     8941 attatcaatg gataaacaag ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa
     9001 atcaaaaaag acaagggaaa aagtagacaa agcgatttct aaattttcga ttgaaggtaa
     9061 agttattaat tttaattcaa tagcaaagga agctaatgtt tctaaatcat ggctttataa
     9121 ggaacacgat attaggcaaa gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt
     9181 agtctcaaaa cccaagaaaa gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag
     9241 aagagtaatg gaattagaaa aagaaaataa aaaattacag aaccaaattc aaaaattata
     9301 tggagatctg tataataaag aataattatt aatctgtaga caaattgtga aaggatgtac
     9361 ttaaacgcta acggtcagct ttattgaaca gtcatttagg tatatgtcca atctagggta
     9421 agtaaattga gtatcaatat aaactttata tgaacataat cagttacgag gtgaaatcat
     9481 gagcaatttg attaacggaa aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa
     9541 agatttattt ggaagttcaa tagttggagt atatctattt ggttcagcag taaatggtgg
     9601 tttacgcatt aacagcgatg tagatgttct agtcgtcgtg aatcatagtt tacctcaatt
     9661 aactcgaaaa aaactaacag aaagactaat gactatatca ggaaagattg gaaatacgga
     9721 ttctgttaga ccacttgaag ttacggttat aaataggagt gaagttgtcc cttggcaata
     9781 tcctccaaaa agagaattta tatacggtga gtggctcagg ggtgaatttg agaatggaca
     9841 aattcaggaa ccaagctatg atcctgattt ggctattgtt ttagcacaag caagaaagaa
     9901 tagtatttct ctatttggtc ctgattcttc aagtatactt gtctccgtac ctttgacaga
     9961 tattcgaaga gcaattaagg attctttgcc agaactaatt gaggggataa aaggtgatga
    10021 gcgtaatgta attttaaccc tagctcgaat gtggcaaaca gtgactactg gtgaaattac
    10081 ctcgaaagat gtcgctgcag aatgggctat acctctttta cctaaagagc atgtaacttt
    10141 actggatata gctagaaaag gctatcgggg agagtgtgat gataagtggg aaggactata
    10201 ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct atagaaactt ctctcaatta
    10261 ggctaatttt attgcaataa caggtgctta cttttaaaac tactgattta ttgataaata
    10321 ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa attagagaac gctttattac
    10381 tttaatttag tgaaacaatt tgtaactatt gaaaatagaa agaaattgtt ccttcgatag
    10441 tttattaata ttagtgacat ttgcatgctt caaagcctgt cggaattggt ttttagtgaa
    10501 aagaacacga tattcacggt ttacccactt ataaacaaaa gatcgatact ttttgtagtc
    10561 cttctttgaa atcaatggtt gatgtcgttc aagaacaatc aatacagagt ctacacttgg
    10621 cttaggatga aaatatagtg gtggtacttt tttgagcatt tttatatcca tctccaccat
    10681 taatagtaaa cccaaagctc gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac
    10741 gataagatag ctatatttag cctgactttc aaaggtaatt cttttgacaa tatccgtact
    10801 gatgttataa ggaatattac catatatctt atagtttata tgttttggga aggaaaattt
    10861 tagaatatcc gtttgaatca cttttatatt ctcagagggg tttaccgctt ctttagtcac
    10921 ttgacataag cctccatcaa tttctatagc agtaactgat cgactcattt tgactagctc
    10981 tttggtaaaa tgtccttttc ctgatccgat ttctattacg ttgtcttgtt tactgatatt
    11041 cgtgtgattc aatatttctt ttacatgctt tttagaagta ataaaatttt gcgtgtcttt
    11101 agggtttttc tggttcatta taaccttctc cttactggtt ataatgaact gacattaagc
    11161 gttcattata accaattaat tttgatttgg ttgataacga actttattaa taacaaatat
    11221 agaaaaagta cccatacttt tatctccttt ccattttttt cattatgaat gagataaagt
    11281 aatttctact actgcgatac tggtgcacat aattgaaacc tccttatgta actgaatatg
    11341 attgtatcat cttttcattt tagaataaac ctttatatgt aattttgcac ttgacacttc
    11401 agatggacgt tttcttcaaa ataatatgtt ttgaaacttg ataagagaag ccactgtctt
    11461 tactaaaaag taaaggctgc gataaaggac agtgacttta ataaagttgc actgttgtat
    11521 tgtctttgga taaaattgtc taaagggaag ttttatcccc aacgaaaaaa catctaaagt
    11581 ggtaagtaat gaaagaatgt cgataaagta ggaatggggg atctttttga atacaataga
    11641 atcacagttt gataaggttg cagaagatta cgatttcgtg aatgagcttt tgaatgatta
    11701 ttcattcttt gtgtctaata tgtctccaaa gaaaggcaga gcattagata tcggatgtgg
    11761 ctcgggtttg ttagtggaga aattagcaag ttattatgat gaagtggtag ggattgatat
    11821 ttctaatcaa atgctcgatc ttgccaaatc taaacgtcaa ctaacaaata cggtctatct
    11881 gaatatgaat gcagaacaac ttaattttaa tgagaagttt gattttattg taagccgaac
    11941 aacctttcat catttggatg atatagccag cgtaatacag caaatgaagg aactgttgaa
    12001 tgaggaagga agaatagtta ttcttgataa tgtatctgaa gtcgagacac cgcctaccta
    12061 tgtttataaa ttaggggcaa tccaagagtt tttaccgcat tgtttcaaat ttggaataaa
    12121 aaatgcgatt agaatttata atcacaacac atctaaatca tggcttgagc atttagcttc
    12181 agataagtac ctatctgaac aaaattatta cgatttatat gaaaagttgt tgcctggatg
    12241 ccaattccat aaaatgggtt gggcaatggg ggttgtctgg acaaaataac ttaaagctgt
    12301 ggctttgaaa agttgaattt gtgattgttt tcagagctat cgggtgcttt agtataaata
    12361 acaatgaata aaaaatcagc ctgtatctta tgttactatt aagtaattta agatacaggt
    12421 tttactattt ttgaaataag taccttccac ttcaaataca tgtattcaaa gtgaaagtat
    12481 tgatttaaat gattcttttg taatttactc ttgataatat gaaattccat cgcagcagga
    12541 tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca ctgtactgga
    12601 ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa tgatggaaga
    12661 gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat tgtaattttg
    12721 tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca atgttacaat
    12781 ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac agtaaaatgg
    12841 ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga cgtattcgtt
    12901 cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca agcagtgact
    12961 ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa agcttaattt
    13021 tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa gcgagtaaca
    13081 aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt atgaaaaagt
    13141 aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga acaaacaaaa
    13201 atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg ctgatttcat
    13261 aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt ggatgtcttt
    13321 ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc aatttatatt
    13381 ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt cgctgagctt
    13441 cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga atatctggac
    13501 cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca agaccttctt
    13561 ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc ccgctaccat
    13621 cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg
    13681 ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct acatcttgtg
    13741 ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata aaatattttg
    13801 gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg gaagtatgga
    13861 tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa ttgatcaaat
    13921 gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact gtatcgtctg
    13981 gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt
    14041 ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga ttgatttcgt
    14101 gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga ccgttgacga
    14161 tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc acagcggcat
    14221 ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg atacgtcctt
    14281 cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat ttagtaaatg
    14341 ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct tccccgatac
    14401 gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat ttgaagtctc
    14461 cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca tctgggatac
    14521 tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc gtatcttcat
    14581 tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg tttgtgatca
    14641 acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc aagtagggta
    14701 ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt
    14761 tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa tcgtcttccg
    14821 ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc ccatacgtat
    14881 ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta tttttgatgg
    14941 aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc gtactctaga
    15001 tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc ttccacacgt
    15061 tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc ttcccgttta
    15121 gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt gtctccttta
    15181 tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa cgaggggaca
    15241 agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa agagacaagc
    15301 ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat aaaaaatata
    15361 caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc aactagattg
    15421 aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt atgagagaag
    15481 tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta acaaaagatt
    15541 aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata agaaattctt
    15601 gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac ttcctacgat
    15661 tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg tgaagcaaca
    15721 agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct tgttaggtta
    15781 ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa
    15841 gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat tgatcactcg
    15901 tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac gtatcggttt
    15961 tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact
    16021 cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag cgattgctaa
    16081 ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca acagtatctt
    16141 gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga
    16201 aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct tattccgttt
    16261 gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg tattgatcat
    16321 ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt
    16381 tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg tgttcatgat
    16441 gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg ccaaaccttc
    16501 gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata tcgaatttca
    16561 aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa atgtcagttt
    16621 tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg gaaaatctac
    16681 gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg
    16741 agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt ttattccgca
    16801 aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga aagaagatgc
    16861 tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat tcatttctcg
    16921 aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt caggtggaca
    16981 aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct atatttttga
    17041 tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa
    17101 aga
//
LOCUS       DAPCMU010000376        13213 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.074, whole genome shotgun sequence.
ACCESSION   DAPCMU010000376 DAPCMU010000000
VERSION     DAPCMU010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.074"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(204..2162)
                     /locus_tag="RD744_003042"
     CDS             complement(204..2162)
                     /locus_tag="RD744_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDU0558942.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="RD744_003043"
     CDS             complement(2159..3031)
                     /locus_tag="RD744_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDU0558943.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="RD744_003044"
     CDS             complement(3373..4245)
                     /locus_tag="RD744_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDU0558944.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="RD744_003045"
     CDS             4373..4942
                     /locus_tag="RD744_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDU0558945.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="RD744_003046"
     CDS             5009..5746
                     /locus_tag="RD744_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDU0558946.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="RD744_003047"
     CDS             5977..7245
                     /locus_tag="RD744_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDU0558947.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="RD744_003048"
     CDS             7732..8196
                     /locus_tag="RD744_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDU0558948.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="RD744_003049"
     CDS             8180..8809
                     /locus_tag="RD744_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDU0558949.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="RD744_003050"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="RD744_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDU0558950.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="RD744_003051"
     CDS             10688..11134
                     /locus_tag="RD744_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDU0558951.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="RD744_003052"
     CDS             complement(11333..11848)
                     /locus_tag="RD744_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDU0558952.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="RD744_003053"
     CDS             11965..13167
                     /locus_tag="RD744_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDU0558953.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAPCMU010000377         7959 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.123, whole genome shotgun sequence.
ACCESSION   DAPCMU010000377 DAPCMU010000000
VERSION     DAPCMU010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.123"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            128..1564
                     /locus_tag="RD744_003054"
     CDS             128..1564
                     /locus_tag="RD744_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDU0558954.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="RD744_003055"
     CDS             1753..4200
                     /locus_tag="RD744_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDU0558955.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="RD744_003056"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="RD744_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDU0558956.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="RD744_003057"
     CDS             complement(5690..6793)
                     /locus_tag="RD744_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDU0558957.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD744_003058"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="RD744_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDU0558958.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAPCMU010000378         6386 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.142, whole genome shotgun sequence.
ACCESSION   DAPCMU010000378 DAPCMU010000000
VERSION     DAPCMU010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.142"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            58..1977
                     /gene="tet(M)"
                     /locus_tag="RD744_003059"
     CDS             58..1977
                     /gene="tet(M)"
                     /locus_tag="RD744_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDU0558959.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVE
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2087..2137
                     /locus_tag="RD744_003060"
                     /pseudo
     CDS             2087..2137
                     /locus_tag="RD744_003060"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2171..3547
                     /gene="tet(L)"
                     /locus_tag="RD744_003061"
     CDS             2171..3547
                     /gene="tet(L)"
                     /locus_tag="RD744_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDU0558960.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKSPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGALIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPTITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIMVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            4111..5373
                     /locus_tag="RD744_003062"
     CDS             4111..5373
                     /locus_tag="RD744_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDU0558961.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5618..6370
                     /locus_tag="RD744_003063"
     CDS             5618..6370
                     /locus_tag="RD744_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDU0558962.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKGSVAKF"
BASE COUNT         2210 a          916 c         1312 g         1948 t
ORIGIN      
        1 agtatttatc actgggattt ttatgccctt ttgggttttt gaatggagga aaatcacatg
       61 aaaattatta atattggagt tttagctcat gttgatgcag gaaaaactac cttaacagaa
      121 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
      181 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      241 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      301 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      361 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      421 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      481 caggatatta aagagaaact ttctatggaa attataatca aacagaaagt agagctgcac
      541 cctaatatgt gtgtgatgag ctgtacggaa cctgagcaat gggatgtggt aatagaagga
      601 aatgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt agaactcgaa
      661 caagaggaaa tcagaagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      721 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      781 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      841 agacaacgtc ttgcatatgt acgcctttat agtggagtac tacatttgcg ggattcggtt
      901 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      961 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
     1021 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
     1081 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1141 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta tgtggattct
     1201 acgacacatg aaatcatact ttctttctta gggaaagtac aaatggaagt gacttgtgct
     1261 ctattgcaag aaaagtatca tgtggaggta aaaataaaaa agcctacagt catttatatg
     1321 gaaagaccgt taaaaaaagc agagtatacc attcacatcg aagtgccacc gaatcccttc
     1381 tgggcttcca ttggtctttc tgtagaaccg cttccattag ggagcggagt acagtatgag
     1441 agctcggttt ctcttggata cttaaatcaa tcgtttcaaa atgcagttat ggaagggata
     1501 cgatatggct gtgaacaagg attgtatggt tggaatgtga cggactgtaa aatctgtttt
     1561 aagtatggct tatactatag ccctgttagt accccagcag attttcggat gcttgctcct
     1621 attgtattgg aacaagtctt aaaaaaagct ggaacagaat tgttagagcc atatcttagt
     1681 tttaaaattt atgcgccaca ggaatatctt tcacgagcat acaacgatgc tcctaaatat
     1741 tgtgcgaaca tcgtagacac tcaattgaaa aataatgagg tcattcttag tggagaaatc
     1801 cctgctcggt gtattcaaga atatcgtagt gatttaactt tctttacaaa tggacgtagt
     1861 gtttgtttaa cagagttaaa agggtaccat gttactaccg gtgaacctgt ttgccagccc
     1921 cgtcgtccaa atagtcggat agataaagta cgatatatgt tcaataaaat aacttagtgt
     1981 attttatgtt gttatataaa tatggtttct tgttaaataa gatgaaatat tttttaataa
     2041 agatttgaat taaagtgtaa aggaggaatt aattatgaag tgaaatgaat gtaacagggt
     2101 tcaattaaaa gagggaagcg tatcattaac cctataaact acgtctgccc tcattattgg
     2161 agggtgaaat gtgaatacat cctattcaca atcgaattta cgacacaacc aaattttaat
     2221 ttggctttgc attttatctt tttttagcgt attaaatgaa atggttttga acgtctcatt
     2281 acctgatatt gcaaatgatt ttaataaatc acctgcgagt acaaactggg tgaacacagc
     2341 ctttatgtta accttttcca ttggaacagc tgtatatgga aagctatctg atcaattagg
     2401 catcaaaagg ttactcctat ttggaattat aataaattgt ttcggggcgt taattgggtt
     2461 tgttggccat tctttctttt ccttacttat tatggctcgt tttattcaag gggctggtgc
     2521 agctgcattt ccagcactcg taatggttgt agttgcgcgc tatattccaa aggaaaatag
     2581 gggtaaagca tttggtctta ttggatcgat agtagccatg ggagaaggag tcggtccagc
     2641 gattggtgga atgatagccc attatattca ttggtcctat cttctactca ttcctacgat
     2701 aacaattatc actgttccgt ttcttatgaa attattaaag aaagaagtaa ggataaaagg
     2761 tcattttgat atcaaaggaa ttatactaat gtctgtaggc attgtatttt ttatgttgtt
     2821 tacaacatca tatagcattt cttttcttat cgttagcgtg ctgtcattcc tgatatttgt
     2881 aaaacatatc aggaaagtaa cagatccttt tgttgatccc ggattaggga aaaatatacc
     2941 ttttatgatt ggagttcttt gtgggggaat tatatttgga acagtagcag ggtttgtctc
     3001 tatggttcct tatatgatga aagatgttta ccagctaagt actgccgaaa tcggaagtgt
     3061 aattattttc cctggaacaa tgagtgtcat tattttcggc tacattggtg ggatacttgt
     3121 tgatagaaga ggtcctttat acgtgttaaa catcggagtt acatttcttt ctgttagctt
     3181 tttaactgct tcctttcttt tagaaacaac atcatggttc atgacaatta taatggtatt
     3241 tgttttaggt gggctttcgt tcaccaaaac agttatatca acaattgttt caagtagctt
     3301 gaaacagcag gaagctggtg ctggaatgag tttgcttaac tttaccagct ttttatcaga
     3361 gggaacaggt attgcaattg taggtggttt attatccata cccttacttg atcaaaggtt
     3421 gttacctatg gaagttgatc agtcaactta tctgtatagt aatttgttat tacttttttc
     3481 aggaatcatt gtcattagtt ggctggttac cttgaatgta tataaacatt ctcaaaggga
     3541 tttctaaatc gttaagggat caactttggg agagagttca aaattgatcc tttttttata
     3601 acaggaattc aaatcttttt gttccattaa agggcgcgat tgctgaataa aagatacgag
     3661 agacctctct tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa
     3721 agacaataaa aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca
     3781 aaataaaaat tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata
     3841 ctacctgtcc cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt
     3901 ggcagagggc aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt
     3961 tttatggttt tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa
     4021 gtatagtgtg ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat
     4081 ttgtgccacc taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa
     4141 gtgaaatcag ctggactaaa aggcatgcaa tttcataatc aaagagagcg aaaaagtaga
     4201 acgaatgatg atattgacca tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa
     4261 aatattgatt acaacgaacg tgtcaaagaa attattgaat cacaaaaaac aggtacaaga
     4321 aaaacgagga aagatgctgt tcttgtaaat gagttgctag taacatctga ccgagatttt
     4381 tttgagcaac tggatccagg agaacaaaaa cgattttttg aggaaagtta taaattattt
     4441 tccgaacgat atggcaagca aaatattgct tatgcaacag ttcataatga tgagcaaacc
     4501 cctcacatgc atttaggtgt tgtgcctatg cgtgatggaa aactgcaagg aaaaaatgtg
     4561 tttaatcgtc aagaactgtt atggctacaa gataaattcc ccgagcatat gaaaaaacag
     4621 ggttttgagt tgaagcgtgg tgaacgtggc tctgaccgta aacatattga gacagctaaa
     4681 tttaaaaaac aaactttgga aaaagagatt gattttctag aaaaaaattt agcagttaaa
     4741 aaagatgaat ggactgctta tagcgataaa gttaaatcag atttagaagt accagcgaaa
     4801 cgacacatga aaagtgttga agtgccaacg ggtgaaaagt ccatgtttgg tttgggaaaa
     4861 gaaataatga aaacagaaaa gaaaccaacc aaaaatgttg ttatatcgga gcgtgattat
     4921 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
     4981 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
     5041 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
     5101 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
     5161 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
     5221 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
     5281 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
     5341 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
     5401 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
     5461 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
     5521 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgaccaa
     5581 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
     5641 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
     5701 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
     5761 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
     5821 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
     5881 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
     5941 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
     6001 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
     6061 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
     6121 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
     6181 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
     6241 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
     6301 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg gttctgttgc
     6361 aaagttttaa ataaagaata aaatcc
//
LOCUS       DAPCMU010000379         3644 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.199, whole genome shotgun sequence.
ACCESSION   DAPCMU010000379 DAPCMU010000000
VERSION     DAPCMU010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.199"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            100..1221
                     /locus_tag="RD744_003064"
     CDS             100..1221
                     /locus_tag="RD744_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDU0558963.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1479..2981
                     /gene="eat(A)"
                     /locus_tag="RD744_003065"
     CDS             1479..2981
                     /gene="eat(A)"
                     /locus_tag="RD744_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDU0558964.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3009..3575
                     /locus_tag="RD744_003066"
     CDS             3009..3575
                     /locus_tag="RD744_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDU0558965.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1241 a          624 c          815 g          964 t
ORIGIN      
        1 tttattttaa atggctaagt tcattataaa atccgccatt gttttgtttg aaaaaaatga
       61 aaaaaagtgt cttacttaag aagaaagtga gggatacaga tggatatttt ggcggcaatt
      121 gattcattta aaggaagtgc ttcttcattt gaactaaatc aggcagttct aaatggctgg
      181 gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag atggcggaga aaatactgca
      241 gaagcaatct attacgcgct caggggaaca tggcgtacca ttgatgggat ggatctctta
      301 ttccgtcagc gaaaaatcag ctatttattg acaacttggc aaggaaaaaa caccgcagtc
      361 atcgaatctg cacaagcatt ggggttagat ctacttgata aaccaacgga tcaaactatc
      421 cgacttgctt ccagttatgc cttaggagaa cttattaaag atgcactgct tcaagaagta
      481 gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg atggtggtct aggattactc
      541 caatccttgg gtgcttcttt agaagggtac acagaaggaa atcctttatt atctactaaa
      601 aaacttcatt ttgatcgaag aaacgttctt gtttctaaag taaaattgat tatcgctgca
      661 gatgttacca atccatatac aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa
      721 ggaggaatag atacgacgct agctttctta gataaacaag caggaaagat tgctcagcaa
      781 ataaaagatt tctgggaggt ggatttagat gatattccag gaagtggagc agcaggcggt
      841 attggtggag cccttttttt gttgggagcg aagatggttt ctggtttcga tttagtcagc
      901 caaatcactg gattggaaaa aaagatcgaa caagcagatg tcatcattac aggagaaggg
      961 cggatcgatc agcaaacgat ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa
     1021 aaatatcgaa aaccaataat cgctatttgc ggaagtagag accaagagct agaaggactt
     1081 tctgattttc tcccaattgt cttaagtatt catttgggtc caataggttt agaagaagca
     1141 atggaacaca ggcaaacatt agaaaaaaca aaaatcttag gacagacact tagcagattt
     1201 ttcaccatcc tcccgaagta gttgacaaaa tcattttctt tatgctatcg taatttcaaa
     1261 tataggacaa agtgagggat ccgtgcattg aaaaattaag tcgattgatg aaagctgcat
     1321 tcagactgta ccttgtggag acaaggaagc agtgtgcaga ttttcgggat tggcttggtt
     1381 ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg catcctcttt agccagttct
     1441 gaaaatagga ctggtctttt tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa
     1501 atctgacatt cggctacgac agccaaggca cattattatt tgaacaagca aatctaaatt
     1561 ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac
     1621 tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg
     1681 cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata
     1741 ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg
     1801 aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg
     1861 cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg
     1921 atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg
     1981 tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa
     2041 aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc
     2101 gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga
     2161 aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac
     2221 aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg
     2281 ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg
     2341 agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag
     2401 cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt
     2461 tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta
     2521 acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta
     2581 taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag
     2641 acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt
     2701 tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga
     2761 tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac
     2821 tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac
     2881 aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg
     2941 ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac
     3001 aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa
     3061 cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc
     3121 cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc
     3181 ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag
     3241 cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt
     3301 ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt
     3361 tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt
     3421 tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct
     3481 tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca
     3541 tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa
     3601 aatatttcgg ctctttgtca ataaggactg atgagttgtg caaa
//
LOCUS       DAPCMU010000380         3192 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.205, whole genome shotgun sequence.
ACCESSION   DAPCMU010000380 DAPCMU010000000
VERSION     DAPCMU010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.205"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..212
                     /locus_tag="RD744_003067"
     CDS             <1..212
                     /locus_tag="RD744_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558966.1"
                     /translation="FDSRFKTLQQNRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYA
                     ESNDNYTTFRVELPAMPDLVDKRRS"
     gene            427..1395
                     /locus_tag="RD744_003068"
     CDS             427..1395
                     /locus_tag="RD744_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDU0558967.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1388..2419
                     /gene="vanA"
                     /locus_tag="RD744_003069"
     CDS             1388..2419
                     /gene="vanA"
                     /locus_tag="RD744_003069"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDU0558968.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2425..3033
                     /locus_tag="RD744_003070"
     CDS             2425..3033
                     /locus_tag="RD744_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDU0558969.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          949 a          600 c          783 g          860 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccggct ggacaatgct cgttcttccg
       61 atacgggtgg cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag
      121 ggcagattta cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga
      181 tgccagactt ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc
      241 tcaaggttat ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt
      301 tcttacacgg tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt
      361 tgacaagatt accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc
      421 ttaattatga ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca
      481 ttccatgctc tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg
      541 gaatccaacg ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag
      601 atttccgcct ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga
      661 agcatcggct gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac
      721 aatgtggcgt actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca
      781 gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc
      841 gaccgtggca aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc
      901 aaagcggtta ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc
      961 cgaagtatag aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt
     1021 acgcttcatg tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag
     1081 agaatgaagc aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat
     1141 gagttggtta aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa
     1201 ggagaggaag agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta
     1261 cttaaacttc aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag
     1321 caagcgttgc gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga
     1381 caggagcatg aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga
     1441 cgtatcggta aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc
     1501 gttatacatt ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga
     1561 atgggaaaac gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt
     1621 acttgttaaa aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt
     1681 gcatggcaag tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc
     1741 ttttgtaggc tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat
     1801 cgttgcgaaa aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag
     1861 gccggtggca gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc
     1921 cttcggtgtg aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag
     1981 acaatatgac agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc
     2041 ggtattggga aacagtgccg cgttagttgt tggcgaggtg gaccaaatca ggctgcagta
     2101 cggaatcttt cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcagttat
     2161 aaccgttccc gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa
     2221 aatatataaa gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa
     2281 cggccgcatt gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta
     2341 tccccgtatg atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt
     2401 attagcgtta aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta
     2461 cacggtgttc gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt
     2521 gacggttatg aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag
     2581 gcaaaagaac tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct
     2641 aagcgtgctg taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag
     2701 gaaagttatt atcccaatat tgaccgaact gagatgattt caaaaggata cgtggcttca
     2761 aaatcaagcc atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt
     2821 gagcttgtac caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca
     2881 aatggaatat catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac
     2941 agtgggtttg aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac
     3001 cccaatagct attttgattt ccccgttaaa taaactttta accgttgcac ggacaaacta
     3061 tataagctaa ctctttcggc aggaaacccg acgtatgtaa ctggttctta gggaatttat
     3121 atatagtaga tagtattgaa gatgtaaggc agagggttct gttgcaaagt tttaaataaa
     3181 gaataaaatc cc
//
LOCUS       DAPCMU010000381         2875 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.215, whole genome shotgun sequence.
ACCESSION   DAPCMU010000381 DAPCMU010000000
VERSION     DAPCMU010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.215"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(41..1027)
                     /locus_tag="RD744_003071"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="RD744_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="RD744_003072"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="RD744_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDU0558970.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..2838
                     /locus_tag="RD744_003073"
                     /pseudo
     CDS             1852..2838
                     /locus_tag="RD744_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
BASE COUNT          892 a          572 c          580 g          831 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacgg ttctgttgca aagttttaaa taaagaataa aatcc
//
LOCUS       DAPCMU010000382         2088 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.245, whole genome shotgun sequence.
ACCESSION   DAPCMU010000382 DAPCMU010000000
VERSION     DAPCMU010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.245"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            complement(772..1062)
                     /locus_tag="RD744_003074"
     CDS             complement(772..1062)
                     /locus_tag="RD744_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDU0558971.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1270..1962)
                     /locus_tag="RD744_003075"
     CDS             complement(1270..1962)
                     /locus_tag="RD744_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDU0558972.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          576 a          392 c          310 g          810 t
ORIGIN      
        1 ctattagtaa caagatcagt tctttgttat tcgttatttt tctattatgt ttgttcattt
       61 ttatactcct tcttatattt cctaatagag tataacatag ctttaagcat tacttaaaga
      121 taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa
      181 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
      241 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      301 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      361 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      421 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      481 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      541 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      601 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      661 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      721 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      781 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      841 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      901 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      961 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
     1021 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
     1081 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
     1141 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1201 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     1261 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     1321 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     1381 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt
     1441 ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga
     1501 aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc
     1561 atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag
     1621 ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt
     1681 tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc
     1741 ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt
     1801 atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc
     1861 tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag
     1921 aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc
     1981 caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt
     2041 acatgtgtaa ttttaatata aatactatca aatactcatt taatcctc
//
LOCUS       DAPCMU010000383         1364 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.289, whole genome shotgun sequence.
ACCESSION   DAPCMU010000383 DAPCMU010000000
VERSION     DAPCMU010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.289"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            187..270
                     /locus_tag="RD744_003076"
     CDS             187..270
                     /locus_tag="RD744_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDU0558973.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            395..1132
                     /gene="erm(B)"
                     /locus_tag="RD744_003077"
     CDS             395..1132
                     /gene="erm(B)"
                     /locus_tag="RD744_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDU0558974.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          510 a          215 c          218 g          421 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgtc ttgcgtatgg ttaaccctaa
       61 agttatggaa ataagactta gaagcaaact taagagtgtg ttgatagtgc attaaaaatt
      121 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga
      181 ttcgtcatgt tggtattcca aatgcgttat gtagataaaa catctactgt tttgaaacag
      241 actaaaaaca gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta
      301 atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt
      361 tgtttataga tgtaatcact tcaggagtga ttacatgaac aaaaatataa aatattctca
      421 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      481 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      541 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      601 aaaattaaaa ctgaatactc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      661 ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat
      721 tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg
      781 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      841 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      901 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      961 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
     1021 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga
     1081 gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga
     1141 gtcgcttttg taaatttgga aagttacacg ttactaaagg gaatgtagat aaattattag
     1201 gtatagggca cctctaataa ctaccaatta attcacctca aaatgaaatt agaaaaaaac
     1261 agcgaaattt ttaaatctat ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc
     1321 aacatgagga aatgttttta taaaaaaaga gtagttaccg tttt
//
LOCUS       DAPCMU010000384         1196 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.302, whole genome shotgun sequence.
ACCESSION   DAPCMU010000384 DAPCMU010000000
VERSION     DAPCMU010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.302"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="RD744_003078"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="RD744_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDU0558975.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DAPCMU010000385         1079 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.314, whole genome shotgun sequence.
ACCESSION   DAPCMU010000385 DAPCMU010000000
VERSION     DAPCMU010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.314"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            24..416
                     /locus_tag="RD744_003079"
     CDS             24..416
                     /locus_tag="RD744_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDU0558976.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1079
                     /gene="aac(6')-Ie"
                     /locus_tag="RD744_003080"
     CDS             417..>1079
                     /gene="aac(6')-Ie"
                     /locus_tag="RD744_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDU0558977.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNN"
BASE COUNT          432 a           85 c          190 g          372 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataat
//
LOCUS       DAPCMU010000386          888 bp    DNA     linear   BCT 30-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00076741
            SAMN33619559-rid17849413.guided.333, whole genome shotgun sequence.
ACCESSION   DAPCMU010000386 DAPCMU010000000
VERSION     DAPCMU010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33619559
            Sequence Read Archive: SRR23719553
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001710969.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001710969.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 04/30/2023 08:31:47
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,081
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,941
            CDSs (with protein)               :: 2,941
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 31 of 78
            Pseudo Genes (incomplete)         :: 37 of 78
            Pseudo Genes (internal stop)      :: 19 of 78
            Pseudo Genes (multiple problems)  :: 7 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33619559-rid17849413.guided.333"
                     /strain="AUSMDU00076741"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST1424"
     gene            <1..817
                     /locus_tag="RD744_003081"
                     /note="aph(2'')-Ia"
     CDS             <1..817
                     /locus_tag="RD744_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDU0558978.1"
                     /translation="GSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLE
                     TNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMH
                     GLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKK
                     CLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDIL
                     RMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          379 a           63 c          146 g          300 t
ORIGIN      
        1 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
       61 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
      121 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
      181 attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc
      241 tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca
      301 aatgcacggt ttagattata cagatattag tgaatgtact attgataata aacaaaatgt
      361 attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa
      421 agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa
      481 gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt
      541 aactggaata attgattttg gagattctgg aattatagat gaatattgtg attttatata
      601 cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat
      661 gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta
      721 tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta tcgaaaatgg
      781 tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat gaaaagctat
      841 tataaaagac ggttctgttg caaagtttta aatctactat caaataag
//
